Carg18988 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18988
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionZn-dependent exopeptidases superfamily protein
LocationCarg_Chr18: 9633299 .. 9637088 (-)
RNA-Seq ExpressionCarg18988
SyntenyCarg18988
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAATTTTAAAATCTATGTATATAACAATCAATTGGATGTCTGTGAAGAACCGGACGAAGAAATGGCGGGAATATCTCGCTTGCAAGTGCATCTCCTTCACTCTACTCCTCCCGCTCTCAAGTCGACTTCGCTCCTCTCGATGTTTCCTCGCTTTTCTCGCTCTACTCCGCGTAAAATTTTCACTCCTCGTCTTCTCTGTTCGACTCCTCAGGTTCGTCTCCGTTTTTCTATCTCATTTTGCATGAAAAGTTGTGTGATGTGTTCGTATATTTGTTGTGATATAGAGTTCTTCTTCGGAGGCTGGTTCGAGTTCGAGCAGTGTTGGTGATCTTCTCGATTATCTCAATGAGTCCTGGACTCAGTTTCATTCTACAGGTATTGATTGCTTTTGTATCATCTTTTATCGCTATGTTTTTTTTTCTTTTTTTCTTTTTGGTGTTTCGATTCGAAGAGGAGTTCCTTGCGTATTGACTGGATAATAAATCCTAATTAGCGCTGCGGCTGCTAGTTTTTTGATTCCGTAGAGTGGTTTGAATTGTTTCGAGTCGACGTGTTCGTTTATTCTTATTATTGTTGTGCATTAATGATTCGTCGTTCACTTCTCACCTTAGAGGCCGAAGTTTGGGATTTTTATGGCGAAATCTTAGATACCTTCGATTCAACATTCTGGTGATAGAATTTATGATGCGGATACCTCGGTCAGTGTTTTATATCTTATTTTATTTTATTTTATTTTATTTTTCTCGCAGCCGAATCGAAACGGCATTTAGTTGCTGCTGGTTTTCATTTGCTAAACGAGGATGAAGAGTGGGACTTAAAAACCTGGTGGATGCTACTTTTTTACACGAAATATGTCTTGTTTGGTTGCCTTTTCCATAGGAGAAAAGTGAGTTCTAGAATTTTTGTTCTAATTTTCAGATCTGCAAATCATTCCGCTTCTTTGTCATTCAATTTATGTGGCTTATGAAACTACAGAAGAAATCTGGCTAGTCGGCCACTTGACTTGTTTTGCAAGATTTTGCTCTTGGTCTACTCATCAGTTTGTAGCTTTCCTTGCGTCAAGCTATTGTGCTTTGAGAGCTCTTATTGATTCTTGTGAATCGCCTAGTGACTTAAAGAGTGAACAGGCAGTTCGAATGGTTGCTTTATTTGATATTGAAGAGGTAATAGTTTTATTGTGTACTATATTATGAATGATATGCAGACCGGAAGTCTTGCATCTCTTGGATTGGTCAAAGATATTTTGCATATTTGCTCTCTCTGCCATATAAATTCTTTATAATTTGATCATGTCTATTGCTATGATAAGATAGTACTGCCTATTAATTATAGAACTGAAGATTTTAGGCACCTTTGATCACCAGAAATGCAGTCTATTTCTGTTTCCTTCAGCCATTAGAAAGATGAGTAGAAGTGAATTTTCTAGATAAGCGGATGAATGATCATCGGTCCATACTCCGGTGGTATACTTTCTGCTAGAGGGTAAAGTAATACCTTCCAAGCGCATGAAAACCAAAAGAGATCAGAAGTTCCCCAATCATTTTTATTCCAAGTCTCTATGTTTTGTGAAGAACTAGAAGAATTATGTAGGAGACAGATCTTTATGATAATTTGTAACGGCCTAAGCCCACCGCTAGTAGATATTGTCTGCTTTGGCCCGTTATGTATCGCCGTCAGACTCACGATTTTAAAACGCGTCTACTAGGGAGAGGTTTCCACACCCTTATTAGTAATGCTTCATTTTCCTCTCCAACCGATGTAGGATCTCACATAATTTGTTGCACCACCTAGCCAGGATATAAAATATAGAGACCCTCCAATATGGAAGTATCCTCTCAATATCTTCTAGTTTTCACTCTTATGAGAAAATGAGGGAAAACTATGAGATTCTAAAATCAATCTTCCAACTGCTATGTCTACGTCTCACTACTCTGCACTGTAATCTTAGTAAAAGAGTGCATCCCAAAGAATTATTTCTTATTTGATACATCCAAAACTATGTTCACTTTTTACATATTAGCTTATTCTAAGGTTCCAACTCATCTAGCTCAAATTCTATATTCAATCCCAAGCATCCTTAGCAAGATGTCTGCTGCATCAAGATCCTTCAGATCTTCGGAAGGTCATTGATGAATTAAGGTTCTTTCTGCAAATAAATAATTATCATTTCTCTATTGTTCATGTCAATAATCTCTTGCTTGTCTTTAAGTAACCTAGTATGATCATTACTTGACAGCTGTTTCACACACAAACACACATATATTAGTCGTTAACTTTTCCTATTTAGGTGGGCTCAGGTTCAATTCAGGAAGCTGGTGCACCCACCATGTTTCAGGCCATGAGGCGCATAGCCAGCTGCTTAGGCCAAGGATACGTTGGTGAAGGTGCTGTTGAGCGCGCTTTTAGGCAATCATTTCTCGGTACGTTCAATTCAAAGTAGAAAAGGATTTTTTTTTTTTTTTTTTTCCCTTGAAAGATCAAGTACTATATATTAAACGTGCGAACTAACTCATGATAGGAAAAGTAACTGAAATAGTCGAGTAGCAATCTTAAACTAATTGCGAGTAATATGAACTTGAAAAAGTTGGAGGTGGTCTGATTTAATTTCAATTGCTTTGCATCTTATCGAATTTTTTACTTTCTAATACAGTTATCCTTGAGTCAGAGCGTCGATATTATTGTTGTGCATCCACATAAACACTAGATAGATGTAAACTGGTAAGAAAAATTACATTTTGTGAAAATGCTTGCGTAGTTTATTAGGAATCATGAACCTTCACAATGGTATGATATTGTTCACTTTGAACATAAGCTCTCGTGGTTTTACTTTTAGTTTCCTCAAAAGGTCTCATTCCAATAGAGATAGTATTTCTTACCTAGATCATCCTCTTAATTAGCCAATATAGAACTACTCCTCTTACTAATGTTCGAACATACTTGATTTGTTTACCATAAAGATTAATTTGAACACATCATAGGAAAATGTAGTATGCTTCTGTGACATATCTTAACTTCTAATTTCTTACCTATATAATTGTATTCTAAATAGTTGAACGCGTGTGTCAGAATTTAGTGCAATTTATTTCTAGTTTGAATTAGTTATGGAATATATGATATTCGTAATCTTGGAGAATATATTAGTTAGGAAATATATCTTAGATATTAAATTTGAAGGTATTTTATTTTATTCTCAAGATTTAGTTAGTTTATATTATTTAGTATAGTATATCCTATTCAAAGGTTGTGAATATCAATGAAACTTGAATCTTCCCTTCCAATTCTATTTCTCATTCTTAAATCTATATTCTTTGGTTGAGTAATAATATTTTCACATCTAATCAAAAGTGATGTGATAGATATTGCATCAAAATGTTGTTATGGATAAGCATGAAGAAGAACACCACAGCCCGAAATGCAAAAAGGAGTTGTCATAAAGCACAATGCGAACCAGCGCTATGCTACAAGCGGAGTCACGTCTTTTCTTTTCAGAGAAATAGGCAGAATTCAATTCATAACTTACCAACGCCAGGTATGTCACGGGCTTCTACTGTTTCCCAGTACTATCTATTATAAATTTGGCATATTTTGGTAGATTTTTTGTTTTAGTTTTTTCTTTAACTATTAATATTCACAAATGCTGTGTGTGGTGATGGTAGGATTTTGTAGTGAGAAATGCCACGGGTTGTGGATCTACCATTGGTCCAATACTTGCTTCTGGAGCTTCCATCCGTACTGTGGATTGTGGTATCCCTCAACTTTCTATGCGCAGGTACAACATACATAGCTGA

mRNA sequence

CCAAATTTTAAAATCTATGTATATAACAATCAATTGGATGTCTGTGAAGAACCGGACGAAGAAATGGCGGGAATATCTCGCTTGCAAGTGCATCTCCTTCACTCTACTCCTCCCGCTCTCAAGTCGACTTCGCTCCTCTCGATGTTTCCTCGCTTTTCTCGCTCTACTCCGCGTAAAATTTTCACTCCTCGTCTTCTCTGTTCGACTCCTCAGAGTTCTTCTTCGGAGGCTGGTTCGAGTTCGAGCAGTGTTGGTGATCTTCTCGATTATCTCAATGAGTCCTGGACTCAGTTTCATTCTACAGCCGAATCGAAACGGCATTTAGTTGCTGCTGGTTTTCATTTGCTAAACGAGGATGAAGAGTGGGACTTAAAAACCTGGTGGATGCTACTTTTTTACACGAAATATGTCTTGTTTGGTTGCCTTTTCCATAGGAGAAAATTTGTAGCTTTCCTTGCGTCAAGCTATTGTGCTTTGAGAGCTCTTATTGATTCTTGTGAATCGCCTAGTGACTTAAAGAGTGAACAGGCAGTTCGAATGGTTGCTTTATTTGATATTGAAGAGGTGGGCTCAGGTTCAATTCAGGAAGCTGGTGCACCCACCATGTTTCAGGCCATGAGGCGCATAGCCAGCTGCTTAGGCCAAGGATACGTTGGTGAAGGTGCTGTTGAGCGCGCTTTTAGGCAATCATTTCTCGCGCTATGCTACAAGCGGAGTCACGTCTTTTCTTTTCAGAGAAATAGGCAGAATTCAATTCATAACTTACCAACGCCAGTGAGAAATGCCACGGGTTGTGGATCTACCATTGGTCCAATACTTGCTTCTGGAGCTTCCATCCGTACTGTGGATTGTGGTATCCCTCAACTTTCTATGCGCAGGTACAACATACATAGCTGA

Coding sequence (CDS)

ATGGCGGGAATATCTCGCTTGCAAGTGCATCTCCTTCACTCTACTCCTCCCGCTCTCAAGTCGACTTCGCTCCTCTCGATGTTTCCTCGCTTTTCTCGCTCTACTCCGCGTAAAATTTTCACTCCTCGTCTTCTCTGTTCGACTCCTCAGAGTTCTTCTTCGGAGGCTGGTTCGAGTTCGAGCAGTGTTGGTGATCTTCTCGATTATCTCAATGAGTCCTGGACTCAGTTTCATTCTACAGCCGAATCGAAACGGCATTTAGTTGCTGCTGGTTTTCATTTGCTAAACGAGGATGAAGAGTGGGACTTAAAAACCTGGTGGATGCTACTTTTTTACACGAAATATGTCTTGTTTGGTTGCCTTTTCCATAGGAGAAAATTTGTAGCTTTCCTTGCGTCAAGCTATTGTGCTTTGAGAGCTCTTATTGATTCTTGTGAATCGCCTAGTGACTTAAAGAGTGAACAGGCAGTTCGAATGGTTGCTTTATTTGATATTGAAGAGGTGGGCTCAGGTTCAATTCAGGAAGCTGGTGCACCCACCATGTTTCAGGCCATGAGGCGCATAGCCAGCTGCTTAGGCCAAGGATACGTTGGTGAAGGTGCTGTTGAGCGCGCTTTTAGGCAATCATTTCTCGCGCTATGCTACAAGCGGAGTCACGTCTTTTCTTTTCAGAGAAATAGGCAGAATTCAATTCATAACTTACCAACGCCAGTGAGAAATGCCACGGGTTGTGGATCTACCATTGGTCCAATACTTGCTTCTGGAGCTTCCATCCGTACTGTGGATTGTGGTATCCCTCAACTTTCTATGCGCAGGTACAACATACATAGCTGA

Protein sequence

MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLCSTPQSSSSEAGSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFYTKYVLFGCLFHRRKFVAFLASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPTMFQAMRRIASCLGQGYVGEGAVERAFRQSFLALCYKRSHVFSFQRNRQNSIHNLPTPVRNATGCGSTIGPILASGASIRTVDCGIPQLSMRRYNIHS
Homology
BLAST of Carg18988 vs. NCBI nr
Match: KAG7012948.1 (putative aspartyl aminopeptidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 550.8 bits (1418), Expect = 6.6e-153
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLCSTPQSSSSEAGSSS 60
           MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLCSTPQSSSSEAGSSS
Sbjct: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLCSTPQSSSSEAGSSS 60

Query: 61  SSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFYTKYVLFGC 120
           SSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFYTKYVLFGC
Sbjct: 61  SSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFYTKYVLFGC 120

Query: 121 LFHRRKFVAFLASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPT 180
           LFHRRKFVAFLASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPT
Sbjct: 121 LFHRRKFVAFLASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPT 180

Query: 181 MFQAMRRIASCLGQGYVGEGAVERAFRQSFLALCYKRSHVFSFQRNRQNSIHNLPTPVRN 240
           MFQAMRRIASCLGQGYVGEGAVERAFRQSFLALCYKRSHVFSFQRNRQNSIHNLPTPVRN
Sbjct: 181 MFQAMRRIASCLGQGYVGEGAVERAFRQSFLALCYKRSHVFSFQRNRQNSIHNLPTPVRN 240

Query: 241 ATGCGSTIGPILASGASIRTVDCGIPQLSMRRYNIHS 278
           ATGCGSTIGPILASGASIRTVDCGIPQLSMRRYNIHS
Sbjct: 241 ATGCGSTIGPILASGASIRTVDCGIPQLSMRRYNIHS 277

BLAST of Carg18988 vs. NCBI nr
Match: KAG6573883.1 (putative aspartyl aminopeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 307.8 bits (787), Expect = 9.8e-80
Identity = 179/249 (71.89%), Postives = 186/249 (74.70%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLCSTPQSSSSEAGSSS 60
           MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLCSTPQSSSSEAGSSS
Sbjct: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLCSTPQSSSSEAGSSS 60

Query: 61  SSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLL-----------NEDEEWDLKTWWML 120
           SSVGDLLDYLNESWTQFHSTAESKRHLVAA   ++              EE      W++
Sbjct: 61  SSVGDLLDYLNESWTQFHSTAESKRHLVAADLQIIPLLCHSIYVAYETTEE-----IWLV 120

Query: 121 LFYTKYVLFGCLFHRRKFVAFLASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEV- 180
              T +  F C +   +FVAFLASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEV 
Sbjct: 121 GHLTCFARF-CSWSTHQFVAFLASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVP 180

Query: 181 --------------------------GSGSIQEAGAPTMFQAMRRIASCLGQGYVGEGAV 212
                                       GSIQEAGAPTMFQAMRRIASCLGQGYVGEGAV
Sbjct: 181 THLAQILYSIPSILSKMSAASRSFRSSEGSIQEAGAPTMFQAMRRIASCLGQGYVGEGAV 240

BLAST of Carg18988 vs. NCBI nr
Match: XP_031742173.1 (probable aspartyl aminopeptidase [Cucumis sativus] >KAE8648830.1 hypothetical protein Csa_009063 [Cucumis sativus])

HSP 1 Score: 268.9 bits (686), Expect = 5.0e-68
Identity = 210/491 (42.77%), Postives = 221/491 (45.01%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQ+ LLH T P+LKS S+ S FP FSRS+PRK F PRLLC    STPQ+SSSEA
Sbjct: 1   MAAISRLQLQLLHFT-PSLKSPSIFSRFPHFSRSSPRKFFPPRLLCSVSDSTPQNSSSEA 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY---- 120
           GSSSS VGDLLDYLNESWTQFH+TAE+KR LVAAGFHLL+EDEEWDLK      F     
Sbjct: 61  GSSSSIVGDLLDYLNESWTQFHATAEAKRQLVAAGFHLLDEDEEWDLKPGGCYFFTRNMS 120

Query: 121 --------TKYV-------------------------------------LFGCLFH---- 180
                    KYV                                       G L+H    
Sbjct: 121 CLVAFSIGEKYVPGNGFHVIAAHTDSPCLKLKPKSSSNKCNCLMVNVQTYGGGLWHTWFD 180

Query: 181 -------------------------RR--------------------------------- 240
                                    RR                                 
Sbjct: 181 RDLSVAGRVIVRGSDGSYLHKLVKVRRPLLRIPTLAIHLDRTVNQDGFKPNLETQLIPLL 240

Query: 241 -----------------------------------------KFVAF-------------- 271
                                                      V+F              
Sbjct: 241 ATKTEDNSVELKDKSNDSFLKDSIHPLLKQVISEELCCAADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. NCBI nr
Match: XP_004150844.3 (LOW QUALITY PROTEIN: probable aspartyl aminopeptidase [Cucumis sativus])

HSP 1 Score: 268.1 bits (684), Expect = 8.6e-68
Identity = 210/491 (42.77%), Postives = 220/491 (44.81%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQ+ LLH T P+LKS S+ S FP FSRS+PRK F PRLLC    STPQ+SSSEA
Sbjct: 1   MAAISRLQLQLLHFT-PSLKSPSIFSRFPHFSRSSPRKFFPPRLLCSVSDSTPQNSSSEA 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY---- 120
           GSSSS VGDLLDYLNESWTQFH+TAE+KR LVAAGFHLL+EDEEWDLK      F     
Sbjct: 61  GSSSSIVGDLLDYLNESWTQFHATAEAKRQLVAAGFHLLDEDEEWDLKPGGCYFFTRNMS 120

Query: 121 --------TKYV-------------------------------------LFGCLFH---- 180
                    KYV                                       G L+H    
Sbjct: 121 CLVAFSIGEKYVPGNGFHVIAAHTDSPCLKLKPKSSSNKCNCLMVNVQTYGGGLWHTWFD 180

Query: 181 -------------------------RR--------------------------------- 240
                                    RR                                 
Sbjct: 181 RDLSVAGRVIVRGSDGSYLHKLVKVRRPLLRIPTLAIHLDRTVNQDGFKPNLETQLIPLL 240

Query: 241 -----------------------------------------KFVAF-------------- 271
                                                      V+F              
Sbjct: 241 ATKTEDNSVELKDKSNDSFLKDSIHPLLKQVISEELCCAADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. NCBI nr
Match: XP_038891318.1 (probable aspartyl aminopeptidase [Benincasa hispida])

HSP 1 Score: 266.5 bits (680), Expect = 2.5e-67
Identity = 205/491 (41.75%), Postives = 218/491 (44.40%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQ+ LLH TPP  KS S+ S FP FSR++PR+ FTPR LC    STPQ+SSSE 
Sbjct: 1   MAAISRLQLQLLHFTPPPFKSPSIFSRFPHFSRTSPRRFFTPRPLCSVSDSTPQNSSSEV 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY---- 120
            SSSS VGDLLDYLNESWTQFH+TAE+KR LVAAGFHLLNEDEEWDLK      F     
Sbjct: 61  ASSSSIVGDLLDYLNESWTQFHATAEAKRQLVAAGFHLLNEDEEWDLKPGGCYFFTRNMS 120

Query: 121 --------TKYV-------------------------------------LFGCLFH---- 180
                    KYV                                       G L+H    
Sbjct: 121 CLVAFSIGEKYVPGNGFHVIAAHTDSPCLKLKPKSSSNKCNCLMVNVQTYGGGLWHTWFD 180

Query: 181 -------------------------RR--------------------------------- 240
                                    RR                                 
Sbjct: 181 RDLSVAGRVIVRGGDGSFLHKLVKVRRPLLRIPTLAIHLDRTVNQDGFKPNLESHLIPLL 240

Query: 241 -----------------------------------------KFVAF-------------- 271
                                                      V+F              
Sbjct: 241 ATKTEDNSLESKDKSNDSFLKDALHPLLKQVISEELCCSADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. ExPASy Swiss-Prot
Match: B9RAJ0 (Probable aspartyl aminopeptidase OS=Ricinus communis OX=3988 GN=RCOM_1506700 PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.9e-22
Identity = 75/178 (42.13%), Postives = 88/178 (49.44%), Query Frame = 0

Query: 131 LASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPTMFQAMRRIAS 190
           L  S+C+L+ALID+  S S L++E  VRMVALFD EEVGS S Q AG+P MF A+ RI S
Sbjct: 280 LCMSFCSLKALIDATASDSHLENESGVRMVALFDHEEVGSDSAQGAGSPVMFDALSRITS 339

Query: 191 CLGQGYVGEGAVERAFRQSFLALC-----------------------------------Y 250
                      + +A ++SFL                                      Y
Sbjct: 340 TFNS---DSKLLRKAIQKSFLVSADMAHALHPNYADKHEENHQPRMHGGLVIKHNANQRY 399

Query: 251 KRSHVFSFQRNRQNSIHNLPTP---VRNATGCGSTIGPILASGASIRTVDCGIPQLSM 271
             + V SF      S HNLP     VRN   CGSTIGPILASG  IRTVD G PQLSM
Sbjct: 400 ATNSVTSFLFKEIASKHNLPVQDFVVRNDMPCGSTIGPILASGVGIRTVDVGAPQLSM 454

BLAST of Carg18988 vs. ExPASy Swiss-Prot
Match: Q9ULA0 (Aspartyl aminopeptidase OS=Homo sapiens OX=9606 GN=DNPEP PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.1e-14
Identity = 60/174 (34.48%), Postives = 81/174 (46.55%), Query Frame = 0

Query: 131 LASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPTMFQAMRRIAS 190
           L S +CAL+ALIDSC  P  L +E  VRMV L+D EEVGS S Q A +      +RRI++
Sbjct: 276 LHSCFCALQALIDSCAGPGSLATEPHVRMVTLYDNEEVGSESAQGAQSLLTELVLRRISA 335

Query: 191 CLGQGYVGEGAVERAFRQSF-LALCYKRSHVFSFQRNRQNSIHNLPT------------- 250
                   E A+ ++F  S  +A     +++   + N +   H  P              
Sbjct: 336 SCQHPTAFEEAIPKSFMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNA 395

Query: 251 --------------------PVRNATGCGSTIGPILASGASIRTVDCGIPQLSM 271
                                VRN T CG+TIGPILAS   +R +D G PQL+M
Sbjct: 396 VSEALIREVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLRVLDLGSPQLAM 449

BLAST of Carg18988 vs. ExPASy Swiss-Prot
Match: Q5RBT2 (Aspartyl aminopeptidase OS=Pongo abelii OX=9601 GN=DNPEP PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.5e-14
Identity = 59/174 (33.91%), Postives = 81/174 (46.55%), Query Frame = 0

Query: 131 LASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPTMFQAMRRIAS 190
           L S +CAL+ALIDSC  P  L +E  VRM+ L+D EEVGS S Q A +      +RRI++
Sbjct: 262 LHSCFCALQALIDSCAGPGSLATEPHVRMITLYDNEEVGSESAQGAQSLLTELVLRRISA 321

Query: 191 CLGQGYVGEGAVERAFRQSF-LALCYKRSHVFSFQRNRQNSIHNLPT------------- 250
                   E A+ ++F  S  +A     +++   + N +   H  P              
Sbjct: 322 SCQHPTAFEEAIPKSFMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNA 381

Query: 251 --------------------PVRNATGCGSTIGPILASGASIRTVDCGIPQLSM 271
                                VRN T CG+TIGPILAS   +R +D G PQL+M
Sbjct: 382 VSEALIREVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLRVLDLGSPQLAM 435

BLAST of Carg18988 vs. ExPASy Swiss-Prot
Match: Q2HJH1 (Aspartyl aminopeptidase OS=Bos taurus OX=9913 GN=DNPEP PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.7e-14
Identity = 57/174 (32.76%), Postives = 84/174 (48.28%), Query Frame = 0

Query: 131 LASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPTMFQAMRRIAS 190
           L S +CAL+ALIDSC +P+ L ++  VRM+AL+D EEVGS S Q A +      +RRI++
Sbjct: 262 LHSCFCALQALIDSCSAPASLAADPHVRMIALYDNEEVGSESAQGAQSLLTELVLRRISA 321

Query: 191 CLGQGYVGEGAVERAFRQSF-LALCYKRSHVFSFQRNRQNSIHNLPT------------- 250
                   E A+ +++  S  +A     +++   + N +   H  P              
Sbjct: 322 SPQHLTAFEEAIPKSYMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNA 381

Query: 251 --------------------PVRNATGCGSTIGPILASGASIRTVDCGIPQLSM 271
                                VRN + CG+TIGPILAS   +R +D G PQL+M
Sbjct: 382 VSEALIREVASSVGVPLQDLMVRNDSPCGTTIGPILASRLGLRVLDLGSPQLAM 435

BLAST of Carg18988 vs. ExPASy Swiss-Prot
Match: Q9Z2W0 (Aspartyl aminopeptidase OS=Mus musculus OX=10090 GN=Dnpep PE=1 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 2.2e-13
Identity = 59/174 (33.91%), Postives = 82/174 (47.13%), Query Frame = 0

Query: 131 LASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPTMFQAMRRIAS 190
           L S +CAL+ALIDSC SP+ L  +  VRMV L+D EEVGS S Q A +      +RRI++
Sbjct: 264 LHSCFCALQALIDSCASPASLARDPHVRMVTLYDNEEVGSESAQGAQSLLTELILRRISA 323

Query: 191 CLGQGYVGEGAVERAFRQSF-LALCYKRSHVFSFQRNRQNSIHNLPT------------- 250
              +    E A+ ++F  S  +A     ++    + N +   H  P              
Sbjct: 324 SPQRLTAFEEAIPKSFMISADMAHAVHPNYSDKHEENHRPLFHKGPVIKVNSKQRYASNA 383

Query: 251 --------------------PVRNATGCGSTIGPILASGASIRTVDCGIPQLSM 271
                                VRN + CG+TIGPILAS   +R +D G PQL+M
Sbjct: 384 VSESMIREVAGQVGVPLQDLMVRNDSPCGTTIGPILASRLGLRVLDLGSPQLAM 437

BLAST of Carg18988 vs. ExPASy TrEMBL
Match: A0A0A0KUS9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G615200 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.9e-68
Identity = 208/491 (42.36%), Postives = 219/491 (44.60%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQ+ LLH T P+LKS S+ S FP FSRS+PRK F PRLLC    STPQ+SSSEA
Sbjct: 1   MAAISRLQLQLLHFT-PSLKSPSIFSRFPHFSRSSPRKFFPPRLLCSVSDSTPQNSSSEA 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFYTKYV 120
           GSSSS VGDLLDYLNESWTQFH+TAE+KR LVAAGFHLL+EDEEWDLK      F     
Sbjct: 61  GSSSSIVGDLLDYLNESWTQFHATAEAKRQLVAAGFHLLDEDEEWDLKPGGCYFFTRNMS 120

Query: 121 LF-------------------------------------------------GCLFH---- 180
            F                                                 G L+H    
Sbjct: 121 CFVAFSIGEKYVPGNGFHVIAAHTDSPCLKLKPKSSSNKCNCLMVNVQTYGGGLWHTWFD 180

Query: 181 -------------------------RR--------------------------------- 240
                                    RR                                 
Sbjct: 181 RDLSVAGRVIVRGSDGSYLHKLVKVRRPLLRIPTLAIHLDRTVNQDGFKPNLETQLIPLL 240

Query: 241 -----------------------------------------KFVAF-------------- 271
                                                      V+F              
Sbjct: 241 ATKTEDNSVELKDKSNDSFLKDSIHPLLKQVISEELCCAADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. ExPASy TrEMBL
Match: A0A6J1DC87 (probable aspartyl aminopeptidase OS=Momordica charantia OX=3673 GN=LOC111018753 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 1.0e-66
Identity = 204/491 (41.55%), Postives = 215/491 (43.79%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQVHLLH TPPALKS++LLS FPR SR++ R+    R LC    STPQSSSSE+
Sbjct: 1   MAAISRLQVHLLHFTPPALKSSALLSRFPRSSRASLRRFVALRPLCSVSDSTPQSSSSES 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY---- 120
           GSSSS VGDL+DYLNESWTQFH+TAE+KR LVAAGF LLNEDEEWDLK      F     
Sbjct: 61  GSSSSIVGDLIDYLNESWTQFHATAEAKRQLVAAGFRLLNEDEEWDLKPGGCYFFTRNMS 120

Query: 121 --------TKYV-----------------------------------------LFGCLFH 180
                    KYV                                         L+   F 
Sbjct: 121 CLVAFSIGEKYVAGNGFHIIAAHTDSPCLKLKPRSSSSKCNCLMVNVQTYGSGLWHTWFD 180

Query: 181 RRKFVA------------------------------------------------------ 240
           R   VA                                                      
Sbjct: 181 RDLSVAGRVIVRGNDGSYLHKLVKVRRPLLRIPTLAIHLDRKVNQDGFKPNLETHLIPLL 240

Query: 241 ------------------------------------------------------------ 271
                                                                       
Sbjct: 241 ATKLEDSSLDSKDKSTDAFLKDALHPLLKQVLSEELCCAADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. ExPASy TrEMBL
Match: A0A5A7SZY8 (Putative aspartyl aminopeptidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006780 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.7e-66
Identity = 206/491 (41.96%), Postives = 218/491 (44.40%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQ+ LLH T P+LKS S+ S FP FSRS+PRK   PRLLC    STPQ+SSSE 
Sbjct: 1   MAAISRLQLQLLHFT-PSLKSPSIFSRFPHFSRSSPRKFLPPRLLCSVSDSTPQNSSSEV 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY---- 120
           GSSSS VGDLLDYLNESWTQFH+TAE+KR LVAAGFHLLNEDEEWDLK      F     
Sbjct: 61  GSSSSIVGDLLDYLNESWTQFHATAEAKRQLVAAGFHLLNEDEEWDLKPGGCYFFTRNMS 120

Query: 121 --------TKYV-------------------------------------LFGCLFH---- 180
                    KYV                                       G L+H    
Sbjct: 121 CLVAFSIGEKYVPGNGFHVIAAHTDSPCLKLKPKSSSKKCNCLMVNVQTYGGGLWHTWFD 180

Query: 181 -------------------------RR--------------------------------- 240
                                    RR                                 
Sbjct: 181 RDLSVAGRVIVRGSDGSYLHKLVKVRRPLLRIPTLAIHLDRTVNQDGFKPNLETQLIPLL 240

Query: 241 -----------------------------------------KFVAF-------------- 271
                                                      V+F              
Sbjct: 241 ATKTEDNSLELKDKSNDSFLKDAIHPLLKQVISEELCCAADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. ExPASy TrEMBL
Match: A0A5D3CD05 (Putative aspartyl aminopeptidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002030 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.7e-66
Identity = 206/491 (41.96%), Postives = 218/491 (44.40%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQ+ LLH T P+LKS S+ S FP FSRS+PRK   PRLLC    STPQ+SSSE 
Sbjct: 1   MAAISRLQLQLLHFT-PSLKSPSIFSRFPHFSRSSPRKFLPPRLLCSVSDSTPQNSSSEV 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY---- 120
           GSSSS VGDLLDYLNESWTQFH+TAE+KR LVAAGFHLLNEDEEWDLK      F     
Sbjct: 61  GSSSSIVGDLLDYLNESWTQFHATAEAKRQLVAAGFHLLNEDEEWDLKPGGCYFFTRNMS 120

Query: 121 --------TKYV-------------------------------------LFGCLFH---- 180
                    KYV                                       G L+H    
Sbjct: 121 CLVAFSIGEKYVPGNGFHVIAAHTDSPCLKLKPKSSSNKCNCLMVNVQTYGGGLWHTWFD 180

Query: 181 -------------------------RR--------------------------------- 240
                                    RR                                 
Sbjct: 181 RDLSVAGRVIVRGSDGSYLHKLVKVRRPLLRIPTLAIHLDRTVNQDGFKPNLETQLIPLL 240

Query: 241 -----------------------------------------KFVAF-------------- 271
                                                      V+F              
Sbjct: 241 ATKTEDNSLELKDKSNDSFLKDAIHPLLKQVISEELCCAADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. ExPASy TrEMBL
Match: A0A1S3BGN8 (probable aspartyl aminopeptidase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489395 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.7e-66
Identity = 206/491 (41.96%), Postives = 218/491 (44.40%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSMFPRFSRSTPRKIFTPRLLC----STPQSSSSEA 60
           MA ISRLQ+ LLH T P+LKS S+ S FP FSRS+PRK   PRLLC    STPQ+SSSE 
Sbjct: 1   MAAISRLQLQLLHFT-PSLKSPSIFSRFPHFSRSSPRKFLPPRLLCSVSDSTPQNSSSEV 60

Query: 61  GSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY---- 120
           GSSSS VGDLLDYLNESWTQFH+TAE+KR LVAAGFHLLNEDEEWDLK      F     
Sbjct: 61  GSSSSIVGDLLDYLNESWTQFHATAEAKRQLVAAGFHLLNEDEEWDLKPGGCYFFTRNMS 120

Query: 121 --------TKYV-------------------------------------LFGCLFH---- 180
                    KYV                                       G L+H    
Sbjct: 121 CLVAFSIGEKYVPGNGFHVIAAHTDSPCLKLKPKSSSNKCNCLMVNVQTYGGGLWHTWFD 180

Query: 181 -------------------------RR--------------------------------- 240
                                    RR                                 
Sbjct: 181 RDLSVAGRVIVRGSDGSYLHKLVKVRRPLLRIPTLAIHLDRTVNQDGFKPNLETQLIPLL 240

Query: 241 -----------------------------------------KFVAF-------------- 271
                                                      V+F              
Sbjct: 241 ATKTEDNSLELKDKSNDSFLKDAIHPLLKQVISEELCCAADDIVSFELNVCDTQPSCLGG 300

BLAST of Carg18988 vs. TAIR 10
Match: AT5G04710.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 3.4e-38
Identity = 155/491 (31.57%), Postives = 182/491 (37.07%), Query Frame = 0

Query: 1   MAGISRLQVHLLHSTPPALKSTSLLSM---FPRFSRSTPRKIF---TPRLLCSTPQSSSS 60
           MA I+RL   L HS P     +S LS    FP +   +P + F   +P L  S   S S 
Sbjct: 1   MAAIARLP--LTHSLPSIFNPSSFLSQSLSFPTYLHRSPFRSFSSVSPILCTSHRDSRSP 60

Query: 61  EAGSSSSSVGDLLDYLNESWTQFHSTAESKRHLVAAGFHLLNEDEEWDLKTWWMLLFY-- 120
            + S++S VGDLLDYLNESWTQFH+TAE+KR L+AAGF LL+E+E+W+LK      F   
Sbjct: 61  GSDSNASIVGDLLDYLNESWTQFHATAEAKRQLLAAGFDLLSENEDWNLKPGGRYFFTRN 120

Query: 121 ----------TKYV-----------------------------------------LFGCL 180
                      KYV                                         L+   
Sbjct: 121 MSCLVAFAVGEKYVPGNGFHAIAAHTDSPCLKLKPKSASSKSGYLMVNVQTYGGGLWHTW 180

Query: 181 FHRRKFVA---------------------------------------------------- 240
           F R   VA                                                    
Sbjct: 181 FDRDLSVAGRAIVRASDGSFVHRLVKVKRPLLRVPTLAIHLDRTVNSDGFKPNLETQLVP 240

Query: 241 ------------------------------------------------------------ 271
                                                                       
Sbjct: 241 LLATKSDESSAESKDKNVSSKDAHHPLLMQILSDDLDCKVEDIVSLELNICDTQPSCLGG 300

BLAST of Carg18988 vs. TAIR 10
Match: AT5G60160.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-23
Identity = 73/178 (41.01%), Postives = 86/178 (48.31%), Query Frame = 0

Query: 131 LASSYCALRALIDSCESPSDLKSEQAVRMVALFDIEEVGSGSIQEAGAPTMFQAMRRIAS 190
           L  S+C+L+ALID+  S SDL+ E  +RMVALFD EEVGS S Q AG+P M  AM  I S
Sbjct: 266 LCMSFCSLKALIDATSSGSDLEDESGIRMVALFDHEEVGSNSAQGAGSPVMIDAMSHITS 325

Query: 191 CLGQGYVGEGAVERAFRQSFLALC-----------------------------------Y 250
           C          +++A ++S L                                      Y
Sbjct: 326 CFSS---DTKVLKKAIQKSLLVSADMAHALHPNFMDKHEENHQPKMHGGLVIKHNANQRY 385

Query: 251 KRSHVFSFQRNRQNSIHNLPTP---VRNATGCGSTIGPILASGASIRTVDCGIPQLSM 271
             + V SF        HNLP     VRN  GCGSTIGPILAS   IRTVD G PQLSM
Sbjct: 386 ATNAVTSFVFREIAEKHNLPVQDFVVRNDMGCGSTIGPILASSVGIRTVDVGAPQLSM 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012948.16.6e-153100.00putative aspartyl aminopeptidase [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6573883.19.8e-8071.89putative aspartyl aminopeptidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_031742173.15.0e-6842.77probable aspartyl aminopeptidase [Cucumis sativus] >KAE8648830.1 hypothetical pr... [more]
XP_004150844.38.6e-6842.77LOW QUALITY PROTEIN: probable aspartyl aminopeptidase [Cucumis sativus][more]
XP_038891318.12.5e-6741.75probable aspartyl aminopeptidase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
B9RAJ01.9e-2242.13Probable aspartyl aminopeptidase OS=Ricinus communis OX=3988 GN=RCOM_1506700 PE=... [more]
Q9ULA01.1e-1434.48Aspartyl aminopeptidase OS=Homo sapiens OX=9606 GN=DNPEP PE=1 SV=2[more]
Q5RBT21.5e-1433.91Aspartyl aminopeptidase OS=Pongo abelii OX=9601 GN=DNPEP PE=2 SV=1[more]
Q2HJH19.7e-1432.76Aspartyl aminopeptidase OS=Bos taurus OX=9913 GN=DNPEP PE=1 SV=1[more]
Q9Z2W02.2e-1333.91Aspartyl aminopeptidase OS=Mus musculus OX=10090 GN=Dnpep PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KUS91.9e-6842.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G615200 PE=3 SV=1[more]
A0A6J1DC871.0e-6641.55probable aspartyl aminopeptidase OS=Momordica charantia OX=3673 GN=LOC111018753 ... [more]
A0A5A7SZY81.7e-6641.96Putative aspartyl aminopeptidase isoform X1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A5D3CD051.7e-6641.96Putative aspartyl aminopeptidase isoform X1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BGN81.7e-6641.96probable aspartyl aminopeptidase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489... [more]
Match NameE-valueIdentityDescription
AT5G04710.13.4e-3831.57Zn-dependent exopeptidases superfamily protein [more]
AT5G60160.11.4e-2341.01Zn-dependent exopeptidases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001948Peptidase M18PFAMPF02127Peptidase_M18coord: 132..194
e-value: 7.9E-8
score: 31.5
coord: 236..270
e-value: 9.3E-9
score: 34.6
IPR001948Peptidase M18PANTHERPTHR28570ASPARTYL AMINOPEPTIDASEcoord: 129..211
IPR001948Peptidase M18PANTHERPTHR28570ASPARTYL AMINOPEPTIDASEcoord: 38..111
IPR001948Peptidase M18PANTHERPTHR28570ASPARTYL AMINOPEPTIDASEcoord: 215..271
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 131..215
e-value: 8.6E-17
score: 63.0
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 218..273
e-value: 5.1E-11
score: 44.0
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 59..118
e-value: 6.1E-7
score: 30.7
NoneNo IPR availablePANTHERPTHR28570:SF14ASPARTYL AMINOPEPTIDASEcoord: 38..111
NoneNo IPR availablePANTHERPTHR28570:SF14ASPARTYL AMINOPEPTIDASEcoord: 129..211
coord: 215..271
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 62..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18988-RACarg18988-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004177 aminopeptidase activity
molecular_function GO:0008270 zinc ion binding