Carg18574 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg18574 vs. NCBI nr
Match: KAG7026960.1 (Indole-3-acetic acid-amido synthetase GH3.4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491.5 bits (1264), Expect = 4.1e-135
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTLPPRPNPELPNSFRNEVPRRTG 60
           MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTLPPRPNPELPNSFRNEVPRRTG
Sbjct: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTLPPRPNPELPNSFRNEVPRRTG 60

Query: 61  RDRHKGFGPTLNTCRDVERSHGSDIHANPTEIAYTILPNMAYFEFLPYDTSFSPNLSPPR 120
           RDRHKGFGPTLNTCRDVERSHGSDIHANPTEIAYTILPNMAYFEFLPYDTSFSPNLSPPR
Sbjct: 61  RDRHKGFGPTLNTCRDVERSHGSDIHANPTEIAYTILPNMAYFEFLPYDTSFSPNLSPPR 120

Query: 121 LVDLADVEIGKSHGFHNMAPQFRFVKRKNAMLCIDVDNTDEAELQQAVENASVLFAETLP 180
           LVDLADVEIGKSHGFHNMAPQFRFVKRKNAMLCIDVDNTDEAELQQAVENASVLFAETLP
Sbjct: 121 LVDLADVEIGKSHGFHNMAPQFRFVKRKNAMLCIDVDNTDEAELQQAVENASVLFAETLP 180

Query: 181 NSPPEEILNQCCLTIEESLNSVYIQSRVSFNSVGPLEIRGNFSALQPVKAALDTGVTPF 240
           NSPPEEILNQCCLTIEESLNSVYIQSRVSFNSVGPLEIRGNFSALQPVKAALDTGVTPF
Sbjct: 181 NSPPEEILNQCCLTIEESLNSVYIQSRVSFNSVGPLEIRGNFSALQPVKAALDTGVTPF 239

BLAST of Carg18574 vs. NCBI nr
Match: KAG6595005.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 250.0 bits (637), Expect = 2.1e-62
Identity = 171/342 (50.00%), Postives = 186/342 (54.39%), Query Frame = 0

Query: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS----------------------- 60
           MEAILCADS  + ++ QMLCGLLMRRQVLRVGAIFAS                       
Sbjct: 196 MEAILCADSF-QSMYTQMLCGLLMRRQVLRVGAIFASTLLRVIRFLQLNWKQLSHDISTG 255

Query: 61  TLPPR-----------------PNPELPNSFRNE-----------------------VPR 120
           TL P+                 PNPEL     NE                       V  
Sbjct: 256 TLNPKITDSSLRECIISKYLTGPNPELAQFVSNECSTEDWEGIVTRIWPNTKYLDVIVSG 315

Query: 121 RTGR-----DRHKGFGPTLNTC-RDVERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYD 180
             G+     + +    P ++T     E S G ++     PTEIAYTILPNMAYFEFLPYD
Sbjct: 316 AMGQYIPMLEFYSRSLPMVSTAYASSECSFGVNLSPMCKPTEIAYTILPNMAYFEFLPYD 375

Query: 181 TSFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKR 220
           TSFSP LSPPRLVDLADVEIGK +                      GFHNMAPQFRFVKR
Sbjct: 376 TSFSPILSPPRLVDLADVEIGKKYELVITTYAGLYRYRVGDVLQVTGFHNMAPQFRFVKR 435

BLAST of Carg18574 vs. NCBI nr
Match: KAG7027034.1 (putative indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250.0 bits (637), Expect = 2.1e-62
Identity = 171/342 (50.00%), Postives = 186/342 (54.39%), Query Frame = 0

Query: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS----------------------- 60
           MEAILCADS  + ++ QMLCGLLMRRQVLRVGAIFAS                       
Sbjct: 196 MEAILCADSF-QSMYTQMLCGLLMRRQVLRVGAIFASTLLRVIRFLQLNWKQLSHDISTG 255

Query: 61  TLPPR-----------------PNPELPNSFRNE-----------------------VPR 120
           TL P+                 PNPEL     NE                       V  
Sbjct: 256 TLNPKITDSSLRECIISKYLTGPNPELAQFVSNECSTEDWEGIVTRIWPNTKYLDVIVSG 315

Query: 121 RTGR-----DRHKGFGPTLNTC-RDVERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYD 180
             G+     + +    P ++T     E S G ++     PTEIAYTILPNMAYFEFLPYD
Sbjct: 316 AMGQYIPMLEFYSRSLPMVSTAYASSECSFGVNLSPMCKPTEIAYTILPNMAYFEFLPYD 375

Query: 181 TSFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKR 220
           TSFSP LSPPRLVDLADVEIGK +                      GFHNMAPQFRFVKR
Sbjct: 376 TSFSPILSPPRLVDLADVEIGKKYELVITTYAGLYRYRVGDVLQVTGFHNMAPQFRFVKR 435

BLAST of Carg18574 vs. NCBI nr
Match: XP_022962655.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 248.8 bits (634), Expect = 4.6e-62
Identity = 170/342 (49.71%), Postives = 186/342 (54.39%), Query Frame = 0

Query: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS----------------------- 60
           MEAILCADS  + ++ QMLCGLLMRRQVLRVGAIFAS                       
Sbjct: 196 MEAILCADSF-QSMYTQMLCGLLMRRQVLRVGAIFASTLLRVIRFLQLNWKQLSHDISTG 255

Query: 61  TLPPR-----------------PNPELPNSFRNE-----------------------VPR 120
           TL P+                 PNPEL     NE                       V  
Sbjct: 256 TLNPKITDSSLRECIISKYLTGPNPELAQFVSNECSTEDWEGIVTRIWPNTKYLDVIVSG 315

Query: 121 RTGR-----DRHKGFGPTLNTC-RDVERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYD 180
             G+     + +    P ++T     E S G ++     PTEIAYTILPNMAYFEFLPYD
Sbjct: 316 AMGQYIPMLEFYSRSLPMVSTAYASSECSFGVNLSPMCKPTEIAYTILPNMAYFEFLPYD 375

Query: 181 TSFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKR 220
           TSFSP LSPPRLVDLADV+IGK +                      GFHNMAPQFRFVKR
Sbjct: 376 TSFSPILSPPRLVDLADVDIGKKYELVITTYAGLYRYRVGDVLQVTGFHNMAPQFRFVKR 435

BLAST of Carg18574 vs. NCBI nr
Match: XP_023003445.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima])

HSP 1 Score: 234.6 bits (597), Expect = 9.1e-58
Identity = 166/345 (48.12%), Postives = 183/345 (53.04%), Query Frame = 0

Query: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS----------------------- 60
           +EAILCADS  + ++ QMLCGLLMRRQVLRVGAIFAS                       
Sbjct: 196 IEAILCADSF-QSMYTQMLCGLLMRRQVLRVGAIFASTLLRVIRFLQLNWKQLSHDISTG 255

Query: 61  TLPPR-----------------PNPELPNSFRNE-----------------------VPR 120
           TL P+                 PN EL      E                       V  
Sbjct: 256 TLNPKITDSSLRECIITKYLTGPNTELAQFVSKECSTEDWEGIVTRIWPNTKYLDVIVSG 315

Query: 121 RTGR-----DRHKGFGPTLNTC-RDVERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYD 180
             G+     + + G  P ++T     E S G ++     PTEIAYTILPNMAYFEFLPYD
Sbjct: 316 AMGQYIPMLEFYSGSLPMVSTAYASSECSFGVNLSPMCKPTEIAYTILPNMAYFEFLPYD 375

Query: 181 TSFSPNL---SPPRLVDLADVEIGKSH----------------------GFHNMAPQFRF 220
           TS SP L   SPPRLVDLADVEIGK +                      GFHNMAPQF F
Sbjct: 376 TSSSPILSPNSPPRLVDLADVEIGKKYELVITTYAGLYRYRVGDVLQVTGFHNMAPQFLF 435

BLAST of Carg18574 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.3e-34
Identity = 120/341 (35.19%), Postives = 157/341 (46.04%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS-----------------------T 61
           EAILC DS  + ++ QMLCGLLMR +VLR+GA+FAS                       T
Sbjct: 198 EAILCPDS-SQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGT 257

Query: 62  LPPR----------------PNPEL----------PNSFRNEVPR-----------RTGR 121
           L  R                P+ EL           NS+   + +            TG 
Sbjct: 258 LSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGA 317

Query: 122 --------DRHKGFGPTLNTCRDVERSH-GSDIH--ANPTEIAYTILPNMAYFEFLPYDT 181
                   + + G  P   T      S+ G ++     P+E++YTI+PNMAYFEFLP+  
Sbjct: 318 MAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPH-- 377

Query: 182 SFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRK 220
                     LV+LADVE+GK +                      GF+N APQF+FV+RK
Sbjct: 378 -HEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRK 437

BLAST of Carg18574 vs. ExPASy Swiss-Prot
Match: Q9LQ68 (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=GH3.4 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.6e-33
Identity = 118/366 (32.24%), Postives = 160/366 (43.72%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTLPPRPNPELPNSFRN-EVPRRTG 61
           EAILC DS  + ++ QMLCGLLMR +V R+GA+F S L  R    L N+++       TG
Sbjct: 197 EAILCCDS-SQSMYTQMLCGLLMRHEVNRLGAVFPSGL-LRAISFLQNNWKELSQDISTG 256

Query: 62  RDRHKGFGPTLNT--------------------CR------------------DV----- 121
               K F   + T                    C                   DV     
Sbjct: 257 TLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGA 316

Query: 122 -------------------------ERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYDT 181
                                    E   G +++    P+E++YTI PNMAYFEFLP++ 
Sbjct: 317 MAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNH 376

Query: 182 SFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRK 240
                +    LV+LADVE+GK +                      GFHN APQF+F++R+
Sbjct: 377 DGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRE 436

BLAST of Carg18574 vs. ExPASy Swiss-Prot
Match: Q9SZT9 (Indole-3-acetic acid-amido synthetase GH3.2 OS=Arabidopsis thaliana OX=3702 GN=GH3.2 PE=1 SV=3)

HSP 1 Score: 143.3 bits (360), Expect = 3.6e-33
Identity = 118/344 (34.30%), Postives = 158/344 (45.93%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTLPPRPNPELPNSFRNEVPR--RT 61
           EAILC+DS  + ++ QMLCGLLMR +VLR+GA+FAS L  R    L N+++ E+ R   T
Sbjct: 199 EAILCSDS-SQSMYAQMLCGLLMRHEVLRLGAVFASGL-LRAISFLQNNWK-ELARDIST 258

Query: 62  GRDRHKGFGPTL--------------------NTCR------------------DV---- 121
           G    + F P +                      C                   DV    
Sbjct: 259 GTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTG 318

Query: 122 --------------------------ERSHGSDIH--ANPTEIAYTILPNMAYFEFLPY- 181
                                     E   G ++     P+E++YTI+PNMAYFEFLP+ 
Sbjct: 319 AMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHN 378

Query: 182 ---DTSFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFR 217
              D +   +L    LV+LA+VE+GK +                      GFHN APQF+
Sbjct: 379 HDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFK 438

BLAST of Carg18574 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.8e-32
Identity = 119/340 (35.00%), Postives = 150/340 (44.12%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTL---------------------- 61
           EAILC DS  + ++ QMLCGLL R  VLRVGA+FAS L                      
Sbjct: 198 EAILCPDSF-QSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 257

Query: 62  -------PP---------RPNPELPNSFRNE---------VPRRTGRDRH---------K 121
                  P          +P+P L    R E         + R     ++          
Sbjct: 258 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 317

Query: 122 GFGPTLN--------TCRDVERSH---GSDIH--ANPTEIAYTILPNMAYFEFLPYDTSF 181
            + PTL          C     S    G +++  + P+E++YTI+PNMAYFEF+P     
Sbjct: 318 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIP----- 377

Query: 182 SPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRKNA 220
              L   + V+L DV IGK +                      GFHN APQF FV+RKN 
Sbjct: 378 ---LGGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 437

BLAST of Carg18574 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 136.0 bits (341), Expect = 5.7e-31
Identity = 112/339 (33.04%), Postives = 150/339 (44.25%), Query Frame = 0

Query: 3   AILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS-----------------------TL 62
           AILCAD+  + ++ QM+CGL  R  VLR+GA+FAS                        L
Sbjct: 206 AILCADAF-QSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGEL 265

Query: 63  PPR---------------PNPELPNSFRNEVPRR--------------------TGR--- 122
            PR               P+PEL    R E  +                     TG    
Sbjct: 266 TPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQ 325

Query: 123 -----DRHKGFGPTLNTC-RDVERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYDTSFS 182
                + + G  P   T     E   G ++    +P+E++YTI+PNM YFEFLP D + +
Sbjct: 326 YIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGA 385

Query: 183 PNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRKNAM 220
            +    +LVDLA VE+G+ +                      GFHN APQFRFV+RKN +
Sbjct: 386 ASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVL 445

BLAST of Carg18574 vs. ExPASy TrEMBL
Match: A0A6J1HFQ1 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111463077 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.2e-62
Identity = 170/342 (49.71%), Postives = 186/342 (54.39%), Query Frame = 0

Query: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS----------------------- 60
           MEAILCADS  + ++ QMLCGLLMRRQVLRVGAIFAS                       
Sbjct: 196 MEAILCADSF-QSMYTQMLCGLLMRRQVLRVGAIFASTLLRVIRFLQLNWKQLSHDISTG 255

Query: 61  TLPPR-----------------PNPELPNSFRNE-----------------------VPR 120
           TL P+                 PNPEL     NE                       V  
Sbjct: 256 TLNPKITDSSLRECIISKYLTGPNPELAQFVSNECSTEDWEGIVTRIWPNTKYLDVIVSG 315

Query: 121 RTGR-----DRHKGFGPTLNTC-RDVERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYD 180
             G+     + +    P ++T     E S G ++     PTEIAYTILPNMAYFEFLPYD
Sbjct: 316 AMGQYIPMLEFYSRSLPMVSTAYASSECSFGVNLSPMCKPTEIAYTILPNMAYFEFLPYD 375

Query: 181 TSFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKR 220
           TSFSP LSPPRLVDLADV+IGK +                      GFHNMAPQFRFVKR
Sbjct: 376 TSFSPILSPPRLVDLADVDIGKKYELVITTYAGLYRYRVGDVLQVTGFHNMAPQFRFVKR 435

BLAST of Carg18574 vs. ExPASy TrEMBL
Match: A0A6J1KRS6 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111497055 PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 4.4e-58
Identity = 166/345 (48.12%), Postives = 183/345 (53.04%), Query Frame = 0

Query: 1   MEAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS----------------------- 60
           +EAILCADS  + ++ QMLCGLLMRRQVLRVGAIFAS                       
Sbjct: 196 IEAILCADSF-QSMYTQMLCGLLMRRQVLRVGAIFASTLLRVIRFLQLNWKQLSHDISTG 255

Query: 61  TLPPR-----------------PNPELPNSFRNE-----------------------VPR 120
           TL P+                 PN EL      E                       V  
Sbjct: 256 TLNPKITDSSLRECIITKYLTGPNTELAQFVSKECSTEDWEGIVTRIWPNTKYLDVIVSG 315

Query: 121 RTGR-----DRHKGFGPTLNTC-RDVERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYD 180
             G+     + + G  P ++T     E S G ++     PTEIAYTILPNMAYFEFLPYD
Sbjct: 316 AMGQYIPMLEFYSGSLPMVSTAYASSECSFGVNLSPMCKPTEIAYTILPNMAYFEFLPYD 375

Query: 181 TSFSPNL---SPPRLVDLADVEIGKSH----------------------GFHNMAPQFRF 220
           TS SP L   SPPRLVDLADVEIGK +                      GFHNMAPQF F
Sbjct: 376 TSSSPILSPNSPPRLVDLADVEIGKKYELVITTYAGLYRYRVGDVLQVTGFHNMAPQFLF 435

BLAST of Carg18574 vs. ExPASy TrEMBL
Match: A0A6J1IRM5 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111478730 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 6.8e-43
Identity = 138/346 (39.88%), Postives = 164/346 (47.40%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS-----------------------T 61
           EA+LCADS  + ++ QMLCGLLMR QVLRVGA+FAS                       T
Sbjct: 198 EAVLCADSF-QSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGT 257

Query: 62  LPPR-----------------PNPELPNSFRNE---------VPRRTGRDRH-------- 121
           L P+                 PNPE+      E         + R     ++        
Sbjct: 258 LNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGA 317

Query: 122 -KGFGPTLN--------TCRDVERSH---GSDIH--ANPTEIAYTILPNMAYFEFLPYDT 181
              + PTL          C     S    G ++     P+E+ YTI+PNM YFEFLP+ T
Sbjct: 318 MAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHHT 377

Query: 182 SFSPNL---SPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFV 220
           S  P L   SPPRLVDLADVE+GK +                      GFHN APQFRFV
Sbjct: 378 SSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLEVTGFHNAAPQFRFV 437

BLAST of Carg18574 vs. ExPASy TrEMBL
Match: M5W865 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G226100 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 8.9e-43
Identity = 133/342 (38.89%), Postives = 169/342 (49.42%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS-----------------------T 61
           EAILCADS  + ++ QM+CGL+MR QVLRVGA+FAS                       T
Sbjct: 200 EAILCADSF-QSMYTQMVCGLVMRHQVLRVGAVFASGLLRAIRFLQLNWRQLAHDIHNGT 259

Query: 62  LPP---------------RPNPELPNSFRNE---------VPRRTGRDRH---------K 121
           L P               +P+PEL +    E         +PR     ++          
Sbjct: 260 LNPKITDPSLRESMSGTLKPDPELSSFITKECCEENWEGIIPRIWPNTKYLDVIVTGAMA 319

Query: 122 GFGPTLN--------TCRDVERSH---GSDIH--ANPTEIAYTILPNMAYFEFLPYDTSF 181
            + PTL+         C     S    G ++     P+E++YTI+PNMAYFEF+P+D S 
Sbjct: 320 QYIPTLDYYSNGLPLACTMYASSECYFGLNLKPMCKPSEVSYTIMPNMAYFEFIPHDPSA 379

Query: 182 SP--NLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRK 220
            P   LSPP LVDLADVE+GK +                      GFHN APQFRFV+RK
Sbjct: 380 PPLSKLSPPHLVDLADVEVGKEYELVITTYAGLCRYRVGDILEVTGFHNAAPQFRFVRRK 439

BLAST of Carg18574 vs. ExPASy TrEMBL
Match: A0A6J1GH54 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111453877 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 8.9e-43
Identity = 138/346 (39.88%), Postives = 164/346 (47.40%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS-----------------------T 61
           EA+LCADS  + ++ QMLCGLLMR QVLRVGA+FAS                       T
Sbjct: 198 EAVLCADSF-QSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGT 257

Query: 62  LPPR-----------------PNPELPNSFRNE---------VPRRTGRDRH-------- 121
           L P+                  NPE+      E         + R     ++        
Sbjct: 258 LNPKITDPCLRECIVSKYLTNTNPEVAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGA 317

Query: 122 -KGFGPTLN--------TCRDVERSH---GSDIH--ANPTEIAYTILPNMAYFEFLPYDT 181
              + PTL          C     S    G ++     P+E+ YTI+PNM YFEFLP+DT
Sbjct: 318 MAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDT 377

Query: 182 SFSPNL---SPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFV 220
           S  P L   SPPRLVDLADVE+GK +                      GFHN APQFRFV
Sbjct: 378 SSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLEVTGFHNAAPQFRFV 437

BLAST of Carg18574 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 148.7 bits (374), Expect = 6.1e-36
Identity = 121/347 (34.87%), Postives = 161/347 (46.40%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTLPPRPNPELPNSFRNEVPR--RT 61
           EAILC+DS  + ++ QMLCGLLMR +VLR+GA+FAS L  R    L N+++ E+ R   T
Sbjct: 199 EAILCSDS-SQSMYAQMLCGLLMRHEVLRLGAVFASGL-LRAISFLQNNWK-ELARDIST 258

Query: 62  GRDRHKGFGPTL--------------------NTCR------------------DV---- 121
           G    + F P +                      C                   DV    
Sbjct: 259 GTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTG 318

Query: 122 --------------------------ERSHGSDIH--ANPTEIAYTILPNMAYFEFLPY- 181
                                     E   G ++     P+E++YTI+PNMAYFEFLP+ 
Sbjct: 319 AMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHN 378

Query: 182 ---DTSFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFR 220
              D +   +L    LV+LA+VE+GK +                      GFHN APQF+
Sbjct: 379 HDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFK 438

BLAST of Carg18574 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 146.7 bits (369), Expect = 2.3e-35
Identity = 120/341 (35.19%), Postives = 157/341 (46.04%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS-----------------------T 61
           EAILC DS  + ++ QMLCGLLMR +VLR+GA+FAS                       T
Sbjct: 198 EAILCPDS-SQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGT 257

Query: 62  LPPR----------------PNPEL----------PNSFRNEVPR-----------RTGR 121
           L  R                P+ EL           NS+   + +            TG 
Sbjct: 258 LSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGA 317

Query: 122 --------DRHKGFGPTLNTCRDVERSH-GSDIH--ANPTEIAYTILPNMAYFEFLPYDT 181
                   + + G  P   T      S+ G ++     P+E++YTI+PNMAYFEFLP+  
Sbjct: 318 MAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPH-- 377

Query: 182 SFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRK 220
                     LV+LADVE+GK +                      GF+N APQF+FV+RK
Sbjct: 378 -HEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRK 437

BLAST of Carg18574 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.1e-34
Identity = 118/366 (32.24%), Postives = 160/366 (43.72%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTLPPRPNPELPNSFRN-EVPRRTG 61
           EAILC DS  + ++ QMLCGLLMR +V R+GA+F S L  R    L N+++       TG
Sbjct: 197 EAILCCDS-SQSMYTQMLCGLLMRHEVNRLGAVFPSGL-LRAISFLQNNWKELSQDISTG 256

Query: 62  RDRHKGFGPTLNT--------------------CR------------------DV----- 121
               K F   + T                    C                   DV     
Sbjct: 257 TLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGA 316

Query: 122 -------------------------ERSHGSDIH--ANPTEIAYTILPNMAYFEFLPYDT 181
                                    E   G +++    P+E++YTI PNMAYFEFLP++ 
Sbjct: 317 MAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNH 376

Query: 182 SFSPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRK 240
                +    LV+LADVE+GK +                      GFHN APQF+F++R+
Sbjct: 377 DGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRE 436

BLAST of Carg18574 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.3e-33
Identity = 119/340 (35.00%), Postives = 150/340 (44.12%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFASTL---------------------- 61
           EAILC DS  + ++ QMLCGLL R  VLRVGA+FAS L                      
Sbjct: 198 EAILCPDSF-QSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 257

Query: 62  -------PP---------RPNPELPNSFRNE---------VPRRTGRDRH---------K 121
                  P          +P+P L    R E         + R     ++          
Sbjct: 258 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 317

Query: 122 GFGPTLN--------TCRDVERSH---GSDIH--ANPTEIAYTILPNMAYFEFLPYDTSF 181
            + PTL          C     S    G +++  + P+E++YTI+PNMAYFEF+P     
Sbjct: 318 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIP----- 377

Query: 182 SPNLSPPRLVDLADVEIGKSH----------------------GFHNMAPQFRFVKRKNA 220
              L   + V+L DV IGK +                      GFHN APQF FV+RKN 
Sbjct: 378 ---LGGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 437

BLAST of Carg18574 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.9e-29
Identity = 114/373 (30.56%), Postives = 159/373 (42.63%), Query Frame = 0

Query: 2   EAILCADSLPKHVHPQMLCGLLMRRQVLRVGAIFAS-----------------------T 61
           + ILC+DS  + ++ QMLCGL   ++VLRVGA+FAS                       T
Sbjct: 200 QTILCSDSY-QSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 259

Query: 62  LPP---------------RPNPELPNSFRNEVPRRTGR------------------DRHK 121
           L                 +P+P+L +   +E  + + +                      
Sbjct: 260 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 319

Query: 122 GFGPTLN--------TCRDVERSH---GSDIH--ANPTEIAYTILPNMAYFEFLPY--DT 181
            + PTL+         C     S    G ++     P+E++YT++PNMAYFEFLP   ++
Sbjct: 320 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 379

Query: 182 SFSPNLSPPR---------LVDLADVEIGKSH----------------------GFHNMA 240
             + ++S P+         LVDL DV++G+ +                      GF N A
Sbjct: 380 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 439