Carg17769 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17769
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	calumenin-like
Location	Carg_Chr04: 17560115 .. 17561331 (+)
RNA-Seq Expression	Carg17769
Synteny	Carg17769

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAGATCTACGAAAGTCATCAATGATCAACAAAAACAAACTCCGTTTAGTCCAATTCTTCCACTCGTTTTCCTCTCGATTCCCCTTCGCTCTAAGAATCTCTCTGATCAATTTGTTCTCCAGAGATATCTCTCAAATTCTTAATCCCTCGGATTCCATCTTCTTCAACAATCGTCTCTTCAATCTCCGCATTCGCCATTGGCAGCCATTGTTGAACACTTCACAGAATGAACAGAGTTTCCATTATCATATACATTACAGTTGCCATTCTTCTTTTTCTTCTCATTTCCCACTCGCCCAAGAAAACCCCAGATCACCGCCATCGACGCCTCCATCTTCGCTCCAACTTCACTTTTGACCTTTCGCACAATCGCAGACACAACCATCACGAGGCGGTGCCGTTCGACCCTCTCGTCGCCGACATTGAGCGCCGTCGTGAAGACCGGCAATGGGAGAAGCAATATGTGGAAGAACACCATCCAGAGATGGCTGCGCATTTGACGGAATCGGCCCCCGGTGAAGAATCGCAGCCCGAGTGGGAGGATTTTGCGAATGCCGAGGATTATATCAATGACGATAATAGGTTCAATGTGACCGATCGGCTGACGTTGCTGTTTCCGAAGATTGATATTAATCCACATGATGATTTTGTCACTGTCGATGAGTTGACTGAGTGGAATTTGCAGCAGGCCGAGAAGGAAACTCTGCATAGGACTCGAAGGGAGTTGGAGACGCATGATAAGAATCATGATGGGCTTATTTCGTTTTCAGAGTACGAGCCCCCCAGTTGGATTCGCAATTCAGGTCTGTTTTTCTTGAAATGCTATTGGATTTTAGTTTCATGTATGCTCGACAATGAATTGGCGACTCTTTCTTCTTGTATTGTTCAATTCTTCCTCATTGCGAATTGCTATGCTGTGTAGAAAGATATGTTTGTACTGTTCAATCTCTAGGACTAAGAGTTTGCAAAACCCTATGATCGATTTCTTAAGCGATAAGTATTTTGACAACAACAAATGTAATTGCCCCACATATGATGCAGATTCATAGGAGGTTTTCCATTTGTTTTATGTTCCTTTGTGTATTACTCATTATTAAACTGTTCATCAGATAACAATTCCTTTGGGTATGATATGGGTTGGTGGAAACTTGAACATTTTAATGCATCGGATGCGGATGGAGATGGTCTTTTGAATTTGGCCGAGTTCAACGAGTAA

mRNA sequence

AAGAGATCTACGAAAGTCATCAATGATCAACAAAAACAAACTCCGTTTAGTCCAATTCTTCCACTCGTTTTCCTCTCGATTCCCCTTCGCTCTAAGAATCTCTCTGATCAATTTGTTCTCCAGAGATATCTCTCAAATTCTTAATCCCTCGGATTCCATCTTCTTCAACAATCGTCTCTTCAATCTCCGCATTCGCCATTGGCAGCCATTGTTGAACACTTCACAGAATGAACAGAGTTTCCATTATCATATACATTACAGTTGCCATTCTTCTTTTTCTTCTCATTTCCCACTCGCCCAAGAAAACCCCAGATCACCGCCATCGACGCCTCCATCTTCGCTCCAACTTCACTTTTGACCTTTCGCACAATCGCAGACACAACCATCACGAGGCGGTGCCGTTCGACCCTCTCGTCGCCGACATTGAGCGCCGTCGTGAAGACCGGCAATGGGAGAAGCAATATGTGGAAGAACACCATCCAGAGATGGCTGCGCATTTGACGGAATCGGCCCCCGGTGAAGAATCGCAGCCCGAGTGGGAGGATTTTGCGAATGCCGAGGATTATATCAATGACGATAATAGGTTCAATGTGACCGATCGGCTGACGTTGCTGTTTCCGAAGATTGATATTAATCCACATGATGATTTTGTCACTGTCGATGAGTTGACTGAGTGGAATTTGCAGCAGGCCGAGAAGGAAACTCTGCATAGGACTCGAAGGGAGTTGGAGACGCATGATAAGAATCATGATGGGCTTATTTCGTTTTCAGAGTACGAGCCCCCCAGTTGGATTCGCAATTCAGATAACAATTCCTTTGGGTATGATATGGGTTGGTGGAAACTTGAACATTTTAATGCATCGGATGCGGATGGAGATGGTCTTTTGAATTTGGCCGAGTTCAACGAGTAA

Coding sequence (CDS)

ATGAACAGAGTTTCCATTATCATATACATTACAGTTGCCATTCTTCTTTTTCTTCTCATTTCCCACTCGCCCAAGAAAACCCCAGATCACCGCCATCGACGCCTCCATCTTCGCTCCAACTTCACTTTTGACCTTTCGCACAATCGCAGACACAACCATCACGAGGCGGTGCCGTTCGACCCTCTCGTCGCCGACATTGAGCGCCGTCGTGAAGACCGGCAATGGGAGAAGCAATATGTGGAAGAACACCATCCAGAGATGGCTGCGCATTTGACGGAATCGGCCCCCGGTGAAGAATCGCAGCCCGAGTGGGAGGATTTTGCGAATGCCGAGGATTATATCAATGACGATAATAGGTTCAATGTGACCGATCGGCTGACGTTGCTGTTTCCGAAGATTGATATTAATCCACATGATGATTTTGTCACTGTCGATGAGTTGACTGAGTGGAATTTGCAGCAGGCCGAGAAGGAAACTCTGCATAGGACTCGAAGGGAGTTGGAGACGCATGATAAGAATCATGATGGGCTTATTTCGTTTTCAGAGTACGAGCCCCCCAGTTGGATTCGCAATTCAGATAACAATTCCTTTGGGTATGATATGGGTTGGTGGAAACTTGAACATTTTAATGCATCGGATGCGGATGGAGATGGTCTTTTGAATTTGGCCGAGTTCAACGAGTAA

Protein sequence

MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFDPLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRFNVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISFSEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE

Homology

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BLAST of Carg17769 vs. NCBI nr
Match: KAG7032673.1 (hypothetical protein SDJN02_06723 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 468.0 bits (1203), Expect = 4.6e-128
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD
Sbjct: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF
Sbjct: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE
Sbjct: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 227

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BLAST of Carg17769 vs. NCBI nr
Match: KAG6601976.1 (Calumenin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 465.7 bits (1197), Expect = 2.3e-127
Identity = 225/227 (99.12%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD
Sbjct: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAE+ETLHRTRRELETHDKNHDGLISF
Sbjct: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAERETLHRTRRELETHDKNHDGLISF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFN+
Sbjct: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFND 227

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BLAST of Carg17769 vs. NCBI nr
Match: XP_022923022.1 (calumenin-like [Cucurbita moschata])

HSP 1 Score: 465.7 bits (1197), Expect = 2.3e-127
Identity = 225/227 (99.12%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD
Sbjct: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAE+ETLHRTRRELETHDKNHDGLISF
Sbjct: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAERETLHRTRRELETHDKNHDGLISF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFN+
Sbjct: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFND 227

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BLAST of Carg17769 vs. NCBI nr
Match: XP_023536883.1 (calumenin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 463.0 bits (1190), Expect = 1.5e-126
Identity = 224/227 (98.68%), Postives = 226/227 (99.56%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFD SHNRRHNHHEAVPFD
Sbjct: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDPSHNRRHNHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAE+ETLHRTRRELETHDKNHDGLISF
Sbjct: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAERETLHRTRRELETHDKNHDGLISF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFN+
Sbjct: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFND 227

[Close]

BLAST of Carg17769 vs. NCBI nr
Match: XP_022990888.1 (calumenin-like [Cucurbita maxima])

HSP 1 Score: 458.0 bits (1177), Expect = 4.8e-125
Identity = 221/227 (97.36%), Postives = 225/227 (99.12%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFD SHNRRHNHHEAVPFD
Sbjct: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDPSHNRRHNHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVEEHHPEMAAHL ESAPGEESQPEWEDFANAEDYINDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLRESAPGEESQPEWEDFANAEDYINDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKIDI+PHDDFVTVDELTEWNLQQAE+ETLHRTRRELETHDKNHDGLISF
Sbjct: 121 NVTDRLTLLFPKIDIDPHDDFVTVDELTEWNLQQAERETLHRTRRELETHDKNHDGLISF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDNNSFGYDMGWWK+EHFNASDADGDGLLNLAEFN+
Sbjct: 181 SEYEPPSWIRNSDNNSFGYDMGWWKIEHFNASDADGDGLLNLAEFND 227

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BLAST of Carg17769 vs. ExPASy Swiss-Prot
Match: Q6IP82 (Calumenin OS=Xenopus laevis OX=8355 GN=calu PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.6e-04
Identity = 34/107 (31.78%), Postives = 54/107 (50.47%), Query Frame = 0


Query: 124 DRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISFSEY 183
           +RL ++  KID++ +D +VT  ELT W  +  +K       R+ +  D + DGL+S+ EY
Sbjct: 71  ERLGMIVGKIDLD-NDGYVTEGELTAWIKKAQKKYVYDNVERQWQEFDLSQDGLVSWDEY 130

Query: 184 EPPSWIRNSD----NNSFGYDMGWWKLE-HFNASDADGDGLLNLAEF 226
              ++    D    +NSF Y     + E  F  +D DGD +    EF
Sbjct: 131 RNVTYGTYLDDQDPDNSFNYKQMMIRDERRFKMADKDGDLVATKEEF 176

[Close]

BLAST of Carg17769 vs. ExPASy Swiss-Prot
Match: Q28BT4 (Calumenin OS=Xenopus tropicalis OX=8364 GN=calu PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 5.7e-04
Identity = 44/157 (28.03%), Postives = 73/157 (46.50%), Query Frame = 0


Query: 76  EKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF--NVTDRLTLLFPKI 135
           EK+    H P+++  + + A  +    + + F  AE+    D        +RL ++  KI
Sbjct: 23  EKKDRVHHDPQLSEKVHDDA--QNFDYDHDAFLGAEEAKTFDQLTPEESKERLGMIVSKI 82

Query: 136 DINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISFSEYEPPSWIRNSD 195
           D++ +D +VT  ELT W  +  +K       R+ +  D N DGL+S+ EY   ++    D
Sbjct: 83  DLD-NDGYVTEGELTAWIKKAQKKYVYDNVERQWQEFDLNQDGLVSWDEYRNVTYGTYLD 142

Query: 196 ----NNSFGYDMGWWKLE-HFNASDADGDGLLNLAEF 226
               +NSF Y     + E  F  +D DGD +    EF
Sbjct: 143 DPDPDNSFNYKQMMVRDERRFKMADQDGDLIATKEEF 176

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BLAST of Carg17769 vs. ExPASy TrEMBL
Match: A0A6J1E506 (calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111430825 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 225/227 (99.12%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD
Sbjct: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAE+ETLHRTRRELETHDKNHDGLISF
Sbjct: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAERETLHRTRRELETHDKNHDGLISF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFN+
Sbjct: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFND 227

[Close]

BLAST of Carg17769 vs. ExPASy TrEMBL
Match: A0A6J1JUL5 (calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111487642 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.3e-125
Identity = 221/227 (97.36%), Postives = 225/227 (99.12%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFD SHNRRHNHHEAVPFD
Sbjct: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDPSHNRRHNHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVEEHHPEMAAHL ESAPGEESQPEWEDFANAEDYINDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLRESAPGEESQPEWEDFANAEDYINDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKIDI+PHDDFVTVDELTEWNLQQAE+ETLHRTRRELETHDKNHDGLISF
Sbjct: 121 NVTDRLTLLFPKIDIDPHDDFVTVDELTEWNLQQAERETLHRTRRELETHDKNHDGLISF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDNNSFGYDMGWWK+EHFNASDADGDGLLNLAEFN+
Sbjct: 181 SEYEPPSWIRNSDNNSFGYDMGWWKIEHFNASDADGDGLLNLAEFND 227

[Close]

BLAST of Carg17769 vs. ExPASy TrEMBL
Match: A0A6J1FMS7 (calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.0e-109
Identity = 191/227 (84.14%), Postives = 213/227 (93.83%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           M++VSII+YITVA+LL LL+SHSPKK+P+ RHRRL LRSNFTF  SH+  H+HHEAVPFD
Sbjct: 1   MSKVSIIVYITVAVLLLLLVSHSPKKSPNRRHRRLKLRSNFTFAPSHHDDHHHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVE+HHPEMAAHLTE APGEESQPEWEDFA+AEDY+NDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEQHHPEMAAHLTEHAPGEESQPEWEDFADAEDYLNDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKID++P D FV VDELTEWNLQQA++ETLHRT+RELETHDKNHDGL+SF
Sbjct: 121 NVTDRLTLLFPKIDVHPSDGFVAVDELTEWNLQQAQRETLHRTQRELETHDKNHDGLVSF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSW RNSDN+SFG++MGWWK EHFNASDADGDGLLNL EFN+
Sbjct: 181 SEYEPPSWARNSDNSSFGFNMGWWKFEHFNASDADGDGLLNLTEFND 227

[Close]

BLAST of Carg17769 vs. ExPASy TrEMBL
Match: A0A6J1JYP4 (calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111489489 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.2e-107
Identity = 187/227 (82.38%), Postives = 211/227 (92.95%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           M++VSII+YITVA+LL LL+SHSPKK+P+ RHRRL LRSNFTF  SH+  H+HHEAVPFD
Sbjct: 1   MSKVSIIVYITVAVLLLLLVSHSPKKSPNRRHRRLKLRSNFTFAPSHHDDHHHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVE+HHPEMAAHLTE APGEESQPEWEDFA+AEDY+ND+NRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEQHHPEMAAHLTEHAPGEESQPEWEDFADAEDYLNDNNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRLTLLFPKID++P D FV VDEL EWNLQQ ++ETLHRT+RELETHDKNHDGL+SF
Sbjct: 121 NVTDRLTLLFPKIDVHPPDGFVAVDELIEWNLQQTQRETLHRTQRELETHDKNHDGLVSF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSW+RNSDN+SFG++MGWWK EHFN SDADGDGLLNL EFN+
Sbjct: 181 SEYEPPSWVRNSDNSSFGFNMGWWKFEHFNVSDADGDGLLNLTEFND 227

[Close]

BLAST of Carg17769 vs. ExPASy TrEMBL
Match: A0A6J1CJ22 (calumenin-like OS=Momordica charantia OX=3673 GN=LOC111011909 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.8e-107
Identity = 191/227 (84.14%), Postives = 209/227 (92.07%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           M++VSIIIYITVA+LL LL+SHSPKKTP+HRHRRL LRSNFTF  S    H H EA+PFD
Sbjct: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPS----HQHREAIPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLVADIERRREDRQWEKQYVE HHPE+AAHLTE APGEESQPEWEDFA+AEDY+NDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           NVTDRL+LLFPKID++P D FV VDELTEWNLQQAE+ETLHRT+RE+ETHDKNHDGL+SF
Sbjct: 121 NVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSF 180

Query: 181 SEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAEFNE 228
           SEYEPPSWIRNSDN+SFGYDMGWWKL HFNASDADGDG LNL EFN+
Sbjct: 181 SEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFND 223

[Close]

BLAST of Carg17769 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 305.4 bits (781), Expect = 3.7e-83
Identity = 148/243 (60.91%), Postives = 184/243 (75.72%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDH--------RHRRLHLRSNFTFDLSHNRRHN 60
           M++ S+I+YITV IL+  L+S+SPKK  DH        +H RL LRS+F F      +  
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNF------KPT 60

Query: 61  HHEAVPFDPLVADIERRREDRQWEKQYVEEHHPEMAAHLT--------ESAPGEESQPEW 120
            H+ VPFDPLVAD+ERRRED++WE+QY+E  HPE+ +H          E APG ESQPEW
Sbjct: 61  RHDPVPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEW 120

Query: 121 EDFANAEDYINDDNRFNVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTR 180
           E+F +AEDY+ND+ +FNVTDRL LLFPKID++P D F+T  ELTEW +Q + KE +HRT+
Sbjct: 121 EEFMDAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQ 180

Query: 181 RELETHDKNHDGLISFSEYEPPSWIRNSDNNSFGYDMGWWKLEHFNASDADGDGLLNLAE 228
           R+L+ HD+N DG ISFSEYEPPSW+R SDNNSFGYDMGWWK EHFNASDA+GDGLLNL E
Sbjct: 181 RDLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTE 237

[Close]

BLAST of Carg17769 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.0e-20
Identity = 71/227 (31.28%), Postives = 106/227 (46.70%), Query Frame = 0


Query: 1   MNRVSIIIYITVAILLFLLISHSPKKTPDHRHRRLHLRSNFTFDLSHNRRHNHHEAVPFD 60
           M +V +   +T  I+  +L++H  +            ++     L   R     E   FD
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQN-----------QTQSIEGLITRRIGRRLEMPVFD 60

Query: 61  PLVADIERRREDRQWEKQYVEEHHPEMAAHLTESAPGEESQPEWEDFANAEDYINDDNRF 120
           PLV  IER              H  E      E+A  E+           E+Y   + R 
Sbjct: 61  PLVTRIERL------------SHEKEAGTKTVEAAKEEKDD-------MFEEYFAQERRL 120

Query: 121 NVTDRLTLLFPKIDINPHDDFVTVDELTEWNLQQAEKETLHRTRRELETHDKNHDGLISF 180
           N T R+  LFP +D +P D FV++ EL  W +QQ E   ++RT +ELE  DK+ DG+I+F
Sbjct: 121 NTTMRIKFLFPLLDASPRDGFVSLKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITF 180

Query: 181 SEYEPPSWIRNSDNNSFGY-DMGWWKLEHFNASDADGDGLLNLAEFN 227
            EY P    ++ + N  G+ + GWW +E F  SD D +G L++ EFN
Sbjct: 181 EEYLPQFSKQDIEKNEKGHGEAGWW-MEQFKNSDFDHNGSLDIEEFN 196

The following BLAST results are available for this feature:

BLAST of Carg17769 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032673.1	4.6e-128	100.00	hypothetical protein SDJN02_06723 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6601976.1	2.3e-127	99.12	Calumenin, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022923022.1	2.3e-127	99.12	calumenin-like [Cucurbita moschata]	[more]
XP_023536883.1	1.5e-126	98.68	calumenin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022990888.1	4.8e-125	97.36	calumenin-like [Cucurbita maxima]	[more]

BLAST of Carg17769 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6IP82	2.6e-04	31.78	Calumenin OS=Xenopus laevis OX=8355 GN=calu PE=2 SV=1	[more]
Q28BT4	5.7e-04	28.03	Calumenin OS=Xenopus tropicalis OX=8364 GN=calu PE=2 SV=1	[more]

BLAST of Carg17769 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E506	1.1e-127	99.12	calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111430825 PE=4 SV=1	[more]
A0A6J1JUL5	2.3e-125	97.36	calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111487642 PE=4 SV=1	[more]
A0A6J1FMS7	3.0e-109	84.14	calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1	[more]
A0A6J1JYP4	2.2e-107	82.38	calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111489489 PE=4 SV=1	[more]
A0A6J1CJ22	4.8e-107	84.14	calumenin-like OS=Momordica charantia OX=3673 GN=LOC111011909 PE=4 SV=1	[more]

BLAST of Carg17769 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08580.1	3.7e-83	60.91	Calcium-binding EF hand family protein	[more]
AT4G27790.1	2.0e-20	31.28	Calcium-binding EF hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 207..226 e-value: 0.07 score: 12.7 coord: 171..183 e-value: 0.093 score: 12.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 209..227 score: 8.432208
None	No IPR available	GENE3D	1.10.238.10		coord: 121..227 e-value: 2.5E-7 score: 32.7
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 1..227
None	No IPR available	PANTHER	PTHR10827:SF83	BNAC09G47400D PROTEIN	coord: 1..227
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 213..225
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 171..183
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 124..225

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17769-RA	Carg17769-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding