Carg18889 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18889
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	3-hydroxybutyryl-CoA dehydratase-like protein
Location	Carg_Chr08: 7724388 .. 7725626 (+)
RNA-Seq Expression	Carg18889
Synteny	Carg18889

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCGTTCTTCGCGGGAAAATCTCATCCTCGTAAACCGAGAACCAAATGCGATCGCGTACCTTACTATCAACCGTCCGAGGTCTCTGAATTCGTTAACGAGGCCGATGATGAGGGATTTGGCTCAGGCGTTTAAGAGCCTGGACAACGACGAGTCCGTTCGGGTCATCGTGCTCTCTGGATCAGGACGAGCGTTTTACTGCCTTGGAGGACGTGTTTAAGGGAGATGTGAAGGATGTTGAATACAATCCGGTCGTGCATATGGAACGTTGCCGGAAGCCGATCATCGGAGCGATTGATTGCAGGCTTCGCCATCACCGCCGCGTTTGAGATCGCGTTGGCCTAAGTGACATACGCATCGCTGCAAAAGGAGCTAAGTTCGTCGATACTCATGCTAGGTTTGTCGTTTTACTTAGTTCACTCTTGTTTTGTGTGCTCGAGCGATTCTTGCTGGTTATAGTAGCTAGGATTAGGGCAAAGACGATTTGATTTTCTGAAATCCACTGGCAGTACTGTTCTATTGAAATTACCAATTTCTGGAAATGTTTGTGCAGATTTGGGATATTCCCTTCATGAGGGTTATCTCAGAAACTTCCACGAACAATCGGAGTAAACAAAGGTCGTGAAGCATCCCTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGTAAAGGGGTTTGGTAAATCAGGTGGTTGAAGGAGGTGATTTGTTGAAGAAATCTAGAGAGGTTGCAGAGGCCATTTTAAAGAACAAGTCGGCCATTAACGATGGCCTGAAGCTGGATCTTGGCCAAGCTCTTGCTCTGGAACAGGTAATTGCCTTCCCGAAGCTGTTGATTATTGAACCTTGAAATTCTCATTCTGACATCTTCTAAAACTCGAAGTTTATCTCCAAATTGGTACCTAACATTTGAGTGTGGATAAGCTTTCCATCTTGAAAGGTCAATGGTTCAATCATGAACTCAGCATAAATTTGAAGCACCTTTTGCCACTGGCTGATTGTTTTGTTGATGCAGGAGAGAGCTCATGAGTATTACAATGGAATGACCAAAGAGCAATTTCAGAAAATGCAGGACTTCATAGCTGGTCGGGCCTCCAAGAACATGTAGTTTCCTCAACGGTCAGTAGATTCCTCCCTACTTCCATTTGCATCTGTAATTTAATTCTAGTGAGTCAGAAGCCTCTGTCCCATTTGTTTCAAATTGCAGTCCAGGTAGTCAATTTTTTTTTTTTTTTT

mRNA sequence

ATGGATCGTTCTTCGCGGGAAAATCTCATCCTCGTAAACCGAGAACCAAATGCGATCGCGTACCTTACTATCAACCGTCCGAGGTCTCTGAATTCGTTAACGAGGCCGATGATGAGGGATTTGGCTCAGGCGTTTAAGAGCCTGGACAACGACGAGTCCGTTCGGGTCATCGTGCTCTCTGGATCAGGACGAGCGTTTTACTGCCTTGGAGGACGTGGAGATGTGAAGGATGTTGAATACAATCCGGTCGTGCATATGGAACGTTGCCGGAAGCCGATCATCGGAGCGATTGATTGCAGGCTTCGCCATCACCGCCGCATTTGGGATATTCCCTTCATGAGGAAACTTCCACGAACAATCGGAGTAAACAAAGGTCGTGAAGCATCCCTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGGGTTTGGTAAATCAGGTGGTTGAAGGAGGTGATTTGTTGAAGAAATCTAGAGAGGTTGCAGAGGCCATTTTAAAGAACAAGTCGGCCATTAACGATGGCCTGAAGCTGGATCTTGGCCAAGCTCTTGCTCTGGAACAGGAGAGAGCTCATGAGTATTACAATGGAATGACCAAAGAGCAATTTCAGAAAATGCAGGACTTCATAGCTGGTCGGGCCTCCAAGAACATGTAGTTTCCTCAACGGTCAGTAGATTCCTCCCTACTTCCATTTGCATCTGTAATTTAATTCTAGTGAGTCAGAAGCCTCTGTCCCATTTGTTTCAAATTGCAGTCCAGGTAGTCAATTTTTTTTTTTTTTTT

Coding sequence (CDS)

ATGGATCGTTCTTCGCGGGAAAATCTCATCCTCGTAAACCGAGAACCAAATGCGATCGCGTACCTTACTATCAACCGTCCGAGGTCTCTGAATTCGTTAACGAGGCCGATGATGAGGGATTTGGCTCAGGCGTTTAAGAGCCTGGACAACGACGAGTCCGTTCGGGTCATCGTGCTCTCTGGATCAGGACGAGCGTTTTACTGCCTTGGAGGACGTGGAGATGTGAAGGATGTTGAATACAATCCGGTCGTGCATATGGAACGTTGCCGGAAGCCGATCATCGGAGCGATTGATTGCAGGCTTCGCCATCACCGCCGCATTTGGGATATTCCCTTCATGAGGAAACTTCCACGAACAATCGGAGTAAACAAAGGTCGTGAAGCATCCCTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGGGTTTGGTAAATCAGGTGGTTGAAGGAGGTGATTTGTTGAAGAAATCTAGAGAGGTTGCAGAGGCCATTTTAAAGAACAAGTCGGCCATTAACGATGGCCTGAAGCTGGATCTTGGCCAAGCTCTTGCTCTGGAACAGGAGAGAGCTCATGAGTATTACAATGGAATGACCAAAGAGCAATTTCAGAAAATGCAGGACTTCATAGCTGGTCGGGCCTCCAAGAACATGTAG

Protein sequence

MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCLGGRGDVKDVEYNPVVHMERCRKPIIGAIDCRLRHHRRIWDIPFMRKLPRTIGVNKGREASLTAMPLTAEEAEGLVNQVVEGGDLLKKSREVAEAILKNKSAINDGLKLDLGQALALEQERAHEYYNGMTKEQFQKMQDFIAGRASKNM

Homology

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BLAST of Carg18889 vs. NCBI nr
Match: XP_023006283.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima])

HSP 1 Score: 286.6 bits (732), Expect = 1.8e-73
Identity = 169/260 (65.00%), Postives = 186/260 (71.54%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFKSLD DESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           G+GRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GTGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ERAH YYN
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYN 240

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BLAST of Carg18889 vs. NCBI nr
Match: XP_023006284.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima])

HSP 1 Score: 286.2 bits (731), Expect = 2.4e-73
Identity = 169/260 (65.00%), Postives = 186/260 (71.54%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFKSLD DESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           G+GRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GTGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ERAH YYN
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYN 240

[Close]

BLAST of Carg18889 vs. NCBI nr
Match: XP_022959404.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 286.2 bits (731), Expect = 2.4e-73
Identity = 169/260 (65.00%), Postives = 186/260 (71.54%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFK+LD DESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           GSGRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GSGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ERAH YYN
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYN 240

[Close]

BLAST of Carg18889 vs. NCBI nr
Match: KAG6575117.1 (putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.9 bits (725), Expect = 1.2e-72
Identity = 168/260 (64.62%), Postives = 185/260 (71.15%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFK+LD DESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           GSGRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GSGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ERAH YY 
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYT 240

[Close]

BLAST of Carg18889 vs. NCBI nr
Match: XP_023548460.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 283.9 bits (725), Expect = 1.2e-72
Identity = 168/260 (64.62%), Postives = 185/260 (71.15%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFKSLD DESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           GSGRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GSGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGV+K RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ER H YYN
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYN 240

[Close]

BLAST of Carg18889 vs. ExPASy Swiss-Prot
Match: Q6NL24 (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECHIA PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 6.3e-61
Identity = 142/260 (54.62%), Postives = 170/260 (65.38%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MD++  ENLI V +E   IA +TINRP+SLNSLTR MM DLA+AFK +D+DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAID------------- 120
           GSGR+F C G          +GDVKD E +PVV MER RKPIIGAI+             
Sbjct: 61  GSGRSF-CSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KL R IG NK RE SLT+MPLTA+ A   G 
Sbjct: 121 CDILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGF 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VVE G+ LKK+RE+AEAI+KN        KS INDGLKLDLG AL LE+ERAH YY+
Sbjct: 181 VNHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYS 240

[Close]

BLAST of Carg18889 vs. ExPASy Swiss-Prot
Match: P52046 (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=crt PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.2e-15
Identity = 67/255 (26.27%), Postives = 119/255 (46.67%), Query Frame = 0


Query: 8   NLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFY 67
           N +++ +E   +A +TINRP++LN+L    ++++      ++ND  V  ++L+G+G   +
Sbjct: 4   NNVILEKE-GKVAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSF 63

Query: 68  CLGGRGDVKDV-EYNPVV-------------HMERCRKPIIGAI---------------D 127
             G   D+ ++ E N +               +E   KP+I A+               D
Sbjct: 64  VAG--ADISEMKEMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCD 123

Query: 128 CRLRHHRRIWDIPFM-----------RKLPRTIGVNKGREASLTAMPLTAEEA--EGLVN 187
            R+      +  P +           ++L R +G+   ++   TA  + A+EA   GLVN
Sbjct: 124 IRIASSNARFGQPEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVN 183

Query: 188 QVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYNGM 213
           +VVE  +L+  ++E+A  I+ N        K AIN G++ D+  ALA E E   E ++  
Sbjct: 184 KVVEPSELMNTAKEIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS-- 243

[Close]

BLAST of Carg18889 vs. ExPASy Swiss-Prot
Match: Q1ZXF1 (Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=echs1 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.1e-12
Identity = 66/242 (27.27%), Postives = 112/242 (46.28%), Query Frame = 0


Query: 19  IAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCLGGRGDVKDV 78
           +  +T+NRP+SLN+L+  ++ ++  A K    D+ V  I+++GS +AF       D+K++
Sbjct: 34  VGLVTLNRPKSLNALSDGLISEINSAVKLFQEDKDVGSIIITGSEKAF---AAGADIKEM 93

Query: 79  E-------YNPVV-----HMERCRKPIIGAI---------------DCRLRHHRRIWDIP 138
           E       YN  +      + + RKPII A+               D  +   + ++  P
Sbjct: 94  EKVTLPDAYNNDLLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGEKAVFGQP 153

Query: 139 FMR-----------KLPRTIGVNKGREASLTAMPLTAEEAE--GLVNQVVEGGDLLKKSR 198
            ++           +L R IG +K  E  LT   LTA EAE  GLV++VV   +LL ++ 
Sbjct: 154 EIKLGTIPGCGGTQRLIRAIGKSKAMELVLTGNNLTAVEAEKAGLVSKVVPVEELLTEAT 213

Query: 199 EVAEAILK--------NKSAINDGLKLDLGQALALEQERAHEYYNGMTKEQFQKMQDFIA 213
           ++AE I           K A+N   +L L + +  E+   H  +   T +Q + M  F+ 
Sbjct: 214 KMAEKIASYSQLTVAMAKEAVNASYELTLQEGIRFERRMFHSTFG--THDQKEGMNAFVE 270

[Close]

BLAST of Carg18889 vs. ExPASy Swiss-Prot
Match: P64017 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA8 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.1e-11
Identity = 66/249 (26.51%), Postives = 109/249 (43.78%), Query Frame = 0


Query: 10  ILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCL 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD+D  +  I+++GS +AF   
Sbjct: 6   ILVERD-QRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  GGRGDVKDVEYNPVV---------HMERCRKPIIGAI-------DCRL------------ 129
               ++ D+ +              +   R P I A+        C L            
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 ----RHHRRIWDIPFM---RKLPRTIGVNKGREASLTAMPLTAEEAE--GLVNQVVEGGD 189
               +   ++  +P M   ++L R IG  K  +  LT   + A EAE  GLV++VV   D
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKSREVAEAILK--------NKSAINDGLKLDLGQALALEQERAHEYYNGMTKEQFQK 214
           LL ++R  A  I +         K A+N   +  L + L  E+   H  +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

[Close]

BLAST of Carg18889 vs. ExPASy Swiss-Prot
Match: P9WNN8 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=echA8 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.1e-11
Identity = 66/249 (26.51%), Postives = 109/249 (43.78%), Query Frame = 0


Query: 10  ILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCL 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD+D  +  I+++GS +AF   
Sbjct: 6   ILVERD-QRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  GGRGDVKDVEYNPVV---------HMERCRKPIIGAI-------DCRL------------ 129
               ++ D+ +              +   R P I A+        C L            
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 ----RHHRRIWDIPFM---RKLPRTIGVNKGREASLTAMPLTAEEAE--GLVNQVVEGGD 189
               +   ++  +P M   ++L R IG  K  +  LT   + A EAE  GLV++VV   D
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKSREVAEAILK--------NKSAINDGLKLDLGQALALEQERAHEYYNGMTKEQFQK 214
           LL ++R  A  I +         K A+N   +  L + L  E+   H  +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

[Close]

BLAST of Carg18889 vs. ExPASy TrEMBL
Match: A0A6J1L4H0 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.8e-74
Identity = 169/260 (65.00%), Postives = 186/260 (71.54%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFKSLD DESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           G+GRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GTGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ERAH YYN
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYN 240

[Close]

BLAST of Carg18889 vs. ExPASy TrEMBL
Match: A0A6J1L1R6 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.2e-73
Identity = 169/260 (65.00%), Postives = 186/260 (71.54%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFKSLD DESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           G+GRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GTGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ERAH YYN
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYN 240

[Close]

BLAST of Carg18889 vs. ExPASy TrEMBL
Match: A0A6J1H7Z0 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111460389 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.2e-73
Identity = 169/260 (65.00%), Postives = 186/260 (71.54%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS E LILVNREPN IA++TINRP+SLNSLTR MM DLAQAFK+LD DESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           GSGRAF C G          +GDVKDVE +PVV MERCRKPIIGA+              
Sbjct: 61  GSGRAF-CSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KLPR IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VV+GG+LLKK+RE+AEAILKN        KS INDGLKLDLG ALALE+ERAH YYN
Sbjct: 181 VNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYN 240

[Close]

BLAST of Carg18889 vs. ExPASy TrEMBL
Match: A0A1S3C8X4 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497788 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 7.5e-73
Identity = 170/260 (65.38%), Postives = 185/260 (71.15%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS ENLILVNRE N IA++TINRP+SLNSLTRPMM DLA+AFKSLD D+SVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           GSGRAF C G          +GDVKDVE +PV  ME CRKPIIGAI              
Sbjct: 61  GSGRAF-CSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KL R IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VNQVVEG +LLKK+REVAEAILKN        KS INDGLKLDLGQALALE+ERAH YYN
Sbjct: 181 VNQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYN 240

[Close]

BLAST of Carg18889 vs. ExPASy TrEMBL
Match: A0A0A0KCM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330970 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.1e-71
Identity = 167/260 (64.23%), Postives = 182/260 (70.00%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MDRSS ENLILVNRE N IA++TINRP+SLNSLT+ MM DLA AFKSLD D+SVRVI+LS
Sbjct: 1   MDRSSSENLILVNRESNGIAFITINRPKSLNSLTKHMMADLAHAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAI-------------D 120
           GSGRAF C G          +GDVKDVE +PV  ME CRKPIIGAI              
Sbjct: 61  GSGRAF-CSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KL R IGVNK RE SLTAMPLTAEEAE  GL
Sbjct: 121 CDILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGL 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VNQVVEG +LLKK+REVAEAILKN        KS INDGLKLDLGQAL LE+ERAH YYN
Sbjct: 181 VNQVVEGSELLKKAREVAEAILKNNQDLVVRYKSVINDGLKLDLGQALTLEKERAHAYYN 240

[Close]

BLAST of Carg18889 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 235.3 bits (599), Expect = 4.5e-62
Identity = 142/260 (54.62%), Postives = 170/260 (65.38%), Query Frame = 0


Query: 1   MDRSSRENLILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLS 60
           MD++  ENLI V +E   IA +TINRP+SLNSLTR MM DLA+AFK +D+DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFYCLG---------GRGDVKDVEYNPVVHMERCRKPIIGAID------------- 120
           GSGR+F C G          +GDVKD E +PVV MER RKPIIGAI+             
Sbjct: 61  GSGRSF-CSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALA 120

Query: 121 CR----------LRHHRRIWDIP---FMRKLPRTIGVNKGREASLTAMPLTAEEAE--GL 180
           C           +  H R    P     +KL R IG NK RE SLT+MPLTA+ A   G 
Sbjct: 121 CDILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGF 180

Query: 181 VNQVVEGGDLLKKSREVAEAILKN--------KSAINDGLKLDLGQALALEQERAHEYYN 216
           VN VVE G+ LKK+RE+AEAI+KN        KS INDGLKLDLG AL LE+ERAH YY+
Sbjct: 181 VNHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYS 240

[Close]

BLAST of Carg18889 vs. TAIR 10
Match: AT2G30660.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.7e-08
Identity = 30/69 (43.48%), Postives = 44/69 (63.77%), Query Frame = 0


Query: 15 EPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCLGGRGD 74
          E +++  LT+NRP+ LN+L   M+  L Q F++ + D SV++++L G GRAF C G  GD
Sbjct: 11 EKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAF-CAG--GD 70

Query: 75 VKDVEYNPV 84
          V  V  N V
Sbjct: 71 VPPVVQNMV 76

[Close]

BLAST of Carg18889 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 57.4 bits (137), Expect = 1.7e-08
Identity = 50/191 (26.18%), Postives = 83/191 (43.46%), Query Frame = 0


Query: 10  ILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCL 69
           I+     +++ +L INRP  LN+L+     +  +A  SLD +  V VI+LSG+G+ F C 
Sbjct: 11  IIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGAGKHF-CS 70

Query: 70  G-------------------GRGD------VKDVEYNPVVHMERCRKPIIGAID------ 129
           G                   GR        +K ++   +  +E+CRKP+I AI       
Sbjct: 71  GIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQ-AAITAIEQCRKPVIAAIHGACIGG 130

Query: 130 -------CRLRHHRR-------------IWDIPFMRKLPRTIGVNKGREASLTAMPLTAE 148
                  C +R+                + D+  +++LP  +G     E +LTA   +  
Sbjct: 131 GVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTARRFSGS 190

[Close]

BLAST of Carg18889 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 57.4 bits (137), Expect = 1.7e-08
Identity = 50/191 (26.18%), Postives = 83/191 (43.46%), Query Frame = 0


Query: 10  ILVNREPNAIAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCL 69
           I+     +++ +L INRP  LN+L+     +  +A  SLD +  V VI+LSG+G+ F C 
Sbjct: 11  IIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGAGKHF-CS 70

Query: 70  G-------------------GRGD------VKDVEYNPVVHMERCRKPIIGAID------ 129
           G                   GR        +K ++   +  +E+CRKP+I AI       
Sbjct: 71  GIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQ-AAITAIEQCRKPVIAAIHGACIGG 130

Query: 130 -------CRLRHHRR-------------IWDIPFMRKLPRTIGVNKGREASLTAMPLTAE 148
                  C +R+                + D+  +++LP  +G     E +LTA   +  
Sbjct: 131 GVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTARRFSGS 190

[Close]

BLAST of Carg18889 vs. TAIR 10
Match: AT1G60550.1 (enoyl-CoA hydratase/isomerase D )

HSP 1 Score: 56.6 bits (135), Expect = 2.9e-08
Identity = 55/190 (28.95%), Postives = 78/190 (41.05%), Query Frame = 0


Query: 19  IAYLTINRPRSLNSLTRPMMRDLAQAFKSLDNDESVRVIVLSGSGRAFYCLG-------- 78
           IA +TINRP   N+     +++L +AF    +D SV VI+L+G G   +C G        
Sbjct: 87  IAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQALRTQ 146

Query: 79  -GRGDVKDVEYNPV----VHMERCRKPIIGAI---------------DCRLRHHRRI--- 138
            G  D  DV    V    V + R  KP+I  +               D  +     I   
Sbjct: 147 DGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAADNAIFGQ 206

Query: 139 -------WDIPFMRK-LPRTIGVNKGREASLTAMPLTAEEAE--GLVNQVVEGGDLLKKS 168
                  +D  +    + R +G  K RE        TA EAE  GL+N VV   DL K++
Sbjct: 207 TGPKVGSFDAGYGSSIMSRLVGPKKAREMWFMTRFYTASEAEKMGLINTVVPLEDLEKET 266

The following BLAST results are available for this feature:

BLAST of Carg18889 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023006283.1	1.8e-73	65.00	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima]	[more]
XP_023006284.1	2.4e-73	65.00	probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima]	[more]
XP_022959404.1	2.4e-73	65.00	probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata]	[more]
KAG6575117.1	1.2e-72	64.62	putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma sub...	[more]
XP_023548460.1	1.2e-72	64.62	probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg18889 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NL24	6.3e-61	54.62	Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
P52046	5.2e-15	26.27	Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /...	[more]
Q1ZXF1	7.1e-12	27.27	Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689...	[more]
P64017	2.1e-11	26.51	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /...	[more]
P9WNN8	2.1e-11	26.51	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 155...	[more]

BLAST of Carg18889 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L4H0	8.8e-74	65.00	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=36...	[more]
A0A6J1L1R6	1.2e-73	65.00	probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=36...	[more]
A0A6J1H7Z0	1.2e-73	65.00	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A1S3C8X4	7.5e-73	65.38	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497...	[more]
A0A0A0KCM0	3.1e-71	64.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330970 PE=4 SV=1	[more]

BLAST of Carg18889 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16210.1	4.5e-62	54.62	enoyl-CoA hydratase/isomerase A	[more]
AT2G30660.1	1.7e-08	43.48	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT5G43280.1	1.7e-08	26.18	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT5G43280.2	1.7e-08	26.18	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT1G60550.1	2.9e-08	28.95	enoyl-CoA hydratase/isomerase D	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.226.10		coord: 106..215 e-value: 3.7E-12 score: 48.0
None	No IPR available	GENE3D	3.90.226.10		coord: 10..99 e-value: 3.0E-18 score: 68.1
None	No IPR available	PANTHER	PTHR43802	ENOYL-COA HYDRATASE	coord: 114..215 coord: 4..98
None	No IPR available	CDD	cd06558	crotonase-like	coord: 10..164 e-value: 2.50079E-34 score: 119.202
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 15..71 e-value: 6.5E-13 score: 48.6 coord: 114..204 e-value: 2.0E-7 score: 30.6
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 8..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18889-RA	Carg18889-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity