Carg18817 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18817
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	thionin-like protein 2
Location	Carg_Chr08: 3751050 .. 3751695 (+)
RNA-Seq Expression	Carg18817
Synteny	Carg18817

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCCATTGTTGTAATATGCTTCATTCTGAGCTTAGTTGCAGGCAGGTCCACAGCCTCCTTTGGGAAGTGCTACGCCAAGTGCTTCATTGTGTGTGCTATCACACCAGGCGTCCCTATTGGCACATGCGGCGGAAAGTGCTTGGCTGATTGCCTCTTCCCTGCCTCTGCCCCTACAGATCTCAACCATTTGGACACTCATTATTTCTGCAAACTTGGCTGTGCCACTTCTCTCTGCACCAAATTTAGCACCAAGACAGACCCGGGTGGGTTACAAAATTTTTAATACTCTCCTAATATTTGAAAATTTTTTATTGTTAGTATCATTTTTAAATAGCGATTAACGTGTTTATAGCTAACTTTCCTACACGCTTTTGACAATGTTTTTAATGAATTTGGCAGCTGAGAAGAAAGTGGAAAGTTGTGTGAACTCGTGCTCTCGAACATGTTTGAAGGCTTAAAACTGAGAAGGAGCTGGGATTGGCTTTAAAGACAATAAGTGTCGGTGGTGAAAACCAAACACCATTGGACTCTAAGCTTGTCCCATATCTACTAAGCATGTTTTGATATATCTATATGCTTTCTAGTTTCTGCATGTACTCGTCCTCATCACTGTCTAATAATCAAGGTTGCTTTTCCATGCT

mRNA sequence

ATGAAGTCCATTGTTGTAATATGCTTCATTCTGAGCTTAGTTGCAGGCAGGTCCACAGCCTCCTTTGGGAAGTGCTACGCCAAGTGCTTCATTGTGTGTGCTATCACACCAGGCGTCCCTATTGGCACATGCGGCGGAAAGTGCTTGGCTGATTGCCTCTTCCCTGCCTCTGCCCCTACAGATCTCAACCATTTGGACACTCATTATTTCTGCAAACTTGGCTGTGCCACTTCTCTCTGCACCAAATTTAGCACCAAGACAGACCCGGCTGAGAAGAAAGTGGAAAGTTGTGTGAACTCGTGCTCTCGAACATGTTTGAAGGCTTAAAACTGAGAAGGAGCTGGGATTGGCTTTAAAGACAATAAGTGTCGGTGGTGAAAACCAAACACCATTGGACTCTAAGCTTGTCCCATATCTACTAAGCATGTTTTGATATATCTATATGCTTTCTAGTTTCTGCATGTACTCGTCCTCATCACTGTCTAATAATCAAGGTTGCTTTTCCATGCT

Coding sequence (CDS)

ATGAAGTCCATTGTTGTAATATGCTTCATTCTGAGCTTAGTTGCAGGCAGGTCCACAGCCTCCTTTGGGAAGTGCTACGCCAAGTGCTTCATTGTGTGTGCTATCACACCAGGCGTCCCTATTGGCACATGCGGCGGAAAGTGCTTGGCTGATTGCCTCTTCCCTGCCTCTGCCCCTACAGATCTCAACCATTTGGACACTCATTATTTCTGCAAACTTGGCTGTGCCACTTCTCTCTGCACCAAATTTAGCACCAAGACAGACCCGGCTGAGAAGAAAGTGGAAAGTTGTGTGAACTCGTGCTCTCGAACATGTTTGAAGGCTTAA

Protein sequence

MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPTDLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA

Homology

[Close]

BLAST of Carg18817 vs. NCBI nr
Match: KAG7025781.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 227.3 bits (578), Expect = 6.5e-56
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60
           MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT
Sbjct: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60

Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA
Sbjct: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 108

[Close]

BLAST of Carg18817 vs. NCBI nr
Match: KAG6593441.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 224.9 bits (572), Expect = 3.2e-55
Identity = 107/108 (99.07%), Postives = 107/108 (99.07%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60
           MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAP 
Sbjct: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPR 60

Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA
Sbjct: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 108

[Close]

BLAST of Carg18817 vs. NCBI nr
Match: XP_023000407.1 (thionin-like protein 2 [Cucurbita maxima])

HSP 1 Score: 220.7 bits (561), Expect = 6.1e-54
Identity = 105/108 (97.22%), Postives = 106/108 (98.15%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60
           MKS+VVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLF ASAP 
Sbjct: 36  MKSVVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFLASAPR 95

Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA
Sbjct: 96  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 143

[Close]

BLAST of Carg18817 vs. NCBI nr
Match: XP_038899787.1 (thionin-like protein 2 [Benincasa hispida])

HSP 1 Score: 203.4 bits (516), Expect = 1.0e-48
Identity = 91/108 (84.26%), Postives = 100/108 (92.59%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60
           MKS+++ICFI+SLV GRSTASFGKCYAKCF+VCAITPG+PIGTCG KCL DCLF AS+P 
Sbjct: 1   MKSVILICFIMSLVVGRSTASFGKCYAKCFVVCAITPGIPIGTCGAKCLGDCLFIASSPL 60

Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           D NHLDTHYFCKLGCATSLCTKFSTK DPAEKKVESCVNSC +TC+KA
Sbjct: 61  DFNHLDTHYFCKLGCATSLCTKFSTKKDPAEKKVESCVNSCGQTCIKA 108

[Close]

BLAST of Carg18817 vs. NCBI nr
Match: KAG7014098.1 (Thionin-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 186.4 bits (472), Expect = 1.3e-43
Identity = 84/108 (77.78%), Postives = 96/108 (88.89%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60
           MKS+V+ICFILSL+AGRSTASF KCYAKCFI CAITPG+ +GTCG  CLA CLF ASAP 
Sbjct: 1   MKSVVIICFILSLMAGRSTASFRKCYAKCFIACAITPGITLGTCGATCLAKCLFIASAPM 60

Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           D NH++THYFCKLGCATS+CTKFSTK DP+EKKVE CV+SC+ TC+KA
Sbjct: 61  DFNHMNTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 108

[Close]

BLAST of Carg18817 vs. ExPASy Swiss-Prot
Match: A8MRP4 (Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-08
Identity = 37/110 (33.64%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 3   SIVVICFILSLVAGRSTAS--FGKCYAKCFIVCAITPGVP-IGTCGGKCLADCLFPASAP 62
           +++++  I +L+A  +     F +CY  C + C      P    C   C  +CL   S P
Sbjct: 4   AVMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPP 63

Query: 63  T-DLNHLD-THYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLK 108
           +   N++D + YFCKLGCAT  C   S+  +P  ++V +CV+SCS  C K
Sbjct: 64  SVSSNNIDESDYFCKLGCATYHCVSLSSIQNPNVERVSACVDSCSNKCTK 113

[Close]

BLAST of Carg18817 vs. ExPASy TrEMBL
Match: A0A6J1KDK0 (thionin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111494667 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.0e-54
Identity = 105/108 (97.22%), Postives = 106/108 (98.15%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60
           MKS+VVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLF ASAP 
Sbjct: 36  MKSVVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFLASAPR 95

Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA
Sbjct: 96  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 143

[Close]

BLAST of Carg18817 vs. ExPASy TrEMBL
Match: A0A0A0KC18 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446640 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 7.1e-40
Identity = 80/102 (78.43%), Postives = 90/102 (88.24%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT 60
           MKS+V+ICFILSLVAGRSTASFGKCYAKCFIVCAITPG+P+GTCG KCLADCLF AS+P 
Sbjct: 1   MKSVVLICFILSLVAGRSTASFGKCYAKCFIVCAITPGIPVGTCGAKCLADCLFIASSPM 60

Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCS 103
           DLN++DTHYFCKLGCATS CTKFSTK DP +K   + V + S
Sbjct: 61  DLNYMDTHYFCKLGCATSRCTKFSTKKDPGKKLSNTSVTTYS 102

[Close]

BLAST of Carg18817 vs. ExPASy TrEMBL
Match: A0A6J1DDL8 (thionin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019402 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.3e-37
Identity = 76/107 (71.03%), Postives = 91/107 (85.05%), Query Frame = 0


Query: 4   IVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT-DL 63
           ++++  +LSLVAG+STASFGKCYAKCF+VCAITPG+P+GTC  KCL DCLF A++ T D 
Sbjct: 6   LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 65

Query: 64  N-HLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           N  +DT YFCKLGCATSLCTKFSTK DPAEKKV SCV+SCS+ C+ A
Sbjct: 66  NDQIDTRYFCKLGCATSLCTKFSTKKDPAEKKVGSCVDSCSQKCINA 112

[Close]

BLAST of Carg18817 vs. ExPASy TrEMBL
Match: A0A5D3BCH5 (Thionin-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold85G00550 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 3.4e-26
Identity = 65/111 (58.56%), Postives = 78/111 (70.27%), Query Frame = 0


Query: 1   MKSIVVICFILSLVAGRST--ASFGKCYAKCFIVCAITPGVP-IGTCGGKCLADCLFPAS 60
           M+SIV+I FILSLVAGRST      K + KC   CAI P +P +  C  KC+ADC+  AS
Sbjct: 1   MRSIVLIHFILSLVAGRSTPVLDTAKWFVKCMAECAILPIIPVVSICAVKCMADCIPVAS 60

