Carg18752 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18752
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein CHUP1, chloroplastic isoform X1
LocationCarg_Chr08: 4045387 .. 4047664 (+)
RNA-Seq ExpressionCarg18752
SyntenyCarg18752
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGAGGAGAAGACGGATTTGGTGAAGCCTGTGTTGTTCAAATTTGGTGTTGTTCTGGCTATCTCTTTTGCTAGTTTTATGTATTCCCGCTTTAGAATCAGGAATAAAAGACCTTCTCTGGCTCCTCCCTCCTCCAGTTCTTCAGGTTCTTCCATTCAATCTCTTATGCTTCATCAATATATTCTTGAATCACATATTTCACTTGCTTTTAGCAGATGAATGGGGTAATAATAAAGTTGAGTTGGGAACAGGAAGAGGCCATAAACTTGATGATCAAACAATGAAGGTAGCTACAGCAGCAGCAGCATCCTCTAATGCTATTATTCTTGCAGCTGATGCCTATGTGAGTTTGCTGAATTTTGGTAATATGAACAACTTAGAATGTTCAAGAAGTTCAGAAACCAAATTTAACCCATTGACATAGGCGATGATTTGTTTTCAGCATGTTGGTCTGTGATTATAGACATAAGCTGGCTTCATGTTATGTGTTTGATATCTTTCTCAGGAAGAAATGTGTATTCAGAAAGCCAATGGTGATGATTCAAGTGCTGGTTTCTCCACTGGAAATGATCACATTGTAGATGAAGAAGGGTTGCTTCTCCCAGAGTTTCAGGAACTTGTCAAGCAATTTGATCTTTCTGCAGCAAATGCTGGGTTTTCTCCTAAGAAAAATGCTGGAGCACTGAGGTTGGGGATCGAAACTCCGAAAGCCTATAAAAGGGTCGAGTCGGATGGATATGAACATGAGATCAAACACCTGAAAAGCAAGGTGAAAATGCTTCGAGAAAGGGAGAGAAATCTTGAGGTTCAGTTACTTGAGTATTATGGTCTGAAAGAGCAAGAAACTGCTGTCATGGAGCTCCAAAATAGGCTGAAGATTAACAACATGGAGGCTAAACTTTTCACTCTGAAGATTGAGTCCCTTCAGGCGGATAATCGACGATTAGAATCACAAGTTTCCGACCAAGCAAAATCAGCATCCGACCTCGAGGCTGCAAGAACAACAATTAAGTTTCTCAAGAAAAAACTTAGACATGAAGCAGAACAGAACAGGGAACAGATCGTAAATCTTCAGCAAAGAGTTACTAAGCTGCTTGACCAAGAATGTAAGATAAATGAAAGCACTAAAAATGACCAAATCAAGCTGCAAAATATTGAAGATTTGGAGAAAGAGATCGAGGAGCTAAAGAAGGCGAATTCGAGATTGCAAAAAGAAAATTCTGATCTCGGTCGGAGACTAGATGCTACTCAGTTTCTTGCAAATTCTATCTTGGAAGACCAAGAAGTATGTTCTTATTGCTACTATAGCTATTTTACCATTATACAATTGTATGGTGACGAAAAATTTAATGCAATGCCCATCTAATTCATGCAGAAAGAATCACTTAAAGAAGAAAGGGACCGTTTCGCACAAGAAAACGAGACGTTGACGAAGGAAATCGAGCAGCTTCAAGCTCACCGTTGTGCAGATGTTGAAGAGCTAGTCTATCTCCGTTGGATTAATGCTTGCTTAAGATATGAACTGCGGAATTTTCAGCCTGCAGCAGGGAAAACAGCAGCAAGAGACCTAAGCAAAACATTAAGTCCTAAATCCGAGCATAAAGCGAAGAAGCTCATACTCGAATACGCAAATACAGAAGGAATTGAAGGGAAGAGCATTAACCTTACGGATTTTGATTCAGATCAGTGGTCATCCTCGCAAGCTTCCTCTCATACCGATCCTGGAGATTTGGATTATTCAGCTGTTGATTCTCGGTTAACAGCCAAACCAAGTTCAAACAAAATCAAATTCATGAGTAAACTCAGGAGTCTCTTGAGGGGAAAAAGTAATCAACAGAGCTCGGCTTTGTTACCAGAAAAATCTGCAGCAGCTGTAGGAGATGTTGATTCTCCACGTTACAGTTCAAGTCATTCTACTGGGACCAACGCTACTCGAGCCGATGGACATGGTACTGGATATACAACTCCATCTCAGAATTCATCAAGACGTTCAATGGATTTTCACAGGTTGAATAGCCAAAAGGAAGACGACGTAAAAACTGAGGATTCCCTTAGAAGGAACAGTGATGTTGGCTACATTAACAAGAGATTTGTTTCAGGCAGTGACAGATCGAGCAATTCGTTGTATAGATCTTCAAGTCAGGAAACAGAATCAACCGACAAGTCCGACAAATCTGAGAAGTCCGAGTTGTTGAAATATGCTGAAGTGTTGAAAAACAGCAGGGGAGATAAGAACCAATCTCGCCGGAAGGTTGCACCCATGTGTTCAATTTAA

mRNA sequence

ATGATGGAGGAGAAGACGGATTTGGTGAAGCCTGTGTTGTTCAAATTTGGTGTTGTTCTGGCTATCTCTTTTGCTAGTTTTATGTATTCCCGCTTTAGAATCAGGAATAAAAGACCTTCTCTGGCTCCTCCCTCCTCCAGTTCTTCAGATGAATGGGGTAATAATAAAGTTGAGTTGGGAACAGGAAGAGGCCATAAACTTGATGATCAAACAATGAAGGTAGCTACAGCAGCAGCAGCATCCTCTAATGCTATTATTCTTGCAGCTGATGCCTATGAAGAAATGTGTATTCAGAAAGCCAATGGTGATGATTCAAGTGCTGGTTTCTCCACTGGAAATGATCACATTGTAGATGAAGAAGGGTTGCTTCTCCCAGAGTTTCAGGAACTTGTCAAGCAATTTGATCTTTCTGCAGCAAATGCTGGGTTTTCTCCTAAGAAAAATGCTGGAGCACTGAGGTTGGGGATCGAAACTCCGAAAGCCTATAAAAGGGTCGAGTCGGATGGATATGAACATGAGATCAAACACCTGAAAAGCAAGGTGAAAATGCTTCGAGAAAGGGAGAGAAATCTTGAGGTTCAGTTACTTGAGTATTATGGTCTGAAAGAGCAAGAAACTGCTGTCATGGAGCTCCAAAATAGGCTGAAGATTAACAACATGGAGGCTAAACTTTTCACTCTGAAGATTGAGTCCCTTCAGGCGGATAATCGACGATTAGAATCACAAGTTTCCGACCAAGCAAAATCAGCATCCGACCTCGAGGCTGCAAGAACAACAATTAAGTTTCTCAAGAAAAAACTTAGACATGAAGCAGAACAGAACAGGGAACAGATCGTAAATCTTCAGCAAAGAGTTACTAAGCTGCTTGACCAAGAATGTAAGATAAATGAAAGCACTAAAAATGACCAAATCAAGCTGCAAAATATTGAAGATTTGGAGAAAGAGATCGAGGAGCTAAAGAAGGCGAATTCGAGATTGCAAAAAGAAAATTCTGATCTCGGTCGGAGACTAGATGCTACTCAGTTTCTTGCAAATTCTATCTTGGAAGACCAAGAAAAAGAATCACTTAAAGAAGAAAGGGACCGTTTCGCACAAGAAAACGAGACGTTGACGAAGGAAATCGAGCAGCTTCAAGCTCACCGTTGTGCAGATGTTGAAGAGCTAGTCTATCTCCGTTGGATTAATGCTTGCTTAAGATATGAACTGCGGAATTTTCAGCCTGCAGCAGGGAAAACAGCAGCAAGAGACCTAAGCAAAACATTAAGTCCTAAATCCGAGCATAAAGCGAAGAAGCTCATACTCGAATACGCAAATACAGAAGGAATTGAAGGGAAGAGCATTAACCTTACGGATTTTGATTCAGATCAGTGGTCATCCTCGCAAGCTTCCTCTCATACCGATCCTGGAGATTTGGATTATTCAGCTGTTGATTCTCGGTTAACAGCCAAACCAAGTTCAAACAAAATCAAATTCATGAGTAAACTCAGGAGTCTCTTGAGGGGAAAAAGTAATCAACAGAGCTCGGCTTTGTTACCAGAAAAATCTGCAGCAGCTGTAGGAGATGTTGATTCTCCACGTTACAGTTCAAGTCATTCTACTGGGACCAACGCTACTCGAGCCGATGGACATGGTACTGGATATACAACTCCATCTCAGAATTCATCAAGACGTTCAATGGATTTTCACAGGTTGAATAGCCAAAAGGAAGACGACGTAAAAACTGAGGATTCCCTTAGAAGGAACAGTGATGTTGGCTACATTAACAAGAGATTTGTTTCAGGCAGTGACAGATCGAGCAATTCGTTGTATAGATCTTCAAGTCAGGAAACAGAATCAACCGACAAGTCCGACAAATCTGAGAAGTCCGAGTTGTTGAAATATGCTGAAGTGTTGAAAAACAGCAGGGGAGATAAGAACCAATCTCGCCGGAAGGTTGCACCCATGTGTTCAATTTAA

Coding sequence (CDS)

ATGATGGAGGAGAAGACGGATTTGGTGAAGCCTGTGTTGTTCAAATTTGGTGTTGTTCTGGCTATCTCTTTTGCTAGTTTTATGTATTCCCGCTTTAGAATCAGGAATAAAAGACCTTCTCTGGCTCCTCCCTCCTCCAGTTCTTCAGATGAATGGGGTAATAATAAAGTTGAGTTGGGAACAGGAAGAGGCCATAAACTTGATGATCAAACAATGAAGGTAGCTACAGCAGCAGCAGCATCCTCTAATGCTATTATTCTTGCAGCTGATGCCTATGAAGAAATGTGTATTCAGAAAGCCAATGGTGATGATTCAAGTGCTGGTTTCTCCACTGGAAATGATCACATTGTAGATGAAGAAGGGTTGCTTCTCCCAGAGTTTCAGGAACTTGTCAAGCAATTTGATCTTTCTGCAGCAAATGCTGGGTTTTCTCCTAAGAAAAATGCTGGAGCACTGAGGTTGGGGATCGAAACTCCGAAAGCCTATAAAAGGGTCGAGTCGGATGGATATGAACATGAGATCAAACACCTGAAAAGCAAGGTGAAAATGCTTCGAGAAAGGGAGAGAAATCTTGAGGTTCAGTTACTTGAGTATTATGGTCTGAAAGAGCAAGAAACTGCTGTCATGGAGCTCCAAAATAGGCTGAAGATTAACAACATGGAGGCTAAACTTTTCACTCTGAAGATTGAGTCCCTTCAGGCGGATAATCGACGATTAGAATCACAAGTTTCCGACCAAGCAAAATCAGCATCCGACCTCGAGGCTGCAAGAACAACAATTAAGTTTCTCAAGAAAAAACTTAGACATGAAGCAGAACAGAACAGGGAACAGATCGTAAATCTTCAGCAAAGAGTTACTAAGCTGCTTGACCAAGAATGTAAGATAAATGAAAGCACTAAAAATGACCAAATCAAGCTGCAAAATATTGAAGATTTGGAGAAAGAGATCGAGGAGCTAAAGAAGGCGAATTCGAGATTGCAAAAAGAAAATTCTGATCTCGGTCGGAGACTAGATGCTACTCAGTTTCTTGCAAATTCTATCTTGGAAGACCAAGAAAAAGAATCACTTAAAGAAGAAAGGGACCGTTTCGCACAAGAAAACGAGACGTTGACGAAGGAAATCGAGCAGCTTCAAGCTCACCGTTGTGCAGATGTTGAAGAGCTAGTCTATCTCCGTTGGATTAATGCTTGCTTAAGATATGAACTGCGGAATTTTCAGCCTGCAGCAGGGAAAACAGCAGCAAGAGACCTAAGCAAAACATTAAGTCCTAAATCCGAGCATAAAGCGAAGAAGCTCATACTCGAATACGCAAATACAGAAGGAATTGAAGGGAAGAGCATTAACCTTACGGATTTTGATTCAGATCAGTGGTCATCCTCGCAAGCTTCCTCTCATACCGATCCTGGAGATTTGGATTATTCAGCTGTTGATTCTCGGTTAACAGCCAAACCAAGTTCAAACAAAATCAAATTCATGAGTAAACTCAGGAGTCTCTTGAGGGGAAAAAGTAATCAACAGAGCTCGGCTTTGTTACCAGAAAAATCTGCAGCAGCTGTAGGAGATGTTGATTCTCCACGTTACAGTTCAAGTCATTCTACTGGGACCAACGCTACTCGAGCCGATGGACATGGTACTGGATATACAACTCCATCTCAGAATTCATCAAGACGTTCAATGGATTTTCACAGGTTGAATAGCCAAAAGGAAGACGACGTAAAAACTGAGGATTCCCTTAGAAGGAACAGTGATGTTGGCTACATTAACAAGAGATTTGTTTCAGGCAGTGACAGATCGAGCAATTCGTTGTATAGATCTTCAAGTCAGGAAACAGAATCAACCGACAAGTCCGACAAATCTGAGAAGTCCGAGTTGTTGAAATATGCTGAAGTGTTGAAAAACAGCAGGGGAGATAAGAACCAATCTCGCCGGAAGGTTGCACCCATGTGTTCAATTTAA

Protein sequence

MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELGTGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEEGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTKNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEERDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKTLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRLTAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRADGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSNSLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Homology
BLAST of Carg18752 vs. NCBI nr
Match: KAG7025844.1 (Protein CHUP1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 651/651 (100.00%), Postives = 651/651 (100.00%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120
           TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE
Sbjct: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120

Query: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180
           GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK
Sbjct: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180

Query: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240
           VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE
Sbjct: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240

Query: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300
           SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK
Sbjct: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300

Query: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360
           NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER
Sbjct: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360

Query: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420
           DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT
Sbjct: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420

Query: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480
           LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL
Sbjct: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480

Query: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540
           TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA
Sbjct: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540

Query: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600
           DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN
Sbjct: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600

Query: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Sbjct: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 651

BLAST of Carg18752 vs. NCBI nr
Match: XP_023514513.1 (protein CHUP1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 642/651 (98.62%), Postives = 647/651 (99.39%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDL+KPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG
Sbjct: 1   MMEEKTDLMKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120
           TGRGHKLDDQTMKVAT AAASSNAIILAADAYEEMCIQKANGDDS+AGFSTGNDHIVDEE
Sbjct: 61  TGRGHKLDDQTMKVAT-AAASSNAIILAADAYEEMCIQKANGDDSNAGFSTGNDHIVDEE 120

Query: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180
           GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK
Sbjct: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180

Query: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240
           VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE
Sbjct: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240

Query: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300
           SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK
Sbjct: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300

Query: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360
           N QIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER
Sbjct: 301 NAQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360

Query: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420
           DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT
Sbjct: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420

Query: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480
           LSPKSEHKAKKLIL+YANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL
Sbjct: 421 LSPKSEHKAKKLILKYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480

Query: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540
           TAKPSSNKIKFMSKLRSLLRGKSNQQSSALL EKSAAAVGDVDSPRYSSSHSTGTNATRA
Sbjct: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLAEKSAAAVGDVDSPRYSSSHSTGTNATRA 540

Query: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600
           DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN
Sbjct: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600

Query: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           SLYRSSSQET+ +DKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Sbjct: 601 SLYRSSSQETDKSDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 650

BLAST of Carg18752 vs. NCBI nr
Match: XP_022964169.1 (protein CHUP1, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1183.7 bits (3061), Expect = 0.0e+00
Identity = 644/651 (98.92%), Postives = 644/651 (98.92%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEW NNKVELG
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWRNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120
            GRGHKLDDQTMKVAT  AASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE
Sbjct: 61  RGRGHKLDDQTMKVAT--AASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120

Query: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180
           GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK
Sbjct: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180

Query: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240
           VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE
Sbjct: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240

Query: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300
           SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK
Sbjct: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300

Query: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360
           NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER
Sbjct: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360

Query: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420
           DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT
Sbjct: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420

Query: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480
           LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL
Sbjct: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480

Query: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540
           TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA
Sbjct: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540

Query: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600
           DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN
Sbjct: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600

Query: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           SLYRSSSQETEST   DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Sbjct: 601 SLYRSSSQETEST---DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 646

BLAST of Carg18752 vs. NCBI nr
Match: KAG6593496.1 (Protein CHUP1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 639/651 (98.16%), Postives = 639/651 (98.16%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSS           
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSS----------- 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120
            GRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE
Sbjct: 61  -GRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120

Query: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180
           GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK
Sbjct: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180

Query: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240
           VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE
Sbjct: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240

Query: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300
           SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK
Sbjct: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300

Query: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360
           NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER
Sbjct: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360

Query: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420
           DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT
Sbjct: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420

Query: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480
           LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL
Sbjct: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480

Query: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540
           TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA
Sbjct: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540

Query: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600
           DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN
Sbjct: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600

Query: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Sbjct: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 639

BLAST of Carg18752 vs. NCBI nr
Match: XP_023000341.1 (protein CHUP1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 632/652 (96.93%), Postives = 640/652 (98.16%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDS-SAGFSTGNDHIVDE 120
           TGRGHKLDDQTMKVAT  AASSNAIILAADAYEEMCIQKANGDDS +AGFSTGNDHIVD+
Sbjct: 61  TGRGHKLDDQTMKVAT--AASSNAIILAADAYEEMCIQKANGDDSNAAGFSTGNDHIVDK 120

Query: 121 EGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKS 180
           EG+LLPEFQELVKQFDLSAANAGFSPKKNAG LR GIETPKAYKRVE+DGYEHEIKHLKS
Sbjct: 121 EGMLLPEFQELVKQFDLSAANAGFSPKKNAGELRSGIETPKAYKRVETDGYEHEIKHLKS 180

Query: 181 KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL 240
           KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL
Sbjct: 181 KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL 240

Query: 241 ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINEST 300
           ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQE KINEST
Sbjct: 241 ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQEYKINEST 300

Query: 301 KNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE 360
           KNDQIKLQNIEDLEKEIEELKK NSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE
Sbjct: 301 KNDQIKLQNIEDLEKEIEELKKVNSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE 360

Query: 361 RDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK 420
           RD FAQEN+TLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK
Sbjct: 361 RDCFAQENKTLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK 420

Query: 421 TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR 480
           TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR
Sbjct: 421 TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR 480

Query: 481 LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATR 540
           LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALL EKSAAAVGDVDSPRYSSSHSTGTN TR
Sbjct: 481 LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALLAEKSAAAVGDVDSPRYSSSHSTGTNTTR 540

Query: 541 ADGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSS 600
           ADGH TGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNS+VGYINKRFVSGSDRSS
Sbjct: 541 ADGHCTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSNVGYINKRFVSGSDRSS 600

Query: 601 NSLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           NSLYRSSSQETESTDKS+KSEKSELLKYAEVLKN+RGDKNQ RRKVAPMCSI
Sbjct: 601 NSLYRSSSQETESTDKSNKSEKSELLKYAEVLKNTRGDKNQPRRKVAPMCSI 650

BLAST of Carg18752 vs. ExPASy Swiss-Prot
Match: Q9LI74 (Protein CHUP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHUP1 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 7.2e-52
Identity = 154/401 (38.40%), Postives = 238/401 (59.35%), Query Frame = 0

Query: 113 NDHIVDEEGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEH 172
           +D++ D+   +LPEF++L     LS       P  +    +   E     +   +DG   
Sbjct: 81  SDYLDDD---ILPEFEDL-----LSGEIEYPLPDDDNNLEKAEKERKYEVEMAYNDG--- 140

Query: 173 EIKHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESL 232
           E++ LK  VK L ERE  LE +LLEYYGLKEQE+ ++ELQ +LKI  +E  +  + I SL
Sbjct: 141 ELERLKQLVKELEEREVKLEGELLEYYGLKEQESDIVELQRQLKIKTVEIDMLNITINSL 200

Query: 233 QADNRRLESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKL-LDQ 292
           QA+ ++L+ ++S       +LE AR  IK L+++++ +A Q + Q++ L+Q V+ L + +
Sbjct: 201 QAERKKLQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKE 260

Query: 293 ECKINESTKNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQ---FLANSIL 352
           E  +N+ T+ ++ KL+ ++DLE ++ ELK+ N  LQ E  +L  +LD+ +      +++ 
Sbjct: 261 EEAMNKDTEVER-KLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMT 320

Query: 353 EDQEKESLKEERDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPA 412
           E  +   ++EE +     NE L K++E LQ +R ++VEELVYLRW+NACLRYELRN+Q  
Sbjct: 321 ESDKVAKVREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTP 380

Query: 413 AGKTAARDLSKTLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDP 472
           AGK +ARDLSK LSPKS+ KAK+L+LEYA +E  +G        D+D  S+    S    
Sbjct: 381 AGKISARDLSKNLSPKSQAKAKRLMLEYAGSERGQG--------DTDLESNYSQPSSPGS 440

Query: 473 GDLDYSAVDSRLTAKPS-SNKIKFMSKLRSLLRGKSNQQSS 509
            D D +++DS  +   S S K   + KL+    GKS   SS
Sbjct: 441 DDFDNASMDSSTSRFSSFSKKPGLIQKLKKW--GKSKDDSS 459

BLAST of Carg18752 vs. ExPASy TrEMBL
Match: A0A6J1HMC2 (protein CHUP1, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464273 PE=4 SV=1)

HSP 1 Score: 1183.7 bits (3061), Expect = 0.0e+00
Identity = 644/651 (98.92%), Postives = 644/651 (98.92%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEW NNKVELG
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWRNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120
            GRGHKLDDQTMKVAT  AASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE
Sbjct: 61  RGRGHKLDDQTMKVAT--AASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120

Query: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180
           GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK
Sbjct: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180

Query: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240
           VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE
Sbjct: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240

Query: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300
           SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK
Sbjct: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300

Query: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360
           NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER
Sbjct: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360

Query: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420
           DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT
Sbjct: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420

Query: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480
           LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL
Sbjct: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480

Query: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540
           TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA
Sbjct: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540

Query: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600
           DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN
Sbjct: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600

Query: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           SLYRSSSQETEST   DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Sbjct: 601 SLYRSSSQETEST---DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 646

BLAST of Carg18752 vs. ExPASy TrEMBL
Match: A0A6J1KFL7 (protein CHUP1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494600 PE=4 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 632/652 (96.93%), Postives = 640/652 (98.16%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDS-SAGFSTGNDHIVDE 120
           TGRGHKLDDQTMKVAT  AASSNAIILAADAYEEMCIQKANGDDS +AGFSTGNDHIVD+
Sbjct: 61  TGRGHKLDDQTMKVAT--AASSNAIILAADAYEEMCIQKANGDDSNAAGFSTGNDHIVDK 120

Query: 121 EGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKS 180
           EG+LLPEFQELVKQFDLSAANAGFSPKKNAG LR GIETPKAYKRVE+DGYEHEIKHLKS
Sbjct: 121 EGMLLPEFQELVKQFDLSAANAGFSPKKNAGELRSGIETPKAYKRVETDGYEHEIKHLKS 180

Query: 181 KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL 240
           KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL
Sbjct: 181 KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL 240

Query: 241 ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINEST 300
           ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQE KINEST
Sbjct: 241 ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQEYKINEST 300

Query: 301 KNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE 360
           KNDQIKLQNIEDLEKEIEELKK NSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE
Sbjct: 301 KNDQIKLQNIEDLEKEIEELKKVNSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE 360

Query: 361 RDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK 420
           RD FAQEN+TLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK
Sbjct: 361 RDCFAQENKTLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK 420

Query: 421 TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR 480
           TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR
Sbjct: 421 TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR 480

Query: 481 LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATR 540
           LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALL EKSAAAVGDVDSPRYSSSHSTGTN TR
Sbjct: 481 LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALLAEKSAAAVGDVDSPRYSSSHSTGTNTTR 540

Query: 541 ADGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSS 600
           ADGH TGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNS+VGYINKRFVSGSDRSS
Sbjct: 541 ADGHCTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSNVGYINKRFVSGSDRSS 600

Query: 601 NSLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           NSLYRSSSQETESTDKS+KSEKSELLKYAEVLKN+RGDKNQ RRKVAPMCSI
Sbjct: 601 NSLYRSSSQETESTDKSNKSEKSELLKYAEVLKNTRGDKNQPRRKVAPMCSI 650

BLAST of Carg18752 vs. ExPASy TrEMBL
Match: A0A6J1HK30 (protein CHUP1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464273 PE=4 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 634/651 (97.39%), Postives = 634/651 (97.39%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSS           
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSS----------- 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120
            GRGHKLDDQTMKVAT  AASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE
Sbjct: 61  -GRGHKLDDQTMKVAT--AASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120

Query: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180
           GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK
Sbjct: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180

Query: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240
           VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE
Sbjct: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240

Query: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300
           SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK
Sbjct: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300

Query: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360
           NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER
Sbjct: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360

Query: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420
           DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT
Sbjct: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420

Query: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480
           LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL
Sbjct: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480

Query: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540
           TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA
Sbjct: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540

Query: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600
           DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN
Sbjct: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600

Query: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           SLYRSSSQETEST   DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Sbjct: 601 SLYRSSSQETEST---DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 634

BLAST of Carg18752 vs. ExPASy TrEMBL
Match: A0A6J1HI69 (protein CHUP1, chloroplastic isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111464273 PE=4 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 627/651 (96.31%), Postives = 627/651 (96.31%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEW NNKVELG
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWRNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEE 120
            GRGHKLDDQTMK                   EEMCIQKANGDDSSAGFSTGNDHIVDEE
Sbjct: 61  RGRGHKLDDQTMK-------------------EEMCIQKANGDDSSAGFSTGNDHIVDEE 120

Query: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180
           GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK
Sbjct: 121 GLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSK 180

Query: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240
           VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE
Sbjct: 181 VKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLE 240

Query: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300
           SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK
Sbjct: 241 SQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTK 300

Query: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360
           NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER
Sbjct: 301 NDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEER 360

Query: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420
           DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT
Sbjct: 361 DRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKT 420

Query: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480
           LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL
Sbjct: 421 LSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSRL 480

Query: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540
           TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA
Sbjct: 481 TAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 540

Query: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600
           DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN
Sbjct: 541 DGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSSN 600

Query: 601 SLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           SLYRSSSQETEST   DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI
Sbjct: 601 SLYRSSSQETEST---DKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 629

BLAST of Carg18752 vs. ExPASy TrEMBL
Match: A0A6J1KMC6 (protein CHUP1, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111494600 PE=4 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 615/652 (94.33%), Postives = 623/652 (95.55%), Query Frame = 0

Query: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60
           MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG
Sbjct: 1   MMEEKTDLVKPVLFKFGVVLAISFASFMYSRFRIRNKRPSLAPPSSSSSDEWGNNKVELG 60

Query: 61  TGRGHKLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDS-SAGFSTGNDHIVDE 120
           TGRGHKLDDQTMK                   EEMCIQKANGDDS +AGFSTGNDHIVD+
Sbjct: 61  TGRGHKLDDQTMK-------------------EEMCIQKANGDDSNAAGFSTGNDHIVDK 120

Query: 121 EGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKS 180
           EG+LLPEFQELVKQFDLSAANAGFSPKKNAG LR GIETPKAYKRVE+DGYEHEIKHLKS
Sbjct: 121 EGMLLPEFQELVKQFDLSAANAGFSPKKNAGELRSGIETPKAYKRVETDGYEHEIKHLKS 180

Query: 181 KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL 240
           KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL
Sbjct: 181 KVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRL 240

Query: 241 ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINEST 300
           ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQE KINEST
Sbjct: 241 ESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQEYKINEST 300

Query: 301 KNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE 360
           KNDQIKLQNIEDLEKEIEELKK NSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE
Sbjct: 301 KNDQIKLQNIEDLEKEIEELKKVNSRLQKENSDLGRRLDATQFLANSILEDQEKESLKEE 360

Query: 361 RDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK 420
           RD FAQEN+TLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK
Sbjct: 361 RDCFAQENKTLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSK 420

Query: 421 TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR 480
           TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR
Sbjct: 421 TLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGDLDYSAVDSR 480

Query: 481 LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATR 540
           LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALL EKSAAAVGDVDSPRYSSSHSTGTN TR
Sbjct: 481 LTAKPSSNKIKFMSKLRSLLRGKSNQQSSALLAEKSAAAVGDVDSPRYSSSHSTGTNTTR 540

Query: 541 ADGHGTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSDVGYINKRFVSGSDRSS 600
           ADGH TGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNS+VGYINKRFVSGSDRSS
Sbjct: 541 ADGHCTGYTTPSQNSSRRSMDFHRLNSQKEDDVKTEDSLRRNSNVGYINKRFVSGSDRSS 600

Query: 601 NSLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVAPMCSI 652
           NSLYRSSSQETESTDKS+KSEKSELLKYAEVLKN+RGDKNQ RRKVAPMCSI
Sbjct: 601 NSLYRSSSQETESTDKSNKSEKSELLKYAEVLKNTRGDKNQPRRKVAPMCSI 633

BLAST of Carg18752 vs. TAIR 10
Match: AT1G52080.1 (actin binding protein family )

HSP 1 Score: 303.5 bits (776), Expect = 4.0e-82
Identity = 242/650 (37.23%), Postives = 347/650 (53.38%), Query Frame = 0

Query: 12  VLFKFGVVLAISFASFMYSRFRIRNKR--PSLAPPSSSSSD----EWGNNKVELGTGRGH 71
           ++ + G  LA+SFA F+++RFR   KR  P+L P    SSD    ++ N  ++       
Sbjct: 13  LVLQLGAALAVSFAGFLFARFRKNTKRIGPTLPPLPPHSSDNGYRDYSNKSIDRRDEGTE 72

Query: 72  KLDDQTMKVATAAAASSNAIILAADAYEEMCIQKANGDDSSAGFSTGNDHIVDEEGL-LL 131
           K D++T+                                   G S   +  +DE+ + LL
Sbjct: 73  KTDEETL----------------------------------IGVSPRRECDLDEKDVFLL 132

Query: 132 PEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIKHLKSKVKML 191
           PEF+E  K+ DL   +   +P+ +       I  P A+   E   +E+EI  L++ V+ L
Sbjct: 133 PEFEEEAKKLDLLVCDDCETPRSD-------ITAPLAFPSEEEADHENEINRLRNTVRAL 192

Query: 192 RERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLESQVS 251
           RERER LE +LLEYY LKEQ+   MEL++RLK+N ME K+F  KI+ LQA+N +L+++  
Sbjct: 193 RERERCLEDKLLEYYSLKEQQKIAMELRSRLKLNQMETKVFNFKIKKLQAENEKLKAECF 252

Query: 252 DQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKLLDQECKINESTKNDQI 311
           + +K   +L+ A++ ++ LKKKL    +Q+  QI++L+QRV +L ++E K          
Sbjct: 253 EHSKVLLELDMAKSQVQVLKKKLNINTQQHVAQILSLKQRVARLQEEEIKAVLPDLEADK 312

Query: 312 KLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILEDQEK-ESLKEERDRF 371
            +Q + DLE EI EL   N+RLQ EN +L  +L++ Q +ANS LE+ E+ E+L+E+ +R 
Sbjct: 313 MMQRLRDLESEINELTDTNTRLQFENFELSEKLESVQIIANSKLEEPEEIETLREDCNRL 372

Query: 372 AQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAGKTAARDLSKTLSP 431
             ENE L K++EQLQ  RC D+E+LVYLRWINACLRYELR +QP AGKT ARDLS TLSP
Sbjct: 373 RSENEELKKDVEQLQGDRCTDLEQLVYLRWINACLRYELRTYQPPAGKTVARDLSTTLSP 432

Query: 432 KSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSH--TDPGDLDYSAVDSRLT 491
            SE KAK+LILEYA++E          + D D+WSSSQ  S   TD   LD S+VD+   
Sbjct: 433 TSEEKAKQLILEYAHSE---------DNTDYDRWSSSQEESSMITDSMFLDDSSVDTLFA 492

Query: 492 AK-PSSNKIKFMSKLRSLLRGKSNQQSSALLPEKSAAAVGDVDSPRYSSSHSTGTNATRA 551
            K   + K K M KL  +L GK  + S                  R  SS  + +N    
Sbjct: 493 TKTKKTGKKKLMHKLMKILHGKDTKDS----------------KKRAGSSEPSSSN---- 552

Query: 552 DGHGTG-YTTPSQNSSRRSMDFHRL--NSQKEDDVKTE-DSLRRNSDVGYINKRFVSGSD 611
               TG ++TP Q  S  SMDF  L     +E+D K     LRR S+             
Sbjct: 553 ----TGVHSTPRQLRSTHSMDFQMLMRGKDEEEDFKNHIVMLRRKSEAA----------- 570

Query: 612 RSSNSLYRSSSQETESTDKSDKSEKSELLKYAEVLKNSRGDKNQSRRKVA 647
                   SS+   E   ++D++ K EL+K A+ L  SR  K   ++ V+
Sbjct: 613 -------GSSTYGEEHCLETDQNGKKELIKLADALTKSRSTKKLHKKSVS 570

BLAST of Carg18752 vs. TAIR 10
Match: AT3G25690.1 (Hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 206.8 bits (525), Expect = 5.1e-53
Identity = 154/401 (38.40%), Postives = 238/401 (59.35%), Query Frame = 0

Query: 113 NDHIVDEEGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEH 172
           +D++ D+   +LPEF++L     LS       P  +    +   E     +   +DG   
Sbjct: 81  SDYLDDD---ILPEFEDL-----LSGEIEYPLPDDDNNLEKAEKERKYEVEMAYNDG--- 140

Query: 173 EIKHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESL 232
           E++ LK  VK L ERE  LE +LLEYYGLKEQE+ ++ELQ +LKI  +E  +  + I SL
Sbjct: 141 ELERLKQLVKELEEREVKLEGELLEYYGLKEQESDIVELQRQLKIKTVEIDMLNITINSL 200

Query: 233 QADNRRLESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKL-LDQ 292
           QA+ ++L+ ++S       +LE AR  IK L+++++ +A Q + Q++ L+Q V+ L + +
Sbjct: 201 QAERKKLQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKE 260

Query: 293 ECKINESTKNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQ---FLANSIL 352
           E  +N+ T+ ++ KL+ ++DLE ++ ELK+ N  LQ E  +L  +LD+ +      +++ 
Sbjct: 261 EEAMNKDTEVER-KLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMT 320

Query: 353 EDQEKESLKEERDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPA 412
           E  +   ++EE +     NE L K++E LQ +R ++VEELVYLRW+NACLRYELRN+Q  
Sbjct: 321 ESDKVAKVREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTP 380

Query: 413 AGKTAARDLSKTLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDP 472
           AGK +ARDLSK LSPKS+ KAK+L+LEYA +E  +G        D+D  S+    S    
Sbjct: 381 AGKISARDLSKNLSPKSQAKAKRLMLEYAGSERGQG--------DTDLESNYSQPSSPGS 440

Query: 473 GDLDYSAVDSRLTAKPS-SNKIKFMSKLRSLLRGKSNQQSS 509
            D D +++DS  +   S S K   + KL+    GKS   SS
Sbjct: 441 DDFDNASMDSSTSRFSSFSKKPGLIQKLKKW--GKSKDDSS 459

BLAST of Carg18752 vs. TAIR 10
Match: AT3G25690.2 (Hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 206.8 bits (525), Expect = 5.1e-53
Identity = 154/401 (38.40%), Postives = 238/401 (59.35%), Query Frame = 0

Query: 113 NDHIVDEEGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEH 172
           +D++ D+   +LPEF++L     LS       P  +    +   E     +   +DG   
Sbjct: 81  SDYLDDD---ILPEFEDL-----LSGEIEYPLPDDDNNLEKAEKERKYEVEMAYNDG--- 140

Query: 173 EIKHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESL 232
           E++ LK  VK L ERE  LE +LLEYYGLKEQE+ ++ELQ +LKI  +E  +  + I SL
Sbjct: 141 ELERLKQLVKELEEREVKLEGELLEYYGLKEQESDIVELQRQLKIKTVEIDMLNITINSL 200

Query: 233 QADNRRLESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKL-LDQ 292
           QA+ ++L+ ++S       +LE AR  IK L+++++ +A Q + Q++ L+Q V+ L + +
Sbjct: 201 QAERKKLQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKE 260

Query: 293 ECKINESTKNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQ---FLANSIL 352
           E  +N+ T+ ++ KL+ ++DLE ++ ELK+ N  LQ E  +L  +LD+ +      +++ 
Sbjct: 261 EEAMNKDTEVER-KLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMT 320

Query: 353 EDQEKESLKEERDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPA 412
           E  +   ++EE +     NE L K++E LQ +R ++VEELVYLRW+NACLRYELRN+Q  
Sbjct: 321 ESDKVAKVREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTP 380

Query: 413 AGKTAARDLSKTLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDP 472
           AGK +ARDLSK LSPKS+ KAK+L+LEYA +E  +G        D+D  S+    S    
Sbjct: 381 AGKISARDLSKNLSPKSQAKAKRLMLEYAGSERGQG--------DTDLESNYSQPSSPGS 440

Query: 473 GDLDYSAVDSRLTAKPS-SNKIKFMSKLRSLLRGKSNQQSS 509
            D D +++DS  +   S S K   + KL+    GKS   SS
Sbjct: 441 DDFDNASMDSSTSRFSSFSKKPGLIQKLKKW--GKSKDDSS 459

BLAST of Carg18752 vs. TAIR 10
Match: AT3G25690.3 (Hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 148.3 bits (373), Expect = 2.2e-35
Identity = 107/279 (38.35%), Postives = 170/279 (60.93%), Query Frame = 0

Query: 235 DNRRLESQVSDQAKSASDLEAARTTIKFLKKKLRHEAEQNREQIVNLQQRVTKL-LDQEC 294
           +++ L+ ++S       +LE AR  IK L+++++ +A Q + Q++ L+Q V+ L + +E 
Sbjct: 51  NDKNLQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKEEE 110

Query: 295 KINESTKNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQ---FLANSILED 354
            +N+ T+ ++ KL+ ++DLE ++ ELK+ N  LQ E  +L  +LD+ +      +++ E 
Sbjct: 111 AMNKDTEVER-KLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMTES 170

Query: 355 QEKESLKEERDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYELRNFQPAAG 414
            +   ++EE +     NE L K++E LQ +R ++VEELVYLRW+NACLRYELRN+Q  AG
Sbjct: 171 DKVAKVREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTPAG 230

Query: 415 KTAARDLSKTLSPKSEHKAKKLILEYANTEGIEGKSINLTDFDSDQWSSSQASSHTDPGD 474
           K +ARDLSK LSPKS+ KAK+L+LEYA +E  +G        D+D  S+    S     D
Sbjct: 231 KISARDLSKNLSPKSQAKAKRLMLEYAGSERGQG--------DTDLESNYSQPSSPGSDD 290

Query: 475 LDYSAVDSRLTAKPS-SNKIKFMSKLRSLLRGKSNQQSS 509
            D +++DS  +   S S K   + KL+    GKS   SS
Sbjct: 291 FDNASMDSSTSRFSSFSKKPGLIQKLKKW--GKSKDDSS 318

BLAST of Carg18752 vs. TAIR 10
Match: AT2G36650.1 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 51.2 bits (121), Expect = 3.6e-06
Identity = 64/292 (21.92%), Postives = 142/292 (48.63%), Query Frame = 0

Query: 116 IVDEEGLLLPEFQELVKQFDLSAANAGFSPKKNAGALRLGIETPKAYKRVESDGYEHEIK 175
           ++   GL+L  F  + +  D    ++  +P+ ++   R   E  +  +    +  + EI 
Sbjct: 25  VLSATGLILARF--VSRNEDNEVTSSTSNPESSSSPSR---ENDEEEEAESPNQQKQEIL 84

Query: 176 HLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQAD 235
            LKS+ + L+ +E  +E+    +  LK+QE  ++E ++ L +   +   F  ++ +++ +
Sbjct: 85  SLKSRFEELQRKEYEMELHFERFCNLKDQEVMLIEHKSILSLEKAQLDFFRKEVLAMEEE 144

Query: 236 NRRLESQVSDQAKSASDLEAARTTIKFLK---KKLRHEAEQNREQIVNLQQRVTKLLDQ- 295
           ++R ++ V    K   +++  R+    L+   KKLR +++Q   ++VN  +++  +  + 
Sbjct: 145 HKRGQALVIVYLKLVGEIQELRSENGLLEGKAKKLRRKSKQ-LYRVVNESRKIIGVEKEF 204

Query: 296 -ECKINESTKNDQIKLQNIEDLEKEIEELKKANSRLQKENSDLGRRLDATQFLANSILED 355
            +C     TKN+ +K     +LE ++++++     LQ+E  +L            S    
Sbjct: 205 LKCVDELETKNNIVK-----ELEGKVKDMEAYVDVLQEEKEEL---------FMKSSNST 264

Query: 356 QEKESLKEERDRFAQENETLTKEIEQLQAHRCADVEELVYLRWINACLRYEL 403
            E  S+++ R         + +E E+L+      V+E++ LRW NACLR+E+
Sbjct: 265 SEMVSVEDYR--------RIVEEYEELKKDYANGVKEVINLRWSNACLRHEV 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025844.10.0e+00100.00Protein CHUP1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_023514513.10.0e+0098.62protein CHUP1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022964169.10.0e+0098.92protein CHUP1, chloroplastic isoform X1 [Cucurbita moschata][more]
KAG6593496.10.0e+0098.16Protein CHUP1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023000341.10.0e+0096.93protein CHUP1, chloroplastic isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LI747.2e-5238.40Protein CHUP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHUP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HMC20.0e+0098.92protein CHUP1, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KFL70.0e+0096.93protein CHUP1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494... [more]
A0A6J1HK300.0e+0097.39protein CHUP1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1HI690.0e+0096.31protein CHUP1, chloroplastic isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KMC60.0e+0094.33protein CHUP1, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111494... [more]
Match NameE-valueIdentityDescription
AT1G52080.14.0e-8237.23actin binding protein family [more]
AT3G25690.15.1e-5338.40Hydroxyproline-rich glycoprotein family protein [more]
AT3G25690.25.1e-5338.40Hydroxyproline-rich glycoprotein family protein [more]
AT3G25690.32.2e-3538.35Hydroxyproline-rich glycoprotein family protein [more]
AT2G36650.13.6e-0621.92unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae ... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 353..380
NoneNo IPR availableCOILSCoilCoilcoord: 302..336
NoneNo IPR availableCOILSCoilCoilcoord: 201..291
NoneNo IPR availableCOILSCoilCoilcoord: 167..194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 610..642
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 591..651
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 525..560
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 592..609
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 503..579
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 561..579
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..64
NoneNo IPR availablePANTHERPTHR31342:SF4ACTIN BINDING PROTEIN FAMILYcoord: 1..644
IPR040265Protein CHUP1-likePANTHERPTHR31342PROTEIN CHUP1, CHLOROPLASTICcoord: 1..644

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18752-RACarg18752-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
cellular_component GO:0009707 chloroplast outer membrane
cellular_component GO:0016021 integral component of membrane