Carg19171 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19171
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ThiF domain-containing protein
Location	Carg_Chr19: 8181766 .. 8182140 (-)
RNA-Seq Expression	Carg19171
Synteny	Carg19171

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCGAAGATCCAGAAGATCCGGGAACTCTCATTCCGAGACGTTTCACAAGTATCTAAAGCCTGGTGCGCTAGCACAAATCCGGAATTCGCGGATGATCGCCCGATTCAATCGGGTTAATTTGGTCTCTCAGATCTACTCCACTCGTTCCTCATCGCTATCGCCTTCTACTGCGCCGATTGGCTCCGGTCTGCTTCAGGTGAACATTGCTGATGCTTCTCCTTGCTTTGCTGGGAGGGTGTATGGTCCCCTCTGCTTGCAGAGAAAAAAACTTTTGGCTGCTAAGTCTTCTTTGTGGTTCCTCGCTTCTAGCCCGACCAATCTCACACCCGATTCTCCGCCTGATCCGGTTATTGGAGTCTTAGGGGGGTGA

mRNA sequence

ATGAATCGAAGATCCAGAAGATCCGGGAACTCTCATTCCGAGACGTTTCACAAGTATCTAAAGCCTGGTGCGCTAGCACAAATCCGGAATTCGCGGATGATCGCCCGATTCAATCGGGTTAATTTGGTCTCTCAGATCTACTCCACTCGTTCCTCATCGCTATCGCCTTCTACTGCGCCGATTGGCTCCGGTCTGCTTCAGGTGAACATTGCTGATGCTTCTCCTTGCTTTGCTGGGAGGGTGTATGGTCCCCTCTGCTTGCAGAGAAAAAAACTTTTGGCTGCTAAGTCTTCTTTGTGGTTCCTCGCTTCTAGCCCGACCAATCTCACACCCGATTCTCCGCCTGATCCGGTTATTGGAGTCTTAGGGGGGTGA

Coding sequence (CDS)

ATGAATCGAAGATCCAGAAGATCCGGGAACTCTCATTCCGAGACGTTTCACAAGTATCTAAAGCCTGGTGCGCTAGCACAAATCCGGAATTCGCGGATGATCGCCCGATTCAATCGGGTTAATTTGGTCTCTCAGATCTACTCCACTCGTTCCTCATCGCTATCGCCTTCTACTGCGCCGATTGGCTCCGGTCTGCTTCAGGTGAACATTGCTGATGCTTCTCCTTGCTTTGCTGGGAGGGTGTATGGTCCCCTCTGCTTGCAGAGAAAAAAACTTTTGGCTGCTAAGTCTTCTTTGTGGTTCCTCGCTTCTAGCCCGACCAATCTCACACCCGATTCTCCGCCTGATCCGGTTATTGGAGTCTTAGGGGGGTGA

Protein sequence

MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAPIGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIGVLGG

Homology

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BLAST of Carg19171 vs. NCBI nr
Match: XP_022952539.1 (uncharacterized protein LOC111455201 [Cucurbita moschata] >KAG6572294.1 hypothetical protein SDJN03_29022, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011910.1 hypothetical protein SDJN02_26817, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 244.6 bits (623), Expect = 4.5e-61
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0


Query: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60
           MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP
Sbjct: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60

Query: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120
           IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG
Sbjct: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120

Query: 121 VLGG 125
           VLGG
Sbjct: 121 VLGG 124

[Close]

BLAST of Carg19171 vs. NCBI nr
Match: XP_023554641.1 (uncharacterized protein LOC111811831 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.8 bits (608), Expect = 2.5e-59
Identity = 120/124 (96.77%), Postives = 122/124 (98.39%), Query Frame = 0


Query: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60
           MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPST P
Sbjct: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTTP 60

Query: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120
           +GS +LQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG
Sbjct: 61  MGSSMLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120

Query: 121 VLGG 125
           VLGG
Sbjct: 121 VLGG 124

[Close]

BLAST of Carg19171 vs. NCBI nr
Match: XP_022969297.1 (uncharacterized protein LOC111468346 [Cucurbita maxima])

HSP 1 Score: 232.6 bits (592), Expect = 1.8e-57
Identity = 118/124 (95.16%), Postives = 120/124 (96.77%), Query Frame = 0


Query: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60
           MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTR SSLS ST P
Sbjct: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRPSSLSSSTTP 60

Query: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120
           +GSGLLQVN+ADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASS TNLTPDSPPDPVIG
Sbjct: 61  MGSGLLQVNVADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSLTNLTPDSPPDPVIG 120

Query: 121 VLGG 125
           VLGG
Sbjct: 121 VLGG 124

[Close]

BLAST of Carg19171 vs. NCBI nr
Match: XP_023530330.1 (uncharacterized protein LOC111792933 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 215.7 bits (548), Expect = 2.3e-52
Identity = 115/127 (90.55%), Postives = 116/127 (91.34%), Query Frame = 0


Query: 1   MNRRSR---RSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPS 60
           MNRRSR   RS  SHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSP 
Sbjct: 1   MNRRSRAFSRSSKSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPP 60

Query: 61  TAPIGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDP 120
           TAPI S LLQVNI DASPCFAGRVYGPLCLQRKKLLAAK SLWFL+SSPTNLTPDSPPDP
Sbjct: 61  TAPIDSALLQVNIVDASPCFAGRVYGPLCLQRKKLLAAK-SLWFLSSSPTNLTPDSPPDP 120

Query: 121 VIGVLGG 125
           VI VLGG
Sbjct: 121 VIEVLGG 126

[Close]

BLAST of Carg19171 vs. NCBI nr
Match: KAG7022864.1 (hypothetical protein SDJN02_16600, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 215.3 bits (547), Expect = 2.9e-52
Identity = 114/127 (89.76%), Postives = 116/127 (91.34%), Query Frame = 0


Query: 1   MNRRSR---RSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPS 60
           MNRRSR   RS  SHSETFHKYLKPGALAQIRNSRMIARFNRVNL+SQIYSTRSSSLSP 
Sbjct: 1   MNRRSRAFPRSSKSHSETFHKYLKPGALAQIRNSRMIARFNRVNLISQIYSTRSSSLSPP 60

Query: 61  TAPIGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDP 120
           TAPI S LLQVNI DASPCFAGRVYGPLCLQRKKLLAAK SLWFL+SSPTNLTPDSPPDP
Sbjct: 61  TAPIDSALLQVNIVDASPCFAGRVYGPLCLQRKKLLAAK-SLWFLSSSPTNLTPDSPPDP 120

Query: 121 VIGVLGG 125
           VI VLGG
Sbjct: 121 VIEVLGG 126

[Close]

BLAST of Carg19171 vs. ExPASy TrEMBL
Match: A0A6J1GLZ8 (uncharacterized protein LOC111455201 OS=Cucurbita moschata OX=3662 GN=LOC111455201 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.2e-61
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0


Query: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60
           MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP
Sbjct: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60

Query: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120
           IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG
Sbjct: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120

Query: 121 VLGG 125
           VLGG
Sbjct: 121 VLGG 124

[Close]

BLAST of Carg19171 vs. ExPASy TrEMBL
Match: A0A6J1HVZ0 (uncharacterized protein LOC111468346 OS=Cucurbita maxima OX=3661 GN=LOC111468346 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.6e-58
Identity = 118/124 (95.16%), Postives = 120/124 (96.77%), Query Frame = 0


Query: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60
           MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTR SSLS ST P
Sbjct: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRPSSLSSSTTP 60

Query: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120
           +GSGLLQVN+ADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASS TNLTPDSPPDPVIG
Sbjct: 61  MGSGLLQVNVADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSLTNLTPDSPPDPVIG 120

Query: 121 VLGG 125
           VLGG
Sbjct: 121 VLGG 124

[Close]

BLAST of Carg19171 vs. ExPASy TrEMBL
Match: A0A6J1EXJ2 (uncharacterized protein LOC111437092 OS=Cucurbita moschata OX=3662 GN=LOC111437092 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.4e-52
Identity = 114/127 (89.76%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MNRRSR---RSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPS 60
           MNRRSR   RS  SHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTR SSLSP 
Sbjct: 1   MNRRSRAFPRSSKSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRFSSLSPP 60

Query: 61  TAPIGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDP 120
           TAPI S LLQVNI DASPCFAGRVYGPLCLQRKKLLAAK SLWFL+SSPTNLTPDSPPDP
Sbjct: 61  TAPIDSALLQVNIVDASPCFAGRVYGPLCLQRKKLLAAK-SLWFLSSSPTNLTPDSPPDP 120

Query: 121 VIGVLGG 125
           VI VLGG
Sbjct: 121 VIEVLGG 126

[Close]

BLAST of Carg19171 vs. ExPASy TrEMBL
Match: A0A6J1JMJ5 (uncharacterized protein LOC111486563 OS=Cucurbita maxima OX=3661 GN=LOC111486563 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.6e-51
Identity = 114/127 (89.76%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MNRRSR---RSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPS 60
           MNRRSR   RS  SHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSP 
Sbjct: 1   MNRRSRAFPRSSKSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPP 60

Query: 61  TAPIGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDP 120
           TAPI S LLQVNI DASPCFAGRVYGPLCLQRKKLLAAK SLWFL+SS TNLTPDSPPDP
Sbjct: 61  TAPIDSALLQVNIVDASPCFAGRVYGPLCLQRKKLLAAK-SLWFLSSSLTNLTPDSPPDP 120

Query: 121 VIGVLGG 125
           VI VLGG
Sbjct: 121 VIEVLGG 126

[Close]

BLAST of Carg19171 vs. ExPASy TrEMBL
Match: A0A5D3C9X6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G004250 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-48
Identity = 110/124 (88.71%), Postives = 111/124 (89.52%), Query Frame = 0


Query: 1   MNRRSRRSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAP 60
           MNRRSRRSGNS SETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYST SSSL     P
Sbjct: 1   MNRRSRRSGNSQSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTTSSSL-----P 60

Query: 61  IGSGLLQVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNLTPDSPPDPVIG 120
           I SGLLQVNI DASP FAGRVYGPL LQRKKLLA+K SLWFLASSPTNLTPDSPPDPVI 
Sbjct: 61  IDSGLLQVNIVDASPSFAGRVYGPLSLQRKKLLASK-SLWFLASSPTNLTPDSPPDPVIE 118

Query: 121 VLGG 125
           VLGG
Sbjct: 121 VLGG 118

[Close]

BLAST of Carg19171 vs. TAIR 10
Match: AT5G54970.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G26960.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 59.3 bits (142), Expect = 2.5e-09
Identity = 44/100 (44.00%), Postives = 55/100 (55.00%), Query Frame = 0


Query: 7  RSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAPIGSGLL 66
          +S    S+ FH+YLKPGALAQIRNSR+ AR +  +LVS+      SSLS    PI     
Sbjct: 6  KSPARRSDGFHRYLKPGALAQIRNSRINAR-STTSLVSR------SSLSQLVDPISPSEN 65

Query: 67 QVNIA---------DASPCFAGRVYGPLCLQRKKLLAAKS 98
           V+ A         D  P    ++YGP   QRKKL AA+S
Sbjct: 66 SVDEAAAASRNLTIDQVPHLLRKIYGPYSYQRKKLAAARS 98

[Close]

BLAST of Carg19171 vs. TAIR 10
Match: AT4G26960.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G54970.1); Has 22 Blast hits to 22 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 22; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 55.1 bits (131), Expect = 4.8e-08
Identity = 36/103 (34.95%), Postives = 54/103 (52.43%), Query Frame = 0


Query: 7   RSGNSHSETFHKYLKPGALAQIRNSRMIARFNRVNLVSQIYSTRSSSLSPSTAPIGSGLL 66
           RS    ++ FH+Y+KPGALAQIRN+R+    +   L   +        S S +PI +   
Sbjct: 35  RSPVRRNDGFHRYMKPGALAQIRNARINNARSNFPLTRSLSDPVDPPSSSSESPI-AATP 94

Query: 67  QVNIADASPCFAGRVYGPLCLQRKKLLAAKSSLWFLASSPTNL 110
           Q    D  P    ++YGP  + RKKL  A+S++  L  SP ++
Sbjct: 95  QTITMDQMPHLLSKIYGPYRIGRKKLGPARSAMVKLNPSPNSI 136

The following BLAST results are available for this feature:

BLAST of Carg19171 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952539.1	4.5e-61	100.00	uncharacterized protein LOC111455201 [Cucurbita moschata] >KAG6572294.1 hypothet...	[more]
XP_023554641.1	2.5e-59	96.77	uncharacterized protein LOC111811831 [Cucurbita pepo subsp. pepo]	[more]
XP_022969297.1	1.8e-57	95.16	uncharacterized protein LOC111468346 [Cucurbita maxima]	[more]
XP_023530330.1	2.3e-52	90.55	uncharacterized protein LOC111792933 [Cucurbita pepo subsp. pepo]	[more]
KAG7022864.1	2.9e-52	89.76	hypothetical protein SDJN02_16600, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Carg19171 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg19171 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GLZ8	2.2e-61	100.00	uncharacterized protein LOC111455201 OS=Cucurbita moschata OX=3662 GN=LOC1114552...	[more]
A0A6J1HVZ0	8.6e-58	95.16	uncharacterized protein LOC111468346 OS=Cucurbita maxima OX=3661 GN=LOC111468346...	[more]
A0A6J1EXJ2	5.4e-52	89.76	uncharacterized protein LOC111437092 OS=Cucurbita moschata OX=3662 GN=LOC1114370...	[more]
A0A6J1JMJ5	1.6e-51	89.76	uncharacterized protein LOC111486563 OS=Cucurbita maxima OX=3661 GN=LOC111486563...	[more]
A0A5D3C9X6	1.3e-48	88.71	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Carg19171 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54970.1	2.5e-09	44.00	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT4G26960.1	4.8e-08	34.95	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR35495	OS06G0679600 PROTEIN	coord: 1..123
None	No IPR available	PANTHER	PTHR35495:SF10	SUBFAMILY NOT NAMED	coord: 1..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19171-RA	Carg19171-RA	mRNA