Carg18239 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18239
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	transmembrane protein 50A-like
Location	Carg_Chr04: 18176819 .. 18182759 (+)
RNA-Seq Expression	Carg18239
Synteny	Carg18239

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGGAAGAATCCACCAATGGATTTGGCGGAGCTTTGGTCAATTTTCGGCCCTGGCGTTGCCGGAGCTGTGTTCGGCGCCGGGTGGTGGTTCTGGGTTGACGCCGTTGTCTGCAGTTCTGTTGCCGTCTCCTTCCTCCACTATCTTCCAGGTTTTTTATTCATTTACCAGTCCACACCATGTATAATTTGCAGTTAGGGTTTTTTATGTTGTTTTGGTATTGGGGTTTTAGGTATTTTTGCTTCGTTTGCGGCTCTGATGTTCAATTGCGTTCGGAAGGAAGACATCGATTATTCACCATATGAGGAAGGCGAATGGAGGTGAGTATGTTATGATTTATCTGCTTACCTCTCTCTTTCTATCTCTTGTTGTTTTCAGCCGATTTTATTGTTTTAAATGGCACCAATCATACCGCCATCCTCTTTGCCTGATCATTCGATTCGATTGAAAGCAATGTCACAGTGTTTTTTCTTCAACAAGTCATTGCTCAATGTGATTTAGTGTCTTGCTAGCATTATTCTCCCTGGATGAGTTAGGATTTCATGAACTCTTACATCCGAGACTTAATAAGAAAATGTTAATTTATTGTATCCAATTAGAATACCCGTAACGATAGGTCGAAATCTCCATGCTGGATAAAAATACTAAATATAACCATCTAAAAGATAGAGTATTATTTTAAATCGTTACTTGCAGAGAGAACTAAAGCAAAAAAAAGCAACATGCTCTTCGTGTGTCGTCGTGACCTAATAAATGATCTGATATTGTATCAGATTAAAACAAGCTCGAAGTTGATTCGCACCGTTAATTCACGGATAACACATTGCGTTGGAGGCTAATGATAGAGGACATAGACTGCTTAGAAAATAATAATCTTTCTATCTCTTATGATATTCTAGATTTGTCGTTTCTAAACGTTACTTTAGAAGATAGGTCTTTTATATTGTCAGTATCTAGTCCATAATCCTCTTATACTGTCTTCATTGCTGGTAGTATATTGTGTCATGTCTGACCTATGCTAAGCATGGTCACAGTGGCATATTCAAAAACATTCATGCTCAATGTGTATTAGTGTTAATGTAAACTGGCAGATATTCTCCCTGGCGAAGATCATAGTTAGGATTTCATGAACACTTACATTGACGAGGACATATTCTTGGAATTGGCACATGAACATTTATAGTTAATACCGTAAACACGATAGTGTCTGCGATTCAGCCGTCGAGATATCAACTTCACAAAGTGATTGTGACGACGTGACTATATCCTAGTTTATTCGCGTTTGTAGGTCAACCTGGCTGTAAGTATACGATGTTGTTCAGTATATGTGTCTTATTGGTGATTAGACCGAGAGTTGATACATCCCAGAGATATCTATAGACGATCCTTTCAGTCTCAAACAATCTATGTTCAATATAGAGTTAGAAGGAAAGTCAAATTTATGTTTCTCTAGAACTCAATTGGGTTATGGGTTAAGGCCTCAGCTCATTCGATTGGGTCCGAGGGTGGGCTTGCTTTCATAGCATCTTGGCCTTCTAAGAGACTTTAGGTCAGTCATTCTTTGTATCTTTAAATTATTTATTTAGGTTGCAGTATAGACATTTCACATCCTGAATTAATCTCAATTCTGTATTGATCTACTCAGGAAGAAAAGCTATGTCTCGTTGAAGCGAGGTTAAGACTTATTAAATATTGATCCCAGCGAATCCTTAGTGAAAATATAGCTCGTGTTCTATTTTCTCTTATACAAACTTGTGTTTTGGATCCCATGTGATTAGAGTTTGGATGTAGTTCCGTCGGGAAGACTTATGTGAAGACCTTTTACGGATCTCATGGAGGATAGGCATTTAGGGTGCACATTATATGTTACTTTACACCTGGTCCAGGATCATGTGGGCTATCTTTCAGGATGTGTTATAGGCAGTCTTTGTATTCTTCGGAAGTGGTGCAGTATTAATCCTTGAAGAAAGAATCGCTCTCCCTGTAGTTTCAAGTAATTAATCCACTATTATTACAATGAGGGAGAGATGCATCTTTAGTAATTCTCTCGTCATCATTAGACGCACTATGAGGAGCTCCTCTGTAGTCACGTCTTACAAATAATATCTCGATCCAGGAATTTATTTCAAGTTCTAGCGACTCTTTTCCTTACTCTGGAAGATGAGCAAGATACTAAATAGATAGAAGAGATTGCGATCATCTCTTTTGGTACTGAATGTGTAGCAGTTTACTTTGATTGTTGTGGGGTGGCAAATTTGTTAGAGACGTTTATTCTACGGGTAGGCTTGGTCTCAGGAGTCGGCCGTGTGTATGAGCGAGCTATATCTGGGTATCATTTATATATATGTTTTTTCTATATTATATGTGAATCCTCCTTTATTAGCACGAGAACCTAAAATGTGCAGAGATCTGTAGAGAGTGGAGAATCTAAAGTGCTAAGGTTCTATGATTCATGATATTTCATTTTTTTATAAGATACCGTTTCATTAATGTTTGAGATTTACAAATATTATACTTATAAGTAATATCTTGAAGAAAATAACTAATAATCCTGTTATAACGAACCATGTATTATCCATGCCCTTACTAATAAGGACATGTTCGCCAATTTAACAATCAGGTAAGGTTGTAGGAAGACAACTTACACTATTTCTTGGTAAACAACATTGTTGAAGAGCCTAGTAGGAGTTTCCAATAAGATTCATTGAATTCACTCTTTCCAAATATTCCAAAAGAAAATCATGATGAGGTTGGGTCTATACCAGAGTTTTTGCATTCTTGAAAGGGGGTATGTTAAGACCATGTCCAGTTAATCTTTGACCTCCTTAGGAACAGTGAGGTGTCATCCGAAAATGGTGAGAATATTTATCCATAAGTTATAGGCATAAGTACACTACATAAATAGGTGGTTTTTGGATTCATTCTCTCTTGCATAAAGGGCATTAATTGGAAAATAGAGTCATGCAGGGCATTCATTTCTGTAGGTTTTCATTTGTGCTAATGGCTTTGTGCATAACTTCTCATGGGAAAAACTCCACCTTTCTTTATGATTTCCTTTCCATATTGTCTTGGGCTATTGTGAGGTTTATGCCTTTTATTTTTTCCCCCATATCTATCATTAAGGATTTTGTGGAGATAACCGATCCCTAGGAAGGCATTTCAATGAGCCTACTTCTTGGGATAACACTATTGGGCAAGGTCAAGGCTTAGCTTGGCCCATTATGTAGCTTTGCTATCCTTGAGGTTCGTATTGAGCTGTGACTCCCAAAATTTGTTGTCATATTTCCATATTTCTTTGATTGTGGTCGTCTTGCAATGTGAGTGCTATATAAAAGAGGATACTGCAATGCTAGCATGGTGTTGGAATCCAAGATGTGTTTTCTCCATCACCCACCCTATGGCAAATGCGGTTAGTGATGAGGTTTTGGTGCTCTTTTATAAACTTTCTGGTCCATTGGCAGAAGATGGGAATAATCGGTTTGTACATGATGTTGGAGCGTGTTTAGCTTTTATAAGATTCCTCCACAAGGCAGTGCCTTCAAGTGAGGTATGGTCACTTGTTAGATTTCACACCTCTCTTGCGTCTCTGTTTGTAAAGATTTTTGTCATTACCCCATAGGTCTTATTATGCATGACTAGAGCTGGTTGGGCTCTTCTTGGGTTTTTCGTGTTTTCTTTTTTGTTGGTATTCCTCGTGTATTCTTCCTTTTTTTTTTCCACAGTGAAAACTCACATTTTACCCCATAAAAATCCCCTTTTCAACCTTCTCCCCAATTGGGTGGGTTGTGAAAAGGAGGAACTAAGGGGCAAGAGTGAGGTTCGTTAGGTTTGAGAACATGCTTAGTGAGAGAGAGCTGAAAGAAGAGCATGTGTATGCAGAAAATATCATAAACGAAGACATGGAACGTAACTTCTTGATAGAATAGTCCAAGGATAGTAGACATAGGTGGAGATCCAACAACCATCGAAAAATGATGTATTAGAGGTTGTGGATCTCTGCGAAGGTATAAGAATAATATTCAGAATGCTATTATATAGAAATAAAATTTTCATGCATCATCCCTCCCAAACTTTAAAAGTCACGACCCCAAGAATTTTCATCAATCATAGGTCCACCAATTTAGGGAGGCAAACCAATAACCCAAGTTCTAGCAAAAGAAAAGACCAGCTCTTAAAAGAAAAGGAGCAATAGAGGAAAATGGTCTAGAGATTCGTCGTCCTTCTTACAAAGACAACAAGCCAAGGGGGACAAAGCCAGAGTGGGAACTTCTGTAATTTGACGGCAGGATTAACTATTAAGATTGAGACTTCTATAGGTGAGACACAAAAGAAATGTTCACTTTCTTAGGGACTTGAAATTCCAAATATAAACCACGAAAGGGGCCTTGAACTTAGCAGGAGGTTTGGTAAGGTAAGATGCATGGCAAACCATTTCCTCACACCTTCAGCAGTTGTGATACTTGTTTGAGAATAAAATTACTAGTGTGCAAGCTGAAATCTAGCTTGTAGGAAATTCATTAACACCCACTTAAAAATGGATTTCATGAAGTGGTAATTGTAGAATTCAAATCTGCATAGAAGGCCTAATGCATAAAGAAATCTTTTCATATGCCCTAAGCTTCCATACTAGTCTACAATATTGGTTCATACTAGGTAGGACATGATGAATTGATATGTTAGTTATTTGTTGAGATATCACGGAAATAAGCCTCTGTAAATTCAAGTTTCAGAGACCTATAAGCAAAGGAAAATTAGAAGACTTGTGTGATAGCATCGAGAATTTAATGTCTGTTTTGATGTGGTGCTAGTGTTTTTCTGTAATTAACAGTACTGACGTTTCCCTTAACTGCAGATTAAAGCTTTGGCTCTTCTTCGCTTATGTGGTATCCTTCGTTTCATTAGCAGCATCTGTGGGGCTCTTGATTCAAGATTCAGTAGTAAAAACTGGCCTATCAGCATGGACGGGAATTGCAGGAGTATTGCAGTGTGTGTTTGTGTTGGTTAGGTAAATGGCCTCTCTCCTTTTTATTATCTTAATTCTGATTTTTCCCATCTGTCTTTTAGTTGGATTTGTGCCAGCAAACATGTAGCCACTTCTTATTGTCTACTCCACCTAGTTGTGGAATCATTCAGCAAGGCAACTTTGTTAAAGAAGAGTTGTTGGGGTTCTTTCATTATGTTGTTTCTCTTGATTTCTAAGCATGTCTGAAACTACTACATAAGATGTTTAAATCATGCCATAAACAAAGTTGGTTGAACGAGAGTATTTTCTCTTTAGCAGTCTGATGCTGTATCAATATATGCCCTGTTCTTTGGTCTTCGTTGCATTAAATAGCTTGTGGAAAAACCAAAATCATTAAGGGGTTTCAATTTTCATCACTCGCACAAGTGTCATGTAATACAAAAATGCTACGTAGCTAGACCCTGGTCAGCACTGTCTATGTTCTGTTTTCCTGAAATACTGTTTGTTGCAGTGGGTTGATTTACTGGACTTCTCACACGGAATAAGTCGGAAAGAATTATGCACTTAGACCTCAGAGGCTGAAGAGTTACCAACTGCATAGCGCGTTGCATCGTCAAGTCGATTGTGTGTCACTTGACTTTTCCAACTATGGAACTGAACAGTAAGAGCTTTCTACTTCTTGTAAATGTCATGTTAATCTTAAGCTGAAACCATGTCTGTAAATCTTTATGAATCCAATATAAGTGAGCAAGTGAGCCAGCATTCAATGTTTCAGCCATTTCAGTTTGTCGCAGTGTTCATATTGTATACTTACAAAGCATAAAAATGATGCAGGGTGGTCAGTTTTGTTTTGGTTTTTAGTTTCTTTCCTTCGAAAAATTGTGGATGCAAAATGAATTAGATATATTTTAATCTGCCGAAATTCATTAATTGAAAGGTTATCTTATTACACCGTTTTATTTTATTTTTTTATTAACA

mRNA sequence

AAAAGGAAGAATCCACCAATGGATTTGGCGGAGCTTTGGTCAATTTTCGGCCCTGGCGTTGCCGGAGCTGTGTTCGGCGCCGGGTGGTGGTTCTGGGTTGACGCCGTTGTCTGCAGTTCTGTTGCCGTCTCCTTCCTCCACTATCTTCCAGGTATTTTTGCTTCGTTTGCGGCTCTGATGTTCAATTGCGTTCGGAAGGAAGACATCGATTATTCACCATATGAGGAAGGCGAATGGAGATTAAAGCTTTGGCTCTTCTTCGCTTATGTGGTATCCTTCGTTTCATTAGCAGCATCTGTGGGGCTCTTGATTCAAGATTCAGTAGTAAAAACTGGCCTATCAGCATGGACGGGAATTGCAGGAGTATTGCAGTGTGTGTTTGTGTTGGTTAGTGGGTTGATTTACTGGACTTCTCACACGGAATAAGTCGGAAAGAATTATGCACTTAGACCTCAGAGGCTGAAGAGTTACCAACTGCATAGCGCGTTGCATCGTCAAGTCGATTGTGTGTCACTTGACTTTTCCAACTATGGAACTGAACAGTAAGAGCTTTCTACTTCTTGTAAATGTCATGTTAATCTTAAGCTGAAACCATGTCTGTAAATCTTTATGAATCCAATATAAGTGAGCAAGTGAGCCAGCATTCAATGTTTCAGCCATTTCAGTTTGTCGCAGTGTTCATATTGTATACTTACAAAGCATAAAAATGATGCAGGGTGGTCAGTTTTGTTTTGGTTTTTAGTTTCTTTCCTTCGAAAAATTGTGGATGCAAAATGAATTAGATATATTTTAATCTGCCGAAATTCATTAATTGAAAGGTTATCTTATTACACCGTTTTATTTTATTTTTTTATTAACA

Coding sequence (CDS)

ATGGATTTGGCGGAGCTTTGGTCAATTTTCGGCCCTGGCGTTGCCGGAGCTGTGTTCGGCGCCGGGTGGTGGTTCTGGGTTGACGCCGTTGTCTGCAGTTCTGTTGCCGTCTCCTTCCTCCACTATCTTCCAGGTATTTTTGCTTCGTTTGCGGCTCTGATGTTCAATTGCGTTCGGAAGGAAGACATCGATTATTCACCATATGAGGAAGGCGAATGGAGATTAAAGCTTTGGCTCTTCTTCGCTTATGTGGTATCCTTCGTTTCATTAGCAGCATCTGTGGGGCTCTTGATTCAAGATTCAGTAGTAAAAACTGGCCTATCAGCATGGACGGGAATTGCAGGAGTATTGCAGTGTGTGTTTGTGTTGGTTAGTGGGTTGATTTACTGGACTTCTCACACGGAATAA

Protein sequence

MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRKEDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCVFVLVSGLIYWTSHTE

Homology

[Close]

BLAST of Carg18239 vs. NCBI nr
Match: KAG7032755.1 (Transmembrane protein 50A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 277.3 bits (708), Expect = 6.9e-71
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSHTE
Sbjct: 121 FVLVSGLIYWTSHTE 135

[Close]

BLAST of Carg18239 vs. NCBI nr
Match: XP_022963347.1 (transmembrane protein 50A-like [Cucurbita moschata] >XP_022991142.1 transmembrane protein 50A-like [Cucurbita maxima] >XP_023517110.1 transmembrane protein 50A-like [Cucurbita pepo subsp. pepo] >KAG6602062.1 Transmembrane protein 50A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276.9 bits (707), Expect = 9.0e-71
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSHTE
Sbjct: 121 FVLVSGLIYWTSHTE 135

[Close]

BLAST of Carg18239 vs. NCBI nr
Match: XP_038874577.1 (transmembrane protein 50A [Benincasa hispida])

HSP 1 Score: 270.4 bits (690), Expect = 8.4e-69
Identity = 130/135 (96.30%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSF+HYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFIHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTG S WTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGPSVWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSH+E
Sbjct: 121 FVLVSGLIYWTSHSE 135

[Close]

BLAST of Carg18239 vs. NCBI nr
Match: XP_008460472.1 (PREDICTED: transmembrane protein 50A [Cucumis melo])

HSP 1 Score: 270.0 bits (689), Expect = 1.1e-68
Identity = 130/135 (96.30%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSF+HYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFVHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTG S WTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGPSVWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSH+E
Sbjct: 121 FVLVSGLIYWTSHSE 135

[Close]

BLAST of Carg18239 vs. NCBI nr
Match: XP_022993057.1 (transmembrane protein 50A [Cucurbita maxima] >XP_023549618.1 transmembrane protein 50A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268.9 bits (686), Expect = 2.4e-68
Identity = 129/135 (95.56%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSF+HYLPGIFASFAALMFNCVR+
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFVHYLPGIFASFAALMFNCVRR 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTG S WTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGPSVWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSH+E
Sbjct: 121 FVLVSGLIYWTSHSE 135

[Close]

BLAST of Carg18239 vs. ExPASy Swiss-Prot
Match: Q9CXL1 (Transmembrane protein 50A OS=Mus musculus OX=10090 GN=Tmem50a PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-09
Identity = 44/140 (31.43%), Postives = 64/140 (45.71%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLH--YLPGIFASFAALMFNCV 60
           MD  E  +      AG +F  GWW  +DA V       F H  +  G+ A+ A LM N V
Sbjct: 14  MDWGEKRNTIASIAAGVLFFTGWWIIIDAAVMYPRMDQFNHSYHTCGVIATIAFLMINAV 73

Query: 61  RKEDIDYSPYEE---GEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAG 120
               +    Y E   G+   ++WLF  ++++F SL AS+ +L    V K     + GIA 
Sbjct: 74  SNGQVRGDSYSEGCLGQTGARIWLFIGFMLAFGSLIASMWILFGGYVAKEKDVVYPGIAV 133

Query: 121 VLQCVFVLVSGLIYWTSHTE 136
             Q  F+   GL++    TE
Sbjct: 134 FFQNAFIFFGGLVFKFGRTE 153

[Close]

BLAST of Carg18239 vs. ExPASy Swiss-Prot
Match: O95807 (Transmembrane protein 50A OS=Homo sapiens OX=9606 GN=TMEM50A PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-09
Identity = 41/126 (32.54%), Postives = 60/126 (47.62%), Query Frame = 0


Query: 15  AGAVFGAGWWFWVDAVVCSSVAVSFLH--YLPGIFASFAALMFNCVRKEDIDYSPYEE-- 74
           AG +F  GWW  +DA V       F H  +  G+ A+ A LM N V    +    Y E  
Sbjct: 28  AGVLFFTGWWIIIDAAVIYPTMKDFNHSYHACGVIATIAFLMINAVSNGQVRGDSYSEGC 87

Query: 75  -GEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCVFVLVSGLIY 134
            G+   ++WLF  ++++F SL AS+ +L    V K     + GIA   Q  F+   GL++
Sbjct: 88  LGQTGARIWLFVGFMLAFGSLIASMWILFGGYVAKEKDIVYPGIAVFFQNAFIFFGGLVF 147

Query: 135 WTSHTE 136
               TE
Sbjct: 148 KFGRTE 153

[Close]

BLAST of Carg18239 vs. ExPASy Swiss-Prot
Match: Q54T60 (Transmembrane protein 50 homolog OS=Dictyostelium discoideum OX=44689 GN=tmem50 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.7e-08
Identity = 35/132 (26.52%), Postives = 69/132 (52.27%), Query Frame = 0


Query: 12  PGVAGAVFGAGWWFWVDAVVCSSV----------AVSFLHYLPGIFASFAALMFNCVRKE 71
           P +AG +F AGW+ W+D  V  +            + +++YLPGIFA+   +M N V   
Sbjct: 10  PALAGIIFTAGWFLWIDGHVYENTNNKNADFDGPHIQWIYYLPGIFATLGMVMANIVDLS 69

Query: 72  DIDYSP--YEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVV---KTGLSAWTGIAGV 129
            ++ +   ++ G  ++++WLF ++ +SF  + A++ +++   +        + W GIA  
Sbjct: 70  ALNSNSLLFDGGATKVRVWLFISFAISFGCIGAALWIMVAVFLPPHNTNDAAQWPGIAIT 129

[Close]

BLAST of Carg18239 vs. ExPASy Swiss-Prot
Match: Q9D1X9 (Transmembrane protein 50B OS=Mus musculus OX=10090 GN=Tmem50b PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.5e-07
Identity = 41/140 (29.29%), Postives = 63/140 (45.00%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLH--YLPGIFASFAALMFNCV 60
           +D +E  +     VAG +F  GWW  +DA V         H  +  G+F++ A  M N V
Sbjct: 16  IDWSERRNTVASVVAGILFFTGWWIMIDAAVVYPKPEQLNHAFHTCGVFSTLAFFMINAV 75

Query: 61  RKEDIDYSPYEE---GEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAG 120
               +    YE    G    ++WLF  +++ F SL AS+ +L   + V   +  + G+A 
Sbjct: 76  SNAQVRGDSYESGCLGRTGARVWLFIGFMLMFGSLIASMWILF-GAYVTQNIDVYPGLAV 135

Query: 121 VLQCVFVLVSGLIYWTSHTE 136
             Q   +  S LIY    TE
Sbjct: 136 FFQNALIFFSTLIYKFGRTE 154

[Close]

BLAST of Carg18239 vs. ExPASy Swiss-Prot
Match: Q3SZL9 (Transmembrane protein 50B OS=Bos taurus OX=9913 GN=TMEM50B PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 5.6e-07
Identity = 41/140 (29.29%), Postives = 62/140 (44.29%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLH--YLPGIFASFAALMFNCV 60
           +D +E  +     VAG +F  GWW  +DA V         H  +  G+F++ A  M N V
Sbjct: 16  IDWSERRNAVASVVAGILFFTGWWIMIDAAVVYPKPEQLNHAFHTCGVFSTLAFFMINAV 75

Query: 61  RKEDIDYSPYEE---GEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAG 120
               +    YE    G    ++WLF  +++ F SL AS+ +L   + V      + G+A 
Sbjct: 76  SNAQVRGDSYESGCLGRTGARVWLFIGFMLMFGSLIASMWILF-GAYVTQNTDVYPGLAV 135

Query: 121 VLQCVFVLVSGLIYWTSHTE 136
             Q   +  S LIY    TE
Sbjct: 136 FFQNALIFFSTLIYKFGRTE 154

[Close]

BLAST of Carg18239 vs. ExPASy TrEMBL
Match: A0A6J1JKY2 (transmembrane protein 50A-like OS=Cucurbita maxima OX=3661 GN=LOC111487827 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 4.3e-71
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSHTE
Sbjct: 121 FVLVSGLIYWTSHTE 135

[Close]

BLAST of Carg18239 vs. ExPASy TrEMBL
Match: A0A6J1HF20 (transmembrane protein 50A-like OS=Cucurbita moschata OX=3662 GN=LOC111463579 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 4.3e-71
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSHTE
Sbjct: 121 FVLVSGLIYWTSHTE 135

[Close]

BLAST of Carg18239 vs. ExPASy TrEMBL
Match: A0A1S3CCJ8 (transmembrane protein 50A OS=Cucumis melo OX=3656 GN=LOC103499276 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 5.3e-69
Identity = 130/135 (96.30%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSF+HYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFVHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTG S WTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGPSVWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSH+E
Sbjct: 121 FVLVSGLIYWTSHSE 135

[Close]

BLAST of Carg18239 vs. ExPASy TrEMBL
Match: A0A6J1JRP9 (transmembrane protein 50A OS=Cucurbita maxima OX=3661 GN=LOC111489187 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-68
Identity = 129/135 (95.56%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSF+HYLPGIFASFAALMFNCVR+
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFVHYLPGIFASFAALMFNCVRR 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTG S WTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGPSVWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSH+E
Sbjct: 121 FVLVSGLIYWTSHSE 135

[Close]

BLAST of Carg18239 vs. ExPASy TrEMBL
Match: A0A6J1CJN8 (transmembrane protein 50A OS=Momordica charantia OX=3673 GN=LOC111012019 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.6e-68
Identity = 128/135 (94.81%), Postives = 132/135 (97.78%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSV VSF+HYLPGIFASFAALMFNCVRK
Sbjct: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVPVSFVHYLPGIFASFAALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDS+VKTG S WTG+AGVLQCV
Sbjct: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSIVKTGPSVWTGVAGVLQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVLVSGLIYWTSH+E
Sbjct: 121 FVLVSGLIYWTSHSE 135

[Close]

BLAST of Carg18239 vs. TAIR 10
Match: AT1G36980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0220 (InterPro:IPR007919); Has 424 Blast hits to 424 proteins in 159 species: Archae - 0; Bacteria - 0; Metazoa - 228; Fungi - 120; Plants - 62; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 248.4 bits (633), Expect = 3.2e-66
Identity = 115/135 (85.19%), Postives = 125/135 (92.59%), Query Frame = 0


Query: 1   MDLAELWSIFGPGVAGAVFGAGWWFWVDAVVCSSVAVSFLHYLPGIFASFAALMFNCVRK 60
           MDLAELW+IFGPG +GAVFG GWWFWVDAVVCSS+ V F+HYLPGIFAS  ALMFNCVRK
Sbjct: 1   MDLAELWAIFGPGFSGAVFGTGWWFWVDAVVCSSIQVPFVHYLPGIFASLGALMFNCVRK 60

Query: 61  EDIDYSPYEEGEWRLKLWLFFAYVVSFVSLAASVGLLIQDSVVKTGLSAWTGIAGVLQCV 120
           EDIDYSPY+EGEWRLKLWLF AYVV+FVSLAASVGLLIQDSVVKTG S WTG+AGV QCV
Sbjct: 61  EDIDYSPYDEGEWRLKLWLFIAYVVAFVSLAASVGLLIQDSVVKTGPSTWTGVAGVFQCV 120

Query: 121 FVLVSGLIYWTSHTE 136
           FVL+SGL+YWTSH+E
Sbjct: 121 FVLISGLMYWTSHSE 135

The following BLAST results are available for this feature:

BLAST of Carg18239 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032755.1	6.9e-71	100.00	Transmembrane protein 50A [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022963347.1	9.0e-71	99.26	transmembrane protein 50A-like [Cucurbita moschata] >XP_022991142.1 transmembran...	[more]
XP_038874577.1	8.4e-69	96.30	transmembrane protein 50A [Benincasa hispida]	[more]
XP_008460472.1	1.1e-68	96.30	PREDICTED: transmembrane protein 50A [Cucumis melo]	[more]
XP_022993057.1	2.4e-68	95.56	transmembrane protein 50A [Cucurbita maxima] >XP_023549618.1 transmembrane prote...	[more]

BLAST of Carg18239 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9CXL1	1.6e-09	31.43	Transmembrane protein 50A OS=Mus musculus OX=10090 GN=Tmem50a PE=1 SV=1	[more]
O95807	2.7e-09	32.54	Transmembrane protein 50A OS=Homo sapiens OX=9606 GN=TMEM50A PE=1 SV=1	[more]
Q54T60	1.7e-08	26.52	Transmembrane protein 50 homolog OS=Dictyostelium discoideum OX=44689 GN=tmem50 ...	[more]
Q9D1X9	2.5e-07	29.29	Transmembrane protein 50B OS=Mus musculus OX=10090 GN=Tmem50b PE=1 SV=1	[more]
Q3SZL9	5.6e-07	29.29	Transmembrane protein 50B OS=Bos taurus OX=9913 GN=TMEM50B PE=2 SV=1	[more]

BLAST of Carg18239 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKY2	4.3e-71	99.26	transmembrane protein 50A-like OS=Cucurbita maxima OX=3661 GN=LOC111487827 PE=3 ...	[more]
A0A6J1HF20	4.3e-71	99.26	transmembrane protein 50A-like OS=Cucurbita moschata OX=3662 GN=LOC111463579 PE=...	[more]
A0A1S3CCJ8	5.3e-69	96.30	transmembrane protein 50A OS=Cucumis melo OX=3656 GN=LOC103499276 PE=3 SV=1	[more]
A0A6J1JRP9	1.2e-68	95.56	transmembrane protein 50A OS=Cucurbita maxima OX=3661 GN=LOC111489187 PE=3 SV=1	[more]
A0A6J1CJN8	2.6e-68	94.81	transmembrane protein 50A OS=Momordica charantia OX=3673 GN=LOC111012019 PE=3 SV...	[more]

BLAST of Carg18239 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36980.1	3.2e-66	85.19	unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007919	Uncharacterised protein family UPF0220	PFAM	PF05255	UPF0220	coord: 13..133 e-value: 2.4E-22 score: 79.2
IPR007919	Uncharacterised protein family UPF0220	PANTHER	PTHR13180	SMALL MEMBRANE PROTEIN-RELATED	coord: 3..135
None	No IPR available	PANTHER	PTHR13180:SF3	OS02G0566900 PROTEIN	coord: 3..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18239-RA	Carg18239-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway
cellular_component	GO:0016021	integral component of membrane