Carg18475 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg18475 vs. NCBI nr
Match: KAG7010395.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 887.1 bits (2291), Expect = 6.9e-254
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG
Sbjct: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120
           TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA
Sbjct: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120

Query: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180
           RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA
Sbjct: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180

Query: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240
           KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD
Sbjct: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240

Query: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300
           YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA
Sbjct: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300

Query: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360
           AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG
Sbjct: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360

Query: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420
           ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK
Sbjct: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420

Query: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480
           DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY
Sbjct: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480

Query: 481 QTATRKEYRA 491
           QTATRKEYRA
Sbjct: 481 QTATRKEYRA 490

BLAST of Carg18475 vs. NCBI nr
Match: XP_022944102.1 (seed biotin-containing protein SBP65-like [Cucurbita moschata])

HSP 1 Score: 879.0 bits (2270), Expect = 1.9e-251
Identity = 487/490 (99.39%), Postives = 487/490 (99.39%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG
Sbjct: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120
           TSIAKGAL GREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRE DTENERERRA
Sbjct: 61  TSIAKGALTGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKREPDTENERERRA 120

Query: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180
           RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA
Sbjct: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180

Query: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240
           KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD
Sbjct: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240

Query: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300
           YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAK VAIGGGKVAAEYAGKA
Sbjct: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKDVAIGGGKVAAEYAGKA 300

Query: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360
           AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG
Sbjct: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360

Query: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420
           ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK
Sbjct: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420

Query: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480
           DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY
Sbjct: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480

Query: 481 QTATRKEYRA 491
           QTATRKEYRA
Sbjct: 481 QTATRKEYRA 490

BLAST of Carg18475 vs. NCBI nr
Match: KAG6570540.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 874.0 bits (2257), Expect = 6.0e-250
Identity = 486/490 (99.18%), Postives = 488/490 (99.59%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG
Sbjct: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120
           TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA
Sbjct: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120

Query: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180
           RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA
Sbjct: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180

Query: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240
           KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERG+QTGLGAVKETLTSAAKTAKD
Sbjct: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGKQTGLGAVKETLTSAAKTAKD 240

Query: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300
           YTVPIAEKAKDYTVQKAVEAKDITV VGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA
Sbjct: 241 YTVPIAEKAKDYTVQKAVEAKDITVSVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300

Query: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360
           AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG
Sbjct: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360

Query: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420
           ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEG+
Sbjct: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGR 420

Query: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480
            DDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY
Sbjct: 421 -DDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480

Query: 481 QTATRKEYRA 491
           QTATRKEYRA
Sbjct: 481 QTATRKEYRA 489

BLAST of Carg18475 vs. NCBI nr
Match: XP_023512588.1 (seed biotin-containing protein SBP65 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 837.0 bits (2161), Expect = 8.2e-239
Identity = 468/490 (95.51%), Postives = 477/490 (97.35%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MAAEQL+RRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG
Sbjct: 1   MAAEQLSRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120
           TSIAKGALPGREVRRS+AEGEVG GKPD+THELAAQFDSLAEKVRDKRETDTENERERRA
Sbjct: 61  TSIAKGALPGREVRRSVAEGEVGDGKPDETHELAAQFDSLAEKVRDKRETDTENERERRA 120

Query: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180
           RADKEREQRRSRIGEEQRL EQKKGKE E+G RQ QPSLEEISNVRATAQEKSNK TRAA
Sbjct: 121 RADKEREQRRSRIGEEQRLPEQKKGKEDEMGFRQNQPSLEEISNVRATAQEKSNKATRAA 180

Query: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240
           KERYDEAKKESLNQGVGARTEEWDQ GTEGAE TVEERGQQTGLGAVKETLTSAAKTAKD
Sbjct: 181 KERYDEAKKESLNQGVGARTEEWDQGGTEGAEGTVEERGQQTGLGAVKETLTSAAKTAKD 240

Query: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300
           YTVPIAEKAKDYTVQKAVEAKDITV VGQTTAHYLGEKAVAAK VAIGGGKVAAEYAGKA
Sbjct: 241 YTVPIAEKAKDYTVQKAVEAKDITVSVGQTTAHYLGEKAVAAKDVAIGGGKVAAEYAGKA 300

Query: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360
           AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAA+PLSAAKNVAESTG
Sbjct: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAAKPLSAAKNVAESTG 360

Query: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420
           ESVKEYTARKKEEAQRESTYKQAEKESMYEK++ETFE AREEVKERGEGRVNEKNAA G+
Sbjct: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKSRETFEWAREEVKERGEGRVNEKNAAGGR 420

Query: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480
            DDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEG+ANDQGYGREEHY
Sbjct: 421 -DDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGEANDQGYGREEHY 480

Query: 481 QTATRKEYRA 491
           QTATRKEYRA
Sbjct: 481 QTATRKEYRA 489

BLAST of Carg18475 vs. NCBI nr
Match: XP_022985959.1 (seed biotin-containing protein SBP65-like [Cucurbita maxima])

HSP 1 Score: 831.2 bits (2146), Expect = 4.5e-237
Identity = 465/490 (94.90%), Postives = 476/490 (97.14%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MAAEQL+RRDNTTKEREIQVEKE+VPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG
Sbjct: 1   MAAEQLSRRDNTTKEREIQVEKEKVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120
           TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENER+RRA
Sbjct: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERKRRA 120

Query: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180
           RADKEREQ+ SRIGEEQRLSEQKKGKEG+IGSRQ QPSLEEISNVRAT QEKSNK T AA
Sbjct: 121 RADKEREQKGSRIGEEQRLSEQKKGKEGDIGSRQNQPSLEEISNVRATEQEKSNKATMAA 180

Query: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240
           KERYDEAKKESLNQGVGARTEE DQRGTEGAEDTVEE+GQQTGLGAV+ETLTSAAKTAKD
Sbjct: 181 KERYDEAKKESLNQGVGARTEESDQRGTEGAEDTVEEKGQQTGLGAVEETLTSAAKTAKD 240

Query: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300
           YTVPIAEKAKDYTVQKAVEAKDITV VGQTTAHYLGEKAVAAK V IGGGKVAAEYAGKA
Sbjct: 241 YTVPIAEKAKDYTVQKAVEAKDITVTVGQTTAHYLGEKAVAAKDVVIGGGKVAAEYAGKA 300

Query: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360
            EDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAA+PLSAAK VAESTG
Sbjct: 301 TEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAAKPLSAAKTVAESTG 360

Query: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420
           ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEG+
Sbjct: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGR 420

Query: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480
            DDGGILGAIGETIYEIAQTTKEIVIGHGDETRK +TFDFEHGEEEG+ANDQGYGR EHY
Sbjct: 421 -DDGGILGAIGETIYEIAQTTKEIVIGHGDETRKQNTFDFEHGEEEGEANDQGYGRGEHY 480

Query: 481 QTATRKEYRA 491
           QTATRKEYRA
Sbjct: 481 QTATRKEYRA 489

BLAST of Carg18475 vs. ExPASy Swiss-Prot
Match: Q39846 (Seed biotin-containing protein SBP65 OS=Glycine max OX=3847 GN=SBP65 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.0e-46
Identity = 189/516 (36.63%), Postives = 267/516 (51.74%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MA+EQL RR+NTT E+EI VEK RVP++ +HFE +A Q K +         + G    QG
Sbjct: 1   MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESD-------ITAGKDTPQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGG-GKPDDTHELAAQFDSLAEKVRDKRETDT----ENE 120
           +  A  A    +     A G++GG GK  +THEL A F+SLA+KV D    +     E++
Sbjct: 61  SIEALQAGERVKDHAGKAMGDIGGRGKARETHELGAHFESLADKVTDHAAANVVGNKESQ 120

Query: 121 RERRA----------------RADKEREQRRSR--------------------IGEEQRL 180
           RE R                 + +K+R + ++R                    + E+ R 
Sbjct: 121 REARGGVRDVGKFEMRTEGGEKGNKDRPELKTRTREVIGRTEKERGRESGGQVVAEKGRE 180

Query: 181 SEQKKGKEG----------------EIGSRQYQPSLEE--ISNVRATAQEK----SNKVT 240
           +E  +G+ G                E G    +P  EE   S    +A E+    S++ T
Sbjct: 181 TETARGRVGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQISNYSDQAT 240

Query: 241 RAAKERYDEAKK---ESLNQGVGARTEEWDQRGTEGAE--DTVEERGQQTGLGAVKETLT 300
           +  KE+Y+ AK+   E+LN       E+  Q     A+  D   E+GQQ G    K+T++
Sbjct: 241 QGVKEKYERAKQAASETLNTTTQTAQEKSAQAKNLAAQAKDATLEKGQQ-GYAVTKDTIS 300

Query: 301 SAAKTAKDYTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKV 360
           SAAKTA + T P+AEKAKDYT+Q A +AK      G TTA Y+GEKAV AK VA+  GK 
Sbjct: 301 SAAKTASEKTAPVAEKAKDYTLQAAEKAKS----AGGTTASYVGEKAVQAKDVAVESGKS 360

Query: 361 AAEYAGKAAEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAG----AIAARP 420
           AA YA K A DL+DKA   GW+ AH+S +  VEGTKAAA +V+GAA  AG     +A+  
Sbjct: 361 AAGYAAKVAADLRDKATAVGWAAAHFSAEKTVEGTKAAAHVVEGAAGYAGHKAAELASMS 420

Query: 421 LSAAKNVAESTGESVKEYTARKKEEAQRESTYKQAEKESMYEKTKE-TFEGAREEVKERG 444
             A K +A S GE+ KEYTA+KKEEAQRE    +A+K S  ++ +E   EG  E V++  
Sbjct: 421 AGAVKGLAASAGETAKEYTAKKKEEAQRE---LEAKKPSQPQEAEERPSEGIGETVRQYA 480

BLAST of Carg18475 vs. ExPASy Swiss-Prot
Match: Q41060 (Seed biotin-containing protein SBP65 OS=Pisum sativum OX=3888 GN=SBP65 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.5e-30
Identity = 174/558 (31.18%), Postives = 253/558 (45.34%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASD------G 60
           MA+EQL+RR+N T ER+IQ  ++ VPQ T+HFE            +THEL  +       
Sbjct: 1   MASEQLSRRENITTERKIQNAEDSVPQRTTHFEL----------RETHELGPNFQSLPRN 60

Query: 61  GKEAQGTSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTEN 120
             +A     A+  L        +    V        H +  + D     VRD  +   E+
Sbjct: 61  ENQAYLDRGARAPLSANVSESYLDRARVPLNANIPEHRVREKED--FGGVRDMGKFQMES 120

Query: 121 ERERRARA-DKEREQRRSRI-----------GEEQRLSEQKKGKEGEIGSRQYQPSLEEI 180
           +   ++ A D+E    RSR+           G+ Q + E+++ +E  +   + + ++EEI
Sbjct: 121 KGGNKSLAEDRETLDTRSRMVTGTPHIKEASGKGQVVEERERARERAMEEEEKRLTMEEI 180

Query: 181 SNVRATAQEKSNKVTRAAKERYDEAKK---ESLNQGVGARTE------------EWDQRG 240
           S  R  AQ+ + +   AA+E+Y+ AK+   E+L     A  E            E  Q+G
Sbjct: 181 SKYRNQAQQSALEALSAAQEKYERAKQATNETLRNTTQAAQEKGEAAQAKDATFEKTQQG 240

Query: 241 TEGAEDTVEERGQ-----------------QTGLGAVKETLTSAAKTAKDYTVPIAEKAK 300
            E   DTV    +                 Q G  A ++T+++AA+TA +Y  P AEKA+
Sbjct: 241 YEMTGDTVSNSARTASEKAAQAKNTTLGKTQQGYEATRDTVSNAARTAAEYATPAAEKAR 300

Query: 301 DYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKAAEDLKDKAVV 360
                 AV+AKD+T+  G+T A    EKA           K AAE A K A DLK+KA V
Sbjct: 301 CV----AVQAKDVTLETGKTAA----EKA-----------KCAAEIAAKVAVDLKEKATV 360

Query: 361 AGWSTAHYSCDTAVEGTKAAARLVKGA----AEQAGAIAARPLSAAKNVAESTGESVKEY 420
           AGW+ +HY+    V+GT+AAA  V+GA    A +A  +AA+ +   K +A S GE+ KE+
Sbjct: 361 AGWTASHYATQLTVDGTRAAANAVEGAVGYVAPKASELAAKSVETVKGLAASAGETAKEF 420

Query: 421 TARKKEEAQRESTYK----------------------------QAEKESMYEKTK----- 455
           TARKKEE+ RE   K                            QA+  ++ EK +     
Sbjct: 421 TARKKEESWREYEAKRASQLQEGEEILPSTGGIGKVLPSGERTQAQGTNLQEKVQGKGSD 480

BLAST of Carg18475 vs. ExPASy Swiss-Prot
Match: Q9SKP0 (Late embryogenesis abundant protein ECP63 OS=Arabidopsis thaliana OX=3702 GN=ECP63 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 5.5e-04
Identity = 97/346 (28.03%), Postives = 137/346 (39.60%), Query Frame = 0

Query: 140 SEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAAKERYDEAKK---------- 199
           S+++K +  E+ +R     L +I N    A     KVT    E   E  +          
Sbjct: 3   SDKQKAERAEVAARLAAEDLHDI-NKSGGADVTMYKVTERTTEHPPEQDRPGVIGSVFRA 62

Query: 200 -----ESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKDYTVP 259
                E     V  +T E  +   EGA+   E+      +GA   T+  A +TA DYT  
Sbjct: 63  VQGTYEHARDAVVGKTHEAAESTKEGAQIASEK-----AVGAKDATVEKAKETA-DYTAE 122

Query: 260 IAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKAAEDL 319
              + KDYTV KA EAKD T    + TA+Y  +KAV AK           +YA   A + 
Sbjct: 123 KVGEYKDYTVDKAKEAKDTTAEKAKETANYTADKAVEAKDKTAEKIGEYKDYAVDKAVEA 182

Query: 320 KDKAVVAGWSTAHYSCDTAVEGTKAAAR--------LVKGAAEQAGAIAARPLSAAKNVA 379
           KDK       TA+Y+ D A E     A          V  A E     A + + A    A
Sbjct: 183 KDKTAEKAKETANYTADKAKEAKDKTAEKVGEYKDYTVDKAVEARDYTAEKAIEAKDKTA 242

Query: 380 ESTGESVKEYTARKKEEAQRESTYKQAE-KESMYEKTK------------------ETFE 439
           E TGE  K+YT  K  E +  +  K  E K+S  E  K                  ET +
Sbjct: 243 EKTGE-YKDYTVEKATEGKDVTVSKLGELKDSAVETAKRAMGFLSGKTEEAKGKAVETKD 302

Query: 440 GAREEVKERGEGRVNEKNAAEGKDDDGGILGAIGETIYEIAQTTKE 444
            A+E +++ GE  V  +   E + +   +    G    E +Q T+E
Sbjct: 303 TAKENMEKAGE--VTRQKMEEMRLEGKELKEEAGAKAQEASQKTRE 338

BLAST of Carg18475 vs. ExPASy TrEMBL
Match: A0A6J1FUV3 (seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC111448646 PE=4 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 9.1e-252
Identity = 487/490 (99.39%), Postives = 487/490 (99.39%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG
Sbjct: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120
           TSIAKGAL GREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRE DTENERERRA
Sbjct: 61  TSIAKGALTGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKREPDTENERERRA 120

Query: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180
           RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA
Sbjct: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180

Query: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240
           KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD
Sbjct: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240

Query: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300
           YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAK VAIGGGKVAAEYAGKA
Sbjct: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKDVAIGGGKVAAEYAGKA 300

Query: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360
           AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG
Sbjct: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360

Query: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420
           ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK
Sbjct: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420

Query: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480
           DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY
Sbjct: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480

Query: 481 QTATRKEYRA 491
           QTATRKEYRA
Sbjct: 481 QTATRKEYRA 490

BLAST of Carg18475 vs. ExPASy TrEMBL
Match: A0A6J1JER8 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111483844 PE=4 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.2e-237
Identity = 465/490 (94.90%), Postives = 476/490 (97.14%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60
           MAAEQL+RRDNTTKEREIQVEKE+VPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG
Sbjct: 1   MAAEQLSRRDNTTKEREIQVEKEKVPQLTSHFEAIAVQCKAATPEKTHELASDGGKEAQG 60

Query: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERERRA 120
           TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENER+RRA
Sbjct: 61  TSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENERKRRA 120

Query: 121 RADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAA 180
           RADKEREQ+ SRIGEEQRLSEQKKGKEG+IGSRQ QPSLEEISNVRAT QEKSNK T AA
Sbjct: 121 RADKEREQKGSRIGEEQRLSEQKKGKEGDIGSRQNQPSLEEISNVRATEQEKSNKATMAA 180

Query: 181 KERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKD 240
           KERYDEAKKESLNQGVGARTEE DQRGTEGAEDTVEE+GQQTGLGAV+ETLTSAAKTAKD
Sbjct: 181 KERYDEAKKESLNQGVGARTEESDQRGTEGAEDTVEEKGQQTGLGAVEETLTSAAKTAKD 240

Query: 241 YTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKA 300
           YTVPIAEKAKDYTVQKAVEAKDITV VGQTTAHYLGEKAVAAK V IGGGKVAAEYAGKA
Sbjct: 241 YTVPIAEKAKDYTVQKAVEAKDITVTVGQTTAHYLGEKAVAAKDVVIGGGKVAAEYAGKA 300

Query: 301 AEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNVAESTG 360
            EDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAA+PLSAAK VAESTG
Sbjct: 301 TEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAAKPLSAAKTVAESTG 360

Query: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGK 420
           ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEG+
Sbjct: 361 ESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKNAAEGR 420

Query: 421 DDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYGREEHY 480
            DDGGILGAIGETIYEIAQTTKEIVIGHGDETRK +TFDFEHGEEEG+ANDQGYGR EHY
Sbjct: 421 -DDGGILGAIGETIYEIAQTTKEIVIGHGDETRKQNTFDFEHGEEEGEANDQGYGRGEHY 480

Query: 481 QTATRKEYRA 491
           QTATRKEYRA
Sbjct: 481 QTATRKEYRA 489

BLAST of Carg18475 vs. ExPASy TrEMBL
Match: A0A5A7ULM4 (Seed biotin-containing protein SBP65 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G002010 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 5.1e-162
Identity = 361/494 (73.08%), Postives = 396/494 (80.16%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHEL----ASDGGK 60
           MA+EQL+RRDNTT EREIQVEKERVPQLTSHFEAIA Q KA  PEKT E     AS G K
Sbjct: 1   MASEQLSRRDNTTNEREIQVEKERVPQLTSHFEAIAGQGKATIPEKTEEKQRSGASGGRK 60

Query: 61  EAQGTSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENER 120
           E  G SIAKG LPGRE  R   EGE  GGKP++THELA+QF+SLA+KVRD RETDTENER
Sbjct: 61  EVHGISIAKGELPGREEERKRVEGE--GGKPNETHELASQFESLAQKVRDNRETDTENER 120

Query: 121 ERRARADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKV 180
           +R+   D+E EQR S+    QR SEQ K KEGEIG+RQ QPSL+EISN RA AQE+SN+ 
Sbjct: 121 QRQ---DRENEQRVSK----QRDSEQAKEKEGEIGTRQNQPSLDEISNYRAIAQERSNEA 180

Query: 181 TRAAKERYDEAKKESLNQGVGARTEEWDQRGT-EGAEDTVEERGQQTGLGAVKETLTSAA 240
            RAAKERY++A KESL+QGV  +TEE +QR T EG E+TVE  G++TGL  VKETLTSAA
Sbjct: 181 IRAAKERYEKANKESLHQGVEGKTEESEQRETEEGEENTVEVGGERTGLETVKETLTSAA 240

Query: 241 KTAKDYTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAE 300
           KTAKDYTVPIAEKAKDYTVQKA+EAKDITV  GQTTAHYLGEKAVAAK VA+  GKVAAE
Sbjct: 241 KTAKDYTVPIAEKAKDYTVQKAIEAKDITVSAGQTTAHYLGEKAVAAKDVAVESGKVAAE 300

Query: 301 YAGKAAEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNV 360
           YAGKAAEDLKDKAVVAGWSTAHYSCDTA+EGTKAAARLVKGAAE AGAIA +PLSAAKNV
Sbjct: 301 YAGKAAEDLKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAGKPLSAAKNV 360

Query: 361 AESTGESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKN 420
           A+STGESVKEYTARKKE A+RE+TYK  EKE+MYEKTK TFEG +EE+K RG    NEK 
Sbjct: 361 AQSTGESVKEYTARKKEVAEREATYKTGEKETMYEKTKGTFEGGKEELKGRG----NEKR 420

Query: 421 AAEGKDDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYG 480
                DDDGGILGAIGETIYEIAQTTKEIVIG GDE+ K S   FE GE           
Sbjct: 421 V----DDDGGILGAIGETIYEIAQTTKEIVIGDGDESGKQSKLGFEIGE----------- 465

Query: 481 REEHYQTATRKEYR 490
            EE Y+T TRKE R
Sbjct: 481 -EEQYETDTRKEQR 465

BLAST of Carg18475 vs. ExPASy TrEMBL
Match: A0A1S3C372 (seed biotin-containing protein SBP65 OS=Cucumis melo OX=3656 GN=LOC103496368 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 6.7e-162
Identity = 361/494 (73.08%), Postives = 396/494 (80.16%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHEL----ASDGGK 60
           MA+EQL+RRDNTT EREIQVEKERVPQLTSHFEAIA Q KA  PEKT E     AS G K
Sbjct: 1   MASEQLSRRDNTTNEREIQVEKERVPQLTSHFEAIAGQGKATIPEKTEEKQRSGASGGRK 60

Query: 61  EAQGTSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDTENER 120
           E  G SIAKG LPGRE  R   EGE  GGKP++THELA+QF+SLA+KVRD RETDTENER
Sbjct: 61  EVHGISIAKGELPGREEERKRVEGE--GGKPNETHELASQFESLAQKVRDNRETDTENER 120

Query: 121 ERRARADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKV 180
           +R+   D+E EQR S+    QR SEQ K KEGEIG+RQ QPSL+EISN RA AQE+SN+ 
Sbjct: 121 QRQ---DRENEQRVSK----QRDSEQAKEKEGEIGTRQNQPSLDEISNYRAIAQERSNEA 180

Query: 181 TRAAKERYDEAKKESLNQGVGARTEEWDQRGT-EGAEDTVEERGQQTGLGAVKETLTSAA 240
            RAAKERY++A KESL+QGV  +TEE +QR T EG E+TVE  G++TGL  VKETLTSAA
Sbjct: 181 IRAAKERYEKANKESLHQGVEGKTEESEQRETEEGEENTVEVGGERTGLETVKETLTSAA 240

Query: 241 KTAKDYTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAE 300
           KTAKDYTVPIAEKAKDYTVQKA+EAKDITV  GQTTAHYLGEKAVAAK VA+  GKVAAE
Sbjct: 241 KTAKDYTVPIAEKAKDYTVQKAIEAKDITVSAGQTTAHYLGEKAVAAKDVAVESGKVAAE 300

Query: 301 YAGKAAEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAAKNV 360
           YAGKAAEDLKDKAVVAGWSTAHYSCDTA+EGTKAAARLVKGAAE AGAIA +PLSAAKNV
Sbjct: 301 YAGKAAEDLKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAGKPLSAAKNV 360

Query: 361 AESTGESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVNEKN 420
           A+STGESVKEYTARKKE A+RE+TYK  EKE+MYEKTK TFEG +EE+K RG    NEK 
Sbjct: 361 AQSTGESVKEYTARKKEIAEREATYKTGEKETMYEKTKGTFEGGKEELKGRG----NEKR 420

Query: 421 AAEGKDDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGEEEGQANDQGYG 480
                DDDGGILGAIGETIYEIAQTTKEIVIG GDE+ K S   FE GE           
Sbjct: 421 V----DDDGGILGAIGETIYEIAQTTKEIVIGDGDESGKQSKLGFEIGE----------- 465

Query: 481 REEHYQTATRKEYR 490
            EE Y+T TRKE R
Sbjct: 481 -EEQYETDTRKEQR 465

BLAST of Carg18475 vs. ExPASy TrEMBL
Match: A0A6J1I6Z1 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111469726 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 7.6e-158
Identity = 356/508 (70.08%), Postives = 392/508 (77.17%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQC--------KAATPEKTHEL-- 60
           MA E+L+RRD+TTKE+EIQVE+ERVPQLTSHFEAIAV          KA TPE+T     
Sbjct: 1   MATEKLSRRDDTTKEKEIQVERERVPQLTSHFEAIAVHGKATDPSNEKATTPEETKARQK 60

Query: 61  --ASDGGKEAQGTSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKR 120
             AS  GKE  GT IA+G + GRE  R      VGG KP+D+HELAAQF+SLA+KVRDKR
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQ----GVGGEKPEDSHELAAQFESLADKVRDKR 120

Query: 121 ETDTENERERRARADK------EREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEI 180
           ETDTENERER+ARADK      EREQ+ SR  E+QR  EQ K +EGEIGSRQ QPSLEEI
Sbjct: 121 ETDTENERERQARADKERIAAREREQKGSRAEEKQRALEQAKEREGEIGSRQNQPSLEEI 180

Query: 181 SNVRATAQEKSNKVTRAAKERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQT 240
           SN RA AQ+KSN+   AAKERYD+A K+SL QGV A T E   RGTE AEDTVEE  Q +
Sbjct: 181 SNYRAIAQQKSNEAIMAAKERYDKANKKSLEQGVEAGTREAIPRGTEEAEDTVEEGNQYS 240

Query: 241 GLGAVKETLTSAAKTAKDYTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAA 300
           GL  VKETLTSAAKTAKDYT PIAEKAKDYTV             G+TTAHYLGEKAV A
Sbjct: 241 GLETVKETLTSAAKTAKDYTAPIAEKAKDYTVS-----------AGRTTAHYLGEKAVVA 300

Query: 301 KGVAIGGGKVAAEYAGKAAEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAG 360
           K VAI  GKVAAEYAGKAAEDLKDK  VAGWSTAHYSCDTAVEGTKAAARLVKGAAE AG
Sbjct: 301 KDVAIESGKVAAEYAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAG 360

Query: 361 AIAARPLSAAKNVAESTGESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREE 420
           AIAA+PLSAAKNVAESTGE+VK+YTARKKEEAQRES YKQ EKESMYEKTKETFE   EE
Sbjct: 361 AIAAKPLSAAKNVAESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEEGIEE 420

Query: 421 VKERGEGRVNEKNAAEGKDDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEH 480
           VKERG G   E+N  E   +DGGILGAIGETIYEIAQTTK+IVIG GDET K ST  FE 
Sbjct: 421 VKERG-GERGEENRVEEDRNDGGILGAIGETIYEIAQTTKDIVIGPGDETGKQSTLGFEL 480

Query: 481 GEEEGQANDQGYGREEHYQTATRKEYRA 491
           G+EE +   Q + ++EHY+TA +KE+RA
Sbjct: 481 GQEEDEEKGQEHEKKEHYETAAKKEHRA 492

BLAST of Carg18475 vs. TAIR 10
Match: AT2G42560.1 (late embryogenesis abundant domain-containing protein / LEA domain-containing protein )

HSP 1 Score: 101.3 bits (251), Expect = 2.3e-21
Identity = 190/656 (28.96%), Postives = 256/656 (39.02%), Query Frame = 0

Query: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQCKAATPEKTHELASDGG----- 60
           MA+EQ  RR+N   ERE+QVEK+RVP++TSHFE++A + K      TH   ++GG     
Sbjct: 1   MASEQA-RRENKVTEREVQVEKDRVPKMTSHFESMAEKGK---DSDTHRHQTEGGGTQFV 60

Query: 61  ------------KEAQGTSIAKGALPGREVRRSIAEGEVGGGKPDD---THELAAQFD-- 120
                        E +G +  K       V + +   + G GK  D    HE A+  D  
Sbjct: 61  SLSDKGSNMPVSDEGEGETKMKRTQMPHSVGKFVTSSDSGTGKKKDEKEEHEKASLEDIH 120

Query: 121 ----SLAEKVRDKRETDTENERERRARADKEREQRRSRIGEEQ---------RLS----- 180
               +  +K  D  +   E   + +       ++ R   GEE          R+S     
Sbjct: 121 GYRANAQQKSMDSIKAAEERYNKAKESLSHSGQEARGGRGEEMVGKGRDSGVRVSHVGAV 180

Query: 181 -------EQKKGKEGEIGSRQYQPSLEEISNVRATAQE----------KSNKVTRAAKER 240
                  E++ G  G  G +     L          +E          +S K T A K  
Sbjct: 181 GGGGGGEEKESGVHGFHGEKARHAELLAAGGEEMREREGKESAGGVGGRSVKDTVAEK-- 240

Query: 241 YDEAKKESLNQG---VGARTEEWDQRGTEGAEDTVEERGQQTGLGA-------------V 300
             +  KES+ +G    G+ T E  QR +E A +  +E G  T   A              
Sbjct: 241 -GQQAKESVGEGAQKAGSATSEKAQRASEYATEKGKEAGNMTAEQAARAKDYALQKAVEA 300

Query: 301 KETLTSAAKTAKDY-------TVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYL----- 360
           KET    A+ A +Y           A +AKDYT+QKAVEAKD+     Q  + Y+     
Sbjct: 301 KETAAEKAQRASEYMKETGSTAAEQAARAKDYTLQKAVEAKDVAAEKAQRASEYMTETGK 360

Query: 361 ----------------------------GEKAVAAKGVAIGGGKVAAEYAGKAAEDLKDK 420
                                       GE A   K   + GGK AA YAG AAE    K
Sbjct: 361 QAGNVAAQKGQEAASMTAKAKDYTVQKAGEAAGYIKETTVEGGKGAAHYAGVAAE----K 420

Query: 421 AVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAG----AIAARPLSAAKNVAESTGESV 480
           A   GW+ AH++ +  V+GTKA A  V+GA   AG     + ++ +   K  A    ++V
Sbjct: 421 AAAVGWTAAHFTTEKVVQGTKAVAGTVEGAVGYAGHKAVEVGSKAVDLTKEKAAVAADTV 480

Query: 481 KEYTARKKEEAQR----------------------------------------------- 482
             YTARKKEEAQ                                                
Sbjct: 481 VGYTARKKEEAQHRDQEMHQGGEEEKQPGFVSGARRDFGEEYGEERGSEKDVYGYGAKGI 540

BLAST of Carg18475 vs. TAIR 10
Match: AT2G36640.1 (embryonic cell protein 63 )

HSP 1 Score: 47.4 bits (111), Expect = 3.9e-05
Identity = 97/346 (28.03%), Postives = 137/346 (39.60%), Query Frame = 0

Query: 140 SEQKKGKEGEIGSRQYQPSLEEISNVRATAQEKSNKVTRAAKERYDEAKK---------- 199
           S+++K +  E+ +R     L +I N    A     KVT    E   E  +          
Sbjct: 3   SDKQKAERAEVAARLAAEDLHDI-NKSGGADVTMYKVTERTTEHPPEQDRPGVIGSVFRA 62

Query: 200 -----ESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLTSAAKTAKDYTVP 259
                E     V  +T E  +   EGA+   E+      +GA   T+  A +TA DYT  
Sbjct: 63  VQGTYEHARDAVVGKTHEAAESTKEGAQIASEK-----AVGAKDATVEKAKETA-DYTAE 122

Query: 260 IAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKGVAIGGGKVAAEYAGKAAEDL 319
              + KDYTV KA EAKD T    + TA+Y  +KAV AK           +YA   A + 
Sbjct: 123 KVGEYKDYTVDKAKEAKDTTAEKAKETANYTADKAVEAKDKTAEKIGEYKDYAVDKAVEA 182

Query: 320 KDKAVVAGWSTAHYSCDTAVEGTKAAAR--------LVKGAAEQAGAIAARPLSAAKNVA 379
           KDK       TA+Y+ D A E     A          V  A E     A + + A    A
Sbjct: 183 KDKTAEKAKETANYTADKAKEAKDKTAEKVGEYKDYTVDKAVEARDYTAEKAIEAKDKTA 242

Query: 380 ESTGESVKEYTARKKEEAQRESTYKQAE-KESMYEKTK------------------ETFE 439
           E TGE  K+YT  K  E +  +  K  E K+S  E  K                  ET +
Sbjct: 243 EKTGE-YKDYTVEKATEGKDVTVSKLGELKDSAVETAKRAMGFLSGKTEEAKGKAVETKD 302

Query: 440 GAREEVKERGEGRVNEKNAAEGKDDDGGILGAIGETIYEIAQTTKE 444
            A+E +++ GE  V  +   E + +   +    G    E +Q T+E
Sbjct: 303 TAKENMEKAGE--VTRQKMEEMRLEGKELKEEAGAKAQEASQKTRE 338