Carg18446 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg18446 vs. NCBI nr
Match: KAG7010366.1 (LanC-like protein GCL2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 898.3 bits (2320), Expect = 2.7e-257
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60
           MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR
Sbjct: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60

Query: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120
           DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR
Sbjct: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120

Query: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180
           RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG
Sbjct: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180

Query: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240
           QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG
Sbjct: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240

Query: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLRNRFPSGNYPSSEED 300
           GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLRNRFPSGNYPSSEED
Sbjct: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLRNRFPSGNYPSSEED 300

Query: 301 RGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICHGVSG 360
           RGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICHGVSG
Sbjct: 301 RGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICHGVSG 360

Query: 361 NSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGGMAHL 420
           NSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGGMAHL
Sbjct: 361 NSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGGMAHL 420

Query: 421 FLDMIEPSMAKFPGYDL 438
           FLDMIEPSMAKFPGYDL
Sbjct: 421 FLDMIEPSMAKFPGYDL 437

BLAST of Carg18446 vs. NCBI nr
Match: XP_022943721.1 (lanC-like protein GCL2 isoform X1 [Cucurbita moschata] >XP_022943722.1 lanC-like protein GCL2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 782.3 bits (2019), Expect = 2.1e-222
Identity = 392/441 (88.89%), Postives = 393/441 (89.12%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60
           MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR
Sbjct: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60

Query: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120
           DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST              
Sbjct: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST-------------- 120

Query: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180
                                      DVTFICGRAGVCAIG VAAKRAGDEQLLCYYL 
Sbjct: 121 ---------------------------DVTFICGRAGVCAIGTVAAKRAGDEQLLCYYLA 180

Query: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240
           QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTR IVEEIIQRGRALAKG
Sbjct: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRVIVEEIIQRGRALAKG 240

Query: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL----RNRFPSGNYPS 300
           GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL    RNRFPSGNYPS
Sbjct: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLRYMIRNRFPSGNYPS 300

Query: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360
           SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH
Sbjct: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360

Query: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 420
           GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG
Sbjct: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 400

Query: 421 MAHLFLDMIEPSMAKFPGYDL 438
           MA+LFLDMIEPSMAKFPGYDL
Sbjct: 421 MAYLFLDMIEPSMAKFPGYDL 400

BLAST of Carg18446 vs. NCBI nr
Match: XP_023512306.1 (lanC-like protein GCL2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512307.1 lanC-like protein GCL2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512308.1 lanC-like protein GCL2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 780.4 bits (2014), Expect = 8.1e-222
Identity = 390/441 (88.44%), Postives = 394/441 (89.34%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60
           MGDRCFPNSMPDFVPETAAAVESILS PYPII ERFKRAALDLKEAIVLETWGVTGQRVR
Sbjct: 1   MGDRCFPNSMPDFVPETAAAVESILSTPYPIILERFKRAALDLKEAIVLETWGVTGQRVR 60

Query: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120
           DFTLY+GALGTAFLLLKAHEVTSNHIDLSLCAQI+KACDQASSMST              
Sbjct: 61  DFTLYSGALGTAFLLLKAHEVTSNHIDLSLCAQILKACDQASSMST-------------- 120

Query: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180
                                      DVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG
Sbjct: 121 ---------------------------DVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180

Query: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240
           QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGT+ASVHTRAIVEEIIQRGRALAKG
Sbjct: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTIASVHTRAIVEEIIQRGRALAKG 240

Query: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL----RNRFPSGNYPS 300
           GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL    RNRFPSGNYPS
Sbjct: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLRYMIRNRFPSGNYPS 300

Query: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360
           SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH
Sbjct: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360

Query: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 420
           GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG
Sbjct: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 400

Query: 421 MAHLFLDMIEPSMAKFPGYDL 438
           MA+LFLDMIEPSMAKFPGYDL
Sbjct: 421 MAYLFLDMIEPSMAKFPGYDL 400

BLAST of Carg18446 vs. NCBI nr
Match: XP_022985978.1 (lanC-like protein GCL2 isoform X1 [Cucurbita maxima] >XP_022985979.1 lanC-like protein GCL2 isoform X1 [Cucurbita maxima] >XP_022985980.1 lanC-like protein GCL2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 773.9 bits (1997), Expect = 7.6e-220
Identity = 386/441 (87.53%), Postives = 393/441 (89.12%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60
           MGDRCFPNSMPDFVPETAAAVESILSMPYPIISER KRAALDLKEAIVLETWGVTGQRVR
Sbjct: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERLKRAALDLKEAIVLETWGVTGQRVR 60

Query: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120
           DFTLY+GALGTAFLLLKAHEVT+N IDLSLCAQIVKACDQASSMST              
Sbjct: 61  DFTLYSGALGTAFLLLKAHEVTANRIDLSLCAQIVKACDQASSMST-------------- 120

Query: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180
                                      DVTFICGRAGVCAIGAVAA+RAGDEQLLCYYLG
Sbjct: 121 ---------------------------DVTFICGRAGVCAIGAVAARRAGDEQLLCYYLG 180

Query: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240
           QFNEIKLPRNLPDELLYGKVGFLWACL+LNKHIGEGTVASVHTRAIVEEIIQRGRALAKG
Sbjct: 181 QFNEIKLPRNLPDELLYGKVGFLWACLFLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240

Query: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGT----LRNRFPSGNYPS 300
           GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGT    +RNRFP+GNYPS
Sbjct: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTIRYMIRNRFPNGNYPS 300

Query: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360
           SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAA DAGEVVWRRGLLKRVGICH
Sbjct: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAADAGEVVWRRGLLKRVGICH 360

Query: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 420
           GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG
Sbjct: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 400

Query: 421 MAHLFLDMIEPSMAKFPGYDL 438
           MA+LFLDMIEPSMAKFPGYDL
Sbjct: 421 MAYLFLDMIEPSMAKFPGYDL 400

BLAST of Carg18446 vs. NCBI nr
Match: XP_022140440.1 (lanC-like protein GCL2 [Momordica charantia])

HSP 1 Score: 719.9 bits (1857), Expect = 1.3e-203
Identity = 358/448 (79.91%), Postives = 382/448 (85.27%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESI-------LSMPYPIISERFKRAALDLKEAIVLETWG 60
           MGDR FPNSMPDFVPETAAAVES        LSMPYPIISERFKRAALDLKEAIVLETWG
Sbjct: 1   MGDRFFPNSMPDFVPETAAAVESSGDSFLKDLSMPYPIISERFKRAALDLKEAIVLETWG 60

Query: 61  VTGQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLS 120
           VTGQRVRDFTLY+GALGTA LLLKAHEVTSNHIDLSLCAQIVKACDQASSMST       
Sbjct: 61  VTGQRVRDFTLYSGALGTALLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST------- 120

Query: 121 LEGVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQ 180
                                             DVTFICGRAG+CAIGAVAAKRAGDEQ
Sbjct: 121 ----------------------------------DVTFICGRAGICAIGAVAAKRAGDEQ 180

Query: 181 LLCYYLGQFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQR 240
           LL YYLG+FNEIKLP NLPDELLYG+VGFLWACL+LNK+IG GT+ASVHTRA+VEE+I+R
Sbjct: 181 LLSYYLGEFNEIKLPSNLPDELLYGRVGFLWACLFLNKYIGAGTIASVHTRAVVEEVIRR 240

Query: 241 GRALAKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLR----NRF 300
           GRALAK G SPLMFEWYGERYWG AHGLAGILH+LMDMELKPDEI+DVKGT+R    NRF
Sbjct: 241 GRALAKRGGSPLMFEWYGERYWGAAHGLAGILHVLMDMELKPDEIEDVKGTIRYMIQNRF 300

Query: 301 PSGNYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLL 360
           PSGNYPSSEEDR RDILVHWCHGAPG+ALTLVKAA+VFGE+EFV+AAV+AGEVVWRRGLL
Sbjct: 301 PSGNYPSSEEDRNRDILVHWCHGAPGIALTLVKAAQVFGEKEFVEAAVEAGEVVWRRGLL 360

Query: 361 KRVGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYS 420
           KRVGICHG+SGNSYVFLSLYQLT KVEYLY+ KAFACFLLDRAHRLIAEGEMHGGDSR S
Sbjct: 361 KRVGICHGISGNSYVFLSLYQLTRKVEYLYRTKAFACFLLDRAHRLIAEGEMHGGDSRQS 407

Query: 421 LFEGVGGMAHLFLDMIEPSMAKFPGYDL 438
           LFEG+GGMA+LFLDMI P+MA+FPGYDL
Sbjct: 421 LFEGLGGMAYLFLDMIVPAMARFPGYDL 407

BLAST of Carg18446 vs. ExPASy Swiss-Prot
Match: Q8VZQ6 (LanC-like protein GCL2 OS=Arabidopsis thaliana OX=3702 GN=GCL2 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 5.6e-157
Identity = 267/446 (59.87%), Postives = 336/446 (75.34%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAA-----AVESILSMPYPIISERFKRAALDLKEAIVLETWGVT 60
           M  R F N MPDFV E  +      + ++L+MPY  +S++ KR+ALDLKE +V+ETWG +
Sbjct: 1   MAGRFFDNVMPDFVKEKESVSGGDTLRNLLAMPYSSLSQQLKRSALDLKETVVIETWGFS 60

Query: 61  GQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLE 120
           GQ V DFTLY+G LG AFLL +A++VT N  DLSLC +IVKACD AS+ S          
Sbjct: 61  GQTVEDFTLYSGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDTASASS---------- 120

Query: 121 GVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLL 180
                                           DVTF+CGRAGVC +GAVAAK +G+E LL
Sbjct: 121 -------------------------------GDVTFLCGRAGVCGLGAVAAKLSGEEDLL 180

Query: 181 CYYLGQFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGR 240
            YYLGQF  I+L  +LP+ELLYG+VG+LWACL++NK+IG+ T++S   R + +EII+ GR
Sbjct: 181 NYYLGQFRLIRLSSDLPNELLYGRVGYLWACLFINKYIGKETLSSDTIREVAQEIIKEGR 240

Query: 241 ALAKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL----RNRFPS 300
           ++AK G+SPLMFEWYG+RYWG AHGLAGI+H+LMD++LKPDE +DVKGTL    +NRFPS
Sbjct: 241 SMAKKGSSPLMFEWYGKRYWGAAHGLAGIMHVLMDVQLKPDEAEDVKGTLKYMIKNRFPS 300

Query: 301 GNYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKR 360
           GNYP+SEED+ +DILVHWCHGAPG+ALTL KAA+VFGE EF++A+  A EVVW RGLLKR
Sbjct: 301 GNYPASEEDKKKDILVHWCHGAPGIALTLGKAAEVFGEREFLEASAAAAEVVWNRGLLKR 360

Query: 361 VGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLF 420
           VGICHG+SGN+YVFL+LY+ TG+ EYLY+AKAFA FLLDR  +L+++GEMHGGDS YSLF
Sbjct: 361 VGICHGISGNAYVFLALYRATGRSEYLYRAKAFASFLLDRGPKLLSKGEMHGGDSPYSLF 405

Query: 421 EGVGGMAHLFLDMIEPSMAKFPGYDL 438
           EGV GMA+LFLDM++PS A+FPGY+L
Sbjct: 421 EGVAGMAYLFLDMVDPSEARFPGYEL 405

BLAST of Carg18446 vs. ExPASy Swiss-Prot
Match: F4IEM5 (LanC-like protein GCR2 OS=Arabidopsis thaliana OX=3702 GN=GCR2 PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 6.2e-140
Identity = 249/452 (55.09%), Postives = 317/452 (70.13%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVE-----------SILSMPYPIISERFKRAALDLKEAIVL 60
           MG+R F N MP+FVPE  +  E            +LS+PY   SE+  R AL +K+ +V 
Sbjct: 1   MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 60

Query: 61  ETWGVTGQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRA 120
           ETW  +G+RVRD+ LY G LGTA+LL K+++VT N  DL LC + V+ACD AS       
Sbjct: 61  ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVAS------- 120

Query: 121 RRLSLEGVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRA 180
                                          R+  +   VTFICG AGVCA+GAVAAK  
Sbjct: 121 -------------------------------RDSER---VTFICGYAGVCALGAVAAKCL 180

Query: 181 GDEQLLCYYLGQFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEE 240
           GD+QL   YL +F  I+LP +LP ELLYG+ G+LWACL+LNKHIG+ +++S   R++VEE
Sbjct: 181 GDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLNKHIGQESISSERMRSVVEE 240

Query: 241 IIQRGRALAKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL---- 300
           I + GR L   G  PLM+EW+G+RYWG AHGLAGI+++LM  EL+PDEI+DVKGTL    
Sbjct: 241 IFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMHTELEPDEIKDVKGTLSYMI 300

Query: 301 RNRFPSGNYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWR 360
           +NRFPSGNY SSE  +  D LVHWCHGAPGVALTLVKAA+V+  +EFV+AA++AGEVVW 
Sbjct: 301 QNRFPSGNYLSSEGSKS-DRLVHWCHGAPGVALTLVKAAQVYNTKEFVEAAMEAGEVVWS 360

Query: 361 RGLLKRVGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGD 420
           RGLLKRVGICHG+SGN+YVFLSLY+LT   +YLY+AKAFA FLLD++ +LI+EG+MHGGD
Sbjct: 361 RGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHGGD 410

Query: 421 SRYSLFEGVGGMAHLFLDMIEPSMAKFPGYDL 438
             +SLFEG+GGMA++ LDM +P+ A FPGY+L
Sbjct: 421 RPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410

BLAST of Carg18446 vs. ExPASy Swiss-Prot
Match: Q9FJN7 (LanC-like protein GCL1 OS=Arabidopsis thaliana OX=3702 GN=GCL1 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 6.9e-83
Identity = 186/446 (41.70%), Postives = 253/446 (56.73%), Query Frame = 0

Query: 21  VESILSMP-YPIIS---ERFKRAALDLKEAIVLETW--GVTGQR-----VRDFTLYAGAL 80
           ++ +LS P  P IS   E F RAA  LK  +V  TW  GV         V D T+Y G L
Sbjct: 30  IDHLLSEPSAPTISLPTESFLRAATLLKNQVVEATWKGGVEALASGSGPVLDPTVYTGLL 89

Query: 81  GTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMRRCASKDAGS 140
           GTAF  LK++EVT NH DL  CA+I+  C   +  +T                       
Sbjct: 90  GTAFTCLKSYEVTRNHQDLLTCAEIIDTCANVARATTRH--------------------- 149

Query: 141 QRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLGQFNEIKLPR 200
                              VTF+CGR GVC +GA+ A   GD+    ++LG F E+   R
Sbjct: 150 -------------------VTFLCGRGGVCTLGAIVANYRGDQSKRDFFLGLFLELAEER 209

Query: 201 NLPD-----------ELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGR-AL 260
            LP            +LLYG+ GFLWA L+LN+++G+GTV       IV  I+  GR   
Sbjct: 210 ELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQGTVPDHLLSPIVAAILAGGRVGA 269

Query: 261 AKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLR----NRFP-SG 320
           A   A PL++ ++G R+WG A+GLAGIL++L+   L  ++++DV+GTLR    NRFP SG
Sbjct: 270 ADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLSEEDVKDVQGTLRYMMSNRFPNSG 329

Query: 321 NYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEE-EFVQAAVDAGEVVWRRGLLKR 380
           NYP S E   RD LV W HGA G+A+TL KA++VF +E +F +AA++AGEVVW+ GL+K+
Sbjct: 330 NYPCS-EGNPRDKLVQWAHGATGMAITLAKASQVFPKERDFREAAIEAGEVVWKSGLVKK 389

Query: 381 VGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLF 438
           VG+  GV+GN+Y FLSLY+LTG V Y  +AKAFA +L   A  L+        +  YSLF
Sbjct: 390 VGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLCRDAIELVNMTSQE-TEHDYSLF 433

BLAST of Carg18446 vs. ExPASy Swiss-Prot
Match: Q9JJK2 (LanC-like protein 2 OS=Mus musculus OX=10090 GN=Lancl2 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 6.1e-63
Identity = 163/478 (34.10%), Postives = 235/478 (49.16%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAA-------AVESILSMPYP--------------IISERFKRA 60
           M +R FPN  PD+    +A       A E+    P P              I+    KR 
Sbjct: 18  MEERSFPNPFPDYEAAASAAGLAAGSAEETGRVCPLPTTEDPGLPFHPNGKIVPNFIKRI 77

Query: 61  ALDLKEAIVLETWGVTGQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACD 120
              +K+ +     G+      D + Y G  G A L L+ + VT                D
Sbjct: 78  QTKIKDLLQQMEEGLKTADPHDCSAYTGWTGIALLYLQLYRVTG---------------D 137

Query: 121 QASSMSTIRARRLSLEGVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVC 180
           Q   + ++   + +L  +  RR                           VTF+CG AG  
Sbjct: 138 QTYLLRSLDYVKRTLRNLSGRR---------------------------VTFLCGDAGPL 197

Query: 181 AIGAVAAKRAGDE----QLLCYYLGQFNEIKLPRN-LPDELLYGKVGFLWACLYLNKHIG 240
           A+GAV   +   E    + +   L     I    + LPDELLYG+ G+L+A LYLN  IG
Sbjct: 198 AVGAVIYHKLKSECESQECITKLLQMHRTIVCQESELPDELLYGRAGYLYALLYLNTEIG 257

Query: 241 EGTVASVHTRAIVEEIIQRGRALA----KGGASPLMFEWYGERYWGGAHGLAGILHILMD 300
            GTV     + +V  II+ G++L+    K    PL+++W+ ++Y G AHG+AGI ++LM 
Sbjct: 258 PGTVGETAIKEVVSAIIESGKSLSREERKSERCPLLYQWHRKQYVGAAHGMAGIYYMLMQ 317

Query: 301 MELKPDE---IQDVKGTL----RNRFPSGNYPSSEEDRGRDILVHWCHGAPGVALTLVKA 360
            E K D+    + VK ++      +F SGNYPSS  +   D LVHWCHGAPGV   L++A
Sbjct: 318 PEAKVDQETLTEMVKPSIDYVRHKKFRSGNYPSSLSNE-TDRLVHWCHGAPGVIHVLLQA 377

Query: 361 AKVFGEEEFVQAAVDAGEVVWRRGLLKR-VGICHGVSGNSYVFLSLYQLTGKVEYLYKAK 420
            +VF EE++++ A++  +V+W+RGLL++  GICHG SGN Y FLSLY+LT   +YLY+A 
Sbjct: 378 YQVFKEEKYLKEAMECSDVIWQRGLLRKGYGICHGTSGNGYSFLSLYRLTQDKKYLYRAC 437

Query: 421 AFACFLLDRAHRLIAEGEMHG---GDSRYSLFEGVGGMAHLFLDMIEPSMAKFPGYDL 438
            FA + LD           HG    D  YSLFEG+ G  H   D++ P  A+FP ++L
Sbjct: 438 KFAEWCLDYG--------AHGCRIPDRPYSLFEGMAGAVHFLSDILVPETARFPAFEL 444

BLAST of Carg18446 vs. ExPASy Swiss-Prot
Match: O89112 (Glutathione S-transferase LANCL1 OS=Mus musculus OX=10090 GN=Lancl1 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 7.5e-61
Identity = 122/302 (40.40%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 149 VTFICGRAGVCAIGAVAAKRAGDEQLLCYYLGQFNEI-KLPRNLPDELLYGKVGFLWACL 208
           +TF+CG AG  A+ AV   +   E+     + +   + K+  ++P+E+LYG++G+++A L
Sbjct: 104 ITFLCGDAGPLAVAAVLYHKMNSEKQAEECITRLIHLNKIDPHVPNEMLYGRIGYIFALL 163

Query: 209 YLNKHIGEGTVASVHTRAIVEEIIQRGRALAK----GGASPLMFEWYGERYWGGAHGLAG 268
           ++NK+ GE  +   H + I E I+  G  L++       SPLM+EWY E Y G AHGLAG
Sbjct: 164 FVNKNFGEEKIPQSHIQQICENILTSGENLSRKRNLAAKSPLMYEWYQEYYVGAAHGLAG 223

Query: 269 ILHILMDMELKPDEIQ-------DVKGTLRNRFPSGNYPSSEEDRGRDILVHWCHGAPGV 328
           I + LM   L+ ++ +        V    R +FPSGNYP   +D  RD+LVHWCHGAPGV
Sbjct: 224 IYYYLMQPSLQVNQGKLHSLVKPSVDFVCRLKFPSGNYPPCLDDT-RDLLVHWCHGAPGV 283

Query: 329 ALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKR-VGICHGVSGNSYVFLSLYQLTGKV 388
              L++A KVF EE ++  A    +V+W+ GLLK+  G+CHG +GN+Y FL+LY LT  +
Sbjct: 284 IYMLIQAYKVFKEERYLCDAQQCADVIWQYGLLKKGYGLCHGAAGNAYAFLALYNLTQDL 343

Query: 389 EYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGGMAHLFLDMIEPSMAKFPGY 438
           +YLY+A  FA + LD       E      D+ +SLFEG+ G  +   D++ P+ AKFP +
Sbjct: 344 KYLYRACKFAEWCLD-----YGEHGCRTADTPFSLFEGMAGTIYFLADLLVPTKAKFPAF 399

BLAST of Carg18446 vs. ExPASy TrEMBL
Match: A0A6J1FSH6 (lanC-like protein GCL2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448386 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.0e-222
Identity = 392/441 (88.89%), Postives = 393/441 (89.12%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60
           MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR
Sbjct: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60

Query: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120
           DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST              
Sbjct: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST-------------- 120

Query: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180
                                      DVTFICGRAGVCAIG VAAKRAGDEQLLCYYL 
Sbjct: 121 ---------------------------DVTFICGRAGVCAIGTVAAKRAGDEQLLCYYLA 180

Query: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240
           QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTR IVEEIIQRGRALAKG
Sbjct: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRVIVEEIIQRGRALAKG 240

Query: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL----RNRFPSGNYPS 300
           GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL    RNRFPSGNYPS
Sbjct: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLRYMIRNRFPSGNYPS 300

Query: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360
           SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH
Sbjct: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360

Query: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 420
           GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG
Sbjct: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 400

Query: 421 MAHLFLDMIEPSMAKFPGYDL 438
           MA+LFLDMIEPSMAKFPGYDL
Sbjct: 421 MAYLFLDMIEPSMAKFPGYDL 400

BLAST of Carg18446 vs. ExPASy TrEMBL
Match: A0A6J1JCS6 (lanC-like protein GCL2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483856 PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 3.7e-220
Identity = 386/441 (87.53%), Postives = 393/441 (89.12%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60
           MGDRCFPNSMPDFVPETAAAVESILSMPYPIISER KRAALDLKEAIVLETWGVTGQRVR
Sbjct: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERLKRAALDLKEAIVLETWGVTGQRVR 60

Query: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120
           DFTLY+GALGTAFLLLKAHEVT+N IDLSLCAQIVKACDQASSMST              
Sbjct: 61  DFTLYSGALGTAFLLLKAHEVTANRIDLSLCAQIVKACDQASSMST-------------- 120

Query: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180
                                      DVTFICGRAGVCAIGAVAA+RAGDEQLLCYYLG
Sbjct: 121 ---------------------------DVTFICGRAGVCAIGAVAARRAGDEQLLCYYLG 180

Query: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240
           QFNEIKLPRNLPDELLYGKVGFLWACL+LNKHIGEGTVASVHTRAIVEEIIQRGRALAKG
Sbjct: 181 QFNEIKLPRNLPDELLYGKVGFLWACLFLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240

Query: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGT----LRNRFPSGNYPS 300
           GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGT    +RNRFP+GNYPS
Sbjct: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTIRYMIRNRFPNGNYPS 300

Query: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360
           SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAA DAGEVVWRRGLLKRVGICH
Sbjct: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAADAGEVVWRRGLLKRVGICH 360

Query: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 420
           GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG
Sbjct: 361 GVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLFEGVGG 400

Query: 421 MAHLFLDMIEPSMAKFPGYDL 438
           MA+LFLDMIEPSMAKFPGYDL
Sbjct: 421 MAYLFLDMIEPSMAKFPGYDL 400

BLAST of Carg18446 vs. ExPASy TrEMBL
Match: A0A6J1CF36 (lanC-like protein GCL2 OS=Momordica charantia OX=3673 GN=LOC111011116 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.3e-204
Identity = 358/448 (79.91%), Postives = 382/448 (85.27%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESI-------LSMPYPIISERFKRAALDLKEAIVLETWG 60
           MGDR FPNSMPDFVPETAAAVES        LSMPYPIISERFKRAALDLKEAIVLETWG
Sbjct: 1   MGDRFFPNSMPDFVPETAAAVESSGDSFLKDLSMPYPIISERFKRAALDLKEAIVLETWG 60

Query: 61  VTGQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLS 120
           VTGQRVRDFTLY+GALGTA LLLKAHEVTSNHIDLSLCAQIVKACDQASSMST       
Sbjct: 61  VTGQRVRDFTLYSGALGTALLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST------- 120

Query: 121 LEGVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQ 180
                                             DVTFICGRAG+CAIGAVAAKRAGDEQ
Sbjct: 121 ----------------------------------DVTFICGRAGICAIGAVAAKRAGDEQ 180

Query: 181 LLCYYLGQFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQR 240
           LL YYLG+FNEIKLP NLPDELLYG+VGFLWACL+LNK+IG GT+ASVHTRA+VEE+I+R
Sbjct: 181 LLSYYLGEFNEIKLPSNLPDELLYGRVGFLWACLFLNKYIGAGTIASVHTRAVVEEVIRR 240

Query: 241 GRALAKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLR----NRF 300
           GRALAK G SPLMFEWYGERYWG AHGLAGILH+LMDMELKPDEI+DVKGT+R    NRF
Sbjct: 241 GRALAKRGGSPLMFEWYGERYWGAAHGLAGILHVLMDMELKPDEIEDVKGTIRYMIQNRF 300

Query: 301 PSGNYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLL 360
           PSGNYPSSEEDR RDILVHWCHGAPG+ALTLVKAA+VFGE+EFV+AAV+AGEVVWRRGLL
Sbjct: 301 PSGNYPSSEEDRNRDILVHWCHGAPGIALTLVKAAQVFGEKEFVEAAVEAGEVVWRRGLL 360

Query: 361 KRVGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYS 420
           KRVGICHG+SGNSYVFLSLYQLT KVEYLY+ KAFACFLLDRAHRLIAEGEMHGGDSR S
Sbjct: 361 KRVGICHGISGNSYVFLSLYQLTRKVEYLYRTKAFACFLLDRAHRLIAEGEMHGGDSRQS 407

Query: 421 LFEGVGGMAHLFLDMIEPSMAKFPGYDL 438
           LFEG+GGMA+LFLDMI P+MA+FPGYDL
Sbjct: 421 LFEGLGGMAYLFLDMIVPAMARFPGYDL 407

BLAST of Carg18446 vs. ExPASy TrEMBL
Match: A0A6J1FXB1 (lanC-like protein GCL2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448386 PE=3 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 1.8e-174
Identity = 314/363 (86.50%), Postives = 314/363 (86.50%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60
           MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR
Sbjct: 1   MGDRCFPNSMPDFVPETAAAVESILSMPYPIISERFKRAALDLKEAIVLETWGVTGQRVR 60

Query: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMR 120
           DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST              
Sbjct: 61  DFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMST-------------- 120

Query: 121 RCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLG 180
                                      DVTFICGRAGVCAIG VAAKRAGDEQLLCYYL 
Sbjct: 121 ---------------------------DVTFICGRAGVCAIGTVAAKRAGDEQLLCYYLA 180

Query: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGRALAKG 240
           QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTR IVEEIIQRGRALAKG
Sbjct: 181 QFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRVIVEEIIQRGRALAKG 240

Query: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL----RNRFPSGNYPS 300
           GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL    RNRFPSGNYPS
Sbjct: 241 GASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLRYMIRNRFPSGNYPS 300

Query: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 360
           SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH
Sbjct: 301 SEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKRVGICH 322

BLAST of Carg18446 vs. ExPASy TrEMBL
Match: A0A2P5EQK9 (LanC-like protein OS=Trema orientale OX=63057 GN=TorRG33x02_163780 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.7e-172
Identity = 300/457 (65.65%), Postives = 348/457 (76.15%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVES----------------ILSMPYPIISERFKRAALDLK 60
           M DR FPN MPDF+ ET A+  S                +LS PYP++S+  KRAALDLK
Sbjct: 1   MADRFFPNVMPDFIAETPASSPSYESEPEAGPGTDSLMRLLSTPYPVLSQHVKRAALDLK 60

Query: 61  EAIVLETWGVTGQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSM 120
           E IV+ETWGVTGQRV DFTLYAG LGTAFLL KA++VT+N  DL+LC+QIVKACD AS  
Sbjct: 61  ETIVIETWGVTGQRVHDFTLYAGTLGTAFLLFKAYQVTNNTNDLTLCSQIVKACDSAS-- 120

Query: 121 STIRARRLSLEGVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAV 180
                                  GS+                 DVTFICGR GVCA+GAV
Sbjct: 121 ----------------------LGSR-----------------DVTFICGRGGVCALGAV 180

Query: 181 AAKRAGDEQLLCYYLGQFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTR 240
           AAK AGDE+ L YYL QF EIKLP+NLPDELLYG+VG+LWACL+LNKH+GE  + S  TR
Sbjct: 181 AAKYAGDERALSYYLAQFKEIKLPKNLPDELLYGRVGYLWACLFLNKHLGEEMIPSSITR 240

Query: 241 AIVEEIIQRGRALAKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGT 300
            +V EII+ GRAL K    PLMFEWYGERYWG AHGLAGI+H+LMDMELKPDE++DVKGT
Sbjct: 241 PVVSEIIKNGRALGKKEKCPLMFEWYGERYWGAAHGLAGIMHVLMDMELKPDEVEDVKGT 300

Query: 301 LR----NRFPSGNYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAG 360
           LR    N FPSGNYP+SEED+GRD+LVHWCHGAPG+ALTLVKAAK+F  +EF++AA+DA 
Sbjct: 301 LRYMISNHFPSGNYPASEEDKGRDVLVHWCHGAPGIALTLVKAAKIFSAQEFLKAAIDAS 360

Query: 361 EVVWRRGLLKRVGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGE 420
           EVVW RGLLKRVGICHG+SGN+YVFLSLYQLTG VE+LY+AKAF+CFLLDRAH+L+++GE
Sbjct: 361 EVVWNRGLLKRVGICHGISGNAYVFLSLYQLTGNVEFLYRAKAFSCFLLDRAHKLMSQGE 416

Query: 421 MHGGDSRYSLFEGVGGMAHLFLDMIEPSMAKFPGYDL 438
           MHGGDS YSLFEG+GGMA+LFLDM+EP  AKFP YDL
Sbjct: 421 MHGGDSHYSLFEGIGGMAYLFLDMVEPFEAKFPAYDL 416

BLAST of Carg18446 vs. TAIR 10
Match: AT2G20770.1 (GCR2-like 2 )

HSP 1 Score: 555.4 bits (1430), Expect = 3.9e-158
Identity = 267/446 (59.87%), Postives = 336/446 (75.34%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAA-----AVESILSMPYPIISERFKRAALDLKEAIVLETWGVT 60
           M  R F N MPDFV E  +      + ++L+MPY  +S++ KR+ALDLKE +V+ETWG +
Sbjct: 1   MAGRFFDNVMPDFVKEKESVSGGDTLRNLLAMPYSSLSQQLKRSALDLKETVVIETWGFS 60

Query: 61  GQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLE 120
           GQ V DFTLY+G LG AFLL +A++VT N  DLSLC +IVKACD AS+ S          
Sbjct: 61  GQTVEDFTLYSGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDTASASS---------- 120

Query: 121 GVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLL 180
                                           DVTF+CGRAGVC +GAVAAK +G+E LL
Sbjct: 121 -------------------------------GDVTFLCGRAGVCGLGAVAAKLSGEEDLL 180

Query: 181 CYYLGQFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGR 240
            YYLGQF  I+L  +LP+ELLYG+VG+LWACL++NK+IG+ T++S   R + +EII+ GR
Sbjct: 181 NYYLGQFRLIRLSSDLPNELLYGRVGYLWACLFINKYIGKETLSSDTIREVAQEIIKEGR 240

Query: 241 ALAKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL----RNRFPS 300
           ++AK G+SPLMFEWYG+RYWG AHGLAGI+H+LMD++LKPDE +DVKGTL    +NRFPS
Sbjct: 241 SMAKKGSSPLMFEWYGKRYWGAAHGLAGIMHVLMDVQLKPDEAEDVKGTLKYMIKNRFPS 300

Query: 301 GNYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWRRGLLKR 360
           GNYP+SEED+ +DILVHWCHGAPG+ALTL KAA+VFGE EF++A+  A EVVW RGLLKR
Sbjct: 301 GNYPASEEDKKKDILVHWCHGAPGIALTLGKAAEVFGEREFLEASAAAAEVVWNRGLLKR 360

Query: 361 VGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLF 420
           VGICHG+SGN+YVFL+LY+ TG+ EYLY+AKAFA FLLDR  +L+++GEMHGGDS YSLF
Sbjct: 361 VGICHGISGNAYVFLALYRATGRSEYLYRAKAFASFLLDRGPKLLSKGEMHGGDSPYSLF 405

Query: 421 EGVGGMAHLFLDMIEPSMAKFPGYDL 438
           EGV GMA+LFLDM++PS A+FPGY+L
Sbjct: 421 EGVAGMAYLFLDMVDPSEARFPGYEL 405

BLAST of Carg18446 vs. TAIR 10
Match: AT1G52920.1 (G protein coupled receptor )

HSP 1 Score: 498.8 bits (1283), Expect = 4.4e-141
Identity = 249/452 (55.09%), Postives = 317/452 (70.13%), Query Frame = 0

Query: 1   MGDRCFPNSMPDFVPETAAAVE-----------SILSMPYPIISERFKRAALDLKEAIVL 60
           MG+R F N MP+FVPE  +  E            +LS+PY   SE+  R AL +K+ +V 
Sbjct: 1   MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 60

Query: 61  ETWGVTGQRVRDFTLYAGALGTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRA 120
           ETW  +G+RVRD+ LY G LGTA+LL K+++VT N  DL LC + V+ACD AS       
Sbjct: 61  ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVAS------- 120

Query: 121 RRLSLEGVDMRRCASKDAGSQRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRA 180
                                          R+  +   VTFICG AGVCA+GAVAAK  
Sbjct: 121 -------------------------------RDSER---VTFICGYAGVCALGAVAAKCL 180

Query: 181 GDEQLLCYYLGQFNEIKLPRNLPDELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEE 240
           GD+QL   YL +F  I+LP +LP ELLYG+ G+LWACL+LNKHIG+ +++S   R++VEE
Sbjct: 181 GDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLNKHIGQESISSERMRSVVEE 240

Query: 241 IIQRGRALAKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTL---- 300
           I + GR L   G  PLM+EW+G+RYWG AHGLAGI+++LM  EL+PDEI+DVKGTL    
Sbjct: 241 IFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMHTELEPDEIKDVKGTLSYMI 300

Query: 301 RNRFPSGNYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEEEFVQAAVDAGEVVWR 360
           +NRFPSGNY SSE  +  D LVHWCHGAPGVALTLVKAA+V+  +EFV+AA++AGEVVW 
Sbjct: 301 QNRFPSGNYLSSEGSKS-DRLVHWCHGAPGVALTLVKAAQVYNTKEFVEAAMEAGEVVWS 360

Query: 361 RGLLKRVGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGD 420
           RGLLKRVGICHG+SGN+YVFLSLY+LT   +YLY+AKAFA FLLD++ +LI+EG+MHGGD
Sbjct: 361 RGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHGGD 410

Query: 421 SRYSLFEGVGGMAHLFLDMIEPSMAKFPGYDL 438
             +SLFEG+GGMA++ LDM +P+ A FPGY+L
Sbjct: 421 RPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410

BLAST of Carg18446 vs. TAIR 10
Match: AT5G65280.1 (GCR2-like 1 )

HSP 1 Score: 309.3 bits (791), Expect = 4.9e-84
Identity = 186/446 (41.70%), Postives = 253/446 (56.73%), Query Frame = 0

Query: 21  VESILSMP-YPIIS---ERFKRAALDLKEAIVLETW--GVTGQR-----VRDFTLYAGAL 80
           ++ +LS P  P IS   E F RAA  LK  +V  TW  GV         V D T+Y G L
Sbjct: 30  IDHLLSEPSAPTISLPTESFLRAATLLKNQVVEATWKGGVEALASGSGPVLDPTVYTGLL 89

Query: 81  GTAFLLLKAHEVTSNHIDLSLCAQIVKACDQASSMSTIRARRLSLEGVDMRRCASKDAGS 140
           GTAF  LK++EVT NH DL  CA+I+  C   +  +T                       
Sbjct: 90  GTAFTCLKSYEVTRNHQDLLTCAEIIDTCANVARATTRH--------------------- 149

Query: 141 QRGGLRGPTSIREENKCWDVTFICGRAGVCAIGAVAAKRAGDEQLLCYYLGQFNEIKLPR 200
                              VTF+CGR GVC +GA+ A   GD+    ++LG F E+   R
Sbjct: 150 -------------------VTFLCGRGGVCTLGAIVANYRGDQSKRDFFLGLFLELAEER 209

Query: 201 NLPD-----------ELLYGKVGFLWACLYLNKHIGEGTVASVHTRAIVEEIIQRGR-AL 260
            LP            +LLYG+ GFLWA L+LN+++G+GTV       IV  I+  GR   
Sbjct: 210 ELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQGTVPDHLLSPIVAAILAGGRVGA 269

Query: 261 AKGGASPLMFEWYGERYWGGAHGLAGILHILMDMELKPDEIQDVKGTLR----NRFP-SG 320
           A   A PL++ ++G R+WG A+GLAGIL++L+   L  ++++DV+GTLR    NRFP SG
Sbjct: 270 ADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLSEEDVKDVQGTLRYMMSNRFPNSG 329

Query: 321 NYPSSEEDRGRDILVHWCHGAPGVALTLVKAAKVFGEE-EFVQAAVDAGEVVWRRGLLKR 380
           NYP S E   RD LV W HGA G+A+TL KA++VF +E +F +AA++AGEVVW+ GL+K+
Sbjct: 330 NYPCS-EGNPRDKLVQWAHGATGMAITLAKASQVFPKERDFREAAIEAGEVVWKSGLVKK 389

Query: 381 VGICHGVSGNSYVFLSLYQLTGKVEYLYKAKAFACFLLDRAHRLIAEGEMHGGDSRYSLF 438
           VG+  GV+GN+Y FLSLY+LTG V Y  +AKAFA +L   A  L+        +  YSLF
Sbjct: 390 VGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLCRDAIELVNMTSQE-TEHDYSLF 433