Query: 61  APTDLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 109
           +P DLN +DTHYFCKLGC T  CTK STK D  E KVE+C N+C +TC+KA
Sbjct: 61  SPMDLNRMDTHYFCKLGCTTYRCTKLSTKKDLVENKVETCGNTCDQTCMKA 111

[Close]

BLAST of Carg18817 vs. ExPASy TrEMBL
Match: A0A067E0U5 (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033731mg PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.7e-25
Identity = 57/105 (54.29%), Postives = 76/105 (72.38%), Query Frame = 0


Query: 3   SIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPTDL 62
           S++++  +  L+ G+S A+F +CY  CFI+C ITPG  +G+C  KCL DC+FP    T L
Sbjct: 8   SLLMVYLMTGLLVGQSQAAFKECYETCFIICYITPGTTLGSCAAKCLKDCIFPPKLHT-L 67

Query: 63  NHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLK 108
           +  DT YFCKLGCATSLCT  S+K +PA +KVE CVNSCS++C K
Sbjct: 68  SLQDTEYFCKLGCATSLCTNLSSKDNPAGEKVEGCVNSCSKSCSK 111

[Close]

BLAST of Carg18817 vs. TAIR 10
Match: AT1G12663.1 (Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant thionin (PR-13) family with the following members: At1g66100, At5g36910, At1g72260, At2g15010, At1g12663, At1g12660. )

HSP 1 Score: 60.8 bits (146), Expect = 7.5e-10
Identity = 37/110 (33.64%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 3   SIVVICFILSLVAGRSTAS--FGKCYAKCFIVCAITPGVP-IGTCGGKCLADCLFPASAP 62
           +++++  I +L+A  +     F +CY  C + C      P    C   C  +CL   S P
Sbjct: 4   AVMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPP 63

Query: 63  T-DLNHLD-THYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLK 108
           +   N++D + YFCKLGCAT  C   S+  +P  ++V +CV+SCS  C K
Sbjct: 64  SVSSNNIDESDYFCKLGCATYHCVSLSSIQNPNVERVSACVDSCSNKCTK 113

[Close]

BLAST of Carg18817 vs. TAIR 10
Match: AT1G12672.2 (unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12663.1). )

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-07
Identity = 27/62 (43.55%), Postives = 35/62 (56.45%), Query Frame = 0


Query: 48  CLADCLFPASAPTD----LNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSR 106
           C+  CL P S+  D    +NH D  Y+CKLGC+T  C   S+  +P   KV  CV+SCS 
Sbjct: 41  CIKTCLEPPSSQMDSTNEINHND--YYCKLGCSTHHCASLSSIQNPNVDKVVDCVDSCSD 100

[Close]

BLAST of Carg18817 vs. TAIR 10
Match: AT1G12672.1 (unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12663.1); Has 4 Blast hits to 4 proteins in 1 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 4; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 45.8 bits (107), Expect = 2.5e-05
Identity = 21/45 (46.67%), Postives = 28/45 (62.22%), Query Frame = 0


Query: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTC 106
           ++NH D  Y+CKLGC+T  C   S+  +P   KV  CV+SCS  C
Sbjct: 20  EINHND--YYCKLGCSTHHCASLSSIQNPNVDKVVDCVDSCSDKC 62

The following BLAST results are available for this feature:

BLAST of Carg18817 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025781.1	6.5e-56	100.00	Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6593441.1	3.2e-55	99.07	Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023000407.1	6.1e-54	97.22	thionin-like protein 2 [Cucurbita maxima]	[more]
XP_038899787.1	1.0e-48	84.26	thionin-like protein 2 [Benincasa hispida]	[more]
KAG7014098.1	1.3e-43	77.78	Thionin-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Carg18817 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8MRP4	1.1e-08	33.64	Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1	[more]

BLAST of Carg18817 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KDK0	3.0e-54	97.22	thionin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111494667 PE=4 SV=1	[more]
A0A0A0KC18	7.1e-40	78.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446640 PE=4 SV=1	[more]
A0A6J1DDL8	4.3e-37	71.03	thionin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019402 PE=4 SV=1	[more]
A0A5D3BCH5	3.4e-26	58.56	Thionin-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold8...	[more]
A0A067E0U5	1.7e-25	54.29	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033731mg PE=4 SV=1	[more]

BLAST of Carg18817 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12663.1	7.5e-10	33.64	Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant t...	[more]
AT1G12672.2	3.5e-07	43.55	unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana prot...	[more]
AT1G12672.1	2.5e-05	46.67	unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana prot...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR36312:SF1	THIONIN-LIKE PROTEIN 1	coord: 2..107
IPR038975	Thionin-like protein	PANTHER	PTHR36312	THIONIN-LIKE PROTEIN 1	coord: 2..107

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18817-RA	Carg18817-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane