Carg17545 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg17545
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionmitogen-activated protein kinase kinase kinase NPK1
LocationCarg_Chr12: 10280423 .. 10285537 (-)
RNA-Seq ExpressionCarg17545
SyntenyCarg17545
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTCTCTCTCTCTCTCTCTAGATTCTGATTTTCTTGCCGACCGCCATTAATCGGAAACTCTCCTTATTCCCTGTTCTTCGCAGATTCTTCATTCCCTCGATCTCTCCCGATCACTCAAATGCAAGATATCTTTGCTTCTGTTCGCCGATCATTGGTGTTTCGTGCTCCTGTTGAGAATGAGGATTCGCATTCTCCTGCAATTGGAGTTGGAGCCCTAGTTGATAAGATTAACTCCAGTATTAGGAAGTCCAGAGTGTTTTCTAGGCACTCGCCATCGTCGTCGGCTTATCCTCCGATTCTCAAGGAAACTGAGCCTCCGATTCGATGGCGCAAGGGCGAGTTGATTGGCTGCGGCGCCTTTGGTCGGGTGTATATGGGCATGAATCTTGGCTCTGGCGAGCTTCTCGCTGTTAAACAGGTTTGCTGGCTTTGGAGTTCGATTTTGGTTTTTTGGTTACCTCTTTGTAATGTTTACTCATCTGTTATTGGTGAATTGTGCTTTTGAAGCTTGCTTTTGAGTTTCCATTTCTCTTGATTGCAACCGATGCTTTGTTTGAGTTCTGATTCGATTTTGGATTTTCTTCGTTTTCTTTTGTTGTTTCACCCATTCGTACATCTTGTGAAATGATTACTGCATATTGTCGGGTGTAGGATCTCATTTTGACAATTTTCTTCGTTGTTCTCTTTCAGGTTCTGATTGCTGCAAATGGCGCTTCGAAGGAGAAAGCGCAGGTCTTCTTTTTAGTGTGCCTTTCGACAGCTGATTTTAATTTTATAATTTTCTAGTTTGGTTGAATTGGTTACTTGTTCTTTCATTACCCCAAACTCTAAGATTGTACTTATACTATAGTCTATATATAGAGATGCATGCATACATAGTTGGTTATCTTGGATCACAGATGACTGACTATATATGGCATTTGCCACTAACCACATTCCTGATGCAGTCGCTAAAAATTGTTTGAATTAATTAATTTGAGTTTTTCATTGATATGAGTTAGATACTTGAAAGTTATAATCTTCCTTTTTGTGTTGGTTAATATGATTTTTAAACAAGGCTTCATTGTTTTCATCTTTATTTTTTAATCTCGTGCTTCATAATATTAAGTTTTTATTGTTTTTACATATTATGGGCTCGCTGTTAAGCTCCACAAGTCTTTAGAAATTTGTTGGCTGCTTAGTGAATATTATATTAGTATGATTTATGAGGAATTACGAAAGTTTTCAAATAGAACTGAAAAATGAGAAACAGCAAGAAATTTTGAACAAGTTGATGCATATTTCATATCTAAATGACCTTTATTTGTCAATTTTGAGTAGTTTTTGTTGCAACCAGGCTCATGTTAAGGAGCTTGAGGAAGAAGTGAAACTTCTCAAGGATCTTTCTCATCCAAATATTGTTGTGAGTATGGCCTTCTCTTCCATTTATATAAAGAAGCCCATTACTTTTCCCACTGAATACATTGTGTTGTTTCTTAGAGATATTTGGGCACGGTTAGGGAGGATGATTCTGTAAATATATTATTGGAATTTGTCCCTGGTGGATCAATATCATCTCTTTTGGGGAAATTTGGAGCCTTCCCTGAAGCAGTAAGTTATTCTATATTCTACTTTGATCATTCTAGCGTCAGAGCATTGAATGTCTAATCCTTTATACGTACTGTATGTAGGTTATAAGAACATATACCAAACAGTTATTACTGGGACTGGAATACTTGCACAAGAATGGTATCATGCACAGGGACATTAAGGTCGGTGTTAGTTAGTTATGATCATTATTATTTGCAGACAATTTTCTCATTCCCCTGTGTCAGTTATAAATATGTCGTGTCTCTTGTATTCTATTGCAGGGGGCGAATATTCTTGTAGATAACAAGGGATGCATTAAGCTTGCTGATTTTGGTGCATCGAAACAGGTTGTCGAGCTGGTAAAGTCAACGATCCTTGACTTTTATTTGGTTTGAAAGCTATCTGCCTTCTGATCAAGATTTCATTGTTGTAGGCTACAATTTCAGGTGCAAAGTCTATGAAGGGTACTCCATATTGGATGGCTCCTGAAGTAATTCTGCAGACTGGGCATAGTTTGTAAGAACTGAATGAAGTCAAACTCTACTTAGAATCTACATTCTCTTCGGAATATTTGATATCAATCATTAACTTTTAAATGTTGATTTCAATTTTTTTTTGACAAAAAATTTCGAAGCAAATATATTTTTAGTCAATAAAGCAGGTTCGAGCTCAGTTATGACCGAATTTTGATAGTTGTTCTTTTAGAGAATCAAATATGTAGTAAACAAATAATTGGTCTTATGTAGCCTTGCCTTTGACTAAATATTAATTTGATGAGATGGATGATCGTAAGTATGTGATAATTATTCCTAGCTCTGCGGATATATGGAGTGTTGGATGCACTGTTATTGAGATGGCTACAGGAAAGCCTCCTTGGAGCCAACAGTATCAAGAGGTATCTTATTACTTGAAGATGTTTGAATGGTGGTGGATTAAATCATTCTTGTAAAGTGAACTAACAATACATTTCTTTTAGGTTGCTGCTCTTTTTCATATAGGCACAACAAAGTCTCATCCACCAATCCCTGAGCAGCTTTCAGTTGAAGCTAAAGATTTTCTGTTGAAATGTTTGCAGAAGTACGTTTCATTTCTTTGCATTTCTAACCCTGATCTTAAGAAAACTAATCATATGTTAACAATGGTCCAACTTTGAGATTCGTTTGAAATATTTTTCTTTAGCTCCTCTTTGACACTATTTCTTATGGGAAGTCAAATTGATGTGGGCCAAATTGCAGGGAACCCAACTTAAGACCAGCTGCATCTGAACTCCTGAAGGTGATTTTTTACTTGATTAATATTTTTGTTTTACTCGACAAGAACAAATTGTCAGATGCATAGACTGACAGAAATAGTGCCAAAGGAGCGATAGTTTTAACTATCATCTAACCGTCCGGCTTTGTTGTGTGCAGCATCCTTTTGTTACTGGGGAGGACCCAGAATCTCTACTTGCATCACGTGATGCATGCACGGTTAGAGGATTAAAGAATCAGAATAATTTATTCATGATTATTTGTCTTCCTGTTTGTAACGGTAAATTTGCCATCTTCAGGAGCCCTTGGATACCCATTCACTTTTATATACCTCAAACCTTGAAATGAGGTGAATTTAAAAATTCAGCGACTGGAAGAATTGTGCATTCACCTGCTTGCAATATTAAGGTTTTTCACATTTTTTTTTCTTGACAGCAAAACTCCAACGCATCCTGGATCAAGTGATATTTGTAATTTGGATAGTCTGAGCTGCTCAAAACTATACACCGCAAACAAACTAGAAAATGATACGTGGGGAACAAACAGTGATAATGAAATGTGTCAGGTTGATGACAATGATGATTTTATGTTAGATGAAGTAAAACTCAGGTCTTCTATGACATCTGGGAATTTCAAGGTGAGGAAAATGTTTGGATCCATTTTCAGTTCTCCGTCCAGAGATCTCTGGAAATTAACTTCACTTTTTTTTTCTTTCTTTTTTTTGGGTGGGGTGGGGGTGGTTTATATAGAGTTATAACCCAATCTGTGAGCCGTCGGATGACGAGGACTGCAAATTTGATCAAAGTTCAGTAGTAAAGCAAGGAAGCGATTTAGATGAGCAAACTCTAGCACCTGGCAGCTGTTCTGAAGTTTTTGATGATGACCAAAACTTCTCATTTCCTAGTGGACGATCACTTTCTGAAGATGAGGATGAGCTTACTGAATCAAAAATTAAAGCATTCTTGGATAAGAAGGTTTGGATTTTCAGTTTGAAATAGCAACTAGTTCGTCTTTCTAATTATCCTCTTCTCTGCAGTTTTATTAAATGAACCTGTTTGATCTAAATTGTTGTACATGATTTCAATGCCTCAGGCTCTTGAATTGAAGAAACTGCAGTCACCACTATATGAGGAGTTTTACAATAGTTTGAATGGATCCTGCTCTCCAGTTTTTGTAGAGAGTAAACAAGATGAAACTACTCCTAAATACTTGAAATTGCCCCCAAAAAGTCGGTCACCAAGTCGAAGTCCCGGTCATCTATCCCCAGCATTCGATGCCTTCGGCACTGGGACTCCAGGGAGTGATAGCAGCAGCAGGGGTAATGCAAATGATCAGAGGTCACAACTCAATGATTGGAAAGGACTTCTAGTTGATGGTCAGCCAGAAGCTGGTAGTCCAAGGTTTGAATTTTGGATGTTCTTTTAGCAGGCTTAGTATCATTAACATCTGTAGCATTCTTGATACCTTGGTGAGTAACATGTTTTCTCATCTAGCAGTAAACACTATTCTGAAATACAGAGGAAGTGGAAAGAAGAGCTTGATCAAGAGCTCGAGAGGAAACGAGGTAAATAACTATTCTTTATGCCTTAAATAAGAAGATAGCAATCATACGACCAAGGAGTCTGAAAAATATACGGTAGATATTTATATATAGTAGGTGGTTATATCTGATAAAGCTATATATAGTAAACTGAAACCATATATATACCCGTATACCCCTTAGGTAGTGATTGAAGATGTATTTTTTCTATTCTCTGTATTTTCTCTGTACGAAGTCATTAATACAAAAACTTCCTTACATTTTCTTTCTCCTATTTCTTTGTGTTCATCTAGATCGATTGTGTGCGTTGTTGTGCGGTCTTAACAATTATTACATATGACTATGCAACTCTCTTGTTTACAGAGATGATGCGTCAAGCTGGCACGGGAGGCAAAACATCATCTCCAAAGGATAAAGCCATCAGCAGGCCGAGAGAGCGAACACGATTTGCATCTCCATTCCGGTAGATATCTACGTTTCATTTCGTCTTTACACAGTATGTAATTCTACCATGGCAATATGCATGATGTGACGAACTGTTGACAGGGACGACGCGTCGGGTGCAGGGAGGGAAAACAGGAAAAATTCATCTCCCGAGGAAGTGAGGAAAGATTAGCATAGCTTCAAACTACTTCAGCTGAGTTTCTCTTCTCATTTGTCCTTCCCTCTGCAGTCCTTGTGGAGTGTATGAAGTGAAGAAGCCCCCTAGCTGGGTAAGAAGCATGATCATGATCAGATTCTCAATGAAAGGGGA

mRNA sequence

TCTCTCTCTCTCTCTCTCTCTAGATTCTGATTTTCTTGCCGACCGCCATTAATCGGAAACTCTCCTTATTCCCTGTTCTTCGCAGATTCTTCATTCCCTCGATCTCTCCCGATCACTCAAATGCAAGATATCTTTGCTTCTGTTCGCCGATCATTGGTGTTTCGTGCTCCTGTTGAGAATGAGGATTCGCATTCTCCTGCAATTGGAGTTGGAGCCCTAGTTGATAAGATTAACTCCAGTATTAGGAAGTCCAGAGTGTTTTCTAGGCACTCGCCATCGTCGTCGGCTTATCCTCCGATTCTCAAGGAAACTGAGCCTCCGATTCGATGGCGCAAGGGCGAGTTGATTGGCTGCGGCGCCTTTGGTCGGGTGTATATGGGCATGAATCTTGGCTCTGGCGAGCTTCTCGCTGTTAAACAGGTTCTGATTGCTGCAAATGGCGCTTCGAAGGAGAAAGCGCAGGCTCATGTTAAGGAGCTTGAGGAAGAAGTGAAACTTCTCAAGGATCTTTCTCATCCAAATATTGTTAGATATTTGGGCACGGTTAGGGAGGATGATTCTGTAAATATATTATTGGAATTTGTCCCTGGTGGATCAATATCATCTCTTTTGGGGAAATTTGGAGCCTTCCCTGAAGCAGTTATAAGAACATATACCAAACAGTTATTACTGGGACTGGAATACTTGCACAAGAATGGTATCATGCACAGGGACATTAAGGGGGCGAATATTCTTGTAGATAACAAGGGATGCATTAAGCTTGCTGATTTTGGTGCATCGAAACAGGTTGTCGAGCTGGCTACAATTTCAGGTGCAAAGTCTATGAAGGGTACTCCATATTGGATGGCTCCTGAAGTAATTCTGCAGACTGGGCATAGTTTCTCTGCGGATATATGGAGTGTTGGATGCACTGTTATTGAGATGGCTACAGGAAAGCCTCCTTGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTTCATATAGGCACAACAAAGTCTCATCCACCAATCCCTGAGCAGCTTTCAGTTGAAGCTAAAGATTTTCTGTTGAAATGTTTGCAGAAGGAACCCAACTTAAGACCAGCTGCATCTGAACTCCTGAAGCATCCTTTTGTTACTGGGGAGGACCCAGAATCTCTACTTGCATCACGTGATGCATGCACGGAGCCCTTGGATACCCATTCACTTTTATATACCTCAAACCTTGAAATGAGCAAAACTCCAACGCATCCTGGATCAAGTGATATTTGTAATTTGGATAGTCTGAGCTGCTCAAAACTATACACCGCAAACAAACTAGAAAATGATACGTGGGGAACAAACAGTGATAATGAAATGTGTCAGGTTGATGACAATGATGATTTTATGTTAGATGAAGTAAAACTCAGGTCTTCTATGACATCTGGGAATTTCAAGAGTTATAACCCAATCTGTGAGCCGTCGGATGACGAGGACTGCAAATTTGATCAAAGTTCAGTAGTAAAGCAAGGAAGCGATTTAGATGAGCAAACTCTAGCACCTGGCAGCTGTTCTGAAGTTTTTGATGATGACCAAAACTTCTCATTTCCTAGTGGACGATCACTTTCTGAAGATGAGGATGAGCTTACTGAATCAAAAATTAAAGCATTCTTGGATAAGAAGGCTCTTGAATTGAAGAAACTGCAGTCACCACTATATGAGGAGTTTTACAATAGTTTGAATGGATCCTGCTCTCCAGTTTTTGTAGAGAGTAAACAAGATGAAACTACTCCTAAATACTTGAAATTGCCCCCAAAAAGTCGGTCACCAAGTCGAAGTCCCGGTCATCTATCCCCAGCATTCGATGCCTTCGGCACTGGGACTCCAGGGAGTGATAGCAGCAGCAGGGGTAATGCAAATGATCAGAGGTCACAACTCAATGATTGGAAAGGACTTCTAGTTGATGGTCAGCCAGAAGCTGGTAGTCCAAGCAGTAAACACTATTCTGAAATACAGAGGAAGTGGAAAGAAGAGCTTGATCAAGAGCTCGAGAGGAAACGAGAGATGATGCGTCAAGCTGGCACGGGAGGCAAAACATCATCTCCAAAGGATAAAGCCATCAGCAGGCCGAGAGAGCGAACACGATTTGCATCTCCATTCCGGGACGACGCGTCGGGTGCAGGGAGGGAAAACAGGAAAAATTCATCTCCCGAGGAAGTGAGGAAAGATTAGCATAGCTTCAAACTACTTCAGCTGAGTTTCTCTTCTCATTTGTCCTTCCCTCTGCAGTCCTTGTGGAGTGTATGAAGTGAAGAAGCCCCCTAGCTGGGTAAGAAGCATGATCATGATCAGATTCTCAATGAAAGGGGA

Coding sequence (CDS)

ATGCAAGATATCTTTGCTTCTGTTCGCCGATCATTGGTGTTTCGTGCTCCTGTTGAGAATGAGGATTCGCATTCTCCTGCAATTGGAGTTGGAGCCCTAGTTGATAAGATTAACTCCAGTATTAGGAAGTCCAGAGTGTTTTCTAGGCACTCGCCATCGTCGTCGGCTTATCCTCCGATTCTCAAGGAAACTGAGCCTCCGATTCGATGGCGCAAGGGCGAGTTGATTGGCTGCGGCGCCTTTGGTCGGGTGTATATGGGCATGAATCTTGGCTCTGGCGAGCTTCTCGCTGTTAAACAGGTTCTGATTGCTGCAAATGGCGCTTCGAAGGAGAAAGCGCAGGCTCATGTTAAGGAGCTTGAGGAAGAAGTGAAACTTCTCAAGGATCTTTCTCATCCAAATATTGTTAGATATTTGGGCACGGTTAGGGAGGATGATTCTGTAAATATATTATTGGAATTTGTCCCTGGTGGATCAATATCATCTCTTTTGGGGAAATTTGGAGCCTTCCCTGAAGCAGTTATAAGAACATATACCAAACAGTTATTACTGGGACTGGAATACTTGCACAAGAATGGTATCATGCACAGGGACATTAAGGGGGCGAATATTCTTGTAGATAACAAGGGATGCATTAAGCTTGCTGATTTTGGTGCATCGAAACAGGTTGTCGAGCTGGCTACAATTTCAGGTGCAAAGTCTATGAAGGGTACTCCATATTGGATGGCTCCTGAAGTAATTCTGCAGACTGGGCATAGTTTCTCTGCGGATATATGGAGTGTTGGATGCACTGTTATTGAGATGGCTACAGGAAAGCCTCCTTGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTTCATATAGGCACAACAAAGTCTCATCCACCAATCCCTGAGCAGCTTTCAGTTGAAGCTAAAGATTTTCTGTTGAAATGTTTGCAGAAGGAACCCAACTTAAGACCAGCTGCATCTGAACTCCTGAAGCATCCTTTTGTTACTGGGGAGGACCCAGAATCTCTACTTGCATCACGTGATGCATGCACGGAGCCCTTGGATACCCATTCACTTTTATATACCTCAAACCTTGAAATGAGCAAAACTCCAACGCATCCTGGATCAAGTGATATTTGTAATTTGGATAGTCTGAGCTGCTCAAAACTATACACCGCAAACAAACTAGAAAATGATACGTGGGGAACAAACAGTGATAATGAAATGTGTCAGGTTGATGACAATGATGATTTTATGTTAGATGAAGTAAAACTCAGGTCTTCTATGACATCTGGGAATTTCAAGAGTTATAACCCAATCTGTGAGCCGTCGGATGACGAGGACTGCAAATTTGATCAAAGTTCAGTAGTAAAGCAAGGAAGCGATTTAGATGAGCAAACTCTAGCACCTGGCAGCTGTTCTGAAGTTTTTGATGATGACCAAAACTTCTCATTTCCTAGTGGACGATCACTTTCTGAAGATGAGGATGAGCTTACTGAATCAAAAATTAAAGCATTCTTGGATAAGAAGGCTCTTGAATTGAAGAAACTGCAGTCACCACTATATGAGGAGTTTTACAATAGTTTGAATGGATCCTGCTCTCCAGTTTTTGTAGAGAGTAAACAAGATGAAACTACTCCTAAATACTTGAAATTGCCCCCAAAAAGTCGGTCACCAAGTCGAAGTCCCGGTCATCTATCCCCAGCATTCGATGCCTTCGGCACTGGGACTCCAGGGAGTGATAGCAGCAGCAGGGGTAATGCAAATGATCAGAGGTCACAACTCAATGATTGGAAAGGACTTCTAGTTGATGGTCAGCCAGAAGCTGGTAGTCCAAGCAGTAAACACTATTCTGAAATACAGAGGAAGTGGAAAGAAGAGCTTGATCAAGAGCTCGAGAGGAAACGAGAGATGATGCGTCAAGCTGGCACGGGAGGCAAAACATCATCTCCAAAGGATAAAGCCATCAGCAGGCCGAGAGAGCGAACACGATTTGCATCTCCATTCCGGGACGACGCGTCGGGTGCAGGGAGGGAAAACAGGAAAAATTCATCTCCCGAGGAAGTGAGGAAAGATTAG

Protein sequence

MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPILKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKELEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTSNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEVKLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQDETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERTRFASPFRDDASGAGRENRKNSSPEEVRKD
Homology
BLAST of Carg17545 vs. NCBI nr
Match: KAG7021107.1 (Mitogen-activated protein kinase kinase kinase NPK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1362.4 bits (3525), Expect = 0.0e+00
Identity = 689/689 (100.00%), Postives = 689/689 (100.00%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420

Query: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480
           KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF
Sbjct: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480

Query: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540
           SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD
Sbjct: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540

Query: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600
           ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV
Sbjct: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600

Query: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660
           DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT
Sbjct: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660

Query: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 690
           RFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 689

BLAST of Carg17545 vs. NCBI nr
Match: XP_022937587.1 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1360.9 bits (3521), Expect = 0.0e+00
Identity = 688/689 (99.85%), Postives = 688/689 (99.85%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLL SRDACTEPLDTHSLLYTS
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLVSRDACTEPLDTHSLLYTS 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420

Query: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480
           KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF
Sbjct: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480

Query: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540
           SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD
Sbjct: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540

Query: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600
           ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV
Sbjct: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600

Query: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660
           DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT
Sbjct: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660

Query: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 690
           RFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 689

BLAST of Carg17545 vs. NCBI nr
Match: XP_022937588.1 (mitogen-activated protein kinase kinase kinase 2-like isoform X2 [Cucurbita moschata] >KAG6586264.1 Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1354.7 bits (3505), Expect = 0.0e+00
Identity = 687/689 (99.71%), Postives = 687/689 (99.71%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLL SRDACTEPLDTHSLLYTS
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLVSRDACTEPLDTHSLLYTS 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420

Query: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480
           KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF
Sbjct: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480

Query: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540
           SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD
Sbjct: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540

Query: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600
           ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV
Sbjct: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600

Query: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660
           DGQPEAGSP SKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT
Sbjct: 601 DGQPEAGSP-SKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660

Query: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 690
           RFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 688

BLAST of Carg17545 vs. NCBI nr
Match: XP_023536943.1 (mitogen-activated protein kinase kinase kinase NPK1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 680/691 (98.41%), Postives = 683/691 (98.84%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAY PI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYHPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTV EDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVSEDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLL SRDACTEPLDTHSLLYT 
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLVSRDACTEPLDTHSLLYTP 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT-NSDNEMCQVDDNDDFMLDE 420
           +LEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT NSDNEMCQ+DDNDDFMLDE
Sbjct: 361 DLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNNSDNEMCQIDDNDDFMLDE 420

Query: 421 VKLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQN 480
           VKLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQN
Sbjct: 421 VKLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQN 480

Query: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFY-NSLNGSCSPVFVESK 540
           FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFY NSLN SCSPVFVESK
Sbjct: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNNSLNASCSPVFVESK 540

Query: 541 QDETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGL 600
           QDETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGL
Sbjct: 541 QDETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGL 600

Query: 601 LVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRE 660
           LVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAI RPRE
Sbjct: 601 LVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAIGRPRE 660

Query: 661 RTRFASPFRDDASGAGRENRKNSSPEEVRKD 690
           +TRFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 KTRFASPFRDDASGAGRENRKNSSPEEVRKD 691

BLAST of Carg17545 vs. NCBI nr
Match: XP_022965613.1 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 677/690 (98.12%), Postives = 679/690 (98.41%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVA LFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAVLFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLR AASELLKHPFVTGEDPESLL SRDACTEPL+THSLLYT 
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRLAASELLKHPFVTGEDPESLLVSRDACTEPLETHSLLYTP 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT-NSDNEMCQVDDNDDFMLDE 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT NSDNEMCQ+D NDDFMLDE
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNNSDNEMCQIDGNDDFMLDE 420

Query: 421 VKLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQN 480
           VKLRSSM SGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQ LAPGS S VFDDDQN
Sbjct: 421 VKLRSSMISGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQILAPGSYSGVFDDDQN 480

Query: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ 540
           FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ
Sbjct: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ 540

Query: 541 DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL 600
           DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL
Sbjct: 541 DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL 600

Query: 601 VDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRER 660
           VDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAI RPRER
Sbjct: 601 VDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAIGRPRER 660

Query: 661 TRFASPFRDDASGAGRENRKNSSPEEVRKD 690
           TRFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 TRFASPFRDDASGAGRENRKNSSPEEVRKD 690

BLAST of Carg17545 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 1.3e-205
Identity = 422/701 (60.20%), Postives = 513/701 (73.18%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGA----LVDKINSSIRKSR--VFSR-HSPS 60
           MQD   SVRRSLVF+   ++ D  + A GVG+     V+K+ SSIRKS   +FS+ H P+
Sbjct: 1   MQDFIGSVRRSLVFK---QSGDFDTGAAGVGSGFGGFVEKLGSSIRKSSIGIFSKAHVPA 60

Query: 61  ----SSAYPP--ILKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANG 120
               S A  P    K+  PPIRWRKGE+IGCGAFGRVYMGMN+ SGELLA+K+V IA NG
Sbjct: 61  LPSISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNG 120

Query: 121 ASKEKAQAHVKELEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKF 180
           AS+E+AQAHV+ELEEEV LLK+LSHPNIVRYLGT RE  S+NILLEFVPGGSISSLLGKF
Sbjct: 121 ASRERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGSLNILLEFVPGGSISSLLGKF 180

Query: 181 GAFPEAVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELA 240
           G+FPE+VIR YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASK+VVELA
Sbjct: 181 GSFPESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELA 240

Query: 241 TISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFH 300
           T++GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCT+IEMATGKPPWSQQYQEVAALFH
Sbjct: 241 TMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFH 300

Query: 301 IGTTKSHPPIPEQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPES---LLASR 360
           IGTTKSHPPIPE LS E+KDFLLKCLQKEP+LR +AS LL+HPFVT E  E+   L +S 
Sbjct: 301 IGTTKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSF 360

Query: 361 DACTEPLDTHSLLYTSNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDT-WGT-NS 420
               E +    +   +++      +  G  D+C + ++ CS +Y  N L  ++ W   NS
Sbjct: 361 MGNPENMAAQRMDVRTSIIPDMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLGNS 420

Query: 421 DNEMCQVDDNDDFMLD-EVKLRSSMTS-GNFKSYNPICEPSDDEDCKFDQS-SVVKQGSD 480
           D++MCQ+ DNDDFM    VK  S + S  N+KS+NP+CEP +D  CKFD+S  + K  ++
Sbjct: 421 DDDMCQM-DNDDFMFGASVKCSSDLHSPANYKSFNPMCEPDNDWPCKFDESPELTKSQAN 480

Query: 481 L--DEQTLAPGSCSEV-FDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQS 540
           L  D+ T+ P +   + + +D  F+FPSG+S +ED+DELTESKI+AFLD+KA++LKKLQ+
Sbjct: 481 LHYDQATIKPTNNPIMSYKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKLQT 540

Query: 541 PLYEEFYNSLNGSCSPVFVESKQDETTPKYLKLPPKSRSPSRS-PGHLSPAFDAFGTGTP 600
           PLYE FYNSLN S +P  V +   E  P  + LPPKSRSP R     LS A +     +P
Sbjct: 541 PLYEGFYNSLNVSSTPSPVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAPSP 600

Query: 601 GSDS---SSRGNAND---QRSQL---NDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELD 660
            + S   S+ G  N    Q +QL   N+WK LL   Q EA + S   +SE QR+WKEELD
Sbjct: 601 VTHSKRISNIGGLNGEAIQEAQLPRHNEWKDLL-GSQREAVNSS---FSERQRRWKEELD 660

Query: 661 QELERKREMMRQAGTGGKTSSPKDKAISRPRERTRFASPFR 668
           +EL+RKRE+MRQA      S PKD  ++R R ++RFASP R
Sbjct: 661 EELQRKREIMRQA---VNLSPPKDPILNRCRSKSRFASPGR 690

BLAST of Carg17545 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 682.9 bits (1761), Expect = 3.6e-195
Identity = 408/691 (59.04%), Postives = 488/691 (70.62%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQD F SVRRSLVFR   ++++  +     G L DKI S IRKS++F +  PS S  PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSKIFIK--PSFSPPPPA 60

Query: 61  -LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKE 120
              +  PPI WRKG+LIG GAFG VYMGMNL SGELLAVKQVLIAAN ASKEK QAH++E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYT 180
           LEEEVKLLK+LSHPNIVRYLGTVREDD++NILLEFVPGGSISSLL KFG FPE+V+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASKQV ELAT++GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPE 300
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IGTTKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 QLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYT 360
            LS +AKDFLLKCLQ+ PNLRP ASELLKHPFV G+  ES      +    L T   L  
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 SNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDN-----EMCQVDDNDD 420
           +N + +   T     D+CN  SL+ S +     ++N      +DN     +MC +DD ++
Sbjct: 361 NNTKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGDEDDMCLIDD-EN 420

Query: 421 FMLDEVKLRSSMTSGNF--KSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSE 480
           F+  + ++ S++       KS + I + S     KFD+S     G+   E T++      
Sbjct: 421 FLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDES----PGNGEKESTMS------ 480

Query: 481 VFDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSP 540
             + DQ        S SED+DELTESKIKAFLD+KA +LKKLQ+PLYEEFYNSL  + SP
Sbjct: 481 -MECDQ-------PSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLI-TFSP 540

Query: 541 VFVE-----SKQDETTPKYLKLPPKSRSPSRSP--GHLSPAFDAFG-TGTPGSDSSSRGN 600
             +E     SK+++T   +LKLPPKSRSPSR P  G  S A DA   + +PGS  S   N
Sbjct: 541 SCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRELN 600

Query: 601 AN---DQRSQ------LNDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKR-E 660
            N   D+ SQ      + DW+GL+VD + E     +   SEI++KWKEELDQELERKR E
Sbjct: 601 INNGGDEASQDGVSARVTDWRGLVVDTKQELSQCVA--LSEIEKKWKEELDQELERKRQE 660

Query: 661 MMRQAGTGGKTSSPKDKAISRPRERTRFASP 666
           +MRQAG G   SSP+D+ +SR RE++RFASP
Sbjct: 661 IMRQAGLG---SSPRDRGMSRQREKSRFASP 664

BLAST of Carg17545 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 3.1e-194
Identity = 404/693 (58.30%), Postives = 487/693 (70.27%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPV--ENEDSHSPAIGVGALVDKINSSIRKSRVFSR-HSPSSSAY 60
           MQD+F SVRRSLVFR+    EN+++H P      L DKI S IRKS VF++  SP +++ 
Sbjct: 1   MQDLFGSVRRSLVFRSTTDDENQENHPPPF-PSLLADKITSCIRKSMVFAKSQSPPNNST 60

Query: 61  PPILKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHV 120
             I    +PPIRWRKG+LIG GAFG VYMGMNL SGELLAVKQVLI +N ASKEK QAH+
Sbjct: 61  VQI----KPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHI 120

Query: 121 KELEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRT 180
           +ELEEEVKLLK+LSHPNIVRYLGTVRED+++NILLEFVPGGSISSLL KFGAFPE+V+RT
Sbjct: 121 QELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRT 180

Query: 181 YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKG 240
           YT QLLLGLEYLH + IMHRDIKGANILVDN+GCIKLADFGASKQV ELATISGAKSMKG
Sbjct: 181 YTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKG 240

Query: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPI 300
           TPYWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIGTTKSHPPI
Sbjct: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPI 300

Query: 301 PEQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPES----LLASRDACTEPLDT 360
           P+ +S +A DFLLKCLQ+EPNLRP ASELLKHPFVTG+  ES    L +  D    PL +
Sbjct: 301 PDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPS 360

Query: 361 HSLLYTSNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDT--------WGTNSDNE 420
                 +N+   +T T     DICNL SL+C+  +    ++N++        +  + DN+
Sbjct: 361 E----LTNITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDND 420

Query: 421 MCQVDDNDDFMLDEVKLRSSMTSGN--FKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQ 480
           MC +DD ++F+    +   S+ +     KS + + E SD   CKFD++S    G+   E 
Sbjct: 421 MCLIDD-ENFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENS----GNGETET 480

Query: 481 TLAPGSCSEVFDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFY 540
            ++                P   S SEDE+ELTESKIKAFLD KA ELKKLQ+PLYEEFY
Sbjct: 481 KVS-----------MEVDHP---SYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFY 540

Query: 541 NSLNGSCSPVFVES-----KQDETTPKYLKLPPKSRSPS-----RSPGHLSPAFDAFGTG 600
           N +  +CSP+ +ES     K++E    +LKLPPKSRSPS     RSP   + A  A  + 
Sbjct: 541 NGMI-TCSPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDA--ACCSK 600

Query: 601 TPGSDSSSRGNANDQRSQLNDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELDQELERK- 660
           +P S +SS    N   S           G  +  +  S   SEI+RKWKEELDQELERK 
Sbjct: 601 SPESGNSSGAPKNSNASA----------GAEQESNSQSVALSEIERKWKEELDQELERKR 649

Query: 661 REMMRQAGTGGKTSSPKDKAISRPRERTRFASP 666
           RE+ RQAG G   SSP+D+++SR RE++RFASP
Sbjct: 661 REITRQAGMG---SSPRDRSLSRHREKSRFASP 649

BLAST of Carg17545 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 4.9e-184
Identity = 369/656 (56.25%), Postives = 458/656 (69.82%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRV--FSRHSPSSSAYP 60
           MQDI  SVRRSLVFR+ +  +D  S   G+   V KINSSIR SR+  FS+  P     P
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGG-GLSGFVGKINSSIRSSRIGLFSKPPP---GLP 60

Query: 61  PILKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVK 120
              KE  P IRWRKGELIGCGAFGRVYMGMNL SGELLA+KQVLIA + ASKEK Q H++
Sbjct: 61  APRKEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIR 120

Query: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTY 180
           ELEEEV+LLK+LSHPNIVRYLGTVRE DS+NIL+EFVPGGSISSLL KFG+FPE VI  Y
Sbjct: 121 ELEEEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMY 180

Query: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGT 240
           TKQLLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASK+VVELAT++GAKSMKGT
Sbjct: 181 TKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGT 240

Query: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300
           PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIP
Sbjct: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIP 300

Query: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTE---PLDTHS 360
           E LS EAKDFL+KCL KEP+LR +A+ELL+HPFVTG+  E   A R++ TE   P+ T  
Sbjct: 301 EDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQG 360

Query: 361 LLYTSNL-EMSKTPTHPGSSDICNLDSLSCSKLYTANKLEND-TWGTNSDNEMCQVDDND 420
           +   S++  + +  T  G  D+C L SL  S +Y      +   W     +++CQ D +D
Sbjct: 361 MNVRSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDD 420

Query: 421 DFMLDEVK--LRSSMTSGNFKSYNPICEPSDDEDCKFDQS-SVVKQGSDLDEQTLAPGSC 480
              ++ V+  + S  T  N KS+NP+C+ +D+  CKFD+S  V+K  S+L     +    
Sbjct: 421 LCNIESVRNNVLSQSTDLN-KSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQT 480

Query: 481 SEVFDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSC 540
               D++ + +F  G S++ED+ + TE KIK+FLD+KA +LK+LQ+PL EEF+N++N   
Sbjct: 481 GVPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMN--- 540

Query: 541 SPVFVESKQDETTPKYL-KLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQR 600
            P   +    +T    L  LP  S++P R P   S    A     P    SS+   N  R
Sbjct: 541 -PGIPQGALGDTNIYNLPNLPSISKTPKRLP---SRRLSAISDAMPSPLKSSKRTLNTSR 600

Query: 601 SQLNDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKT 646
              +  +   V+   + G  +S+ +SEI+RKW+EEL +ELER RE +R AG GGKT
Sbjct: 601 VMQSGTEPTQVNESTKKGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAGGKT 644

BLAST of Carg17545 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 1.5e-79
Identity = 150/309 (48.54%), Postives = 208/309 (67.31%), Query Frame = 0

Query: 68  IRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKELEEEVKLL 127
           I+W+KG+++G G +G VY+G+N  +GEL AVKQ+ I  +  S  K +  +    +E++++
Sbjct: 168 IKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEI-VDINSDPKLKNMILSFSKEIEVM 227

Query: 128 KDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTKQLLLGLE 187
           + L H NIVRYLGT  +   +++ LE++PGGSISSLLGKFGAF E VI+ YTKQ+L GL 
Sbjct: 228 RSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLS 287

Query: 188 YLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPYWMAPEVI 247
           +LH N I+HRDIKGANIL+D KG +KL+DFG SK    +  +S  KSM+GTPYWMAPEVI
Sbjct: 288 FLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGI--VSQFKSMQGTPYWMAPEVI 347

Query: 248 LQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQLSVEAKD 307
            QTGH  S+DIWS+GC ++EMAT +PPWS   +  A ++HI ++ S P IP  +S EA D
Sbjct: 348 KQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFD 407

Query: 308 FLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTSNLEMSKT 367
           FL  C +++P  RP A++LLKHPF+   D    L +      P  T S   T+    + T
Sbjct: 408 FLNLCFKRDPKERPDANQLLKHPFIMNLDDNIQLPT----ISPTTTLSTNTTNTTATTTT 467

Query: 368 PTHPGSSDI 377
             +  +S+I
Sbjct: 468 TNNATNSNI 469

BLAST of Carg17545 vs. ExPASy TrEMBL
Match: A0A6J1FBM5 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443951 PE=4 SV=1)

HSP 1 Score: 1360.9 bits (3521), Expect = 0.0e+00
Identity = 688/689 (99.85%), Postives = 688/689 (99.85%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLL SRDACTEPLDTHSLLYTS
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLVSRDACTEPLDTHSLLYTS 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420

Query: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480
           KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF
Sbjct: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480

Query: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540
           SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD
Sbjct: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540

Query: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600
           ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV
Sbjct: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600

Query: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660
           DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT
Sbjct: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660

Query: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 690
           RFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 689

BLAST of Carg17545 vs. ExPASy TrEMBL
Match: A0A6J1FGE5 (mitogen-activated protein kinase kinase kinase 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443951 PE=4 SV=1)

HSP 1 Score: 1354.7 bits (3505), Expect = 0.0e+00
Identity = 687/689 (99.71%), Postives = 687/689 (99.71%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLL SRDACTEPLDTHSLLYTS
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLVSRDACTEPLDTHSLLYTS 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420

Query: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480
           KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF
Sbjct: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480

Query: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540
           SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD
Sbjct: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540

Query: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600
           ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV
Sbjct: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600

Query: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660
           DGQPEAGSP SKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT
Sbjct: 601 DGQPEAGSP-SKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660

Query: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 690
           RFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 RFASPFRDDASGAGRENRKNSSPEEVRKD 688

BLAST of Carg17545 vs. ExPASy TrEMBL
Match: A0A6J1HPH3 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465457 PE=4 SV=1)

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 677/690 (98.12%), Postives = 679/690 (98.41%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVA LFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAVLFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLR AASELLKHPFVTGEDPESLL SRDACTEPL+THSLLYT 
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRLAASELLKHPFVTGEDPESLLVSRDACTEPLETHSLLYTP 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT-NSDNEMCQVDDNDDFMLDE 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT NSDNEMCQ+D NDDFMLDE
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNNSDNEMCQIDGNDDFMLDE 420

Query: 421 VKLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQN 480
           VKLRSSM SGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQ LAPGS S VFDDDQN
Sbjct: 421 VKLRSSMISGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQILAPGSYSGVFDDDQN 480

Query: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ 540
           FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ
Sbjct: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ 540

Query: 541 DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL 600
           DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL
Sbjct: 541 DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL 600

Query: 601 VDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRER 660
           VDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAI RPRER
Sbjct: 601 VDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAIGRPRER 660

Query: 661 TRFASPFRDDASGAGRENRKNSSPEEVRKD 690
           TRFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 TRFASPFRDDASGAGRENRKNSSPEEVRKD 690

BLAST of Carg17545 vs. ExPASy TrEMBL
Match: A0A6J1HRH2 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465457 PE=4 SV=1)

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 676/690 (97.97%), Postives = 678/690 (98.26%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVA LFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAVLFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLR AASELLKHPFVTGEDPESLL SRDACTEPL+THSLLYT 
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRLAASELLKHPFVTGEDPESLLVSRDACTEPLETHSLLYTP 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT-NSDNEMCQVDDNDDFMLDE 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGT NSDNEMCQ+D NDDFMLDE
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNNSDNEMCQIDGNDDFMLDE 420

Query: 421 VKLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQN 480
           VKLRSSM SGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQ LAPGS S VFDDDQN
Sbjct: 421 VKLRSSMISGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQILAPGSYSGVFDDDQN 480

Query: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ 540
           FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ
Sbjct: 481 FSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQ 540

Query: 541 DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL 600
           DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL
Sbjct: 541 DETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLL 600

Query: 601 VDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRER 660
           VDGQPEAGSP SKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAI RPRER
Sbjct: 601 VDGQPEAGSP-SKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAIGRPRER 660

Query: 661 TRFASPFRDDASGAGRENRKNSSPEEVRKD 690
           TRFASPFRDDASGAGRENRKNSSPEEVRKD
Sbjct: 661 TRFASPFRDDASGAGRENRKNSSPEEVRKD 689

BLAST of Carg17545 vs. ExPASy TrEMBL
Match: A0A6J1FBM2 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111443951 PE=4 SV=1)

HSP 1 Score: 1321.2 bits (3418), Expect = 0.0e+00
Identity = 666/667 (99.85%), Postives = 666/667 (99.85%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI
Sbjct: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60

Query: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120
           LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL
Sbjct: 61  LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKEL 120

Query: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180
           EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK
Sbjct: 121 EEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTK 180

Query: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240
           QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY
Sbjct: 181 QLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPY 240

Query: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300
           WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ
Sbjct: 241 WMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQ 300

Query: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYTS 360
           LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLL SRDACTEPLDTHSLLYTS
Sbjct: 301 LSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLVSRDACTEPLDTHSLLYTS 360

Query: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420
           NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV
Sbjct: 361 NLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDNEMCQVDDNDDFMLDEV 420

Query: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480
           KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF
Sbjct: 421 KLRSSMTSGNFKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSEVFDDDQNF 480

Query: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540
           SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD
Sbjct: 481 SFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSPVFVESKQD 540

Query: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600
           ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV
Sbjct: 541 ETTPKYLKLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQRSQLNDWKGLLV 600

Query: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660
           DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT
Sbjct: 601 DGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKTSSPKDKAISRPRERT 660

Query: 661 RFASPFR 668
           RFASPFR
Sbjct: 661 RFASPFR 667

BLAST of Carg17545 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 682.9 bits (1761), Expect = 2.6e-196
Identity = 408/691 (59.04%), Postives = 488/691 (70.62%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRVFSRHSPSSSAYPPI 60
           MQD F SVRRSLVFR   ++++  +     G L DKI S IRKS++F +  PS S  PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSKIFIK--PSFSPPPPA 60

Query: 61  -LKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKE 120
              +  PPI WRKG+LIG GAFG VYMGMNL SGELLAVKQVLIAAN ASKEK QAH++E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYT 180
           LEEEVKLLK+LSHPNIVRYLGTVREDD++NILLEFVPGGSISSLL KFG FPE+V+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASKQV ELAT++GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPE 300
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IGTTKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 QLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTEPLDTHSLLYT 360
            LS +AKDFLLKCLQ+ PNLRP ASELLKHPFV G+  ES      +    L T   L  
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 SNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDTWGTNSDN-----EMCQVDDNDD 420
           +N + +   T     D+CN  SL+ S +     ++N      +DN     +MC +DD ++
Sbjct: 361 NNTKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGDEDDMCLIDD-EN 420

Query: 421 FMLDEVKLRSSMTSGNF--KSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPGSCSE 480
           F+  + ++ S++       KS + I + S     KFD+S     G+   E T++      
Sbjct: 421 FLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDES----PGNGEKESTMS------ 480

Query: 481 VFDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSCSP 540
             + DQ        S SED+DELTESKIKAFLD+KA +LKKLQ+PLYEEFYNSL  + SP
Sbjct: 481 -MECDQ-------PSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLI-TFSP 540

Query: 541 VFVE-----SKQDETTPKYLKLPPKSRSPSRSP--GHLSPAFDAFG-TGTPGSDSSSRGN 600
             +E     SK+++T   +LKLPPKSRSPSR P  G  S A DA   + +PGS  S   N
Sbjct: 541 SCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRELN 600

Query: 601 AN---DQRSQ------LNDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKR-E 660
            N   D+ SQ      + DW+GL+VD + E     +   SEI++KWKEELDQELERKR E
Sbjct: 601 INNGGDEASQDGVSARVTDWRGLVVDTKQELSQCVA--LSEIEKKWKEELDQELERKRQE 660

Query: 661 MMRQAGTGGKTSSPKDKAISRPRERTRFASP 666
           +MRQAG G   SSP+D+ +SR RE++RFASP
Sbjct: 661 IMRQAGLG---SSPRDRGMSRQREKSRFASP 664

BLAST of Carg17545 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 646.0 bits (1665), Expect = 3.5e-185
Identity = 369/656 (56.25%), Postives = 458/656 (69.82%), Query Frame = 0

Query: 1   MQDIFASVRRSLVFRAPVENEDSHSPAIGVGALVDKINSSIRKSRV--FSRHSPSSSAYP 60
           MQDI  SVRRSLVFR+ +  +D  S   G+   V KINSSIR SR+  FS+  P     P
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGG-GLSGFVGKINSSIRSSRIGLFSKPPP---GLP 60

Query: 61  PILKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVK 120
              KE  P IRWRKGELIGCGAFGRVYMGMNL SGELLA+KQVLIA + ASKEK Q H++
Sbjct: 61  APRKEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIR 120

Query: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTY 180
           ELEEEV+LLK+LSHPNIVRYLGTVRE DS+NIL+EFVPGGSISSLL KFG+FPE VI  Y
Sbjct: 121 ELEEEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMY 180

Query: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGT 240
           TKQLLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASK+VVELAT++GAKSMKGT
Sbjct: 181 TKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGT 240

Query: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300
           PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIP
Sbjct: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIP 300

Query: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPESLLASRDACTE---PLDTHS 360
           E LS EAKDFL+KCL KEP+LR +A+ELL+HPFVTG+  E   A R++ TE   P+ T  
Sbjct: 301 EDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQG 360

Query: 361 LLYTSNL-EMSKTPTHPGSSDICNLDSLSCSKLYTANKLEND-TWGTNSDNEMCQVDDND 420
           +   S++  + +  T  G  D+C L SL  S +Y      +   W     +++CQ D +D
Sbjct: 361 MNVRSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDD 420

Query: 421 DFMLDEVK--LRSSMTSGNFKSYNPICEPSDDEDCKFDQS-SVVKQGSDLDEQTLAPGSC 480
              ++ V+  + S  T  N KS+NP+C+ +D+  CKFD+S  V+K  S+L     +    
Sbjct: 421 LCNIESVRNNVLSQSTDLN-KSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQT 480

Query: 481 SEVFDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNGSC 540
               D++ + +F  G S++ED+ + TE KIK+FLD+KA +LK+LQ+PL EEF+N++N   
Sbjct: 481 GVPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMN--- 540

Query: 541 SPVFVESKQDETTPKYL-KLPPKSRSPSRSPGHLSPAFDAFGTGTPGSDSSSRGNANDQR 600
            P   +    +T    L  LP  S++P R P   S    A     P    SS+   N  R
Sbjct: 541 -PGIPQGALGDTNIYNLPNLPSISKTPKRLP---SRRLSAISDAMPSPLKSSKRTLNTSR 600

Query: 601 SQLNDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELDQELERKREMMRQAGTGGKT 646
              +  +   V+   + G  +S+ +SEI+RKW+EEL +ELER RE +R AG GGKT
Sbjct: 601 VMQSGTEPTQVNESTKKGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAGGKT 644

BLAST of Carg17545 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 641.0 bits (1652), Expect = 1.1e-183
Identity = 373/628 (59.39%), Postives = 447/628 (71.18%), Query Frame = 0

Query: 63  ETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVKELEE 122
           + +PPIRWRKG+LIG GAFG VYMGMNL SGELLAVKQVLI +N ASKEK QAH++ELEE
Sbjct: 16  QIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEE 75

Query: 123 EVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGGSISSLLGKFGAFPEAVIRTYTKQL 182
           EVKLLK+LSHPNIVRYLGTVRED+++NILLEFVPGGSISSLL KFGAFPE+V+RTYT QL
Sbjct: 76  EVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQL 135

Query: 183 LLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGTPYWM 242
           LLGLEYLH + IMHRDIKGANILVDN+GCIKLADFGASKQV ELATISGAKSMKGTPYWM
Sbjct: 136 LLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWM 195

Query: 243 APEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEQLS 302
           APEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIGTTKSHPPIP+ +S
Sbjct: 196 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNIS 255

Query: 303 VEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDPES----LLASRDACTEPLDTHSLLY 362
            +A DFLLKCLQ+EPNLRP ASELLKHPFVTG+  ES    L +  D    PL +     
Sbjct: 256 SDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPSE---- 315

Query: 363 TSNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLENDT--------WGTNSDNEMCQVD 422
            +N+   +T T     DICNL SL+C+  +    ++N++        +  + DN+MC +D
Sbjct: 316 LTNITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDNDMCLID 375

Query: 423 DNDDFMLDEVKLRSSMTSGN--FKSYNPICEPSDDEDCKFDQSSVVKQGSDLDEQTLAPG 482
           D ++F+    +   S+ +     KS + + E SD   CKFD++S    G+   E  ++  
Sbjct: 376 D-ENFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENS----GNGETETKVS-- 435

Query: 483 SCSEVFDDDQNFSFPSGRSLSEDEDELTESKIKAFLDKKALELKKLQSPLYEEFYNSLNG 542
                         P   S SEDE+ELTESKIKAFLD KA ELKKLQ+PLYEEFYN +  
Sbjct: 436 ---------MEVDHP---SYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGMI- 495

Query: 543 SCSPVFVES-----KQDETTPKYLKLPPKSRSPS-----RSPGHLSPAFDAFGTGTPGSD 602
           +CSP+ +ES     K++E    +LKLPPKSRSPS     RSP   + A  A  + +P S 
Sbjct: 496 TCSPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDA--ACCSKSPESG 555

Query: 603 SSSRGNANDQRSQLNDWKGLLVDGQPEAGSPSSKHYSEIQRKWKEELDQELERK-REMMR 662
           +SS    N   S           G  +  +  S   SEI+RKWKEELDQELERK RE+ R
Sbjct: 556 NSSGAPKNSNASA----------GAEQESNSQSVALSEIERKWKEELDQELERKRREITR 604

Query: 663 QAGTGGKTSSPKDKAISRPRERTRFASP 666
           QAG G   SSP+D+++SR RE++RFASP
Sbjct: 616 QAGMG---SSPRDRSLSRHREKSRFASP 604

BLAST of Carg17545 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 280.8 bits (717), Expect = 3.0e-75
Identity = 150/359 (41.78%), Postives = 219/359 (61.00%), Query Frame = 0

Query: 39  SSIRKSRVFSRHSPSSSAYPPILKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAV 98
           S++  SR+   +  S S +            W+KG+ +G G FG+VY+G N   G++ A+
Sbjct: 193 SAVHGSRIGGGYETSPSGFS----------TWKKGKFLGSGTFGQVYLGFNSEKGKMCAI 252

Query: 99  KQVLIAANGASKEKAQAHVKELEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGG 158
           K+V + ++  + ++    +K+L +E+ LL  L HPNIV+Y G+   ++++++ LE+V GG
Sbjct: 253 KEVKVISDDQTSKEC---LKQLNQEINLLNQLCHPNIVQYYGSELSEETLSVYLEYVSGG 312

Query: 159 SISSLLGKFGAFPEAVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFG 218
           SI  LL  +G+F E VI+ YT+Q+L GL YLH    +HRDIKGANILVD  G IKLADFG
Sbjct: 313 SIHKLLKDYGSFTEPVIQNYTRQILAGLAYLHGRNTVHRDIKGANILVDPNGEIKLADFG 372

Query: 219 ASKQVVELATISGAKSMKGTPYWMAPEVIL-QTGHSFSADIWSVGCTVIEMATGKPPWSQ 278
            +K V   +T+    S KG+PYWMAPEV++ Q G++ + DIWS+GCT++EMAT KPPWS 
Sbjct: 373 MAKHVTAFSTM---LSFKGSPYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWS- 432

Query: 279 QYQEVAALFHIGTTKSHPPIPEQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDP 338
           Q++ VAA+F IG +K  P IP+ LS +AK+F+  CLQ+ P +RP AS+LL+HPF+     
Sbjct: 433 QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRN--- 492

Query: 339 ESLLASRDACTE-PLDTHSLLYTSNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLEN 396
                +R A T  P D     Y  N  +     +PG     N      S+   +   EN
Sbjct: 493 ----TTRVASTSLPKDFPPRSYDGNFSLPTREPYPGRLSHDNYAKQPLSRTIKSPSREN 527

BLAST of Carg17545 vs. TAIR 10
Match: AT1G53570.3 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 280.8 bits (717), Expect = 3.0e-75
Identity = 150/359 (41.78%), Postives = 219/359 (61.00%), Query Frame = 0

Query: 39  SSIRKSRVFSRHSPSSSAYPPILKETEPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAV 98
           S++  SR+   +  S S +            W+KG+ +G G FG+VY+G N   G++ A+
Sbjct: 193 SAVHGSRIGGGYETSPSGFS----------TWKKGKFLGSGTFGQVYLGFNSEKGKMCAI 252

Query: 99  KQVLIAANGASKEKAQAHVKELEEEVKLLKDLSHPNIVRYLGTVREDDSVNILLEFVPGG 158
           K+V + ++  + ++    +K+L +E+ LL  L HPNIV+Y G+   ++++++ LE+V GG
Sbjct: 253 KEVKVISDDQTSKEC---LKQLNQEINLLNQLCHPNIVQYYGSELSEETLSVYLEYVSGG 312

Query: 159 SISSLLGKFGAFPEAVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFG 218
           SI  LL  +G+F E VI+ YT+Q+L GL YLH    +HRDIKGANILVD  G IKLADFG
Sbjct: 313 SIHKLLKDYGSFTEPVIQNYTRQILAGLAYLHGRNTVHRDIKGANILVDPNGEIKLADFG 372

Query: 219 ASKQVVELATISGAKSMKGTPYWMAPEVIL-QTGHSFSADIWSVGCTVIEMATGKPPWSQ 278
            +K V   +T+    S KG+PYWMAPEV++ Q G++ + DIWS+GCT++EMAT KPPWS 
Sbjct: 373 MAKHVTAFSTM---LSFKGSPYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWS- 432

Query: 279 QYQEVAALFHIGTTKSHPPIPEQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEDP 338
           Q++ VAA+F IG +K  P IP+ LS +AK+F+  CLQ+ P +RP AS+LL+HPF+     
Sbjct: 433 QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRN--- 492

Query: 339 ESLLASRDACTE-PLDTHSLLYTSNLEMSKTPTHPGSSDICNLDSLSCSKLYTANKLEN 396
                +R A T  P D     Y  N  +     +PG     N      S+   +   EN
Sbjct: 493 ----TTRVASTSLPKDFPPRSYDGNFSLPTREPYPGRLSHDNYAKQPLSRTIKSPSREN 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021107.10.0e+00100.00Mitogen-activated protein kinase kinase kinase NPK1 [Cucurbita argyrosperma subs... [more]
XP_022937587.10.0e+0099.85mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 [Cucurbita m... [more]
XP_022937588.10.0e+0099.71mitogen-activated protein kinase kinase kinase 2-like isoform X2 [Cucurbita mosc... [more]
XP_023536943.10.0e+0098.41mitogen-activated protein kinase kinase kinase NPK1-like [Cucurbita pepo subsp. ... [more]
XP_022965613.10.0e+0098.12mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 [Cucurbita m... [more]
Match NameE-valueIdentityDescription
Q405411.3e-20560.20Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220403.6e-19559.04Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Q9FZ363.1e-19458.30Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
O220424.9e-18456.25Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Q54R821.5e-7948.54Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1FBM50.0e+0099.85mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 OS=Cucurbita... [more]
A0A6J1FGE50.0e+0099.71mitogen-activated protein kinase kinase kinase 2-like isoform X2 OS=Cucurbita mo... [more]
A0A6J1HPH30.0e+0098.12mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 OS=Cucurbita... [more]
A0A6J1HRH20.0e+0097.97mitogen-activated protein kinase kinase kinase NPK1-like isoform X2 OS=Cucurbita... [more]
A0A6J1FBM20.0e+0099.85mitogen-activated protein kinase kinase kinase NPK1-like isoform X3 OS=Cucurbita... [more]
Match NameE-valueIdentityDescription
AT1G09000.12.6e-19659.04NPK1-related protein kinase 1 [more]
AT3G06030.13.5e-18556.25NPK1-related protein kinase 3 [more]
AT1G54960.11.1e-18359.39NPK1-related protein kinase 2 [more]
AT1G53570.23.0e-7541.78mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.33.0e-7541.78mitogen-activated protein kinase kinase kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 618..638
NoneNo IPR availableCOILSCoilCoilcoord: 103..130
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 69..362
e-value: 2.1E-85
score: 288.3
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 76..328
e-value: 1.3E-17
score: 61.6
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 35..336
e-value: 5.9E-27
score: 92.2
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 35..336
e-value: 5.9E-27
score: 92.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 615..638
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 573..593
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 538..689
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 650..689
NoneNo IPR availablePANTHERPTHR48011:SF63ACTIVATED PROTEIN KINASE KINASE KINASE 3, MAPKKK3, MEKK3, PUTATIVE-RELATEDcoord: 46..568
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 46..568
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 69..332
e-value: 3.83913E-167
score: 477.398
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 70..332
e-value: 1.9E-100
score: 349.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 71..332
e-value: 1.7E-71
score: 240.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 70..332
score: 51.777802
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 76..99
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 194..206
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 69..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg17545-RACarg17545-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity