Carg18196 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18196
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein of unknown function (DUF962)
Location	Carg_Chr11: 876574 .. 878253 (+)
RNA-Seq Expression	Carg18196
Synteny	Carg18196

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATTGTCTAACCCTCATAATCTATTCGGTTATCTCCCTTTGCACGCCTTCCCTCTTCTGAGAACATTTTCCAGCAATCGTTTCGTATTGGGGTTGTCTATTTTCCCCCAAAATTCCATGGGGAAGACTGCATTTTTTGATCTGGAAAGGCACTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATGTCCTGTTTGTTTGGCCGATTTTCTTCACCACTCTCATGTATTTGTATTTCACCCCTTCTTTCTATACGATTCCTAAATCCCCTTGTGGGTTTGATCATGGCCTAGTTTTGAACTTCGGATTTCTTTTCACTTTAATCTATGCTGCATCTTATGTGATCTTCGATAAGAGAGCAGGGTCCATGGCCGCTTTGCTTTGTTTCGTCTGTTGGGTTGGAGCAAGCTTCATCTCCAATAGACTTGGTTATTCCCAGACTTGGAAGGTAAATTCCCACTTTCTCTTCTTCAATTTAACTCCAGGTTCTTGTAGTAAATTTGTGTGTTTATTACTATTGAGTGCACTGAAGTTTCTTCCTTGAAATTTCCTTGATGGGTTTTTATTTGCCTCTGCTTACAATCACTATAAGCTTGTAATCTAATCTAATCTGGTTGGATTTGGACGATGTGTTAAAGTTTTGACATCTAAGTGGCAGCAAGTTGGGGCCATGTCAAATGGCACTTGGGGTTGATATTGCTGCGCATGGAAGGAGCTTTTATTGCCTGACCAGTTGGTTGATAATCATAATAGTTCTTTATTTATGACAGATCCTTAGCCTCACCAAAATTTCAAGGCCATTTAGTCCTTGAGAGTTTGAAAACGATTTCAGGACATTAGGAATGCTGCATACTTCATACGCTAACCTAATTCATTTTTTGTTGTATATCAGGTGGTACTGGCTGCTCAGCTGTTCTGTTGGACAAACCAGTTTATAGGCCATGGAGTGTTTGAGGTTAGACAGATCACTCATCTTGACAAATTGATTGCGTCTGTTCTTGAATCTTAATTTAATTTGCATTTTTCATTAGAAACGTGCACCGGCTTTGTTGGACAATCTTGCACAAGCCTTTCTAATGGCTCCATTCTTTGTGTTTTTGGAGGTATTCTTTGTCTGGTTTTCTCATCTTCTGAGGGTTGTTTATTAGTGTGGATAATGAAGTAGCTTCGTTGTTCCTTTTGGTTGGTTGCAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGCTTTAGTGCAAGTGTGCAAGCAAAGATAGAAGCAGATATAAAAGAGTGGAAAGAAAAGAAGGAGAAGCTCTCATAGGTTGAATTGCCTGGACTTTGATTAAAAGAAAGGTCTGATCTGGGTTGCCCTTAGTAAAAATACTGTGAAAACTAACTACTTCATATGAACATTTCAAAATCTATCAATGATATTATTGTACATGTTCTGCTTTCCAACCTTGATTTGCTATTTTACTAGTGTGTCTGTCAGAGTACACTAAAATGAATGAGGACAACATGGGGTGTGATGACTTACTTGTTTACTAGATCCTTCAAATGCATGCTCTAACAACTTAAGCCCAACGCCCGTAGATATTTTCCTCTCTTGCCTTTTCTTTTTAGGCTTCTCCTCAAGGTTTTAAAACGCCTTTGCTAGGGAGGTTTCTGCACCATTTA

mRNA sequence

CAATTGTCTAACCCTCATAATCTATTCGGTTATCTCCCTTTGCACGCCTTCCCTCTTCTGAGAACATTTTCCAGCAATCGTTTCGTATTGGGGTTGTCTATTTTCCCCCAAAATTCCATGGGGAAGACTGCATTTTTTGATCTGGAAAGGCACTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATGTCCTGTTTGTTTGGCCGATTTTCTTCACCACTCTCATGTATTTGTATTTCACCCCTTCTTTCTATACGATTCCTAAATCCCCTTGTGGGTTTGATCATGGCCTAGTTTTGAACTTCGGATTTCTTTTCACTTTAATCTATGCTGCATCTTATGTGATCTTCGATAAGAGAGCAGGGTCCATGGCCGCTTTGCTTTGTTTCGTCTGTTGGGTTGGAGCAAGCTTCATCTCCAATAGACTTGGTTATTCCCAGACTTGGAAGGTGGTACTGGCTGCTCAGCTGTTCTGTTGGACAAACCAGTTTATAGGCCATGGAGTGTTTGAGAAACGTGCACCGGCTTTGTTGGACAATCTTGCACAAGCCTTTCTAATGGCTCCATTCTTTGTGTTTTTGGAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGCTTTAGTGCAAGTGTGCAAGCAAAGATAGAAGCAGATATAAAAGAGTGGAAAGAAAAGAAGGAGAAGCTCTCATAGGTTGAATTGCCTGGACTTTGATTAAAAGAAAGGTCTGATCTGGGTTGCCCTTAGTAAAAATACTGTGAAAACTAACTACTTCATATGAACATTTCAAAATCTATCAATGATATTATTGTACATGTTCTGCTTTCCAACCTTGATTTGCTATTTTACTAGTGTGTCTGTCAGAGTACACTAAAATGAATGAGGACAACATGGGGTGTGATGACTTACTTGTTTACTAGATCCTTCAAATGCATGCTCTAACAACTTAAGCCCAACGCCCGTAGATATTTTCCTCTCTTGCCTTTTCTTTTTAGGCTTCTCCTCAAGGTTTTAAAACGCCTTTGCTAGGGAGGTTTCTGCACCATTTA

Coding sequence (CDS)

ATGGGGAAGACTGCATTTTTTGATCTGGAAAGGCACTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATGTCCTGTTTGTTTGGCCGATTTTCTTCACCACTCTCATGTATTTGTATTTCACCCCTTCTTTCTATACGATTCCTAAATCCCCTTGTGGGTTTGATCATGGCCTAGTTTTGAACTTCGGATTTCTTTTCACTTTAATCTATGCTGCATCTTATGTGATCTTCGATAAGAGAGCAGGGTCCATGGCCGCTTTGCTTTGTTTCGTCTGTTGGGTTGGAGCAAGCTTCATCTCCAATAGACTTGGTTATTCCCAGACTTGGAAGGTGGTACTGGCTGCTCAGCTGTTCTGTTGGACAAACCAGTTTATAGGCCATGGAGTGTTTGAGAAACGTGCACCGGCTTTGTTGGACAATCTTGCACAAGCCTTTCTAATGGCTCCATTCTTTGTGTTTTTGGAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGCTTTAGTGCAAGTGTGCAAGCAAAGATAGAAGCAGATATAAAAGAGTGGAAAGAAAAGAAGGAGAAGCTCTCATAG

Protein sequence

MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGFDHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQAKIEADIKEWKEKKEKLS

Homology

[Close]

BLAST of Carg18196 vs. NCBI nr
Match: XP_022933403.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita moschata] >KAG7021523.1 SPAC16E8.02 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 406.8 bits (1044), Expect = 1.1e-109
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKIEADIKEWKEKKEKLS
Sbjct: 181 AKIEADIKEWKEKKEKLS 198

[Close]

BLAST of Carg18196 vs. NCBI nr
Match: XP_023531593.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.1 bits (1024), Expect = 2.3e-107
Identity = 192/195 (98.46%), Postives = 195/195 (100.00%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKTAFFDLERHFAFYGAYHSNP+NIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPINIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYVIFDK+AGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKE 196
           AKIEADIKEWKEKK+
Sbjct: 181 AKIEADIKEWKEKKK 195

[Close]

BLAST of Carg18196 vs. NCBI nr
Match: XP_022972978.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita maxima])

HSP 1 Score: 396.7 bits (1018), Expect = 1.1e-106
Identity = 192/198 (96.97%), Postives = 195/198 (98.48%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKTAFFDLERH+AFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTL YAASYVIFDK+AGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQ IGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFS+SVQ
Sbjct: 121 AQLFCWTNQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKIEADIKEWKE KEKLS
Sbjct: 181 AKIEADIKEWKEMKEKLS 198

[Close]

BLAST of Carg18196 vs. NCBI nr
Match: XP_038880038.1 (2-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida])

HSP 1 Score: 387.9 bits (995), Expect = 5.3e-104
Identity = 185/198 (93.43%), Postives = 195/198 (98.48%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGK+  FDLERHFAFYGAYHSNPVNIFIH+LFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYV+FDK+AGSMAALLCFVCWVGASFI+NRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKI+ADI+EWKEKK+KLS
Sbjct: 181 AKIKADIEEWKEKKKKLS 198

[Close]

BLAST of Carg18196 vs. NCBI nr
Match: XP_004138174.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus] >KGN63680.1 hypothetical protein Csa_014278 [Cucumis sativus])

HSP 1 Score: 380.6 bits (976), Expect = 8.5e-102
Identity = 182/198 (91.92%), Postives = 192/198 (96.97%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKT  FDLE+ FAFYGAYHSNP+NIFIHVLFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTL+YAA YV+FDKRAGSMAALLCFVCWVGASFI+N+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKI+ADI+EWKE KEKLS
Sbjct: 181 AKIKADIREWKETKEKLS 198

[Close]

BLAST of Carg18196 vs. ExPASy Swiss-Prot
Match: O13737 (2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mpo1 PE=3 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 3.2e-19
Identity = 68/181 (37.57%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 9   LERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGFDHGLVLNF 68
           L R ++FY AYHSNPVNI IH + +  +  T L+ L+      T+  S       L +N 
Sbjct: 4   LSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLH--NFVITLINSK------LQINV 63

Query: 69  GFLFTLIYAASYVIFDKRAGSM-AALLCFVCWVGASFISNRLGYSQTWKVVLAAQLFCWT 128
             L  L Y   YV  D   G + + +L    ++  S +      S   +      + CW 
Sbjct: 64  AHLVGLAYQIFYVTLDPLDGLLYSPVLYLFSYILPSKLFTIFSRSLVNRSAAVVHVICWI 123

Query: 129 NQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQAKIEADI 188
            QFIGHGVFEKR PALLDNL Q+  +AP F FLE         P+ G+  SV +KI A+I
Sbjct: 124 LQFIGHGVFEKRKPALLDNLIQSLFIAPLFAFLET-------GPFVGYYPSVVSKIRANI 169

[Close]

BLAST of Carg18196 vs. ExPASy Swiss-Prot
Match: P25338 (2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MPO1 PE=1 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 60/181 (33.15%), Postives = 86/181 (47.51%), Query Frame = 0


Query: 8   DLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGFDHGLVLN 67
           DL     FY  YH NP N+ IH +FV  I F+    L+    + +I          L   
Sbjct: 7   DLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLHRVKIYQSI---------SLTAV 66

Query: 68  FGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLAAQLFCWT 127
              LF++ Y   Y+     AG +  LL        + I +R+    T+K  L      W 
Sbjct: 67  LSVLFSIFYCLLYLPTGLLAGVLLLLL------NLALIDHRV--DLTFKQELGLFTIGWI 126

Query: 128 NQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQAKIEADI 187
            QF+GHGVFEKR PAL+DNL Q+ ++AP+F+  E L  L       GF   ++A +E D+
Sbjct: 127 FQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFLFKL-------GFMPRLKATLEHDL 163

Query: 188 K 189
           +
Sbjct: 187 E 163

[Close]

BLAST of Carg18196 vs. ExPASy TrEMBL
Match: A0A6J1F4N0 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita moschata OX=3662 GN=LOC111440831 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 5.3e-110
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKIEADIKEWKEKKEKLS
Sbjct: 181 AKIEADIKEWKEKKEKLS 198

[Close]

BLAST of Carg18196 vs. ExPASy TrEMBL
Match: A0A6J1IBP8 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita maxima OX=3661 GN=LOC111471498 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 5.5e-107
Identity = 192/198 (96.97%), Postives = 195/198 (98.48%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKTAFFDLERH+AFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTL YAASYVIFDK+AGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQ IGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFS+SVQ
Sbjct: 121 AQLFCWTNQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKIEADIKEWKE KEKLS
Sbjct: 181 AKIEADIKEWKEMKEKLS 198

[Close]

BLAST of Carg18196 vs. ExPASy TrEMBL
Match: A0A0A0LP54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.1e-102
Identity = 182/198 (91.92%), Postives = 192/198 (96.97%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKT  FDLE+ FAFYGAYHSNP+NIFIHVLFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTL+YAA YV+FDKRAGSMAALLCFVCWVGASFI+N+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKI+ADI+EWKE KEKLS
Sbjct: 181 AKIKADIREWKETKEKLS 198

[Close]

BLAST of Carg18196 vs. ExPASy TrEMBL
Match: A0A5A7UQ13 (Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002550 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.4e-102
Identity = 181/198 (91.41%), Postives = 192/198 (96.97%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKT  FDLE+ FAFYGAYHSNP+NIFIHVLFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGF FTL+YAA YV+FDKRAGSMAALLCFVCWVGAS+I+N+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKI+ADI+EWKEKKEKLS
Sbjct: 181 AKIKADIREWKEKKEKLS 198

[Close]

BLAST of Carg18196 vs. ExPASy TrEMBL
Match: A0A1S3BV50 (uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo OX=3656 GN=LOC103494003 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.4e-102
Identity = 181/198 (91.41%), Postives = 192/198 (96.97%), Query Frame = 0


Query: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60
           MGKT  FDLE+ FAFYGAYHSNP+NIFIHVLFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120
           DHGLVLNFGF FTL+YAA YV+FDKRAGSMAALLCFVCWVGAS+I+N+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIEADIKEWKEKKEKLS 199
           AKI+ADI+EWKEKKEKLS
Sbjct: 181 AKIKADIREWKEKKEKLS 198

[Close]

BLAST of Carg18196 vs. TAIR 10
Match: AT1G18720.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 249.2 bits (635), Expect = 2.7e-66
Identity = 126/197 (63.96%), Postives = 151/197 (76.65%), Query Frame = 0


Query: 7   FDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLY-FTPSFYTIPKSPCGFDHGLV 66
           FDLE+HFAFYGAYHSNP+NI IH++FVWPIFF+ L+ L+  TP F     S  GF   L 
Sbjct: 8   FDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPIF---DPSQLGFSQSLT 67

Query: 67  L------NFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVL 126
           L      N GF+F LIYA  Y+  DK++G +AAL+CF CWVG+SF++ RLG S   KV L
Sbjct: 68  LDGVLRFNVGFIFALIYALFYIGLDKKSGFVAALMCFSCWVGSSFLAVRLGSSLALKVGL 127

Query: 127 AAQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASV 186
           A+QL CWT QF+GHGVFEKRAPALLDNL QAFLMAPFFV LEVLQ++F YEPYPGF A V
Sbjct: 128 ASQLLCWTGQFVGHGVFEKRAPALLDNLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQARV 187

Query: 187 QAKIEADIKEWKEKKEK 197
            AK+E+DIKE++ KK+K
Sbjct: 188 NAKVESDIKEYRAKKQK 201

[Close]

BLAST of Carg18196 vs. TAIR 10
Match: AT1G74440.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 246.1 bits (627), Expect = 2.3e-65
Identity = 121/200 (60.50%), Postives = 149/200 (74.50%), Query Frame = 0


Query: 3   KTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCG--- 62
           +    DLE+HFAFYGAYHSNP+NI IH LFVWP  F TL++LY TP    +  S  G   
Sbjct: 4   RIGLLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPI--LDHSQLGFLK 63

Query: 63  ---FDHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWK 122
              FD  L L+ GF  T+ YA  Y+  DK++G +AALLCF CW+G+SF++ RLG+S T K
Sbjct: 64  SLTFDGVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHSLTLK 123

Query: 123 VVLAAQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFS 182
           V +A+QL CWT QF+GHG+FEKRAPALLDNL QAFLM PFFV LEVLQ++F YEPYPGF 
Sbjct: 124 VGVASQLLCWTGQFLGHGLFEKRAPALLDNLLQAFLMGPFFVLLEVLQSVFGYEPYPGFK 183

Query: 183 ASVQAKIEADIKEWKEKKEK 197
           A V +KIE+ IKEW+EKK++
Sbjct: 184 ARVDSKIESGIKEWREKKKQ 201

The following BLAST results are available for this feature:

BLAST of Carg18196 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022933403.1	1.1e-109	100.00	uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita mosch...	[more]
XP_023531593.1	2.3e-107	98.46	uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo ...	[more]
XP_022972978.1	1.1e-106	96.97	uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita maxim...	[more]
XP_038880038.1	5.3e-104	93.43	2-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida]	[more]
XP_004138174.1	8.5e-102	91.92	uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus]...	[more]

BLAST of Carg18196 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O13737	3.2e-19	37.57	2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 97...	[more]
P25338	1.3e-15	33.15	2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATC...	[more]

BLAST of Carg18196 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F4N0	5.3e-110	100.00	uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita mos...	[more]
A0A6J1IBP8	5.5e-107	96.97	uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita max...	[more]
A0A0A0LP54	4.1e-102	91.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1	[more]
A0A5A7UQ13	5.4e-102	91.41	Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BV50	5.4e-102	91.41	uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo O...	[more]

BLAST of Carg18196 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18720.1	2.7e-66	63.96	Protein of unknown function (DUF962)	[more]
AT1G74440.1	2.3e-65	60.50	Protein of unknown function (DUF962)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 180..198
None	No IPR available	PANTHER	PTHR28026:SF8	YGL010W-LIKE PROTEIN	coord: 1..197
IPR009305	2-hydroxy-palmitic acid dioxygenase Mpo1-like	PFAM	PF06127	DUF962	coord: 7..165 e-value: 2.2E-30 score: 104.6
IPR009305	2-hydroxy-palmitic acid dioxygenase Mpo1-like	PANTHER	PTHR28026	DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)	coord: 1..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18196-RA	Carg18196-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046521	sphingoid catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane