Carg18328 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18328
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cyclin-dependent protein kinase inhibitor SMR4
Location	Carg_Chr04: 1037220 .. 1037435 (-)
RNA-Seq Expression	Carg18328
Synteny	Carg18328

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAGGAAAAGAAGGATATATGCCGCACGCCGCGACGGAAGAGCTGCCGGATTCCGGTGGCGGTGTTGTGCCCGGCGGCGCCGAGGAAGAAGGCGGTTAGCTTGAAGCGCAAGGCTCCGCCGAAGGACGGGTACTTTCGGCCGCCGGATTTGGAGGTGTTGTTCGGCTTTGTTGCTCCAAGAGGAGATGTATATGTCCATAATAATCTTTGA

mRNA sequence

ATGGCTGAGGAAAAGAAGGATATATGCCGCACGCCGCGACGGAAGAGCTGCCGGATTCCGGTGGCGGTGTTGTGCCCGGCGGCGCCGAGGAAGAAGGCGGTTAGCTTGAAGCGCAAGGCTCCGCCGAAGGACGGGTACTTTCGGCCGCCGGATTTGGAGGTGTTGTTCGGCTTTGTTGCTCCAAGAGGAGATGTATATGTCCATAATAATCTTTGA

Coding sequence (CDS)

ATGGCTGAGGAAAAGAAGGATATATGCCGCACGCCGCGACGGAAGAGCTGCCGGATTCCGGTGGCGGTGTTGTGCCCGGCGGCGCCGAGGAAGAAGGCGGTTAGCTTGAAGCGCAAGGCTCCGCCGAAGGACGGGTACTTTCGGCCGCCGGATTTGGAGGTGTTGTTCGGCTTTGTTGCTCCAAGAGGAGATGTATATGTCCATAATAATCTTTGA

Protein sequence

MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVAPRGDVYVHNNL

Homology

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BLAST of Carg18328 vs. NCBI nr
Match: KAG6600042.1 (Cyclin-dependent protein kinase inhibitor SMR4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030712.1 Cyclin-dependent protein kinase inhibitor SMR4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 149.8 bits (377), Expect = 8.7e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60
          MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA
Sbjct: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60

Query: 61 PRGDVYVHNNL 72
          PRGDVYVHNNL
Sbjct: 61 PRGDVYVHNNL 71

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BLAST of Carg18328 vs. NCBI nr
Match: KAE8647993.1 (hypothetical protein Csa_021483 [Cucumis sativus])

HSP 1 Score: 107.1 bits (266), Expect = 6.5e-20
Identity = 49/62 (79.03%), Postives = 55/62 (88.71%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60
          MAEE  ++CRTPRR+SCRIPVA+ CP+APRKK V LKRKAPPK+GYFR PDLEVLFGFV 
Sbjct: 1  MAEETVELCRTPRRRSCRIPVAMACPSAPRKKPVCLKRKAPPKEGYFRSPDLEVLFGFVT 60

Query: 61 PR 63
          PR
Sbjct: 61 PR 62

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BLAST of Carg18328 vs. NCBI nr
Match: XP_021655298.1 (cyclin-dependent protein kinase inhibitor SMR4-like [Hevea brasiliensis])

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-11
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 3  EEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVAPR 62
          E   ++C TPRR+  RIP A+ CP APRKK V  K+++PPKDGYF+PPDLE LF  +APR
Sbjct: 2  EVDHEVCNTPRRRESRIPAALACPPAPRKKPVFSKKRSPPKDGYFQPPDLEALF-MIAPR 60

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BLAST of Carg18328 vs. NCBI nr
Match: KAE7996760.1 (hypothetical protein FH972_001453 [Carpinus fangiana])

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-10
Identity = 32/60 (53.33%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 6  KDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVAPRGDV 65
          ++ C TPR   CR+P A  CP AP+KK V  K++APPK+GYF+PPDLE LF  V+  G +
Sbjct: 11 EESCGTPRHSGCRLPAAFACPPAPKKKPVYFKQQAPPKNGYFQPPDLEALFAMVSDEGSL 70

[Close]

BLAST of Carg18328 vs. NCBI nr
Match: XP_010256881.1 (PREDICTED: uncharacterized protein LOC104597159 [Nelumbo nucifera] >DAD26462.1 TPA_asm: hypothetical protein HUJ06_027930 [Nelumbo nucifera])

HSP 1 Score: 73.9 bits (180), Expect = 6.1e-10
Identity = 36/66 (54.55%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60
          M EE++  C+TP+   C+IP A +CP  PRKK V  K++ PPK+GYF+PPDLEVLF  +A
Sbjct: 11 MTEEEQG-CQTPKHGECQIPTASVCPPPPRKKPVYGKKRDPPKNGYFQPPDLEVLFA-LA 70

Query: 61 PRGDVY 67
          PR + Y
Sbjct: 71 PRREAY 74

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BLAST of Carg18328 vs. ExPASy Swiss-Prot
Match: Q1JPP8 (Cyclin-dependent protein kinase inhibitor SMR4 OS=Arabidopsis thaliana OX=3702 GN=SMR4 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.7e-10
Identity = 33/62 (53.23%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60
          MAEE    C TPR    RIPVA +CP  PRKK + ++++ PP++GYF+PPDLE LF +  
Sbjct: 10 MAEEG---CTTPRSTMYRIPVASVCPPPPRKKLMVVRKRDPPRNGYFQPPDLETLF-YAQ 67

Query: 61 PR 63
          PR
Sbjct: 70 PR 67

[Close]

BLAST of Carg18328 vs. ExPASy Swiss-Prot
Match: Q9LNX4 (Cyclin-dependent protein kinase inhibitor SMR5 OS=Arabidopsis thaliana OX=3702 GN=SMR5 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 5.0e-07
Identity = 27/55 (49.09%), Postives = 33/55 (60.00%), Query Frame = 0


Query: 9  CRTPRRKSCRIPVAVLCPAAPRKKAVSL---KRKAPPKDGYFRPPDLEVLFGFVA 61
          C TP R  CRIP    CP   R+K   L   K++ PPK GYF+PPDL++ F  VA
Sbjct: 22 CTTPTRDDCRIPAYPPCPPPVRRKRSLLGFGKKREPPKKGYFQPPDLDLFFSVVA 76

[Close]

BLAST of Carg18328 vs. ExPASy Swiss-Prot
Match: Q1G3Y4 (Cyclin-dependent protein kinase inhibitor SMR15 OS=Arabidopsis thaliana OX=3702 GN=SMR15 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 8.0e-05
Identity = 24/54 (44.44%), Postives = 35/54 (64.81%), Query Frame = 0


Query: 4   EKKDIC-RTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLF 57
           E +D+C  TP   S RIP  + CP AP+K+ ++LK    P++ +F PPDLE +F
Sbjct: 49  EDEDLCPTTPTADSVRIPTVIPCPPAPKKRKLTLKVSYGPRE-FFSPPDLETVF 101

[Close]

BLAST of Carg18328 vs. ExPASy TrEMBL
Match: A0A0A0KS84 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G182660 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.1e-20
Identity = 49/62 (79.03%), Postives = 55/62 (88.71%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60
          MAEE  ++CRTPRR+SCRIPVA+ CP+APRKK V LKRKAPPK+GYFR PDLEVLFGFV 
Sbjct: 1  MAEETVELCRTPRRRSCRIPVAMACPSAPRKKPVCLKRKAPPKEGYFRSPDLEVLFGFVT 60

Query: 61 PR 63
          PR
Sbjct: 61 PR 62

[Close]

BLAST of Carg18328 vs. ExPASy TrEMBL
Match: A0A5N6QF01 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_001453 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.7e-11
Identity = 32/60 (53.33%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 6  KDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVAPRGDV 65
          ++ C TPR   CR+P A  CP AP+KK V  K++APPK+GYF+PPDLE LF  V+  G +
Sbjct: 11 EESCGTPRHSGCRLPAAFACPPAPKKKPVYFKQQAPPKNGYFQPPDLEALFAMVSDEGSL 70

[Close]

BLAST of Carg18328 vs. ExPASy TrEMBL
Match: A0A1U8A503 (uncharacterized protein LOC104597159 OS=Nelumbo nucifera OX=4432 GN=LOC104597159 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.9e-10
Identity = 36/66 (54.55%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60
          M EE++  C+TP+   C+IP A +CP  PRKK V  K++ PPK+GYF+PPDLEVLF  +A
Sbjct: 11 MTEEEQG-CQTPKHGECQIPTASVCPPPPRKKPVYGKKRDPPKNGYFQPPDLEVLFA-LA 70

Query: 61 PRGDVY 67
          PR + Y
Sbjct: 71 PRREAY 74

[Close]

BLAST of Carg18328 vs. ExPASy TrEMBL
Match: G7KBS4 (Uncharacterized protein OS=Medicago truncatula OX=3880 GN=MTR_5g080040 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.0e-10
Identity = 33/58 (56.90%), Postives = 43/58 (74.14%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAV--SLKRKAPPKDGYFRPPDLEVLF 57
          M  + ++ CRTP+   CRIP A +CP+AP+KK V  S K+K PPKDGYF PPDLE++F
Sbjct: 3  MKLDMEEGCRTPKHSGCRIPPATICPSAPKKKPVVYSTKKKVPPKDGYFNPPDLELIF 60

[Close]

BLAST of Carg18328 vs. ExPASy TrEMBL
Match: B9T003 (Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_0040060 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.0e-10
Identity = 35/55 (63.64%), Postives = 41/55 (74.55%), Query Frame = 0


Query: 9  CRTPRRKSCRIPVA-VLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVAPR 63
          CRTP R+ CRIP A V CP APRKK V +K++ PPKDGYF+PPDLE  F  + PR
Sbjct: 8  CRTPIRRECRIPAAPVACPPAPRKKPVHIKQRDPPKDGYFQPPDLEAFF-LITPR 61

[Close]

BLAST of Carg18328 vs. TAIR 10
Match: AT5G02220.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 65.1 bits (157), Expect = 2.6e-11
Identity = 33/62 (53.23%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 1  MAEEKKDICRTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLFGFVA 60
          MAEE    C TPR    RIPVA +CP  PRKK + ++++ PP++GYF+PPDLE LF +  
Sbjct: 10 MAEEG---CTTPRSTMYRIPVASVCPPPPRKKLMVVRKRDPPRNGYFQPPDLETLF-YAQ 67

Query: 61 PR 63
          PR
Sbjct: 70 PR 67

[Close]

BLAST of Carg18328 vs. TAIR 10
Match: AT1G60783.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G10690.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 5.7e-06
Identity = 24/54 (44.44%), Postives = 35/54 (64.81%), Query Frame = 0


Query: 4   EKKDIC-RTPRRKSCRIPVAVLCPAAPRKKAVSLKRKAPPKDGYFRPPDLEVLF 57
           E +D+C  TP   S RIP  + CP AP+K+ ++LK    P++ +F PPDLE +F
Sbjct: 49  EDEDLCPTTPTADSVRIPTVIPCPPAPKKRKLTLKVSYGPRE-FFSPPDLETVF 101

[Close]

BLAST of Carg18328 vs. TAIR 10
Match: AT1G10690.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G60783.1); Has 59 Blast hits to 59 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 59; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 40.8 bits (94), Expect = 5.3e-04
Identity = 26/55 (47.27%), Postives = 31/55 (56.36%), Query Frame = 0


Query: 4   EKKDIC-RTPRRKSCRIPVAVLCPAAPRKKAVSLK-RKAPPKDGYFRPPDLEVLF 57
           E++D C  TP   S RIP    CPAAP+K+  SLK         YF PPDLE +F
Sbjct: 50  EEQDQCPTTPTAVSVRIPRVPPCPAAPKKRKPSLKCSYVTVTRDYFSPPDLETVF 104

The following BLAST results are available for this feature:

BLAST of Carg18328 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6600042.1	8.7e-33	100.00	Cyclin-dependent protein kinase inhibitor SMR4, partial [Cucurbita argyrosperma ...	[more]
KAE8647993.1	6.5e-20	79.03	hypothetical protein Csa_021483 [Cucumis sativus]	[more]
XP_021655298.1	1.9e-11	60.00	cyclin-dependent protein kinase inhibitor SMR4-like [Hevea brasiliensis]	[more]
KAE7996760.1	1.6e-10	53.33	hypothetical protein FH972_001453 [Carpinus fangiana]	[more]
XP_010256881.1	6.1e-10	54.55	PREDICTED: uncharacterized protein LOC104597159 [Nelumbo nucifera] >DAD26462.1 T...	[more]

BLAST of Carg18328 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1JPP8	3.7e-10	53.23	Cyclin-dependent protein kinase inhibitor SMR4 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9LNX4	5.0e-07	49.09	Cyclin-dependent protein kinase inhibitor SMR5 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q1G3Y4	8.0e-05	44.44	Cyclin-dependent protein kinase inhibitor SMR15 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg18328 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS84	3.1e-20	79.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G182660 PE=4 SV=1	[more]
A0A5N6QF01	7.7e-11	53.33	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_001453 PE=4 SV=1	[more]
A0A1U8A503	2.9e-10	54.55	uncharacterized protein LOC104597159 OS=Nelumbo nucifera OX=4432 GN=LOC104597159...	[more]
G7KBS4	5.0e-10	56.90	Uncharacterized protein OS=Medicago truncatula OX=3880 GN=MTR_5g080040 PE=4 SV=1	[more]
B9T003	5.0e-10	63.64	Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_0040060 PE=4 SV=1	[more]

BLAST of Carg18328 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02220.1	2.6e-11	53.23	unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -...	[more]
AT1G60783.1	5.7e-06	44.44	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G10690.1	5.3e-04	47.27	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR040389	Cyclin-dependent protein kinase inhibitor SMR	PANTHER	PTHR33142	CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13	coord: 3..62
None	No IPR available	PANTHER	PTHR33142:SF56	CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR5	coord: 3..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18328-RA	Carg18328-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045839	negative regulation of mitotic nuclear division
biological_process	GO:0032875	regulation of DNA endoreduplication
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity