Carg18596 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18596
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome B5-like protein
Location	Carg_Chr12: 11749578 .. 11752042 (+)
RNA-Seq Expression	Carg18596
Synteny	Carg18596

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAATCCGATCCATATTCTTAAGGTAAGTTCTCAGTTTCGCATCGCTTAGCTAAATCATCTGCATCAAAACTAGCTTAAACCAGTGACCCGGATGGTCCGAGAATCTTTGCTGAATTTGTGGATTCTGGAGCATTTTCTTGCTTACGAAACAAAATTATTATTTCTTAGTTTCAATTTCGGAATGGTAATGCTTCGTTGCAGTACTATGCTTTTGCATTTATTGTGAATCTGATTTAGCAGTGAGCTATATAAAATGATGATCATTGTAGCCGCATTGGTTCTCGGTGTGTTGTTTGGCGCTCTGATTTTAATCCCACGCGACCGTAAGTTTGGTGAAGTGTATTGTTTCAGTTCTTTAATCCTTTTATGCATAAATTCACATCGATTTGAACAAACAATGCTCTTATTTTCTGGATGCAGGAAATGCACAAAAGGTTCAATTAAATAATATCAACAAGGTAAATTTGGACTTGTTTTCCTTTAGTTTCAATTTTTAAATGAATTAAGCAACCCTTTGCCCGCTTAGAACTGGAACTTAAACATGAGTAAATCATCATGGTTTGTGCAATTTTCTATTTCCAGATTTCAACTATTGAGAATGTATTGCTTTTCCATTGGATCCGACTAAAATTAGGATTTGTTGAGGATCGTTGGGAGAGAGTCCCACAGTGACTAATTTAGGGAATGATCATGGGTTTATAAGTAAGGAATACATCTCTATTGGTATGAGGTCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAACTTATGCTCTGGATAATATCATATCATTGTGGTGTGGAGAGTCGTAATTTCTAACCGGATTTAAAAATAGAAATGGAAATAGAACATTCCAAGAGATTTAGTGAAACTGAAACAATTTGTACAGTTTTCTTTTTCGAGCTTATCTGTTGATGCTGCAAACTTTAGTGATGGGGCTTATTTGTGTTCAATTAAAGGATCTGCGTTGATATTTCATACAGGAAGAAGTGTTCTTTGTGGTTTCTCATTATATTCTAAATGTTTTTTCTTGCTTATAGGCATCTAAAGTGTACAGTAAAGACGAGGTTTCGGTTCATAACAAGAGAACTGATTGCTGGATCATTATCAAGAACAAGGTATTATCGGATGTCTAAGCTCGTAGCAAAACTTGGGGTTTGCTTGTATGTTGTCATACTCGTAATCTATTTTTCGATTTTTATCGAGCATAGGTGTACGATGTTACGTCCTATGTTGAAGAACATCCAGGAGGAGATGCTATCCTAGCACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGTAAGCCAATCTGACACCTCCTCTCTCAATCATTGTTATATCATAATAATTTGATCTGTCCAAATAATCTGGATTGGATACTCTTATCATTTTTTTAGCTTTGATAAACAATAATTAAGATCAGCAAAGCTTTTTCAAAATGGTTGCTTCTGCGATCCTTTTTTCCCTGTTTTGTATGCAAACTACAGAATCATGGTGTAAATGGATAGCACTTGGATATGTCAATGGTGGTATATTGATTTTCTTTAAACAACACGTGGGCGCTTTAGTTTGGTTAGCATGTTCATATCACACACTCTGTCACTTGTTGAACATATATCAAACATTTGTTAGATACATATCGATCATTTGTTATGTATACTAAATAGATATATAATAATAAGTTTTAAGAAATGCACAAACTTACGGACTTTGAATTCTCATATATATTTGAATAAATGCGTCCATGTTGTTTATGACGTGCATAATGTGCGTCATGTCGTATAAGTGTCTTATTTGTATATGTGCTTTTTAGCATGTGGAGGTGAAGATTCAAACGCATTTGACTTTGTGATCGATGATATATGTTTTAACCAATTGAGCTATGCTCAAGTTGAGCTCAAAGATTCTATATATAAAAAAGCGGTCATGTTGGCTTTACTCAAAATTTTACCTTTCTTTCTTCCATCGTTACAGCAATCTTCCGAACACGTTTATTTCAACCTCAATATTTTTTTGTGCAGGCCACAACATGCAACTCGAGTCTTCGACATGATCGACGACTTCTATGTTGGAGATCTGAGGCTGTAATATCCAAGAAACATCTGGCTTTGCGCTATCACGTAGAGGACGTATTTCGCAGCGTTCCAGCTCTAGAGATGTCTTTCAGGGAATATATCAAATGATTTGAATTGTCTGGTTAGCATTCTTGCGATGTTGTAACATTTGTAGCTGTACACTTTCATTTAGATCTTCAACTTTCTAGTTTTATCTCTTTAGTTTGAATTCTTCTTTCTTTTTTTTATGATTGCAAGAGGAGAGAAACTTTTCATAGTGGTCTATTTTGATGCTACTTTTGAACTGAAATCTTGAAGTTTCTAATTTGCATTGTCATGGTGGAGGTGGTAAGGGAAAGCTTCTAGGCAGATTATTTCTAAACTTCTAATATTACTCTCGAGACGT

mRNA sequence

TAAATCCGATCCATATTCTTAAGGTAAGTTCTCAGTTTCGCATCGCTTAGCTAAATCATCTGCATCAAAACTAGCTTAAACCAGTGACCCGGATGGTCCGAGAATCTTTGCTGAATTTGTGGATTCTGGAGCATTTTCTTGCTTACGAAACAAAATTATTATTTCTTAGTTTCAATTTCGGAATGGTAATGCTTCGTTGCAGTACTATGCTTTTGCATTTATTGTGAATCTGATTTAGCAGTGAGCTATATAAAATGATGATCATTGTAGCCGCATTGGTTCTCGGTGTGTTGTTTGGCGCTCTGATTTTAATCCCACGCGACCGAAATGCACAAAAGGTTCAATTAAATAATATCAACAAGGCATCTAAAGTGTACAGTAAAGACGAGGTTTCGGTTCATAACAAGAGAACTGATTGCTGGATCATTATCAAGAACAAGGTGTACGATGTTACGTCCTATGTTGAAGAACATCCAGGAGGAGATGCTATCCTAGCACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACATGCAACTCGAGTCTTCGACATGATCGACGACTTCTATGTTGGAGATCTGAGGCTGTAATATCCAAGAAACATCTGGCTTTGCGCTATCACGTAGAGGACGTATTTCGCAGCGTTCCAGCTCTAGAGATGTCTTTCAGGGAATATATCAAATGATTTGAATTGTCTGGTTAGCATTCTTGCGATGTTGTAACATTTGTAGCTGTACACTTTCATTTAGATCTTCAACTTTCTAGTTTTATCTCTTTAGTTTGAATTCTTCTTTCTTTTTTTTATGATTGCAAGAGGAGAGAAACTTTTCATAGTGGTCTATTTTGATGCTACTTTTGAACTGAAATCTTGAAGTTTCTAATTTGCATTGTCATGGTGGAGGTGGTAAGGGAAAGCTTCTAGGCAGATTATTTCTAAACTTCTAATATTACTCTCGAGACGT

Coding sequence (CDS)

ATGATGATCATTGTAGCCGCATTGGTTCTCGGTGTGTTGTTTGGCGCTCTGATTTTAATCCCACGCGACCGAAATGCACAAAAGGTTCAATTAAATAATATCAACAAGGCATCTAAAGTGTACAGTAAAGACGAGGTTTCGGTTCATAACAAGAGAACTGATTGCTGGATCATTATCAAGAACAAGGTGTACGATGTTACGTCCTATGTTGAAGAACATCCAGGAGGAGATGCTATCCTAGCACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACATGCAACTCGAGTCTTCGACATGATCGACGACTTCTATGTTGGAGATCTGAGGCTGTAA

Protein sequence

MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL

Homology

[Close]

BLAST of Carg18596 vs. NCBI nr
Match: KAG7021304.1 (Cytochrome B5-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 228.4 bits (581), Expect = 3.1e-56
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60
           MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60

Query: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Carg18596 vs. NCBI nr
Match: KAG6586450.1 (Mitochondrial import inner membrane translocase subunit TIM17-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 228.4 bits (581), Expect = 3.1e-56
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60
           MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60

Query: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Carg18596 vs. NCBI nr
Match: XP_022937485.1 (cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_022937486.1 cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_022937487.1 cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_023536891.1 cytochrome B5-like protein [Cucurbita pepo subsp. pepo] >XP_023536892.1 cytochrome B5-like protein [Cucurbita pepo subsp. pepo] >XP_023536893.1 cytochrome B5-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.5 bits (576), Expect = 1.2e-55
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60
           MMIIVAALVLGVLFGALILIPRDRNAQKVQLNN NKASKVYSKDEVSVHNKRTDCWIIIK
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNTNKASKVYSKDEVSVHNKRTDCWIIIK 60

Query: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Carg18596 vs. NCBI nr
Match: XP_022966021.1 (cytochrome B5-like protein isoform X2 [Cucurbita maxima] >XP_022966023.1 cytochrome B5-like protein isoform X2 [Cucurbita maxima] >XP_022966024.1 cytochrome B5-like protein isoform X2 [Cucurbita maxima])

HSP 1 Score: 223.0 bits (567), Expect = 1.3e-54
Identity = 111/114 (97.37%), Postives = 112/114 (98.25%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60
           MMIIVAALVLGVLFGALILIPRDRNAQKVQLN+ NKASKVY KDEVSVHNKRTDCWIIIK
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKVYGKDEVSVHNKRTDCWIIIK 60

Query: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Carg18596 vs. NCBI nr
Match: XP_022937481.1 (cytochrome B5-like protein isoform X1 [Cucurbita moschata] >XP_022937482.1 cytochrome B5-like protein isoform X1 [Cucurbita moschata] >XP_022937484.1 cytochrome B5-like protein isoform X1 [Cucurbita moschata])

HSP 1 Score: 221.1 bits (562), Expect = 4.9e-54
Identity = 113/117 (96.58%), Postives = 113/117 (96.58%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDR---NAQKVQLNNINKASKVYSKDEVSVHNKRTDCWI 60
           MMIIVAALVLGVLFGALILIPRDR   NAQKVQLNN NKASKVYSKDEVSVHNKRTDCWI
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRKFGNAQKVQLNNTNKASKVYSKDEVSVHNKRTDCWI 60

Query: 61  IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 117

[Close]

BLAST of Carg18596 vs. ExPASy Swiss-Prot
Match: O22704 (Cytochrome B5-like protein OS=Arabidopsis thaliana OX=3702 GN=CB5LP PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 4.5e-34
Identity = 75/120 (62.50%), Postives = 85/120 (70.83%), Query Frame = 0


Query: 2   MIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKAS---------KVYSKDEVSVHNKR 61
           MI V  L+LG L  AL LI   R            +S         K YSK EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDL 113
            DCWIIIK+KVYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMI+DFY+G+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of Carg18596 vs. ExPASy Swiss-Prot
Match: Q9FDW8 (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-15
Identity = 34/76 (44.74%), Postives = 56/76 (73.68%), Query Frame = 0


Query: 38  SKVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTEGFYGPQH 97
           +K+YS +E + HNK+ DCW++I  KVYDV+SY++EHPGG D +LA AG D+T+ F    H
Sbjct: 5   TKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGH 64

Query: 98  ATRVFDMIDDFYVGDL 113
           +    ++++ +++G+L
Sbjct: 65  SKDARELMEKYFIGEL 80

[Close]

BLAST of Carg18596 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-15
Identity = 38/83 (45.78%), Postives = 59/83 (71.08%), Query Frame = 0


Query: 31  LNNINKASKVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTE 90
           ++N NK  KVY+ +EV+ HN + DCW+II  KVY+V+ ++E+HPGG D +L+  G D+T+
Sbjct: 1   MSNDNK--KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATD 60

Query: 91  GFYGPQHATRVFDMIDDFYVGDL 113
            F    H+T    M+D++YVGD+
Sbjct: 61  DFEDVGHSTTARAMMDEYYVGDI 81

[Close]

BLAST of Carg18596 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 4.7e-15
Identity = 35/76 (46.05%), Postives = 53/76 (69.74%), Query Frame = 0


Query: 38  SKVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAHA-GDDSTEGFYGPQH 97
           +KV++  EVS HN   DCW++I  KVYDVT ++++HPGGD +L  A G D+T+ F    H
Sbjct: 5   TKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFEDVGH 64

Query: 98  ATRVFDMIDDFYVGDL 113
           ++    M+D++YVGD+
Sbjct: 65  SSSARAMLDEYYVGDI 80

[Close]

BLAST of Carg18596 vs. ExPASy Swiss-Prot
Match: Q9ZNW2 (Acyl-lipid (9-3)-desaturase OS=Physcomitrium patens OX=3218 GN=DES6 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 6.1e-15
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 45  EVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMI 104
           EV+VHNK +DCWI++KNKVYDV+++ +EHPGG  I  + G D T+ F    HA   + ++
Sbjct: 109 EVAVHNKPSDCWIVVKNKVYDVSNFADEHPGGSVISTYFGRDGTDVF-SSFHAASTWKIL 168

Query: 105 DDFYVGDL 113
            DFY+GD+
Sbjct: 169 QDFYIGDV 175

[Close]

BLAST of Carg18596 vs. ExPASy TrEMBL
Match: A0A6J1FBC0 (cytochrome B5-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443883 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.7e-56
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60
           MMIIVAALVLGVLFGALILIPRDRNAQKVQLNN NKASKVYSKDEVSVHNKRTDCWIIIK
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNTNKASKVYSKDEVSVHNKRTDCWIIIK 60

Query: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Carg18596 vs. ExPASy TrEMBL
Match: A0A6J1HN83 (cytochrome B5-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465731 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.3e-55
Identity = 111/114 (97.37%), Postives = 112/114 (98.25%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVYSKDEVSVHNKRTDCWIIIK 60
           MMIIVAALVLGVLFGALILIPRDRNAQKVQLN+ NKASKVY KDEVSVHNKRTDCWIIIK
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKVYGKDEVSVHNKRTDCWIIIK 60

Query: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  NKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Carg18596 vs. ExPASy TrEMBL
Match: A0A6J1FAG5 (cytochrome B5-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443883 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.4e-54
Identity = 113/117 (96.58%), Postives = 113/117 (96.58%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDR---NAQKVQLNNINKASKVYSKDEVSVHNKRTDCWI 60
           MMIIVAALVLGVLFGALILIPRDR   NAQKVQLNN NKASKVYSKDEVSVHNKRTDCWI
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRKFGNAQKVQLNNTNKASKVYSKDEVSVHNKRTDCWI 60

Query: 61  IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 117

[Close]

BLAST of Carg18596 vs. ExPASy TrEMBL
Match: A0A6J1HQM7 (cytochrome B5-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465731 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.6e-53
Identity = 111/117 (94.87%), Postives = 112/117 (95.73%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDR---NAQKVQLNNINKASKVYSKDEVSVHNKRTDCWI 60
           MMIIVAALVLGVLFGALILIPRDR   NAQKVQLN+ NKASKVY KDEVSVHNKRTDCWI
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRKLGNAQKVQLNSTNKASKVYGKDEVSVHNKRTDCWI 60

Query: 61  IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL
Sbjct: 61  IIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 117

[Close]

BLAST of Carg18596 vs. ExPASy TrEMBL
Match: A0A1S3C893 (cytochrome B5-like protein OS=Cucumis melo OX=3656 GN=LOC103498106 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.8e-49
Identity = 100/115 (86.96%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKV-YSKDEVSVHNKRTDCWIII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN INK+SKV YSKDEVSVHNKRTDCW+II
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 115
           KNKVYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of Carg18596 vs. TAIR 10
Match: AT1G60660.1 (cytochrome B5-like protein )

HSP 1 Score: 145.2 bits (365), Expect = 3.2e-35
Identity = 75/120 (62.50%), Postives = 85/120 (70.83%), Query Frame = 0


Query: 2   MIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKAS---------KVYSKDEVSVHNKR 61
           MI V  L+LG L  AL LI   R            +S         K YSK EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDL 113
            DCWIIIK+KVYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMI+DFY+G+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of Carg18596 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 84.0 bits (206), Expect = 8.8e-17
Identity = 34/76 (44.74%), Postives = 56/76 (73.68%), Query Frame = 0


Query: 38  SKVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTEGFYGPQH 97
           +K+YS +E + HNK+ DCW++I  KVYDV+SY++EHPGG D +LA AG D+T+ F    H
Sbjct: 5   TKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGH 64

Query: 98  ATRVFDMIDDFYVGDL 113
           +    ++++ +++G+L
Sbjct: 65  SKDARELMEKYFIGEL 80

[Close]

BLAST of Carg18596 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 80.5 bits (197), Expect = 9.7e-16
Identity = 35/75 (46.67%), Postives = 51/75 (68.00%), Query Frame = 0


Query: 39  KVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGD-AILAHAGDDSTEGFYGPQHA 98
           KV++  EVS H+   DCWI+I  KVYDVT ++++HPGGD  IL   G D+T+ F    H+
Sbjct: 6   KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHS 65

Query: 99  TRVFDMIDDFYVGDL 113
           +    M+D++YVGD+
Sbjct: 66  STAKAMLDEYYVGDI 80

[Close]

BLAST of Carg18596 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 80.1 bits (196), Expect = 1.3e-15
Identity = 35/75 (46.67%), Postives = 51/75 (68.00%), Query Frame = 0


Query: 39  KVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGD-AILAHAGDDSTEGFYGPQHA 98
           KV S +EVS HNK  DCW+II  KVYDVT ++++HPGGD  +L+  G D+T  F    H+
Sbjct: 6   KVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHS 65

Query: 99  TRVFDMIDDFYVGDL 113
               DM+D +++G++
Sbjct: 66  DTARDMMDKYFIGEI 80

[Close]

BLAST of Carg18596 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 78.2 bits (191), Expect = 4.8e-15
Identity = 32/76 (42.11%), Postives = 53/76 (69.74%), Query Frame = 0


Query: 38  SKVYSKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTEGFYGPQH 97
           +K+++  EVS HN+  DCWI+I  KVY+VT ++E+HPGG D +L+  G D+T+ F    H
Sbjct: 5   AKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 64

Query: 98  ATRVFDMIDDFYVGDL 113
           +    +M++ +YVG++
Sbjct: 65  SESAREMMEQYYVGEI 80

The following BLAST results are available for this feature:

BLAST of Carg18596 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021304.1	3.1e-56	100.00	Cytochrome B5-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6586450.1	3.1e-56	100.00	Mitochondrial import inner membrane translocase subunit TIM17-2, partial [Cucurb...	[more]
XP_022937485.1	1.2e-55	99.12	cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_022937486.1 cytoc...	[more]
XP_022966021.1	1.3e-54	97.37	cytochrome B5-like protein isoform X2 [Cucurbita maxima] >XP_022966023.1 cytochr...	[more]
XP_022937481.1	4.9e-54	96.58	cytochrome B5-like protein isoform X1 [Cucurbita moschata] >XP_022937482.1 cytoc...	[more]

BLAST of Carg18596 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22704	4.5e-34	62.50	Cytochrome B5-like protein OS=Arabidopsis thaliana OX=3702 GN=CB5LP PE=2 SV=1	[more]
Q9FDW8	1.2e-15	44.74	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
P49100	1.6e-15	45.78	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
P49098	4.7e-15	46.05	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9ZNW2	6.1e-15	50.00	Acyl-lipid (9-3)-desaturase OS=Physcomitrium patens OX=3218 GN=DES6 PE=1 SV=1	[more]

BLAST of Carg18596 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FBC0	5.7e-56	99.12	cytochrome B5-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]
A0A6J1HN83	6.3e-55	97.37	cytochrome B5-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146573...	[more]
A0A6J1FAG5	2.4e-54	96.58	cytochrome B5-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]
A0A6J1HQM7	2.6e-53	94.87	cytochrome B5-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146573...	[more]
A0A1S3C893	1.8e-49	86.96	cytochrome B5-like protein OS=Cucumis melo OX=3656 GN=LOC103498106 PE=3 SV=1	[more]

BLAST of Carg18596 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60660.1	3.2e-35	62.50	cytochrome B5-like protein	[more]
AT1G26340.1	8.8e-17	44.74	cytochrome B5 isoform A	[more]
AT5G48810.1	9.7e-16	46.67	cytochrome B5 isoform D	[more]
AT5G53560.1	1.3e-15	46.67	cytochrome B5 isoform E	[more]
AT2G32720.1	4.8e-15	42.11	cytochrome B5 isoform B	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 73..87 score: 31.82 coord: 100..112 score: 27.16 coord: 63..73 score: 49.45
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 41..113 e-value: 3.5E-24 score: 96.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 43..112 e-value: 7.5E-21 score: 74.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 38..113 score: 25.271698
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 31..114 e-value: 4.7E-29 score: 102.2
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 36..112
None	No IPR available	PANTHER	PTHR19359:SF95	CYTOCHROME B5-LIKE PROTEIN	coord: 2..113
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 2..113
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 69..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18596-RA	Carg18596-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding