Carg18201 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18201
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Plant invertase/pectin methylesterase inhibitor
Location	Carg_Chr11: 903803 .. 904276 (+)
RNA-Seq Expression	Carg18201
Synteny	Carg18201

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCAAGAAACATGCAAGAAATGTGCAGCCACCACTCCAAACCTCAGCTACGCCTTCTGTGTTTCGTCTTTAGAATCCGATGATCGGAGCCGCTCGGCAAGTCTCCACAGACTTGGGCTCATCTCCATGGATCTACTGCGCCACAACTTAACCAGCACACGACGTGAAGTCAAGAAGCTTTTGAGGAACAAGACATTGGATGGGTTCGTTAAGGCGCGTTTAGATGATTGTTTGGAGCTTTATTCTGATGCGATTCCGACGCTGAAAGAATGTAAAAGGGAGTATAAACAGAAGCGTTTTAGTGATGCAAATGTGAAGGTTAGTTCAATCATGGAAGCTCCTTCAACTTGCGAAGATGGGTTTCATGAAAAACAAGGCGTGGTTTCACCGTTGACTAAGAATAATAAGAATGTTTTTCAGTTAGCTGCGTTGACTCTCTCCATCGTCAACATGAATATGCATCTTCAATGA

mRNA sequence

ATGGTTCAAGAAACATGCAAGAAATGTGCAGCCACCACTCCAAACCTCAGCTACGCCTTCTGTGTTTCGTCTTTAGAATCCGATGATCGGAGCCGCTCGGCAAGTCTCCACAGACTTGGGCTCATCTCCATGGATCTACTGCGCCACAACTTAACCAGCACACGACGTGAAGTCAAGAAGCTTTTGAGGAACAAGACATTGGATGGGTTCGTTAAGGCGCGTTTAGATGATTGTTTGGAGCTTTATTCTGATGCGATTCCGACGCTGAAAGAATGTAAAAGGGAGTATAAACAGAAGCGTTTTAGTGATGCAAATGTGAAGGTTAGTTCAATCATGGAAGCTCCTTCAACTTGCGAAGATGGGTTTCATGAAAAACAAGGCGTGGTTTCACCGTTGACTAAGAATAATAAGAATGTTTTTCAGTTAGCTGCGTTGACTCTCTCCATCGTCAACATGAATATGCATCTTCAATGA

Coding sequence (CDS)

ATGGTTCAAGAAACATGCAAGAAATGTGCAGCCACCACTCCAAACCTCAGCTACGCCTTCTGTGTTTCGTCTTTAGAATCCGATGATCGGAGCCGCTCGGCAAGTCTCCACAGACTTGGGCTCATCTCCATGGATCTACTGCGCCACAACTTAACCAGCACACGACGTGAAGTCAAGAAGCTTTTGAGGAACAAGACATTGGATGGGTTCGTTAAGGCGCGTTTAGATGATTGTTTGGAGCTTTATTCTGATGCGATTCCGACGCTGAAAGAATGTAAAAGGGAGTATAAACAGAAGCGTTTTAGTGATGCAAATGTGAAGGTTAGTTCAATCATGGAAGCTCCTTCAACTTGCGAAGATGGGTTTCATGAAAAACAAGGCGTGGTTTCACCGTTGACTAAGAATAATAAGAATGTTTTTCAGTTAGCTGCGTTGACTCTCTCCATCGTCAACATGAATATGCATCTTCAATGA

Protein sequence

MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ

Homology

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BLAST of Carg18201 vs. NCBI nr
Match: KAG6587552.1 (hypothetical protein SDJN03_16117, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021528.1 hypothetical protein SDJN02_15253, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 308.5 bits (789), Expect = 3.2e-80
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED
Sbjct: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ
Sbjct: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 157

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BLAST of Carg18201 vs. NCBI nr
Match: XP_023531537.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 306.2 bits (783), Expect = 1.6e-79
Identity = 155/157 (98.73%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA+LHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSANLHRLGLISMDLLRHNLTSTRREVKK 60

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRF+DANVKVSSIMEAPSTCED
Sbjct: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFNDANVKVSSIMEAPSTCED 120

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ
Sbjct: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 157

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BLAST of Carg18201 vs. NCBI nr
Match: XP_022933437.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 302.8 bits (774), Expect = 1.8e-78
Identity = 155/157 (98.73%), Postives = 155/157 (98.73%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 30  MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 89

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNKTLDGFVKA LDDCLELYSDAIPTLKECKREYKQKRFS ANVKVSSIMEAPSTCED
Sbjct: 90  LLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSGANVKVSSIMEAPSTCED 149

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ
Sbjct: 150 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 186

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BLAST of Carg18201 vs. NCBI nr
Match: XP_022973011.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 300.1 bits (767), Expect = 1.2e-77
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           MVQETCKKCAATTPNLSY+FCVSSLESDDRSRSA+LHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 30  MVQETCKKCAATTPNLSYSFCVSSLESDDRSRSANLHRLGLISMDLLRHNLTSTRREVKK 89

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNKTLDGFVK RLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED
Sbjct: 90  LLRNKTLDGFVKVRLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 149

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GFHEKQGV SPLTK+NKNVFQLAALTLSIVNMNMHLQ
Sbjct: 150 GFHEKQGVGSPLTKSNKNVFQLAALTLSIVNMNMHLQ 186

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BLAST of Carg18201 vs. NCBI nr
Match: XP_038880152.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 250.8 bits (639), Expect = 8.0e-63
Identity = 120/157 (76.43%), Postives = 141/157 (89.81%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           ++QETCKK A ++PN+SY FCV+S ESD+RSRSA+LH+LGLISMDL+RHN+TSTRRE+KK
Sbjct: 32  IIQETCKKSAESSPNISYKFCVASFESDNRSRSANLHKLGLISMDLMRHNITSTRREIKK 91

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNK L+ F+K  L DCLELYSDA+PTLKE KREYK+K++ DANVKVSSIMEAP+TCED
Sbjct: 92  LLRNKKLEEFIKGCLKDCLELYSDAVPTLKEAKREYKEKKYKDANVKVSSIMEAPTTCED 151

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GF EKQG +SPLT NN N FQLAALTLSI+NMNMHLQ
Sbjct: 152 GFQEKQGTISPLTNNNSNAFQLAALTLSIINMNMHLQ 188

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BLAST of Carg18201 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.5e-27
Identity = 64/152 (42.11%), Postives = 105/152 (69.08%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           +VQ TCKK A  +PN++Y FCV SL +D +S +A L  LG+IS +L   + +  +  + +
Sbjct: 26  IVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIGR 85

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           +L++K +D  +K  L+DC+ LY+DA  +++E   ++K K ++ ANVK+S+ ++   TCED
Sbjct: 86  ILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCED 145

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 153
           GF EK+G+VSP+TK NK+  QL A++L+I  +
Sbjct: 146 GFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

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BLAST of Carg18201 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.5e-27
Identity = 64/152 (42.11%), Postives = 103/152 (67.76%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           +VQ TCKK A  +PN++Y FCV SL +D +S SA L  LG+IS +L     +  +  + +
Sbjct: 17  IVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKIQTFIGR 76

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           +L++K +D  +K  L+DC+ LY+DA  +++E   ++K K ++ ANVK+S+ ++   TCED
Sbjct: 77  ILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCED 136

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 153
           GF EK+G+ SP+TK NK+  QL A++L+I  +
Sbjct: 137 GFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

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BLAST of Carg18201 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 7.2e-14
Identity = 50/153 (32.68%), Postives = 81/153 (52.94%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLISMDLLRHNLTSTRREVK 60
           ++Q++CKK  A  P LSY FCV+SL  D +S++A +L  L L S       +T+ +  V 
Sbjct: 23  LIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTKTAAAKITNLKGIVA 82

Query: 61  KLLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCE 120
           + L+++     V+  L  CL  Y+DA   L       K + +  AN+ +S+ ++ P  CE
Sbjct: 83  QDLKDQRYQDIVE-DLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVPGNCE 142

Query: 121 DGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 153
           D F E +   SP+T  N  +F+   + L+  NM
Sbjct: 143 DDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

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BLAST of Carg18201 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.0e-07
Identity = 39/132 (29.55%), Postives = 65/132 (49.24%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           +++ TCK     T N  Y FCVS+L+SD RS +A    L  I + +   N TST   +  
Sbjct: 30  IIESTCK-----TTNY-YKFCVSALKSDPRSPTADTKGLASIMVGVGMTNATSTANYIAG 89

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
            L     D  +K  L DC E Y+ A  +L+   ++   + +  A++ V +  + P+ C +
Sbjct: 90  NLSATVKDTVLKKVLQDCSEKYALAADSLRLTIQDLDDEAYDYASMHVLAAQDYPNVCRN 149

Query: 121 GFHEKQGVVSPL 133
            F   +G+  P+
Sbjct: 150 IFRRVKGLAYPV 155

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BLAST of Carg18201 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.9e-06
Identity = 38/135 (28.15%), Postives = 66/135 (48.89%), Query Frame = 0


Query: 19  AFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKARLDDC 78
           +FC S ++S+ ++  A L  L  I+    + + +   R+++ L++  T +  +K     C
Sbjct: 41  SFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTAT-NPTMKKAYTSC 100

Query: 79  LELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKN 138
           ++ Y  AI +L + K+          N+KVS+ ME PSTCE    + +  V P    N  
Sbjct: 101 VQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFK--VDPSAVKNSG 160

Query: 139 VFQ-LAALTLSIVNM 153
            FQ +  + L I NM
Sbjct: 161 DFQNICGIVLVISNM 172

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BLAST of Carg18201 vs. ExPASy TrEMBL
Match: A0A6J1EZS0 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 8.6e-79
Identity = 155/157 (98.73%), Postives = 155/157 (98.73%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 30  MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 89

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNKTLDGFVKA LDDCLELYSDAIPTLKECKREYKQKRFS ANVKVSSIMEAPSTCED
Sbjct: 90  LLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSGANVKVSSIMEAPSTCED 149

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ
Sbjct: 150 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 186

[Close]

BLAST of Carg18201 vs. ExPASy TrEMBL
Match: A0A6J1I7I4 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 5.6e-78
Identity = 152/157 (96.82%), Postives = 155/157 (98.73%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           MVQETCKKCAATTPNLSY+FCVSSLESDDRSRSA+LHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 30  MVQETCKKCAATTPNLSYSFCVSSLESDDRSRSANLHRLGLISMDLLRHNLTSTRREVKK 89

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNKTLDGFVK RLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED
Sbjct: 90  LLRNKTLDGFVKVRLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 149

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GFHEKQGV SPLTK+NKNVFQLAALTLSIVNMNMHLQ
Sbjct: 150 GFHEKQGVGSPLTKSNKNVFQLAALTLSIVNMNMHLQ 186

[Close]

BLAST of Carg18201 vs. ExPASy TrEMBL
Match: A0A6J1C4E5 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.9e-62
Identity = 120/157 (76.43%), Postives = 143/157 (91.08%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           ++QETCK+ A +TPNLSY FCV+SLESDDRSR A LH+LGLI+MDL+RHN+TSTRR+++K
Sbjct: 55  IIQETCKRSADSTPNLSYNFCVASLESDDRSRCADLHKLGLIAMDLMRHNITSTRRDIRK 114

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNK LD FVKARLDDCLELYSDA+P LKE KR YK+K++ DANVKVSS+MEAPSTCED
Sbjct: 115 LLRNKKLDQFVKARLDDCLELYSDAVPILKEAKRCYKEKKYVDANVKVSSVMEAPSTCED 174

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 158
           GF +K+G+VSPLT+ NKNVF+LAALTLSI+NMN HLQ
Sbjct: 175 GFRDKEGLVSPLTEKNKNVFRLAALTLSIINMNTHLQ 211

[Close]

BLAST of Carg18201 vs. ExPASy TrEMBL
Match: A0A0A0LUJ3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.4e-60
Identity = 115/155 (74.19%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           ++QETCKK AA+TPNL+Y FCV+SLESD RSR A+LH+LGLISMDLLRHN+TSTRRE+KK
Sbjct: 31  IIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKK 90

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LLRNK ++ F+K  L DC+ELYSDA+PTLKE KREYK + + DAN+KVSSIMEAP+TCE+
Sbjct: 91  LLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCEN 150

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMH 156
           GF EK+G++SPLTKNN +VFQLAALTLSI+NMN+H
Sbjct: 151 GFKEKEGIISPLTKNNSDVFQLAALTLSIINMNLH 185

[Close]

BLAST of Carg18201 vs. ExPASy TrEMBL
Match: A0A2P6SEF8 (Putative pectinesterase inhibitor domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0344051 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 7.4e-46
Identity = 93/152 (61.18%), Postives = 119/152 (78.29%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60
           ++ ETCKKCA   PNLSY FCV+SL++   S  A L RLGLISM L++HN+T+TR  +K 
Sbjct: 29  LISETCKKCARNDPNLSYKFCVTSLQAAPNSHRADLRRLGLISMKLVQHNVTNTRHFIKH 88

Query: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120
           LL+NK LD FV+ARLDDCLELY+DAIPT+K+  + YK+K   DAN++VSSI++A +TCED
Sbjct: 89  LLKNKKLDKFVRARLDDCLELYTDAIPTIKQAIKNYKKKHNKDANIEVSSIVDASTTCED 148

Query: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 153
           GF E+ G+VSPLTK NK  FQL  + LSI+NM
Sbjct: 149 GFEERPGLVSPLTKRNKETFQLCIIALSIINM 180

[Close]

BLAST of Carg18201 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 1.8e-20
Identity = 58/142 (40.85%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLISMDLLRHNLTSTRREVK 60
           ++Q+ CKK A   P + Y FCV SLE + +S++A SL RL ++S        TS +  V 
Sbjct: 23  LIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLSTKNAVSKTTSMKGIVD 82

Query: 61  KLLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCE 120
           K+L+    + + +  L DCLELYSDA  +LKE     K + +  ANV +S+ M AP +CE
Sbjct: 83  KILKENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSRDYKTANVVISAAMGAPPSCE 142

Query: 121 DGFHE-KQGVVSPLTKNNKNVF 141
            GF E K+ + SP TK+N  +F
Sbjct: 143 IGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Carg18201 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 5.2e-20
Identity = 52/153 (33.99%), Postives = 88/153 (57.52%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLISMDLLRHNLTSTRREVK 60
           +++ +CKK  AT+P   Y  CV+SLE++ ++++A  L  L + S        T+ +  V 
Sbjct: 23  LIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLVMASTKNAVTKATTLKGTVD 82

Query: 61  KLLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCE 120
           K+++ K ++      L DCL+LY+DAI +L E     K + +      +S+ M+ PSTCE
Sbjct: 83  KIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRNYPTVKTVLSAAMDTPSTCE 142

Query: 121 DGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 153
            GF E++   SP+TK N N++Q+  + L+  NM
Sbjct: 143 TGFKERK-APSPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of Carg18201 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.4e-17
Identity = 60/165 (36.36%), Postives = 83/165 (50.30%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRS--RSASLHRLGLISMDLLRHNLTSTRREV 60
           M+ +TCK CAA +    Y FCVSSL +   +     +L  L L+ M     N T+T   +
Sbjct: 25  MIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSSLALVPMLQALDNATATASTI 84

Query: 61  KKLLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREY-KQKRFSDANVKVSSIMEAPST 120
           ++LL +     F  A L DCLELY DA   L+E  R +  +K     NV VS+ ME+  T
Sbjct: 85  QQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFITRKELGTVNVMVSAAMESAVT 144

Query: 121 CEDGFHEKQ-------GV---VSPLTKNNKNVFQLAALTLSIVNM 153
           CE+GF E+        GV    SP+   N  +F+   + L I NM
Sbjct: 145 CENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQIALCIFNM 189

[Close]

BLAST of Carg18201 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-16
Identity = 52/154 (33.77%), Postives = 85/154 (55.19%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLISMDLLRHNLTSTRREVK 60
           +++++CKK     P L Y FCV SLE + +S++A SL  L  +S        TS +  V 
Sbjct: 23  LIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLVFVSTKNAVSKTTSLKGMVD 82

Query: 61  KLLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCE 120
           K+L+    +  V+  L DCLELY++AI +L +     K + +  A + +S+ M+AP +CE
Sbjct: 83  KILKEDKYE--VERPLLDCLELYTEAIDSLNQSLDTVKSRDYKTATMLMSAAMDAPGSCE 142

Query: 121 DGF-HEKQGVVSPLTKNNKNVFQLAALTLSIVNM 153
             F   K+ V SP TK N  +F +  + ++  +M
Sbjct: 143 TKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of Carg18201 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 5.1e-15
Identity = 50/153 (32.68%), Postives = 81/153 (52.94%), Query Frame = 0


Query: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLISMDLLRHNLTSTRREVK 60
           ++Q++CKK  A  P LSY FCV+SL  D +S++A +L  L L S       +T+ +  V 
Sbjct: 23  LIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTKTAAAKITNLKGIVA 82

Query: 61  KLLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCE 120
           + L+++     V+  L  CL  Y+DA   L       K + +  AN+ +S+ ++ P  CE
Sbjct: 83  QDLKDQRYQDIVE-DLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVPGNCE 142

Query: 121 DGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 153
           D F E +   SP+T  N  +F+   + L+  NM
Sbjct: 143 DDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

The following BLAST results are available for this feature:

BLAST of Carg18201 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6587552.1	3.2e-80	100.00	hypothetical protein SDJN03_16117, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023531537.1	1.6e-79	98.73	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
XP_022933437.1	1.8e-78	98.73	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_022973011.1	1.2e-77	96.82	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_038880152.1	8.0e-63	76.43	putative invertase inhibitor [Benincasa hispida]	[more]

BLAST of Carg18201 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	1.5e-27	42.11	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	2.5e-27	42.11	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	7.2e-14	32.68	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
O49603	5.0e-07	29.55	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702...	[more]
Q9LUV1	1.9e-06	28.15	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Carg18201 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EZS0	8.6e-79	98.73	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 ...	[more]
A0A6J1I7I4	5.6e-78	96.82	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV...	[more]
A0A6J1C4E5	1.9e-62	76.43	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4...	[more]
A0A0A0LUJ3	1.4e-60	74.19	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 S...	[more]
A0A2P6SEF8	7.4e-46	61.18	Putative pectinesterase inhibitor domain-containing protein OS=Rosa chinensis OX...	[more]

BLAST of Carg18201 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46970.1	1.8e-20	40.85	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	5.2e-20	33.99	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	2.4e-17	36.36	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	1.6e-16	33.77	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	5.1e-15	32.68	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 44..64
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 2..152
None	No IPR available	PANTHER	PTHR31707:SF314	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR	coord: 2..152
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 1..149 e-value: 8.8E-27 score: 105.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 2..149 e-value: 9.4E-30 score: 104.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..152 e-value: 6.3E-38 score: 127.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 1..153 e-value: 3.3E-38 score: 133.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 2..152
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 6..152 e-value: 3.70257E-50 score: 155.986

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18201-RA	Carg18201-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity