Carg18713 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18713
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Small heat shock protein HSP20
Location	Carg_Chr17: 7327854 .. 7328420 (+)
RNA-Seq Expression	Carg18713
Synteny	Carg18713

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTCAATGGAGACTCCTCCACCTCAAATTCCGACTCTCAGCCCACTTTTCCTCTTCATCTCTGCTTCTTCTTTCTCATCCTCTTCATGTTCTTGGCCTTCTCTTGGCACTCGACTTACGAGTCGGCTGTTGAAGGCGTTTTCGATCAGCTCAAGCTTCTTCTCATCGTCTCGCCACTCCTCCTCCTCCTCCTCCTTCACTGGCTCTCCAATGGTGAAAACGGCCGCCTACCGCCCCTCGTCTCCCTACCGGAAAAGGACTCGCTCCACCGCGCTGGAGGGACACCGTGGGGAGTTGGGTTTATGTTGGTTATTCTCCTTTTCATGATCTCTTACCAATCTTACTTCCAAGAACGGTGGTTTCCTCTGTTGAGCCGGTGATCGGATTGTATATCGGAAAGTCCAATCTCTTGTCTAGTTGTCGTTGAAGAATATTCTCTTGGGATTCTGTTTTTTTGAAGAACAAGAATTTAGGTAGAGATGTGTCGGATCTTGTCAGCATAATATTTGTTTATGTTGATCGTTAATGTAATTATTTTTTTTTTCTTTCTGATTTAGATATAA

mRNA sequence

ATGGCTGTCAATGGAGACTCCTCCACCTCAAATTCCGACTCTCAGCCCACTTTTCCTCTTCATCTCTGCTTCTTCTTTCTCATCCTCTTCATGTTCTTGGCCTTCTCTTGGCACTCGACTTACGAGTCGGCTGTTGAAGGCGTTTTCGATCAGCTCAAGCTTCTTCTCATCGTCTCGCCACTCCTCCTCCTCCTCCTCCTTCACTGGCTCTCCAATGGTGAAAACGGCCGCCTACCGCCCCTCGTCTCCCTACCGGAAAAGGACTCGCTCCACCGCGCTGGAGGGACACCGTGGGGAGTTGGGTTTATGTTGATATAA

Coding sequence (CDS)

ATGGCTGTCAATGGAGACTCCTCCACCTCAAATTCCGACTCTCAGCCCACTTTTCCTCTTCATCTCTGCTTCTTCTTTCTCATCCTCTTCATGTTCTTGGCCTTCTCTTGGCACTCGACTTACGAGTCGGCTGTTGAAGGCGTTTTCGATCAGCTCAAGCTTCTTCTCATCGTCTCGCCACTCCTCCTCCTCCTCCTCCTTCACTGGCTCTCCAATGGTGAAAACGGCCGCCTACCGCCCCTCGTCTCCCTACCGGAAAAGGACTCGCTCCACCGCGCTGGAGGGACACCGTGGGGAGTTGGGTTTATGTTGATATAA

Protein sequence

MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSPLLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI

Homology

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BLAST of Carg18713 vs. NCBI nr
Match: KAG7014204.1 (hypothetical protein SDJN02_24379, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 216.1 bits (549), Expect = 1.5e-52
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP
Sbjct: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI
Sbjct: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 105

[Close]

BLAST of Carg18713 vs. NCBI nr
Match: KAG6575655.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 214.5 bits (545), Expect = 4.3e-52
Identity = 103/105 (98.10%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP
Sbjct: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLL+HWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFML+
Sbjct: 61  LLLLLLVHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLV 105

[Close]

BLAST of Carg18713 vs. NCBI nr
Match: XP_022953918.1 (uncharacterized protein LOC111456323 [Cucurbita moschata])

HSP 1 Score: 213.8 bits (543), Expect = 7.3e-52
Identity = 103/105 (98.10%), Postives = 104/105 (99.05%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP
Sbjct: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLLLHWLSNGENGRLPPLV LPEKDSLHRAGGTPWGVGFML+
Sbjct: 61  LLLLLLLHWLSNGENGRLPPLVPLPEKDSLHRAGGTPWGVGFMLV 105

[Close]

BLAST of Carg18713 vs. NCBI nr
Match: XP_023548678.1 (uncharacterized protein LOC111807266 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 210.7 bits (535), Expect = 6.1e-51
Identity = 101/105 (96.19%), Postives = 103/105 (98.10%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAVNGDSS SNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP
Sbjct: 1   MAVNGDSSASNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLL+HWLSNGENGRLPPLV LPEKDSLHRAGGTPWGVGFML+
Sbjct: 61  LLLLLLVHWLSNGENGRLPPLVPLPEKDSLHRAGGTPWGVGFMLV 105

[Close]

BLAST of Carg18713 vs. NCBI nr
Match: XP_022991944.1 (uncharacterized protein LOC111488435 [Cucurbita maxima])

HSP 1 Score: 208.8 bits (530), Expect = 2.3e-50
Identity = 99/105 (94.29%), Postives = 104/105 (99.05%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAVNGDSST+NSDSQPTFPLHLCFFFLILFMFLAFSW+STYESAVEGVFDQLKLLLI+SP
Sbjct: 1   MAVNGDSSTTNSDSQPTFPLHLCFFFLILFMFLAFSWYSTYESAVEGVFDQLKLLLIISP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLL+HWLSNGENGRLPPLV LPEKDSLHRAGGTPWGVGFML+
Sbjct: 61  LLLLLLVHWLSNGENGRLPPLVPLPEKDSLHRAGGTPWGVGFMLV 105

[Close]

BLAST of Carg18713 vs. ExPASy TrEMBL
Match: A0A6J1GPN5 (uncharacterized protein LOC111456323 OS=Cucurbita moschata OX=3662 GN=LOC111456323 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.5e-52
Identity = 103/105 (98.10%), Postives = 104/105 (99.05%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP
Sbjct: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLLLHWLSNGENGRLPPLV LPEKDSLHRAGGTPWGVGFML+
Sbjct: 61  LLLLLLLHWLSNGENGRLPPLVPLPEKDSLHRAGGTPWGVGFMLV 105

[Close]

BLAST of Carg18713 vs. ExPASy TrEMBL
Match: A0A6J1JW83 (uncharacterized protein LOC111488435 OS=Cucurbita maxima OX=3661 GN=LOC111488435 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.1e-50
Identity = 99/105 (94.29%), Postives = 104/105 (99.05%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAVNGDSST+NSDSQPTFPLHLCFFFLILFMFLAFSW+STYESAVEGVFDQLKLLLI+SP
Sbjct: 1   MAVNGDSSTTNSDSQPTFPLHLCFFFLILFMFLAFSWYSTYESAVEGVFDQLKLLLIISP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLL+HWLSNGENGRLPPLV LPEKDSLHRAGGTPWGVGFML+
Sbjct: 61  LLLLLLVHWLSNGENGRLPPLVPLPEKDSLHRAGGTPWGVGFMLV 105

[Close]

BLAST of Carg18713 vs. ExPASy TrEMBL
Match: A0A6J1DCG1 (uncharacterized protein LOC111019747 OS=Momordica charantia OX=3673 GN=LOC111019747 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.9e-38
Identity = 80/105 (76.19%), Postives = 90/105 (85.71%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MAV  +S     +SQP+FPLHLCFFFLIL MFL FSW+S YESAV+GVFDQLKLLLI SP
Sbjct: 1   MAVQNNS----DESQPSFPLHLCFFFLILLMFLTFSWYSNYESAVDGVFDQLKLLLIASP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLL +HWLSN +NGRLPPL+ LPEKDSLHRAG TPWGVGF+L+
Sbjct: 61  LLLLLAVHWLSNADNGRLPPLLPLPEKDSLHRAGATPWGVGFLLV 101

[Close]

BLAST of Carg18713 vs. ExPASy TrEMBL
Match: A0A0A0K9W6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G429630 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.9e-38
Identity = 78/96 (81.25%), Postives = 88/96 (91.67%), Query Frame = 0


Query: 10  SNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSPLLLLLLLHW 69
           +N+ SQPTFPLHLCFFFLIL MFL+FSW+S YESAVE VFDQLKLLLIVSPLLLLLL+HW
Sbjct: 2   ANTHSQPTFPLHLCFFFLILLMFLSFSWYSNYESAVEDVFDQLKLLLIVSPLLLLLLVHW 61

Query: 70  LSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LSN ++G LP L+ LPEKDSLHRAGGTPWGVGF+L+
Sbjct: 62  LSNTKSGHLPSLIPLPEKDSLHRAGGTPWGVGFLLV 97

[Close]

BLAST of Carg18713 vs. ExPASy TrEMBL
Match: A0A6J1H718 (uncharacterized protein LOC111460692 OS=Cucurbita moschata OX=3662 GN=LOC111460692 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.2e-37
Identity = 81/105 (77.14%), Postives = 87/105 (82.86%), Query Frame = 0


Query: 1   MAVNGDSSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSP 60
           MA NG SS +NS SQPTFPLHLCFFFLIL MFL+FSW+S YES VEGVFDQLKLLLIVSP
Sbjct: 1   MAANGGSSAANSHSQPTFPLHLCFFFLILLMFLSFSWYSNYESVVEGVFDQLKLLLIVSP 60

Query: 61  LLLLLLLHWLSNGENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           LLLLLL+HWLSN  N        LPEKDSLH AGGTPWGVGF+L+
Sbjct: 61  LLLLLLVHWLSNAGN------FLLPEKDSLHHAGGTPWGVGFLLV 99

[Close]

BLAST of Carg18713 vs. TAIR 10
Match: AT2G31940.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 6 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G19875.1); Has 227 Blast hits to 227 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 227; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 106.3 bits (264), Expect = 1.5e-23
Identity = 51/97 (52.58%), Postives = 68/97 (70.10%), Query Frame = 0


Query: 11  NSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSPLLLLLLLHWL 70
           + +  P  PLHLC F LIL MF+  SW+++YE  +EG   Q KL L+ SPLLLLL +H+L
Sbjct: 4   DQEGSPPVPLHLCVFVLILLMFVTISWYASYEPVIEGFTYQFKLALMASPLLLLLAVHFL 63

Query: 71  SN--GENGRLPPLVSLPEKDSLHRAGGTPWGVGFMLI 106
           S+  G  G +  L+ L E++SL+RAGGTPWGV FML+
Sbjct: 64  SDDQGVGGMMTSLIHLNERESLYRAGGTPWGVAFMLV 100

[Close]

BLAST of Carg18713 vs. TAIR 10
Match: AT5G19875.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to oxidative stress; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G31940.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 87.4 bits (215), Expect = 7.3e-18
Identity = 41/85 (48.24%), Postives = 58/85 (68.24%), Query Frame = 0


Query: 21  HLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSPLLLLLLLHWLSNGENGRLPP 80
           HLCF F+++F  L+F+W+  +ES +E   D LKL+ I +PL LLLL+H+ S G    L  
Sbjct: 21  HLCFLFIVVFFVLSFTWYLNFESIIEDTLDHLKLVFIFTPLFLLLLVHFFSGG----LSF 80

Query: 81  LVSLPEKDSLHRAGGTPWGVGFMLI 106
            V  PE+DS+HRAG +PWGV  +L+
Sbjct: 81  YVPWPEQDSIHRAGSSPWGVAAVLV 101

[Close]

BLAST of Carg18713 vs. TAIR 10
Match: AT2G21180.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G19875.1); Has 124 Blast hits to 124 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 124; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 8.4e-06
Identity = 32/110 (29.09%), Postives = 50/110 (45.45%), Query Frame = 0


Query: 7   SSTSNSDSQPTFPLHLCFFFLILFMFLAFSWHSTYESAVEGVFDQLKLLLIVSPLLLLLL 66
           SS  +S S    P  +     +  +FL  SW+ + E A E   +Q+   L++ PL L++L
Sbjct: 8   SSILDSFSLSPLPYPVLLILAVASVFLLSSWYFSLEEAAESAEEQINFALLLIPLFLIVL 67

Query: 67  LHWLSNGENGRLPPLVSLPEKDSLHR-----------AGGTPWGVGFMLI 106
           + WLS+ EN    P   L    S  R            G +PWGV  +++
Sbjct: 68  VRWLSSMEN----PDALLGMFSSSRRTTYVSPGAGGDGGSSPWGVAALIV 113

The following BLAST results are available for this feature:

BLAST of Carg18713 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014204.1	1.5e-52	100.00	hypothetical protein SDJN02_24379, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6575655.1	4.3e-52	98.10	E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022953918.1	7.3e-52	98.10	uncharacterized protein LOC111456323 [Cucurbita moschata]	[more]
XP_023548678.1	6.1e-51	96.19	uncharacterized protein LOC111807266 [Cucurbita pepo subsp. pepo]	[more]
XP_022991944.1	2.3e-50	94.29	uncharacterized protein LOC111488435 [Cucurbita maxima]	[more]

BLAST of Carg18713 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg18713 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GPN5	3.5e-52	98.10	uncharacterized protein LOC111456323 OS=Cucurbita moschata OX=3662 GN=LOC1114563...	[more]
A0A6J1JW83	1.1e-50	94.29	uncharacterized protein LOC111488435 OS=Cucurbita maxima OX=3661 GN=LOC111488435...	[more]
A0A6J1DCG1	2.9e-38	76.19	uncharacterized protein LOC111019747 OS=Momordica charantia OX=3673 GN=LOC111019...	[more]
A0A0A0K9W6	4.9e-38	81.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G429630 PE=4 SV=1	[more]
A0A6J1H718	3.2e-37	77.14	uncharacterized protein LOC111460692 OS=Cucurbita moschata OX=3662 GN=LOC1114606...	[more]

BLAST of Carg18713 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31940.1	1.5e-23	52.58	unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: endomembra...	[more]
AT5G19875.1	7.3e-18	48.24	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response...	[more]
AT2G21180.1	8.4e-06	29.09	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33306	EXPRESSED PROTEIN-RELATED-RELATED	coord: 12..105
None	No IPR available	PANTHER	PTHR33306:SF5	EXPRESSED PROTEIN-RELATED	coord: 12..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18713-RA	Carg18713-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane