Carg18505 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18505
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tRNA-dihydrouridine synthase
Location	Carg_Chr07: 2668045 .. 2673115 (-)
RNA-Seq Expression	Carg18505
Synteny	Carg18505

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGTCCAGCCCATTGTGAACTCTGTTCTACATTTCAGTTTTCCGGCCTACGGTTAGCCGGAGGAGAAGACGTAGAAATAGTTGCTTGATTTTCTGGTCCGACCGCTCACGGTTGAGATGGATTACGAGAACAAGCTCGTCCTTGCTCCCATGGTTCGCGTAGTAAGTTCGACGAAATTATTGTATCCACTGCTTGAGTTGTTCTTGTTAGTTAGTCGAGTATCTCTCTTAATCTTAGGAGTTATTTATTGCAAATTTCTCGCTCGATTGTTTCGGTTTAGATGTCAAGCTATGTTACGAGATTTAGCTTAGTTCGTATTGTTTAGGTTCCTCTGCTTGCTTTATCAGTATATATTTCAATCGTAGTTATTTCTTGCAAAATTCCCGCCAATTAAATCTTAATCGTGTAGTCTTTTGTGATTGGTCCTTCTTGTTTTCAGGGGACTTTGCCTTTTAGATTATTAGCTGCACAATACGGTGCAGACATCACCTATGGAGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGAATAAATGGTACTTTTCCTCCTCTCTATTGTGGAAGTTTTTTTTAAGGTATTGGTTTTCAATTGTACTTTCTCACTAAGTGCAAAAATCATGAAATACAAGTGTTACTATGTTCGTATGAAGTCTTTTTCACTAAGAAAAGCTTTGCGATATGAACGGACACAAAAATGAACGCAGGCAGTGGAAATGCACCCACCTAGAAAAACTCGCCTTTATGTTAGGGGCACTAAAATGGAACTTCGATCTGATAAAATGATCTGTTGACTTCAACCATAACTTCGAAATTGGGAGAAATCTAGGCCTTTTCTCATTCTCTATTGATATTTATGAATGTGTTTGTTGGACACTATCATTTATCCATCTTTCAATTATTTATCCAAAGCCCAAACAATATGTTGGAATTTTTACCGTTCTTCTATAAATACGAGTTCAATTAGAAGAAAAAATGTTGTGCTTGGTTAGTGGCTGAGAAAATGAAGTTCCCAATAAGCATAGCCTTTTCCTTTTTCTTCACCAATTATAAAACTTCTGATACTTCTCTTTGTCATCTAGAGCACGTTGGTGCAGTCGATTTCGTAGAAAAGGGGACAGACAATGTTGTTTTCAGGACTTGCAGCCTAGAAAAAAATGAAGTTGTTTTTCAAATGGGTACTTCTAATGCTGTGAGGGCTTTAAGTGCTGCTCAACTAGTGTAAGTTCGTTACTCCCAATTTTTCTTTGATTATACAGCCTAATTCTTGCAAGTTTGATGCTTTTATGATCCTGCGAGTATCAAGTCCCAGCCTGCCTGTTCCTTACTTTGCACAAATTTGTAATCATTTTTTTTATCCTGCAACTAAGTGTCTTTATTAATAGGCTCTGTCATAGATCGAACTAAATTTTGGTAGTACTTTTACTATTTTTTAAAAGTTATTCAATTAACGGGATGGACATGATTTTTCTTTCTTAACAGGCTCAGTTATGGATAGAACTTAATTTTTAGTCGCTAGTTCTCTTTTTTATTCAATTTCATGCGATGCTCGTGATTACGTTGTGAGATTCTACTGGCTAAGTTGATTATTTCCTGTTCTCTTTCATTGTGGGTTTTGATTAGGTACTAATTGGGAACAATTAAGAAATTTTCAGTCCGATTTCTTACAAAATGAAACCTATTAATTGATTAGGCATAGATTGCTACTCAAGATTGGATGTGGGTAAATGGTCTATTTTGTGTGAATAAACAGTATGATAGGTTTTTGTTCCTAGAAACTGTTTGTTTTCTTTGTCTTTCCCTCGGCTATAGGCTTTTAGTTAAGGTTTAGCCAAACTTGTTCAGTTCGTCTGCCAAAGCAACATTCAGTGGTTGCTATTATGACAGATGGAACCTGTGGTACAAAATTTTGGCAAATTAAGAGAGAGTTCCTATTCAATTTGGCTGTAAGAATGGGTTATTTATTGATGCTTTTAAATGTTCTCTTAGTAACTGAAAGTTGATTTCTGTGAGTTGTTGCATTATATTGCTAGGTTTTGTGGTGCAAGCTATTTGTACTTCAGCGTTCTCTGTTTTATTGCTTTGCTCGGTTTACTTATTATAAGTCATGTGTCATCTTTGTACTATATTGTTGTGGAAGTATATCCTTTCTTTAGGCCCTATTTGATAATCATTTTGTTTTTGGTTTTCTGATTTTGAAAATTAAATCTGTAAACACTGTTTTCACATATACTTTTTTTTTCTTTATCACCTATTTTCTAAAAACATTTAAAAGTTCCAACCCAAATTTTGGAAACCAAAAATAAAGTAGTGTTAAAAAGCTTGATTTTGCTTTTGAAATTTTGTCTAGAAATCAAATGTGTTGTTAAAGAAGGAGAAAAACTTACTAAAAACTCGTGAGGAGACGCTCAGTAATTTTTTAAATGCAGAAAACCAAAAATAAAGCTGTTTTCGAACCGGACCTTAGTTATTTCAAGCTGATTGAATTGACATTGTTTATGATATGAGAGAATTAGGTCTTTAGTTAGAGAAGAATGTTTCTTTAATTACCATTGTTTGTGATGGAGAATTTTCCATTTTAGGTGTCAAGACGTTGCTGCAATAGATATCAATATGGGTTGCCCTAAGTCATTTTCCATCAGTGGGGGCATGGGTGCTGCACTGTTGAAGAAGCCAGACCTTATTCATGATGTATGCAATTCTTATCGTTATGAATGATATAGTTTTCTAAATTATTCTCTCTCTTCTATATTTGTAACATGTTACTTACCTATTCTCCTTTTATTTGTATGTTTTTTTAATTTAGATTTTGACAACATTGAGGAGGAACTTGGACATTCCAATTACCTGTAAGATTCGACTTTTAAATTCATCTCAGGATACAGTGGAGTTAGCTAGGCGAATAGAGACTACTGGCGTTTCAGCTCTTGCTGTTCATGGAAGGTGATCTTCTGGTTTCTGCAGTTTCCTTATATTTAATACTATCATGGACTAGCGAACTTCTTCAAGAAATTTATGCTTTCTGGAATCGATTGAGAATGGTTCTTCTCATGCAAATTGATCCTTTTTCCCTTTCTTGGTAAAATTGGATTGGTTTGGTTGTCTATGTTAGTGCATTGTTCTCAAAGTTAACATGGTCATTTCATTTTAAGCCCACACTGACATTGTTGACGTTTTCTTAAACATGCTATCTGAGAGTGGAGGTGATAAATATGTATCAACCTTAAAAGATTGAATTCTGTTGTACAGAACAATGAAAGAAAGTCTCAAACGGTAGAAGCAGATATGTCATATGTCCGTTCACTAGCTAGTTATTTAGATGTAATTTTGTGGACGTTCATTAGCTAGAAACTATTAGATATAATTTCATGCTTTAAGGAGGGTAGTAATTTGGACGAGTCTCTTGCATTTCAAGGCTTACAGTTTTTTTTTTTCATAAGGGGATAGTAGGTTTGAGAGATTTCTAGGAAGCAGAATATGTGCTACCAAGCATGCTCAGAGGGCAGACTACTGTGAGAGGCCGCTTGTTTATAAGTAAAGGAGGCAGTTGGTATCATTTTGGGTTGACACTGAATTGTATTACATCAGTTAGAAAGACAAAAGGAAAAATAAAAAATAAAAAATACCGAGTTGTATTTCATCAGACCTTTTAGTTCCAAGTGATACTTCTGTAAGTTTTGTATGTGCTCTTTGTTTTCATTTTATTTTAATATGTTTTTGTAGTCGTGCTCGATAACTTGCAGAAGAGTAGCAGATAGGCCCAGAGATCCAGCTAAGTGGAGTGAGATTGCAGATGTTGTGGCAGCATTGTCTGTCCCGGTTATTGCGAACGGTGATATCTTTGAGTACGAAGATTTTCACCATATCAAAGTTGCTACAGGTTTGCTCAAAAGGCACTGTTTTATGGATTTCTTGATGATGCCTGAATTTCTTTTAGTGAATGGGTTGAATATGATCCCAACCTGAAAATATAACTTAACTAAGATTAAAAGTTTGAACTCTCATTCCTGCATATATGGTTGAACTCTATATTATTTGAATATGATCGTTTCGAATAATATGGTTGGGCTGACTGAGATGCTATTTCAAGCAGTTAAAGTTTTTAGTTGTCCGTTCATGATCTCTTGCTTTATATATTAAAACAATTTGGTATTGCTCAAGGTGCTTCGTCAGTAATGGTTGCTCGAGGAGCGCTTTGGAACGCTTCAATCTTCTCTGCAGATGGCAAATTACCATGGGAAGAGGTGAAAAGAGAATATGTTAGAAAGGTGAGGTACATATCATGTACGCAGTACATTAGTCAACGAATCAATTAGAGTTGATGCTGTAGAGCTGTACACTACCCTTGTAAGAATATATAGATGCAGACACACATTCACGAACTGAATTTGAAGCGACTGATTTGTTGTGTTGAACTCAGAGCATCTTGTGGGACAATGATATCAAAAGTACAAAGCACACGCTAAAAGAAATGATAATGCATTATTCTTGCCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGATACATTGGCAGATCTAGCGTAAGTTTTTTTCTTTCTTTTTTTGCTGTTAATTTATAATTAATAGTGTGCCATCTTGTGGGATACTAGACGTTCTTCATGATCATTTTAATTTTAATTGCATTTGAACTGATATAGCACGCTAGTTGGAAGCTAATCATGCCATCTTTTATTTTGTGTATGATATATAGGAAACTTTATGGTGAGGAAAAGTACAATCAATTTGTTCAAGAGAAGCGTTTGTTCGATGGAAGTAGATGACGTCGTTCGTAGTTCGGATCAGAGACGCTGCTCATTGCTTCGAAATGGAACAACCTCGGTACTCTCTCAGTTAAACCCGTTTTATTAGTTATGGAGAAGTTGGATGTGCTCTACAGCAGCTCAATACTAACATATTTCTTACCCTTTCATTCTTGTCGTGTTAAAAACACAGAGTTATTAAGCTGCCGCAGATGATGCCTGGCTTTATTTGCTTTTCTGGAATGCGTATTTATGGTTCTAGGATGTTCCAAGTTGAAAGCTTTGGCTTATTGTTGCC

mRNA sequence

AAGTCCAGCCCATTGTGAACTCTGTTCTACATTTCAGTTTTCCGGCCTACGGTTAGCCGGAGGAGAAGACGTAGAAATAGTTGCTTGATTTTCTGGTCCGACCGCTCACGGTTGAGATGGATTACGAGAACAAGCTCGTCCTTGCTCCCATGGTTCGCGTAGGGACTTTGCCTTTTAGATTATTAGCTGCACAATACGGTGCAGACATCACCTATGGAGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGAATAAATGAGCACGTTGGTGCAGTCGATTTCGTAGAAAAGGGGACAGACAATGTTGTTTTCAGGACTTGCAGCCTAGAAAAAAATGAAGTTGTTTTTCAAATGGGTACTTCTAATGCTGTGAGGGCTTTAAGTGCTGCTCAACTAGTGTGTCAAGACGTTGCTGCAATAGATATCAATATGGGTTGCCCTAAGTCATTTTCCATCAGTGGGGGCATGGGTGCTGCACTGTTGAAGAAGCCAGACCTTATTCATGATATTTTGACAACATTGAGGAGGAACTTGGACATTCCAATTACCTGTAAGATTCGACTTTTAAATTCATCTCAGGATACAGTGGAGTTAGCTAGGCGAATAGAGACTACTGGCGTTTCAGCTCTTGCTTCGTGCTCGATAACTTGCAGAAGAGTAGCAGATAGGCCCAGAGATCCAGCTAAGTGGAGTGAGATTGCAGATGTTGTGGCAGCATTGTCTGTCCCGGTTATTGCGAACGGTGATATCTTTGAGTACGAAGATTTTCACCATATCAAAGTTGCTACAGGTGCTTCGTCAGTAATGGTTGCTCGAGGAGCGCTTTGGAACGCTTCAATCTTCTCTGCAGATGGCAAATTACCATGGGAAGAGGTGAAAAGAGAATATGTTAGAAAGAGCATCTTGTGGGACAATGATATCAAAAGTACAAAGCACACGCTAAAAGAAATGATAATGCATTATTCTTGCCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGATACATTGGCAGATCTAGCGAAACTTTATGGTGAGGAAAAGTACAATCAATTTGTTCAAGAGAAGCGTTTGTTCGATGGAAGTAGATGACGTCGTTCGTAGTTCGGATCAGAGACGCTGCTCATTGCTTCGAAATGGAACAACCTCGGTACTCTCTCAGTTAAACCCGTTTTATTAGTTATGGAGAAGTTGGATGTGCTCTACAGCAGCTCAATACTAACATATTTCTTACCCTTTCATTCTTGTCGTGTTAAAAACACAGAGTTATTAAGCTGCCGCAGATGATGCCTGGCTTTATTTGCTTTTCTGGAATGCGTATTTATGGTTCTAGGATGTTCCAAGTTGAAAGCTTTGGCTTATTGTTGCC

Coding sequence (CDS)

ATGGATTACGAGAACAAGCTCGTCCTTGCTCCCATGGTTCGCGTAGGGACTTTGCCTTTTAGATTATTAGCTGCACAATACGGTGCAGACATCACCTATGGAGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGAATAAATGAGCACGTTGGTGCAGTCGATTTCGTAGAAAAGGGGACAGACAATGTTGTTTTCAGGACTTGCAGCCTAGAAAAAAATGAAGTTGTTTTTCAAATGGGTACTTCTAATGCTGTGAGGGCTTTAAGTGCTGCTCAACTAGTGTGTCAAGACGTTGCTGCAATAGATATCAATATGGGTTGCCCTAAGTCATTTTCCATCAGTGGGGGCATGGGTGCTGCACTGTTGAAGAAGCCAGACCTTATTCATGATATTTTGACAACATTGAGGAGGAACTTGGACATTCCAATTACCTGTAAGATTCGACTTTTAAATTCATCTCAGGATACAGTGGAGTTAGCTAGGCGAATAGAGACTACTGGCGTTTCAGCTCTTGCTTCGTGCTCGATAACTTGCAGAAGAGTAGCAGATAGGCCCAGAGATCCAGCTAAGTGGAGTGAGATTGCAGATGTTGTGGCAGCATTGTCTGTCCCGGTTATTGCGAACGGTGATATCTTTGAGTACGAAGATTTTCACCATATCAAAGTTGCTACAGGTGCTTCGTCAGTAATGGTTGCTCGAGGAGCGCTTTGGAACGCTTCAATCTTCTCTGCAGATGGCAAATTACCATGGGAAGAGGTGAAAAGAGAATATGTTAGAAAGAGCATCTTGTGGGACAATGATATCAAAAGTACAAAGCACACGCTAAAAGAAATGATAATGCATTATTCTTGCCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGATACATTGGCAGATCTAGCGAAACTTTATGGTGAGGAAAAGTACAATCAATTTGTTCAAGAGAAGCGTTTGTTCGATGGAAGTAGATGA

Protein sequence

MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVEKGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSITCRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

Homology

[Close]

BLAST of Carg18505 vs. NCBI nr
Match: KAG7026892.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 655.2 bits (1689), Expect = 2.9e-184
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
           CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW
Sbjct: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 328

[Close]

BLAST of Carg18505 vs. NCBI nr
Match: XP_022962639.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 635.6 bits (1638), Expect = 2.4e-178
Identity = 322/328 (98.17%), Postives = 323/328 (98.48%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA   + 
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 325

[Close]

BLAST of Carg18505 vs. NCBI nr
Match: KAG6594928.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 632.9 bits (1631), Expect = 1.6e-177
Identity = 321/328 (97.87%), Postives = 323/328 (98.48%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA   + 
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFH+IKVATGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHNIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 325

[Close]

BLAST of Carg18505 vs. NCBI nr
Match: XP_023003695.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 632.5 bits (1630), Expect = 2.0e-177
Identity = 320/328 (97.56%), Postives = 322/328 (98.17%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKN+VVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNQVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA   + 
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFS DGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSEDGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 325

[Close]

BLAST of Carg18505 vs. NCBI nr
Match: XP_023517246.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.2 bits (1624), Expect = 1.0e-176
Identity = 319/328 (97.26%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAA YGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAHYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKN+VVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNQVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA   + 
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFSADGKLPWEEVKREYVRKSI WDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSADGKLPWEEVKREYVRKSISWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 325

[Close]

BLAST of Carg18505 vs. ExPASy Swiss-Prot
Match: Q9NX74 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Homo sapiens OX=9606 GN=DUS2 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.7e-79
Identity = 159/312 (50.96%), Postives = 210/312 (67.31%), Query Frame = 0


Query: 3   YENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVEKG 62
           Y NKL+LAPMVRVGTLP RLLA  YGADI Y EE+ID KMI+C+R +NE +  VDFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAP- 69

Query: 63  TDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGMGA 122
            D VVFRTC  E+N VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  DDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGA 129

Query: 123 ALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSITCR 182
           ALL  PD I  IL+TL +    P+TCKIR+L S +DT+ L +RIE TG++A+A   +  R
Sbjct: 130 ALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIA---VHGR 189

Query: 183 RVADRPRDPAKWSEIADVVAALSVPVIANG----DIFEYEDFHHIKVATGASSVMVARGA 242
           +  +RP+ P     I  +   LS+PVIANG     I +Y D    + AT ASSVMVAR A
Sbjct: 190 KREERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAA 249

Query: 243 LWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIK 302
           +WN SIF  +G  P EEV ++Y+R ++ +DN   +TK+ L +M+     LE P+G+ +  
Sbjct: 250 MWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHA 309

Query: 303 SDTLADLAKLYG 311
           + +  ++ + +G
Sbjct: 310 AQSSREICEAFG 315

[Close]

BLAST of Carg18505 vs. ExPASy Swiss-Prot
Match: Q9D7B1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN=Dus2 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.3e-78
Identity = 158/312 (50.64%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 3   YENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVEKG 62
           Y NKL+LAPMVRVGTLP RLLA  YGADI Y EE+ID KM++C+R +NE +  VDFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMLQCKRVVNEVLSTVDFVAP- 69

Query: 63  TDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGMGA 122
            D VVFRTC  E++ VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  DDRVVFRTCEREQSRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKEYSTKGGMGA 129

Query: 123 ALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSITCR 182
           ALL  PD I  IL+TL +    P+TCKIR+L S +DT+ L +RIE TG+SA+A   +  R
Sbjct: 130 ALLSDPDKIEKILSTLVKGTHRPVTCKIRILPSLEDTLNLVKRIERTGISAIA---VHGR 189

Query: 183 RVADRPRDPAKWSEIADVVAALSVPVIANG----DIFEYEDFHHIKVATGASSVMVARGA 242
              +RP+ P     I  +   LS+PVIANG     I ++ D    + AT ASSVMVAR A
Sbjct: 190 NRDERPQHPVSCEVIRAIAETLSIPVIANGGSHDHIQQHVDIEDFRQATAASSVMVARAA 249

Query: 243 LWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIK 302
           +WN SIF  DG  P EEV ++Y+R ++ +DN   +TK+ L +M+     LE P+G+ +  
Sbjct: 250 MWNPSIFLKDGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHA 309

Query: 303 SDTLADLAKLYG 311
           + +  ++ + +G
Sbjct: 310 AQSSQEICEAFG 315

[Close]

BLAST of Carg18505 vs. ExPASy Swiss-Prot
Match: O74731 (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus2 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.3e-53
Identity = 113/292 (38.70%), Postives = 176/292 (60.27%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIK---CERRINEHVGAVD 60
           ++Y NK+ LAPMVR+G LP RLLA +YGA++ +G EI+D  ++     ER +N+ +  +D
Sbjct: 4   LNYSNKVCLAPMVRIGELPMRLLALRYGANLVWGPEIVDKALLSGTPVERVVNDRINCID 63

Query: 61  FVEKGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSIS 120
           FV+  ++ V+FR   LE N ++FQ+G+++   A+ AA+LV  DVA ID+N GCPK FS+ 
Sbjct: 64  FVKPPSNKVLFRVHPLEANRLIFQLGSASPELAVEAAKLVANDVAGIDLNCGCPKHFSVH 123

Query: 121 GGMGAALLKKPDLIHDILTTLRRNLDIP----ITCKIRLLNSSQDTVELARRIETTGVSA 180
            GMGA LLK  D +  IL  L   +  P    I+CKIRLL + +DT++L  RI  TGV A
Sbjct: 124 AGMGAGLLKNQDRLVSILDALVNEIGKPYKISISCKIRLLETKEDTLKLVERICDTGVRA 183

Query: 181 LASCSITCRRVADRPRDPAKWSEIADVVAAL---SVPVIANGDIFEYEDFHHIKVATGAS 240
           +   ++ CR    R  +PA  S ++++V       V ++ NGD+  Y D   +    G  
Sbjct: 184 I---TVHCRTTPMRNTEPADRSYLSEIVGVCRNKDVSILVNGDVLSYNDGLDVIEKYGVD 243

Query: 241 SVMVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMI 283
            V++AR A  N S F  +G L   +V  E+++ ++  DN+  +TK+ L +++
Sbjct: 244 GVLIARAAERNVSCFRIEGPLSSFKVAEEFLKMALEVDNNFGNTKYCLNQIM 292

[Close]

BLAST of Carg18505 vs. ExPASy Swiss-Prot
Match: P53720 (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SMM1 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.9e-43
Identity = 114/302 (37.75%), Postives = 176/302 (58.28%), Query Frame = 0


Query: 3   YENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDF---- 62
           Y  KLVLAPMVR G LP RL+A  +GAD+ +  EIID K+I+C R+ N  +  VD+    
Sbjct: 4   YAGKLVLAPMVRAGELPTRLMALAHGADLVWSPEIIDKKLIQCVRKENTALQTVDYVVPS 63

Query: 63  -VEKGTDNVVFRTC-SLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSI 122
            V+   + +VFRT   LE ++++FQ+G+++   A  AA  V  DV+ IDIN GCPK FSI
Sbjct: 64  KVQTRPETLVFRTYPKLESSKLIFQIGSASPALATQAALKVINDVSGIDINAGCPKHFSI 123

Query: 123 SGGMGAALLKKPDLIHDILTTLRRNL----DIPITCKIRLLNSSQDTVELARRIETTGVS 182
             GMG+ALL+ PD +  IL  L +N+      PI+ KIRLL++ QDT++L +R+  TG++
Sbjct: 124 HSGMGSALLRTPDTLCLILKELVKNVGNPHSKPISVKIRLLDTKQDTLQLVKRLCATGIT 183

Query: 183 ALASCSITCRRVADRPRDPA---KWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGA 242
            L   ++ CR+   R R+       +EI ++  A +V +I NG I +   FH ++     
Sbjct: 184 NL---TVHCRKTEMRNREQPITDYIAEIYEICQANNVSLIVNGAIRDRSHFHDLQANHWK 243

Query: 243 SS---VMVARGALWNASIF------SADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKE 283
           ++    M+A  A  + ++F      S DG   W    RE+++ +  +DN I +TK+ L  
Sbjct: 244 NTNIGGMIAECAERDPTVFDHTSKPSEDGP-SWVVACREFIQWATKFDNHIGNTKYMLSR 301

[Close]

BLAST of Carg18505 vs. ExPASy Swiss-Prot
Match: Q32M08 (tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN=Dus4l PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.9e-27
Identity = 81/252 (32.14%), Postives = 128/252 (50.79%), Query Frame = 0


Query: 8   VLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVEKGTDNVV 67
           V APMVR   L FR L  +Y  D+ Y   II                A DFV     ++ 
Sbjct: 30  VCAPMVRYSKLAFRTLVRKYSCDLCYTPMII----------------AADFVR----SIK 89

Query: 68  FRTCSLEKNE----VVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAA 127
            R      N+    ++ Q   ++A     AA LVC     IDIN GCP+ ++++ G GA 
Sbjct: 90  ARDSEFTTNQGDCPLIVQFAANDARLLSDAALLVCPYANGIDINCGCPQRWAMADGYGAC 149

Query: 128 LLKKPDLIHDILTTLRRNLDIP---ITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 187
           L+ KP+L+HD++  +R  ++ P   ++ KIR+ +    T++L R+ E TGVS +   ++ 
Sbjct: 150 LINKPELVHDMVRQVRNRVESPRFSVSIKIRIHDDLARTIDLCRKAEATGVSWI---TVH 209

Query: 188 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 247
            R V +R   P  +  I  +   +S+P++ANGDI   ++  ++   TG   VMVARG L 
Sbjct: 210 GRTVEER-HQPVHYDAIKMIKENVSIPIVANGDIRSLKEAENVWQMTGTDGVMVARGLLA 257

Query: 248 NASIFSADGKLP 253
           N ++F+   + P
Sbjct: 270 NPAMFAGYEETP 257

[Close]

BLAST of Carg18505 vs. ExPASy TrEMBL
Match: A0A6J1HHN5 (tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 322/328 (98.17%), Postives = 323/328 (98.48%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA   + 
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 325

[Close]

BLAST of Carg18505 vs. ExPASy TrEMBL
Match: A0A6J1KSI8 (tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 9.9e-178
Identity = 320/328 (97.56%), Postives = 322/328 (98.17%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKN+VVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNQVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA   + 
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFS DGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSEDGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 325

[Close]

BLAST of Carg18505 vs. ExPASy TrEMBL
Match: A0A6J1BSD4 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005266 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.6e-164
Identity = 292/328 (89.02%), Postives = 312/328 (95.12%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           M+YENKL+LAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKM+KCERR+NE VG+ DFVE
Sbjct: 1   MEYENKLILAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMVKCERRLNERVGSTDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLE+++VVFQMGTSNAVRAL+AAQ+VCQDVAA+DINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLERSQVVFQMGTSNAVRALTAAQIVCQDVAAVDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALL KPDLIHDILTTLRRNL+IPITCKIRLL+SSQDTVELARRIET GVSAL    + 
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNIPITCKIRLLSSSQDTVELARRIETIGVSALV---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALS+PVIANGDIFEY DF  +KV TGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSIPVIANGDIFEYADFQRVKVDTGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFS +GKLPWE+VKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKS+
Sbjct: 241 NASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSE 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           +LADLAKLYGEEKYN FVQEKRLFDGSR
Sbjct: 301 SLADLAKLYGEEKYNLFVQEKRLFDGSR 325

[Close]

BLAST of Carg18505 vs. ExPASy TrEMBL
Match: A0A6J1HFN6 (tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 2.9e-161
Identity = 298/328 (90.85%), Postives = 299/328 (91.16%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALLKKPDLIHD                        DTVELARRIETTGVSALA   + 
Sbjct: 121 GAALLKKPDLIHD------------------------DTVELARRIETTGVSALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD
Sbjct: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           TLADLAKLYGEEKYNQFVQEKRLFDGSR
Sbjct: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 301

[Close]

BLAST of Carg18505 vs. ExPASy TrEMBL
Match: A0A1S3B193 (tRNA-dihydrouridine synthase OS=Cucumis melo OX=3656 GN=LOC103484905 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 2.9e-161
Identity = 289/328 (88.11%), Postives = 308/328 (93.90%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLA+QYGADITYGEEIIDHKMIKCERR+NEHVG+ DFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTCSLE+  VVFQMGTSNA RALSAAQLVC+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALL KPDLIHDILTTLRRNLD+ ITCKIRLLNS QDTVELARRIETTGVSAL    + 
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSAL---GVH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            RRVADRPRDPAKWSEIADVVAAL++PVIANGDIFEY+DF  IKV TGASS+MVARGALW
Sbjct: 181 GRRVADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFS +GKLPWE+VKREYVRKSILWDNDIKSTK+TLKEMIMH+SCLELPEGKAVIKS+
Sbjct: 241 NASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSE 300

Query: 301 TLADLAKLYGEEKYNQFVQEKRLFDGSR 329
           +LADLAKLYGEEKY QFVQE R F+GSR
Sbjct: 301 SLADLAKLYGEEKYYQFVQENRSFNGSR 325

[Close]

BLAST of Carg18505 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 482.6 bits (1241), Expect = 2.4e-136
Identity = 234/314 (74.52%), Postives = 272/314 (86.62%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           MDY+NKLVLAPMVRVGTL FR+LAA+YGADITYGEEIIDHK++KCERR+N   G  +FVE
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 61  KGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTDNVVF TC  EK+ VVFQMGTS+AVRAL A+++VC DVA IDINMGCPK+FSI GGM
Sbjct: 61  KGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGM 120

Query: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSIT 180
           GAALL KP+LIHDIL TL+RNLD+P+TCKIRLL S  DTVELARRIE  GV ALA   + 
Sbjct: 121 GAALLSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALA---VH 180

Query: 181 CRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALW 240
            R++ADRPRDPAKW EIADVVAALS+PVIANGD+ EY+DF  IK ATGA+SVMVARGA+W
Sbjct: 181 GRKIADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMW 240

Query: 241 NASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSD 300
           NASIFS  GK  WE+VK++Y+RKSILW+ND+KSTK+T+KEMI H+SCLELPEGK++ K+D
Sbjct: 241 NASIFSPKGKSHWEDVKKKYLRKSILWNNDVKSTKYTIKEMIAHHSCLELPEGKSINKAD 300

Query: 301 TLADLAKLYGEEKY 315
           TL DLA+LY  E Y
Sbjct: 301 TLEDLARLYDLEDY 311

[Close]

BLAST of Carg18505 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 391.7 bits (1005), Expect = 5.7e-109
Identity = 191/261 (73.18%), Postives = 223/261 (85.44%), Query Frame = 0


Query: 54  GAVDFVEKGTDNVVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKS 113
           G  +FVEKGTDNVVF TC  EK+ VVFQMGTS+AVRAL A+++VC DVA IDINMGCPK+
Sbjct: 15  GTSEFVEKGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKA 74

Query: 114 FSISGGMGAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSA 173
           FSI GGMGAALL KP+LIHDIL TL+RNLD+P+TCKIRLL S  DTVELARRIE  GV A
Sbjct: 75  FSIQGGMGAALLSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPA 134

Query: 174 LASCSITCRRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVM 233
           LA   +  R++ADRPRDPAKW EIADVVAALS+PVIANGD+ EY+DF  IK ATGA+SVM
Sbjct: 135 LA---VHGRKIADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVM 194

Query: 234 VARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEG 293
           VARGA+WNASIFS  GK  WE+VK++Y+RKSILW+ND+KSTK+T+KEMI H+SCLELPEG
Sbjct: 195 VARGAMWNASIFSPKGKSHWEDVKKKYLRKSILWNNDVKSTKYTIKEMIAHHSCLELPEG 254

Query: 294 KAVIKSDTLADLAKLYGEEKY 315
           K++ K+DTL DLA+LY  E Y
Sbjct: 255 KSINKADTLEDLARLYDLEDY 272

[Close]

BLAST of Carg18505 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 6.0e-26
Identity = 78/254 (30.71%), Postives = 121/254 (47.64%), Query Frame = 0


Query: 6   KLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVEKGTDN 65
           K ++APMV    LPFRLL  +YGA   Y   +      + E+  N+              
Sbjct: 89  KYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQE------------- 148

Query: 66  VVFRTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAALL 125
             F TC  E   +  Q   ++    L AA+ V      +DIN+GCP+  +  G  GA L+
Sbjct: 149 --FTTCK-EDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGAFLM 208

Query: 126 KKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALASCSITCRRVA 185
               L+  ++  L +NL++P++CKIR+  + +DT++ A+ +E  G S LA    T R   
Sbjct: 209 DNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRT-RDEK 268

Query: 186 DRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALWNASIF 245
           D  +  A WS I +V  A+ +PV+ANG++   ED  +    TG   V+ A   L N + F
Sbjct: 269 DGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENPAAF 325

Query: 246 SADGKLPWEEVKRE 260
           +      W +   E
Sbjct: 329 AGFRTAEWAKDNEE 325

[Close]

BLAST of Carg18505 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.6e-18
Identity = 88/312 (28.21%), Postives = 136/312 (43.59%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60
           +D+ +KL LAP+  VG LPFR L    GAD+T GE      M  C   +         + 
Sbjct: 334 IDFRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 393

Query: 61  KGTDNVVF--RTCSLEKNEVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISG 120
           + +   +F  + C    + V          R +      C  V  IDINMGCP    ++ 
Sbjct: 394 RHSSEDLFGVQICGSYPDTV---------SRVVELIDREC-TVDFIDINMGCPIDMVVNK 453

Query: 121 GMGAALLKKPDLIHDILTTLRRNLDIPITCKIR--LLNSSQDTVELARRIETTGVSALAS 180
             G+ALL KP  + +I+      ++ PIT K+R            L   I   G +A+  
Sbjct: 454 SAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAV-- 513

Query: 181 CSITCRRVADRPRDPAKWSEIADVV--AALSVPVIANGDIFEYEDFH-HIKVATGASSVM 240
            +I  R    R    A W  I      A  ++ VI NGD++ Y D++ H       SS M
Sbjct: 514 -TIHGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCM 573

Query: 241 VARGALWNASIFS-ADGKLPWE-------EVKREYVRKSIL-WDND---IKSTKHTLKEM 294
           +ARGAL    IF+    +  W+        + +++VR  +  W +D   +++T+H L E 
Sbjct: 574 IARGALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEW 625

The following BLAST results are available for this feature:

BLAST of Carg18505 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026892.1	2.9e-184	100.00	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein [Cucurbita argyrosperma ...	[more]
XP_022962639.1	2.4e-178	98.17	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita moschata]	[more]
KAG6594928.1	1.6e-177	97.87	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein, partial [Cucurbita argy...	[more]
XP_023003695.1	2.0e-177	97.56	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita maxima]	[more]
XP_023517246.1	1.0e-176	97.26	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita pepo subsp...	[more]

BLAST of Carg18505 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9NX74	2.7e-79	50.96	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Homo sapiens OX=9606 GN=DUS2 ...	[more]
Q9D7B1	2.3e-78	50.64	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN=Dus2...	[more]
O74731	3.3e-53	38.70	tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Schizosaccharomyces pombe (strain ...	[more]
P53720	6.9e-43	37.75	tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain A...	[more]
Q32M08	6.9e-27	32.14	tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN...	[more]

BLAST of Carg18505 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HHN5	1.2e-178	98.17	tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 ...	[more]
A0A6J1KSI8	9.9e-178	97.56	tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV...	[more]
A0A6J1BSD4	1.6e-164	89.02	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 OS=Momordica charanti...	[more]
A0A6J1HFN6	2.9e-161	90.85	tRNA-dihydrouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111463056 PE=3 ...	[more]
A0A1S3B193	2.9e-161	88.11	tRNA-dihydrouridine synthase OS=Cucumis melo OX=3656 GN=LOC103484905 PE=3 SV=1	[more]

BLAST of Carg18505 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49640.1	2.4e-136	74.52	Aldolase-type TIM barrel family protein	[more]
AT3G49640.2	5.7e-109	73.18	Aldolase-type TIM barrel family protein	[more]
AT5G67220.1	6.0e-26	30.71	FMN-linked oxidoreductases superfamily protein	[more]
AT4G38890.1	1.6e-18	28.21	FMN-linked oxidoreductases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001269	tRNA-dihydrouridine synthase	PIRSF	PIRSF006621	Dus	coord: 1..326 e-value: 8.4E-53 score: 177.4
IPR035587	DUS-like, FMN-binding domain	PFAM	PF01207	Dus	coord: 8..285 e-value: 5.6E-53 score: 180.0
IPR035587	DUS-like, FMN-binding domain	CDD	cd02801	DUS_like_FMN	coord: 6..253 e-value: 5.03274E-90 score: 266.668
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..251 e-value: 1.4E-73 score: 249.1
None	No IPR available	PIRSR	PIRSR006621-1	PIRSR006621-1	coord: 3..261 e-value: 1.9E-52 score: 175.9
None	No IPR available	PANTHER	PTHR45936	TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE	coord: 1..319
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 5..284
IPR018517	tRNA-dihydrouridine synthase, conserved site	PROSITE	PS01136	UPF0034	coord: 104..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18505-RA	Carg18505-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002943	tRNA dihydrouridine synthesis
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0017150	tRNA dihydrouridine synthase activity
molecular_function	GO:0003824	catalytic activity