Carg18268 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18268
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein kinase domain-containing protein
LocationCarg_Chr04: 17996952 .. 17999719 (-)
RNA-Seq ExpressionCarg18268
SyntenyCarg18268
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTCTCCCTTTGTACTTGTTCTAACCCAAGGGGATCCCCTCTTGCGTTAGATCATCACCATTGTCAGTATAATGAGGTAAACATTAGAACCCTTTTGAGAAAAATGATTTGGGAACGTGGGTTTGCGTGTATTCTTCCTCCTGCTCGTTTTCGCCGCAAGAATCAGGGGATTCAAGATGATGAACACCCAAATGTAGAAGGAAAAAACGAGAATCTAGAGCATAATAACGCATGGTTGCTAGCGGAATCTGGTGGTTGCGGTGGGGAGTTGTTTAATTCTGATCCTCAGTCGGTTCATTCATCTTTTCGATTCAGTTTTTGCTCTCAGATTGAGCTTGAAACTATGAACATCGCTTCTTCTACACCTCCGGCGACTGTTTTGATGGTGAATTTAGATAATGGAATGATGGCTGAAGCTCTCAACAAAGAATTAAAATGGCGGAGGATTGAATCGCTTGAAAAGAGCATTTCCCCTGTGGCTCATACCTTGATTAGATTCAGCTTCCGGGAAATTCTTTCTGCAACACGTAATTTCTCAACAGGTACTCTCAAAATCAATGACCCTTTTCGCTTCTAAACCAAAATTTTGTTATGTAATATTGAAGTTTCTTCAATGTTGTTGAATGTGGTAGGCAGAGTTCTTGGAAGAGGGGCATTGAGCTGCGTGTTCAGAGGGAGGGTTGGGTTTTTGAGAACTTCTGTGGCCATCAAACGTTTAGACAAGGAAGATAAAGAATCAACGAAGGCATTCTGCAGAGAGTTGATGATTGCTAGCTCTCTTCAGAATCCATTTATAGTTCCTCTTGTTGGGTTCTGCATAGATCCAGAAGAGGGTTTGTTTTTGGTTTATAAATATGTTTCTGGTGGAAGTTTAGAAAGACATTTGCATGGTATGCCTTCTTGTTCTTGTGTTCTTGATTGCTTGAAATTTAGTGAGTTTTTTGAGATTCTAACAGGATAGCCATTGCAGATAAGAAGAAGAGGGGAGTGAGGGGTGGCTTGTCACTTCCTTGGTCGGTGAGATTCAAGATTGGTATTGGAATTGCTGAGGCTGTGGCATATCTCCACAATGGGACTGAAAGATGTGTGGTTCATAGAGACATTAAGCCTTCAAATATCCTTCTTTCATCCAAGAATATGCCAAAGGTGAAAGAATTTTGTTGATTTTGTTCGGATATTCGTTTGGTTGTTTGATGTGCAACGAAGGAAAGAGATAGGGTAGGGAAATAGAGTGTGAGATCCCACGTCGGTTGGAGAGGAGAATGAAGCATTCCTTTTAAGGGTGTGGAAACCTCTCCCCAGTAGAAGCGTTTAAAATCGTGAGGCTGATGACGATACGTAACGGGTCAAAGGAGACAATGTCTGCTAGTGGGGGGGCTTGGACTTTTACAAGTGGCATCGGAGCTAGAAATCATGCGGCGTGCCTGCCTCCAAGGGGATGGATTGTGAGATCCCACATCAGTTGGAGAGGAGAACGAAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAACATACGTGTTTTAAAACCTCGAGTGAAAGCCGAAAAAGGACAATATCTACTAGCGGTGGGCCTGTGTTCGAAAGAAACGAATCAAGTCAACACTCAAAGATAATCTAAATGATCTCTTCAAATAAGTCTAATTACCAAATGTTAACCTTTGTTCTTATTCATCAATGGCTTGCAGTTATGTGATTTTGGGTTAGCAACATGGACTTCTGCTCCATTCGTGCCTTTCCTTTGCAAAACTGTCAAAGGAACATTTGGGTATGAACTCATTCTTGAGATATTCTAACGTAGGGGAAGGACGACGAGAATCTCTCGCCTTCCACGTACGTGTTCCTTTTCGATTTACAATTTTCGATCTTATTTGTAGTTACCTAGCTCCAGAGTATTTCCAGCACGGGAAAGTATCAGATAAAACAGATGTCTACGCTCTAGGAGTCGTGTTATTGGAACTCATAACGGGCAGGAAACCGATCGAAGCAAGAACACATGGAGAAGAGAACTTGGTTCTATGGGTAAGTCATTATATCACTCTAATGACTTCATCTAGTTTTGTTGGACTAGTGATTCTGTTCTGCACAAAGTCATATGTAATAGAACATGACTGTGCTCGTCACTTACCTTTGTAAACGAGCTAAAACAGGCCTCTCTCTATAGGTTTGCCTTTCTGTCTCCGCTTCGAATATTCTAGTATCATATAATTAGCCAAATCGTCTGAAGCACAAACAGAACCACCTTTGTACCGCTAACTATTATAGGAGTTCCCGATCTCACATCCAAGTTGATATAATGTGATTCATATATCGAACTGTACTCCACGAAACCGTTCGTCGGTGCAGTCTATAGTAGATGTTGTAATTGTCCTCATAACTTCAAAAAACTTCATATTTTCGTTCAGGCAAAGCCATTATTGCAGAAAGGGAAAGCAGCGATTACCGAGCTGCTCGATCCACTGGTAGCATGCACGACGAAGAATTCGAATCAAGTAATTCAAATGATCGAAGCTGCAGCTGCCTGCATTACCAGTGAAGAATCGCGCAGGCCCGGGATCTTAGAGATCATTGCAATATTGAAAGGTGAAGAACAGCCTATGCCCTCCAGATCAAAGAGGTCAGGTTTCTTGGGGCAAGGATGTGTCACTGATAGCTATTCTCAGTTTCAAAATACGAACAGCGAGATGATGACGGGTCATTTGGCGTTGGCCATGGCGGGACTAACCGAGTTCGAACAAGATGATTACCTCTTCTGTCGTTAA

mRNA sequence

ATGGGTTTCTCCCTTTGTACTTGTTCTAACCCAAGGGGATCCCCTCTTGCGTTAGATCATCACCATTGTCAGTATAATGAGGTAAACATTAGAACCCTTTTGAGAAAAATGATTTGGGAACGTGGGTTTGCGTGTATTCTTCCTCCTGCTCGTTTTCGCCGCAAGAATCAGGGGATTCAAGATGATGAACACCCAAATGTAGAAGGAAAAAACGAGAATCTAGAGCATAATAACGCATGGTTGCTAGCGGAATCTGGTGGTTGCGGTGGGGAGTTGTTTAATTCTGATCCTCAGTCGGTTCATTCATCTTTTCGATTCAGTTTTTGCTCTCAGATTGAGCTTGAAACTATGAACATCGCTTCTTCTACACCTCCGGCGACTGTTTTGATGGTGAATTTAGATAATGGAATGATGGCTGAAGCTCTCAACAAAGAATTAAAATGGCGGAGGATTGAATCGCTTGAAAAGAGCATTTCCCCTGTGGCTCATACCTTGATTAGATTCAGCTTCCGGGAAATTCTTTCTGCAACACGTAATTTCTCAACAGGCAGAGTTCTTGGAAGAGGGGCATTGAGCTGCGTGTTCAGAGGGAGGGTTGGGTTTTTGAGAACTTCTGTGGCCATCAAACGTTTAGACAAGGAAGATAAAGAATCAACGAAGGCATTCTGCAGAGAGTTGATGATTGCTAGCTCTCTTCAGAATCCATTTATAGTTCCTCTTGTTGGGTTCTGCATAGATCCAGAAGAGGGTTTGTTTTTGGTTTATAAATATGTTTCTGGTGGAAGTTTAGAAAGACATTTGCATGATAAGAAGAAGAGGGGAGTGAGGGGTGGCTTGTCACTTCCTTGGTCGGTGAGATTCAAGATTGGTATTGGAATTGCTGAGGCTGTGGCATATCTCCACAATGGGACTGAAAGATGTGTGGTTCATAGAGACATTAAGCCTTCAAATATCCTTCTTTCATCCAAGAATATGCCAAAGTTATGTGATTTTGGGTTAGCAACATGGACTTCTGCTCCATTCGTGCCTTTCCTTTGCAAAACTGTCAAAGGAACATTTGGTTACCTAGCTCCAGAGTATTTCCAGCACGGGAAAGTATCAGATAAAACAGATGTCTACGCTCTAGGAGTCGTGTTATTGGAACTCATAACGGGCAGGAAACCGATCGAAGCAAGAACACATGGAGAAGAGAACTTGGTTCTATGGGCAAAGCCATTATTGCAGAAAGGGAAAGCAGCGATTACCGAGCTGCTCGATCCACTGGTAGCATGCACGACGAAGAATTCGAATCAAGTAATTCAAATGATCGAAGCTGCAGCTGCCTGCATTACCAGTGAAGAATCGCGCAGGCCCGGGATCTTAGAGATCATTGCAATATTGAAAGGTGAAGAACAGCCTATGCCCTCCAGATCAAAGAGGTCAGGTTTCTTGGGGCAAGGATGTGTCACTGATAGCTATTCTCAGTTTCAAAATACGAACAGCGAGATGATGACGGGTCATTTGGCGTTGGCCATGGCGGGACTAACCGAGTTCGAACAAGATGATTACCTCTTCTGTCGTTAA

Coding sequence (CDS)

ATGGGTTTCTCCCTTTGTACTTGTTCTAACCCAAGGGGATCCCCTCTTGCGTTAGATCATCACCATTGTCAGTATAATGAGGTAAACATTAGAACCCTTTTGAGAAAAATGATTTGGGAACGTGGGTTTGCGTGTATTCTTCCTCCTGCTCGTTTTCGCCGCAAGAATCAGGGGATTCAAGATGATGAACACCCAAATGTAGAAGGAAAAAACGAGAATCTAGAGCATAATAACGCATGGTTGCTAGCGGAATCTGGTGGTTGCGGTGGGGAGTTGTTTAATTCTGATCCTCAGTCGGTTCATTCATCTTTTCGATTCAGTTTTTGCTCTCAGATTGAGCTTGAAACTATGAACATCGCTTCTTCTACACCTCCGGCGACTGTTTTGATGGTGAATTTAGATAATGGAATGATGGCTGAAGCTCTCAACAAAGAATTAAAATGGCGGAGGATTGAATCGCTTGAAAAGAGCATTTCCCCTGTGGCTCATACCTTGATTAGATTCAGCTTCCGGGAAATTCTTTCTGCAACACGTAATTTCTCAACAGGCAGAGTTCTTGGAAGAGGGGCATTGAGCTGCGTGTTCAGAGGGAGGGTTGGGTTTTTGAGAACTTCTGTGGCCATCAAACGTTTAGACAAGGAAGATAAAGAATCAACGAAGGCATTCTGCAGAGAGTTGATGATTGCTAGCTCTCTTCAGAATCCATTTATAGTTCCTCTTGTTGGGTTCTGCATAGATCCAGAAGAGGGTTTGTTTTTGGTTTATAAATATGTTTCTGGTGGAAGTTTAGAAAGACATTTGCATGATAAGAAGAAGAGGGGAGTGAGGGGTGGCTTGTCACTTCCTTGGTCGGTGAGATTCAAGATTGGTATTGGAATTGCTGAGGCTGTGGCATATCTCCACAATGGGACTGAAAGATGTGTGGTTCATAGAGACATTAAGCCTTCAAATATCCTTCTTTCATCCAAGAATATGCCAAAGTTATGTGATTTTGGGTTAGCAACATGGACTTCTGCTCCATTCGTGCCTTTCCTTTGCAAAACTGTCAAAGGAACATTTGGTTACCTAGCTCCAGAGTATTTCCAGCACGGGAAAGTATCAGATAAAACAGATGTCTACGCTCTAGGAGTCGTGTTATTGGAACTCATAACGGGCAGGAAACCGATCGAAGCAAGAACACATGGAGAAGAGAACTTGGTTCTATGGGCAAAGCCATTATTGCAGAAAGGGAAAGCAGCGATTACCGAGCTGCTCGATCCACTGGTAGCATGCACGACGAAGAATTCGAATCAAGTAATTCAAATGATCGAAGCTGCAGCTGCCTGCATTACCAGTGAAGAATCGCGCAGGCCCGGGATCTTAGAGATCATTGCAATATTGAAAGGTGAAGAACAGCCTATGCCCTCCAGATCAAAGAGGTCAGGTTTCTTGGGGCAAGGATGTGTCACTGATAGCTATTCTCAGTTTCAAAATACGAACAGCGAGATGATGACGGGTCATTTGGCGTTGGCCATGGCGGGACTAACCGAGTTCGAACAAGATGATTACCTCTTCTGTCGTTAA

Protein sequence

MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQDDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIASSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDPLVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQGCVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR
Homology
BLAST of Carg18268 vs. NCBI nr
Match: KAG6602032.1 (putative serine/threonine-protein kinase PBL7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032726.1 putative serine/threonine-protein kinase PBL7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1058.9 bits (2737), Expect = 1.4e-305
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ
Sbjct: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA
Sbjct: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF
Sbjct: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL
Sbjct: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL
Sbjct: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY
Sbjct: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP
Sbjct: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
           LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG
Sbjct: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR
Sbjct: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 520

BLAST of Carg18268 vs. NCBI nr
Match: XP_022961095.1 (probable serine/threonine-protein kinase PBL7 [Cucurbita moschata])

HSP 1 Score: 1057.0 bits (2732), Expect = 5.3e-305
Identity = 519/520 (99.81%), Postives = 519/520 (99.81%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ
Sbjct: 59  MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 118

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA
Sbjct: 119 DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 178

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF
Sbjct: 179 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 238

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL
Sbjct: 239 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 298

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL
Sbjct: 299 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 358

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY
Sbjct: 359 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 418

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP
Sbjct: 419 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 478

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
           LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLG G
Sbjct: 479 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGHG 538

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR
Sbjct: 539 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 578

BLAST of Carg18268 vs. NCBI nr
Match: XP_023518494.1 (probable serine/threonine-protein kinase PBL7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.7e-298
Identity = 507/520 (97.50%), Postives = 512/520 (98.46%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSLCTCSNPR SPL LDHHHCQYNEVNIRTLLRKMIWERGFACIL PARFRRKNQGIQ
Sbjct: 59  MGFSLCTCSNPRESPLMLDHHHCQYNEVNIRTLLRKMIWERGFACILSPARFRRKNQGIQ 118

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           DDEHPNVEG NENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNI 
Sbjct: 119 DDEHPNVEGNNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIP 178

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF
Sbjct: 179 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 238

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL
Sbjct: 239 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 298

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRG+RGGLSLPWSVRFKIGIGIAEAVAYL
Sbjct: 299 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGMRGGLSLPWSVRFKIGIGIAEAVAYL 358

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILLSSK MPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY
Sbjct: 359 HNGTERCVVHRDIKPSNILLSSKKMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 418

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLEL+TGRKPIEART GEENLVLWAKPLLQKGKAAITELLDP
Sbjct: 419 FQHGKVSDKTDVYALGVVLLELVTGRKPIEARTRGEENLVLWAKPLLQKGKAAITELLDP 478

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
           LVACTTKNSNQ+IQM+EAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLG G
Sbjct: 479 LVACTTKNSNQIIQMMEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGHG 538

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDY+FCR
Sbjct: 539 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYVFCR 578

BLAST of Carg18268 vs. NCBI nr
Match: XP_022990961.1 (probable serine/threonine-protein kinase PBL7 [Cucurbita maxima])

HSP 1 Score: 1031.9 bits (2667), Expect = 1.8e-297
Identity = 509/520 (97.88%), Postives = 511/520 (98.27%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSLCTCSNPR SPLALDHHH QYNEV IRTLLRKMIWERGFACILPPA FRRKNQGIQ
Sbjct: 59  MGFSLCTCSNPRESPLALDHHHRQYNEVKIRTLLRKMIWERGFACILPPACFRRKNQGIQ 118

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           DDEHPNVEGKNENLEHN AWLLAESG CGGELFNSDPQSVHSSFRFSFCSQIELETMNIA
Sbjct: 119 DDEHPNVEGKNENLEHNKAWLLAESGACGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 178

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF
Sbjct: 179 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 238

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL
Sbjct: 239 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 298

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRG+RG LSLPWSVRFKIGIGIAEAVAYL
Sbjct: 299 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGMRGCLSLPWSVRFKIGIGIAEAVAYL 358

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILLSSK MPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY
Sbjct: 359 HNGTERCVVHRDIKPSNILLSSKKMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 418

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP
Sbjct: 419 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 478

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
           LVACTTKNSNQ+IQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLG G
Sbjct: 479 LVACTTKNSNQIIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGHG 538

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR
Sbjct: 539 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 578

BLAST of Carg18268 vs. NCBI nr
Match: XP_008460521.1 (PREDICTED: serine/threonine-protein kinase CDL1-like [Cucumis melo] >KAA0062329.1 serine/threonine-protein kinase CDL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 934.9 bits (2415), Expect = 3.1e-268
Identity = 460/520 (88.46%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSL TCS  R +PL LDHHHC YNE+ I+TLLRKMIWE GFAC+LPPARFRRKNQGIQ
Sbjct: 1   MGFSLGTCSIQRETPLELDHHHCHYNELKIKTLLRKMIWELGFACVLPPARFRRKNQGIQ 60

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           D+EHPNVEG+N+NLEHN AWLLAESG  GGEL N+DPQSVHSSFRFSFCSQ+ELETMNI 
Sbjct: 61  DEEHPNVEGQNKNLEHNKAWLLAESGAGGGELLNADPQSVHSSFRFSFCSQVELETMNIG 120

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SST  ATVLMVNLDNGMMAEAL KELKWRRIESLEKSISPVAHTLIRF +REILSATRNF
Sbjct: 121 SSTTAATVLMVNLDNGMMAEALTKELKWRRIESLEKSISPVAHTLIRFRYREILSATRNF 180

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           S GRVLGRGALSCVFRGRVGFLRT+VAIKRLDKEDKES KAFCRELMIASSL NP IVPL
Sbjct: 181 SKGRVLGRGALSCVFRGRVGFLRTAVAIKRLDKEDKESAKAFCRELMIASSLHNPNIVPL 240

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLH+ KKRG++G LSLPWSVRFKI +GIAEAVAYL
Sbjct: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHE-KKRGMKGRLSLPWSVRFKIALGIAEAVAYL 300

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILL+SK MPKLCDFGLATWT+AP VPFLCKTVKGTFGYLAPEY
Sbjct: 301 HNGTERCVVHRDIKPSNILLTSKKMPKLCDFGLATWTAAPSVPFLCKTVKGTFGYLAPEY 360

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENL+LWAKPLLQKGK AITELLDP
Sbjct: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLILWAKPLLQKGKGAITELLDP 420

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
            V CTTKNSNQ++QMIEAAAACIT EESRRPGILEIIAILKGEE+P+PSRSKR GFLG G
Sbjct: 421 QVTCTTKNSNQIVQMIEAAAACITGEESRRPGILEIIAILKGEEKPLPSRSKRYGFLGHG 480

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTD YSQFQNTNSE MT HLALAM+GL EFE DDYLFCR
Sbjct: 481 CVTDCYSQFQNTNSE-MTSHLALAMSGLPEFEDDDYLFCR 518

BLAST of Carg18268 vs. ExPASy Swiss-Prot
Match: Q9CAL8 (Proline-rich receptor-like protein kinase PERK13 OS=Arabidopsis thaliana OX=3702 GN=PERK13 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.4e-52
Identity = 120/299 (40.13%), Postives = 176/299 (58.86%), Query Frame = 0

Query: 168 FSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELM 227
           F++ E+   T  FS   +LG G   CV++G++   +  VA+K+L     +  + F  E+ 
Sbjct: 341 FTYEELTDITEGFSKHNILGEGGFGCVYKGKLNDGKL-VAVKQLKVGSGQGDREFKAEVE 400

Query: 228 IASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRF 287
           I S + +  +V LVG+CI   E L L+Y+YV   +LE HLH K      G   L W+ R 
Sbjct: 401 IISRVHHRHLVSLVGYCIADSERL-LIYEYVPNQTLEHHLHGK------GRPVLEWARRV 460

Query: 288 KIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCK 347
           +I IG A+ +AYLH      ++HRDIK +NILL  +   ++ DFGLA    +     +  
Sbjct: 461 RIAIGSAKGLAYLHEDCHPKIIHRDIKSANILLDDEFEAQVADFGLAKLNDST-QTHVST 520

Query: 348 TVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPIEA-RTHGEENLVLWAKPL 407
            V GTFGYLAPEY Q GK++D++DV++ GVVLLELITGRKP++  +  GEE+LV WA+PL
Sbjct: 521 RVMGTFGYLAPEYAQSGKLTDRSDVFSFGVVLLELITGRKPVDQYQPLGEESLVEWARPL 580

Query: 408 LQKG--KAAITELLDPLVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGE 464
           L K       +EL+D  +       N+V +MIE AAAC+     +RP +++++  L  E
Sbjct: 581 LHKAIETGDFSELVDRRLE-KHYVENEVFRMIETAAACVRHSGPKRPRMVQVVRALDSE 629

BLAST of Carg18268 vs. ExPASy Swiss-Prot
Match: Q0WRY5 (Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN=PBL7 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 4.4e-52
Identity = 122/317 (38.49%), Postives = 183/317 (57.73%), Query Frame = 0

Query: 147 KWRRIESLEKSISPVAHTLI--RFSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRT 206
           K R   SL +S S  +  ++   F+F E+ +ATRNF    ++G G    V++G +     
Sbjct: 38  KSRAKSSLSESKSKGSDHIVAQTFTFSELATATRNFRKECLIGEGGFGRVYKGYLASTSQ 97

Query: 207 SVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLE 266
           + AIK+LD    +  + F  E+++ S L +P +V L+G+C D ++ L LVY+Y+  GSLE
Sbjct: 98  TAAIKQLDHNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRL-LVYEYMPLGSLE 157

Query: 267 RHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKN 326
            HLHD       G   L W+ R KI  G A+ + YLH+ T   V++RD+K SNILL    
Sbjct: 158 DHLHDISP----GKQPLDWNTRMKIAAGAAKGLEYLHDKTMPPVIYRDLKCSNILLDDDY 217

Query: 327 MPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELIT 386
            PKL DFGLA          +   V GT+GY APEY   G+++ K+DVY+ GVVLLE+IT
Sbjct: 218 FPKLSDFGLAKLGPVGDKSHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVLLEIIT 277

Query: 387 GRKPIE-ARTHGEENLVLWAKPLLQKGKAAITELLDPLVACTTKNSNQVIQMIEAAAACI 446
           GRK I+ +R+ GE+NLV WA+PL  K +   +++ DP++         + Q +  AA C+
Sbjct: 278 GRKAIDSSRSTGEQNLVAWARPLF-KDRRKFSQMADPMLQ-GQYPPRGLYQALAVAAMCV 337

Query: 447 TSEESRRPGILEIIAIL 461
             + + RP I +++  L
Sbjct: 338 QEQPNLRPLIADVVTAL 347

BLAST of Carg18268 vs. ExPASy Swiss-Prot
Match: Q8RXC8 (Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thaliana OX=3702 GN=RBK2 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 9.8e-52
Identity = 119/302 (39.40%), Postives = 171/302 (56.62%), Query Frame = 0

Query: 164 TLIRFSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSVAIKRLDK-EDKESTKAF 223
           +L  FS  +I  AT NFS   ++GRG  + V++G +   +  +A+KRL K    E T  F
Sbjct: 127 SLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKL-IAVKRLTKGTPDEQTAEF 186

Query: 224 CRELMIASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLP 283
             EL I + + +P     +G CI  E G+ LV++    GSL   LH   K        L 
Sbjct: 187 LSELGIIAHVDHPNTAKFIGCCI--EGGMHLVFRLSPLGSLGSLLHGPSK------YKLT 246

Query: 284 WSVRFKIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFV 343
           WS R+ + +G A+ + YLH G +R ++HRDIK  NILL+    P++CDFGLA W      
Sbjct: 247 WSRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKWLPKQLT 306

Query: 344 PFLCKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLW 403
                  +GTFGY APEYF HG V +KTDV+A GV+LLELITG   ++     +++LVLW
Sbjct: 307 HHNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALD---ESQQSLVLW 366

Query: 404 AKPLLQKGKAAITELLDPLVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKG 463
           AKPLL+  + AI EL+DP +     N  ++I++   A+ CI      RP + +++ +L G
Sbjct: 367 AKPLLE--RKAIKELVDPSLG-DEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELLLG 413

Query: 464 EE 465
            E
Sbjct: 427 HE 413

BLAST of Carg18268 vs. ExPASy Swiss-Prot
Match: Q9ZUE0 (Proline-rich receptor-like protein kinase PERK12 OS=Arabidopsis thaliana OX=3702 GN=PERK12 PE=2 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 2.2e-51
Identity = 117/296 (39.53%), Postives = 175/296 (59.12%), Query Frame = 0

Query: 168 FSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELM 227
           FS+ E+   T+ F+   +LG G   CV++G +   +  VA+K+L     +  + F  E+ 
Sbjct: 359 FSYEELAEITQGFARKNILGEGGFGCVYKGTLQDGKV-VAVKQLKAGSGQGDREFKAEVE 418

Query: 228 IASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRF 287
           I S + +  +V LVG+CI  +  L L+Y+YVS  +LE HLH K      G   L WS R 
Sbjct: 419 IISRVHHRHLVSLVGYCISDQHRL-LIYEYVSNQTLEHHLHGK------GLPVLEWSKRV 478

Query: 288 KIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCK 347
           +I IG A+ +AYLH      ++HRDIK +NILL  +   ++ DFGLA          +  
Sbjct: 479 RIAIGSAKGLAYLHEDCHPKIIHRDIKSANILLDDEYEAQVADFGLARLNDTT-QTHVST 538

Query: 348 TVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPI-EARTHGEENLVLWAKPL 407
            V GTFGYLAPEY   GK++D++DV++ GVVLLEL+TGRKP+ + +  GEE+LV WA+PL
Sbjct: 539 RVMGTFGYLAPEYASSGKLTDRSDVFSFGVVLLELVTGRKPVDQTQPLGEESLVEWARPL 598

Query: 408 LQKG--KAAITELLDPLVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAIL 461
           L K      ++EL+D  +       ++V +MIE AAAC+     +RP +++++  L
Sbjct: 599 LLKAIETGDLSELIDTRLE-KRYVEHEVFRMIETAAACVRHSGPKRPRMVQVVRAL 644

BLAST of Carg18268 vs. ExPASy Swiss-Prot
Match: Q9SGY7 (Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thaliana OX=3702 GN=PERK11 PE=2 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 3.7e-51
Identity = 119/298 (39.93%), Postives = 175/298 (58.72%), Query Frame = 0

Query: 166 IRFSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRE 225
           I F++ E+   T  F    V+G G   CV++G + F    VAIK+L     E  + F  E
Sbjct: 356 IHFTYEELSQITEGFCKSFVVGEGGFGCVYKG-ILFEGKPVAIKQLKSVSAEGYREFKAE 415

Query: 226 LMIASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSV 285
           + I S + +  +V LVG+CI  E+  FL+Y++V   +L+ HLH K          L WS 
Sbjct: 416 VEIISRVHHRHLVSLVGYCIS-EQHRFLIYEFVPNNTLDYHLHGKNLP------VLEWSR 475

Query: 286 RFKIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFL 345
           R +I IG A+ +AYLH      ++HRDIK SNILL  +   ++ DFGLA          +
Sbjct: 476 RVRIAIGAAKGLAYLHEDCHPKIIHRDIKSSNILLDDEFEAQVADFGLARLNDTA-QSHI 535

Query: 346 CKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPIE-ARTHGEENLVLWAK 405
              V GTFGYLAPEY   GK++D++DV++ GVVLLELITGRKP++ ++  GEE+LV WA+
Sbjct: 536 STRVMGTFGYLAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDTSQPLGEESLVEWAR 595

Query: 406 PLLQKG--KAAITELLDPLVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAIL 461
           P L +   K  I+E++DP +      S +V +MIE AA+C+     +RP +++++  L
Sbjct: 596 PRLIEAIEKGDISEVVDPRLENDYVES-EVYKMIETAASCVRHSALKRPRMVQVVRAL 643

BLAST of Carg18268 vs. ExPASy TrEMBL
Match: A0A6J1HB15 (probable serine/threonine-protein kinase PBL7 OS=Cucurbita moschata OX=3662 GN=LOC111461706 PE=4 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 2.6e-305
Identity = 519/520 (99.81%), Postives = 519/520 (99.81%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ
Sbjct: 59  MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 118

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA
Sbjct: 119 DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 178

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF
Sbjct: 179 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 238

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL
Sbjct: 239 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 298

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL
Sbjct: 299 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 358

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY
Sbjct: 359 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 418

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP
Sbjct: 419 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 478

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
           LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLG G
Sbjct: 479 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGHG 538

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR
Sbjct: 539 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 578

BLAST of Carg18268 vs. ExPASy TrEMBL
Match: A0A6J1JPE4 (probable serine/threonine-protein kinase PBL7 OS=Cucurbita maxima OX=3661 GN=LOC111487695 PE=4 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 8.9e-298
Identity = 509/520 (97.88%), Postives = 511/520 (98.27%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSLCTCSNPR SPLALDHHH QYNEV IRTLLRKMIWERGFACILPPA FRRKNQGIQ
Sbjct: 59  MGFSLCTCSNPRESPLALDHHHRQYNEVKIRTLLRKMIWERGFACILPPACFRRKNQGIQ 118

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           DDEHPNVEGKNENLEHN AWLLAESG CGGELFNSDPQSVHSSFRFSFCSQIELETMNIA
Sbjct: 119 DDEHPNVEGKNENLEHNKAWLLAESGACGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 178

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF
Sbjct: 179 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 238

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL
Sbjct: 239 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 298

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRG+RG LSLPWSVRFKIGIGIAEAVAYL
Sbjct: 299 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGMRGCLSLPWSVRFKIGIGIAEAVAYL 358

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILLSSK MPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY
Sbjct: 359 HNGTERCVVHRDIKPSNILLSSKKMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 418

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP
Sbjct: 419 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 478

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
           LVACTTKNSNQ+IQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLG G
Sbjct: 479 LVACTTKNSNQIIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGHG 538

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR
Sbjct: 539 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 578

BLAST of Carg18268 vs. ExPASy TrEMBL
Match: A0A5A7V9E6 (Serine/threonine-protein kinase CDL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G00820 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.5e-268
Identity = 460/520 (88.46%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSL TCS  R +PL LDHHHC YNE+ I+TLLRKMIWE GFAC+LPPARFRRKNQGIQ
Sbjct: 1   MGFSLGTCSIQRETPLELDHHHCHYNELKIKTLLRKMIWELGFACVLPPARFRRKNQGIQ 60

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           D+EHPNVEG+N+NLEHN AWLLAESG  GGEL N+DPQSVHSSFRFSFCSQ+ELETMNI 
Sbjct: 61  DEEHPNVEGQNKNLEHNKAWLLAESGAGGGELLNADPQSVHSSFRFSFCSQVELETMNIG 120

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SST  ATVLMVNLDNGMMAEAL KELKWRRIESLEKSISPVAHTLIRF +REILSATRNF
Sbjct: 121 SSTTAATVLMVNLDNGMMAEALTKELKWRRIESLEKSISPVAHTLIRFRYREILSATRNF 180

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           S GRVLGRGALSCVFRGRVGFLRT+VAIKRLDKEDKES KAFCRELMIASSL NP IVPL
Sbjct: 181 SKGRVLGRGALSCVFRGRVGFLRTAVAIKRLDKEDKESAKAFCRELMIASSLHNPNIVPL 240

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLH+ KKRG++G LSLPWSVRFKI +GIAEAVAYL
Sbjct: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHE-KKRGMKGRLSLPWSVRFKIALGIAEAVAYL 300

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILL+SK MPKLCDFGLATWT+AP VPFLCKTVKGTFGYLAPEY
Sbjct: 301 HNGTERCVVHRDIKPSNILLTSKKMPKLCDFGLATWTAAPSVPFLCKTVKGTFGYLAPEY 360

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENL+LWAKPLLQKGK AITELLDP
Sbjct: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLILWAKPLLQKGKGAITELLDP 420

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
            V CTTKNSNQ++QMIEAAAACIT EESRRPGILEIIAILKGEE+P+PSRSKR GFLG G
Sbjct: 421 QVTCTTKNSNQIVQMIEAAAACITGEESRRPGILEIIAILKGEEKPLPSRSKRYGFLGHG 480

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTD YSQFQNTNSE MT HLALAM+GL EFE DDYLFCR
Sbjct: 481 CVTDCYSQFQNTNSE-MTSHLALAMSGLPEFEDDDYLFCR 518

BLAST of Carg18268 vs. ExPASy TrEMBL
Match: A0A1S3CD54 (serine/threonine-protein kinase CDL1-like OS=Cucumis melo OX=3656 GN=LOC103499321 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.5e-268
Identity = 460/520 (88.46%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSL TCS  R +PL LDHHHC YNE+ I+TLLRKMIWE GFAC+LPPARFRRKNQGIQ
Sbjct: 1   MGFSLGTCSIQRETPLELDHHHCHYNELKIKTLLRKMIWELGFACVLPPARFRRKNQGIQ 60

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           D+EHPNVEG+N+NLEHN AWLLAESG  GGEL N+DPQSVHSSFRFSFCSQ+ELETMNI 
Sbjct: 61  DEEHPNVEGQNKNLEHNKAWLLAESGAGGGELLNADPQSVHSSFRFSFCSQVELETMNIG 120

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SST  ATVLMVNLDNGMMAEAL KELKWRRIESLEKSISPVAHTLIRF +REILSATRNF
Sbjct: 121 SSTTAATVLMVNLDNGMMAEALTKELKWRRIESLEKSISPVAHTLIRFRYREILSATRNF 180

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           S GRVLGRGALSCVFRGRVGFLRT+VAIKRLDKEDKES KAFCRELMIASSL NP IVPL
Sbjct: 181 SKGRVLGRGALSCVFRGRVGFLRTAVAIKRLDKEDKESAKAFCRELMIASSLHNPNIVPL 240

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLH+ KKRG++G LSLPWSVRFKI +GIAEAVAYL
Sbjct: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHE-KKRGMKGRLSLPWSVRFKIALGIAEAVAYL 300

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILL+SK MPKLCDFGLATWT+AP VPFLCKTVKGTFGYLAPEY
Sbjct: 301 HNGTERCVVHRDIKPSNILLTSKKMPKLCDFGLATWTAAPSVPFLCKTVKGTFGYLAPEY 360

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENL+LWAKPLLQKGK AITELLDP
Sbjct: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLILWAKPLLQKGKGAITELLDP 420

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQG 480
            V CTTKNSNQ++QMIEAAAACIT EESRRPGILEIIAILKGEE+P+PSRSKR GFLG G
Sbjct: 421 QVTCTTKNSNQIVQMIEAAAACITGEESRRPGILEIIAILKGEEKPLPSRSKRYGFLGHG 480

Query: 481 CVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           CVTD YSQFQNTNSE MT HLALAM+GL EFE DDYLFCR
Sbjct: 481 CVTDCYSQFQNTNSE-MTSHLALAMSGLPEFEDDDYLFCR 518

BLAST of Carg18268 vs. ExPASy TrEMBL
Match: A0A0A0KNA9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G420310 PE=4 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.2e-265
Identity = 458/521 (87.91%), Postives = 481/521 (92.32%), Query Frame = 0

Query: 1   MGFSLCTCSNPRGSPLALDHHHCQYNEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQ 60
           MGFSL TCS  R +PL LDHHHC+YNE+ I+TLLRKMIWE GFACILPPARFRRKNQGIQ
Sbjct: 1   MGFSLGTCSIQRETPLELDHHHCRYNELKIKTLLRKMIWELGFACILPPARFRRKNQGIQ 60

Query: 61  DDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSDPQSVHSSFRFSFCSQIELETMNIA 120
           D+EHPNVEG+N+NLEHN AWLLAESG  GGEL N+DPQSVHSSFRFSFCSQ+ELETMNI 
Sbjct: 61  DEEHPNVEGQNKNLEHNKAWLLAESGAGGGELLNTDPQSVHSSFRFSFCSQVELETMNIG 120

Query: 121 SSTPPATVLMVNLDNGMMAEALNKELKWRRIESLEKSISPVAHTLIRFSFREILSATRNF 180
           SST  ATVLMVNLDNGMMAEAL KELKWRRIESLEKSISPVAHTLIRF +REILSATRNF
Sbjct: 121 SSTTAATVLMVNLDNGMMAEALTKELKWRRIESLEKSISPVAHTLIRFRYREILSATRNF 180

Query: 181 STGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPL 240
           S GRVLGRGALSCVFRGRVGFLRT+VAIKRLDKEDKES KAFCRELMIASSL NP IVPL
Sbjct: 181 SKGRVLGRGALSCVFRGRVGFLRTAVAIKRLDKEDKESAKAFCRELMIASSLHNPNIVPL 240

Query: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRFKIGIGIAEAVAYL 300
           VGFCIDPEEGLFLVYKYVSGGSLERHLH+ KKRG++G  SLPWSVRFKI +GIAEAVAYL
Sbjct: 241 VGFCIDPEEGLFLVYKYVSGGSLERHLHE-KKRGMKGRFSLPWSVRFKIALGIAEAVAYL 300

Query: 301 HNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEY 360
           HNGTERCVVHRDIKPSNILL+SK MPKLCDFGLATWT+AP VPFLCKTVKGTFGYLAPEY
Sbjct: 301 HNGTERCVVHRDIKPSNILLTSKKMPKLCDFGLATWTAAPSVPFLCKTVKGTFGYLAPEY 360

Query: 361 FQHGKVSDKTDVYALGVVLLELITGRKPIEARTHGEENLVLWAKPLLQKGKAAITELLDP 420
           FQHGKVSDKTDVYA+GVVLLELITGRKP EAR HGEENLVLWAKPLLQKGK AITELLDP
Sbjct: 361 FQHGKVSDKTDVYAVGVVLLELITGRKPFEARAHGEENLVLWAKPLLQKGKGAITELLDP 420

Query: 421 LVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQ-PMPSRSKRSGFLGQ 480
            V CTTKNSNQ++QMIEAAAACIT EESRRPGILEIIAILKGEE+ P+PSRSKR  FLG 
Sbjct: 421 QVTCTTKNSNQIVQMIEAAAACITGEESRRPGILEIIAILKGEEKAPLPSRSKRYSFLGH 480

Query: 481 GCVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           GCVTD YSQFQNTNSE MTGHLALAM+GL EFE DDYLFCR
Sbjct: 481 GCVTDCYSQFQNTNSE-MTGHLALAMSGLPEFEDDDYLFCR 519

BLAST of Carg18268 vs. TAIR 10
Match: AT5G37790.1 (Protein kinase superfamily protein )

HSP 1 Score: 615.1 bits (1585), Expect = 5.0e-176
Identity = 327/515 (63.50%), Postives = 383/515 (74.37%), Query Frame = 0

Query: 26  NEVNIRTLLRKMIWERGFACILPPARFRRKNQGIQD--DEHPNVEGKNENLEHNNAWLLA 85
           N   I+TLL++MI++ GFAC LPP     +N G  +         G     E+N AWLLA
Sbjct: 43  NNTGIKTLLKRMIFDLGFACFLPPLS-STENSGNNNGGGTSGGGGGGGGGGENNKAWLLA 102

Query: 86  ESGGCGGELFNSDPQSVHSSFRFSFCSQIELETM--------------NIASSTPPATVL 145
           E+     E  N DP SVHSSFRFS CSQIELE M              N++ S    TVL
Sbjct: 103 ET---APENMNPDPHSVHSSFRFSLCSQIELEKMKGEEPSLSASSSCRNLSVSGGSTTVL 162

Query: 146 MVNLDNGMMAEALN--KELKWRRIESLEKSISPVAHTLIRFSFREILSATRNFSTGRVLG 205
           MVNL+NG+     N   +L+W R  SLEKSISPVA+TL+RFS+ EI++ATRNFS GRVLG
Sbjct: 163 MVNLENGVKETTGNATDDLRWTRARSLEKSISPVANTLVRFSYGEIVAATRNFSKGRVLG 222

Query: 206 RGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELMIASSLQNPFIVPLVGFCIDP 265
           RGA S VFRG++G  RT++AIKRLDKEDKES K+FCRELMIASSL +  IVPL+GFCIDP
Sbjct: 223 RGACSYVFRGKIGMWRTALAIKRLDKEDKESPKSFCRELMIASSLHSSNIVPLLGFCIDP 282

Query: 266 EEGLFLVYKYVSGGSLERHLHD-KKKRGVRGGLSLPWSVRFKIGIGIAEAVAYLHNGTER 325
           EEGLFLVYKYVSGGSLE +LHD KKK+GV+    LPWS R+K+ +GIA+A+AYLHNGTE+
Sbjct: 283 EEGLFLVYKYVSGGSLEHYLHDKKKKKGVKAAFGLPWSARYKVALGIADAIAYLHNGTEQ 342

Query: 326 CVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEYFQHGKV 385
           CVVHRDIKPSNILLSSK +PKLCDFGLATWT+AP VPFLCKTVKGTFGYLAPEYFQHGK+
Sbjct: 343 CVVHRDIKPSNILLSSKKIPKLCDFGLATWTAAPSVPFLCKTVKGTFGYLAPEYFQHGKI 402

Query: 386 SDKTDVYALGVVLLELITGRKPIEA-RTHGEENLVLWAKPLLQKGKAAITELLDPLVACT 445
           SDKTDVYA GVVLLELITGRKPIEA R  G+ENLV+WAKPLL +G  AI ELLDP + CT
Sbjct: 403 SDKTDVYAFGVVLLELITGRKPIEARRASGQENLVVWAKPLLDRGIEAIVELLDPRLKCT 462

Query: 446 TKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEEQPMPSRSKRSGFLGQGCVTDS 505
            KNS Q+ +MI AAAAC+ +EESRRPG+ EI++ILKGEE   P             + DS
Sbjct: 463 RKNSVQMERMIRAAAACVINEESRRPGMEEIVSILKGEEGVEPETHSSRKTTNISGMIDS 522

Query: 506 YSQFQNTNSEMMTGHLALAMAGLTEFEQDDYLFCR 521
           Y+Q Q T SEM   HL LAM G+TEFE DD+++ R
Sbjct: 523 YTQLQQTKSEMKC-HLDLAMLGVTEFEDDDHVYGR 552

BLAST of Carg18268 vs. TAIR 10
Match: AT1G66460.1 (Protein kinase superfamily protein )

HSP 1 Score: 562.8 bits (1449), Expect = 2.9e-160
Identity = 307/497 (61.77%), Postives = 362/497 (72.84%), Query Frame = 0

Query: 37  MIWERGFACILPPARFRRKNQGIQDDEHPNVEGKNENLEHNNAWLLAESGGCGGELFNSD 96
           MI + GF C +PP                    +  + E N AWLLAE+     +L +S+
Sbjct: 1   MILDLGFPCFVPP--------------------RTSSREDNKAWLLAET---EPKLIDSE 60

Query: 97  PQSVHSSFRFSFCSQIELETM----------NIASSTPPATVLMVNLDNGMMAEALNKEL 156
             S+ SSFRFS CSQ+ELE +          N   S    TVL+VNL+N      L  E+
Sbjct: 61  QHSLQSSFRFSLCSQLELEKIKKEKPSLSYRNFPVSEGSETVLLVNLEN--ETGELTGEM 120

Query: 157 KWRRIESLEKSISPVAHTLIRFSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSV 216
            W R  SLEKSISPVA +LIRFS+RE+L+ATRNFS  RVLGRGA S VF+GR+G  R +V
Sbjct: 121 NWSRGLSLEKSISPVADSLIRFSYRELLTATRNFSKRRVLGRGACSYVFKGRIGIWRKAV 180

Query: 217 AIKRLDKEDKESTKAFCRELMIASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERH 276
           AIKRLDK+DKES K+FCRELMIASSL +P +VPL+GFCIDP++GLFLVYKYVSGGSLER 
Sbjct: 181 AIKRLDKKDKESPKSFCRELMIASSLNSPNVVPLLGFCIDPDQGLFLVYKYVSGGSLERF 240

Query: 277 LHDKKKRGVR-GGLSLPWSVRFKIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNM 336
           LHDKKK+  R   L+LPWS R+K+ +GIA+A+AYLHNGTE+CVVHRDIKPSNILLSS  +
Sbjct: 241 LHDKKKKKSRKTPLNLPWSTRYKVALGIADAIAYLHNGTEQCVVHRDIKPSNILLSSNKI 300

Query: 337 PKLCDFGLATWTSAPFVPFLCKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITG 396
           PKLCDFGLATWT+AP VPFLCKTVKGTFGYLAPEYFQHGK+SDKTDVYA GVVLLELITG
Sbjct: 301 PKLCDFGLATWTAAPSVPFLCKTVKGTFGYLAPEYFQHGKISDKTDVYAFGVVLLELITG 360

Query: 397 RKPIEA-RTHGEENLVLWAKPLLQKGKAAITELLDPLVACTTKNSNQVIQMIEAAAACIT 456
           RKPIEA R  GEENLV+WAKPLL +G  A  ELLDP + CT KNS  + +MI AAAAC+ 
Sbjct: 361 RKPIEARRPSGEENLVVWAKPLLHRGIEATEELLDPRLKCTRKNSASMERMIRAAAACVI 420

Query: 457 SEESRRPGILEIIAILKGEE----QPMPSRSKRSGFLGQGCVTDSYSQFQNTNSEMMTGH 516
           +EESRRPG+ EI++ILKG E    + + SR K+S   G   + D Y Q Q T SEM + H
Sbjct: 421 NEESRRPGMKEILSILKGGEGIELRTLSSR-KKSNLPG---IMDCYPQLQRTKSEMKS-H 467

Query: 517 LALAMAGLTEFEQDDYL 518
           L LAM G+TEFE DD L
Sbjct: 481 LTLAMLGVTEFEADDLL 467

BLAST of Carg18268 vs. TAIR 10
Match: AT1G77280.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 253.4 bits (646), Expect = 3.8e-67
Identity = 150/356 (42.13%), Postives = 209/356 (58.71%), Query Frame = 0

Query: 152 ESLEKSISPVAHTLIRFSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSVAIKRL 211
           E LE      + T   F ++E++S T NFS    +G+G  S VFRG +   R  VA+K L
Sbjct: 417 EELEGLYERFSSTCRFFKYKELVSVTSNFSADNFIGKGGSSRVFRGCLSNGRV-VAVKIL 476

Query: 212 DKEDKESTKAFCRELMIASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKK 271
            K+ ++    F  E+ I ++L +  I+ L+GFC + +  L LVY Y+S GSLE +LH  K
Sbjct: 477 -KQTEDVLNDFVAEIEIITTLHHKNIISLLGFCFE-DHNLLLVYNYLSRGSLEENLHGNK 536

Query: 272 KRGVRGGLSLPWSVRFKIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDF 331
           K      L+  WS R+K+ +G+AEA+ YLHN   + V+HRD+K SNILLS    P+L DF
Sbjct: 537 K----DPLAFCWSERYKVAVGVAEALDYLHNTASQPVIHRDVKSSNILLSDDFEPQLSDF 596

Query: 332 GLATWTSAPFVPFLCKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPIEA 391
           GLA W S      +C  V GTFGYLAPEYF +GKV+DK DVYA GVVLLEL++GRKPI +
Sbjct: 597 GLARWASISTTHIICSDVAGTFGYLAPEYFMYGKVNDKIDVYAFGVVLLELLSGRKPISS 656

Query: 392 R-THGEENLVLWAKPLLQKGKAAITELLDP-LVACTTKNSNQVIQMIEAAAACITSEESR 451
               G+E+LV+WAKP+L  GK   ++LLDP L      N +Q+ +M  AA  CI      
Sbjct: 657 GCPKGQESLVMWAKPILDDGK--YSQLLDPSLRDNNNNNDDQMQRMALAATLCIRRSPQA 716

Query: 452 RPGILEIIAILKGEEQPMPSRSKRSGFLGQGCVTDSYSQFQNTNSEMMTGHLALAM 506
           RP +  ++ +LKG+E  +    ++     +        Q Q +N   +  HL LA+
Sbjct: 717 RPKMSIVLKLLKGDEDTLEWAMQQVNSSSEESEMLKDEQCQRSN---LQSHLNLAL 760

BLAST of Carg18268 vs. TAIR 10
Match: AT4G35030.3 (Protein kinase superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.4e-61
Identity = 148/352 (42.05%), Postives = 207/352 (58.81%), Query Frame = 0

Query: 168 FSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLRTSVAIKRLDKEDKESTKAFCRELM 227
           F++  +  AT +FS   V+G+G  + V+RG +   +  +A+K L    KE+   F  E+ 
Sbjct: 92  FNYNVLRKATSDFSQENVIGKGGCNEVYRGILEDGK-GIAVKILKSSSKEAMTNFVHEIN 151

Query: 228 IASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSVRF 287
           I SSL +  I PL+G C+   E L  VY   + GSLE  LH K+K    G   L W  RF
Sbjct: 152 IISSLSHQNISPLLGVCVQDNE-LISVYNLSNTGSLEETLHGKQK----GKYVLSWEERF 211

Query: 288 KIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATW---TSAPFVPF 347
           KI IG+AEA+ YLHN   + V+HRD+K SN+LLS +  P+L DFGL+ W   TS+ +   
Sbjct: 212 KIAIGLAEALDYLHNRCSKPVIHRDVKTSNVLLSLELQPQLSDFGLSMWGPTTSSRY--S 271

Query: 348 LCKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPIEART-HGEENLVLWA 407
           +   V GTFGYLAPEYF +GKVSDK DVYA GVVLLELI+GR PI  +   G+E+LV+WA
Sbjct: 272 IQGDVVGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLELISGRNPISPQNPRGQESLVMWA 331

Query: 408 KPLLQKGKAAITELLDPLVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGE 467
           KPL+  G   +  LLDP V     + +Q  +M+ AA+ C+T   + RP I +I+ +L+ E
Sbjct: 332 KPLIDTGNLKV--LLDPDVT-DIFDESQFQRMVLAASHCLTRSATHRPNIRQILRLLRDE 391

Query: 468 EQPMPSRSKRSGFLGQGCVTDSYSQFQNTNSEMMTGHLALAMAGLTEFEQDD 516
            +      +  G   + C  D    + N+++E+   HL LAM    E E D+
Sbjct: 392 NEAGKWIMEEEG--NEDCFDDEV--YPNSSTEL---HLNLAM---LEVEDDE 422

BLAST of Carg18268 vs. TAIR 10
Match: AT2G16750.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 232.6 bits (592), Expect = 7.0e-61
Identity = 146/350 (41.71%), Postives = 201/350 (57.43%), Query Frame = 0

Query: 168 FSFREILSATRNFSTGRVLGRGALSCVFRGRVGFLR--TSVAIKRLDKEDKESTKAFCRE 227
           FS+  + +AT +FS   ++G+G  + V++   GFL     VA+K L    KE+ K F  E
Sbjct: 265 FSYDVLKTATSDFSLENLIGKGGCNEVYK---GFLEDGKGVAVKILKPSVKEAVKEFVHE 324

Query: 228 LMIASSLQNPFIVPLVGFCIDPEEGLFLVYKYVSGGSLERHLHDKKKRGVRGGLSLPWSV 287
           + I SSL +  I PL+G C+   + L  VY   S GSLE  L  K          L W  
Sbjct: 325 VSIVSSLSHSNISPLIGVCVHYND-LISVYNLSSKGSLEETLQGKH--------VLRWEE 384

Query: 288 RFKIGIGIAEAVAYLHNGTERCVVHRDIKPSNILLSSKNMPKLCDFGLATWTSAPFVPFL 347
           R KI IG+ EA+ YLHN     V+HRD+K SN+LLS +  P+L DFGL+ W S      +
Sbjct: 385 RLKIAIGLGEALDYLHNQCSNPVIHRDVKSSNVLLSDEFEPQLSDFGLSMWGSKSCRYTI 444

Query: 348 CKTVKGTFGYLAPEYFQHGKVSDKTDVYALGVVLLELITGRKPIEART-HGEENLVLWAK 407
            + V GTFGYLAPEYF +GKVSDK DVYA GVVLLELI+GR  I + +  G+E+LV+WAK
Sbjct: 445 QRDVVGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLELISGRTSISSDSPRGQESLVMWAK 504

Query: 408 PLLQKGKAAITELLDPLVACTTKNSNQVIQMIEAAAACITSEESRRPGILEIIAILKGEE 467
           P+++KG A   ELLDP +A  T + +Q  +M+ AA  C+T   + RP I EI+ +L+GE+
Sbjct: 505 PMIEKGNA--KELLDPNIA-GTFDEDQFHKMVLAATHCLTRAATYRPNIKEILKLLRGED 564

Query: 468 QPMP----SRSKRSGFLGQGCVTDSYSQFQNTNSEMMTGHLALAMAGLTE 511
                         GF            + N+N+E+   HL+LAM  + +
Sbjct: 565 DVSKWVKIEEDDEDGF--------DDEVYPNSNTEL---HLSLAMVDVED 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6602032.11.4e-305100.00putative serine/threonine-protein kinase PBL7, partial [Cucurbita argyrosperma s... [more]
XP_022961095.15.3e-30599.81probable serine/threonine-protein kinase PBL7 [Cucurbita moschata][more]
XP_023518494.11.7e-29897.50probable serine/threonine-protein kinase PBL7 [Cucurbita pepo subsp. pepo][more]
XP_022990961.11.8e-29797.88probable serine/threonine-protein kinase PBL7 [Cucurbita maxima][more]
XP_008460521.13.1e-26888.46PREDICTED: serine/threonine-protein kinase CDL1-like [Cucumis melo] >KAA0062329.... [more]
Match NameE-valueIdentityDescription
Q9CAL83.4e-5240.13Proline-rich receptor-like protein kinase PERK13 OS=Arabidopsis thaliana OX=3702... [more]
Q0WRY54.4e-5238.49Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8RXC89.8e-5239.40Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thal... [more]
Q9ZUE02.2e-5139.53Proline-rich receptor-like protein kinase PERK12 OS=Arabidopsis thaliana OX=3702... [more]
Q9SGY73.7e-5139.93Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thalian... [more]
Match NameE-valueIdentityDescription
A0A6J1HB152.6e-30599.81probable serine/threonine-protein kinase PBL7 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JPE48.9e-29897.88probable serine/threonine-protein kinase PBL7 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7V9E61.5e-26888.46Serine/threonine-protein kinase CDL1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CD541.5e-26888.46serine/threonine-protein kinase CDL1-like OS=Cucumis melo OX=3656 GN=LOC10349932... [more]
A0A0A0KNA91.2e-26587.91Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G420... [more]
Match NameE-valueIdentityDescription
AT5G37790.15.0e-17663.50Protein kinase superfamily protein [more]
AT1G66460.12.9e-16061.77Protein kinase superfamily protein [more]
AT1G77280.13.8e-6742.13Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT4G35030.31.4e-6142.05Protein kinase superfamily protein [more]
AT2G16750.17.0e-6141.71Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 180..459
e-value: 2.0E-38
score: 143.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 181..457
e-value: 5.6E-44
score: 150.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 180..468
score: 36.414143
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 165..257
e-value: 6.2E-20
score: 73.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 258..474
e-value: 1.4E-52
score: 180.1
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 181..394
e-value: 6.7E-25
score: 85.9
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 182..464
e-value: 5.5E-11
score: 39.8
NoneNo IPR availablePANTHERPTHR47989:SF33SERINE/THREONINE-PROTEIN KINASE CDL1coord: 19..520
NoneNo IPR availablePANTHERPTHR47989OS01G0750732 PROTEINcoord: 19..520
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 308..320
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 161..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18268-RACarg18268-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity