Carg18517 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18517
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cysteine desulfurase
Location	Carg_Chr07: 2744610 .. 2749216 (+)
RNA-Seq Expression	Carg18517
Synteny	Carg18517

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTAAGATTCTTGCATCCGGTTTACGCCGCACCCTCACGGAATTTTCTCCCGCCGTCTTGTGCTTACGCCATCTCTCCACTGCCGCTGCTGTAGCCACTTCTCCGTCGGAACTCCATGAGGATGAATCTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGCTTTATCTTGATATGCAAGCCACATCCCCTGTTGATCCTAGGGTGCTCGACGCTATGCTTCCTTACTATCTCTCTCGCTACGGAAATCCACACTCTCGGACGCATCTCTACGGCTGGGAGTCGGACCATGCCGTTGAGACGGCTCGCTCCCAAGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTTTTTACCTCTGGAGCTACTGAGTCCAATAACATATCCGTCAAAGGGGTGATGCATTTCTATAAAGAAAAGAAGAAGCATGTGATTACCACTCAGACCGAGCACAAGTGCGTTCTTGATTCGTGCAGACATTTGCAGCAGGAGGGTTTTGATATTACCTATTTACCGGTTGGGACCGATGGGATTGTTGATTTGGAGAAGCTTCGTTCCGCGATTAGACCTGATACTGGCCTTGTTTCGATCATGGCGGTTAATAATGAAATTGGTGTCATTCAGCCAGTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCTTTCCATACTGATGCGGCTCAGGCGCTTGGGAAGATTCCAATTGATGTGGAGAAATGGAATGTAAGCCTGATGTCTTTGAGTGGACATAAGGTTTATGGGCCAAAGGGTATTGGAGCTTTGTATTTGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAAATGAACGGAGGAGGACAAGAGAGGGGCATTAGGAGTGGGACCGTCCCAACCCCATTGGTCGTGGGAATGGGGGCAGCCTGTGAGCTTGCTATGAAGGAAATGGAATATGATGATAAGCGAATCACTGCGCTGCAGGAACGGCTCTTGAATGGCATAAATGCGAAACTTGAAGGAGTTGTGGTAAATGGTAGCATGGAGAAACGTTATCCTGGTAATCTGAACTTGTCATTTGCGTATGTGGAAGGAGAAAGCTTGCTTATGGGTTTGAAGGATGTGGCTGTTTCGAGCGGGAGTGCATGCACGAGCGCCAGCTTGGAGCCTTCATATGTGTTGAGGGCGTTGGGAGTGGATGAGGACATGGCTCACACTTCGATCAGATTTGGCATTGGGAGGTTCACCACGGAGGCTGAGATTGATCGAGCAGTTGAGTTGACTGTGCAGCAGGTGGAAAAGTTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAATATCAAGGAAATCCAGTGGGCTCAACACTGATTGCTTTTATTCTCTAGTATAAGATATCCATATGCCGCATGTCTGTTTGGAGTTGTATGGGATACAATGGATTGTTTGGCAGTAATGGCGAGGCATCTGCGCTATATTACGTTGTACCATATGGATACCCTTCCATGTTTTCTCCAGGTTTTCCTCATCCTCGCCCTTTTAGTGATCCGATGCTCTCAAGAAGCCCCTATATGCTCACGGAAGTTTTTTTTTTTTTAAATAATAATACGCAACCATGTCAGCATTTCCACTATTGCTTAATATGCAGATACTTCCCCTAGCCCCTACCCCCACCCCCCACAGCAGGATTGTGATTAGAACAAACTCGTAATCTAATGCATACTTCAATGGTTTTGGTTTTTCTGCCCCAATTGCTCAAGTAACTGTGGAAATGCTTTCATTAGCTTCTCCTGTTGTATGTTGTAGAATTGTGTCTGCTTTGCTGAAATTCAAGAAATAGAGATGTTTTAAATATTGGGGAAAAAAGGCCGAATGATTCTATATTTTCCAGCAAAAAATGCTGCAGTGGGCTATTTGTGGTTGGGACAATATATTTGACGTAGACCCCATGCTACTTCCATGTTTGCATAATTTTGATCAAAATTAGTATTATTTCATTTGTGCAATGCTTTTTTATCTAGCCATCAGGCACTTTAGTCCACTGAAGTTTATCTGAGCAAGATATAAAAGCTGGATTACTTTGTAGGTTGATCTGCCTGAAATTAGTAGCTGGCATAAAATTGAGCTCATGTCATCACTTCTGTCTGATCCTTCTAATTTACTGTGTCAGTTGCCATCTCCTCGAATCATTATGCGTTTGATCTCTCTGCTGATAGGTTTGATATCATGTATACTCTAGAATTGTTGGCTAGGCCATGTTTTGTGGTTCTTCATTGTAGTTTCACCATAATTATAACCGACCCTCTTGTTACTTCTTTACGAAGGTTAATGATTTTATTAAGTTCTTGATTTTTGTCTCTTTATTTTTGGTCCACCTAATTCAGCAAAGGGGACAGTCTACCTTTTTGTTTGTTTTCATTTGATTTTCTTCCCCATTCTTTTTAGTTGAAGAGGGGGAATTTATGGTTTTTCCTCCTCCTTCACTGTGTATAGCAGCAAAGGACAGTCTACATTTTATTTTCAAGTTTTTTAGTTAAGGCATACAATTTTTTATTAAAGATGATAGTACCAAGGGTCAGATTAGACATCCTCTCGTCCCAGCTCACAAGATTACAGAAATTCCTTACCCCAGGAGACAAGAATAGTTGTGAAATCATTTAGACAAAGGAATTACCATCGGTTGGTCTAGATATCAAGATTTTAGTGTCTGCTGTTCAGATTATCATGAATGGTTGTGATGGTACCCGCCTTAAGAAATATGGACACCTATGATCCAATCTCTCTTTTATATTGAGGTTCCTCCTGGAGAATGCATGGCATAAATTTAGAGGCTCTTTGCTGGGTATGTTTATGTGAGCTCTTTTTGAACATCAACATGTGCAGAGAGCTTGTAGCTCCATGAATGTGAAACTGTAGCATCTTTTGAAGTTGCTCAAGAGAATTCGCAGAAAAAAAAAAATTGAGACCATTTCAAAGTGAAAAACTTATACACATTCTCGACCGGATATGTCTGGAATTTGAAGTTTTCCAACTATGAACAGCAACTTTATGCAAATAAAATGGCTAGTACAATTGAGTAGCCATCTACAGTGACACTCTGGAACAAGAAGACTCTGTCTAGATATTAATTTATTCTTCCCCTGCTAGATCAGGACGCTCCTTTTTCTTTATGCAAGGTTTTGCTTCCACAAGGTTTTTCTTTCATTCAAGTTCAATTGCTTGGAGGTGCAATGCCGACCTTCACTCTCATGGTTCTCTCTTAGGTATATGAGAGGGATGCTAATCTAGTAGACTGAATTAATGTTACTGACTATAGTTTTCTTGGCTGGCTTGAAATTTATGTGTGCCTCTATTGACTACAGGTTCTAAGATGAACTACAAAAAGTGAACATCCTTAATCCACATCTTCTGTTGTTCAAAATGAAGTCGTACTTTTTGTGTACAATCAACAAAGAGTTCCTAAGGAAAGCTCTTAATCAGTAGATTATGTTTTATTATTGTTGTTGCCTATTATTTAATGCAGGATTCTTGGTTGGCTTGAATCCTCTAGAACTAACATCCTAAGTCGATTTCTGCCTTCTGCTGTCGAAAATGAATATGGTTACGGTAGGTCGAAAGTTCAGATCTGCAGCAGATAAATAGCAGAAGTGATTGAATGGCCTTGTCTAAGAATTCCAAGCTATACTTGGTCAGTTGAGGCCGCGTAGGAGCCCAGGAGACAGTTTCGTCTTCTCTATTCTGATGGACTTTCACTGTCTGGTAGCTAGCATTTTACACAAATCAGGACAATAGATGAATGTTTTAGTGTTCCGCTAAGAGTTTGTTAATATTGGCAGTTGTCTTGGTTTCTGCGTTCTTTTTAATGTTCAAAAGTGAAGTTATCAACAAACCCTCTTTCTCCATGAGCAGTCTACAACTTACTCTTTGACCATCTTGGGCGACCACCTCCTGCAGAAGACCAAGTATCAAGGAGTTCACATGTCAAAGTCGGAAACATATCACGTAAATAAGTAAGATTACTTGTCCTTGTCTGTATGTACATTTCGACTGTAGCAAAAGAGAACTTTGGATGAGGTGAGTTGGAGAAGGCTTCATAGTGGATCATGGTTGCACGTGGAGACTGTCAGAGGAAGAATGCTTTTAGGTTTAATGAGAAGAAGTAATGGCCATAATTTGGCCGATGCTTAATGGGAAGCTGGAAGGAATGTTGCAAATCCATCCTGTTGGGTAGTAGTAATCAATATGGATAGGGAAGGAGGATTGAGCATCCACCCTTCGATGTTTATAAGCGAGGAATACATCTCTATTGGTTTGAGACCTTTTAAGAAAAATTAAAAGCAAAATCGTGAAAGTTTGTGCTCAAAGTGGACAACATCATTCCTGCGGGTGTGTGTGTTTGGTTTGGTTGAATGATGACTTCCTTTCCCTGAGCCATAATTGAGACTGCATTCTTTTTAAAACATGCAGGTATTGTGGAGCAACATCCATTAAATGTGTTTTGTCTTCAGCTAAGAAAGCAACCGCTCAAAAGGGAGAGGGGGTCCAGCAGAAGAAGCAGCCATGTGCAGTGCAGTGCGGTGGCCAAGTATTTTGGCAATCTGTAA

mRNA sequence

ATGGCTTCTAAGATTCTTGCATCCGGTTTACGCCGCACCCTCACGGAATTTTCTCCCGCCGTCTTGTGCTTACGCCATCTCTCCACTGCCGCTGCTGTAGCCACTTCTCCGTCGGAACTCCATGAGGATGAATCTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGCTTTATCTTGATATGCAAGCCACATCCCCTGTTGATCCTAGGGTGCTCGACGCTATGCTTCCTTACTATCTCTCTCGCTACGGAAATCCACACTCTCGGACGCATCTCTACGGCTGGGAGTCGGACCATGCCGTTGAGACGGCTCGCTCCCAAGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTTTTTACCTCTGGAGCTACTGAGTCCAATAACATATCCGTCAAAGGGGTGATGCATTTCTATAAAGAAAAGAAGAAGCATGTGATTACCACTCAGACCGAGCACAAGTGCGTTCTTGATTCGTGCAGACATTTGCAGCAGGAGGGTTTTGATATTACCTATTTACCGGTTGGGACCGATGGGATTGTTGATTTGGAGAAGCTTCGTTCCGCGATTAGACCTGATACTGGCCTTGTTTCGATCATGGCGGTTAATAATGAAATTGGTGTCATTCAGCCAGTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCTTTCCATACTGATGCGGCTCAGGCGCTTGGGAAGATTCCAATTGATGTGGAGAAATGGAATGTAAGCCTGATGTCTTTGAGTGGACATAAGGTTTATGGGCCAAAGGGTATTGGAGCTTTGTATTTGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAAATGAACGGAGGAGGACAAGAGAGGGGCATTAGGAGTGGGACCGTCCCAACCCCATTGGTCGTGGGAATGGGGGCAGCCTGTGAGCTTGCTATGAAGGAAATGGAATATGATGATAAGCGAATCACTGCGCTGCAGGAACGGCTCTTGAATGGCATAAATGCGAAACTTGAAGGAGTTGTGGTAAATGGTAGCATGGAGAAACGTTATCCTGGTAATCTGAACTTGTCATTTGCGTATGTGGAAGGAGAAAGCTTGCTTATGGGTTTGAAGGATGTGGCTGTTTCGAGCGGGAGTGCATGCACGAGCGCCAGCTTGGAGCCTTCATATGTGTTGAGGGCGTTGGGAGTGGATGAGGACATGGCTCACACTTCGATCAGATTTGGCATTGGGAGGTTCACCACGGAGGCTGAGATTGATCGAGCAGTTGAGTTGACTGTGCAGCAGGTGGAAAAGTTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAATATCAAGGAAATCCATAATGGCGAGGCATCTGCGCTATATTACGTTGTACCATATGGATACCCTTCCATGTTTTCTCCAGTTGCCATCTCCTCGAATCATTATGCGTTTGATCTCTCTGCTGATAGGTTTGATATCATGTATACTCTAGAATTGTTGGCTAGGCCATGTTTTGTGGTTCTTCATTGTAGTTTCACCATAATTATAACCGACCCTCTTGTTACTTCTTTACGAAGGATTCTTGGTTGGCTTGAATCCTCTAGAACTAACATCCTAAGTCGATTTCTGCCTTCTGCTGTCGAAAATGAATATGGTTACGGTATTGTGGAGCAACATCCATTAAATGTGTTTTGTCTTCAGCTAAGAAAGCAACCGCTCAAAAGGGAGAGGGGGTCCAGCAGAAGAAGCAGCCATGTGCAGTGCAGTGCGGTGGCCAAGTATTTTGGCAATCTGTAA

Coding sequence (CDS)

ATGGCTTCTAAGATTCTTGCATCCGGTTTACGCCGCACCCTCACGGAATTTTCTCCCGCCGTCTTGTGCTTACGCCATCTCTCCACTGCCGCTGCTGTAGCCACTTCTCCGTCGGAACTCCATGAGGATGAATCTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGCTTTATCTTGATATGCAAGCCACATCCCCTGTTGATCCTAGGGTGCTCGACGCTATGCTTCCTTACTATCTCTCTCGCTACGGAAATCCACACTCTCGGACGCATCTCTACGGCTGGGAGTCGGACCATGCCGTTGAGACGGCTCGCTCCCAAGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTTTTTACCTCTGGAGCTACTGAGTCCAATAACATATCCGTCAAAGGGGTGATGCATTTCTATAAAGAAAAGAAGAAGCATGTGATTACCACTCAGACCGAGCACAAGTGCGTTCTTGATTCGTGCAGACATTTGCAGCAGGAGGGTTTTGATATTACCTATTTACCGGTTGGGACCGATGGGATTGTTGATTTGGAGAAGCTTCGTTCCGCGATTAGACCTGATACTGGCCTTGTTTCGATCATGGCGGTTAATAATGAAATTGGTGTCATTCAGCCAGTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCTTTCCATACTGATGCGGCTCAGGCGCTTGGGAAGATTCCAATTGATGTGGAGAAATGGAATGTAAGCCTGATGTCTTTGAGTGGACATAAGGTTTATGGGCCAAAGGGTATTGGAGCTTTGTATTTGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAAATGAACGGAGGAGGACAAGAGAGGGGCATTAGGAGTGGGACCGTCCCAACCCCATTGGTCGTGGGAATGGGGGCAGCCTGTGAGCTTGCTATGAAGGAAATGGAATATGATGATAAGCGAATCACTGCGCTGCAGGAACGGCTCTTGAATGGCATAAATGCGAAACTTGAAGGAGTTGTGGTAAATGGTAGCATGGAGAAACGTTATCCTGGTAATCTGAACTTGTCATTTGCGTATGTGGAAGGAGAAAGCTTGCTTATGGGTTTGAAGGATGTGGCTGTTTCGAGCGGGAGTGCATGCACGAGCGCCAGCTTGGAGCCTTCATATGTGTTGAGGGCGTTGGGAGTGGATGAGGACATGGCTCACACTTCGATCAGATTTGGCATTGGGAGGTTCACCACGGAGGCTGAGATTGATCGAGCAGTTGAGTTGACTGTGCAGCAGGTGGAAAAGTTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAATATCAAGGAAATCCATAATGGCGAGGCATCTGCGCTATATTACGTTGTACCATATGGATACCCTTCCATGTTTTCTCCAGTTGCCATCTCCTCGAATCATTATGCGTTTGATCTCTCTGCTGATAGGTTTGATATCATGTATACTCTAGAATTGTTGGCTAGGCCATGTTTTGTGGTTCTTCATTGTAGTTTCACCATAATTATAACCGACCCTCTTGTTACTTCTTTACGAAGGATTCTTGGTTGGCTTGAATCCTCTAGAACTAACATCCTAAGTCGATTTCTGCCTTCTGCTGTCGAAAATGAATATGGTTACGGTATTGTGGAGCAACATCCATTAAATGTGTTTTGTCTTCAGCTAAGAAAGCAACCGCTCAAAAGGGAGAGGGGGTCCAGCAGAAGAAGCAGCCATGTGCAGTGCAGTGCGGTGGCCAAGTATTTTGGCAATCTGTAA

Protein sequence

MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKEIVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGTDGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEIHNGEASALYYVVPYGYPSMFSPVAISSNHYAFDLSADRFDIMYTLELLARPCFVVLHCSFTIIITDPLVTSLRRILGWLESSRTNILSRFLPSAVENEYGYGIVEQHPLNVFCLQLRKQPLKRERGSSRRSSHVQCSAVAKYFGNL

Homology

[Close]

BLAST of Carg18517 vs. NCBI nr
Match: KAG7026904.1 (Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 598/598 (100.00%), Postives = 598/598 (100.00%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIHNGEASALYYVVPYGYPSMFSPVAISSN 480
           RAVELTVQQVEKLREMSPLYEMVKEGINIKEIHNGEASALYYVVPYGYPSMFSPVAISSN
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIHNGEASALYYVVPYGYPSMFSPVAISSN 480

Query: 481 HYAFDLSADRFDIMYTLELLARPCFVVLHCSFTIIITDPLVTSLRRILGWLESSRTNILS 540
           HYAFDLSADRFDIMYTLELLARPCFVVLHCSFTIIITDPLVTSLRRILGWLESSRTNILS
Sbjct: 481 HYAFDLSADRFDIMYTLELLARPCFVVLHCSFTIIITDPLVTSLRRILGWLESSRTNILS 540

Query: 541 RFLPSAVENEYGYGIVEQHPLNVFCLQLRKQPLKRERGSSRRSSHVQCSAVAKYFGNL 599
           RFLPSAVENEYGYGIVEQHPLNVFCLQLRKQPLKRERGSSRRSSHVQCSAVAKYFGNL
Sbjct: 541 RFLPSAVENEYGYGIVEQHPLNVFCLQLRKQPLKRERGSSRRSSHVQCSAVAKYFGNL 598

[Close]

BLAST of Carg18517 vs. NCBI nr
Match: KAG6594943.1 (Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 587/610 (96.23%), Postives = 589/610 (96.56%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI-----------HNGEASALYYVVPYGYP 480
           RAVELTVQQVEKLREMSPLYEM K  I    +            NGEASALYYVVPYGYP
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMYKISICRMSVWSCMGYNGLFGSNGEASALYYVVPYGYP 480

Query: 481 SMFSPVAISSNHYAFDLSADRFDIMY-TLELLARPCFVVLHCSFTIIITDPLVTSLRRIL 540
           SMFSPVAIS NHYAFDLSADRFDIMY +LELLARPCFVVLHCSFTIIITDPLVTSLRRIL
Sbjct: 481 SMFSPVAISLNHYAFDLSADRFDIMYRSLELLARPCFVVLHCSFTIIITDPLVTSLRRIL 540

Query: 541 GWLESSRTNILSRFLPSAVENEYGYGIVEQHPLNVFCLQLRKQPLKRERGSSRRSSHVQC 599
           GWLESSRTNILSRFLPSAVENEYGYGIVEQHPLNVFCLQLRKQPLKRERGSSRRSSHVQC
Sbjct: 541 GWLESSRTNILSRFLPSAVENEYGYGIVEQHPLNVFCLQLRKQPLKRERGSSRRSSHVQC 600

[Close]

BLAST of Carg18517 vs. NCBI nr
Match: XP_023518255.1 (cysteine desulfurase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.2 bits (2286), Expect = 3.2e-253
Identity = 448/452 (99.12%), Postives = 451/452 (99.78%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILASGLRRTLTEFSPAVL LRH+STAAAVATSPSELHEDESGISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTEFSPAVLRLRHISTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNG+NAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGVNAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RAVELTVQQVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 452

[Close]

BLAST of Carg18517 vs. NCBI nr
Match: XP_023003261.1 (cysteine desulfurase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 881.7 bits (2277), Expect = 3.5e-252
Identity = 448/452 (99.12%), Postives = 449/452 (99.34%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILASGLRRTLT FSPAVL LRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTGFSPAVLRLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RAVELTV QVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEI 452

[Close]

BLAST of Carg18517 vs. NCBI nr
Match: XP_022963408.1 (cysteine desulfurase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 876.7 bits (2264), Expect = 1.1e-250
Identity = 449/452 (99.34%), Postives = 449/452 (99.34%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILASGLRRTLT FSPAVL LRHLSTAAAVATS SELHEDESGISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTGFSPAVLRLRHLSTAAAVATS-SELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RAVELTVQQVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 451

[Close]

BLAST of Carg18517 vs. ExPASy Swiss-Prot
Match: O49543 (Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=1 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 5.4e-203
Identity = 352/452 (77.88%), Postives = 401/452 (88.72%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASK++++ +RRTLT+        R+LSTAAA     +E++ ++  I MKGV+ISGRPLY
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQAT+P+DPRV DAM    +  YGNPHSRTHLYGWE+++AVE AR+QVA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVF SGATE+NN++VKGVMHFYK+ KKHVITTQTEHKCVLDSCRHLQQEGF++TYLPV T
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DG+VDLE LR AIRPDTGLVSIMAVNNEIGV+QP+EEIG ICKE NVPFHTDAAQA+GKI
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           P+DV+KWNV+LMS+S HK+YGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  
Sbjct: 241 PVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQ 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           +VG GAACELAMKEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAY
Sbjct: 301 IVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLK+VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID
Sbjct: 361 VEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           +AVELTV+QVEKLREMSPLYEMVKEGI+IK I
Sbjct: 421 KAVELTVKQVEKLREMSPLYEMVKEGIDIKNI 448

[Close]

BLAST of Carg18517 vs. ExPASy Swiss-Prot
Match: Q5RDE7 (Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 4.7e-167
Identity = 279/396 (70.45%), Postives = 337/396 (85.10%), Query Frame = 0


Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI+FTSGATESNNI++KGV  FY+ +KKH+ITTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGTDGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQA 236
           PV   GI+DL++L +AI+PDT LVS+M VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNL 356
           PTPLVVG+GAACE+A +EMEYD KRI+ L ERL+  I   L  VV+NG  E  YPG +NL
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPEHHYPGCINL 356

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFG+GRFTTE
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGVGRFTTE 416

Query: 417 AEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
            E+D  VE  +Q V++LREMSPL+EMV++GI++K I
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSI 452

[Close]

BLAST of Carg18517 vs. ExPASy Swiss-Prot
Match: Q9Y697 (Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3)

HSP 1 Score: 588.2 bits (1515), Expect = 1.1e-166
Identity = 279/396 (70.45%), Postives = 337/396 (85.10%), Query Frame = 0


Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI+FTSGATESNNI++KGV  FY+ +KKH+ITTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGTDGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQA 236
           PV   GI+DL++L +AI+PDT LVS+M VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNL 356
           PTPLVVG+GAACE+A +EMEYD KRI+ L ERL+  I   L  VV+NG  +  YPG +NL
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINL 356

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 416

Query: 417 AEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
            E+D  VE  +Q V++LREMSPL+EMV++GI++K I
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSI 452

[Close]

BLAST of Carg18517 vs. ExPASy Swiss-Prot
Match: Q9Z1J3 (Cysteine desulfurase, mitochondrial OS=Mus musculus OX=10090 GN=Nfs1 PE=1 SV=3)

HSP 1 Score: 583.2 bits (1502), Expect = 3.4e-165
Identity = 277/396 (69.95%), Postives = 336/396 (84.85%), Query Frame = 0


Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 59  RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 118

Query: 117 SPKEIVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI+FTSGATESNNI++KGV  FY+ +KKH++TTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 119 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 178

Query: 177 PVGTDGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQA 236
           PV   GI+DL++L +AI+PDT LVS+M VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 179 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKVYFHTDAAQA 238

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 239 VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 298

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNL 356
           PTPLVVG+GAACELA +EMEYD KRI+ L ERL+  I   L  VV+NG  ++ YPG +NL
Sbjct: 299 PTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNGDPKQHYPGCINL 358

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 359 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 418

Query: 417 AEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
            E+D   E  +  V++LREMSPL+EMV++GI++K I
Sbjct: 419 EEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSI 454

[Close]

BLAST of Carg18517 vs. ExPASy Swiss-Prot
Match: Q99P39 (Cysteine desulfurase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Nfs1 PE=2 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 2.2e-164
Identity = 275/396 (69.44%), Postives = 337/396 (85.10%), Query Frame = 0


Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D++AT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 51  RPLYMDVRATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 110

Query: 117 SPKEIVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI+FTSGATESNNI++KGV  FY+ +KKH++TTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 111 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 170

Query: 177 PVGTDGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQA 236
           PV   GI+DL++L +AI+PDT LVS+M VNNEIGV QP+ EIG+IC    + FHTDAAQA
Sbjct: 171 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQA 230

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 231 VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 290

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNL 356
           PTPLVVG+GAACELA +EMEYD KRI+ L ERL+  I   L  VV+NG  ++ YPG +NL
Sbjct: 291 PTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLPDVVMNGDPKQHYPGCINL 350

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 351 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 410

Query: 417 AEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
            E+D  V+  +  V++LREMSPL+EMV++GI++K I
Sbjct: 411 EEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSI 446

[Close]

BLAST of Carg18517 vs. ExPASy TrEMBL
Match: A0A6J1KNP3 (Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111496923 PE=3 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.7e-252
Identity = 448/452 (99.12%), Postives = 449/452 (99.34%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILASGLRRTLT FSPAVL LRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTGFSPAVLRLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RAVELTV QVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEI 452

[Close]

BLAST of Carg18517 vs. ExPASy TrEMBL
Match: A0A6J1HHQ0 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111463622 PE=3 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 5.5e-251
Identity = 449/452 (99.34%), Postives = 449/452 (99.34%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILASGLRRTLT FSPAVL LRHLSTAAAVATS SELHEDESGISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTGFSPAVLRLRHLSTAAAVATS-SELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RAVELTVQQVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 451

[Close]

BLAST of Carg18517 vs. ExPASy TrEMBL
Match: A0A5D3CMX0 (Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003690 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 4.2e-243
Identity = 428/452 (94.69%), Postives = 441/452 (97.57%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILAS LRRTLT  SP +   R +STAAAVA SPSE HEDE+GISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNIS+KGVMHFY+EKK+H+ITTQTEHKCVLDSCRHLQQEGF+ITYLPVG+
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVS+MAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RAVELTV QVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEI 452

[Close]

BLAST of Carg18517 vs. ExPASy TrEMBL
Match: A0A1S3B0Q9 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 4.2e-243
Identity = 428/452 (94.69%), Postives = 441/452 (97.57%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILAS LRRTLT  SP +   R +STAAAVA SPSE HEDE+GISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNIS+KGVMHFY+EKK+H+ITTQTEHKCVLDSCRHLQQEGF+ITYLPVG+
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPDTGLVS+MAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RAVELTV QVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEI 452

[Close]

BLAST of Carg18517 vs. ExPASy TrEMBL
Match: A0A6J1BUZ1 (Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 9.4e-243
Identity = 429/452 (94.91%), Postives = 441/452 (97.57%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASKILAS LRRTLT+ + A   LR LSTAAAV TSPSE HEDE+GISMKGVKISGRPLY
Sbjct: 1   MASKILASSLRRTLTKSTQAASLLRPLSTAAAVVTSPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVFTSGATESNNISVKGVMHFY+EKK+H+ITTQTEHKCVLDSCRHLQQEGFDITYLPVG+
Sbjct: 121 IVFTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRP+TGLVS+MAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           RA+ELTV QVEKLREMSPLYEMVKEGINIKEI
Sbjct: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEI 452

[Close]

BLAST of Carg18517 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 708.8 bits (1828), Expect = 3.8e-204
Identity = 352/452 (77.88%), Postives = 401/452 (88.72%), Query Frame = 0


Query: 1   MASKILASGLRRTLTEFSPAVLCLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60
           MASK++++ +RRTLT+        R+LSTAAA     +E++ ++  I MKGV+ISGRPLY
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQAT+P+DPRV DAM    +  YGNPHSRTHLYGWE+++AVE AR+QVA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180
           IVF SGATE+NN++VKGVMHFYK+ KKHVITTQTEHKCVLDSCRHLQQEGF++TYLPV T
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240
           DG+VDLE LR AIRPDTGLVSIMAVNNEIGV+QP+EEIG ICKE NVPFHTDAAQA+GKI
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           P+DV+KWNV+LMS+S HK+YGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  
Sbjct: 241 PVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQ 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360
           +VG GAACELAMKEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAY
Sbjct: 301 IVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLK+VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID
Sbjct: 361 VEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEI 453
           +AVELTV+QVEKLREMSPLYEMVKEGI+IK I
Sbjct: 421 KAVELTVKQVEKLREMSPLYEMVKEGIDIKNI 448

[Close]

BLAST of Carg18517 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 554.3 bits (1427), Expect = 1.2e-157
Identity = 270/320 (84.38%), Postives = 298/320 (93.12%), Query Frame = 0


Query: 133 ISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGTDGIVDLEKLRSA 192
           ++VKGVMHFYK+ KKHVITTQTEHKCVLDSCRHLQQEGF++TYLPV TDG+VDLE LR A
Sbjct: 1   MAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREA 60

Query: 193 IRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLM 252
           IRPDTGLVSIMAVNNEIGV+QP+EEIG ICKE NVPFHTDAAQA+GKIP+DV+KWNV+LM
Sbjct: 61  IRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALM 120

Query: 253 SLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAM 312
           S+S HK+YGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  +VG GAACELAM
Sbjct: 121 SMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQIVGFGAACELAM 180

Query: 313 KEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAYVEGESLLMGLKD 372
           KEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAYVEGESLLMGLK+
Sbjct: 181 KEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAYVEGESLLMGLKE 240

Query: 373 VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDRAVELTVQQVEK 432
           VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID+AVELTV+QVEK
Sbjct: 241 VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEIDKAVELTVKQVEK 300

Query: 433 LREMSPLYEMVKEGINIKEI 453
           LREMSPLYEMVKEGI+IK I
Sbjct: 301 LREMSPLYEMVKEGIDIKNI 320

[Close]

BLAST of Carg18517 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 119.4 bits (298), Expect = 9.9e-27
Identity = 86/280 (30.71%), Postives = 131/280 (46.79%), Query Frame = 0


Query: 21  VLCLRHLSTAAAVATSPSELHEDESGISM-----KGVKI------SGRPLYLDMQATSPV 80
           V C   LS  +A A S + +  D   +S+     K  +I        + +YLD  ATS  
Sbjct: 25  VRCSSSLSVCSAAAASSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQK 84

Query: 81  DPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGAS-PKEIVFTSGAT 140
              VLDA+  YY     N H   H    ++    E AR +VA  I AS  +EIVFT  AT
Sbjct: 85  PAAVLDALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNAT 144

Query: 141 ES-NNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGTDGIVDLE 200
           E+ N ++    +   K   + ++T    H C++      Q+ G  + ++ +  D + D+ 
Sbjct: 145 EAINLVAYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDIN 204

Query: 201 KLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKIPIDVEKW 260
           KLR  I P T LV++  V+N +    P+EEI     +       DA Q++  + +DV+K 
Sbjct: 205 KLRELISPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKL 264

Query: 261 NVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQ 288
           N   +  S HK+ GP GIG LY  +   +   P   GGG+
Sbjct: 265 NADFLVASSHKMCGPTGIGFLY-GKSDLLHSMPPFLGGGE 303

[Close]

BLAST of Carg18517 vs. TAIR 10
Match: AT1G27980.1 (dihydrosphingosine phosphate lyase )

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-08
Identity = 68/255 (26.67%), Postives = 113/255 (44.31%), Query Frame = 0


Query: 108 SQVAALIG----ASPKEIV--FTSGATESNNISVKGVMHFYKEKK----KHVITTQTEHK 167
           +  AAL+G    AS  +I    TSG TES  ++VK    + K KK      +I  ++ H 
Sbjct: 181 AMTAALLGSKETASGGQICGNMTSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHS 240

Query: 168 CVLDSCRHLQQEGFDITYLPVGTDGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEE 227
               + ++ +     +  +PV  D   D++  R  I  +T ++   A     G+I P+EE
Sbjct: 241 AYDKAAQYFK---IKLWRVPVDKDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEE 300

Query: 228 IGKICKEFNVPFHTDA---------AQALGK--IPIDVEKWNVSLMSLSGHKVYG--PKG 287
           +G++   + + FH D          A+ LG    P D     V+ +S+  HK YG  PKG
Sbjct: 301 LGQLALSYGICFHVDLCLGGFVLPFARKLGYQIPPFDFSVQGVTSISVDVHK-YGLAPKG 360

Query: 288 IGALYLR----RRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAMKEMEY--D 334
              +  R    R+ +     + +GG       +G+ P  LV G  AA  +++ E  Y  +
Sbjct: 361 TSTVLYRNHEIRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAM-MSLGEEGYLQN 420

The following BLAST results are available for this feature:

BLAST of Carg18517 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026904.1	0.0e+00	100.00	Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. argy...	[more]
KAG6594943.1	0.0e+00	96.23	Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023518255.1	3.2e-253	99.12	cysteine desulfurase, mitochondrial [Cucurbita pepo subsp. pepo]	[more]
XP_023003261.1	3.5e-252	99.12	cysteine desulfurase, mitochondrial [Cucurbita maxima]	[more]
XP_022963408.1	1.1e-250	99.34	cysteine desulfurase, mitochondrial [Cucurbita moschata]	[more]

BLAST of Carg18517 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49543	5.4e-203	77.88	Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=...	[more]
Q5RDE7	4.7e-167	70.45	Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1	[more]
Q9Y697	1.1e-166	70.45	Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3	[more]
Q9Z1J3	3.4e-165	69.95	Cysteine desulfurase, mitochondrial OS=Mus musculus OX=10090 GN=Nfs1 PE=1 SV=3	[more]
Q99P39	2.2e-164	69.44	Cysteine desulfurase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Nfs1 PE=2 S...	[more]

BLAST of Carg18517 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KNP3	1.7e-252	99.12	Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111496923 PE=3 SV=1	[more]
A0A6J1HHQ0	5.5e-251	99.34	Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111463622 PE=3 SV=1	[more]
A0A5D3CMX0	4.2e-243	94.69	Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]
A0A1S3B0Q9	4.2e-243	94.69	Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1	[more]
A0A6J1BUZ1	9.4e-243	94.91	Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1	[more]

BLAST of Carg18517 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65720.1	3.8e-204	77.88	nitrogen fixation S (NIFS)-like 1	[more]
AT5G65720.2	1.2e-157	84.38	nitrogen fixation S (NIFS)-like 1	[more]
AT1G08490.1	9.9e-27	30.71	chloroplastic NIFS-like cysteine desulfurase	[more]
AT1G27980.1	2.1e-08	26.67	dihydrosphingosine phosphate lyase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 60..421 e-value: 1.7E-151 score: 506.3
IPR000192	Aminotransferase class V domain	PFAM	PF00266	Aminotran_5	coord: 60..421 e-value: 6.5E-87 score: 291.9
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 70..315 e-value: 1.7E-151 score: 506.3
IPR010240	Cysteine desulfurase IscS	TIGRFAM	TIGR02006	TIGR02006	coord: 57..453 e-value: 1.5E-195 score: 647.9
IPR010240	Cysteine desulfurase IscS	PANTHER	PTHR11601:SF34	CYSTEINE DESULFURASE, MITOCHONDRIAL	coord: 49..443
IPR010240	Cysteine desulfurase IscS	HAMAP	MF_00331	Cys_desulf_IscS	coord: 55..448 score: 52.718922
None	No IPR available	PANTHER	PTHR11601	CYSTEINE DESULFURYLASE FAMILY MEMBER	coord: 49..443
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 58..444

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18517-RA	Carg18517-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044571	[2Fe-2S] cluster assembly
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0031071	cysteine desulfurase activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity