Carg19221 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19221
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionLipoyl synthase, mitochondrial
LocationCarg_Chr19: 8428328 .. 8432261 (+)
RNA-Seq ExpressionCarg19221
SyntenyCarg19221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTTGGCGGGAAAACGCTTTACCAACGAACGTCCTTCATCTGTCCTGAGCTTCTCTACGGGGTCGCCAATCTCACGATGAACAGCCATGGCAGAAGCTTAGCTCGACTCCTAGAATCCGTTGGCAGATCCTTCTCTTCTTCATCTTCCTCAACCGCCGCCTCTGCAACCTCCTCCCAATTCTCTCGAACTTTGGCCGGTCTCCGTGAACGACTCGCCGTTGAGACCCCCTCACTTTCCGACTTCCTTGACCTGCAATCTTCTGAGTCGTATTCGGTGGAGGTTGGTACGAAGAAGAAGCCACTTCCCAAGCCTAAATGGATGAAGGAATCCGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAACTTCATACGGTTTGTGAGGAGGCTAGGTGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATACTTGGTGACACTTGTACCCGTGGTTGCAGGCTAGTGTTAATTCAATTTATTCATTCCATTTCGTTTGACTATTTAATTACCAATGTGTTTTTGTCATATAAATGGCCGTGTTGATTACTTTGATATCATCTCAGTGCCTCAGAACTTCTATTCATTCCATCGCTGTTTTGGGTTTGTTTGTAGTCCAGGTTCTGTAACGTCAAAACATCACGAACTCCGCCTCCACCAGACCCAAATGAGCCGAAAAATGTTGCTGAGGCAATCGCATCATGGGGCTTGGATTATGTGGTCATCACTAGTGTGGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGGTACTAATTCCCTAGATTCCTTTTCGTATCACAATGTCACTTTCATTGTTGCACTTAAGGATCCTTGACGTGACGCTTGATTTTTACGAGCCTTCTTTTATCTGGTTTCTATCAAACTAAGGAATTAAATGAAAGCCACTTCCATTGTTACATTTTTTTTTTTTTTTTGATGTTGCTTAACTAATTGATTTTGCAGTTCCTGATTTTCGAGGAGACCCCGGCTGTGTAGAAAATGTTGCAAAGTCAGGCCTCGACGTCTTTGCTCATAATATAGAGACAGTTGAGGAGCTTCAGGGAGCTGTTAGGGATCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCCCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAGACACCGGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTACATGAGACCTTCAAAGCGCCATATGCCTGTATCAGAATACATCACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGTACAGTCTCTTTCTTATTATTTGAACATTTTGGTTGTGTTCTGTCTTTGTTCTGCAAGAGATTGTTTAATCAAGCCCTTAAACCCTTTTTAAATCCCGCTTAAAAACACTTTTTTTTCTTTCACCTAAACAATTTTAAGCCCTCAGATAATCAATCCAATCAGGCTCGTAATCTGGTACGCTGCGGATAATTTTCATTTGCATTTCTTATATGTTCCTTCTCTAGGATTTGTATCGTTGTAATTTTATGTGTAATTGTGTTTTGATTGTTCGAGGGAGTCCATGTTTTAGCTTAAAGTATATACTAGTTCACACTGTTCTGCAGAGCCCTCATTTCTAAATTTATAGGAAATCTCCACCATCAAGTTATCACATGATCAATAGTTTTTTAATGGATGAGGAGTTTGTTGCCATGTCTTGAGGGTGCAAGAATTATCTGAAAAATCCATTTTTTGTTTGGTTGTGACAGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAAGCAGGAGAATTCTATATCAAATCAATGATTGAATCAGATCGGTCAGCTTCTAGTTCACACCCTACCCAGCCTTGATTATTGCTACCATTTCTATTGGTCATTTGTCTCCAACCATCCCAACATAATTAACCACGTTGCATGTATGTTGACGTGTTGCCAAATTGCACTTTTGTTCCGTCATTTGTCAATTTTCTGTGCTCCTGATCCGCTGCAGATGCCATTGAATCTGCTTTGCTATGTTATATGATATGTCCCCTTCCTCCTGTTCTTGCCTATCAGCATGGGAATATTGCTATTCTTGTAATGTTGAAATTGGACTTTCATTCATTTGCTATTTGTATAGGTTTTTACTTCTTTACCATTTTGCTCGTTCATTCAAATGCTTCACTTATTGGATTCTAATGAAAAAGCAGGTTCAACTGTTAACGAGTTGACATAAAGGCTCTGCTCATTTTCTTCTCTAGCCCTCATCCGGTGGTGGGAAAAGCTGTGAAGTGCTCTGTTGAGTGGTAAAATATTTTTGTATAGAAGGAAGTTAAAAAGGAAAGTCAGGTGAAAACATTATACATCTTTTTACGATACCCTGTCTGACTTATAACTTGAACCAATCCCATGCCCCGTATTAAACACGCCATTGACAGTCAGTATCTTCTCCATTGGTACTGGGCGTCGAGCCATTTCCCTGCCAACTCTGCCACAGCTCCTCTCTTCCCCTCTCTGTTTCTGGAGTGTCACGCCCAGGTGCTTTTCTTTCCCACCCAAATTTAGTACCCTCAACCCTTAATTCAAATTTTCAAATTCAATTATCTCACTACATTTAGCTTCCATCTCCATTGCTTCTCCACATTCACCACTCTATGGGTTGTTGCTGCAGCAGAATCTTGGAAACAGGTCAGCCTCATATCCTAGACAAGGATCGTGGTTCAGCGAAAACTCCGTCACCGGGATGCGCCATCACCGATGTTGGAAACATCCCTGGCACTGGAACCCCGGAAGAGGAAACCGTCAAAGAAGTCCTCTCAGAGACTCCCATTGCCAAGCCATGTAACATACAGCAAACATCCCCAAAGAACAAGTCTTCCGAGCTGAAAGTAAAATCAAGTGAAATGGATGGTTCACTCAACAAAGTGGAGGAAGGTACACTTTCAGTTTCAGAGGTATCTCAGGTTACAGAATGGTGCAGCAATATGAGTGAAAGCGTGTCAATGGCGACCACCATTTCGGAGCAAAGAGAAGGGGACGAAGCATCAAGTAAACAGAGCAGAGAAATGGGTCGGAATCCAAAACCGAAAATTCGAAGAAAGCGTCCATATTCCGGCGACCCATCATACCGAAGAGAACAGAGAGACAAATGTGCAACAAAGAGACCTGCTGAACTGTTATCAGAGAAGAAGTCGCGTGTTACTTGCAGATACACACATGGAACGACAGAATCAAGAGAGGCGAGGACCAGGAAACTGAATGGAGGGCAAGAACAAAAATCTGGAGTCAACCATGGCCGCCGTTCGAGGTCACCAGCTACTCGAACAGTTAGAGAAACGAATAAGACAGGGAATATGAAAAGCAGTGCCTTGAAAGTGACTGGACAAGCCGGTGAGCAGCCAGAGGCAGTGACCACCGAGAAAAGAGACGAAGGAAAGGTGGATAAGGCAATGGATGGTTCAGCAACTCAGCCCCCAAATGAATCCATTGAAAACCCTCTTGTCTCACTTGAATGTTTCATCTTTCTGTAGATTTTAGAACATATTAGTGTATTGGGTAGGAGTTGGTTGGCTTGTATGTCAAAATTCTGGAAATGTGTTGTATACAAGACTTGGGTAGTGGTTGCTGTAGTTATTTGATTGCTTGTTGTTCACTTGGACCGCCCCTTTTTTTCCAAATCTTAAGTGGTATCATGGTTGTTTAGACTCTAAATACAGGAAGAAATTAGTATTTCTCGTGTTTTGAGATTCCAACTT

mRNA sequence

ATGGGATTTGGCGGGAAAACGCTTTACCAACGAACGTCCTTCATCTGTCCTGAGCTTCTCTACGGGGTCGCCAATCTCACGATGAACAGCCATGGCAGAAGCTTAGCTCGACTCCTAGAATCCGTTGGCAGATCCTTCTCTTCTTCATCTTCCTCAACCGCCGCCTCTGCAACCTCCTCCCAATTCTCTCGAACTTTGGCCGGTCTCCGTGAACGACTCGCCGTTGAGACCCCCTCACTTTCCGACTTCCTTGACCTGCAATCTTCTGAGTCGTATTCGGTGGAGGTTGGTACGAAGAAGAAGCCACTTCCCAAGCCTAAATGGATGAAGGAATCCGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAACTTCATACGGTTTGTGAGGAGGCTAGGTGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATACTTGGTGACACTTGTACCCGTGGTTGCAGGCTATCCAGGTTCTGTAACGTCAAAACATCACGAACTCCGCCTCCACCAGACCCAAATGAGCCGAAAAATGTTGCTGAGGCAATCGCATCATGGGGCTTGGATTATGTGGTCATCACTAGTGTGGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACCCCGGCTGTGTAGAAAATGTTGCAAAGTCAGGCCTCGACGTCTTTGCTCATAATATAGAGACAGTTGAGGAGCTTCAGGGAGCTGTTAGGGATCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCCCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAGACACCGGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTACATGAGACCTTCAAAGCGCCATATGCCTGTATCAGAATACATCACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAAGCAGGAGAATTCTATATCAAATCAATGATTGAATCAGATCGGTCAGCTTCTAGTTCACACCCTACCCAGCCTTGATTATTGCTACCATTTCTATTGGTCATTTGTCTCCAACCATCCCAACATAATTAACCACGTTGCATGTATGTTGACGTGTTGCCAAATTGCACTTTTGTTCCGTCATTTGTCAATTTTCTGTGCTCCTGATCCGCTGCAGATGCCATTGAATCTGCTTTGCTATGTTATATGATATGTCCCCTTCCTCCTGTTCTTGCCTATCAGCATGGGAATATTGCTATTCTTGTAATGTTGAAATTGGACTTTCATTCATTTGCTATTTGTATAGGTTTTTACTTCTTTACCATTTTGCTCGTTCATTCAAATGCTTCACTTATTGGATTCTAATGAAAAAGCAGGTTCAACTGTTAACGAGTTGACATAAAGGCTCTGCTCATTTTCTTCTCTAGCCCTCATCCGGTGGTGGGAAAAGCTGTGAAGTGCTCTGTTGAGTGGTAAAATATTTTTGTATAGAAGGAAGTTAAAAAGGAAAGTCAGGTGAAAACATTATACATCTTTTTACGATACCCTGTCTGACTTATAACTTGAACCAATCCCATGCCCCGTATTAAACACGCCATTGACAGTCAGTATCTTCTCCATTGGTACTGGGCGTCGAGCCATTTCCCTGCCAACTCTGCCACAGCTCCTCTCTTCCCCTCTCTGTTTCTGGAGTGTCACGCCCAGAATCTTGGAAACAGGTCAGCCTCATATCCTAGACAAGGATCGTGGTTCAGCGAAAACTCCGTCACCGGGATGCGCCATCACCGATGTTGGAAACATCCCTGGCACTGGAACCCCGGAAGAGGAAACCGTCAAAGAAGTCCTCTCAGAGACTCCCATTGCCAAGCCATGTAACATACAGCAAACATCCCCAAAGAACAAGTCTTCCGAGCTGAAAGTAAAATCAAGTGAAATGGATGGTTCACTCAACAAAGTGGAGGAAGGTACACTTTCAGTTTCAGAGGTATCTCAGGTTACAGAATGGTGCAGCAATATGAGTGAAAGCGTGTCAATGGCGACCACCATTTCGGAGCAAAGAGAAGGGGACGAAGCATCAAGTAAACAGAGCAGAGAAATGGGTCGGAATCCAAAACCGAAAATTCGAAGAAAGCGTCCATATTCCGGCGACCCATCATACCGAAGAGAACAGAGAGACAAATGTGCAACAAAGAGACCTGCTGAACTGTTATCAGAGAAGAAGTCGCGTGTTACTTGCAGATACACACATGGAACGACAGAATCAAGAGAGGCGAGGACCAGGAAACTGAATGGAGGGCAAGAACAAAAATCTGGAGTCAACCATGGCCGCCGTTCGAGGTCACCAGCTACTCGAACAGTTAGAGAAACGAATAAGACAGGGAATATGAAAAGCAGTGCCTTGAAAGTGACTGGACAAGCCGGTGAGCAGCCAGAGGCAGTGACCACCGAGAAAAGAGACGAAGGAAAGGTGGATAAGGCAATGGATGGTTCAGCAACTCAGCCCCCAAATGAATCCATTGAAAACCCTCTTGTCTCACTTGAATGTTTCATCTTTCTGTAGATTTTAGAACATATTAGTGTATTGGGTAGGAGTTGGTTGGCTTGTATGTCAAAATTCTGGAAATGTGTTGTATACAAGACTTGGGTAGTGGTTGCTGTAGTTATTTGATTGCTTGTTGTTCACTTGGACCGCCCCTTTTTTTCCAAATCTTAAGTGGTATCATGGTTGTTTAGACTCTAAATACAGGAAGAAATTAGTATTTCTCGTGTTTTGAGATTCCAACTT

Coding sequence (CDS)

ATGGGATTTGGCGGGAAAACGCTTTACCAACGAACGTCCTTCATCTGTCCTGAGCTTCTCTACGGGGTCGCCAATCTCACGATGAACAGCCATGGCAGAAGCTTAGCTCGACTCCTAGAATCCGTTGGCAGATCCTTCTCTTCTTCATCTTCCTCAACCGCCGCCTCTGCAACCTCCTCCCAATTCTCTCGAACTTTGGCCGGTCTCCGTGAACGACTCGCCGTTGAGACCCCCTCACTTTCCGACTTCCTTGACCTGCAATCTTCTGAGTCGTATTCGGTGGAGGTTGGTACGAAGAAGAAGCCACTTCCCAAGCCTAAATGGATGAAGGAATCCGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAACTTCATACGGTTTGTGAGGAGGCTAGGTGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATACTTGGTGACACTTGTACCCGTGGTTGCAGGCTATCCAGGTTCTGTAACGTCAAAACATCACGAACTCCGCCTCCACCAGACCCAAATGAGCCGAAAAATGTTGCTGAGGCAATCGCATCATGGGGCTTGGATTATGTGGTCATCACTAGTGTGGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACCCCGGCTGTGTAGAAAATGTTGCAAAGTCAGGCCTCGACGTCTTTGCTCATAATATAGAGACAGTTGAGGAGCTTCAGGGAGCTGTTAGGGATCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCCCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAGACACCGGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTACATGAGACCTTCAAAGCGCCATATGCCTGTATCAGAATACATCACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAAGCAGGAGAATTCTATATCAAATCAATGATTGAATCAGATCGGTCAGCTTCTAGTTCACACCCTACCCAGCCTTGA

Protein sequence

MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP
Homology
BLAST of Carg19221 vs. NCBI nr
Match: KAG7011959.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 816.6 bits (2108), Expect = 9.6e-233
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60
           MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS
Sbjct: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60

Query: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120
           QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV
Sbjct: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120

Query: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCNVKT 180
           QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCNVKT
Sbjct: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCNVKT 180

Query: 181 SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML 240
           SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML
Sbjct: 181 SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML 240

Query: 241 IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF 300
           IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF
Sbjct: 241 IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF 300

Query: 301 APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA 360
           APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA
Sbjct: 301 APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA 360

Query: 361 FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 412
           FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 411

BLAST of Carg19221 vs. NCBI nr
Match: KAG6572348.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 807.7 bits (2085), Expect = 4.5e-230
Identity = 409/411 (99.51%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60
           MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS
Sbjct: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60

Query: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120
           QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV
Sbjct: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120

Query: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCNVKT 180
           QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRL  FCNVKT
Sbjct: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLV-FCNVKT 180

Query: 181 SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML 240
           SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML
Sbjct: 181 SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML 240

Query: 241 IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF 300
           IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF
Sbjct: 241 IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF 300

Query: 301 APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA 360
           APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA
Sbjct: 301 APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA 360

Query: 361 FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 412
           FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 410

BLAST of Carg19221 vs. NCBI nr
Match: XP_022952737.1 (lipoyl synthase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 739.2 bits (1907), Expect = 1.9e-209
Identity = 376/384 (97.92%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLESVGR FSSSSSS AASATSSQF RTLAGLRERLAVETPSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESVGRFFSSSSSSAAASATSSQFPRTLAGLRERLAVETPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCT   R+SRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCT---RVSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME
Sbjct: 241 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSSHPTQP 412
           AGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 AGEFYIKSMIESDRSASSSHPTQP 381

BLAST of Carg19221 vs. NCBI nr
Match: XP_023554514.1 (lipoyl synthase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 730.7 bits (1885), Expect = 6.9e-207
Identity = 370/384 (96.35%), Postives = 374/384 (97.40%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLES+GRSFSSSSSSTAAS T SQF RTL GLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESIGRSFSSSSSSTAASETFSQFPRTLTGLRERLAAESPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPL KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLSKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFK SLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME
Sbjct: 241 HNIETVEELQGAVRDHRANFKPSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDV+TFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVITFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSSHPTQP 412
           AGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 AGEFYIKSMIESDRSASSSHPTQP 381

BLAST of Carg19221 vs. NCBI nr
Match: XP_023554516.1 (lipoyl synthase 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712.2 bits (1837), Expect = 2.5e-201
Identity = 360/374 (96.26%), Postives = 364/374 (97.33%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLES+GRSFSSSSSSTAAS T SQF RTL GLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESIGRSFSSSSSSTAASETFSQFPRTLTGLRERLAAESPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPL KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLSKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFK SLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME
Sbjct: 241 HNIETVEELQGAVRDHRANFKPSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDV+TFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVITFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDR 402
           AGEFYIKSMIESDR
Sbjct: 361 AGEFYIKSMIESDR 371

BLAST of Carg19221 vs. ExPASy Swiss-Prot
Match: B9H5L9 (Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.2e-180
Identity = 317/361 (87.81%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 50  SSSTAASATSS--QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPK 109
           SSST  S+T    QFS+TLAGLR RLAVE+P+LSDF+ LQS+ +YSVEVGTKKKPLPKPK
Sbjct: 26  SSSTVESSTKQPPQFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTKKKPLPKPK 85

Query: 110 WMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCT 169
           WM+E++PGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECWSGGETGTATATIMILGDTCT
Sbjct: 86  WMREAIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCT 145

Query: 170 RGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAE 229
           RGC   RFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVITSVDRDDL DQGSGHFAE
Sbjct: 146 RGC---RFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLADQGSGHFAE 205

Query: 230 TVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANF 289
           TV KLK LKPNMLIEALVPDFRGD GCVE VAKSGLDVFAHNIETVEELQ +VRDHRANF
Sbjct: 206 TVHKLKTLKPNMLIEALVPDFRGDRGCVEKVAKSGLDVFAHNIETVEELQSSVRDHRANF 265

Query: 290 KQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSK 349
           KQSLDVL+MAKE+AP GTLTKTSIMLGCGE P+QVV+TMEKVRAAGVDVMTFGQYMRPSK
Sbjct: 266 KQSLDVLMMAKEYAPPGTLTKTSIMLGCGEAPEQVVKTMEKVRAAGVDVMTFGQYMRPSK 325

Query: 350 RHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSH 409
           RHMPVSEYITP+AFEKY+ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMIESDRS SS  
Sbjct: 326 RHMPVSEYITPDAFEKYKTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSVSSQL 383

BLAST of Carg19221 vs. ExPASy Swiss-Prot
Match: Q3LSN5 (Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 9.8e-180
Identity = 315/378 (83.33%), Postives = 344/378 (91.01%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M S  R++A  L    +  SSSS++T  ++  S+  + LA LR RLA+E+PSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           S  +YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDP+EP NVAEAIASWGLDYV
Sbjct: 122 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYV 181

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHF ETVQKLK LKP+MLIEALVPDFRG+  CVE V+KSGLDVFA
Sbjct: 182 VITSVDRDDLPDQGSGHFTETVQKLKALKPSMLIEALVPDFRGNAECVEKVSKSGLDVFA 241

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLGCGETPDQ+V+TME
Sbjct: 242 HNIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTME 301

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQ+MRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYK
Sbjct: 302 KVRAAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYK 361

Query: 388 AGEFYIKSMIESDRSASS 406
           AGEFYIKSMI+SDR+ASS
Sbjct: 362 AGEFYIKSMIDSDRAASS 376

BLAST of Carg19221 vs. ExPASy Swiss-Prot
Match: Q3LSN4 (Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.3e-179
Identity = 315/378 (83.33%), Postives = 343/378 (90.74%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M S  R++A  L    +  SSSS++T  ++  S+  + LA LR RLA+E+PSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           S  +YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDP+EP NVAEAIASWGLDYV
Sbjct: 122 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYV 181

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHF ETVQKLK LKP+ LIEALVPDFRG+  CVE V+KSGLDVFA
Sbjct: 182 VITSVDRDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAECVEKVSKSGLDVFA 241

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLGCGETPDQ+V+TME
Sbjct: 242 HNIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTME 301

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYK
Sbjct: 302 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYK 361

Query: 388 AGEFYIKSMIESDRSASS 406
           AGEFYIKSMI+SDR+ASS
Sbjct: 362 AGEFYIKSMIDSDRAASS 376

BLAST of Carg19221 vs. ExPASy Swiss-Prot
Match: B7FM45 (Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 1.9e-175
Identity = 307/378 (81.22%), Postives = 342/378 (90.48%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M S  R++A+ L+S  + FS ++++T  + +SS+F + L  LR RLA E+PSLSDF+ L+
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTATTTTTVSSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           S+ +YSVEVGTKK PLPKPKWMKES+PGG KYVQIKKKLRELKLHTVCEEA+CPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDP+EP NVAEAIASWGLDYV
Sbjct: 122 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYV 181

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSV RDDLPDQGS HF ETVQKLK+LKP++LIEALVPDFRG+  CVE V+KSGLDVFA
Sbjct: 182 VITSVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFA 241

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQ AVRDHRANF QSLDVL MAK++AP+GTLTKTSIMLGCGETPDQ+V+TME
Sbjct: 242 HNIETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTME 301

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYK
Sbjct: 302 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYK 361

Query: 388 AGEFYIKSMIESDRSASS 406
           AGEFYIKSMI+SDR+ SS
Sbjct: 362 AGEFYIKSMIDSDRAVSS 376

BLAST of Carg19221 vs. ExPASy Swiss-Prot
Match: A5CB81 (Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 9.5e-175
Identity = 305/354 (86.16%), Postives = 328/354 (92.66%), Query Frame = 0

Query: 58  TSSQFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGE 117
           T+ +  +TLA LR RLAVE+PSLSDF+ LQ+S+ YSVEVGTKKKPL KPKWMKES+PGG 
Sbjct: 38  TNPEPPQTLASLRHRLAVESPSLSDFVRLQTSDDYSVEVGTKKKPLSKPKWMKESIPGGA 97

Query: 118 KYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCN 177
           KY QIKKKLR+L LHTVCEEARCPN+GECWSGGETGTATATIMILGDTCTRGC   RFCN
Sbjct: 98  KYTQIKKKLRQLNLHTVCEEARCPNMGECWSGGETGTATATIMILGDTCTRGC---RFCN 157

Query: 178 VKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKP 237
           VKTSRTPPPPDP+EP  VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLK+LKP
Sbjct: 158 VKTSRTPPPPDPDEPSKVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKILKP 217

Query: 238 NMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMA 297
           NMLIEALVPDFRGDPGCVE V+KSGLDVFAHNIETVEELQ AVRDHRANFKQSL+VL +A
Sbjct: 218 NMLIEALVPDFRGDPGCVEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLEVLKLA 277

Query: 298 KEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYIT 357
           KE+A +GTLTKTSIMLGCGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYIT
Sbjct: 278 KEYADAGTLTKTSIMLGCGETPDQVVRTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYIT 337

Query: 358 PEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSAS--SSHPT 410
           PEAFEKY+ LGM MGFRYVASGPMVRSSYKAGEFYIKSMI++DR+ S  SS P+
Sbjct: 338 PEAFEKYRILGMDMGFRYVASGPMVRSSYKAGEFYIKSMIDADRAMSWASSSPS 388

BLAST of Carg19221 vs. ExPASy TrEMBL
Match: A0A6J1GL74 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455343 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 9.4e-210
Identity = 376/384 (97.92%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLESVGR FSSSSSS AASATSSQF RTLAGLRERLAVETPSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESVGRFFSSSSSSAAASATSSQFPRTLAGLRERLAVETPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCT   R+SRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCT---RVSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME
Sbjct: 241 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSSHPTQP 412
           AGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 AGEFYIKSMIESDRSASSSHPTQP 381

BLAST of Carg19221 vs. ExPASy TrEMBL
Match: A0A6J1I045 (Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111468222 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.0e-200
Identity = 362/384 (94.27%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLESVGRSFSSSSSS           RTLAGLRERLA ETPSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESVGRSFSSSSSSP---------XRTLAGLRERLAAETPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPL KPKW+KESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLSKPKWLKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGCRL   CNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRL---CNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTL KTSIMLGCGETPDQVVQ+ME
Sbjct: 241 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLAKTSIMLGCGETPDQVVQSME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMR SKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGP+VRSSYK
Sbjct: 301 KVRAAGVDVMTFGQYMRTSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPIVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSSHPTQP 412
           AGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 AGEFYIKSMIESDRSASSSHPTQP 372

BLAST of Carg19221 vs. ExPASy TrEMBL
Match: A0A5A7SKJ1 (Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.3e-200
Identity = 358/383 (93.47%), Postives = 370/383 (96.61%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MN   +SLAR+L+SV RSFSSSSSST+ASATSSQF RTLAGLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVE VA+SGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTME
Sbjct: 241 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSSHPTQ 411
           AGEFYIKSMIESDR+AS+S PTQ
Sbjct: 361 AGEFYIKSMIESDRAASNSQPTQ 380

BLAST of Carg19221 vs. ExPASy TrEMBL
Match: A0A1S4E162 (Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.3e-200
Identity = 358/383 (93.47%), Postives = 370/383 (96.61%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MN   +SLAR+L+SV RSFSSSSSST+ASATSSQF RTLAGLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVE VA+SGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTME
Sbjct: 241 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSSHPTQ 411
           AGEFYIKSMIESDR+AS+S PTQ
Sbjct: 361 AGEFYIKSMIESDRAASNSQPTQ 380

BLAST of Carg19221 vs. ExPASy TrEMBL
Match: A0A6J1ERM2 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 9.8e-199
Identity = 356/383 (92.95%), Postives = 367/383 (95.82%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MN+   SLAR+ ESVGRS S SSSST+ASATSSQ  RTLAGLRERLA E+P+LSDF+ L+
Sbjct: 1   MNARRHSLARIFESVGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDPNEP+NVAEAIASWGLDYV
Sbjct: 121 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPG VE VAKSGLDVFA
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME
Sbjct: 241 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSSHPTQ 411
           AGEFYIKSMIESDR+ASSSHPTQ
Sbjct: 361 AGEFYIKSMIESDRAASSSHPTQ 380

BLAST of Carg19221 vs. TAIR 10
Match: AT2G20860.1 (lipoic acid synthase 1 )

HSP 1 Score: 607.4 bits (1565), Expect = 8.2e-174
Identity = 307/379 (81.00%), Postives = 336/379 (88.65%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M+S    L R L    R FSSSS+ T  + T S   ++L  LR RLA E+PSL+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFI--- 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
             ++YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEA+CPNLGECW
Sbjct: 61  HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYV 207
           SGGETGTATATIMILGDTCTRGC   RFCNVKTSRTPPPPDPNEP NVAEAIASWG+DYV
Sbjct: 121 SGGETGTATATIMILGDTCTRGC---RFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYV 180

Query: 208 VITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFA 267
           VITSVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVE V+KSGLDV A
Sbjct: 181 VITSVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLA 240

Query: 268 HNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTME 327
           HNIETVEELQ  VRDHRANFKQSLDVL MAKE+AP+GTLTKTS+MLGCGETPDQVV+TME
Sbjct: 241 HNIETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTME 300

Query: 328 KVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYK 387
           KVRAAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ LGM+MGFRYVASGPMVRSSYK
Sbjct: 301 KVRAAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYK 360

Query: 388 AGEFYIKSMIESDRSASSS 407
           AGE+YIKSMIE+DR AS S
Sbjct: 361 AGEYYIKSMIEADRVASPS 371

BLAST of Carg19221 vs. TAIR 10
Match: AT5G08415.1 (Radical SAM superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.4e-104
Identity = 177/302 (58.61%), Postives = 232/302 (76.82%), Query Frame = 0

Query: 105 KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGD 164
           KP W+++  P GE++ ++K+ L  L L+TVCEEA+CPN+GECW+GG  G ATATIM+LGD
Sbjct: 97  KPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGD 156

Query: 165 TCTRGCRLSRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGH 224
           TCTRGC   RFC VKTSR PPPPDP EP+N A+AIASWG+DY+VITSVDRDD+PD GSGH
Sbjct: 157 TCTRGC---RFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGH 216

Query: 225 FAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHR 284
           FA+TV+ +K  KP+++IE L  DFRGD   V+ +  SGLDVFAHN+ETV+ LQ  VRD R
Sbjct: 217 FAQTVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPR 276

Query: 285 ANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMR 344
           A ++QS+ VL  AK   P G +TKTSIMLG GET +++ + M  +RA  VD++T GQY++
Sbjct: 277 AGYEQSMSVLKHAKISKP-GMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQ 336

Query: 345 PSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSAS 404
           P+  H+ V EY+TPE F+ ++  G  +GFRYVASGP+VRSSY+AGE ++K+M++   S S
Sbjct: 337 PTPLHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESYSKS 394

Query: 405 SS 407
            S
Sbjct: 397 LS 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011959.19.6e-233100.00Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6572348.14.5e-23099.51Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022952737.11.9e-20997.92lipoyl synthase 2, mitochondrial-like [Cucurbita moschata][more]
XP_023554514.16.9e-20796.35lipoyl synthase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023554516.12.5e-20196.26lipoyl synthase 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
B9H5L91.2e-18087.81Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1[more]
Q3LSN59.8e-18083.33Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1[more]
Q3LSN41.3e-17983.33Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1[more]
B7FM451.9e-17581.22Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1[more]
A5CB819.5e-17586.16Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GL749.4e-21097.92Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455343 PE=... [more]
A0A6J1I0451.0e-20094.27Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111468222 PE=3 ... [more]
A0A5A7SKJ12.3e-20093.47Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE... [more]
A0A1S4E1622.3e-20093.47Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1[more]
A0A6J1ERM29.8e-19992.95Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=... [more]
Match NameE-valueIdentityDescription
AT2G20860.18.2e-17481.00lipoic acid synthase 1 [more]
AT5G08415.12.4e-10458.61Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 156..366
e-value: 1.5E-4
score: 28.2
IPR003698Lipoyl synthaseTIGRFAMTIGR00510TIGR00510coord: 99..396
e-value: 4.2E-119
score: 395.2
IPR003698Lipoyl synthaseSFLDSFLDF00271lipoyl_synthasecoord: 97..396
e-value: 0.0
score: 427.5
IPR003698Lipoyl synthasePANTHERPTHR10949LIPOYL SYNTHASEcoord: 59..403
IPR003698Lipoyl synthaseHAMAPMF_00206Lipoyl_synthcoord: 105..402
score: 128.743683
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 165..328
e-value: 9.9E-9
score: 35.8
IPR007197Radical SAMSFLDSFLDS00029Radical_SAMcoord: 97..396
e-value: 0.0
score: 427.5
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 151..374
score: 27.294479
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 149..378
e-value: 9.8E-11
score: 43.2
IPR031691Lipoyl synthase, N-terminalPFAMPF16881LIAS_Ncoord: 54..140
e-value: 2.0E-17
score: 63.6
NoneNo IPR availablePANTHERPTHR10949:SF0LIPOYL SYNTHASE, MITOCHONDRIALcoord: 59..403
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 121..400
IPR027527Lipoyl synthase, mitochondrialHAMAPMF_03128Lipoyl_synth_plantMcoord: 47..405
score: 61.057121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19221-RACarg19221-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009107 lipoate biosynthetic process
biological_process GO:0009249 protein lipoylation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016992 lipoate synthase activity
molecular_function GO:0102552 lipoyl synthase activity (acting on glycine-cleavage complex H protein
molecular_function GO:0102553 lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding