Homology
BLAST of Carg18175 vs. NCBI nr
Match:
KAG7021502.1 (Isochorismate synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
Query: 301 ALQVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREG 360
ALQVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREG
Sbjct: 301 ALQVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREG 360
Query: 361 LSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKLPRIQHLYAQLA 420
LSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKLPRIQHLYAQLA
Sbjct: 361 LSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKLPRIQHLYAQLA 420
Query: 421 GRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVS 480
GRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVS
Sbjct: 421 GRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVS 480
Query: 481 FKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVET 540
FKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVET
Sbjct: 481 FKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVET 540
Query: 541 GLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELAWSTHV 587
GLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELAWSTHV
Sbjct: 541 GLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELAWSTHV 586
BLAST of Carg18175 vs. NCBI nr
Match:
XP_023530897.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1011.9 bits (2615), Expect = 2.2e-291
Identity = 520/615 (84.55%), Postives = 526/615 (85.53%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVI PGRWRRRWG VVCQSPAMDGC
Sbjct: 4 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVISPGRWRRRWGRVVCQSPAMDGC 63
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPI RHIKA
Sbjct: 64 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIHRHIKA 123
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
IDWLCSQPLLLPRIFFSGRSRAIDSD LP+VSSNAHHR+ASVAGVGSAVVFCRHHQFSNS
Sbjct: 124 IDWLCSQPLLLPRIFFSGRSRAIDSDSLPSVSSNAHHRVASVAGVGSAVVFCRHHQFSNS 183
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKRFLSKKCPLIRAYGGIRFDSR NISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 184 DWASIKRFLSKKCPLIRAYGGIRFDSRPNISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 243
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQST+EQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 244 TVAWDHACSWTWENAVESLQSTVEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDSAINR 303
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSVDVDP+TWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 304 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPVTWVACLQKEGKNSYQFCVQPSNAPAFIG 363
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARG+SAIEDL+IEQDLLS KL
Sbjct: 364 NTPERLFHREGLSIICEALAATRARGKSAIEDLRIEQDLLSNPKDDLEFSIVREAIKRKL 423
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRT DDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 424 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTNDDEFEILSTLHPTPAVCGFPTEEAR 483
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 484 DLIAET--------------------------------------------------ELFD 543
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 587
RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 544 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 568
BLAST of Carg18175 vs. NCBI nr
Match:
XP_022932292.1 (isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 1011.5 bits (2614), Expect = 2.9e-291
Identity = 523/610 (85.74%), Postives = 525/610 (86.07%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISS TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 557
BLAST of Carg18175 vs. NCBI nr
Match:
XP_022972451.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima])
HSP 1 Score: 1001.5 bits (2588), Expect = 3.0e-288
Identity = 514/610 (84.26%), Postives = 524/610 (85.90%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISSTSQTSPTGPCVGFAQHLSPGRRIPT VIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQ+HIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
I WLCSQPLLLPRIFFSGRSRAIDSDPLP+VSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKRFLSKKCPLIRA+GGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDRAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSV+VDP+TWVACLQKEG++SYQFCVQP+NAPAFIG
Sbjct: 301 ALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPANAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARG+SAIEDLKIEQDLLS KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
RGMYAGPVGWFGGEESEFAVGIRSALVETG GALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVKGSNPSLEWDELELKISQ 560
BLAST of Carg18175 vs. NCBI nr
Match:
XP_022932298.1 (isochorismate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata])
HSP 1 Score: 935.6 bits (2417), Expect = 2.0e-268
Identity = 483/571 (84.59%), Postives = 485/571 (84.94%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISS TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 518
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGL 543
RGMYAGPVGWFGGEESEFAVGIRSALVET L
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETSL 518
BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match:
Q9M9V6 (Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 PE=2 SV=2)
HSP 1 Score: 539.7 bits (1389), Expect = 4.2e-152
Identity = 294/558 (52.69%), Postives = 363/558 (65.05%), Query Frame = 0
Query: 56 AMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQ 115
+M+GC DH + T+ETRT VP+ ATE L V LKS+ P FSSGI+RLQ+PI+
Sbjct: 50 SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109
Query: 116 RHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS---NAHHRLASVAGVGSAVVFC 175
+ I AIDWL +Q +LPR FFS RS + D L SS ++ H SVAG+GSAV F
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFR 169
Query: 176 RHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEF 235
FS+ DW SI+RFLS K PLIRAYGG+RFD I+ EWE FGSFYF VPQVEFDEF
Sbjct: 170 DLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEF 229
Query: 236 EDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKT 295
S+LAATVAWD+ SWT ENA+E+LQ T+ QVSS + ++R+ + ++S NHVP++
Sbjct: 230 GGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEG 289
Query: 296 FWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQP 355
+ A+N AL+ VVLARS+RI+T D+DPI W+A LQ EG+++YQFC+QP
Sbjct: 290 AYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQP 349
Query: 356 SNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSK---------- 415
APAFIGNTPERLFHR+ L + EALAATR RG S + +++IE+DLL+
Sbjct: 350 PGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIV 409
Query: 416 --------LAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVC 475
+CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVC
Sbjct: 410 RENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVC 469
Query: 476 GFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTW 535
G P EEAR LI +
Sbjct: 470 GCPVEEARLLIKQ----------------------------------------------- 529
Query: 536 DIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWD 582
E FDRGMYAGP+G+FGG ESEF+VGIRSALVE GLGAL+YAGTGIV GSNPS EW+
Sbjct: 530 ---IESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIVSGSNPSSEWN 557
BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match:
Q9S7H8 (Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 PE=1 SV=2)
HSP 1 Score: 519.6 bits (1337), Expect = 4.5e-146
Identity = 288/570 (50.53%), Postives = 361/570 (63.33%), Query Frame = 0
Query: 54 SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
S +M+GC GD + T+ETRT AV + ATE L V +LKS+ P+FSSG++RLQ+P
Sbjct: 45 SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104
Query: 114 IQRHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-----------NAHHRLASV 173
I + I AIDWL +Q + PR FFS RS D L +++ ++ L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164
Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYF 233
AG+GSAV F FS+ DW SI+RFLS PLIRAYGG+RFD I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224
Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISI 293
VPQVEF+EF S+LAAT+AWD SWT ENA+E+LQ T+ QVSS + ++ +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284
Query: 294 LSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEG 353
LS NHVPTK + A+ +AL+ VVLAR++RI+T D+DPI W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344
Query: 354 KNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS- 413
++YQFC+QP APAFIGNTPERLF R L + EALAATR R S+ D++IE+DLL+
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404
Query: 414 ----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
KL +CD VVV+P K+VRKL R+QHLY+QLAGRL +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464
Query: 474 STLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSS 533
+ LHPTPAVCG P EEAR LI E
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKE------------------------------------- 524
Query: 534 SSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIV 584
E FDRGMYAGP+G+FGGEESEFAVGIRSALVE GLGAL+YAGTGIV
Sbjct: 525 -------------IESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGALIYAGTGIV 564
BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match:
Q9ZPC0 (Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1)
HSP 1 Score: 515.8 bits (1327), Expect = 6.5e-145
Identity = 282/574 (49.13%), Postives = 367/574 (63.94%), Query Frame = 0
Query: 54 SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
S +M+GC GD V T+ETRT PAV T LA E L V LKS P+ SGIIRL++P
Sbjct: 52 SLSMNGCNGDPRAPVGTIETRTLPAVSTPALAMERLSSAVANLKSTLPSAQSGIIRLEVP 111
Query: 114 IQRHIKAIDWLCSQPL--LLPRIFFSGRSRAIDSD----------------PLPAVSSNA 173
I+ HI+A+DWL SQ LLPR +FSGRS+ SD L +S+++
Sbjct: 112 IEEHIEALDWLHSQDQKNLLPRCYFSGRSQVTFSDFTSNDLTNRNGSAANGHLQRISTSS 171
Query: 174 HHR-LASVAGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEW 233
+ L SVAGVGSAV+F + FS DW SIKRFLSK CPLIRAYG IRFD+R +I+PEW
Sbjct: 172 DDKNLVSVAGVGSAVLFRSPNPFSFDDWLSIKRFLSKNCPLIRAYGAIRFDARPHIAPEW 231
Query: 234 EPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQ 293
+ FGSFYF+VPQVEFDE S++AATVAWD+A S T++ A+ +LQ+T+EQVSS ++
Sbjct: 232 KAFGSFYFVVPQVEFDELHGSSMIAATVAWDNALSLTYQQAIVALQTTMEQVSSTVSKLR 291
Query: 294 RDLPQISILSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITW 353
+D+ S++S ++P +T WD +NR L+ VVLAR ++++T+ D+DP+ W
Sbjct: 292 QDVSHTSLVSKANIPDRTSWDLTLNRVLEEIGNKYSPLTKVVLARRSQVITTSDIDPLAW 351
Query: 354 VACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLK 413
++ + +GK++YQFC+QP APAFIGNTPE+LF R+ L++ EALAATRARG S DL+
Sbjct: 352 LSSFKADGKDAYQFCLQPHEAPAFIGNTPEQLFGRDQLTVFSEALAATRARGESDSLDLQ 411
Query: 414 IEQDLLS-----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRT 473
+ DL S KL A+C +V EP KSVRKL RIQHLYA+ AGRLR+
Sbjct: 412 MAHDLFSSPKDNHEFAIVRENIRQKLDAICTSVETEPMKSVRKLKRIQHLYARFAGRLRS 471
Query: 474 KDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLI 533
+DDEF+ILS+LHPTPAVCGFP E+AR IAE
Sbjct: 472 EDDEFKILSSLHPTPAVCGFPMEDARKFIAEN---------------------------- 531
Query: 534 MGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGAL 580
E+FDRG+YAGPVG+FGG +S+F+VGIRSAL+ GAL
Sbjct: 532 ----------------------EMFDRGLYAGPVGFFGGAQSDFSVGIRSALIGKDAGAL 575
BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match:
Q15KI9 (Protein PHYLLO, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PHYLLO PE=2 SV=2)
HSP 1 Score: 119.0 bits (297), Expect = 1.8e-25
Identity = 77/228 (33.77%), Postives = 115/228 (50.44%), Query Frame = 0
Query: 49 GVVCQSPAMD-GCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGI 108
GV P MD D V+ TRT P T+ L E+L+ +++LK+ P SSG+
Sbjct: 56 GVQFDGPIMDRDVNLDDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKSSSGV 115
Query: 109 IRLQLPIQRHIKAIDWLCSQPL---LLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAG 168
+R Q+ + KA+ W CSQP + P F S P+ S V G
Sbjct: 116 LRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLS------KDTVEPSYKSLYVKEPHGVFG 175
Query: 169 VGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYG--GIRFDSRANISPEWEPFGSFYF 228
+G+A F ++ + IK FLS + ++ AYG I F+ + ++ + GS YF
Sbjct: 176 IGNAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK---DGSSYF 235
Query: 229 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDY 271
VPQ+E DE E+ SILA T+AW+ + S+T E + S + +I QVSS +
Sbjct: 236 FVPQIELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSHF 274
BLAST of Carg18175 vs. ExPASy Swiss-Prot
Match:
P23973 (Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF PE=3 SV=2)
HSP 1 Score: 112.5 bits (280), Expect = 1.7e-23
Identity = 101/411 (24.57%), Postives = 170/411 (41.36%), Query Frame = 0
Query: 199 YGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVES 258
+GG FD +W+ F F VP + +L T W + E
Sbjct: 119 FGGFSFDPCEERGSQWDHFSEGDFFVPAL---------MLTMTAEGPFLTVNRWVSGGED 178
Query: 259 LQSTIEQV---SSDYVNVQRDLPQISILSHNHVPTKTFWDHAINRAL---------QVVL 318
++ +E + +++++ ++++ K W AI A +VVL
Sbjct: 179 AEAVLEGLKAFAAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVL 238
Query: 319 ARSTRIVTS--VDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREGLSII 378
AR + + ++P+ + L + + SY F ++ F+G +PERL R+G +++
Sbjct: 239 ARELLLTFDGPIQIEPV--LKTLLDDQQTSYVFAIE-QEGKTFVGASPERLIKRDGGTVM 298
Query: 379 CEALAATRARGRSAIEDLKIEQDLLS---KLAVCDAVVVEPHKS---------------V 438
LA + RG + +D +I +LL+ L D VV H + +
Sbjct: 299 SSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDGPVL 358
Query: 439 RKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVIL 498
K +QHL+ + G+LR F+++ LHPTPA+ G P E+A D+I E
Sbjct: 359 YKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIRE---------- 418
Query: 499 SISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEE 558
E RG YA P+GW ++
Sbjct: 419 ----------------------------------------IEPMSRGWYAAPIGWIDSQD 466
Query: 559 S-EFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSL 577
+ EFAV IRS L+E G A ++AG GIV+ S P E++E ++K+ + +L
Sbjct: 479 NGEFAVAIRSGLIE-GSTARLFAGCGIVEDSEPISEYEETQIKLKPMISAL 466
BLAST of Carg18175 vs. ExPASy TrEMBL
Match:
A0A6J1EW89 (Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1)
HSP 1 Score: 1011.5 bits (2614), Expect = 1.4e-291
Identity = 523/610 (85.74%), Postives = 525/610 (86.07%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISS TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 557
BLAST of Carg18175 vs. ExPASy TrEMBL
Match:
A0A6J1I605 (Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1)
HSP 1 Score: 1001.5 bits (2588), Expect = 1.5e-288
Identity = 514/610 (84.26%), Postives = 524/610 (85.90%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISSTSQTSPTGPCVGFAQHLSPGRRIPT VIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQ+HIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
I WLCSQPLLLPRIFFSGRSRAIDSDPLP+VSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKRFLSKKCPLIRA+GGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDRAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSV+VDP+TWVACLQKEG++SYQFCVQP+NAPAFIG
Sbjct: 301 ALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPANAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARG+SAIEDLKIEQDLLS KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 540
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQ 582
RGMYAGPVGWFGGEESEFAVGIRSALVETG GALVYAGTGIVKGSNPSLEWDELELKISQ
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVKGSNPSLEWDELELKISQ 560
BLAST of Carg18175 vs. ExPASy TrEMBL
Match:
A0A6J1F1A3 (Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1)
HSP 1 Score: 935.6 bits (2417), Expect = 9.8e-269
Identity = 483/571 (84.59%), Postives = 485/571 (84.94%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISS TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 518
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGL 543
RGMYAGPVGWFGGEESEFAVGIRSALVET L
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETSL 518
BLAST of Carg18175 vs. ExPASy TrEMBL
Match:
A0A6J1EW06 (isochorismate synthase 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=4 SV=1)
HSP 1 Score: 929.5 bits (2401), Expect = 7.0e-267
Identity = 482/592 (81.42%), Postives = 484/592 (81.76%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISS TSPTGPCVGFAQHLSPGRRIPTLVI+PGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISS---TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQRHIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKR LSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG
Sbjct: 301 ALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSKLAVCDAVVVEPHKSVRKL 420
NT AVCDAVVVEPHKSVRKL
Sbjct: 361 NT-----------------------------------------AVCDAVVVEPHKSVRKL 420
Query: 421 PRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETGKVHECVILSIS 480
PRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAET
Sbjct: 421 PRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAET------------ 480
Query: 481 SPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEF 540
ELFDRGMYAGPVGWFGGEESEF
Sbjct: 481 --------------------------------------ELFDRGMYAGPVGWFGGEESEF 498
Query: 541 AVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELA 582
AVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELA
Sbjct: 541 AVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELA 498
BLAST of Carg18175 vs. ExPASy TrEMBL
Match:
A0A6J1I8P4 (Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1)
HSP 1 Score: 928.3 bits (2398), Expect = 1.6e-266
Identity = 475/571 (83.19%), Postives = 485/571 (84.94%), Query Frame = 0
Query: 1 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVIWPGRWRRRWGGVVCQSPAMDGC 60
MSIP+KAISSTSQTSPTGPCVGFAQHLSPGRRIPT VIWPGRWRRRWGGVVCQSPAMDGC
Sbjct: 1 MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQRHIKA 120
R DHGGAVETMETRTFPAVPTVGLATENLRGLV KLKSEKPAFSSGIIRLQLPIQ+HIKA
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120
Query: 121 IDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
I WLCSQPLLLPRIFFSGRSRAIDSDPLP+VSSNAHHRLASVAGVGSAVVFCRHHQFSNS
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAGVGSAVVFCRHHQFSNS 180
Query: 181 DWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
DWASIKRFLSKKCPLIRA+GGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA
Sbjct: 181 DWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAA 240
Query: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINR 300
TVAWDHACSWTWENAVESLQSTIEQVSSD VNVQRDLPQISILSHNHVPTKTFWD AINR
Sbjct: 241 TVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDRAINR 300
Query: 301 ALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIG 360
ALQ VVLARSTRIVTSV+VDP+TWVACLQKEG++SYQFCVQP+NAPAFIG
Sbjct: 301 ALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPANAPAFIG 360
Query: 361 NTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS-----------------KL 420
NTPERLFHREGLSIICEALAATRARG+SAIEDLKIEQDLLS KL
Sbjct: 361 NTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKL 420
Query: 421 -AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR
Sbjct: 421 EAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEAR 480
Query: 481 DLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWDIYAELFD 540
DLIAET ELFD
Sbjct: 481 DLIAET--------------------------------------------------ELFD 521
Query: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETGL 543
RGMYAGPVGWFGGEESEFAVGIRSALVET L
Sbjct: 541 RGMYAGPVGWFGGEESEFAVGIRSALVETSL 521
BLAST of Carg18175 vs. TAIR 10
Match:
AT1G18870.1 (isochorismate synthase 2 )
HSP 1 Score: 539.7 bits (1389), Expect = 3.0e-153
Identity = 294/558 (52.69%), Postives = 363/558 (65.05%), Query Frame = 0
Query: 56 AMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQ 115
+M+GC DH + T+ETRT VP+ ATE L V LKS+ P FSSGI+RLQ+PI+
Sbjct: 50 SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109
Query: 116 RHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS---NAHHRLASVAGVGSAVVFC 175
+ I AIDWL +Q +LPR FFS RS + D L SS ++ H SVAG+GSAV F
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFR 169
Query: 176 RHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEF 235
FS+ DW SI+RFLS K PLIRAYGG+RFD I+ EWE FGSFYF VPQVEFDEF
Sbjct: 170 DLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEF 229
Query: 236 EDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKT 295
S+LAATVAWD+ SWT ENA+E+LQ T+ QVSS + ++R+ + ++S NHVP++
Sbjct: 230 GGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEG 289
Query: 296 FWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQP 355
+ A+N AL+ VVLARS+RI+T D+DPI W+A LQ EG+++YQFC+QP
Sbjct: 290 AYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQP 349
Query: 356 SNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSK---------- 415
APAFIGNTPERLFHR+ L + EALAATR RG S + +++IE+DLL+
Sbjct: 350 PGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIV 409
Query: 416 --------LAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVC 475
+CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVC
Sbjct: 410 RENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVC 469
Query: 476 GFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTW 535
G P EEAR LI +
Sbjct: 470 GCPVEEARLLIKQ----------------------------------------------- 529
Query: 536 DIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWD 582
E FDRGMYAGP+G+FGG ESEF+VGIRSALVE GLGAL+YAGTGIV GSNPS EW+
Sbjct: 530 ---IESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIVSGSNPSSEWN 557
BLAST of Carg18175 vs. TAIR 10
Match:
AT1G18870.2 (isochorismate synthase 2 )
HSP 1 Score: 538.9 bits (1387), Expect = 5.1e-153
Identity = 294/557 (52.78%), Postives = 362/557 (64.99%), Query Frame = 0
Query: 57 MDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLPIQR 116
M+GC DH + T+ETRT VP+ ATE L V LKS+ P FSSGI+RLQ+PI++
Sbjct: 1 MNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIEQ 60
Query: 117 HIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS---NAHHRLASVAGVGSAVVFCR 176
I AIDWL +Q +LPR FFS RS + D L SS ++ H SVAG+GSAV F
Sbjct: 61 KIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFRD 120
Query: 177 HHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 236
FS+ DW SI+RFLS K PLIRAYGG+RFD I+ EWE FGSFYF VPQVEFDEF
Sbjct: 121 LDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEFG 180
Query: 237 DDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTF 296
S+LAATVAWD+ SWT ENA+E+LQ T+ QVSS + ++R+ + ++S NHVP++
Sbjct: 181 GSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEGA 240
Query: 297 WDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPS 356
+ A+N AL+ VVLARS+RI+T D+DPI W+A LQ EG+++YQFC+QP
Sbjct: 241 YYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQPP 300
Query: 357 NAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSK----------- 416
APAFIGNTPERLFHR+ L + EALAATR RG S + +++IE+DLL+
Sbjct: 301 GAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIVR 360
Query: 417 -------LAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 476
+CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVCG
Sbjct: 361 ENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVCG 420
Query: 477 FPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSSSSFCIHLRTWD 536
P EEAR LI +
Sbjct: 421 CPVEEARLLIKQ------------------------------------------------ 480
Query: 537 IYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEWDE 582
E FDRGMYAGP+G+FGG ESEF+VGIRSALVE GLGAL+YAGTGIV GSNPS EW+E
Sbjct: 481 --IESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIVSGSNPSSEWNE 507
BLAST of Carg18175 vs. TAIR 10
Match:
AT1G74710.1 (ADC synthase superfamily protein )
HSP 1 Score: 519.6 bits (1337), Expect = 3.2e-147
Identity = 288/570 (50.53%), Postives = 361/570 (63.33%), Query Frame = 0
Query: 54 SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
S +M+GC GD + T+ETRT AV + ATE L V +LKS+ P+FSSG++RLQ+P
Sbjct: 45 SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104
Query: 114 IQRHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-----------NAHHRLASV 173
I + I AIDWL +Q + PR FFS RS D L +++ ++ L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164
Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYF 233
AG+GSAV F FS+ DW SI+RFLS PLIRAYGG+RFD I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224
Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISI 293
VPQVEF+EF S+LAAT+AWD SWT ENA+E+LQ T+ QVSS + ++ +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284
Query: 294 LSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEG 353
LS NHVPTK + A+ +AL+ VVLAR++RI+T D+DPI W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344
Query: 354 KNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS- 413
++YQFC+QP APAFIGNTPERLF R L + EALAATR R S+ D++IE+DLL+
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404
Query: 414 ----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
KL +CD VVV+P K+VRKL R+QHLY+QLAGRL +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464
Query: 474 STLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSS 533
+ LHPTPAVCG P EEAR LI E
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKE------------------------------------- 524
Query: 534 SSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIV 584
E FDRGMYAGP+G+FGGEESEFAVGIRSALVE GLGAL+YAGTGIV
Sbjct: 525 -------------IESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGALIYAGTGIV 564
BLAST of Carg18175 vs. TAIR 10
Match:
AT1G74710.2 (ADC synthase superfamily protein )
HSP 1 Score: 514.6 bits (1324), Expect = 1.0e-145
Identity = 285/559 (50.98%), Postives = 355/559 (63.51%), Query Frame = 0
Query: 54 SPAMDGCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGIIRLQLP 113
S +M+GC GD + T+ETRT AV + ATE L V +LKS+ P+FSSG++RLQ+P
Sbjct: 45 SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104
Query: 114 IQRHIKAIDWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-----------NAHHRLASV 173
I + I AIDWL +Q + PR FFS RS D L +++ ++ L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164
Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYF 233
AG+GSAV F FS+ DW SI+RFLS PLIRAYGG+RFD I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224
Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISI 293
VPQVEF+EF S+LAAT+AWD SWT ENA+E+LQ T+ QVSS + ++ +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284
Query: 294 LSHNHVPTKTFWDHAINRALQ-----------VVLARSTRIVTSVDVDPITWVACLQKEG 353
LS NHVPTK + A+ +AL+ VVLAR++RI+T D+DPI W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344
Query: 354 KNSYQFCVQPSNAPAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLS- 413
++YQFC+QP APAFIGNTPERLF R L + EALAATR R S+ D++IE+DLL+
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404
Query: 414 ----------------KL-AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
KL +CD VVV+P K+VRKL R+QHLY+QLAGRL +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464
Query: 474 STLHPTPAVCGFPTEEARDLIAETGKVHECVILSISSPSQSKLWMVSFKMLIMGLVSLSS 533
+ LHPTPAVCG P EEAR LI E
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKE------------------------------------- 524
Query: 534 SSFCIHLRTWDIYAELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIV 573
E FDRGMYAGP+G+FGGEESEFAVGIRSALVE GLGAL+YAGTGIV
Sbjct: 525 -------------IESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGALIYAGTGIV 553
BLAST of Carg18175 vs. TAIR 10
Match:
AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )
HSP 1 Score: 119.0 bits (297), Expect = 1.3e-26
Identity = 77/228 (33.77%), Postives = 115/228 (50.44%), Query Frame = 0
Query: 49 GVVCQSPAMD-GCRGDHGGAVETMETRTFPAVPTVGLATENLRGLVEKLKSEKPAFSSGI 108
GV P MD D V+ TRT P T+ L E+L+ +++LK+ P SSG+
Sbjct: 56 GVQFDGPIMDRDVNLDDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKSSSGV 115
Query: 109 IRLQLPIQRHIKAIDWLCSQPL---LLPRIFFSGRSRAIDSDPLPAVSSNAHHRLASVAG 168
+R Q+ + KA+ W CSQP + P F S P+ S V G
Sbjct: 116 LRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLS------KDTVEPSYKSLYVKEPHGVFG 175
Query: 169 VGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAYG--GIRFDSRANISPEWEPFGSFYF 228
+G+A F ++ + IK FLS + ++ AYG I F+ + ++ + GS YF
Sbjct: 176 IGNAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK---DGSSYF 235
Query: 229 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDY 271
VPQ+E DE E+ SILA T+AW+ + S+T E + S + +I QVSS +
Sbjct: 236 FVPQIELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSHF 274
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7021502.1 | 0.0e+00 | 100.00 | Isochorismate synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrospe... | [more] |
XP_023530897.1 | 2.2e-291 | 84.55 | isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
XP_022932292.1 | 2.9e-291 | 85.74 | isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata] | [more] |
XP_022972451.1 | 3.0e-288 | 84.26 | isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima] | [more] |
XP_022932298.1 | 2.0e-268 | 84.59 | isochorismate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q9M9V6 | 4.2e-152 | 52.69 | Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 ... | [more] |
Q9S7H8 | 4.5e-146 | 50.53 | Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 ... | [more] |
Q9ZPC0 | 6.5e-145 | 49.13 | Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1 | [more] |
Q15KI9 | 1.8e-25 | 33.77 | Protein PHYLLO, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PHYLLO PE=2 SV=... | [more] |
P23973 | 1.7e-23 | 24.57 | Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1EW89 | 1.4e-291 | 85.74 | Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1 | [more] |
A0A6J1I605 | 1.5e-288 | 84.26 | Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1 | [more] |
A0A6J1F1A3 | 9.8e-269 | 84.59 | Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1 | [more] |
A0A6J1EW06 | 7.0e-267 | 81.42 | isochorismate synthase 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX... | [more] |
A0A6J1I8P4 | 1.6e-266 | 83.19 | Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1 | [more] |