Carg18175 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18175
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionIsochorismate synthase
LocationCarg_Chr11: 778347 .. 782251 (-)
RNA-Seq ExpressionCarg18175
SyntenyCarg18175
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATAAAGAAAAGGGTAAGAATGAGTATTCCCAGTAAGGCTATTTCTTCCACTTCTCAAACTTCACCCACCGGACCCTGTGTTGGGTTCGCCCAGCATCTGAGTCCGGGCCGCCGCATACCAACACTCGTAATTTGGCCAGGGCGGTGGCGGAGGAGATGGGGTGGGGTGGTTTGTCAGTCGCCGGCGATGGATGGGTGCAGAGGAGACCACGGAGGCGCCGTCGAGACGATGGAGACTCGAACTTTCCCGGCGGTTCCGACGGTGGGCTTGGCGACGGAGAATCTGAGGGGGTTGGTGGAAAAGTTGAAATCTGAGAAACCGGCGTTTAGTTCAGGAATTATTCGGCTCCAAGTAAGCCTCCTTCCAACTCTCCCGGACCTCTTTCAAATGCCTCGCATTACGCATTTCTTATCTCTTCCATGTACGTGGAATGAGAACCGCCACGTGCGGTAATTTATTATTATTATTATTTTTTTTTTTTAAAGGGTGGCTGTAACATTTCTTTTCTCTCCTCTGTTTGGCATCTCGGATTATACTAAACCGGAATAGACCTTTTCCTCCAAAATAAATAAATAAATAAATAAATAAATAAATACCCATTTTCTTAATATATGATTTTTTTTAATAATAATTATCAACTAAACCTAATAATTAGTAAAAAGAAAAGAAAATCTAACAAAAAATAAATAAAGTAATAAAATTGAGTTGAAATTCTGTTGACTTTGTGCACATTCTGAGACTGAAGGAGGGTTGACTATTTTGCAGCTTCCAATCCAACGGCACATTAAGGCGATTGATTGGCTTTGTTCTCAGCCTCTGCTTCTTCCTCGTATCTTCTTCTCCGGCAGGAGCAGAGCCATTGATTCTGATCCTCTCCCCGCTGTCAGCTCCAATGCCCACCACCGCCTCGCCAGCGTCGCAGGTGTGGGCTCTGCAGTCGTGTTCTGCCGCCATCATCAGTTTTCGAACTCTGATTGGGCGTCTATAAAGAGGTGGTAAACTCATTTTGCCTCTGTTTATATTCACTCCGCTATCTGTTCGACGAAATGCCTAGGCTAATATTCTGTAAATTATAGGTTCCTGTCAAAGAAATGCCCCCTGATTCGTGCTTATGGAGGTATTCGCTTTGATTCACGTGCGAACATATCGCCTGAATGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTACAGACTTCTTTCTCCAATCTCGTTTATCGCAATTGGATAATGGACGCCAAAATCACAGGTTGAGTTTGATGAGTTCGAGGATGATTCGATTCTTGCCGCTACTGTAGCTTGGGATCATGCTTGTTCTTGGACTTGGGAAAATGCTGTTGAATCGCTTCAATCCACAATCGAACAGGTCGACTATTTCATACAATTTGATTCTCTTTCTTCATTGCATTGAAAGGAAGAATATTCTCCCATAGATAATCACTCCCTAGTAAATTTCTTATCATTCAGGTTTCTTCTGATTATGTAAATGTACAAAGAGATCTTCCTCAGATATCCATACTTAGCCACAATCACGTTCCCACCAAAACATTCTGGGATCATGCAATTAACAGGGCGCTGCAAGTGATTGACCAATGCGACTCAGATCTTACCAAGGCACTCACCAATTCTTCACTCCTACCACAAATTTATTTATGTTTCTATTCATCAGTAAAATCGGCTGTCTGATCTTATGCATTATATTTAGGTCGTACTTGCACGCAGTACCCGAATAGTGACGAGCGTAGATGTCGATCCTATTACATGGGTTGCATGTTTACAGGTAGCTATTGCATGGGTTGCATGTTTATGCAGTAATTTTGTTTCCTGAGTCTAGTTTTGATCGTATCAGAAAGAGGGGAAAAACTCTTATCAGTTTTGTGTCCAGCCATCCAATGCTCCTGCCTTCATAGGAAATACGGTATGTAACAAAATTTCATACGCCTCATTGTATCAGTCAACCATTTCATTTTGTTGCCTAATTTCAAAATGGGGAATATCTGTGTTCCGGCTTAAGATGGTACTCATTAAAGATGTTTTCAATTAGTTTCCTACCCGTTTGGGTTTTGTAATATTTATTTTCTTACTAACTTTGGCTTTGATATGTTCGTAATAGCCTGAGCGGCTATTCCATAGAGAAGGACTTAGTATTATATGTGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAGATCGGCTATTGAAGACCTTAAAATAGAACAAGATTTATTATCCAAGTTGGTTTCATCTCTAAATTCTTATTTTGTCTACTCAATGATGCTGTAACCCTGTTGCCTCAACCAATGAGCTGCGGGCGGGAGTTTTCACTAACTCTACTTTCTATGTGCAGCCCAAAGGATGACCTTGAGTTTTCGATTGTGAGGGAAGCCATTAAAAGGAAGTTAGAGGCAAGTTTGAGCTTCAGTCTTGCTCTTTCGCTATCTTGCTTTCTGTCGATGAAATGAAACTTCCCCTTAAACAGCCTCCCCTAAATCGAGACTCGACTTCTTCTCTGGAGCCCTCGAGCAAAGCACACCCTTTATTTGACCCTTGTAACAAGGGCATGACTCTGATATTATGTTAGGAACCACATACCTTCACAATAGTATGATATTGTCTACTTTGAGCATAAGCTCTCGTGACTTTACTTTTGGTTTCCCCAAAAGGCCTCATACCAATGGAGATGGTATTCCTTACTTATAAATTCATGATATTCTCCTTAGTTAGCCAACGTGGGACTGCTCTCCCAATAATCTTCAACAATGGTTGTCCCTGATAACGACCTGTCCCACTAAGTTTGGTCTGTGGCTGCTATGAGCTATGAAAAATGATATTTGCAGTGCTTCCAAACCGTGAATTTGTTCTATTTTTTGTCATCGTTTTTCAAGCACCAAACAACATTTACATTGGTTTCTCAATAAGCTGCTGAAATTTATAGGCAATTACGAAGAGGACAACATTCTGAGAATATTGCAGATTCTTAGCCAGCTTAATCCTGTTTGTAGGCTGTATGTGATGCTGTAGTCGTTGAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAACTAGCAGGCAGATTGAGAACCAAGGATGATGAGGTAAGAATCTCCAAATATTTATGTCCCTGTAAACTAAACTTCCCTCACGTGAATATACCGCTCTAACTCTACTTTGTTGCTTGAAAAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTTCCAACAGAGGAGGCTCGAGATTTAATTGCAGAAACTGGTAAGGTTCATGAGTGCGTGATCCTTTCTATTAGCTCCCCAAGCCAATCCAAGTTATGGATGGTTTCCTTCAAAATGCTGATTATGGGTCTTGTAAGTCTGTCGTCTTCATCCTTCTGTATACATTTAAGAACTTGGGATATTTATGCAGAGTTATTTGATAGAGGGATGTACGCCGGGCCTGTTGGTTGGTTTGGAGGAGAAGAGAGTGAGTTTGCTGTGGGCATTAGATCAGCATTAGTGGAGACAGTAAGATGGCGTCTACCATATGAGCTACAAACTTAAATACTTCCATTGAATCCTTACTCGAATTTAAAATTTCTAGAGCTTAGACATCATTTCCATTTACAATTCTCTGAGTTCTTACGGTCTTTGATTATAGGGTCTTGGTGCCTTGGTCTATGCGGGAACAGGGATAGTAAAAGGAAGCAATCCGTCTTTGGAGTGGGATGAATTGGAGCTCAAGATATCTCAGGTACTCTGGAACTTACATCTTTTGTCTCGAAAATGCTCTACTTAGTAGCTCAATTTTCATACCTTATACCTTTGATTTGCATATTTGCAGGTTACCAGGTCGCTTAAACTGGAATTGGCTTGGAGCACCCACGTTTGA

mRNA sequence

GAAATAAAGAAAAGGGTAAGAATGAGTATTCCCAGTAAGGCTATTTCTTCCACTTCTCAAACTTCACCCACCGGACCCTGTGTTGGGTTCGCCCAGCATCTGAGTCCGGGCCGCCGCATACCAACACTCGTAATTTGGCCAGGGCGGTGGCGGAGGAGATGGGGTGGGGTGGTTTGTCAGTCGCCGGCGATGGATGGGTGCAGAGGAGACCACGGAGGCGCCGTCGAGACGATGGAGACTCGAACTTTCCCGGCGGTTCCGACGGTGGGCTTGGCGACGGAGAATCTGAGGGGGTTGGTGGAAAAGTTGAAATCTGAGAAACCGGCGTTTAGTTCAGGAATTATTCGGCTCCAACTTCCAATCCAACGGCACATTAAGGCGATTGATTGGCTTTGTTCTCAGCCTCTGCTTCTTCCTCGTATCTTCTTCTCCGGCAGGAGCAGAGCCATTGATTCTGATCCTCTCCCCGCTGTCAGCTCCAATGCCCACCACCGCCTCGCCAGCGTCGCAGGTGTGGGCTCTGCAGTCGTGTTCTGCCGCCATCATCAGTTTTCGAACTCTGATTGGGCGTCTATAAAGAGGTTCCTGTCAAAGAAATGCCCCCTGATTCGTGCTTATGGAGGTATTCGCTTTGATTCACGTGCGAACATATCGCCTGAATGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAGTTCGAGGATGATTCGATTCTTGCCGCTACTGTAGCTTGGGATCATGCTTGTTCTTGGACTTGGGAAAATGCTGTTGAATCGCTTCAATCCACAATCGAACAGGTTTCTTCTGATTATGTAAATGTACAAAGAGATCTTCCTCAGATATCCATACTTAGCCACAATCACGTTCCCACCAAAACATTCTGGGATCATGCAATTAACAGGGCGCTGCAAGTCGTACTTGCACGCAGTACCCGAATAGTGACGAGCGTAGATGTCGATCCTATTACATGGGTTGCATGTTTACAGAAAGAGGGGAAAAACTCTTATCAGTTTTGTGTCCAGCCATCCAATGCTCCTGCCTTCATAGGAAATACGCCTGAGCGGCTATTCCATAGAGAAGGACTTAGTATTATATGTGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAGATCGGCTATTGAAGACCTTAAAATAGAACAAGATTTATTATCCAAGTTGGCTGTATGTGATGCTGTAGTCGTTGAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAACTAGCAGGCAGATTGAGAACCAAGGATGATGAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTTCCAACAGAGGAGGCTCGAGATTTAATTGCAGAAACTGGTAAGGTTCATGAGTGCGTGATCCTTTCTATTAGCTCCCCAAGCCAATCCAAGTTATGGATGGTTTCCTTCAAAATGCTGATTATGGGTCTTGTAAGTCTGTCGTCTTCATCCTTCTGTATACATTTAAGAACTTGGGATATTTATGCAGAGTTATTTGATAGAGGGATGTACGCCGGGCCTGTTGGTTGGTTTGGAGGAGAAGAGAGTGAGTTTGCTGTGGGCATTAGATCAGCATTAGTGGAGACAGGTCTTGGTGCCTTGGTCTATGCGGGAACAGGGATAGTAAAAGGAAGCAATCCGTCTTTGGAGTGGGATGAATTGGAGCTCAAGATATCTCAGGTTACCAGGTCGCTTAAACTGGAATTGGCTTGGAGCACCCACGTTTGA

Coding sequence (CDS)

ATGAGTATTCCCAGTAAGGCTATTTCTTCCACTTCTCAAACTTCACCCACCGGACCCTGTGTTGGGTTCGCCCAGCATCTGAGTCCGGGCCGCCGCATACCAACACTCGTAATTTGGCCAGGGCGGTGGCGGAGGAGATGGGGTGGGGTGGTTTGTCAGTCGCCGGCGATGGATGGGTGCAGAGGAGACCACGGAGGCGCCGTCGAGACGATGGAGACTCGAACTTTCCCGGCGGTTCCGACGGTGGGCTTGGCGACGGAGAATCTGAGGGGGTTGGTGGAAAAGTTGAAATCTGAGAAACCGGCGTTTAGTTCAGGAATTATTCGGCTCCAACTTCCAATCCAACGGCACATTAAGGCGATTGATTGGCTTTGTTCTCAGCCTCTGCTTCTTCCTCGTATCTTCTTCTCCGGCAGGAGCAGAGCCATTGATTCTGATCCTCTCCCCGCTGTCAGCTCCAATGCCCACCACCGCCTCGCCAGCGTCGCAGGTGTGGGCTCTGCAGTCGTGTTCTGCCGCCATCATCAGTTTTCGAACTCTGATTGGGCGTCTATAAAGAGGTTCCTGTCAAAGAAATGCCCCCTGATTCGTGCTTATGGAGGTATTCGCTTTGATTCACGTGCGAACATATCGCCTGAATGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAGTTCGAGGATGATTCGATTCTTGCCGCTACTGTAGCTTGGGATCATGCTTGTTCTTGGACTTGGGAAAATGCTGTTGAATCGCTTCAATCCACAATCGAACAGGTTTCTTCTGATTATGTAAATGTACAAAGAGATCTTCCTCAGATATCCATACTTAGCCACAATCACGTTCCCACCAAAACATTCTGGGATCATGCAATTAACAGGGCGCTGCAAGTCGTACTTGCACGCAGTACCCGAATAGTGACGAGCGTAGATGTCGATCCTATTACATGGGTTGCATGTTTACAGAAAGAGGGGAAAAACTCTTATCAGTTTTGTGTCCAGCCATCCAATGCTCCTGCCTTCATAGGAAATACGCCTGAGCGGCTATTCCATAGAGAAGGACTTAGTATTATATGTGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAGATCGGCTATTGAAGACCTTAAAATAGAACAAGATTTATTATCCAAGTTGGCTGTATGTGATGCTGTAGTCGTTGAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAACTAGCAGGCAGATTGAGAACCAAGGATGATGAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTTCCAACAGAGGAGGCTCGAGATTTAATTGCAGAAACTGGTAAGGTTCATGAGTGCGTGATCCTTTCTATTAGCTCCCCAAGCCAATCCAAGTTATGGATGGTTTCCTTCAAAATGCTGATTATGGGTCTTGTAAGTCTGTCGTCTTCATCCTTCTGTATACATTTAAGAACTTGGGATATTTATGCAGAGTTATTTGATAGAGGGATGTACGCCGGGCCTGTTGGTTGGTTTGGAGGAGAAGAGAGTGAGTTTGCTGTGGGCATTAGATCAGCATTAGTGGAGACAGGTCTTGGTGCCTTGGTCTATGCGGGAACAGGGATAGTAAAAGGAAGCAATCCGTCTTTGGAGTGGGATGAATTGGAGCTCAAGATATCTCAGGTTACCAGGTCGCTTAAACTGGAATTGGCTTGGAGCACCCACGTTTGA

Protein sequence

MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINRALQVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELAWSTHV
Homology
BLAST of Carg18175 vs. NCBI nr
Match: KAG7021502.1 (Isochorismate synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300

Query: 301 ALQVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREG 360
           ALQVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREG
Sbjct: 301 ALQVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREG 360

Query: 361 LSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKLPRIQHLYAQLA 420
           LSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKLPRIQHLYAQLA
Sbjct: 361 LSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKLPRIQHLYAQLA 420

Query: 421 GRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVS 480
           GRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVS
Sbjct: 421 GRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVS 480

Query: 481 FKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVET 540
           FKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVET
Sbjct: 481 FKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVET 540

Query: 541 GLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELAWSTHV 587
           GLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELAWSTHV
Sbjct: 541 GLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELAWSTHV 586

BLAST of Carg18175 vs. NCBI nr
Match: XP_023530897.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.2e-291
Identity = 520/615 (84.55%), Postives = 526/615 (85.53%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVI PGRWRRRWG VVCQSPAMDGC
Sbjct: 4   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVISPGRWRRRWGRVVCQSPAMDGC 63

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPI RHIKA
Sbjct: 64  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIHRHIKA 123

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           IDWLCSQPLLLPRIFFSGRSRAIDSD LP+VSSNAHHR+ASVAGVGSAVVFCRHHQFSNS
Sbjct: 124 IDWLCSQPLLLPRIFFSGRSRAIDSDSLPSVSSNAHHRVASVAGVGSAVVFCRHHQFSNS 183

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKRFLSKKCPLIRAYGGIRFDSR NISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 184 DWASIKRFLSKKCPLIRAYGGIRFDSRPNISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 243

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQST+EQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 244 TVAWDHACSWTWENAVESLQSTVEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDSAINR 303

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSVDVDP+TWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 304 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPVTWVACLQKEGKNSYQFCVQPSNAPAFIG 363

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARG+SAIEDL+IEQDLLS                 KL
Sbjct: 364 NTPERLFHREGLSIICEALAATRARGKSAIEDLRIEQDLLSNPKDDLEFSIVREAIKRKL 423

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRT DDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 424 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTNDDEFEILSTLHPTPAVCGFPTEEAR 483

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 484 DLIAET--------------------------------------------------ELFD 543

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 587
           RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 544 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 568

BLAST of Carg18175 vs. NCBI nr
Match: XP_022932292.1 (isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.9e-291
Identity = 523/610 (85.74%), Postives = 525/610 (86.07%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISS   TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS                 KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
           RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 557

BLAST of Carg18175 vs. NCBI nr
Match: XP_022972451.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 1001.5 bits (2588), Expect = 3.0e-288
Identity = 514/610 (84.26%), Postives = 524/610 (85.90%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISSTSQTSPTGPCVGFAQHLSPGRRIPT VIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQ+HIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           I WLCSQPLLLPRIFFSGRSRAIDSDPLP+VSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKRFLSKKCPLIRA+GGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDRAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSV+VDP+TWVACLQKEG++SYQFCVQP+NAPAFIG
Sbjct: 301 ALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPANAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARG+SAIEDLKIEQDLLS                 KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
           RGMYAGPVGWFGGEESEFAVGIRSALVETG GALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVKGSNPSLEWDELELKISQ 560

BLAST of Carg18175 vs. NCBI nr
Match: XP_022932298.1 (isochorismate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 935.6 bits (2417), Expect = 2.0e-268
Identity = 483/571 (84.59%), Postives = 485/571 (84.94%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISS   TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS                 KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 518

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGL 543
           RGMYAGPVGWFGGEESEFAVGIRSALVET L
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETSL 518

BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match: Q9M9V6 (Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 PE=2 SV=2)

HSP 1 Score: 539.7 bits (1389), Expect = 4.2e-152
Identity = 294/558 (52.69%), Postives = 363/558 (65.05%), Query Frame = 0

Query: 56  AMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQ 115
           +M+GC  DH   + T+ETRT   VP+   ATE L   V  LKS+ P FSSGI+RLQ+PI+
Sbjct: 50  SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109

Query: 116 RHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS---NAHHRLASVAGVGSAVVFC 175
           + I AIDWL +Q  +LPR FFS RS +   D L   SS   ++ H   SVAG+GSAV F 
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFR 169

Query: 176 RHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEF 235
               FS+ DW SI+RFLS K PLIRAYGG+RFD    I+ EWE FGSFYF VPQVEFDEF
Sbjct: 170 DLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEF 229

Query: 236 EDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKT 295
              S+LAATVAWD+  SWT ENA+E+LQ T+ QVSS  + ++R+   + ++S NHVP++ 
Sbjct: 230 GGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEG 289

Query: 296 FWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQP 355
            +  A+N AL+           VVLARS+RI+T  D+DPI W+A LQ EG+++YQFC+QP
Sbjct: 290 AYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQP 349

Query: 356 SNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSK---------- 415
             APAFIGNTPERLFHR+ L +  EALAATR RG S + +++IE+DLL+           
Sbjct: 350 PGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIV 409

Query: 416 --------LAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVC 475
                     +CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVC
Sbjct: 410 RENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVC 469

Query: 476 GFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTW 535
           G P EEAR LI +                                               
Sbjct: 470 GCPVEEARLLIKQ----------------------------------------------- 529

Query: 536 DIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWD 582
               E FDRGMYAGP+G+FGG ESEF+VGIRSALVE GLGAL+YAGTGIV GSNPS EW+
Sbjct: 530 ---IESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIVSGSNPSSEWN 557

BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match: Q9S7H8 (Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 PE=1 SV=2)

HSP 1 Score: 519.6 bits (1337), Expect = 4.5e-146
Identity = 288/570 (50.53%), Postives = 361/570 (63.33%), Query Frame = 0

Query: 54  SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
           S +M+GC GD    + T+ETRT  AV +   ATE L   V +LKS+ P+FSSG++RLQ+P
Sbjct: 45  SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104

Query: 114 IQRHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-----------NAHHRLASV 173
           I + I AIDWL +Q  + PR FFS RS     D L  +++           ++   L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164

Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYF 233
           AG+GSAV F     FS+ DW SI+RFLS   PLIRAYGG+RFD    I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224

Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISI 293
            VPQVEF+EF   S+LAAT+AWD   SWT ENA+E+LQ T+ QVSS  + ++     +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284

Query: 294 LSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEG 353
           LS NHVPTK  +  A+ +AL+           VVLAR++RI+T  D+DPI W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344

Query: 354 KNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS- 413
            ++YQFC+QP  APAFIGNTPERLF R  L +  EALAATR R  S+  D++IE+DLL+ 
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404

Query: 414 ----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
                           KL  +CD VVV+P K+VRKL R+QHLY+QLAGRL  +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464

Query: 474 STLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSS 533
           + LHPTPAVCG P EEAR LI E                                     
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKE------------------------------------- 524

Query: 534 SSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIV 584
                         E FDRGMYAGP+G+FGGEESEFAVGIRSALVE GLGAL+YAGTGIV
Sbjct: 525 -------------IESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGALIYAGTGIV 564

BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match: Q9ZPC0 (Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.5e-145
Identity = 282/574 (49.13%), Postives = 367/574 (63.94%), Query Frame = 0

Query: 54  SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
           S +M+GC GD    V T+ETRT PAV T  LA E L   V  LKS  P+  SGIIRL++P
Sbjct: 52  SLSMNGCNGDPRAPVGTIETRTLPAVSTPALAMERLSSAVANLKSTLPSAQSGIIRLEVP 111

Query: 114 IQRHIKAIDWLCSQPL--LLPRIFFSGRSRAIDSD----------------PLPAVSSNA 173
           I+ HI+A+DWL SQ    LLPR +FSGRS+   SD                 L  +S+++
Sbjct: 112 IEEHIEALDWLHSQDQKNLLPRCYFSGRSQVTFSDFTSNDLTNRNGSAANGHLQRISTSS 171

Query: 174 HHR-LASVAGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEW 233
             + L SVAGVGSAV+F   + FS  DW SIKRFLSK CPLIRAYG IRFD+R +I+PEW
Sbjct: 172 DDKNLVSVAGVGSAVLFRSPNPFSFDDWLSIKRFLSKNCPLIRAYGAIRFDARPHIAPEW 231

Query: 234 EPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQ 293
           + FGSFYF+VPQVEFDE    S++AATVAWD+A S T++ A+ +LQ+T+EQVSS    ++
Sbjct: 232 KAFGSFYFVVPQVEFDELHGSSMIAATVAWDNALSLTYQQAIVALQTTMEQVSSTVSKLR 291

Query: 294 RDLPQISILSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITW 353
           +D+   S++S  ++P +T WD  +NR L+           VVLAR ++++T+ D+DP+ W
Sbjct: 292 QDVSHTSLVSKANIPDRTSWDLTLNRVLEEIGNKYSPLTKVVLARRSQVITTSDIDPLAW 351

Query: 354 VACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLK 413
           ++  + +GK++YQFC+QP  APAFIGNTPE+LF R+ L++  EALAATRARG S   DL+
Sbjct: 352 LSSFKADGKDAYQFCLQPHEAPAFIGNTPEQLFGRDQLTVFSEALAATRARGESDSLDLQ 411

Query: 414 IEQDLLS-----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRT 473
           +  DL S                 KL A+C +V  EP KSVRKL RIQHLYA+ AGRLR+
Sbjct: 412 MAHDLFSSPKDNHEFAIVRENIRQKLDAICTSVETEPMKSVRKLKRIQHLYARFAGRLRS 471

Query: 474 KDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLI 533
           +DDEF+ILS+LHPTPAVCGFP E+AR  IAE                             
Sbjct: 472 EDDEFKILSSLHPTPAVCGFPMEDARKFIAEN---------------------------- 531

Query: 534 MGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGAL 580
                                 E+FDRG+YAGPVG+FGG +S+F+VGIRSAL+    GAL
Sbjct: 532 ----------------------EMFDRGLYAGPVGFFGGAQSDFSVGIRSALIGKDAGAL 575

BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match: Q15KI9 (Protein PHYLLO, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PHYLLO PE=2 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 1.8e-25
Identity = 77/228 (33.77%), Postives = 115/228 (50.44%), Query Frame = 0

Query: 49  GVVCQSPAMD-GCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGI 108
           GV    P MD     D    V+   TRT P   T+ L  E+L+  +++LK+  P  SSG+
Sbjct: 56  GVQFDGPIMDRDVNLDDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKSSSGV 115

Query: 109 IRLQLPIQRHIKAIDWLCSQPL---LLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAG 168
           +R Q+ +    KA+ W CSQP    + P  F S           P+  S        V G
Sbjct: 116 LRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLS------KDTVEPSYKSLYVKEPHGVFG 175

Query: 169 VGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYG--GIRFDSRANISPEWEPFGSFYF 228
           +G+A  F       ++  + IK FLS +  ++ AYG   I F+  + ++ +    GS YF
Sbjct: 176 IGNAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK---DGSSYF 235

Query: 229 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDY 271
            VPQ+E DE E+ SILA T+AW+ + S+T E  + S + +I QVSS +
Sbjct: 236 FVPQIELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSHF 274

BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match: P23973 (Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF PE=3 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 1.7e-23
Identity = 101/411 (24.57%), Postives = 170/411 (41.36%), Query Frame = 0

Query: 199 YGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVES 258
           +GG  FD       +W+ F    F VP +         +L  T          W +  E 
Sbjct: 119 FGGFSFDPCEERGSQWDHFSEGDFFVPAL---------MLTMTAEGPFLTVNRWVSGGED 178

Query: 259 LQSTIEQV---SSDYVNVQRDLPQISILSHNHVPTKTFWDHAINRAL---------QVVL 318
            ++ +E +   +++++         ++++      K  W  AI  A          +VVL
Sbjct: 179 AEAVLEGLKAFAAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVL 238

Query: 319 ARSTRIVTS--VDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREGLSII 378
           AR   +     + ++P+  +  L  + + SY F ++      F+G +PERL  R+G +++
Sbjct: 239 ARELLLTFDGPIQIEPV--LKTLLDDQQTSYVFAIE-QEGKTFVGASPERLIKRDGGTVM 298

Query: 379 CEALAATRARGRSAIEDLKIEQDLLS---KLAVCDAVVVEPHKS---------------V 438
              LA +  RG +  +D +I  +LL+    L   D VV   H +               +
Sbjct: 299 SSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDGPVL 358

Query: 439 RKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVIL 498
            K   +QHL+  + G+LR     F+++  LHPTPA+ G P E+A D+I E          
Sbjct: 359 YKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIRE---------- 418

Query: 499 SISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEE 558
                                                    E   RG YA P+GW   ++
Sbjct: 419 ----------------------------------------IEPMSRGWYAAPIGWIDSQD 466

Query: 559 S-EFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSL 577
           + EFAV IRS L+E G  A ++AG GIV+ S P  E++E ++K+  +  +L
Sbjct: 479 NGEFAVAIRSGLIE-GSTARLFAGCGIVEDSEPISEYEETQIKLKPMISAL 466

BLAST of Carg18175 vs. ExPASy TrEMBL
Match: A0A6J1EW89 (Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.4e-291
Identity = 523/610 (85.74%), Postives = 525/610 (86.07%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISS   TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS                 KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
           RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 557

BLAST of Carg18175 vs. ExPASy TrEMBL
Match: A0A6J1I605 (Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.5e-288
Identity = 514/610 (84.26%), Postives = 524/610 (85.90%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISSTSQTSPTGPCVGFAQHLSPGRRIPT VIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQ+HIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           I WLCSQPLLLPRIFFSGRSRAIDSDPLP+VSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKRFLSKKCPLIRA+GGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDRAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSV+VDP+TWVACLQKEG++SYQFCVQP+NAPAFIG
Sbjct: 301 ALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPANAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARG+SAIEDLKIEQDLLS                 KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
           RGMYAGPVGWFGGEESEFAVGIRSALVETG GALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVKGSNPSLEWDELELKISQ 560

BLAST of Carg18175 vs. ExPASy TrEMBL
Match: A0A6J1F1A3 (Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 9.8e-269
Identity = 483/571 (84.59%), Postives = 485/571 (84.94%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISS   TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS                 KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 518

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGL 543
           RGMYAGPVGWFGGEESEFAVGIRSALVET L
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETSL 518

BLAST of Carg18175 vs. ExPASy TrEMBL
Match: A0A6J1EW06 (isochorismate synthase 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=4 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 7.0e-267
Identity = 482/592 (81.42%), Postives = 484/592 (81.76%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISS   TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKL 420
           NT                                         AVCDAVVVEPHKSVRKL
Sbjct: 361 NT-----------------------------------------AVCDAVVVEPHKSVRKL 420

Query: 421 PRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSIS 480
           PRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAET            
Sbjct: 421 PRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAET------------ 480

Query: 481 SPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEF 540
                                                 ELFDRGMYAGPVGWFGGEESEF
Sbjct: 481 --------------------------------------ELFDRGMYAGPVGWFGGEESEF 498

Query: 541 AVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELA 582
           AVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELA
Sbjct: 541 AVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELA 498

BLAST of Carg18175 vs. ExPASy TrEMBL
Match: A0A6J1I8P4 (Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.6e-266
Identity = 475/571 (83.19%), Postives = 485/571 (84.94%), Query Frame = 0

Query: 1   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
           MSIP+KAISSTSQTSPTGPCVGFAQHLSPGRRIPT VIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1   MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
           R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQ+HIKA
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120

Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
           I WLCSQPLLLPRIFFSGRSRAIDSDPLP+VSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180

Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
           DWASIKRFLSKKCPLIRA+GGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240

Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
           TVAWDHACSWTWENAVESLQSTIEQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDRAINR 300

Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
           ALQ           VVLARSTRIVTSV+VDP+TWVACLQKEG++SYQFCVQP+NAPAFIG
Sbjct: 301 ALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPANAPAFIG 360

Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
           NTPERLFHREGLSIICEALAATRARG+SAIEDLKIEQDLLS                 KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420

Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
            AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480

Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
           DLIAET                                                  ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 521

Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGL 543
           RGMYAGPVGWFGGEESEFAVGIRSALVET L
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETSL 521

BLAST of Carg18175 vs. TAIR 10
Match: AT1G18870.1 (isochorismate synthase 2 )

HSP 1 Score: 539.7 bits (1389), Expect = 3.0e-153
Identity = 294/558 (52.69%), Postives = 363/558 (65.05%), Query Frame = 0

Query: 56  AMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQ 115
           +M+GC  DH   + T+ETRT   VP+   ATE L   V  LKS+ P FSSGI+RLQ+PI+
Sbjct: 50  SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109

Query: 116 RHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS---NAHHRLASVAGVGSAVVFC 175
           + I AIDWL +Q  +LPR FFS RS +   D L   SS   ++ H   SVAG+GSAV F 
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFR 169

Query: 176 RHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEF 235
               FS+ DW SI+RFLS K PLIRAYGG+RFD    I+ EWE FGSFYF VPQVEFDEF
Sbjct: 170 DLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEF 229

Query: 236 EDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKT 295
              S+LAATVAWD+  SWT ENA+E+LQ T+ QVSS  + ++R+   + ++S NHVP++ 
Sbjct: 230 GGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEG 289

Query: 296 FWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQP 355
            +  A+N AL+           VVLARS+RI+T  D+DPI W+A LQ EG+++YQFC+QP
Sbjct: 290 AYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQP 349

Query: 356 SNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSK---------- 415
             APAFIGNTPERLFHR+ L +  EALAATR RG S + +++IE+DLL+           
Sbjct: 350 PGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIV 409

Query: 416 --------LAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVC 475
                     +CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVC
Sbjct: 410 RENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVC 469

Query: 476 GFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTW 535
           G P EEAR LI +                                               
Sbjct: 470 GCPVEEARLLIKQ----------------------------------------------- 529

Query: 536 DIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWD 582
               E FDRGMYAGP+G+FGG ESEF+VGIRSALVE GLGAL+YAGTGIV GSNPS EW+
Sbjct: 530 ---IESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIVSGSNPSSEWN 557

BLAST of Carg18175 vs. TAIR 10
Match: AT1G18870.2 (isochorismate synthase 2 )

HSP 1 Score: 538.9 bits (1387), Expect = 5.1e-153
Identity = 294/557 (52.78%), Postives = 362/557 (64.99%), Query Frame = 0

Query: 57  MDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQR 116
           M+GC  DH   + T+ETRT   VP+   ATE L   V  LKS+ P FSSGI+RLQ+PI++
Sbjct: 1   MNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIEQ 60

Query: 117 HIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS---NAHHRLASVAGVGSAVVFCR 176
            I AIDWL +Q  +LPR FFS RS +   D L   SS   ++ H   SVAG+GSAV F  
Sbjct: 61  KIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFRD 120

Query: 177 HHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 236
              FS+ DW SI+RFLS K PLIRAYGG+RFD    I+ EWE FGSFYF VPQVEFDEF 
Sbjct: 121 LDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEFG 180

Query: 237 DDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTF 296
             S+LAATVAWD+  SWT ENA+E+LQ T+ QVSS  + ++R+   + ++S NHVP++  
Sbjct: 181 GSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEGA 240

Query: 297 WDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPS 356
           +  A+N AL+           VVLARS+RI+T  D+DPI W+A LQ EG+++YQFC+QP 
Sbjct: 241 YYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQPP 300

Query: 357 NAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSK----------- 416
            APAFIGNTPERLFHR+ L +  EALAATR RG S + +++IE+DLL+            
Sbjct: 301 GAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIVR 360

Query: 417 -------LAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 476
                    +CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVCG
Sbjct: 361 ENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVCG 420

Query: 477 FPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWD 536
            P EEAR LI +                                                
Sbjct: 421 CPVEEARLLIKQ------------------------------------------------ 480

Query: 537 IYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDE 582
              E FDRGMYAGP+G+FGG ESEF+VGIRSALVE GLGAL+YAGTGIV GSNPS EW+E
Sbjct: 481 --IESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIVSGSNPSSEWNE 507

BLAST of Carg18175 vs. TAIR 10
Match: AT1G74710.1 (ADC synthase superfamily protein )

HSP 1 Score: 519.6 bits (1337), Expect = 3.2e-147
Identity = 288/570 (50.53%), Postives = 361/570 (63.33%), Query Frame = 0

Query: 54  SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
           S +M+GC GD    + T+ETRT  AV +   ATE L   V +LKS+ P+FSSG++RLQ+P
Sbjct: 45  SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104

Query: 114 IQRHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-----------NAHHRLASV 173
           I + I AIDWL +Q  + PR FFS RS     D L  +++           ++   L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164

Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYF 233
           AG+GSAV F     FS+ DW SI+RFLS   PLIRAYGG+RFD    I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224

Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISI 293
            VPQVEF+EF   S+LAAT+AWD   SWT ENA+E+LQ T+ QVSS  + ++     +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284

Query: 294 LSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEG 353
           LS NHVPTK  +  A+ +AL+           VVLAR++RI+T  D+DPI W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344

Query: 354 KNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS- 413
            ++YQFC+QP  APAFIGNTPERLF R  L +  EALAATR R  S+  D++IE+DLL+ 
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404

Query: 414 ----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
                           KL  +CD VVV+P K+VRKL R+QHLY+QLAGRL  +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464

Query: 474 STLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSS 533
           + LHPTPAVCG P EEAR LI E                                     
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKE------------------------------------- 524

Query: 534 SSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIV 584
                         E FDRGMYAGP+G+FGGEESEFAVGIRSALVE GLGAL+YAGTGIV
Sbjct: 525 -------------IESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGALIYAGTGIV 564

BLAST of Carg18175 vs. TAIR 10
Match: AT1G74710.2 (ADC synthase superfamily protein )

HSP 1 Score: 514.6 bits (1324), Expect = 1.0e-145
Identity = 285/559 (50.98%), Postives = 355/559 (63.51%), Query Frame = 0

Query: 54  SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
           S +M+GC GD    + T+ETRT  AV +   ATE L   V +LKS+ P+FSSG++RLQ+P
Sbjct: 45  SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104

Query: 114 IQRHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-----------NAHHRLASV 173
           I + I AIDWL +Q  + PR FFS RS     D L  +++           ++   L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164

Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYF 233
           AG+GSAV F     FS+ DW SI+RFLS   PLIRAYGG+RFD    I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224

Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISI 293
            VPQVEF+EF   S+LAAT+AWD   SWT ENA+E+LQ T+ QVSS  + ++     +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284

Query: 294 LSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEG 353
           LS NHVPTK  +  A+ +AL+           VVLAR++RI+T  D+DPI W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344

Query: 354 KNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS- 413
            ++YQFC+QP  APAFIGNTPERLF R  L +  EALAATR R  S+  D++IE+DLL+ 
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404

Query: 414 ----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
                           KL  +CD VVV+P K+VRKL R+QHLY+QLAGRL  +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464

Query: 474 STLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSS 533
           + LHPTPAVCG P EEAR LI E                                     
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKE------------------------------------- 524

Query: 534 SSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIV 573
                         E FDRGMYAGP+G+FGGEESEFAVGIRSALVE GLGAL+YAGTGIV
Sbjct: 525 -------------IESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGALIYAGTGIV 553

BLAST of Carg18175 vs. TAIR 10
Match: AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )

HSP 1 Score: 119.0 bits (297), Expect = 1.3e-26
Identity = 77/228 (33.77%), Postives = 115/228 (50.44%), Query Frame = 0

Query: 49  GVVCQSPAMD-GCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGI 108
           GV    P MD     D    V+   TRT P   T+ L  E+L+  +++LK+  P  SSG+
Sbjct: 56  GVQFDGPIMDRDVNLDDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKSSSGV 115

Query: 109 IRLQLPIQRHIKAIDWLCSQPL---LLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAG 168
           +R Q+ +    KA+ W CSQP    + P  F S           P+  S        V G
Sbjct: 116 LRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLS------KDTVEPSYKSLYVKEPHGVFG 175

Query: 169 VGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYG--GIRFDSRANISPEWEPFGSFYF 228
           +G+A  F       ++  + IK FLS +  ++ AYG   I F+  + ++ +    GS YF
Sbjct: 176 IGNAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK---DGSSYF 235

Query: 229 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDY 271
            VPQ+E DE E+ SILA T+AW+ + S+T E  + S + +I QVSS +
Sbjct: 236 FVPQIELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSHF 274

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021502.10.0e+00100.00Isochorismate synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_023530897.12.2e-29184.55isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022932292.12.9e-29185.74isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata][more]
XP_022972451.13.0e-28884.26isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima][more]
XP_022932298.12.0e-26884.59isochorismate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M9V64.2e-15252.69Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 ... [more]
Q9S7H84.5e-14650.53Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 ... [more]
Q9ZPC06.5e-14549.13Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1[more]
Q15KI91.8e-2533.77Protein PHYLLO, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PHYLLO PE=2 SV=... [more]
P239731.7e-2324.57Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF ... [more]
Match NameE-valueIdentityDescription
A0A6J1EW891.4e-29185.74Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1[more]
A0A6J1I6051.5e-28884.26Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1[more]
A0A6J1F1A39.8e-26984.59Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1[more]
A0A6J1EW067.0e-26781.42isochorismate synthase 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX... [more]
A0A6J1I8P41.6e-26683.19Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G18870.13.0e-15352.69isochorismate synthase 2 [more]
AT1G18870.25.1e-15352.78isochorismate synthase 2 [more]
AT1G74710.13.2e-14750.53ADC synthase superfamily protein [more]
AT1G74710.21.0e-14550.98ADC synthase superfamily protein [more]
AT1G68890.11.3e-2633.77magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-su... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 256..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR47253:SF4ISOCHORISMATE SYNTHASE 2, CHLOROPLASTICcoord: 507..582
NoneNo IPR availablePANTHERPTHR47253:SF4ISOCHORISMATE SYNTHASE 2, CHLOROPLASTICcoord: 53..458
IPR015890Chorismate-utilising enzyme, C-terminalPFAMPF00425Chorismate_bindcoord: 302..390
e-value: 4.6E-11
score: 42.8
coord: 389..458
e-value: 2.5E-12
score: 46.9
coord: 508..568
e-value: 2.1E-14
score: 53.7
IPR005801ADC synthaseGENE3D3.60.120.10Anthranilate synthasecoord: 85..578
e-value: 1.8E-79
score: 269.9
IPR005801ADC synthaseSUPERFAMILY56322ADC synthasecoord: 156..457
IPR005801ADC synthaseSUPERFAMILY56322ADC synthasecoord: 508..578
IPR044250Isochorismate synthase MenF-likePANTHERPTHR47253FAMILY NOT NAMEDcoord: 53..458
IPR044250Isochorismate synthase MenF-likePANTHERPTHR47253FAMILY NOT NAMEDcoord: 507..582

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18175-RACarg18175-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042372 phylloquinone biosynthetic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0009536 plastid
molecular_function GO:0008909 isochorismate synthase activity