Carg18894 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18894
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontranscription factor bHLH147-like
LocationCarg_Chr08: 7741711 .. 7742936 (+)
RNA-Seq ExpressionCarg18894
SyntenyCarg18894
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAATGCAAAGAGTAAGTAAAGAAAGGCGGAAGGAGTCCCAAGGCTTTGGGTGTATGGCTAGGGCTGAATAAAATAGGAGTGAGATCCGAATCAATAAATATGAATTCTGGTTGCTTCACAACTTTATCTCCCTTCTCCCTCTCTCTCTCTACCCACTTGCAGAAGAGGACACCTTAAGAGCGGGTTCCATTTATGTCTTTTCGCTCTCCTAACCCTCTGTTTCATCTTCTTCACAGTTCTTATGTTTTGGTTGATCCAGGTTTCGTCTTCGAAGGAATTTCCCCAAAAGGTACGAATCTTATCAACTCCTTCTCTTGCCTTCTTCCTCCTATTATCATTACAATTTTTAATCTTCCTTTCTCCGGTTCCCAAGAATTTTCAATTTCTATTCTTCATTTGGGAGTTTCCGTTTCTGTTAGTCTAAATCTGTCATTTCCTTTTGATCCTTCCTTCCTCAGGATATCTTCTTTGCTTGCTCTTGTTCCGTTAGGTGAAACATAAATTTGGAGAAGAGAGATTGGATCATTGGTCCATCTTTGAATCGTCGATTTGTTGAGGGTAACCAATTTTTGTTTTTGTTTTTTTTTTTTTTGAGATCGGATCGGTGCGTCTTGATGGCGTCATCTGTTCTTTCGAACTCCGATCGCATGCGGGAGTCTTCAAGGAAGCAAAAGAAGAAGAAAAACCACGGCAACAGAGACCGTCATCAAAACCAGAACCATATTAACTGGAAATCTCAAGCTCAGAAAGAAATTTACTCGTCTAAGCTGGTTCGAGCGTTGAATCAAGTGAGGCTCAGTCCTTCGAATGAGGCGCCCCGCCGTGGTAGAGCCGTACGCGAGGCCGCCGACAGAATCCTTGCCGTCGCCGCCAAGGGGAGAACCAGGTGGAGCCGAGCCATTCTAACGAATAGGTTGAAGCTCAAGTTCAGGAAGCCGAAGAAACAGAGAGCATCCACCGCTGGAAACAACCGCCCAAAGAAACCTAGAGTCAGCGTATTGCGGTTGAAAGGAAAAACGTTGCCGGCGGTGCAAAGGAAAGTTAGGGTTCTTGGACGGCTAGTTCCTGGTTGCCGGAAAGAACCTTGGCCGGTTATCCTAGAAGAGGCCACCGATTACATAGCTGCACTTGAAATGCAAGTTCGAGCTATGACTGCCCTCGCCGAGTTGCTCTCGGCCTCAACTTCTGTTGCAGGCTCTAGCTCCGCTCCTCCAAGCTGA

mRNA sequence

ATGGCTGAATGCAAAGAGTTTCGTCTTCGAAGGAATTTCCCCAAAAGATCGGATCGGTGCGTCTTGATGGCGTCATCTGTTCTTTCGAACTCCGATCGCATGCGGGAGTCTTCAAGGAAGCAAAAGAAGAAGAAAAACCACGGCAACAGAGACCGTCATCAAAACCAGAACCATATTAACTGGAAATCTCAAGCTCAGAAAGAAATTTACTCGTCTAAGCTGGTTCGAGCGTTGAATCAAGTGAGGCTCAGTCCTTCGAATGAGGCGCCCCGCCGTGGTAGAGCCGTACGCGAGGCCGCCGACAGAATCCTTGCCGTCGCCGCCAAGGGGAGAACCAGGTGGAGCCGAGCCATTCTAACGAATAGGTTGAAGCTCAAGTTCAGGAAGCCGAAGAAACAGAGAGCATCCACCGCTGGAAACAACCGCCCAAAGAAACCTAGAGTCAGCGTATTGCGGTTGAAAGGAAAAACGTTGCCGGCGGTGCAAAGGAAAGTTAGGGTTCTTGGACGGCTAGTTCCTGGTTGCCGGAAAGAACCTTGGCCGGTTATCCTAGAAGAGGCCACCGATTACATAGCTGCACTTGAAATGCAAGTTCGAGCTATGACTGCCCTCGCCGAGTTGCTCTCGGCCTCAACTTCTGTTGCAGGCTCTAGCTCCGCTCCTCCAAGCTGA

Coding sequence (CDS)

ATGGCTGAATGCAAAGAGTTTCGTCTTCGAAGGAATTTCCCCAAAAGATCGGATCGGTGCGTCTTGATGGCGTCATCTGTTCTTTCGAACTCCGATCGCATGCGGGAGTCTTCAAGGAAGCAAAAGAAGAAGAAAAACCACGGCAACAGAGACCGTCATCAAAACCAGAACCATATTAACTGGAAATCTCAAGCTCAGAAAGAAATTTACTCGTCTAAGCTGGTTCGAGCGTTGAATCAAGTGAGGCTCAGTCCTTCGAATGAGGCGCCCCGCCGTGGTAGAGCCGTACGCGAGGCCGCCGACAGAATCCTTGCCGTCGCCGCCAAGGGGAGAACCAGGTGGAGCCGAGCCATTCTAACGAATAGGTTGAAGCTCAAGTTCAGGAAGCCGAAGAAACAGAGAGCATCCACCGCTGGAAACAACCGCCCAAAGAAACCTAGAGTCAGCGTATTGCGGTTGAAAGGAAAAACGTTGCCGGCGGTGCAAAGGAAAGTTAGGGTTCTTGGACGGCTAGTTCCTGGTTGCCGGAAAGAACCTTGGCCGGTTATCCTAGAAGAGGCCACCGATTACATAGCTGCACTTGAAATGCAAGTTCGAGCTATGACTGCCCTCGCCGAGTTGCTCTCGGCCTCAACTTCTGTTGCAGGCTCTAGCTCCGCTCCTCCAAGCTGA

Protein sequence

MAECKEFRLRRNFPKRSDRCVLMASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVRLSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMTALAELLSASTSVAGSSSAPPS
Homology
BLAST of Carg18894 vs. NCBI nr
Match: KAG7026360.1 (Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 410.6 bits (1054), Expect = 8.6e-111
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 1   MAECKEFRLRRNFPKRSDRCVLMASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHIN 60
           MAECKEFRLRRNFPKRSDRCVLMASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHIN
Sbjct: 1   MAECKEFRLRRNFPKRSDRCVLMASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHIN 60

Query: 61  WKSQAQKEIYSSKLVRALNQVRLSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILT 120
           WKSQAQKEIYSSKLVRALNQVRLSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILT
Sbjct: 61  WKSQAQKEIYSSKLVRALNQVRLSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILT 120

Query: 121 NRLKLKFRKPKKQRASTAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPW 180
           NRLKLKFRKPKKQRASTAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPW
Sbjct: 121 NRLKLKFRKPKKQRASTAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPW 180

Query: 181 PVILEEATDYIAALEMQVRAMTALAELLSASTSVAGSSSAPPS 224
           PVILEEATDYIAALEMQVRAMTALAELLSASTSVAGSSSAPPS
Sbjct: 181 PVILEEATDYIAALEMQVRAMTALAELLSASTSVAGSSSAPPS 223

BLAST of Carg18894 vs. NCBI nr
Match: KAG6594020.1 (Transcription factor basic helix-loop-helix 147, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365.2 bits (936), Expect = 4.1e-97
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR
Sbjct: 1   MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 142
           LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR
Sbjct: 61  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 120

Query: 143 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT 202
           PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT
Sbjct: 121 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT 180

Query: 203 ALAELLSASTSVAGSSSAPPS 224
           ALAELLSASTSVAGSSSAPPS
Sbjct: 181 ALAELLSASTSVAGSSSAPPS 201

BLAST of Carg18894 vs. NCBI nr
Match: XP_023514807.1 (transcription factor bHLH147-like [Cucurbita pepo subsp. pepo] >XP_023514808.1 transcription factor bHLH147-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 358.6 bits (919), Expect = 3.9e-95
Identity = 199/201 (99.00%), Postives = 200/201 (99.50%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR
Sbjct: 1   MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 142
           LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR
Sbjct: 61  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 120

Query: 143 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT 202
           PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEP PVILEEATDYIAALEMQVRAM+
Sbjct: 121 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPLPVILEEATDYIAALEMQVRAMS 180

Query: 203 ALAELLSASTSVAGSSSAPPS 224
           ALAELLSASTSVAGSSSAPPS
Sbjct: 181 ALAELLSASTSVAGSSSAPPS 201

BLAST of Carg18894 vs. NCBI nr
Match: XP_022930482.1 (transcription factor bHLH147-like [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 1.5e-94
Identity = 199/201 (99.00%), Postives = 199/201 (99.00%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR
Sbjct: 1   MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 142
           LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAG NR
Sbjct: 61  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGINR 120

Query: 143 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT 202
           PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEP PVILEEATDYIAALEMQVRAMT
Sbjct: 121 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPLPVILEEATDYIAALEMQVRAMT 180

Query: 203 ALAELLSASTSVAGSSSAPPS 224
           ALAELLSASTSVAGSSSAPPS
Sbjct: 181 ALAELLSASTSVAGSSSAPPS 201

BLAST of Carg18894 vs. NCBI nr
Match: XP_022999891.1 (transcription factor bHLH147-like [Cucurbita maxima])

HSP 1 Score: 347.8 bits (891), Expect = 6.8e-92
Identity = 194/200 (97.00%), Postives = 195/200 (97.50%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQN INWKSQAQKEIYSSKLVRALNQVR
Sbjct: 1   MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNLINWKSQAQKEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 142
           LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQR STAGNNR
Sbjct: 61  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRVSTAGNNR 120

Query: 143 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT 202
           PKKPRVSV RLKGKTLPAVQRKVRVLGRLVP CRKEP PVILEEATDYIAALEMQVRAM+
Sbjct: 121 PKKPRVSVFRLKGKTLPAVQRKVRVLGRLVPSCRKEPLPVILEEATDYIAALEMQVRAMS 180

Query: 203 ALAELLSASTSVAGSSSAPP 223
           ALAELLSASTSVAGSSSAPP
Sbjct: 181 ALAELLSASTSVAGSSSAPP 200

BLAST of Carg18894 vs. ExPASy Swiss-Prot
Match: Q9LSN7 (Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 6.7e-42
Identity = 110/206 (53.40%), Postives = 144/206 (69.90%), Query Frame = 0

Query: 21  VLMASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHI--NWKSQAQKEIYSSKLVRAL 80
           +L  ++  SNSDR R   RK+KKK +  + ++  + +     W+S+ Q++IYS+KLV AL
Sbjct: 11  LLQPTTTSSNSDRSR---RKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHAL 70

Query: 81  NQVRL------SPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKK 140
            ++R+      S S+  PR GRAVRE ADR LAVAA+G+T WSRAIL+  +KLKFRK K+
Sbjct: 71  RELRISQQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKR 130

Query: 141 QRAS-------TAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILE 200
           QR S       T G+ R KK R +VLRLK K LPAVQRKV+VL RLVPGCRK+  PV+LE
Sbjct: 131 QRISNPTTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLE 190

Query: 201 EATDYIAALEMQVRAMTALAELLSAS 212
           E TDYIAA+EMQ+R MTA+   +S+S
Sbjct: 191 ETTDYIAAMEMQIRTMTAILSAVSSS 213

BLAST of Carg18894 vs. ExPASy Swiss-Prot
Match: Q9C8Z9 (Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-39
Identity = 111/214 (51.87%), Postives = 139/214 (64.95%), Query Frame = 0

Query: 25  SSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVRL- 84
           +S  S+  R ++ S       +  +      + H  W+S+ Q+ IYS+KL +AL QVRL 
Sbjct: 12  TSTTSDLVRRKKRSSASSAASSRSSASSVSGEIHARWRSEKQQRIYSAKLFQALQQVRLN 71

Query: 85  -----SPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTA 144
                S S  A +RG+AVREAADR LAV+A+GRT WSRAIL NR+KLKFRK ++ RA+ A
Sbjct: 72  SSASTSSSPTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMA 131

Query: 145 ----------GNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEAT 204
                      +NR +K RVSVLRL  K++P V RKVRVLGRLVPGC K+  PVILEEAT
Sbjct: 132 IPAMTTVVSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEAT 191

Query: 205 DYIAALEMQVRAMTALAELLSASTSVAGSSSAPP 223
           DYI ALEMQVRAM +L +LLS+        SAPP
Sbjct: 192 DYIQALEMQVRAMNSLVQLLSS------YGSAPP 219

BLAST of Carg18894 vs. ExPASy Swiss-Prot
Match: O80482 (Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-28
Identity = 91/202 (45.05%), Postives = 130/202 (64.36%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGN----RDRHQNQNHI-NWKSQAQKEIYSSKLVRA 82
           M  S+  + +   ESSR++K + +         R  N+  +  WK+   ++IY+ KLV A
Sbjct: 1   MVESLFPSIENTGESSRRKKPRISETAEAEIEARRVNEESLKRWKTNRVQQIYACKLVEA 60

Query: 83  LNQVR----LSPSNEAPR----RGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRK 142
           L +VR     + +NE  +      R +R+ ADR+LA +A+G TRWSRAIL +R++ K +K
Sbjct: 61  LRRVRQRSSTTSNNETDKLVSGAAREIRDTADRVLAASARGTTRWSRAILASRVRAKLKK 120

Query: 143 PKKQRASTAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATD 202
            +K + ST GN + +K      R+K   LPAV+RK+++LGRLVPGCRK   P +L+EATD
Sbjct: 121 HRKAKKST-GNCKSRKGLTETNRIK---LPAVERKLKILGRLVPGCRKVSVPNLLDEATD 180

Query: 203 YIAALEMQVRAMTALAELLSAS 212
           YIAALEMQVRAM ALAELL+A+
Sbjct: 181 YIAALEMQVRAMEALAELLTAA 198

BLAST of Carg18894 vs. ExPASy Swiss-Prot
Match: Q9M9L6 (Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.6e-21
Identity = 66/154 (42.86%), Postives = 90/154 (58.44%), Query Frame = 0

Query: 61  WKSQAQKEIYSSKLVRALNQVRLSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILT 120
           W++   +++YS KL  AL         +  RR   VR+ AD++LA  A+G TRWSRAIL 
Sbjct: 63  WRATTAQKVYSLKLYDAL---------QRSRRSATVRDTADKVLATTARGATRWSRAILV 122

Query: 121 NRLKLKFRKPKKQRASTAGNNRPKKPRVSVLRLKG-----KTLPAVQRKVRVLGRLVPGC 180
           +R     R+ +        N +P     + +R  G     + L AV  +VRVLG LVPGC
Sbjct: 123 SRFGTSLRRRR--------NTKPASALAAAIRGSGGSGRRRKLSAVGNRVRVLGGLVPGC 182

Query: 181 RKEPWPVILEEATDYIAALEMQVRAMTALAELLS 210
           R+   P +L+E  DYIAALEMQVRAMTAL+++LS
Sbjct: 183 RRTALPELLDETADYIAALEMQVRAMTALSKILS 199

BLAST of Carg18894 vs. ExPASy TrEMBL
Match: A0A6J1EQM7 (transcription factor bHLH147-like OS=Cucurbita moschata OX=3662 GN=LOC111436924 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 7.1e-95
Identity = 199/201 (99.00%), Postives = 199/201 (99.00%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR
Sbjct: 1   MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 142
           LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAG NR
Sbjct: 61  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGINR 120

Query: 143 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT 202
           PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEP PVILEEATDYIAALEMQVRAMT
Sbjct: 121 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPLPVILEEATDYIAALEMQVRAMT 180

Query: 203 ALAELLSASTSVAGSSSAPPS 224
           ALAELLSASTSVAGSSSAPPS
Sbjct: 181 ALAELLSASTSVAGSSSAPPS 201

BLAST of Carg18894 vs. ExPASy TrEMBL
Match: A0A6J1KED1 (transcription factor bHLH147-like OS=Cucurbita maxima OX=3661 GN=LOC111494233 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.3e-92
Identity = 194/200 (97.00%), Postives = 195/200 (97.50%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQN INWKSQAQKEIYSSKLVRALNQVR
Sbjct: 1   MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNLINWKSQAQKEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTAGNNR 142
           LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQR STAGNNR
Sbjct: 61  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRVSTAGNNR 120

Query: 143 PKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAMT 202
           PKKPRVSV RLKGKTLPAVQRKVRVLGRLVP CRKEP PVILEEATDYIAALEMQVRAM+
Sbjct: 121 PKKPRVSVFRLKGKTLPAVQRKVRVLGRLVPSCRKEPLPVILEEATDYIAALEMQVRAMS 180

Query: 203 ALAELLSASTSVAGSSSAPP 223
           ALAELLSASTSVAGSSSAPP
Sbjct: 181 ALAELLSASTSVAGSSSAPP 200

BLAST of Carg18894 vs. ExPASy TrEMBL
Match: A0A6J1H465 (transcription factor bHLH147-like OS=Cucurbita moschata OX=3662 GN=LOC111460328 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.7e-86
Identity = 184/202 (91.09%), Postives = 193/202 (95.54%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSV SNSDR RESSR+QKKKK+HGNRD HQNQNHI WKSQAQ+EIYSSKLVRALNQVR
Sbjct: 1   MASSVHSNSDRARESSRRQKKKKSHGNRDHHQNQNHIKWKSQAQQEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQR-ASTAGNN 142
           LSP NEAPRRGRAVREAADR+LAVAAKGRTRWSRAILTNRLKLKFRKPK+QR ASTAGNN
Sbjct: 61  LSPPNEAPRRGRAVREAADRVLAVAAKGRTRWSRAILTNRLKLKFRKPKRQRSASTAGNN 120

Query: 143 RPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAM 202
           R KKPRVSVLRL+GK+LPAVQRKVRVLGRLVPGCRKEP PVILEEATDYIAALEMQVRAM
Sbjct: 121 RSKKPRVSVLRLRGKSLPAVQRKVRVLGRLVPGCRKEPLPVILEEATDYIAALEMQVRAM 180

Query: 203 TALAELLSASTSVAGSSSAPPS 224
           +ALAELLSASTS AGSSSAPP+
Sbjct: 181 SALAELLSASTSAAGSSSAPPN 202

BLAST of Carg18894 vs. ExPASy TrEMBL
Match: A0A6J1L1P5 (transcription factor bHLH147-like OS=Cucurbita maxima OX=3661 GN=LOC111499053 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.9e-85
Identity = 182/202 (90.10%), Postives = 191/202 (94.55%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVR 82
           MASSV SNSDR RESSR+QKKKK+HGNRD HQNQNHI WKSQAQ+EIYSSKLVRALNQVR
Sbjct: 1   MASSVHSNSDRARESSRRQKKKKSHGNRDHHQNQNHIKWKSQAQQEIYSSKLVRALNQVR 60

Query: 83  LSPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQR-ASTAGNN 142
           LSP NE PRRGRAVREAADR+LAVAAKGRTRWSRAIL NRLKLKFRKPK+QR ASTAGNN
Sbjct: 61  LSPPNEGPRRGRAVREAADRVLAVAAKGRTRWSRAILKNRLKLKFRKPKRQRSASTAGNN 120

Query: 143 RPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQVRAM 202
           R KKPRVSVLRL+GK+LPAVQRKVRVLGRLVPGCRKEP PVILEEATDYIAALEMQVRAM
Sbjct: 121 RSKKPRVSVLRLRGKSLPAVQRKVRVLGRLVPGCRKEPLPVILEEATDYIAALEMQVRAM 180

Query: 203 TALAELLSASTSVAGSSSAPPS 224
           +ALAELLSASTS AGSSSAPP+
Sbjct: 181 SALAELLSASTSAAGSSSAPPN 202

BLAST of Carg18894 vs. ExPASy TrEMBL
Match: A0A0A0KCL4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G327940 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.2e-81
Identity = 185/206 (89.81%), Postives = 194/206 (94.17%), Query Frame = 0

Query: 23  MASSVLSNSDRMRE-SSRKQKKKKNHGNRDRHQNQN-HINWKSQAQKEIYSSKLVRALNQ 82
           MASSVLSNSDR RE SSRKQKKKK+HGNRD HQNQN HI WKSQAQ+EIYSSKLVRALNQ
Sbjct: 1   MASSVLSNSDRARESSSRKQKKKKSHGNRDHHQNQNHHIKWKSQAQQEIYSSKLVRALNQ 60

Query: 83  VRLSPSNEA-PRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRK-PKKQRA-ST 142
           VRL PSNEA PRRGRAVREAADR+LAVAAKGRTRWSRAILTNRLKLKFRK PK+QR+ ST
Sbjct: 61  VRLGPSNEAPPRRGRAVREAADRVLAVAAKGRTRWSRAILTNRLKLKFRKAPKRQRSTST 120

Query: 143 AGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATDYIAALEMQ 202
           AGNNR KKPRVSVLRL+GK+LPAVQRKVRVLGRLVPGCRKEP PVILEEATDYIAALEMQ
Sbjct: 121 AGNNRSKKPRVSVLRLRGKSLPAVQRKVRVLGRLVPGCRKEPLPVILEEATDYIAALEMQ 180

Query: 203 VRAMTALAELLSASTSVAGSSSAPPS 224
           VRAM+ALAELLSASTS AGSSS+PPS
Sbjct: 181 VRAMSALAELLSASTSAAGSSSSPPS 206

BLAST of Carg18894 vs. TAIR 10
Match: AT3G17100.1 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 172.2 bits (435), Expect = 4.8e-43
Identity = 110/206 (53.40%), Postives = 144/206 (69.90%), Query Frame = 0

Query: 21  VLMASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHI--NWKSQAQKEIYSSKLVRAL 80
           +L  ++  SNSDR R   RK+KKK +  + ++  + +     W+S+ Q++IYS+KLV AL
Sbjct: 11  LLQPTTTSSNSDRSR---RKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHAL 70

Query: 81  NQVRL------SPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKK 140
            ++R+      S S+  PR GRAVRE ADR LAVAA+G+T WSRAIL+  +KLKFRK K+
Sbjct: 71  RELRISQQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKR 130

Query: 141 QRAS-------TAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILE 200
           QR S       T G+ R KK R +VLRLK K LPAVQRKV+VL RLVPGCRK+  PV+LE
Sbjct: 131 QRISNPTTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLE 190

Query: 201 EATDYIAALEMQVRAMTALAELLSAS 212
           E TDYIAA+EMQ+R MTA+   +S+S
Sbjct: 191 ETTDYIAAMEMQIRTMTAILSAVSSS 213

BLAST of Carg18894 vs. TAIR 10
Match: AT3G17100.2 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 172.2 bits (435), Expect = 4.8e-43
Identity = 110/206 (53.40%), Postives = 144/206 (69.90%), Query Frame = 0

Query: 21  VLMASSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHI--NWKSQAQKEIYSSKLVRAL 80
           +L  ++  SNSDR R   RK+KKK +  + ++  + +     W+S+ Q++IYS+KLV AL
Sbjct: 11  LLQPTTTSSNSDRSR---RKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHAL 70

Query: 81  NQVRL------SPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKK 140
            ++R+      S S+  PR GRAVRE ADR LAVAA+G+T WSRAIL+  +KLKFRK K+
Sbjct: 71  RELRISQQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKR 130

Query: 141 QRAS-------TAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILE 200
           QR S       T G+ R KK R +VLRLK K LPAVQRKV+VL RLVPGCRK+  PV+LE
Sbjct: 131 QRISNPTTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLE 190

Query: 201 EATDYIAALEMQVRAMTALAELLSAS 212
           E TDYIAA+EMQ+R MTA+   +S+S
Sbjct: 191 ETTDYIAAMEMQIRTMTAILSAVSSS 213

BLAST of Carg18894 vs. TAIR 10
Match: AT3G06590.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.0e-40
Identity = 111/214 (51.87%), Postives = 139/214 (64.95%), Query Frame = 0

Query: 25  SSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVRL- 84
           +S  S+  R ++ S       +  +      + H  W+S+ Q+ IYS+KL +AL QVRL 
Sbjct: 12  TSTTSDLVRRKKRSSASSAASSRSSASSVSGEIHARWRSEKQQRIYSAKLFQALQQVRLN 71

Query: 85  -----SPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTA 144
                S S  A +RG+AVREAADR LAV+A+GRT WSRAIL NR+KLKFRK ++ RA+ A
Sbjct: 72  SSASTSSSPTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMA 131

Query: 145 ----------GNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEAT 204
                      +NR +K RVSVLRL  K++P V RKVRVLGRLVPGC K+  PVILEEAT
Sbjct: 132 IPAMTTVVSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEAT 191

Query: 205 DYIAALEMQVRAMTALAELLSASTSVAGSSSAPP 223
           DYI ALEMQVRAM +L +LLS+        SAPP
Sbjct: 192 DYIQALEMQVRAMNSLVQLLSS------YGSAPP 219

BLAST of Carg18894 vs. TAIR 10
Match: AT3G06590.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.0e-40
Identity = 111/214 (51.87%), Postives = 139/214 (64.95%), Query Frame = 0

Query: 25  SSVLSNSDRMRESSRKQKKKKNHGNRDRHQNQNHINWKSQAQKEIYSSKLVRALNQVRL- 84
           +S  S+  R ++ S       +  +      + H  W+S+ Q+ IYS+KL +AL QVRL 
Sbjct: 12  TSTTSDLVRRKKRSSASSAASSRSSASSVSGEIHARWRSEKQQRIYSAKLFQALQQVRLN 71

Query: 85  -----SPSNEAPRRGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRKPKKQRASTA 144
                S S  A +RG+AVREAADR LAV+A+GRT WSRAIL NR+KLKFRK ++ RA+ A
Sbjct: 72  SSASTSSSPTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMA 131

Query: 145 ----------GNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEAT 204
                      +NR +K RVSVLRL  K++P V RKVRVLGRLVPGC K+  PVILEEAT
Sbjct: 132 IPAMTTVVSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEAT 191

Query: 205 DYIAALEMQVRAMTALAELLSASTSVAGSSSAPP 223
           DYI ALEMQVRAM +L +LLS+        SAPP
Sbjct: 192 DYIQALEMQVRAMNSLVQLLSS------YGSAPP 219

BLAST of Carg18894 vs. TAIR 10
Match: AT1G09250.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-29
Identity = 91/202 (45.05%), Postives = 130/202 (64.36%), Query Frame = 0

Query: 23  MASSVLSNSDRMRESSRKQKKKKNHGN----RDRHQNQNHI-NWKSQAQKEIYSSKLVRA 82
           M  S+  + +   ESSR++K + +         R  N+  +  WK+   ++IY+ KLV A
Sbjct: 1   MVESLFPSIENTGESSRRKKPRISETAEAEIEARRVNEESLKRWKTNRVQQIYACKLVEA 60

Query: 83  LNQVR----LSPSNEAPR----RGRAVREAADRILAVAAKGRTRWSRAILTNRLKLKFRK 142
           L +VR     + +NE  +      R +R+ ADR+LA +A+G TRWSRAIL +R++ K +K
Sbjct: 61  LRRVRQRSSTTSNNETDKLVSGAAREIRDTADRVLAASARGTTRWSRAILASRVRAKLKK 120

Query: 143 PKKQRASTAGNNRPKKPRVSVLRLKGKTLPAVQRKVRVLGRLVPGCRKEPWPVILEEATD 202
            +K + ST GN + +K      R+K   LPAV+RK+++LGRLVPGCRK   P +L+EATD
Sbjct: 121 HRKAKKST-GNCKSRKGLTETNRIK---LPAVERKLKILGRLVPGCRKVSVPNLLDEATD 180

Query: 203 YIAALEMQVRAMTALAELLSAS 212
           YIAALEMQVRAM ALAELL+A+
Sbjct: 181 YIAALEMQVRAMEALAELLTAA 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7026360.18.6e-111100.00Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6594020.14.1e-97100.00Transcription factor basic helix-loop-helix 147, partial [Cucurbita argyrosperma... [more]
XP_023514807.13.9e-9599.00transcription factor bHLH147-like [Cucurbita pepo subsp. pepo] >XP_023514808.1 t... [more]
XP_022930482.11.5e-9499.00transcription factor bHLH147-like [Cucurbita moschata][more]
XP_022999891.16.8e-9297.00transcription factor bHLH147-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LSN76.7e-4253.40Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=... [more]
Q9C8Z91.4e-3951.87Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=... [more]
O804821.5e-2845.05Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=... [more]
Q9M9L66.6e-2142.86Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1EQM77.1e-9599.00transcription factor bHLH147-like OS=Cucurbita moschata OX=3662 GN=LOC111436924 ... [more]
A0A6J1KED13.3e-9297.00transcription factor bHLH147-like OS=Cucurbita maxima OX=3661 GN=LOC111494233 PE... [more]
A0A6J1H4652.7e-8691.09transcription factor bHLH147-like OS=Cucurbita moschata OX=3662 GN=LOC111460328 ... [more]
A0A6J1L1P53.9e-8590.10transcription factor bHLH147-like OS=Cucurbita maxima OX=3661 GN=LOC111499053 PE... [more]
A0A0A0KCL41.2e-8189.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G327940 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G17100.14.8e-4353.40sequence-specific DNA binding transcription factors [more]
AT3G17100.24.8e-4353.40sequence-specific DNA binding transcription factors [more]
AT3G06590.11.0e-4051.87basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G06590.21.0e-4051.87basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G09250.11.0e-2945.05basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..64
NoneNo IPR availablePANTHERPTHR33124:SF78TRANSCRIPTION FACTOR BHLH148-LIKE ISOFORM X1coord: 36..222
IPR044660Transcription factor IBH1-likePANTHERPTHR33124TRANSCRIPTION FACTOR IBH1-LIKE 1coord: 36..222
IPR044549Transcription factor IBH1-like, bHLH domainCDDcd11444bHLH_AtIBH1_likecoord: 150..205
e-value: 5.56243E-16
score: 67.6476
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 164..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18894-RACarg18894-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000976 transcription cis-regulatory region binding