Carg17933 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg17933
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionN-acetylglucosaminylphosphatidylinositol deacetylase
LocationCarg_Chr10: 1015358 .. 1016436 (+)
RNA-Seq ExpressionCarg17933
SyntenyCarg17933
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTAAAATAAAGATCGATCTTAGGATCGAAGCCGTTGAATTGAAATCAGACGGAGAAGATGATCAGAGCCAAATTGCGCGGGTGAAAGCCTGTAATCGGCTTCCAGTTTCTCCGTCTATCCGAACAGAGAATCTGAATAACTATTTATTGCATTGAAATTATTATCAAGAATAGCAAATTAAAGGGACGCAATAAATTATTTTTAAATTTTAAATATTTATTAAATACAAAATTAAAAAATTTAGCGGACTCCTGTAAAACAATGGATTTTTTTTTCACCAAATATTAAATTTCCCACCAATCACCGGAGTTGGTTTCCAGTTCCCGCAAAGCAATACACCGTCTCCTTCACGATCTCACACTCTTCTAGCTTCCTCCAGTAGAAACTTCGGAAAATTCCCGGCAAATCGTCGAGAAATTCTCGCCGGAAGTTTCAGAAGTCGCTGAAAGCTCATGCTGTATGCTCCGTAAATTTTCCGGGAAGGAGACGAAGAAAAGAGTGAGTATTCATCAAAGAGAGTAATTTAGCGAATGTTTTCTTATTTGAGTGATTTTTCTCGAATCAGGTTTTTTTTATAGGCATTGAGCTCATTTCCCCTAATCTGTCTCCAATCAATTTTACGTCTCTGAAACTAAGTTGTTAAATCTGTTTATGTCAATTGCGTATTAGCTTTCTTTTTCTCATGCTCTTTAACTCGGATATGACTTTTGAGAATCAGGAAATGGAATTTGATGGATTTTATTGCTTATTGTTTGTTCTCTTTTGGCATTCTCGAGTTAATAATGCATTTATTTGTCTGTAAATGATTTCAGGGGAGTTCTTTGATGGCTTGGCTGTTGGTTATTACTGCCTTCGTTGTAATTTGGATTGCTTCATTATACAAAAATTTTCATGCATCAGTTTCATCTTCCAGGAGTGCTTTCTTAAATGGTGGATCATCACGGATTGTTTTGTCAAATTTTCTTTCTTCTTTAACACTAAAATGCTGTTCTCTGTCATCCTTTGATATCTAGATGGCACTCAAAAGAGAAATATCTTGCTGGTCATTGCCCATCCAGATGATGAGTCTATGTAA

mRNA sequence

ATGCTTAAAATAAAGATCGATCTTAGGATCGAAGCCGTTGAATTGAAATCAGACGGAGAAGATGATCAGAGCCAAATTGCGCGGGTGAAAGCCTTAGAAACTTCGGAAAATTCCCGGCAAATCGTCGAGAAATTCTCGCCGGAAGTTTCAGAAGTCGCTGAAAGCTCATGCTGTATGCTCCGTAAATTTTCCGGGAAGGAGACGAAGAAAAGAGGGAGTTCTTTGATGGCTTGGCTGTTGGTTATTACTGCCTTCGTTGTAATTTGGATTGCTTCATTATACAAAAATTTTCATGCATCAGTTTCATCTTCCAGGAGTGCTTTCTTAAATGATGGCACTCAAAAGAGAAATATCTTGCTGGTCATTGCCCATCCAGATGATGAGTCTATGTAA

Coding sequence (CDS)

ATGCTTAAAATAAAGATCGATCTTAGGATCGAAGCCGTTGAATTGAAATCAGACGGAGAAGATGATCAGAGCCAAATTGCGCGGGTGAAAGCCTTAGAAACTTCGGAAAATTCCCGGCAAATCGTCGAGAAATTCTCGCCGGAAGTTTCAGAAGTCGCTGAAAGCTCATGCTGTATGCTCCGTAAATTTTCCGGGAAGGAGACGAAGAAAAGAGGGAGTTCTTTGATGGCTTGGCTGTTGGTTATTACTGCCTTCGTTGTAATTTGGATTGCTTCATTATACAAAAATTTTCATGCATCAGTTTCATCTTCCAGGAGTGCTTTCTTAAATGATGGCACTCAAAAGAGAAATATCTTGCTGGTCATTGCCCATCCAGATGATGAGTCTATGTAA

Protein sequence

MLKIKIDLRIEAVELKSDGEDDQSQIARVKALETSENSRQIVEKFSPEVSEVAESSCCMLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILLVIAHPDDESM
Homology
[Close]
BLAST of Carg17933 vs. NCBI nr
Match: KAG7023223.1 (hypothetical protein SDJN02_14248, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 246.1 bits (627), Expect = 1.6e-61
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0 

Query: 1   MLKIKIDLRIEAVELKSDGEDDQSQIARVKALETSENSRQIVEKFSPEVSEVAESSCCML 60
           MLKIKIDLRIEAVELKSDGEDDQSQIARVKALETSENSRQIVEKFSPEVSEVAESSCCML
Sbjct: 1   MLKIKIDLRIEAVELKSDGEDDQSQIARVKALETSENSRQIVEKFSPEVSEVAESSCCML 60

Query: 61  RKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILL 120
           RKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILL
Sbjct: 61  RKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILL 120

Query: 121 VIAHPDDESM 131
           VIAHPDDESM
Sbjct: 121 VIAHPDDESM 130

[Close]
BLAST of Carg17933 vs. NCBI nr
Match: KAG6589537.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 242.7 bits (618), Expect = 1.8e-60
Identity = 129/130 (99.23%), Postives = 129/130 (99.23%), Query Frame = 0 

Query: 1   MLKIKIDLRIEAVELKSDGEDDQSQIARVKALETSENSRQIVEKFSPEVSEVAESSCCML 60
           MLKIKIDLRIEAVELKSDGEDDQSQIARVKALETSENSRQIVEKFSPEVSEVAESSCCML
Sbjct: 1   MLKIKIDLRIEAVELKSDGEDDQSQIARVKALETSENSRQIVEKFSPEVSEVAESSCCML 60

Query: 61  RKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILL 120
           RKFSGKETKKRGSSLMAWLLVITAFVVIWIASL KNFHASVSSSRSAFLNDGTQKRNILL
Sbjct: 61  RKFSGKETKKRGSSLMAWLLVITAFVVIWIASLCKNFHASVSSSRSAFLNDGTQKRNILL 120

Query: 121 VIAHPDDESM 131
           VIAHPDDESM
Sbjct: 121 VIAHPDDESM 130

[Close]
BLAST of Carg17933 vs. NCBI nr
Match: XP_022921645.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 139.4 bits (350), Expect = 2.2e-29
Identity = 71/72 (98.61%), Postives = 71/72 (98.61%), Query Frame = 0 

Query: 59  MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNI 118
           MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASL KNFHASVSSSRSAFLNDGTQKRNI
Sbjct: 1   MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLCKNFHASVSSSRSAFLNDGTQKRNI 60

Query: 119 LLVIAHPDDESM 131
           LLVIAHPDDESM
Sbjct: 61  LLVIAHPDDESM 72

[Close]
BLAST of Carg17933 vs. NCBI nr
Match: XP_023515925.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 131.3 bits (329), Expect = 5.9e-27
Identity = 70/73 (95.89%), Postives = 70/73 (95.89%), Query Frame = 0 

Query: 59  MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGT-QKRN 118
           MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASL K FHASVSSSRSAFLNDGT QKRN
Sbjct: 1   MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLCKIFHASVSSSRSAFLNDGTPQKRN 60

Query: 119 ILLVIAHPDDESM 131
           ILLVIAHPDDESM
Sbjct: 61  ILLVIAHPDDESM 73

[Close]
BLAST of Carg17933 vs. NCBI nr
Match: XP_022987980.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-25
Identity = 68/73 (93.15%), Postives = 69/73 (94.52%), Query Frame = 0 

Query: 59  MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGT-QKRN 118
           MLRKFS KETKKRGSSLMAWLLVITAFVVIWIASL K FHASVSSSRSAFLNDGT QK+N
Sbjct: 1   MLRKFSWKETKKRGSSLMAWLLVITAFVVIWIASLCKIFHASVSSSRSAFLNDGTPQKKN 60

Query: 119 ILLVIAHPDDESM 131
           ILLVIAHPDDESM
Sbjct: 61  ILLVIAHPDDESM 73

[Close]
BLAST of Carg17933 vs. ExPASy TrEMBL
Match: A0A6J1E6D6 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita moschata OX=3662 GN=LOC111429838 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.0e-29
Identity = 71/72 (98.61%), Postives = 71/72 (98.61%), Query Frame = 0 

Query: 59  MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNI 118
           MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASL KNFHASVSSSRSAFLNDGTQKRNI
Sbjct: 1   MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLCKNFHASVSSSRSAFLNDGTQKRNI 60

Query: 119 LLVIAHPDDESM 131
           LLVIAHPDDESM
Sbjct: 61  LLVIAHPDDESM 72

[Close]
BLAST of Carg17933 vs. ExPASy TrEMBL
Match: A0A6J1JIB1 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita maxima OX=3661 GN=LOC111485360 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.4e-26
Identity = 68/73 (93.15%), Postives = 69/73 (94.52%), Query Frame = 0 

Query: 59  MLRKFSGKETKKRGSSLMAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGT-QKRN 118
           MLRKFS KETKKRGSSLMAWLLVITAFVVIWIASL K FHASVSSSRSAFLNDGT QK+N
Sbjct: 1   MLRKFSWKETKKRGSSLMAWLLVITAFVVIWIASLCKIFHASVSSSRSAFLNDGTPQKKN 60

Query: 119 ILLVIAHPDDESM 131
           ILLVIAHPDDESM
Sbjct: 61  ILLVIAHPDDESM 73

[Close]
BLAST of Carg17933 vs. ExPASy TrEMBL
Match: A0A6J1C1T3 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3673 GN=LOC111007488 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.6e-16
Identity = 48/56 (85.71%), Postives = 51/56 (91.07%), Query Frame = 0 

Query: 76  MAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLN-DGTQKRNILLVIAHPDDESM 131
           MAWLLVITAFVVIW+ASL K  H SVSSSRSAFLN DGTQKRN+LLV+AHPDDESM
Sbjct: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESM 56

[Close]
BLAST of Carg17933 vs. ExPASy TrEMBL
Match: A0A2P6P8D7 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0203331 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.1e-10
Identity = 37/60 (61.67%), Postives = 46/60 (76.67%), Query Frame = 0 

Query: 76  MAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLND-----GTQKRNILLVIAHPDDESM 131
           MAWLL+I++ +VIWIASL+K  H S S S+ AFLND       +KRN+LLV+AHPDDESM
Sbjct: 1   MAWLLIISSVIVIWIASLFKILHGSYSPSKGAFLNDSNNGGSAKKRNVLLVVAHPDDESM 60

[Close]
BLAST of Carg17933 vs. ExPASy TrEMBL
Match: A0A7N0ZS98 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Kalanchoe fedtschenkoi OX=63787 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.4e-10
Identity = 35/55 (63.64%), Postives = 43/55 (78.18%), Query Frame = 0 

Query: 76  MAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILLVIAHPDDESM 131
           MAWLL++ +  + W+ASL K +HAS SSSR+ FL +   KRNILLVIAHPDDESM
Sbjct: 1   MAWLLIVLSIFLCWLASLCKIYHASYSSSRTTFLTESKHKRNILLVIAHPDDESM 55

[Close]
BLAST of Carg17933 vs. TAIR 10
Match: AT3G58130.1 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.8e-10
Identity = 32/57 (56.14%), Postives = 41/57 (71.93%), Query Frame = 0 

Query: 76  MAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDG--TQKRNILLVIAHPDDESM 131
           MAWL+V  + +VIW+AS  K F  + S S +A L+DG   QK+N+L VIAHPDDESM
Sbjct: 1   MAWLVVSVSLIVIWLASFCKIFFRATSISGAAILDDGKTPQKKNVLFVIAHPDDESM 57

[Close]
BLAST of Carg17933 vs. TAIR 10
Match: AT3G58130.2 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.8e-10
Identity = 32/57 (56.14%), Postives = 41/57 (71.93%), Query Frame = 0 

Query: 76  MAWLLVITAFVVIWIASLYKNFHASVSSSRSAFLNDG--TQKRNILLVIAHPDDESM 131
           MAWL+V  + +VIW+AS  K F  + S S +A L+DG   QK+N+L VIAHPDDESM
Sbjct: 1   MAWLVVSVSLIVIWLASFCKIFFRATSISGAAILDDGKTPQKKNVLFVIAHPDDESM 57

[Close]
BLAST of Carg17933 vs. TAIR 10
Match: AT2G27340.2 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.8e-08
Identity = 26/52 (50.00%), Postives = 38/52 (73.08%), Query Frame = 0 

Query: 79  LLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILLVIAHPDDESM 131
           ++ ++  VVIW+AS +K F  + S SR+  L+DG  K+N++ VIAHPDDESM
Sbjct: 3   VVFLSLIVVIWVASFFKIFFRATSISRATILDDG--KKNVMFVIAHPDDESM 52

[Close]
BLAST of Carg17933 vs. TAIR 10
Match: AT2G27340.3 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.8e-08
Identity = 26/52 (50.00%), Postives = 38/52 (73.08%), Query Frame = 0 

Query: 79  LLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILLVIAHPDDESM 131
           ++ ++  VVIW+AS +K F  + S SR+  L+DG  K+N++ VIAHPDDESM
Sbjct: 3   VVFLSLIVVIWVASFFKIFFRATSISRATILDDG--KKNVMFVIAHPDDESM 52

[Close]
BLAST of Carg17933 vs. TAIR 10
Match: AT2G27340.4 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.8e-08
Identity = 26/52 (50.00%), Postives = 38/52 (73.08%), Query Frame = 0 

Query: 79  LLVITAFVVIWIASLYKNFHASVSSSRSAFLNDGTQKRNILLVIAHPDDESM 131
           ++ ++  VVIW+AS +K F  + S SR+  L+DG  K+N++ VIAHPDDESM
Sbjct: 3   VVFLSLIVVIWVASFFKIFFRATSISRATILDDG--KKNVMFVIAHPDDESM 52

The following BLAST results are available for this feature:
BLAST of Carg17933 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7023223.11.6e-61100.00hypothetical protein SDJN02_14248, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6589537.11.8e-6099.23N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, partial [Cucurbita arg... [more]
XP_022921645.12.2e-2998.61N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurb... [more]
XP_023515925.15.9e-2795.89N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurb... [more]
XP_022987980.11.1e-2593.15N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurb... [more]
BLAST of Carg17933 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Carg17933 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1E6D61.0e-2998.61N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita moschata OX=36... [more]
A0A6J1JIB15.4e-2693.15N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita maxima OX=3661... [more]
A0A6J1C1T36.6e-1685.71N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3... [more]
A0A2P6P8D71.1e-1061.67N-acetylglucosaminylphosphatidylinositol deacetylase OS=Rosa chinensis OX=74649 ... [more]
A0A7N0ZS982.4e-1063.64N-acetylglucosaminylphosphatidylinositol deacetylase OS=Kalanchoe fedtschenkoi O... [more]
BLAST of Carg17933 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G58130.11.8e-1056.14N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT3G58130.21.8e-1056.14N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT2G27340.23.8e-0850.00N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT2G27340.33.8e-0850.00N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT2G27340.43.8e-0850.00N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg17933-RACarg17933-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000225 N-acetylglucosaminylphosphatidylinositol deacetylase activity