Carg17770 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg17770
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncalumenin-like
LocationCarg_Chr04: 17561405 .. 17566252 (+)
RNA-Seq ExpressionCarg17770
SyntenyCarg17770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTGTCTTCATCTCTGGATTTTTAGCTTTCTGCACCCAGCTGACAGCAAAAACCCAAAGCTACTTCGTTGGCTGTGTGAGGAAGAAATAAGGTAATGAAATTTTATGAAGTTATGTTCTTAACAGTTATCACAAATTTTCTCAATGCTTTATTTCCGTTAGTTTCAAAGCCTTTTATCTACTGCCTTTGCATTAGCATGCTCAGTTTGTCTTCATGTCTGCTAAATCTACCATTAGCGTTAAAAATGACTTTAGTCTATGGAGGTTCTTACTGAGAGCATTGAAATAAAGACGTTTGATTTTCTATAATTTCATAATATATCTCATTTTGGTTATTCTTGAACCTCTCATCGAACTTTGGAATTAATTAATTTCAAGACAAGAACCATTTAATGCTTGATTGAAGTGACTTGAATTGTGATAGATATATGATGACAGGTCCTTGCTTTTCTGTTTTGTGGAGTTTTGTTGGCTGAATTATTTTTGCCATATGTTTCTATCTATATATTAAATACTTTTTTTTTTTCCATATATGTGAGTTTCTGACAAGCTTGCATGTACTTTGACTAATCTAATGGGACAACCACTCGACTCTGCTACATTTGGTTGCCAGACACATAAGATACTCATAAGGGGATAAAAGTGCGAAAGAGGGTGATAAGGAATCCATCCATAAGTTAAGAAGGATTACAAAAACTTTCTCCAATTGCGTTTATAAAAAGAGTCATCATTACAAAATTACTTGGCTGTTGCACTTCGAAAGGAAGCTTTATAATGGATGGCTGCCCGAACCTTCTTTCGTCTCTTGCATTATGGAGAAAATTTTATGGTTTATAAGAGCTCTGAACCTGTTCTCTTGAAGAATCTTCACTTTCTTTTTCAAAAAGTGGTAAAAACGCAGTTGCCATGGAGATTGCCTGAAGGTAGAGGAGAAACTCCAATTGGATACCAAAACTATCCAAGAATGTTCCCAGGCTATGCAGCAATATAGCCTTTTGTTCTTGAATCAAAGACAACAACGACTAAGAGATAAGGCCCAATAATGATTTTTAGTCTTTGAATAATCCAAGGTGGATTTATCCCTCCCCTTAGTGACGTTTGCAAGAAAAATATTTACTCTTGGGAGTTTGAAGAGACTTTGTTCAAGTAAGGACTCTATATTCCTTAAACTCCTAGAATATGACTCACATGAGAAAATTCTGCTGCTCTCTAAAGTCCACATCTGTTTTTCACGGTGATTGTTTGTGATTTGCATGCGGGAAATCAGCTCATTTATCTCCTTATTACAGAAAAGCCTTCTGAAATCAGGATTCTAAGTATTAGAGATAATGTTCAGATGCTCTTTGGCTGCAGTGATTTACTTTAGCCACATGATCTAATCTTGGAAAGAGAAGTTTCAAATAATTCTTTGCTGCCAAAGCATGGTTCTCTAATTTTTATTTGCAGGTGGTTTTTCATTAAGTTGCATATTGCATTAAAGTTCCATGGACATCATTTGCTAGTGCACTTCTCTTTCTTTACGAACCAACTCAACTCATTGTTTGCTTTTGGGCATCTTGTTTAATAGGCTTGAGGGTGATCTTTCCATCGGACAAATTGTCTTACCTAAATCTGAAAGGAAATCATAATTTAAAGTCAAAATCTATCTGGGACTGAGCAGCTTCTTGGCTGGTTTTCTTGGTCCAGTACTTTGTTACTGGGGAAGATGAAGGCCATTGGGATTTAAGTACTTTTGTCTGCCAGACACGAGTCTTTTTCGCCATTCTCATGAATTTTAACTTCCACAAATTCCTGGCAATATATACATAGCATCATTCTTCTTAGTATGTTTTTATTTGAACTGTGAGGAGTTCTCCATCAATCCTTTTCCATTCTGAGTTCAGCTGGTAAAGATCTAAAATATTATCTTTTTTCTAATGTAAGCTTCTAGTTCACTTAGTGGCGTAATAAAACTGCAACATTTTGTTCCCATCTCTTCAAAGATTTGGAGAAAGATTTCTAATTTTTAGCCGTCCACCACATGAGGGAATATCTACCATCCTTTCTAGGATTTCATCTCTCCATTTATTGAATTTGATGAAAACTATCGCTCTATTCTCTGTATAGACTCAGCATTCTAGCTTCATCACTCTCACATATAAGCAGTGTTTTCTTGGTTGGAAAGGATTTAACACACATCTTATAATGTATCTGTGGATCTCTTAAGAACCTTTAGCCGTTTTTCATGGAAGCATCTACTCAAAACAACTGACATTGTTTCCAAATCTATTTGCAGTATCGCCTTTGAACTGATCTTAGTCACACCTGTTTCTTGTGTAGCTTGTCTTCCTCTTGCTCGTTGTTCTCTAATTAGTTCTTTAATTGCAAAGGATGTATACTTCCCCTTCCAGAGGCTTGGGAATGATTACTTCTCTTATCATCTCTCCATTGAAATCCTTCTGGAGGCTTGGGAATAATTACCTATCTTATCATCTTTTGATGATTATTTCTGATTGAAAGGCTTTCTTCATGCAGTTTTTTTTTTTTTTTAATTGCTTCCTTATATATAGCTTTTAGGCTGCATCCTGGCCCTTAGGATGTTTTCCTTTTCTTCTATCCGCATGTACCTTTCTTCATTTCTTATAAAAAGTTCCGTTCTGTCAACTCCAGTTCAGAGGGGATAGGTAGGTTATGTTTCTTTTCATTGTTTTATAGTTATGGACTGTTGGCAAGTCTCCTCAGCCTCTGCTGATTAAGGAGAAAATGGGCTTGGCTGGAAGAGGATGACAACATGAGAGAAAAGGAGATGAAGAATCAAATTGGGTCGATTGTTTTTTTGGTAGGCAGGTAGGTATTGTTTCCTTTCATTTGTTCCATAGTGATGAACTGTTGGCATCAGCCTCCTCAGCCTTTTCTGATTAAGGATTAAACGGGCCCAGCTAGAAGAGGATGGTAACATGAGAGAAAAGGAGATGAAGAAGCAGATTGTATCGATCATTTTCAGTTTCCCCCCCTAATTTATAAAAGAAAAAGAAAGGTGGACCGTGAGGAAACAGTTATTGTAGAATTCCATTATGGTTGAAGCTCAAAATCACAACAGTTGGGAGGCATAACCAAGGAGCTTACCTTGCTCTATATTAACGTCGACAAGAAAAATATTGCTTTGTTCGAGTATTTTTTATCTGAACCAACGTGGTAGCTCCTACTCTAGCAGTTCTCTCAAAATCTAATTGTAGGTCTTAATCTCACAATGAAAGAACCATTTTTAGTCCAATATTTTTAGTACATATATGCATATGGTAATGTTACATTTCACTATGTAAATTATCTATCTATCTATCTATCTATATATATATATATATATAGTATGTTAATAAATGTTATTTTCTTATAACACACGTTAGGCATTTTTGTTAGGATAAATAAATTCATTACCAATCTCAAATAAATTTTTGTTGTGGATAGTATCTGCATTGCCAAATCTATAAGCATTCCCTTTCTTGTACATTTTTGAGACTTTCATTCATTTAAGGAGCTTCTCAGCAGCTGTTTTTCGTGGCTTCAATGAAATGTATCTTATAAATAATCTATGAAGAAGAAACAATAGAGCTCACATACATGTTTTTTTTTTCAAGCTTAATTTATTTACTGTTTGCAAGCTTAATTTATTTGGAAGGGTGTAATCTTTTCTATGGTTTGGGGTTTGGCATTTCAGCACCTTTGCTAACTAGCCAATTTGTGTGTGGCCTTGTAGGGAGAGAGATTCAGACAAGGATGGAAAGATTAACTTCAACGAGTTTTTCCATGGACTCTTTGACATGGTGAGAAATTTTGATGAGAATCACAATTCTTCACATCATTTAGAAGATTCCAGGGATGGCCCCGCCAGAAACTTGTTCAAGGTGCTAGACAAAGATAATGACGGGTAATTAAATTTATGATGTGAACAGCTTCTTCATACATGGAAACTGCAGAGTTCCTTGGCAACTTTAATGATTTCTTTGTGTTATTTTTTCTCATAGATACCTATCTGATGAAGAGCTGCTACCCATAATCGGAAAAATCCACCCTACAGAGCATTACTATGCAAAACAACAAGCGGAATATATCCTACAGCAGGTACTCATGCATGACAAAATTTTGAAACCCAAATGAAATTTGAAGAATTTTTGGCCTTGTTGTTATGATATTGCTTGGATACTGCACACAATAATGGAATAAACAATCATCTTGTAATAATACGCCCTTGTCATGAGACTCTCATGTCTGGCTGCACAAGTATTTTCTTTAATTTTGACCAATCATCCACCCGTGCATGATGCATTAAGATTTATGTAAAATCATTTTTAGAACGATTAAAATCAGTCATTAAAACACAACCATTCACCAAAGATTTCCTGTTTGAACATTGTGTAATAATGCTGCAGCAAGAGATTTCAGTTTCCCCTTGATTCATTTTATCTTTTAATGTATTGTATAGCATAATTGTTTGTGTTATTTGTTCCTCATTGATTTTGATCTGATATACCAAGTTTTTCTCTCAGCCAAAGAACATTAATATATTGTATGACATGATTTTATCTTTGGCATTTGATATAGAAAAATTTTCTTGCAAATGTTTGTTATCTCAATTAGAAAACATTACGCCTAGATCGAAATAAGACTGGAAAACATGTTCTTCGTGCCGAGTAAGCGAGTATGTCTATTGATCAACAACTCCTTTGTATCTCTACTTTTCAGGCAGATGCAAATAAAGATGGACGTCTCACCTTGGCAGAAATGATTAATCATCCATATGTATTTTACAGCGCCATTTTCAACGAAGATGACGAGGATGACTATGATCTCCACGATGAGTTTCGTTAA

mRNA sequence

ATGATTTGTCTTCATCTCTGGATTTTTAGCTTTCTGCACCCAGCTGACAGCAAAAACCCAAAGCTACTTCGTTGGCTGTGTGAGGAAGAAATAAGGGAGAGAGATTCAGACAAGGATGGAAAGATTAACTTCAACGAGTTTTTCCATGGACTCTTTGACATGGTGAGAAATTTTGATGAGAATCACAATTCTTCACATCATTTAGAAGATTCCAGGGATGGCCCCGCCAGAAACTTGTTCAAGGTGCTAGACAAAGATAATGACGGATACCTATCTGATGAAGAGCTGCTACCCATAATCGGAAAAATCCACCCTACAGAGCATTACTATGCAAAACAACAAGCGGAATATATCCTACAGCAGGCAGATGCAAATAAAGATGGACGTCTCACCTTGGCAGAAATGATTAATCATCCATATGTATTTTACAGCGCCATTTTCAACGAAGATGACGAGGATGACTATGATCTCCACGATGAGTTTCGTTAA

Coding sequence (CDS)

ATGATTTGTCTTCATCTCTGGATTTTTAGCTTTCTGCACCCAGCTGACAGCAAAAACCCAAAGCTACTTCGTTGGCTGTGTGAGGAAGAAATAAGGGAGAGAGATTCAGACAAGGATGGAAAGATTAACTTCAACGAGTTTTTCCATGGACTCTTTGACATGGTGAGAAATTTTGATGAGAATCACAATTCTTCACATCATTTAGAAGATTCCAGGGATGGCCCCGCCAGAAACTTGTTCAAGGTGCTAGACAAAGATAATGACGGATACCTATCTGATGAAGAGCTGCTACCCATAATCGGAAAAATCCACCCTACAGAGCATTACTATGCAAAACAACAAGCGGAATATATCCTACAGCAGGCAGATGCAAATAAAGATGGACGTCTCACCTTGGCAGAAATGATTAATCATCCATATGTATTTTACAGCGCCATTTTCAACGAAGATGACGAGGATGACTATGATCTCCACGATGAGTTTCGTTAA

Protein sequence

MICLHLWIFSFLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRLTLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR
Homology
BLAST of Carg17770 vs. NCBI nr
Match: KAG7032674.1 (hypothetical protein SDJN02_06724, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 342.4 bits (877), Expect = 2.1e-90
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MICLHLWIFSFLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDE 60
           MICLHLWIFSFLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDE
Sbjct: 1   MICLHLWIFSFLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDE 60

Query: 61  NHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQ 120
           NHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQ
Sbjct: 61  NHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQ 120

Query: 121 QADANKDGRLTLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           QADANKDGRLTLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR
Sbjct: 121 QADANKDGRLTLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 162

BLAST of Carg17770 vs. NCBI nr
Match: XP_022990888.1 (calumenin-like [Cucurbita maxima])

HSP 1 Score: 318.5 bits (815), Expect = 3.2e-83
Identity = 151/152 (99.34%), Postives = 151/152 (99.34%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKLL WLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED
Sbjct: 228 FLHPADSKNPKLLHWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 287

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL
Sbjct: 288 SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 347

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR
Sbjct: 348 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 379

BLAST of Carg17770 vs. NCBI nr
Match: KAG6601976.1 (Calumenin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 317.8 bits (813), Expect = 5.5e-83
Identity = 151/152 (99.34%), Postives = 151/152 (99.34%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKL RWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED
Sbjct: 228 FLHPADSKNPKLPRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 287

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL
Sbjct: 288 SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 347

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR
Sbjct: 348 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 379

BLAST of Carg17770 vs. NCBI nr
Match: XP_022923022.1 (calumenin-like [Cucurbita moschata])

HSP 1 Score: 317.8 bits (813), Expect = 5.5e-83
Identity = 151/152 (99.34%), Postives = 151/152 (99.34%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKLLRWLC EEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED
Sbjct: 228 FLHPADSKNPKLLRWLCGEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 287

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL
Sbjct: 288 SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 347

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR
Sbjct: 348 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 379

BLAST of Carg17770 vs. NCBI nr
Match: XP_023536883.1 (calumenin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 313.5 bits (802), Expect = 1.0e-81
Identity = 148/152 (97.37%), Postives = 149/152 (98.03%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKLL WLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED
Sbjct: 228 FLHPADSKNPKLLHWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 287

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           +RDGPARNLF VLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL
Sbjct: 288 ARDGPARNLFNVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 347

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TL EMINHPYVFYSAIFNEDDEDDYDLHDEFR
Sbjct: 348 TLEEMINHPYVFYSAIFNEDDEDDYDLHDEFR 379

BLAST of Carg17770 vs. ExPASy Swiss-Prot
Match: Q91614 (Neuronal calcium sensor 1 OS=Xenopus laevis OX=8355 GN=ncs1 PE=2 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 2.5e-06
Identity = 35/109 (32.11%), Postives = 50/109 (45.87%), Query Frame = 0

Query: 35  DSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDE 94
           D +KDG+I F+EF   L    R              + D   R  FK+ D DNDGY++  
Sbjct: 73  DENKDGRIEFSEFIQALSVTSRG-------------TLDEKLRWAFKLYDLDNDGYITRN 132

Query: 95  ELLPIIGKIH---------PTEHYYAKQQAEYILQQADANKDGRLTLAE 135
           E+L I+  I+         P E    +++ + I    D N DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNSDGKLTLQE 168

BLAST of Carg17770 vs. ExPASy Swiss-Prot
Match: P62167 (Neuronal calcium sensor 1 OS=Gallus gallus OX=9031 GN=NCS1 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 3.3e-06
Identity = 35/109 (32.11%), Postives = 50/109 (45.87%), Query Frame = 0

Query: 35  DSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDE 94
           D +KDG+I F+EF   L    R              + D   R  FK+ D DNDGY++  
Sbjct: 73  DENKDGRIEFSEFIQALSVTSRG-------------TLDEKLRWAFKLYDLDNDGYITRN 132

Query: 95  ELLPIIGKIH---------PTEHYYAKQQAEYILQQADANKDGRLTLAE 135
           E+L I+  I+         P E    +++ + I    D N DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQE 168

BLAST of Carg17770 vs. ExPASy Swiss-Prot
Match: P62166 (Neuronal calcium sensor 1 OS=Homo sapiens OX=9606 GN=NCS1 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 3.3e-06
Identity = 35/109 (32.11%), Postives = 50/109 (45.87%), Query Frame = 0

Query: 35  DSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDE 94
           D +KDG+I F+EF   L    R              + D   R  FK+ D DNDGY++  
Sbjct: 73  DENKDGRIEFSEFIQALSVTSRG-------------TLDEKLRWAFKLYDLDNDGYITRN 132

Query: 95  ELLPIIGKIH---------PTEHYYAKQQAEYILQQADANKDGRLTLAE 135
           E+L I+  I+         P E    +++ + I    D N DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQE 168

BLAST of Carg17770 vs. ExPASy Swiss-Prot
Match: Q8BNY6 (Neuronal calcium sensor 1 OS=Mus musculus OX=10090 GN=Ncs1 PE=1 SV=3)

HSP 1 Score: 53.1 bits (126), Expect = 3.3e-06
Identity = 35/109 (32.11%), Postives = 50/109 (45.87%), Query Frame = 0

Query: 35  DSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDE 94
           D +KDG+I F+EF   L    R              + D   R  FK+ D DNDGY++  
Sbjct: 73  DENKDGRIEFSEFIQALSVTSRG-------------TLDEKLRWAFKLYDLDNDGYITRN 132

Query: 95  ELLPIIGKIH---------PTEHYYAKQQAEYILQQADANKDGRLTLAE 135
           E+L I+  I+         P E    +++ + I    D N DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQE 168

BLAST of Carg17770 vs. ExPASy Swiss-Prot
Match: Q5RC90 (Neuronal calcium sensor 1 OS=Pongo abelii OX=9601 GN=NCS1 PE=2 SV=3)

HSP 1 Score: 53.1 bits (126), Expect = 3.3e-06
Identity = 35/109 (32.11%), Postives = 50/109 (45.87%), Query Frame = 0

Query: 35  DSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLEDSRDGPARNLFKVLDKDNDGYLSDE 94
           D +KDG+I F+EF   L    R              + D   R  FK+ D DNDGY++  
Sbjct: 73  DENKDGRIGFSEFIQALSVTSRG-------------TLDEKLRWAFKLYDLDNDGYITRN 132

Query: 95  ELLPIIGKIH---------PTEHYYAKQQAEYILQQADANKDGRLTLAE 135
           E+L I+  I+         P E    +++ + I    D N DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQE 168

BLAST of Carg17770 vs. ExPASy TrEMBL
Match: A0A6J1JUL5 (calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111487642 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.6e-83
Identity = 151/152 (99.34%), Postives = 151/152 (99.34%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKLL WLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED
Sbjct: 228 FLHPADSKNPKLLHWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 287

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL
Sbjct: 288 SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 347

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR
Sbjct: 348 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 379

BLAST of Carg17770 vs. ExPASy TrEMBL
Match: A0A6J1E506 (calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111430825 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.7e-83
Identity = 151/152 (99.34%), Postives = 151/152 (99.34%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKLLRWLC EEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED
Sbjct: 228 FLHPADSKNPKLLRWLCGEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 287

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL
Sbjct: 288 SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 347

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR
Sbjct: 348 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 379

BLAST of Carg17770 vs. ExPASy TrEMBL
Match: A0A6J1FMS7 (calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 9.8e-78
Identity = 140/152 (92.11%), Postives = 147/152 (96.71%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKLL WLCEEEIRERDSDKDGKINFNEFFHGLFDMVRN+DENHNSSHH ED
Sbjct: 228 FLHPADSKNPKLLHWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSED 287

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLF VLDKDNDG+LSDEELLPIIGKIHP+EHYYA+QQAEYI+QQADA+KDGRL
Sbjct: 288 SRDGPARNLFAVLDKDNDGHLSDEELLPIIGKIHPSEHYYAEQQAEYIIQQADADKDGRL 347

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TL EMI+HPYVFYSAIFNEDDEDDYD HDEFR
Sbjct: 348 TLTEMIDHPYVFYSAIFNEDDEDDYDSHDEFR 379

BLAST of Carg17770 vs. ExPASy TrEMBL
Match: A0A5D3CVB0 (Calumenin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00460 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.3e-77
Identity = 139/152 (91.45%), Postives = 147/152 (96.71%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKL+ WLCEEEIRERDSDKDGKINFNEFFHGLFDMVRN+DENHNSSHH ED
Sbjct: 224 FLHPADSKNPKLIHWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSED 283

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLF VLDKDNDG+LS+EELLPIIGKIHP+EHYYAKQQAEYI+QQADA+KDGRL
Sbjct: 284 SRDGPARNLFAVLDKDNDGHLSNEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRL 343

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TL EMI+HPYVFYSAIFNEDDEDDYD HDEFR
Sbjct: 344 TLTEMIDHPYVFYSAIFNEDDEDDYDFHDEFR 375

BLAST of Carg17770 vs. ExPASy TrEMBL
Match: A0A1S3CNB9 (calumenin-B-like OS=Cucumis melo OX=3656 GN=LOC103502856 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.3e-77
Identity = 139/152 (91.45%), Postives = 147/152 (96.71%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLED 70
           FLHPADSKNPKL+ WLCEEEIRERDSDKDGKINFNEFFHGLFDMVRN+DENHNSSHH ED
Sbjct: 224 FLHPADSKNPKLIHWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSED 283

Query: 71  SRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRL 130
           SRDGPARNLF VLDKDNDG+LS+EELLPIIGKIHP+EHYYAKQQAEYI+QQADA+KDGRL
Sbjct: 284 SRDGPARNLFAVLDKDNDGHLSNEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRL 343

Query: 131 TLAEMINHPYVFYSAIFNEDDEDDYDLHDEFR 163
           TL EMI+HPYVFYSAIFNEDDEDDYD HDEFR
Sbjct: 344 TLTEMIDHPYVFYSAIFNEDDEDDYDFHDEFR 375

BLAST of Carg17770 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 244.6 bits (623), Expect = 5.5e-65
Identity = 117/154 (75.97%), Postives = 134/154 (87.01%), Query Frame = 0

Query: 11  FLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDE-NHNSSHHLE 70
           FLHPAD+KNPKLL WLC+EE+RERDSDKDGKI+F EFFHGLFD VRN++E NHNS+H   
Sbjct: 238 FLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYH 297

Query: 71  DSRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGR 130
           D  +GPA+ LF  LDK++DGYLSD ELLPII KIHPTEHYYAKQQA+YI+ QAD++KD R
Sbjct: 298 DLPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRR 357

Query: 131 LTLAEMINHPYVFYSAIFNEDD-EDDYDLHDEFR 163
           LTLAEMI HPYVFYSAIF+EDD +DDY  HDEFR
Sbjct: 358 LTLAEMIEHPYVFYSAIFDEDDTDDDYGFHDEFR 391

BLAST of Carg17770 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 109.0 bits (271), Expect = 3.6e-24
Identity = 57/152 (37.50%), Postives = 90/152 (59.21%), Query Frame = 0

Query: 10  SFLHPADSKNPKLLRWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSHHLE 69
           +FLHP DS+N    RW+ +E +   D++ DGK+ + EF    ++M + F +        E
Sbjct: 197 NFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKEFVKNAYEMYKEFAKFEKE----E 256

Query: 70  DSRDGPARNLFKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGR 129
           D      + LF  +D+D D +L  +EL PI+  + P E  YAK  + ++  +AD +KDG+
Sbjct: 257 DENVPTPQLLFAEMDRDKDRFLVADELRPILQYLQPGEMSYAKFYSTFLCHEADEDKDGK 316

Query: 130 LTLAEMINHPYVFYSAIFNED-DEDDYDLHDE 161
           L+L EM++H  VFY A+ +ED D++DY  HDE
Sbjct: 317 LSLEEMLHHEDVFYKAVHHEDLDDEDYFDHDE 344

BLAST of Carg17770 vs. TAIR 10
Match: AT2G41100.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 48.1 bits (113), Expect = 7.6e-06
Identity = 33/117 (28.21%), Postives = 58/117 (49.57%), Query Frame = 0

Query: 28  EEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSH--------HLEDSRDGPARNL 87
           ++ + E D D DG I+F EF   L+ M +N   +    H         L D +    R  
Sbjct: 139 QDMMNEVDLDGDGTIDFPEF---LYLMAKNQGHDQAPRHTKKTMVDYQLTDDQILEFREA 198

Query: 88  FKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRLTLAEMI 137
           F+V DK+ DGY++  EL   +  +  T+    K + + ++ +ADA+ DG ++ +E +
Sbjct: 199 FRVFDKNGDGYITVNELRTTMRSLGETQ---TKAELQDMINEADADGDGTISFSEFV 249

BLAST of Carg17770 vs. TAIR 10
Match: AT2G41100.2 (Calcium-binding EF hand family protein )

HSP 1 Score: 48.1 bits (113), Expect = 7.6e-06
Identity = 33/117 (28.21%), Postives = 58/117 (49.57%), Query Frame = 0

Query: 28  EEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSH--------HLEDSRDGPARNL 87
           ++ + E D D DG I+F EF   L+ M +N   +    H         L D +    R  
Sbjct: 14  QDMMNEVDLDGDGTIDFPEF---LYLMAKNQGHDQAPRHTKKTMVDYQLTDDQILEFREA 73

Query: 88  FKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRLTLAEMI 137
           F+V DK+ DGY++  EL   +  +  T+    K + + ++ +ADA+ DG ++ +E +
Sbjct: 74  FRVFDKNGDGYITVNELRTTMRSLGETQ---TKAELQDMINEADADGDGTISFSEFV 124

BLAST of Carg17770 vs. TAIR 10
Match: AT2G41100.3 (Calcium-binding EF hand family protein )

HSP 1 Score: 48.1 bits (113), Expect = 7.6e-06
Identity = 33/117 (28.21%), Postives = 58/117 (49.57%), Query Frame = 0

Query: 28  EEEIRERDSDKDGKINFNEFFHGLFDMVRNFDENHNSSH--------HLEDSRDGPARNL 87
           ++ + E D D DG I+F EF   L+ M +N   +    H         L D +    R  
Sbjct: 104 QDMMNEVDLDGDGTIDFPEF---LYLMAKNQGHDQAPRHTKKTMVDYQLTDDQILEFREA 163

Query: 88  FKVLDKDNDGYLSDEELLPIIGKIHPTEHYYAKQQAEYILQQADANKDGRLTLAEMI 137
           F+V DK+ DGY++  EL   +  +  T+    K + + ++ +ADA+ DG ++ +E +
Sbjct: 164 FRVFDKNGDGYITVNELRTTMRSLGETQ---TKAELQDMINEADADGDGTISFSEFV 214

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032674.12.1e-90100.00hypothetical protein SDJN02_06724, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022990888.13.2e-8399.34calumenin-like [Cucurbita maxima][more]
KAG6601976.15.5e-8399.34Calumenin, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022923022.15.5e-8399.34calumenin-like [Cucurbita moschata][more]
XP_023536883.11.0e-8197.37calumenin-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q916142.5e-0632.11Neuronal calcium sensor 1 OS=Xenopus laevis OX=8355 GN=ncs1 PE=2 SV=2[more]
P621673.3e-0632.11Neuronal calcium sensor 1 OS=Gallus gallus OX=9031 GN=NCS1 PE=1 SV=2[more]
P621663.3e-0632.11Neuronal calcium sensor 1 OS=Homo sapiens OX=9606 GN=NCS1 PE=1 SV=2[more]
Q8BNY63.3e-0632.11Neuronal calcium sensor 1 OS=Mus musculus OX=10090 GN=Ncs1 PE=1 SV=3[more]
Q5RC903.3e-0632.11Neuronal calcium sensor 1 OS=Pongo abelii OX=9601 GN=NCS1 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1JUL51.6e-8399.34calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111487642 PE=4 SV=1[more]
A0A6J1E5062.7e-8399.34calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111430825 PE=4 SV=1[more]
A0A6J1FMS79.8e-7892.11calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1[more]
A0A5D3CVB01.3e-7791.45Calumenin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G004... [more]
A0A1S3CNB91.3e-7791.45calumenin-B-like OS=Cucumis melo OX=3656 GN=LOC103502856 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08580.15.5e-6575.97Calcium-binding EF hand family protein [more]
AT4G27790.13.6e-2437.50Calcium-binding EF hand family protein [more]
AT2G41100.17.6e-0628.21Calcium-binding EF hand family protein [more]
AT2G41100.27.6e-0628.21Calcium-binding EF hand family protein [more]
AT2G41100.37.6e-0628.21Calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 75..103
e-value: 0.68
score: 19.0
coord: 26..54
e-value: 0.74
score: 18.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 78..136
e-value: 2.2E-10
score: 40.8
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 28..52
e-value: 0.0026
score: 17.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 71..106
score: 10.775421
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 27..57
score: 8.766953
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 110..145
score: 9.352757
NoneNo IPR availableGENE3D1.10.238.10coord: 72..138
e-value: 3.9E-12
score: 48.1
NoneNo IPR availableGENE3D1.10.238.10coord: 7..71
e-value: 1.5E-9
score: 39.9
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 10..162
NoneNo IPR availablePANTHERPTHR10827:SF83BNAC09G47400D PROTEINcoord: 10..162
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 123..135
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 84..96
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 35..47
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 9..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg17770-RACarg17770-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005509 calcium ion binding