Carg17796 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17796
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	4-hydroxy-4-methyl-2-oxoglutarate aldolase
Location	Carg_Chr10: 3981713 .. 3982213 (-)
RNA-Seq Expression	Carg17796
Synteny	Carg17796

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTGGTTACCACTGCGGAAGTTTGTGATGCTAATCCACAGCTCATTGTGAATGGTGAACTTCGAGCCCTCGAACCAGTTTTCCAGATATATGGTCGACGCCAAGTCTTTTCAGGACCAGTAGTCACACTGAAGGTATTTGAAGATAACGTCTTGATTCGTGAGTTCCTTGAGGAGAAGGGTAACGGCCGAGTTCTTGTCGTGGACGGGGGCGGTAGTAAGAGATGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAAAACAATGGATGGGCTGGTATAGTTGTGAATGGATGTGTTAGAGATGTGGATGAAATTAACGGATGCGATATCGGGGTACGGGCTCTCGCTTCACATCCAATGAAAGCCTACAAAAAACGGGTTGGAGAAAAGCACGTCCCGATAACTATAGCTGGGACAAGGATCATGGATGGAGAATGGCTGTATGCAGATACTGATGGGATTCTGGTTTCACGAACCGAGTTGTCCGTTTAG

mRNA sequence

ATGGCCTTGGTTACCACTGCGGAAGTTTGTGATGCTAATCCACAGCTCATTGTGAATGGTGAACTTCGAGCCCTCGAACCAGTTTTCCAGATATATGGTCGACGCCAAGTCTTTTCAGGACCAGTAGTCACACTGAAGGTATTTGAAGATAACGTCTTGATTCGTGAGTTCCTTGAGGAGAAGGGTAACGGCCGAGTTCTTGTCGTGGACGGGGGCGGTAGTAAGAGATGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAAAACAATGGATGGGCTGGTATAGTTGTGAATGGATGTGTTAGAGATGTGGATGAAATTAACGGATGCGATATCGGGGTACGGGCTCTCGCTTCACATCCAATGAAAGCCTACAAAAAACGGGTTGGAGAAAAGCACGTCCCGATAACTATAGCTGGGACAAGGATCATGGATGGAGAATGGCTGTATGCAGATACTGATGGGATTCTGGTTTCACGAACCGAGTTGTCCGTTTAG

Coding sequence (CDS)

ATGGCCTTGGTTACCACTGCGGAAGTTTGTGATGCTAATCCACAGCTCATTGTGAATGGTGAACTTCGAGCCCTCGAACCAGTTTTCCAGATATATGGTCGACGCCAAGTCTTTTCAGGACCAGTAGTCACACTGAAGGTATTTGAAGATAACGTCTTGATTCGTGAGTTCCTTGAGGAGAAGGGTAACGGCCGAGTTCTTGTCGTGGACGGGGGCGGTAGTAAGAGATGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAAAACAATGGATGGGCTGGTATAGTTGTGAATGGATGTGTTAGAGATGTGGATGAAATTAACGGATGCGATATCGGGGTACGGGCTCTCGCTTCACATCCAATGAAAGCCTACAAAAAACGGGTTGGAGAAAAGCACGTCCCGATAACTATAGCTGGGACAAGGATCATGGATGGAGAATGGCTGTATGCAGATACTGATGGGATTCTGGTTTCACGAACCGAGTTGTCCGTTTAG

Protein sequence

MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEEKGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV

Homology

[Close]

BLAST of Carg17796 vs. NCBI nr
Match: XP_022960655.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita moschata] >XP_022960656.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita moschata] >KAG7023765.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.6 bits (867), Expect = 3.1e-89
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. NCBI nr
Match: XP_022988625.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita maxima] >XP_022988626.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita maxima])

HSP 1 Score: 336.7 bits (862), Expect = 1.2e-88
Identity = 165/166 (99.40%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. NCBI nr
Match: XP_008443692.1 (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo] >XP_038880156.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Benincasa hispida] >KAA0038259.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo var. makuwa] >TYK30658.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 335.9 bits (860), Expect = 2.0e-88
Identity = 163/166 (98.19%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEP+FQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPIT+AGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. NCBI nr
Match: XP_023515582.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita pepo subsp. pepo] >XP_023515583.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.5 bits (859), Expect = 2.6e-88
Identity = 164/166 (98.80%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGW+GIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWSGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. NCBI nr
Match: XP_004139103.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis sativus] >KGN66670.1 hypothetical protein Csa_007309 [Cucumis sativus])

HSP 1 Score: 334.7 bits (857), Expect = 4.5e-88
Identity = 162/166 (97.59%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEP+FQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGW+GIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWSGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPIT+AGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy Swiss-Prot
Match: Q9FFE0 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana OX=3702 GN=At5g16450 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 3.9e-79
Identity = 140/166 (84.34%), Postives = 156/166 (93.98%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MA VTTAEVCDAN +LI +G+LRAL+P+FQIYGRRQ+FSGPVVT+KVFEDN LIR+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HP+KA KK +GE+ VP+ IAGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy Swiss-Prot
Match: Q9M8R9 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g02770 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 1.7e-77
Identity = 139/166 (83.73%), Postives = 153/166 (92.17%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MA VTTAEVCDAN ++I +G+LRAL+PVFQIYGRRQ+FSGPVVT+KVFEDN LIR FLEE
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGI+VNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HP+KA KK +GE+ V + IAGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy Swiss-Prot
Match: Q9FH13 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana OX=3702 GN=At5g56260 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 4.2e-65
Identity = 117/166 (70.48%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MA   TAE CD+N +LI NG+LRAL P+F+IYG+R+ FSGP+VTLKVFEDNVL+R  LE 
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KG G VLV+DGGGS RCA++GGN    AQNNGW+GIVVNGCVRDVDEIN CD+GVRAL S
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           +P+K+ KK  GEK+VP+ I GT I DGEWLYAD+DGIL+S+TELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy Swiss-Prot
Match: Q46ZV6 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=Reut_A1963 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 8.6e-50
Identity = 86/159 (54.09%), Postives = 116/159 (72.96%), Query Frame = 0


Query: 6   TAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEEKGNGR 65
           T ++CDAN   + +G LR + PVF+ +G++Q F+GP  TLK+FEDN L+RE LE  G GR
Sbjct: 5   TTDLCDANEPRLADGTLRVMAPVFRAFGKQQAFAGPAATLKIFEDNSLVREALESPGQGR 64

Query: 66  VLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKA 125
           VLVVDGGGS RCA++GGN  + A+ NGW GI+VNGCVRD  E++ CDIG+RALA+HP K+
Sbjct: 65  VLVVDGGGSLRCALVGGNLGLLAEKNGWVGIIVNGCVRDTGELDVCDIGIRALAAHPQKS 124

Query: 126 YKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTEL 165
            K+  GE+ V + + G  +  G W+Y D DG+LVS  +L
Sbjct: 125 QKRGAGERDVAVQMPGAVVRPGNWIYVDADGVLVSEEKL 163

[Close]

BLAST of Carg17796 vs. ExPASy Swiss-Prot
Match: A9AHQ6 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=Bmul_1223 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 7.2e-49
Identity = 90/160 (56.25%), Postives = 114/160 (71.25%), Query Frame = 0


Query: 6   TAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEEKGNGR 65
           T ++CDA+   +  G LR L+P  + YG    F+GP  TLKVFEDN L+R  LE+ G GR
Sbjct: 5   TTDLCDAHEDKLAAGTLRVLQPALRPYGGAARFAGPAATLKVFEDNTLVRAALEQDGGGR 64

Query: 66  VLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKA 125
           VLVVDGG S RCA++GGN    A+ NGWAGIVV+GCVRD  E+  C +GV ALA+HP K+
Sbjct: 65  VLVVDGGASLRCALVGGNLGALAEKNGWAGIVVHGCVRDAAELRECKVGVLALATHPRKS 124

Query: 126 YKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELS 166
            K+  GE+ VP+T+ GTRI  GEW+YAD DG+LVS T L+
Sbjct: 125 DKRGAGERDVPVTVLGTRIAPGEWIYADDDGVLVSATSLT 164

[Close]

BLAST of Carg17796 vs. ExPASy TrEMBL
Match: A0A6J1H9P8 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita moschata OX=3662 GN=LOC111461380 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.5e-89
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy TrEMBL
Match: A0A6J1JHR3 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111485820 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 5.7e-89
Identity = 165/166 (99.40%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy TrEMBL
Match: A0A5D3E4B6 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00850 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 9.7e-89
Identity = 163/166 (98.19%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEP+FQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPIT+AGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy TrEMBL
Match: A0A1S3B858 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo OX=3656 GN=LOC103487227 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 9.7e-89
Identity = 163/166 (98.19%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEP+FQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPIT+AGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. ExPASy TrEMBL
Match: A0A0A0LXT5 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis sativus OX=3659 GN=Csa_1G657460 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.2e-88
Identity = 162/166 (97.59%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MALVTTAEVCDANPQLIV+GELRALEP+FQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGW+GIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWSGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HPMKAYKKRVGEKHVPIT+AGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg17796 vs. TAIR 10
Match: AT5G16450.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 295.4 bits (755), Expect = 2.8e-80
Identity = 140/166 (84.34%), Postives = 156/166 (93.98%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MA VTTAEVCDAN +LI +G+LRAL+P+FQIYGRRQ+FSGPVVT+KVFEDN LIR+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HP+KA KK +GE+ VP+ IAGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg17796 vs. TAIR 10
Match: AT5G16450.2 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 295.4 bits (755), Expect = 2.8e-80
Identity = 140/166 (84.34%), Postives = 156/166 (93.98%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MA VTTAEVCDAN +LI +G+LRAL+P+FQIYGRRQ+FSGPVVT+KVFEDN LIR+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HP+KA KK +GE+ VP+ IAGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg17796 vs. TAIR 10
Match: AT3G02770.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 290.0 bits (741), Expect = 1.2e-78
Identity = 139/166 (83.73%), Postives = 153/166 (92.17%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MA VTTAEVCDAN ++I +G+LRAL+PVFQIYGRRQ+FSGPVVT+KVFEDN LIR FLEE
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGI+VNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           HP+KA KK +GE+ V + IAGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg17796 vs. TAIR 10
Match: AT5G56260.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 248.8 bits (634), Expect = 3.0e-66
Identity = 117/166 (70.48%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 1   MALVTTAEVCDANPQLIVNGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60
           MA   TAE CD+N +LI NG+LRAL P+F+IYG+R+ FSGP+VTLKVFEDNVL+R  LE 
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120
           KG G VLV+DGGGS RCA++GGN    AQNNGW+GIVVNGCVRDVDEIN CD+GVRAL S
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 167
           +P+K+ KK  GEK+VP+ I GT I DGEWLYAD+DGIL+S+TELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

The following BLAST results are available for this feature:

BLAST of Carg17796 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960655.1	3.1e-89	100.00	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita moschata] >XP_0...	[more]
XP_022988625.1	1.2e-88	99.40	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita maxima] >XP_022...	[more]
XP_008443692.1	2.0e-88	98.19	PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo] ...	[more]
XP_023515582.1	2.6e-88	98.80	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita pepo subsp. pep...	[more]
XP_004139103.1	4.5e-88	97.59	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis sativus] >KGN6667...	[more]

BLAST of Carg17796 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFE0	3.9e-79	84.34	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana OX...	[more]
Q9M8R9	1.7e-77	83.73	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FH13	4.2e-65	70.48	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana OX...	[more]
Q46ZV6	8.6e-50	54.09	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensi...	[more]
A9AHQ6	7.2e-49	56.25	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Burkholderia multivorans ...	[more]

BLAST of Carg17796 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H9P8	1.5e-89	100.00	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JHR3	5.7e-89	99.40	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5D3E4B6	9.7e-89	98.19	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3B858	9.7e-89	98.19	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo OX=3656 GN=LOC1034872...	[more]
A0A0A0LXT5	2.2e-88	97.59	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis sativus OX=3659 GN=Csa_1G6...	[more]

BLAST of Carg17796 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16450.1	2.8e-80	84.34	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT5G16450.2	2.8e-80	84.34	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT3G02770.1	1.2e-78	83.73	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT5G56260.1	3.0e-66	70.48	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	PFAM	PF03737	RraA-like	coord: 6..159 e-value: 1.9E-42 score: 145.0
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	CDD	cd16841	RraA_family	coord: 7..160 e-value: 2.43857E-54 score: 166.866
None	No IPR available	GENE3D	3.50.30.40		coord: 3..166 e-value: 2.2E-48 score: 166.0
None	No IPR available	PANTHER	PTHR33254:SF20	4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE	coord: 4..166
None	No IPR available	PANTHER	PTHR33254	4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED	coord: 4..166
IPR010203	Regulator of ribonuclease activity A	TIGRFAM	TIGR01935	TIGR01935	coord: 6..160 e-value: 4.2E-65 score: 216.2
IPR036704	Ribonuclease E inhibitor RraA/RraA-like superfamily	SUPERFAMILY	89562	RraA-like	coord: 4..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17796-RA	Carg17796-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0051252	regulation of RNA metabolic process
molecular_function	GO:0047443	4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008948	oxaloacetate decarboxylase activity
molecular_function	GO:0008428	ribonuclease inhibitor activity