Carg18134 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18134
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	CASP-like protein
Location	Carg_Chr11: 562693 .. 564996 (-)
RNA-Seq Expression	Carg18134
Synteny	Carg18134

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTGAGACGAAATTGAAAGCTGAAAGATCGTACGACTCTTCGTCCATGAAGAAGTCGTCGTCCAGAAATTCCGAGTCGTCAATTCACAGTCATATGGAGTCTCCTCACTCGCCGCTGCGTTTCCACTCGCCGCTCCGATCCGACGTCGGCGACAGTCTGTGACCAAGGTAGGGCCGGCTGCGAGTGGAGTCGAGGAGGATGGTGGTGGTGGTGGTGAAAGATCGCCGAAGCAAATACCGCCGCCGCCGAGGAGGTCGAAGGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTGTCTGAAGTGGCGGTTTGCTTGATTTCGTTTTCGGTTATGGCGTCTGATAAGACTAAGGGATGGAGCGGCGACTCCTTCGATCGTTACAAGGAATACAGGTTTCTTCTTCTTCTTACAATCTACTCGATGCCTAATGTCTATTACACTAGAGAATATCGTAAGTTGGATTTGCTTTTGGCTGTTCAGGAATTTGTACATTTTGATTTTGTTGATTTGTTTCAGAATTGACTACACTTGTCGTTTCCTCAATTCCTTGGGCTGTACAGTACTATTTTAGTTCATTGTTCAGTTAGCGTCAACTGGGACATGCATTGTCAATCTTTTACCATACCAATTAGCTTTCCATTATTTGAATCCTATAAATTTGCTTCCAAGGAGAGTCATGGCCTGAAACAATTATTGTGATTAATGATCTTGAAATTCTTCATTGTCATCACATGAGGTAGGTGCTTTAAATGTAGTCTTCAATTTCAAGGTACGTCTCATTGTAAATGACGAATGCCATGTTAAGAATGGAGGCGTCGCCTTCGTTCCAACCTATTGATTGCCAAGTTTTCAATGATTCACACTGTCCAAATGAATTCCTTTGCTAGACTATTTAATTCAGTGACCCACAACTTAAAGCATATTCTGAGTCTCTGACATGGCCACTTTTCTGTCTTTCCTCGTGTCATTTGCTGCTTTGAGTTGCAAGTTCTTAGTTCTCATGTTGGTTTTTGGTATAATTTCAAGAGTGTGTACTGTGAGTGAAGGATTATAGTGGAAGCAATCTCACTGTCAGGATTTTCGCATAATTTCCATGCCTTTTTGTGATCAATCATGTGTGACATTATTCTTAGCTTCCCTTGTATTGGAAGAATACATCAATTACCGGATATATCTCATATTTTGTCCAAATGATTTGAAGCTATCTCTTTTTGCAATTTTCCAGTAGTCTATATGATTTTGCCCGTCCTATGTTATCTGATCAAGCCCATTTGTTAACAGGTACTGCCTCACTGTTAATATAATTGCATTCGTGTATGCGGCATTTCAAGCGTTTGATCTTGTCTATACTCTAATCAAGAAGCAACATATGATACGTCATCGCGTCCGTTCTTACTTCGACTTCTTCATGGATCAGGTTAGTTGTGCAAATCTGCTGGTCTGAATGAATATTGTTTAATGTCTATAGCCATGTGTTGCACTGCTGCTCTGCTTCAATGAATGTGTTTCTATGGTTAAAGGTCTGTTTTGTACAGTTCTGTAGCCTGACGAATAGGATACTTAGGCTAAAAGAAACTCGCTGGATTTATTAGAGAATCCTTTTGGTGTCCCCACAAACTGACTAAGGTCTTTGTGAATTTGAAACGATGGTTCTGATCTCTAGTAAAGATGAGATCCCATATTAGTTGGAAAGAGGAATGAAGTCTTCCTTATAATAGTGTAGGACACCGCTCCCTACAAGATGCGTTTTAAAATCGTGAGGCTGACGGTGGTACGTAACGGATCAAAGCGGACAATATTTGCTAGCGGTAGGTTTGGATTGTTACAAATGGTATCAAAGACAAACACCGTGCAGTGTGCCAGTGAGGACGCTAGGCCCTCAAGGGGGTGGATTATGAGATCCCACATTGGTTGGAGAGGAAAACGGAGCATTCTTTATAAGGGTGTGGAAAACCTCTACTTAGAAGATACGTTTTAAAACCATGAGGCTAACGGCGATACGTAACAGACTAAAGCGGTTGATATCTGCTAGCGGTGGACTTGGACGGTTGTTCTACATCCACTAATGATGCAATCCTGGTTTTTTCAATTATATTGGCAGGTATTGGCATATCTTCTCATATCATCGTCTTCCTCCGCTGCGACACGTGTTGATGACTGGCAATCGAATTGGGGAAAAGATGAGTTCACACAGATGGCCAGTGCATCAATCAGCATGTCCTTCCTTGCCTTCGTTGCTTTTGCTGTTAGTTCGCTTATCTCTGGTTACAACCTTTGTAGCCGTGATCCTGCATGA

mRNA sequence

ATGATTGAGACGAAATTGAAAGCTGAAAGATCGTACGACTCTTCGTCCATGAAGAAGTCGTCGTCCAGAAATTCCGAGTCGTCAATTCACAGTCATATGGAGTCTCCTCACTCGCCGCTGCGTTTCCACTCGCCGCTCCGATCCGACGTCGGCGACAGGCCGGCTGCGAGTGGAGTCGAGGAGGATGGTGGTGGTGGTGGTGAAAGATCGCCGAAGCAAATACCGCCGCCGCCGAGGAGGTCGAAGGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTGTCTGAAGTGGCGGTTTGCTTGATTTCGTTTTCGGTTATGGCGTCTGATAAGACTAAGGGATGGAGCGGCGACTCCTTCGATCGTTACAAGGAATACAGGTACTGCCTCACTGTTAATATAATTGCATTCGTGTATGCGGCATTTCAAGCGTTTGATCTTGTCTATACTCTAATCAAGAAGCAACATATGATACGTCATCGCGTCCGTTCTTACTTCGACTTCTTCATGGATCAGATGGCCAGTGCATCAATCAGCATGTCCTTCCTTGCCTTCGTTGCTTTTGCTGTTAGTTCGCTTATCTCTGGTTACAACCTTTGTAGCCGTGATCCTGCATGA

Coding sequence (CDS)

ATGATTGAGACGAAATTGAAAGCTGAAAGATCGTACGACTCTTCGTCCATGAAGAAGTCGTCGTCCAGAAATTCCGAGTCGTCAATTCACAGTCATATGGAGTCTCCTCACTCGCCGCTGCGTTTCCACTCGCCGCTCCGATCCGACGTCGGCGACAGGCCGGCTGCGAGTGGAGTCGAGGAGGATGGTGGTGGTGGTGGTGAAAGATCGCCGAAGCAAATACCGCCGCCGCCGAGGAGGTCGAAGGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTGTCTGAAGTGGCGGTTTGCTTGATTTCGTTTTCGGTTATGGCGTCTGATAAGACTAAGGGATGGAGCGGCGACTCCTTCGATCGTTACAAGGAATACAGGTACTGCCTCACTGTTAATATAATTGCATTCGTGTATGCGGCATTTCAAGCGTTTGATCTTGTCTATACTCTAATCAAGAAGCAACATATGATACGTCATCGCGTCCGTTCTTACTTCGACTTCTTCATGGATCAGATGGCCAGTGCATCAATCAGCATGTCCTTCCTTGCCTTCGTTGCTTTTGCTGTTAGTTCGCTTATCTCTGGTTACAACCTTTGTAGCCGTGATCCTGCATGA

Protein sequence

MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQMASASISMSFLAFVAFAVSSLISGYNLCSRDPA

Homology

[Close]

BLAST of Carg18134 vs. NCBI nr
Match: KAG7021462.1 (CASP-like protein 4A3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 395.2 bits (1014), Expect = 3.5e-106
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVE 60
           MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVE
Sbjct: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVE 60

Query: 61  EDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDS 120
           EDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDS
Sbjct: 61  EDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDS 120

Query: 121 FDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQMASASIS 180
           FDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQMASASIS
Sbjct: 121 FDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQMASASIS 180

Query: 181 MSFLAFVAFAVSSLISGYNLCSRDPA 207
           MSFLAFVAFAVSSLISGYNLCSRDPA
Sbjct: 181 MSFLAFVAFAVSSLISGYNLCSRDPA 206

[Close]

BLAST of Carg18134 vs. NCBI nr
Match: XP_022925737.1 (CASP-like protein 4A2 [Cucurbita moschata])

HSP 1 Score: 340.5 bits (872), Expect = 1.0e-89
Identity = 205/319 (64.26%), Postives = 205/319 (64.26%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD-------- 60
           MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD        
Sbjct: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDPPETSPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPYASPEKLPDNSKSIVVVDVDNKSTQFSPLRSPNAGSRKLPEYANFPGDRLASSKVMFN 120

Query: 121 --------------RPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180
                          PAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV
Sbjct: 121 RAMKEEVPQSVTKVGPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180

Query: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 207
           AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM
Sbjct: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 240

[Close]

BLAST of Carg18134 vs. NCBI nr
Match: KAG6587474.1 (CASP-like protein 4A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 340.1 bits (871), Expect = 1.3e-89
Identity = 204/319 (63.95%), Postives = 205/319 (64.26%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD-------- 60
           MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD        
Sbjct: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDPPETSPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPYASPEKLPDNSKSIVVVDVDNKSTQFSPLRSPNAGSRKLPEYANFPGDRLASSKVMFN 120

Query: 121 --------------RPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180
                          PAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV
Sbjct: 121 RAMKEEVPQSVTKVGPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180

Query: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 207
           AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM
Sbjct: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 240

[Close]

BLAST of Carg18134 vs. NCBI nr
Match: XP_023530616.1 (CASP-like protein 4A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 199/319 (62.38%), Postives = 201/319 (63.01%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD-------- 60
           MIE KLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD        
Sbjct: 1   MIEMKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDPPETSPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPYASPEKLPDNFKSIVVVDVDNKSTQFSPLRSPNAGSRKLPEYANFPGDRSTSSKVMFN 120

Query: 121 --------------RPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180
                          PAA GVEEDGGGGG RSPKQ+PPPPRRSKDDALTKAALGFRV EV
Sbjct: 121 RAMKEEVPQSVTKVGPAAGGVEEDGGGGGGRSPKQMPPPPRRSKDDALTKAALGFRVCEV 180

Query: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 207
           AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM
Sbjct: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 240

[Close]

BLAST of Carg18134 vs. NCBI nr
Match: XP_022973205.1 (CASP-like protein 4A2 [Cucurbita maxima])

HSP 1 Score: 305.8 bits (782), Expect = 2.8e-79
Identity = 192/319 (60.19%), Postives = 193/319 (60.50%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD-------- 60
           MIETKLKAERSYD      SSSRNSESSI SHMESPHSPLRFHSPLRSDVGD        
Sbjct: 1   MIETKLKAERSYD------SSSRNSESSILSHMESPHSPLRFHSPLRSDVGDPPETSPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPYASPEKLPDNSKSIVVVDVDNKSTQFSPHRSPNAGSRKLPEYANFPGDRSASSKVMFN 120

Query: 121 --------------RPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180
                          PAASGVEEDGGGG  RSPKQIPPPPRRSKDDAL KAALGFRV EV
Sbjct: 121 RAMKEEVPQSVTKVGPAASGVEEDGGGG--RSPKQIPPPPRRSKDDALMKAALGFRVCEV 180

Query: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 207
           AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKK HM
Sbjct: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKHHM 240

[Close]

BLAST of Carg18134 vs. ExPASy Swiss-Prot
Match: D7LIR2 (CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_482607 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.5e-35
Identity = 98/229 (42.79%), Postives = 132/229 (57.64%), Query Frame = 0


Query: 12  YDSSSMKKSSSRNSESSIHSH----MESPHSPLRFHSPLRSDVGDRPAASGVEEDGG-GG 71
           + S S K+   R  ES  + +     E+P   +  H+  RS     P         G   
Sbjct: 46  HSSDSEKEDEKRRPESRRNKNPVKIEETPSPIVVVHNHNRSVKEVVPTRKTARVGSGRSS 105

Query: 72  GERSPKQIPPPPRRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKE 131
           G+RS   +    R  +++ +   ALGFR+SEV + LISFS+MA+DKTKGWSGDSFDRYKE
Sbjct: 106 GQRSGAVLAILRRSRREEIVKFVALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKE 165

Query: 132 YRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQ------------- 191
           YR+CL+VN++AF+YA+FQA DL Y L+K++H+I H +R  F+F +DQ             
Sbjct: 166 YRFCLSVNVVAFIYASFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMCASTAA 225

Query: 192 ------------------MASASISMSFLAFVAFAVSSLISGYNLCSRD 205
                             MASASI+MSFL F+AFA SSLISGYNL ++D
Sbjct: 226 VTRVDDWVSNWGKDDFTEMASASIAMSFLTFLAFAFSSLISGYNLFNQD 274

[Close]

BLAST of Carg18134 vs. ExPASy Swiss-Prot
Match: Q84WP5 (CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 9.6e-35
Identity = 96/224 (42.86%), Postives = 129/224 (57.59%), Query Frame = 0


Query: 8   AERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGG 67
           +E   + +  K  S RN          SP   +  H+    +V     ++ V   G   G
Sbjct: 55  SESEKEDAKSKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARV-GSGRSSG 114

Query: 68  ERSPKQIPPPPRRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKEY 127
           +RS        R  +++ +  +ALGFR+SEV + LISFS+MA+DKTKGWSGDSFDRYKEY
Sbjct: 115 QRSGAVSAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEY 174

Query: 128 RYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQ-------------- 187
           R+CL+VN++AFVY++FQA DL Y L+K++H+I H +R  F+F +DQ              
Sbjct: 175 RFCLSVNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAV 234

Query: 188 -----------------MASASISMSFLAFVAFAVSSLISGYNL 201
                            MASASI+MSFLAF+AFA SSLISGYNL
Sbjct: 235 TRVDDWVSNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

[Close]

BLAST of Carg18134 vs. ExPASy Swiss-Prot
Match: Q501G6 (CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 8.2e-34
Identity = 109/292 (37.33%), Postives = 138/292 (47.26%), Query Frame = 0


Query: 17  MKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGGERSPKQIP- 76
           MK+++S NSE+   S+ ESPHSPLRFHSPL SD GD P +  V  +G      +PK I  
Sbjct: 3   MKRTASSNSEA--QSYNESPHSPLRFHSPL-SDAGDLPESRYVSPEGSPFKIENPKSIVA 62

Query: 77  ---------------------PPPRRSK-------------------------------- 136
                                PPPRR +                                
Sbjct: 63  GNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDKSPSSMVVHNSWVREDGGQTT 122

Query: 137 -----------------------DDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGD 196
                                  DD ++  ALGFR++EV +C+ISFS+MA+DKT+GWSGD
Sbjct: 123 TRKAGAPINGEESTRTTVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQGWSGD 182

Query: 197 SFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMD------- 201
           S+DRYKEYRYCL VN+IAFVY+AF+A D    + K+ +MI       F F MD       
Sbjct: 183 SYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQLLAYLL 242

[Close]

BLAST of Carg18134 vs. ExPASy Swiss-Prot
Match: D7MMW4 (CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_919556 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.4e-33
Identity = 108/292 (36.99%), Postives = 138/292 (47.26%), Query Frame = 0


Query: 17  MKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGGERSPKQI-- 76
           MK++ S NSE+  +S+ ESPHSPLRFHSPL SD GD P +  V  +G      +PK I  
Sbjct: 3   MKRTVSSNSEA--YSYNESPHSPLRFHSPL-SDAGDLPESRYVSPEGSPFKIENPKSIVA 62

Query: 77  ---------------PPPP----------------------------------------- 136
                          PPPP                                         
Sbjct: 63  GNKLTQFSPLPSPIPPPPPQIPPPRRQRNARVPMNSSLDKSPSSMVVQNSWVREDGQQNT 122

Query: 137 -------------------RRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGD 196
                              R  +DD ++  ALGFR++EV +C+ISFS+MA+DKT+GWSGD
Sbjct: 123 TRKAGAPMNGEESATTAVNRARRDDLVSVTALGFRITEVILCVISFSIMAADKTQGWSGD 182

Query: 197 SFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMD------- 201
           S+DRYKEYRYCL VN+IAFVY+AF+A D    + K+ +M+       F F MD       
Sbjct: 183 SYDRYKEYRYCLAVNVIAFVYSAFEACDAACYMAKESYMMNCGFHDLFVFSMDQLLAYLL 242

[Close]

BLAST of Carg18134 vs. ExPASy Swiss-Prot
Match: Q9FNE8 (CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 78/208 (37.50%), Postives = 103/208 (49.52%), Query Frame = 0


Query: 31  SHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGGER----SPKQIPPPPRRSK-DDA 90
           SH  SPH+ +   S  +++   R   +G EE       R    S       PR SK    
Sbjct: 60  SHGSSPHTIIPTPSVAKTETPFR-VTNGEEEKKVSESRRQLRPSFSSSSSTPRESKWASL 119

Query: 91  LTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQA 150
           + KA LGFRV     CL+SFSVM SD+ KGW+ DSF  YKE+R+CL  N+I FVY+ F  
Sbjct: 120 IRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMI 179

Query: 151 FDLVYTLIKKQHMIRHRVRSYFDFFMDQM------------------------------- 203
            DLVY L       RH +R + +F +DQM                               
Sbjct: 180 CDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGADKFPDL 239

[Close]

BLAST of Carg18134 vs. ExPASy TrEMBL
Match: A0A6J1ECH3 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433061 PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.9e-90
Identity = 205/319 (64.26%), Postives = 205/319 (64.26%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD-------- 60
           MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD        
Sbjct: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDPPETSPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPYASPEKLPDNSKSIVVVDVDNKSTQFSPLRSPNAGSRKLPEYANFPGDRLASSKVMFN 120

Query: 121 --------------RPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180
                          PAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV
Sbjct: 121 RAMKEEVPQSVTKVGPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180

Query: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 207
           AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM
Sbjct: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 240

[Close]

BLAST of Carg18134 vs. ExPASy TrEMBL
Match: A0A6J1IAS4 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111471736 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.3e-79
Identity = 192/319 (60.19%), Postives = 193/319 (60.50%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD-------- 60
           MIETKLKAERSYD      SSSRNSESSI SHMESPHSPLRFHSPLRSDVGD        
Sbjct: 1   MIETKLKAERSYD------SSSRNSESSILSHMESPHSPLRFHSPLRSDVGDPPETSPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPYASPEKLPDNSKSIVVVDVDNKSTQFSPHRSPNAGSRKLPEYANFPGDRSASSKVMFN 120

Query: 121 --------------RPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180
                          PAASGVEEDGGGG  RSPKQIPPPPRRSKDDAL KAALGFRV EV
Sbjct: 121 RAMKEEVPQSVTKVGPAASGVEEDGGGG--RSPKQIPPPPRRSKDDALMKAALGFRVCEV 180

Query: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 207
           AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKK HM
Sbjct: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKHHM 240

[Close]

BLAST of Carg18134 vs. ExPASy TrEMBL
Match: A0A6J1C1D1 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111007480 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.2e-74
Identity = 176/321 (54.83%), Postives = 190/321 (59.19%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRP------ 60
           MIETKLK +RS DSSSM+KSSSRNSESS+HSHMESPHSPLRFHSPLRSD GD P      
Sbjct: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120

Query: 121 ------------------AASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVS 180
                             A  GVE  GGGGG RSPKQ+ P PRRSK DALTKAALGFRV 
Sbjct: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180

Query: 181 EVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQ 207
           EVAVCLISFSVMASDKT+GWSGDSFDRYKEYRYCLTVN+IAF+YAAFQAFDLVYTLIKK+
Sbjct: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240

[Close]

BLAST of Carg18134 vs. ExPASy TrEMBL
Match: A0A6J1E1W9 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430082 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 5.1e-71
Identity = 177/319 (55.49%), Postives = 185/319 (57.99%), Query Frame = 0


Query: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD-------- 60
           MIE KLK   S DSSSM+KSSSRNSE S+HS MESPHSPLRFHSPLRSDVGD        
Sbjct: 1   MIEAKLK---SIDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRSDVGDLPETPPYA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SPDTSPEKQPDNSKAIVLVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFN 120

Query: 121 --------------RPAASGVEEDGGGGGERSPKQIPPPPRRSKDDALTKAALGFRVSEV 180
                          PAA GVEE GGGGG RSPKQ+PP  RRSKDDALTKAALGFRV EV
Sbjct: 121 RAMKEDVPQSVTKVGPAAGGVEERGGGGG-RSPKQMPPMSRRSKDDALTKAALGFRVCEV 180

Query: 181 AVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHM 207
           AVCLISFSVMASDKT+GWSGDSFDRYKEYRYCLTVNIIAFVYA FQAFDLVYTL KK HM
Sbjct: 181 AVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHM 240

[Close]

BLAST of Carg18134 vs. ExPASy TrEMBL
Match: A0A0A0LNX3 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 9.6e-70
Identity = 178/333 (53.45%), Postives = 190/333 (57.06%), Query Frame = 0


Query: 1   MIETKLKAERSYD-SSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGD------- 60
           MIETKL+A+RS D SSSMKKSSSRNS+SS+HSHMESPHSPLRFHSPLRSD GD       
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAASRKPLENVNFVGDRSTSSKIMF 120

Query: 121 ---------------RPAASGVEED------------GGGGGERSP-KQIPPPPRRSKDD 180
                           P   G EE+            GGGGG RSP KQ+ P  RRSKDD
Sbjct: 121 NRAMKEEVPQSVTKVGPVGVGGEENGGGGGGGGGAGAGGGGGGRSPVKQMAPMSRRSKDD 180

Query: 181 ALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ 207
           AL KAALGFRV EVAVCLISFSVMASDKT+GWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ
Sbjct: 181 ALVKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ 240

[Close]

BLAST of Carg18134 vs. TAIR 10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 148.3 bits (373), Expect = 6.8e-36
Identity = 96/224 (42.86%), Postives = 129/224 (57.59%), Query Frame = 0


Query: 8   AERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGG 67
           +E   + +  K  S RN          SP   +  H+    +V     ++ V   G   G
Sbjct: 55  SESEKEDAKSKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARV-GSGRSSG 114

Query: 68  ERSPKQIPPPPRRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKEY 127
           +RS        R  +++ +  +ALGFR+SEV + LISFS+MA+DKTKGWSGDSFDRYKEY
Sbjct: 115 QRSGAVSAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEY 174

Query: 128 RYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQ-------------- 187
           R+CL+VN++AFVY++FQA DL Y L+K++H+I H +R  F+F +DQ              
Sbjct: 175 RFCLSVNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAV 234

Query: 188 -----------------MASASISMSFLAFVAFAVSSLISGYNL 201
                            MASASI+MSFLAF+AFA SSLISGYNL
Sbjct: 235 TRVDDWVSNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

[Close]

BLAST of Carg18134 vs. TAIR 10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 145.2 bits (365), Expect = 5.8e-35
Identity = 109/292 (37.33%), Postives = 138/292 (47.26%), Query Frame = 0


Query: 17  MKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGGERSPKQIP- 76
           MK+++S NSE+   S+ ESPHSPLRFHSPL SD GD P +  V  +G      +PK I  
Sbjct: 3   MKRTASSNSEA--QSYNESPHSPLRFHSPL-SDAGDLPESRYVSPEGSPFKIENPKSIVA 62

Query: 77  ---------------------PPPRRSK-------------------------------- 136
                                PPPRR +                                
Sbjct: 63  GNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDKSPSSMVVHNSWVREDGGQTT 122

Query: 137 -----------------------DDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGD 196
                                  DD ++  ALGFR++EV +C+ISFS+MA+DKT+GWSGD
Sbjct: 123 TRKAGAPINGEESTRTTVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQGWSGD 182

Query: 197 SFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMD------- 201
           S+DRYKEYRYCL VN+IAFVY+AF+A D    + K+ +MI       F F MD       
Sbjct: 183 SYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQLLAYLL 242

[Close]

BLAST of Carg18134 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 110.2 bits (274), Expect = 2.1e-24
Identity = 78/208 (37.50%), Postives = 103/208 (49.52%), Query Frame = 0


Query: 31  SHMESPHSPLRFHSPLRSDVGDRPAASGVEEDGGGGGER----SPKQIPPPPRRSK-DDA 90
           SH  SPH+ +   S  +++   R   +G EE       R    S       PR SK    
Sbjct: 60  SHGSSPHTIIPTPSVAKTETPFR-VTNGEEEKKVSESRRQLRPSFSSSSSTPRESKWASL 119

Query: 91  LTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQA 150
           + KA LGFRV     CL+SFSVM SD+ KGW+ DSF  YKE+R+CL  N+I FVY+ F  
Sbjct: 120 IRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMI 179

Query: 151 FDLVYTLIKKQHMIRHRVRSYFDFFMDQM------------------------------- 203
            DLVY L       RH +R + +F +DQM                               
Sbjct: 180 CDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGADKFPDL 239

[Close]

BLAST of Carg18134 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 53.5 bits (127), Expect = 2.3e-07
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 0


Query: 79  RRSKDDALTKAALGFRVSEVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAF 138
           R  ++D + KA+   R   +   LI+F +M S+K  G+ G +F+ Y+EYRY L ++II+ 
Sbjct: 37  RWKREDLIKKASPITRGICLLFSLIAFLIMVSNK-HGY-GRNFNDYEEYRYVLAISIIST 96

Query: 139 VYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQM------------------------ 198
           +Y A+Q F        K+ +   R     DF  DQ+                        
Sbjct: 97  LYTAWQTF----AHFSKREIFDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFREGQD 156

Query: 199 ------ASASISMSFLAFVAFAVSSLISGYNL 201
                 A+++ISM+  AF+A A+S+L SGY L
Sbjct: 157 NIFTDSAASAISMAIFAFIALALSALFSGYKL 182

[Close]

BLAST of Carg18134 vs. TAIR 10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-06
Identity = 34/117 (29.06%), Postives = 47/117 (40.17%), Query Frame = 0


Query: 117 SGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQHMIRHRVRSYFDFFMDQM-- 176
           +  SF  Y E  YC  V +I FVY + Q F  V  +  +  +I   +  Y  F  DQ+  
Sbjct: 89  NSSSFASYPELLYCFGVAVIGFVYTSLQTFKGVCDITHRGVLISEPLSDYISFIFDQVIC 148

Query: 177 ----------------------------ASASISMSFLAFVAFAVSSLISGYNLCSR 204
                                       +  S+SMSF AF+   +S L+SGY LC R
Sbjct: 149 YLLVSSSSVAIAWIQHINEDAIKTLRNNSIVSVSMSFSAFLVLTLSGLLSGYKLCKR 205

The following BLAST results are available for this feature:

BLAST of Carg18134 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021462.1	3.5e-106	100.00	CASP-like protein 4A3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022925737.1	1.0e-89	64.26	CASP-like protein 4A2 [Cucurbita moschata]	[more]
KAG6587474.1	1.3e-89	63.95	CASP-like protein 4A2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023530616.1	1.0e-86	62.38	CASP-like protein 4A2 [Cucurbita pepo subsp. pepo]	[more]
XP_022973205.1	2.8e-79	60.19	CASP-like protein 4A2 [Cucurbita maxima]	[more]

BLAST of Carg18134 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D7LIR2	1.5e-35	42.79	CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT...	[more]
Q84WP5	9.6e-35	42.86	CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1	[more]
Q501G6	8.2e-34	37.33	CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1	[more]
D7MMW4	1.4e-33	36.99	CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT...	[more]
Q9FNE8	2.9e-23	37.50	CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1	[more]

BLAST of Carg18134 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ECH3	4.9e-90	64.26	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433061 PE=3 SV=1	[more]
A0A6J1IAS4	1.3e-79	60.19	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111471736 PE=3 SV=1	[more]
A0A6J1C1D1	2.2e-74	54.83	CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111007480 PE=3 SV=1	[more]
A0A6J1E1W9	5.1e-71	55.49	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430082 PE=3 SV=1	[more]
A0A0A0LNX3	9.6e-70	53.45	CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1	[more]

BLAST of Carg18134 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G36330.1	6.8e-36	42.86	Uncharacterised protein family (UPF0497)	[more]
AT5G62820.1	5.8e-35	37.33	Uncharacterised protein family (UPF0497)	[more]
AT5G40300.1	2.1e-24	37.50	Uncharacterised protein family (UPF0497)	[more]
AT2G38480.1	2.3e-07	30.26	Uncharacterised protein family (UPF0497)	[more]
AT4G11655.1	7.4e-06	29.06	Uncharacterised protein family (UPF0497)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 84..181 e-value: 6.8E-18 score: 64.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..83
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..34
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	PANTHER	PTHR11615:SF147	CASP-LIKE PROTEIN 4A3	coord: 36..173
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 173..204
None	No IPR available	PANTHER	PTHR11615:SF147	CASP-LIKE PROTEIN 4A3	coord: 173..204
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 36..173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18134-RA	Carg18134-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane