Homology
BLAST of Carg17822 vs. NCBI nr
Match:
KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 982.2 bits (2538), Expect = 1.6e-282
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG
Sbjct: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of Carg17822 vs. NCBI nr
Match:
XP_022960871.1 (beta-amyrin 28-oxidase [Cucurbita moschata])
HSP 1 Score: 972.2 bits (2512), Expect = 1.6e-279
Identity = 479/483 (99.17%), Postives = 479/483 (99.17%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
FIV YLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTT 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of Carg17822 vs. NCBI nr
Match:
XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 966.1 bits (2496), Expect = 1.1e-277
Identity = 474/483 (98.14%), Postives = 477/483 (98.76%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
MADFLISLLILFLS ISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE
Sbjct: 1 MADFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLFVSLEDRIHVERLSEPFQ+IAAGIISMPIDLPGTPFNRAIKASNFVRKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
FIVKYLAELPQIYD VYREQMEIAKAK AGELLKWEDIQKMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVKYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFRE IN FVFNGFYIPKGWK+YWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKL+DKEKIIVDPLPIPEKGLPVRLFPHKY
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPHKY 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of Carg17822 vs. NCBI nr
Match:
XP_022987538.1 (beta-amyrin 28-oxidase [Cucurbita maxima])
HSP 1 Score: 945.7 bits (2443), Expect = 1.6e-271
Identity = 468/485 (96.49%), Postives = 472/485 (97.32%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
M DFLISLLILFLS ISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE
Sbjct: 1 MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGP CNKFLFSNENKLVTSWWPDSVNKIFPA
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENRE VLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLF+SLEDRIHVERLSEPFQ+IAAGIISMPIDLPGTPFNRAIKASNFVRKELLS
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEI--AKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPL 360
FIVKYLAELPQIYD VYREQMEI AKAKA GELLKWEDIQKMKYSWNV CEVLRLASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360
Query: 361 QGAFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420
QGAFREAIN FVFNGF IPKGWK+YWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420
Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPH 480
PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+KEKIIVDPLPIPEKGLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480
Query: 481 KYLSL 484
K LSL
Sbjct: 481 KTLSL 485
BLAST of Carg17822 vs. NCBI nr
Match:
XP_022158061.1 (beta-amyrin 28-oxidase [Momordica charantia])
HSP 1 Score: 853.6 bits (2204), Expect = 8.3e-244
Identity = 407/482 (84.44%), Postives = 438/482 (90.87%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
M FL+SLLI F+S++ + LF+LFYKHKS FSYPN PPG IG PIIGESLEF STGWKGE
Sbjct: 1 MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDR+NKYKS IFKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFP+
Sbjct: 61 PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
ST+ +S E A +LKKLLP+FLKPEAL RYIG+MD LA RHFASSWEN++QVLVFPLAKSF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLFVS+ED HVERLS PFQ IAAGIISMPID PGTPFNR IKASNF+RKELLS
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IV+QRK DLAEGKASPTQDILSHMLLTCDE G YMNE IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
FIVK+LAELP IYDGVY+EQM IAKAKA GELL WEDIQKMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREAIN FVFNGFYIPKGWK+YWSSNSTHKN YFP+PY FDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+L+KEKI+VDP+PIP+KGLPVRLFPHK
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
Query: 481 LS 483
+S
Sbjct: 481 VS 482
BLAST of Carg17822 vs. ExPASy Swiss-Prot
Match:
Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)
HSP 1 Score: 733.0 bits (1891), Expect = 2.1e-210
Identity = 347/478 (72.59%), Postives = 404/478 (84.52%), Query Frame = 0
Query: 3 DFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPE 62
+F +SLL+LF++ ISL+LF +FYK KS N PPG +G PIIGESLEFLSTGWKG PE
Sbjct: 4 NFYLSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPE 63
Query: 63 KFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP-AS 122
KFIFDR+ KY S++FKTSIVG + CG A NKFLFSNENKLVT+WWPDSVNKIFP S
Sbjct: 64 KFIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTS 123
Query: 123 TEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFT 182
+ N KE +I+++KLLP+F KPEALQRY+GVMD +AQRHF + W+N+ ++ V+PLAK +T
Sbjct: 124 LDSNLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYT 183
Query: 183 FSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSI 242
F LACRLF+S+ED HV + S+PFQ IAAGIIS+PIDLPGTPFN+AIKASNF+RKEL+ I
Sbjct: 184 FLLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKI 243
Query: 243 VRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTF 302
++QR+ DLAEG ASPTQDILSHMLLT DE G+ MNE IAD ILGLLIGGHDT S ACTF
Sbjct: 244 IKQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTF 303
Query: 303 IVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGA 362
+VKYL ELP IYD VY+EQMEIAK+K AGELL W+D++KMKYSWNV CEV+RL+ PLQG
Sbjct: 304 LVKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGG 363
Query: 363 FREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFG 422
FREAI F+FNGF IPKGWK+YWS+NSTHKN FP P KFDP+RFEGNGPAPYTFVPFG
Sbjct: 364 FREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFG 423
Query: 423 GGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHK 480
GGPRMCPGKEYA+LEILVFMHNLVKRFKW K++ EKIIVDP PIP K LP+RL+PHK
Sbjct: 424 GGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHK 478
BLAST of Carg17822 vs. ExPASy Swiss-Prot
Match:
A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)
HSP 1 Score: 731.9 bits (1888), Expect = 4.8e-210
Identity = 341/479 (71.19%), Postives = 411/479 (85.80%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
M F +SLL++ + +S+ L++L YKH+S F+ PN PPG IG P++GESLEFLSTGWKG
Sbjct: 1 MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDR++KY S++FKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FP+
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
ST+ +SKE A +++KLLP+FLKPEALQRY G+MD +AQRHFA SW+NR++V+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TF LACRLF+S+ED HV + +PF +A+G+I++PIDLPGTPF+RAIKASNF+RKEL +
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
I++QRK DLAE KAS TQDILSHMLL DE G +MNE +IAD ILGLLIGGHDT S+A T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
F+VKY+AELP IY+ VY+EQMEIA +KA GELL W+D+QKM+YSWNV CEV+RLA PLQG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREAI FVFNGF IPKGWK+YWS+NSTHK+ FP+P KFDP+RFEG GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHK 480
GGGPRMCPGKEYA+LEIL+FMHNLVKRFKW KLL EKIIVDP+P+P KGLPVRL PHK
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPHK 479
BLAST of Carg17822 vs. ExPASy Swiss-Prot
Match:
F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)
HSP 1 Score: 731.1 bits (1886), Expect = 8.2e-210
Identity = 341/479 (71.19%), Postives = 409/479 (85.39%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
M F +SLL++F+ +S+ L +LFYKH+S F+ PN PPG IG P++GESLEFLSTGWKG
Sbjct: 1 MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDR++KY S++FKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FP+
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
ST+ +SKE A +++KLLP+F KPEALQRYIG+MD +AQRHFA SW+NR++V+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TF LACRLF+S+ED HV + +PF +A+G+I++PIDLPGTPF+RAIKASNF+RKEL +
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
I++QRK DLAEGKAS QDILSHMLL DE G +MNE IAD ILGLLIGGHDT SAA T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
F++KY+AELP IY+ VY EQMEIA +KA GELL W+D+QKM+YSWNV CEV+RLA PLQG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREAI FVFNGF IPKGWK+YWS+NSTHK+ FP+P FDP+RFEG+GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHK 480
GGGPRMCPGKEYA+LEILVFMHN+VKRFKW KLL EKIIVDP+P+P KGLPVRL P K
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPRK 479
BLAST of Carg17822 vs. ExPASy Swiss-Prot
Match:
A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)
HSP 1 Score: 727.6 bits (1877), Expect = 9.0e-209
Identity = 342/477 (71.70%), Postives = 406/477 (85.12%), Query Frame = 0
Query: 4 FLISLLILFLSLISLTLFVLFYKHKS-LFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPE 63
F + LL LF+ +SL+ + LFYK KS S PPG G P+IGES EFLSTGWKG PE
Sbjct: 4 FYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKGYPE 63
Query: 64 KFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPAST 123
KFIFDRL KY S++FKTS+ G PAA FCG ACNKFLFSNENKLV +WWPDSVNK+FP+ST
Sbjct: 64 KFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSST 123
Query: 124 EINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTF 183
+ +SKE AI+++KLLP FLKPEALQRY+GVMD++A +HF S WEN+++VLVFPLAK++TF
Sbjct: 124 QTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKNYTF 183
Query: 184 SLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIV 243
+AC++FVS+E+ V +L EPF +IA+GIIS+PIDLPGTPFN AIK+S VR +L+ I+
Sbjct: 184 WIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLMGII 243
Query: 244 RQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFI 303
+QRK+DL EGKASPTQDILSHMLLT DE G++M E IAD ILGLLIGGHDT S+ACTF+
Sbjct: 244 KQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSACTFV 303
Query: 304 VKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGAF 363
VK+LAELP+IY+GVY+EQMEIAK+K AGELL WEDIQKMKYSWNV CEVLRLA PLQGAF
Sbjct: 304 VKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAF 363
Query: 364 REAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFGG 423
REA+ F +NGF IPKGWK+YWS+NSTH+N FPEP KFDPSRFEG GP PY+FVPFGG
Sbjct: 364 REALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVPFGG 423
Query: 424 GPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHK 480
GPRMCPGKEYA+LEILVFMH++VKRFKW K++ EKI+V+P+PIP KGLPVRLFPHK
Sbjct: 424 GPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480
BLAST of Carg17822 vs. ExPASy Swiss-Prot
Match:
I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)
HSP 1 Score: 717.2 bits (1850), Expect = 1.2e-205
Identity = 340/478 (71.13%), Postives = 401/478 (83.89%), Query Frame = 0
Query: 4 FLISLLILFLSLISLTLFVLFYKHK--SLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEP 63
F + LL LF+ ISL+ LFYK K S +P PPG G PIIGES EFLSTGWKG P
Sbjct: 4 FYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFP-LPPGKTGWPIIGESYEFLSTGWKGYP 63
Query: 64 EKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPAS 123
EKFIFDR+ KY S++FKTSI G PAA+FCG ACNKFLFSNENKLV +WWPDSVNK+FP+S
Sbjct: 64 EKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSS 123
Query: 124 TEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFT 183
T+ +SKE AI+++K+LP F KPEALQRYIG+MD++A HF S WEN+ +V+VFPLAKS+T
Sbjct: 124 TQTSSKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAKSYT 183
Query: 184 FSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSI 243
F +AC++FVS+E+ V L EPF +IA+GIIS+PIDLPGTPFN AIK+S VR++L+ I
Sbjct: 184 FWIACKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKLVGI 243
Query: 244 VRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTF 303
++QRK DL EGKAS TQDILSHMLLT DE G++M E IAD ILGLLIGGHDT S+ACTF
Sbjct: 244 IKQRKIDLGEGKASATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSACTF 303
Query: 304 IVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGA 363
+VK+LAELPQIY+GVY+EQMEI K+K AGELLKWEDIQKMKYSWNV CEVLRLA PLQGA
Sbjct: 304 VVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPLQGA 363
Query: 364 FREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFG 423
FREA++ F +NGF IPKGWK+YWS+NSTH N FPEP KFDPSRF+G GP P++FVPFG
Sbjct: 364 FREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDGAGPPPFSFVPFG 423
Query: 424 GGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHK 480
GGPRMCPGKEYA+LEILVFMH+LVKRFKW K++ EKI+V+P+PIP GLPVRLFPHK
Sbjct: 424 GGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPHK 480
BLAST of Carg17822 vs. ExPASy TrEMBL
Match:
A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)
HSP 1 Score: 972.2 bits (2512), Expect = 7.8e-280
Identity = 479/483 (99.17%), Postives = 479/483 (99.17%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
FIV YLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTT 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of Carg17822 vs. ExPASy TrEMBL
Match:
A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)
HSP 1 Score: 945.7 bits (2443), Expect = 7.8e-272
Identity = 468/485 (96.49%), Postives = 472/485 (97.32%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
M DFLISLLILFLS ISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE
Sbjct: 1 MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGP CNKFLFSNENKLVTSWWPDSVNKIFPA
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENRE VLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLF+SLEDRIHVERLSEPFQ+IAAGIISMPIDLPGTPFNRAIKASNFVRKELLS
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDT SAACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEI--AKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPL 360
FIVKYLAELPQIYD VYREQMEI AKAKA GELLKWEDIQKMKYSWNV CEVLRLASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360
Query: 361 QGAFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420
QGAFREAIN FVFNGF IPKGWK+YWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420
Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPH 480
PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+KEKIIVDPLPIPEKGLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480
Query: 481 KYLSL 484
K LSL
Sbjct: 481 KTLSL 485
BLAST of Carg17822 vs. ExPASy TrEMBL
Match:
A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)
HSP 1 Score: 853.6 bits (2204), Expect = 4.0e-244
Identity = 407/482 (84.44%), Postives = 438/482 (90.87%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
M FL+SLLI F+S++ + LF+LFYKHKS FSYPN PPG IG PIIGESLEF STGWKGE
Sbjct: 1 MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDR+NKYKS IFKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFP+
Sbjct: 61 PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
ST+ +S E A +LKKLLP+FLKPEAL RYIG+MD LA RHFASSWEN++QVLVFPLAKSF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLFVS+ED HVERLS PFQ IAAGIISMPID PGTPFNR IKASNF+RKELLS
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IV+QRK DLAEGKASPTQDILSHMLLTCDE G YMNE IAD ILGLLIGGHDT SAACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
FIVK+LAELP IYDGVY+EQM IAKAKA GELL WEDIQKMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREAIN FVFNGFYIPKGWK+YWSSNSTHKN YFP+PY FDPSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+L+KEKI+VDP+PIP+KGLPVRLFPHK
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
Query: 481 LS 483
+S
Sbjct: 481 VS 482
BLAST of Carg17822 vs. ExPASy TrEMBL
Match:
A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)
HSP 1 Score: 843.6 bits (2178), Expect = 4.2e-241
Identity = 403/483 (83.44%), Postives = 440/483 (91.10%), Query Frame = 0
Query: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
M FLISLLIL +SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GESLEFLS+GW+G
Sbjct: 1 MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
Query: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
+T+ +SKE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S W+NRE+VLVFPLAKSF
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
TFSLACRLF+S+ED IHVERLS PF+ IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
IVRQRK DLAEGKA TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDT S ACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
FIVK+LAELP IYD VY EQMEIA+AKA GE LKWEDI+KMKYSWNV CEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
AFREA+N FVFNGF+IPKGWK+YWS+NSTHKN YFPEPYKF+P RFEGNGP PYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+ E IIV+P+PIP+KGLPVRLFPH+
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of Carg17822 vs. ExPASy TrEMBL
Match:
A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)
HSP 1 Score: 839.3 bits (2167), Expect = 7.9e-240
Identity = 399/480 (83.12%), Postives = 439/480 (91.46%), Query Frame = 0
Query: 4 FLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEK 63
FLISLLIL +SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GES+EFLS+GWKG PEK
Sbjct: 4 FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPEK 63
Query: 64 FIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPASTE 123
FIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFP++T+
Sbjct: 64 FIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQ 123
Query: 124 INSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFS 183
++KE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S W+NRE+VLVFPLAKSFTFS
Sbjct: 124 TSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTFS 183
Query: 184 LACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVR 243
+ACRLF+S+ED IHVERLS PF+ IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++IVR
Sbjct: 184 IACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVR 243
Query: 244 QRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIV 303
QRK DLAEGKA TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDT S ACTFIV
Sbjct: 244 QRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIV 303
Query: 304 KYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGAFR 363
K+LAELP IYD VY EQMEIA+AKA GE LKWEDI+KMKYSWNV CEVLR+ASPLQGAFR
Sbjct: 304 KFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFR 363
Query: 364 EAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFGGG 423
EA++ FVFNGF+IPKGWK+YWS+NSTHKN YFPEPYKFDP RFEGNGP PYTFVPFGGG
Sbjct: 364 EALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPFGGG 423
Query: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKYLSL 483
PRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+ E IIV+P+PIP+KGLPVRLFPH+ LSL
Sbjct: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQPLSL 483
BLAST of Carg17822 vs. TAIR 10
Match:
AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )
HSP 1 Score: 625.9 bits (1613), Expect = 2.6e-179
Identity = 299/478 (62.55%), Postives = 369/478 (77.20%), Query Frame = 0
Query: 4 FLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEK 63
++ ++ILFLS I L+L +L KH S FSYPN PPG GLP+IGES FLS G +G PEK
Sbjct: 2 YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEK 61
Query: 64 FIFDRLNKYKSD---IFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 123
FI DR+ ++ S +FKT + G P A+ G + NKFLF+NENKLV SWWPDSVNKIFP+
Sbjct: 62 FITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPS 121
Query: 124 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 183
S + +SKE A +L+ LL +F+KPEAL+RY+GVMDE+AQRHF + W N++QV+VFPL K F
Sbjct: 122 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKF 181
Query: 184 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 243
TFS+ACR F+S+ED V +L E F ++A GI S+PIDLPGT FNRAIKAS +RKE+ +
Sbjct: 182 TFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSA 241
Query: 244 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 303
IVRQRK +L GKA DILSHML+ IG+ +E +AD I+GLLIGGHDT S CT
Sbjct: 242 IVRQRKEELKAGKALEEHDILSHMLM---NIGETKDED-LADKIIGLLIGGHDTASIVCT 301
Query: 304 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 363
F+V YLAE P +Y V +EQ EI K K E L+WEDI+KM+YSWNV CEV+R+ PL G
Sbjct: 302 FVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSG 361
Query: 364 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 423
FREAI+HF F GFYIPKGWK+YWS+ +TH N YFPEP +F+P+RFEG+GP PYT+VPF
Sbjct: 362 TFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPF 421
Query: 424 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKE-KIIVDPLPIPEKGLPVRLFP 478
GGGPRMCPGKEYA+LEIL+FMHNLV RFKW K+ E KI+VDPLPIP+KGLP+R+FP
Sbjct: 422 GGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 475
BLAST of Carg17822 vs. TAIR 10
Match:
AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )
HSP 1 Score: 365.5 bits (937), Expect = 6.4e-101
Identity = 181/318 (56.92%), Postives = 232/318 (72.96%), Query Frame = 0
Query: 6 ISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEKFI 65
++++ LF+S I L KH S F YPN PPG IG P+IGE+L FLS G +G PEKF+
Sbjct: 3 LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62
Query: 66 FDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPASTEIN 125
DR+ + S IFKT + G P A+ G + NKFLF+NENKLV SWWPDSVNKIFP+ST+ +
Sbjct: 63 TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122
Query: 126 SKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFSLA 185
SKE AI+ + LL +KPEAL+RY+GVMDE+AQ+HF + W N++Q++VFPL K FTFS+A
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182
Query: 186 CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 245
CRLF+S++D V +L EPF ++ G+ S+PIDLPGT FNRAIKAS + KE+ +I+RQR
Sbjct: 183 CRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQR 242
Query: 246 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIVKY 305
K +L GK S QDILSHML+ IG+ +E +AD I+ LLIGGHDTTS CTF+V Y
Sbjct: 243 KEELKAGKVSVEQDILSHMLM---NIGETKDED-LADKIIALLIGGHDTTSIVCTFVVNY 302
Query: 306 LAELPQIYDGVYREQMEI 324
LAE P IY V E M+I
Sbjct: 303 LAEFPHIYQRVL-EGMQI 315
BLAST of Carg17822 vs. TAIR 10
Match:
AT2G42850.1 (cytochrome P450, family 718 )
HSP 1 Score: 273.5 bits (698), Expect = 3.3e-73
Identity = 163/484 (33.68%), Postives = 261/484 (53.93%), Query Frame = 0
Query: 3 DFLISLLILFL-SLISLTLFVLFYKH-----KSLFSYPNTPPGAIGLPIIGESLEFLSTG 62
+F++S + L + + IS TLF KH K + PG +GLP IGE+++F
Sbjct: 6 NFVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQ 65
Query: 63 WKGEP-EKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVN 122
E F+ R+ K+ +IFKT I+G P + G N+ + SNE LV S WP S
Sbjct: 66 KSNRVFEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSV 125
Query: 123 KIFPASTEINSKESAIR-LKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVF 182
++ + + + R L+ ++ L L+ I + + + H + W +E++ ++
Sbjct: 126 QLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLY 185
Query: 183 PLAKSFTFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFV 242
AK TF++ ++ I + E F+ + G+ ++P++ P + F RA KA +
Sbjct: 186 RSAKVLTFTVVFECLYGIKVEI---GMLEVFERVLEGVFALPVEFPCSKFARAKKARLEI 245
Query: 243 RKELLSIVRQRKSDL-AEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHD 302
L+ VR+++ ++ EG P + S L + I + E + DN++ L+ HD
Sbjct: 246 ETFLVGKVREKRREMEKEGAEKPNTTLFSR--LVEELIKGVITEEEVVDNMVLLVFAAHD 305
Query: 303 TTSAACTFIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLR 362
TTS A + K LA+ P D + +E +I K GE L ED++KMKYSW V E +R
Sbjct: 306 TTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMR 365
Query: 363 LASPLQGAFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPA 422
L+ P+ G+FR+A+ + G+ IPKGWK+ W++ TH N F +P FDP+RF+ A
Sbjct: 366 LSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKPIQA 425
Query: 423 PYTFVPFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPV 478
YT++PFGGGPR+C G + AK+ ILVFMH +V F W+ + E I +DPLP P G+P+
Sbjct: 426 -YTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPI 482
BLAST of Carg17822 vs. TAIR 10
Match:
AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 267.3 bits (682), Expect = 2.4e-71
Identity = 165/476 (34.66%), Postives = 255/476 (53.57%), Query Frame = 0
Query: 5 LISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEKF 64
LI+L I+ + ++ L + L +K + L PPG++GLP IGE+L + P F
Sbjct: 22 LITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENPNSF 81
Query: 65 IFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNK-IFPASTE 124
R NKY DIFKT I+G P + P + + ++ L +P S + I P +
Sbjct: 82 FATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALF 141
Query: 125 INSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFS 184
+ LK+L+ P AL+ + + EL SSW +++ + K + F
Sbjct: 142 FHQGPYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFD 201
Query: 185 LA-CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIV 244
+A F E+ ++ + +Q + G SMP+DLPGT F++++KA + +EL ++
Sbjct: 202 VAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI 261
Query: 245 RQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFI 304
+R+ + E +L +L D+ +++S IADNI+G++ DTT++ T++
Sbjct: 262 EKRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWL 321
Query: 305 VKYLAELPQIYDGVYREQMEI-AKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGA 364
+KYL + P + V REQ I K K + WED +KM + V E LR AS L
Sbjct: 322 LKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFT 381
Query: 365 FREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFG 424
FREA+ ++G+ IPKGWKV H + +FP+P KFDPSRFE P PYT++PFG
Sbjct: 382 FREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFG 441
Query: 425 GGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFP 478
G CPG E AKLE+L+ +H+L F+W + D+E I P P+P+KGLP+R+ P
Sbjct: 442 NGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481
BLAST of Carg17822 vs. TAIR 10
Match:
AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 267.3 bits (682), Expect = 2.4e-71
Identity = 165/476 (34.66%), Postives = 255/476 (53.57%), Query Frame = 0
Query: 5 LISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEKF 64
LI+L I+ + ++ L + L +K + L PPG++GLP IGE+L + P F
Sbjct: 22 LITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENPNSF 81
Query: 65 IFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNK-IFPASTE 124
R NKY DIFKT I+G P + P + + ++ L +P S + I P +
Sbjct: 82 FATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALF 141
Query: 125 INSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFS 184
+ LK+L+ P AL+ + + EL SSW +++ + K + F
Sbjct: 142 FHQGPYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFD 201
Query: 185 LA-CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIV 244
+A F E+ ++ + +Q + G SMP+DLPGT F++++KA + +EL ++
Sbjct: 202 VAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI 261
Query: 245 RQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFI 304
+R+ + E +L +L D+ +++S IADNI+G++ DTT++ T++
Sbjct: 262 EKRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWL 321
Query: 305 VKYLAELPQIYDGVYREQMEI-AKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGA 364
+KYL + P + V REQ I K K + WED +KM + V E LR AS L
Sbjct: 322 LKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFT 381
Query: 365 FREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFG 424
FREA+ ++G+ IPKGWKV H + +FP+P KFDPSRFE P PYT++PFG
Sbjct: 382 FREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFG 441
Query: 425 GGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFP 478
G CPG E AKLE+L+ +H+L F+W + D+E I P P+P+KGLP+R+ P
Sbjct: 442 NGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q2MJ20 | 2.1e-210 | 72.59 | Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV... | [more] |
A5BFI4 | 4.8e-210 | 71.19 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2 | [more] |
F6H9N6 | 8.2e-210 | 71.19 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1 | [more] |
A0A0S2II38 | 9.0e-209 | 71.70 | Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... | [more] |
I7C6E8 | 1.2e-205 | 71.13 | Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1H8T4 | 7.8e-280 | 99.17 | beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1 | [more] |
A0A6J1JJQ7 | 7.8e-272 | 96.49 | beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1 | [more] |
A0A6J1DZX7 | 4.0e-244 | 84.44 | beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1 | [more] |
A0A1S3B8H4 | 4.2e-241 | 83.44 | beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1 | [more] |
A0A0A0LXN3 | 7.9e-240 | 83.13 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT5G36110.1 | 2.6e-179 | 62.55 | cytochrome P450, family 716, subfamily A, polypeptide 1 | [more] |
AT5G36140.1 | 6.4e-101 | 56.92 | cytochrome P450, family 716, subfamily A, polypeptide 2 | [more] |
AT2G42850.1 | 3.3e-73 | 33.68 | cytochrome P450, family 718 | [more] |
AT2G29090.1 | 2.4e-71 | 34.66 | cytochrome P450, family 707, subfamily A, polypeptide 2 | [more] |
AT2G29090.2 | 2.4e-71 | 34.66 | cytochrome P450, family 707, subfamily A, polypeptide 2 | [more] |