Carg18219 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg18219 vs. NCBI nr
Match: KAG7032775.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.1e-302
Identity = 508/508 (100.00%), Postives = 508/508 (100.00%), Query Frame = 0

Query: 1   MCHLTPCPSGFNSNGSTKLTLPFAHLLLLLGAGYILKIRIFMMGEIGGNDYNYFFFDNRK 60
           MCHLTPCPSGFNSNGSTKLTLPFAHLLLLLGAGYILKIRIFMMGEIGGNDYNYFFFDNRK
Sbjct: 1   MCHLTPCPSGFNSNGSTKLTLPFAHLLLLLGAGYILKIRIFMMGEIGGNDYNYFFFDNRK 60

Query: 61  HYRTHIFVPLVVKQIGSVIMELIELGVDTIMVPGNLPIGCLPIYLQVYATSNRGQYEPQN 120
           HYRTHIFVPLVVKQIGSVIMELIELGVDTIMVPGNLPIGCLPIYLQVYATSNRGQYEPQN
Sbjct: 61  HYRTHIFVPLVVKQIGSVIMELIELGVDTIMVPGNLPIGCLPIYLQVYATSNRGQYEPQN 120

Query: 121 GCLKWLNQFSKYHNEQLQQELKRIRALHPHVHLIYADYFNALMDILNAPKNFGFTNLTKF 180
           GCLKWLNQFSKYHNEQLQQELKRIRALHPHVHLIYADYFNALMDILNAPKNFGFTNLTKF
Sbjct: 121 GCLKWLNQFSKYHNEQLQQELKRIRALHPHVHLIYADYFNALMDILNAPKNFGFTNLTKF 180

Query: 181 VYAIETLFNRDRDTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEF 240
           VYAIETLFNRDRDTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEF
Sbjct: 181 VYAIETLFNRDRDTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEF 240

Query: 241 GKGLNFAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDM 300
           GKGLNFAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDM
Sbjct: 241 GKGLNFAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDM 300

Query: 301 FKKSLFIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGN 360
           FKKSLFIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGN
Sbjct: 301 FKKSLFIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGN 360

Query: 361 FPIGCVPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIY 420
           FPIGCVPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIY
Sbjct: 361 FPIGCVPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIY 420

Query: 421 ADYFNAAMRLFNAPKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGL 480
           ADYFNAAMRLFNAPKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGL
Sbjct: 421 ADYFNAAMRLFNAPKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGL 480

Query: 481 HLTEATYELIAISVLEDIFTAPQFSIFQ 509
           HLTEATYELIAISVLEDIFTAPQFSIFQ
Sbjct: 481 HLTEATYELIAISVLEDIFTAPQFSIFQ 508

BLAST of Carg18219 vs. NCBI nr
Match: KAG6594558.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 651.7 bits (1680), Expect = 5.0e-183
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT
Sbjct: 59  ETFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 118

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG
Sbjct: 119 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 178

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL
Sbjct: 179 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 238

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN
Sbjct: 239 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 298

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 492
           APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI
Sbjct: 299 APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 358

Query: 493 SVLEDIFTAPQFSIFQ 509
           SVLEDIFTAPQFSIFQ
Sbjct: 359 SVLEDIFTAPQFSIFQ 374

BLAST of Carg18219 vs. NCBI nr
Match: XP_022926917.1 (GDSL esterase/lipase At1g28570-like [Cucurbita moschata])

HSP 1 Score: 631.3 bits (1627), Expect = 7.1e-177
Identity = 306/316 (96.84%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLG EGISAEEF KGLNFAVGGAT
Sbjct: 70  ETFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGEEGISAEEFRKGLNFAVGGAT 129

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG
Sbjct: 130 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 189

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAI  LIELGVDTIMVPGNFPIGCVPMYLKL
Sbjct: 190 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIRELIELGVDTIMVPGNFPIGCVPMYLKL 249

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQEL++I AL+PHVHLIYADYFNAAMRLFN
Sbjct: 250 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELERIGALHPHVHLIYADYFNAAMRLFN 309

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 492
           APKNFGF+DIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI
Sbjct: 310 APKNFGFVDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 369

Query: 493 SVLEDIFTAPQFSIFQ 509
           SVLEDIFTAPQFSIFQ
Sbjct: 370 SVLEDIFTAPQFSIFQ 385

BLAST of Carg18219 vs. NCBI nr
Match: XP_023518482.1 (GDSL esterase/lipase At1g28570-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 534.6 bits (1376), Expect = 9.0e-148
Identity = 251/316 (79.43%), Postives = 281/316 (88.92%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISA++FGKGLNFAVGGAT
Sbjct: 62  ETFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAQDFGKGLNFAVGGAT 121

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL++SYFEERG+HNVPSHAVSL IQL WF KTYSSVCASS SS C+D+FK SLF+MGEIG
Sbjct: 122 ALDVSYFEERGIHNVPSHAVSLRIQLHWFNKTYSSVCASSPSSRCRDIFKNSLFMMGEIG 181

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN F FD RNI E+ SLVPLV+KQIGS IM LIELGVDTIMVPGN PIGC+P+YL++
Sbjct: 182 GNDYNYFFFDNRNITELTSLVPLVVKQIGSVIMELIELGVDTIMVPGNLPIGCLPIYLQV 241

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           + TSN   YD +NGCLKWLNQFS+YHNEQLQQELK+IRAL+PHVHLIYADYFNA M + N
Sbjct: 242 YATSNRGQYDPQNGCLKWLNQFSKYHNEQLQQELKRIRALHPHVHLIYADYFNAVMHILN 301

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 492
           APKNFGF ++ QVCCVD NR+  FP+PCGLPSTIVCDDPSK+ SWDGLHLTEA Y+LIA 
Sbjct: 302 APKNFGFTNLHQVCCVDENRSYYFPMPCGLPSTIVCDDPSKYVSWDGLHLTEAAYKLIAT 361

Query: 493 SVLEDIFTAPQFSIFQ 509
           S+++  F  PQFSI Q
Sbjct: 362 SLMQGSFMTPQFSILQ 377

BLAST of Carg18219 vs. NCBI nr
Match: KAG6594559.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 534.3 bits (1375), Expect = 1.2e-147
Identity = 251/316 (79.43%), Postives = 282/316 (89.24%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVG+GGEGISAEEFGKGLNFAVGGAT
Sbjct: 60  ETFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGMGGEGISAEEFGKGLNFAVGGAT 119

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL++SYFEERG+HNVPSHAVSL IQL WF KTYSSVC+SS SS C+D+FK SLF+MGEIG
Sbjct: 120 ALDVSYFEERGIHNVPSHAVSLGIQLQWFNKTYSSVCSSSPSSRCRDIFKNSLFMMGEIG 179

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN F FD RNI E+ SLVPLV+KQIGS IM LIELGVDTIMVPGN PIGC+P+YL++
Sbjct: 180 GNDYNYFFFDNRNITELTSLVPLVVKQIGSVIMELIELGVDTIMVPGNLPIGCLPIYLQV 239

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           + TSNG  YD +NGCLKWLNQFS+YHN+QLQQELK IRAL+PHVHLIYADYFNA M + N
Sbjct: 240 YATSNGGQYDPQNGCLKWLNQFSKYHNKQLQQELKLIRALHPHVHLIYADYFNALMDILN 299

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 492
           APKNFGF ++ QVCCVD NR+  FP+PCGLPSTIVCDDPSK+ SWDGLHLTEATY+LIA 
Sbjct: 300 APKNFGFTNLHQVCCVDENRSFYFPMPCGLPSTIVCDDPSKYVSWDGLHLTEATYKLIAT 359

Query: 493 SVLEDIFTAPQFSIFQ 509
           ++++   T PQFSI Q
Sbjct: 360 NLMQGSSTTPQFSILQ 375

BLAST of Carg18219 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 315.1 bits (806), Expect = 1.5e-84
Identity = 159/314 (50.64%), Postives = 209/314 (66.56%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFH PTGR+SDGR+I+DFIA  LG PLV P+        G     F KG+NFAV GAT
Sbjct: 69  ETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPF-------YGCQNANFKKGVNFAVAGAT 128

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL  S+ EERG+H+  ++ VSL +QL  F ++  ++C S   S C+DM + +L +MGEIG
Sbjct: 129 ALEPSFLEERGIHSTITN-VSLSVQLRSFTESLPNLCGS--PSDCRDMIENALILMGEIG 188

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN  LF ++ ++EV  LVP VI  I SAI  L+ +G  T +VPGNFPIG    YL L
Sbjct: 189 GNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTL 248

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           +KTSN E YD   GCLKWLN FSEY+N+QLQ+EL  +R L PHV++IYADY+NA +RLF 
Sbjct: 249 YKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQ 308

Query: 433 APKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELI 492
            P  FGF++  +   C V G+   NF   CG      CDDPS++ ++DG+H+TEA Y LI
Sbjct: 309 EPAKFGFMNRPLPACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLI 368

Query: 493 AISVLEDIFTAPQF 505
           +  +L+  +  P F
Sbjct: 369 SEGLLKGPYAIPPF 372

BLAST of Carg18219 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.9e-84
Identity = 161/314 (51.27%), Postives = 204/314 (64.97%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFH PTGRFS+GR+I+DFIA  LG PLV P+        G     F KG+NFAVGGAT
Sbjct: 67  ETFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPF-------YGSQNANFEKGVNFAVGGAT 126

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL  S+ EERG+H  P   VSL +QL  FK++  ++C S   S C+DM + SL +MGEIG
Sbjct: 127 ALERSFLEERGIH-FPYTNVSLAVQLSSFKESLPNLCVS--PSDCRDMIENSLILMGEIG 186

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN   F  +NIEE+  LVPLVI+ I SAI  LI +G  T +VPG FP+GC   YL L
Sbjct: 187 GNDYNYAFFVGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSL 246

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           ++TSN E YD   GCLKWLN+FSEYH+EQLQ EL +++ L PHV++IYADY+N  +RL  
Sbjct: 247 YQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQ 306

Query: 433 APKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELI 492
            P  FGF+   +   C + G          G      CDDPSK+ SWDG+H+TEA Y L+
Sbjct: 307 EPAKFGFISRPLPACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLM 366

Query: 493 AISVLEDIFTAPQF 505
           A  +L+  +  P F
Sbjct: 367 AEGILKGPYAIPPF 370

BLAST of Carg18219 vs. ExPASy Swiss-Prot
Match: Q9ZQI3 (GDSL esterase/lipase At2g27360 OS=Arabidopsis thaliana OX=3702 GN=At2g27360 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 5.6e-84
Identity = 159/323 (49.23%), Postives = 206/323 (63.78%), Query Frame = 0

Query: 185 ETLFNRDRDTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGL 244
           E+ F    +TFFH P+GRFSDGR+I+DFIA  LG+P V P+        G     F KG+
Sbjct: 58  ESAFPPYGETFFHHPSGRFSDGRLIIDFIAEFLGIPHVPPF-------YGSKNGNFEKGV 117

Query: 245 NFAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKS 304
           NFAVGGATAL  S  EE+G H   S+ +SL  QL  FK++   +C SS S  C+DM + +
Sbjct: 118 NFAVGGATALECSVLEEKGTHCSQSN-ISLGNQLKSFKESLPYLCGSS-SPDCRDMIENA 177

Query: 305 LFIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIG 364
             ++GEIGGNDYN  LFD++NIEEV  LVPLVI  I SAI  L+++G  T +VPGNFP+G
Sbjct: 178 FILIGEIGGNDYNFPLFDRKNIEEVKELVPLVITTISSAISELVDMGARTFLVPGNFPLG 237

Query: 365 CVPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYF 424
           C   YL L++T N E Y+   GCL WLN FS YHNEQLQ ELK++R L PHV++IY DY+
Sbjct: 238 CSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVYHNEQLQAELKRLRNLYPHVNIIYGDYY 297

Query: 425 NAAMRLFNAPKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHL 484
           N  +RL   P  FG +D  +   C + G     F + CG      C DPSK+ +WDG+H+
Sbjct: 298 NTLLRLMQEPSKFGLMDRPLPACCGLGGPYNFTFSIKCGSKGVEYCSDPSKYVNWDGIHM 357

Query: 485 TEATYELIAISVLEDIFTAPQFS 506
           TEA Y+ I+  VL   +  P F+
Sbjct: 358 TEAAYKWISEGVLTGPYAIPPFN 371

BLAST of Carg18219 vs. ExPASy Swiss-Prot
Match: Q9FXJ2 (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana OX=3702 GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.2e-81
Identity = 155/314 (49.36%), Postives = 200/314 (63.69%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           + FFH PTGRFS+GR+I+DFIA  LGLPLV P+        G     F KG+NFAVGGAT
Sbjct: 70  ENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPF-------YGSHNANFEKGVNFAVGGAT 129

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL  S+ E+RG+H  P   VSL +QL+ FK++  S+C S   S C+DM + +L +MGEIG
Sbjct: 130 ALERSFLEDRGIH-FPYTNVSLGVQLNSFKESLPSICGS--PSDCRDMIENALILMGEIG 189

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN   F  + IEE+  L+PLVI  I SAI  LI +G  T +VPG FP+GC  +YL  
Sbjct: 190 GNDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTS 249

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
            +TSN E YD   GCLKWLN+F E H EQL+ EL +++ L PHV++IYADY+NA   L+ 
Sbjct: 250 HQTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQ 309

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPV--PCGLPSTIVCDDPSKHFSWDGLHLTEATYELI 492
            P  FGF++     C       N+ V   CG      CDDPSK+ +WDG+H+TEA Y L+
Sbjct: 310 EPAKFGFMNRPLSACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLM 369

Query: 493 AISVLEDIFTAPQF 505
           A  +L   +  P F
Sbjct: 370 AEGILNGPYAIPPF 373

BLAST of Carg18219 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.4e-81
Identity = 154/321 (47.98%), Postives = 201/321 (62.62%), Query Frame = 0

Query: 186 TLFNRDRDTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLN 245
           T F    +TFFH PTGR  DGR+I+DFIA  +GLP V PY        G     F KG+N
Sbjct: 57  TAFPPYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPY-------FGSKNRNFDKGVN 116

Query: 246 FAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSL 305
           FAV GATAL  S+ ++RG+   P   VSL +QL  FKK+  ++C S   S C+DM   +L
Sbjct: 117 FAVAGATALKSSFLKKRGIQ--PHTNVSLGVQLKSFKKSLPNLCGS--PSDCRDMIGNAL 176

Query: 306 FIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGC 365
            +MGEIGGNDYN   F+++ ++EV  LVP VI  I S I  LI +G  T +VPG FPIGC
Sbjct: 177 ILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGC 236

Query: 366 VPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFN 425
             +YL L+KTSN + YD   GCLKWLN+F EYH+E+L+ EL ++R L PHV++IYADY+N
Sbjct: 237 SVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYN 296

Query: 426 AAMRLFNAPKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLT 485
           + +R+F  P  FGF++      C + G    NF   CG      C DPSK+  WDG+H+T
Sbjct: 297 SLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMT 356

Query: 486 EATYELIAISVLEDIFTAPQF 505
           EA Y+ IA  +L   +  P F
Sbjct: 357 EAAYKWIADGILNGPYANPPF 366

BLAST of Carg18219 vs. ExPASy TrEMBL
Match: A0A6J1EFN7 (GDSL esterase/lipase At1g28570-like OS=Cucurbita moschata OX=3662 GN=LOC111433889 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 3.4e-177
Identity = 306/316 (96.84%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLG EGISAEEF KGLNFAVGGAT
Sbjct: 70  ETFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGEEGISAEEFRKGLNFAVGGAT 129

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG
Sbjct: 130 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 189

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAI  LIELGVDTIMVPGNFPIGCVPMYLKL
Sbjct: 190 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIRELIELGVDTIMVPGNFPIGCVPMYLKL 249

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQEL++I AL+PHVHLIYADYFNAAMRLFN
Sbjct: 250 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELERIGALHPHVHLIYADYFNAAMRLFN 309

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 492
           APKNFGF+DIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI
Sbjct: 310 APKNFGFVDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 369

Query: 493 SVLEDIFTAPQFSIFQ 509
           SVLEDIFTAPQFSIFQ
Sbjct: 370 SVLEDIFTAPQFSIFQ 385

BLAST of Carg18219 vs. ExPASy TrEMBL
Match: A0A6J1KR60 (GDSL esterase/lipase At1g28570-like OS=Cucurbita maxima OX=3661 GN=LOC111497512 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 1.2e-145
Identity = 249/316 (78.80%), Postives = 279/316 (88.29%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFHRPTGRFSDGRV+LDFIAT LGLPLVRPYR VGLGGEGISAEEFGKGLNFAVGGAT
Sbjct: 66  ETFFHRPTGRFSDGRVVLDFIATWLGLPLVRPYRRVGLGGEGISAEEFGKGLNFAVGGAT 125

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL+LS+FEERGVHNVPSHAVSL IQL W  KTYSSVCASSASSGC+D+FK SLFI+GEIG
Sbjct: 126 ALDLSFFEERGVHNVPSHAVSLGIQLHWINKTYSSVCASSASSGCRDIFKNSLFILGEIG 185

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN FL D R I EV SLVPLV+K+I SAI  LIELGV TIMVPGN P+GC+P+YL++
Sbjct: 186 GNDYNYFLLDNRTITEVTSLVPLVVKEISSAITELIELGVGTIMVPGNLPLGCLPIYLQV 245

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           + TSN   YD +NGCLKWLNQFS+YHNEQLQQEL++IRAL+PHVHLIYADYFNA MR+ N
Sbjct: 246 YATSNPSQYDPQNGCLKWLNQFSKYHNEQLQQELERIRALHPHVHLIYADYFNAVMRILN 305

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAI 492
           APK FGF ++ QVCCVD NR+  FP+PCGLPSTIVCDDPSK+ SWDGLHLTEA Y+LIA 
Sbjct: 306 APKKFGFTNLHQVCCVDENRSYYFPMPCGLPSTIVCDDPSKYVSWDGLHLTEAAYKLIAT 365

Query: 493 SVLEDIFTAPQFSIFQ 509
           S+++  FTAPQFSI Q
Sbjct: 366 SLMQASFTAPQFSILQ 381

BLAST of Carg18219 vs. ExPASy TrEMBL
Match: A0A1R3KBF3 (Lipase, GDSL OS=Corchorus olitorius OX=93759 GN=COLO4_09653 PE=3 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 1.2e-118
Identity = 231/496 (46.57%), Postives = 301/496 (60.69%), Query Frame = 0

Query: 43  MGEIGGNDYNYFFFDNRKHYRTHIFVPLVVKQIGSVIMELIELGVDTIMVPGNLPIGCLP 102
           MGEIGG+DYN+     R       +VP+VV  I S + ELI LG  T +VPGNLPIGCLP
Sbjct: 1   MGEIGGDDYNHPLMQGRGIEEVRGYVPIVVDTIVSAVEELIRLGAVTFVVPGNLPIGCLP 60

Query: 103 IYLQVYATSNRGQYEPQNGCLKWLNQFSKYHNEQLQQELKRIRALHPHVHLIYADYFNAL 162
           ++L  + T N+ +Y+P  GCL W+NQ S+YHN+ LQQ+L +IR L+PHV++IYADY+N  
Sbjct: 61  VFLTYFQTQNKEEYDPLTGCLTWMNQLSQYHNQMLQQKLDQIRNLYPHVNIIYADYYNTA 120

Query: 163 MDILNAPKNFGFTNLTKFVYAIETLFNRDRD----------------------------- 222
           +     P  FGF    K        +N D +                             
Sbjct: 121 IRFYQNPAEFGFKETLKACCGKGGPYNYDENNQCGDPPVRTCCDDPSTYVVWDGATDFTR 180

Query: 223 --------TFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLN 282
                   TFFH PTGR SDGR+++DF+    G P + PY     GG    +E F KG+N
Sbjct: 181 FYFPPYGKTFFHYPTGRCSDGRLVIDFV----GKPFLPPYLKSKNGG----SEHFQKGVN 240

Query: 283 FAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSL 342
           FAV GATA++  +F+ERG+  +    VSL I++ +F++   S+C  S+SS CK++ + SL
Sbjct: 241 FAVVGATAIDNGFFQERGI-EINYTNVSLGIEMGFFRELLPSLC--SSSSDCKELLQNSL 300

Query: 343 FIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGC 402
            +MGEIGGNDYN+ LF  R +EEV   VPLV++ I SAI  LIELG  T +VPGNFPIGC
Sbjct: 301 IVMGEIGGNDYNNPLFQGRKVEEVQEFVPLVMQTIASAINELIELGAVTFLVPGNFPIGC 360

Query: 403 VPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFN 462
            P YL  F+ SN E YD   GCL WLNQFSEYHN+ LQ+EL QIR L+P V++IY DY+N
Sbjct: 361 SPAYLTKFQGSNKEDYDPLTGCLTWLNQFSEYHNKLLQKELDQIRKLHPQVNIIYVDYYN 420

Query: 463 AAMRLFNAPKNFGFLDIEQVCCVDGNR-TINFPVPCGLPST-IVCDDPSKHFSWDGLHLT 500
            AMR + +P  +GF +  + CC  G     N  + CG       CDDPS + SWDG+H T
Sbjct: 421 IAMRFYQSPSQYGFKETIKACCGGGGAYNYNSSMACGSSEVRTSCDDPSSYISWDGVHYT 480

BLAST of Carg18219 vs. ExPASy TrEMBL
Match: A0A5D3CNP6 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008070 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.5e-116
Identity = 218/359 (60.72%), Postives = 266/359 (74.09%), Query Frame = 0

Query: 149 PHVHLIYADYFNALMDILNAPKNFGFTNLTKFVYAIETLFNRDRDTFFHRPTGRFSDGRV 208
           P V   Y   FN   D L+   N  +T    F       F    +TFFHRPTGRFSDGR+
Sbjct: 18  PSVFGCYTSIFN-FGDSLSDTGNLYYT--CSFPNPPNVCFFPYGETFFHRPTGRFSDGRL 77

Query: 209 ILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGATALNLSYFEERGVHNVP 268
           +LDF+A SLGLP V+PY+ V    +GI+AE+F KGLNFAVGGATAL+LS+FE+RG+ N+P
Sbjct: 78  VLDFLAMSLGLPPVQPYQGV---EKGITAEDFQKGLNFAVGGATALDLSFFEQRGIPNLP 137

Query: 269 SHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIGGNDYNDFLFDKRNIEE 328
               SL  Q + F ++YSS+C SS    CKD+FK SLFIMGEIGGNDYN   FD RNIEE
Sbjct: 138 PTVDSLGNQFNSFNQSYSSICTSSPPK-CKDIFKSSLFIMGEIGGNDYNYLFFDGRNIEE 197

Query: 329 VASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKLFK-TSNGEHYDSKNGC 388
           + SLV LV+ +I S I+ LIELGV+T+MVP N PIGC+P+YL+ +K TSN   +D +NGC
Sbjct: 198 LESLVLLVVNKIASVILELIELGVETLMVPSNLPIGCLPVYLEFYKTTSNESQFDPQNGC 257

Query: 389 LKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFNAPKNFGFLDIEQVCC 448
           LKWLNQFSEYHN+QLQ++LK+IR L+PHVHLIY DYFNAAMR++NAPK+FG ++  QVCC
Sbjct: 258 LKWLNQFSEYHNQQLQEQLKRIRVLHPHVHLIYVDYFNAAMRIYNAPKDFGLVEPLQVCC 317

Query: 449 VDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAISVLEDIFTAPQFSI 507
           VD N +  FP PCG   TIVCDDPSK+ SWDGLHLTEA YEL+A S++   FT PQFSI
Sbjct: 318 VDKNGSYYFPPPCGGAGTIVCDDPSKYASWDGLHLTEAAYELMATSIINGEFTFPQFSI 369

BLAST of Carg18219 vs. ExPASy TrEMBL
Match: A0A1S3AZJ0 (GDSL esterase/lipase At1g28570-like OS=Cucumis melo OX=3656 GN=LOC103484462 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.5e-116
Identity = 218/359 (60.72%), Postives = 266/359 (74.09%), Query Frame = 0

Query: 149 PHVHLIYADYFNALMDILNAPKNFGFTNLTKFVYAIETLFNRDRDTFFHRPTGRFSDGRV 208
           P V   Y   FN   D L+   N  +T    F       F    +TFFHRPTGRFSDGR+
Sbjct: 18  PSVFGCYTSIFN-FGDSLSDTGNLYYT--CSFPNPPNVCFFPYGETFFHRPTGRFSDGRL 77

Query: 209 ILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGATALNLSYFEERGVHNVP 268
           +LDF+A SLGLP V+PY+ V    +GI+AE+F KGLNFAVGGATAL+LS+FE+RG+ N+P
Sbjct: 78  VLDFLAMSLGLPPVQPYQGV---EKGITAEDFQKGLNFAVGGATALDLSFFEQRGIPNLP 137

Query: 269 SHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIGGNDYNDFLFDKRNIEE 328
               SL  Q + F ++YSS+C SS    CKD+FK SLFIMGEIGGNDYN   FD RNIEE
Sbjct: 138 PTVDSLGNQFNSFNQSYSSICTSSPPK-CKDIFKSSLFIMGEIGGNDYNYLFFDGRNIEE 197

Query: 329 VASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKLFK-TSNGEHYDSKNGC 388
           + SLV LV+ +I S I+ LIELGV+T+MVP N PIGC+P+YL+ +K TSN   +D +NGC
Sbjct: 198 LESLVLLVVNKIASVILELIELGVETLMVPSNLPIGCLPVYLEFYKTTSNESQFDPQNGC 257

Query: 389 LKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFNAPKNFGFLDIEQVCC 448
           LKWLNQFSEYHN+QLQ++LK+IR L+PHVHLIY DYFNAAMR++NAPK+FG ++  QVCC
Sbjct: 258 LKWLNQFSEYHNQQLQEQLKRIRVLHPHVHLIYVDYFNAAMRIYNAPKDFGLVEPLQVCC 317

Query: 449 VDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELIAISVLEDIFTAPQFSI 507
           VD N +  FP PCG   TIVCDDPSK+ SWDGLHLTEA YEL+A S++   FT PQFSI
Sbjct: 318 VDKNGSYYFPPPCGGAGTIVCDDPSKYASWDGLHLTEAAYELMATSIINGEFTFPQFSI 369

BLAST of Carg18219 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 1.0e-85
Identity = 159/314 (50.64%), Postives = 209/314 (66.56%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFH PTGR+SDGR+I+DFIA  LG PLV P+        G     F KG+NFAV GAT
Sbjct: 69  ETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPF-------YGCQNANFKKGVNFAVAGAT 128

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL  S+ EERG+H+  ++ VSL +QL  F ++  ++C S   S C+DM + +L +MGEIG
Sbjct: 129 ALEPSFLEERGIHSTITN-VSLSVQLRSFTESLPNLCGS--PSDCRDMIENALILMGEIG 188

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN  LF ++ ++EV  LVP VI  I SAI  L+ +G  T +VPGNFPIG    YL L
Sbjct: 189 GNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTL 248

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           +KTSN E YD   GCLKWLN FSEY+N+QLQ+EL  +R L PHV++IYADY+NA +RLF 
Sbjct: 249 YKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQ 308

Query: 433 APKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELI 492
            P  FGF++  +   C V G+   NF   CG      CDDPS++ ++DG+H+TEA Y LI
Sbjct: 309 EPAKFGFMNRPLPACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLI 368

Query: 493 AISVLEDIFTAPQF 505
           +  +L+  +  P F
Sbjct: 369 SEGLLKGPYAIPPF 372

BLAST of Carg18219 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 314.7 bits (805), Expect = 1.4e-85
Identity = 161/314 (51.27%), Postives = 204/314 (64.97%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           +TFFH PTGRFS+GR+I+DFIA  LG PLV P+        G     F KG+NFAVGGAT
Sbjct: 67  ETFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPF-------YGSQNANFEKGVNFAVGGAT 126

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL  S+ EERG+H  P   VSL +QL  FK++  ++C S   S C+DM + SL +MGEIG
Sbjct: 127 ALERSFLEERGIH-FPYTNVSLAVQLSSFKESLPNLCVS--PSDCRDMIENSLILMGEIG 186

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN   F  +NIEE+  LVPLVI+ I SAI  LI +G  T +VPG FP+GC   YL L
Sbjct: 187 GNDYNYAFFVGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSL 246

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
           ++TSN E YD   GCLKWLN+FSEYH+EQLQ EL +++ L PHV++IYADY+N  +RL  
Sbjct: 247 YQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQ 306

Query: 433 APKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLTEATYELI 492
            P  FGF+   +   C + G          G      CDDPSK+ SWDG+H+TEA Y L+
Sbjct: 307 EPAKFGFISRPLPACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLM 366

Query: 493 AISVLEDIFTAPQF 505
           A  +L+  +  P F
Sbjct: 367 AEGILKGPYAIPPF 370

BLAST of Carg18219 vs. TAIR 10
Match: AT2G27360.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 4.0e-85
Identity = 159/323 (49.23%), Postives = 206/323 (63.78%), Query Frame = 0

Query: 185 ETLFNRDRDTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGL 244
           E+ F    +TFFH P+GRFSDGR+I+DFIA  LG+P V P+        G     F KG+
Sbjct: 58  ESAFPPYGETFFHHPSGRFSDGRLIIDFIAEFLGIPHVPPF-------YGSKNGNFEKGV 117

Query: 245 NFAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKS 304
           NFAVGGATAL  S  EE+G H   S+ +SL  QL  FK++   +C SS S  C+DM + +
Sbjct: 118 NFAVGGATALECSVLEEKGTHCSQSN-ISLGNQLKSFKESLPYLCGSS-SPDCRDMIENA 177

Query: 305 LFIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIG 364
             ++GEIGGNDYN  LFD++NIEEV  LVPLVI  I SAI  L+++G  T +VPGNFP+G
Sbjct: 178 FILIGEIGGNDYNFPLFDRKNIEEVKELVPLVITTISSAISELVDMGARTFLVPGNFPLG 237

Query: 365 CVPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYF 424
           C   YL L++T N E Y+   GCL WLN FS YHNEQLQ ELK++R L PHV++IY DY+
Sbjct: 238 CSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVYHNEQLQAELKRLRNLYPHVNIIYGDYY 297

Query: 425 NAAMRLFNAPKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHL 484
           N  +RL   P  FG +D  +   C + G     F + CG      C DPSK+ +WDG+H+
Sbjct: 298 NTLLRLMQEPSKFGLMDRPLPACCGLGGPYNFTFSIKCGSKGVEYCSDPSKYVNWDGIHM 357

Query: 485 TEATYELIAISVLEDIFTAPQFS 506
           TEA Y+ I+  VL   +  P F+
Sbjct: 358 TEAAYKWISEGVLTGPYAIPPFN 371

BLAST of Carg18219 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 8.3e-83
Identity = 155/314 (49.36%), Postives = 200/314 (63.69%), Query Frame = 0

Query: 193 DTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLNFAVGGAT 252
           + FFH PTGRFS+GR+I+DFIA  LGLPLV P+        G     F KG+NFAVGGAT
Sbjct: 70  ENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPF-------YGSHNANFEKGVNFAVGGAT 129

Query: 253 ALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSLFIMGEIG 312
           AL  S+ E+RG+H  P   VSL +QL+ FK++  S+C S   S C+DM + +L +MGEIG
Sbjct: 130 ALERSFLEDRGIH-FPYTNVSLGVQLNSFKESLPSICGS--PSDCRDMIENALILMGEIG 189

Query: 313 GNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGCVPMYLKL 372
           GNDYN   F  + IEE+  L+PLVI  I SAI  LI +G  T +VPG FP+GC  +YL  
Sbjct: 190 GNDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTS 249

Query: 373 FKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFNAAMRLFN 432
            +TSN E YD   GCLKWLN+F E H EQL+ EL +++ L PHV++IYADY+NA   L+ 
Sbjct: 250 HQTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQ 309

Query: 433 APKNFGFLDIEQVCCVDGNRTINFPV--PCGLPSTIVCDDPSKHFSWDGLHLTEATYELI 492
            P  FGF++     C       N+ V   CG      CDDPSK+ +WDG+H+TEA Y L+
Sbjct: 310 EPAKFGFMNRPLSACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLM 369

Query: 493 AISVLEDIFTAPQF 505
           A  +L   +  P F
Sbjct: 370 AEGILNGPYAIPPF 373

BLAST of Carg18219 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 2.4e-82
Identity = 154/321 (47.98%), Postives = 201/321 (62.62%), Query Frame = 0

Query: 186 TLFNRDRDTFFHRPTGRFSDGRVILDFIATSLGLPLVRPYRSVGLGGEGISAEEFGKGLN 245
           T F    +TFFH PTGR  DGR+I+DFIA  +GLP V PY        G     F KG+N
Sbjct: 57  TAFPPYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPY-------FGSKNRNFDKGVN 116

Query: 246 FAVGGATALNLSYFEERGVHNVPSHAVSLEIQLDWFKKTYSSVCASSASSGCKDMFKKSL 305
           FAV GATAL  S+ ++RG+   P   VSL +QL  FKK+  ++C S   S C+DM   +L
Sbjct: 117 FAVAGATALKSSFLKKRGIQ--PHTNVSLGVQLKSFKKSLPNLCGS--PSDCRDMIGNAL 176

Query: 306 FIMGEIGGNDYNDFLFDKRNIEEVASLVPLVIKQIGSAIMGLIELGVDTIMVPGNFPIGC 365
            +MGEIGGNDYN   F+++ ++EV  LVP VI  I S I  LI +G  T +VPG FPIGC
Sbjct: 177 ILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGC 236

Query: 366 VPMYLKLFKTSNGEHYDSKNGCLKWLNQFSEYHNEQLQQELKQIRALNPHVHLIYADYFN 425
             +YL L+KTSN + YD   GCLKWLN+F EYH+E+L+ EL ++R L PHV++IYADY+N
Sbjct: 237 SVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYN 296

Query: 426 AAMRLFNAPKNFGFLD--IEQVCCVDGNRTINFPVPCGLPSTIVCDDPSKHFSWDGLHLT 485
           + +R+F  P  FGF++      C + G    NF   CG      C DPSK+  WDG+H+T
Sbjct: 297 SLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMT 356

Query: 486 EATYELIAISVLEDIFTAPQF 505
           EA Y+ IA  +L   +  P F
Sbjct: 357 EAAYKWIADGILNGPYANPPF 366