Carg17447 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg17447
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncalcium uptake protein, mitochondrial-like
LocationCarg_Chr12: 10958260 .. 10962599 (-)
RNA-Seq ExpressionCarg17447
SyntenyCarg17447
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGAATTATAGTATGTTTTCTTTGACGTCTTTAGCTTCAAAATCTAATTCCAAATCGATCCATAAAGTTATAGCTTCACCACAACTTATCACTGTTCTATTGTCTTCTCAACGAGCTGGCTCCGATCGAGATAATAGAAGAAGCTCCATATTCGACTTCTTAACCAAATCAATTGCTTCGGGAGCTTTGATTGTCGGAGTTGGGTCGTTTTTTAGCTCAAATTTGCAAGTTTCTTTTGCTGACTTTCCGAAACAAACGACATGGACGGTGAACGAAGATCGGATTCCATACCCATTGCCGAATCAAAATGCAACGAAGAAACCAAAGTTTTTATTTGGAGGTAAGTGAATGTTCATCGACCATAGTATGATCTTGTAAGATTCCACGTTGATTGGGGAGGAGAACGAAATATCCTTTACAAGGGTGTGGAAACCTCTCTCGACACGTTTTAAAAATCTTGAAGGGAAGCCCGAAGGGTTGCCCAAAGAGGACAATTATAAATGGTATTAGAGCTGGGCAATGTGTCAGTAAGGAAGCTGAGCCCCGAAGAGAGTGGACTCGAGGTGATGTGCCAATAAGGACCCTGGGCTCCAAAGGGGGTGAATTGTGGGTGTCCACATGATTGGAGAATGGAAAACCCTGGGGACCCTGAGCCCCGAAGGGGTGGATTGTAAGATCCCACCTTAGTTAGGGAGGAGAATGAATTAAAAATCTTTCGTTCATGTTTTGTATCCAAATGGTTGTAGATGATTATAGACAGATGTTTTTCTTTGTATCCAGCATTTTAACCTAGTGGGTCATGGTAAATTTAATCTTTTGTTCATGTTTTGTGTCCGATCGATTCTAGATAATTACAAAAGATGGGGTGTTTTTCGTATCCAACATTTTAACCTAATGAATCACAATCATCATGGTAAATTTAATCTTTTGTTCATGTTTTGTGTCCGATCGATTCTAGATAATTACAAAAGATCGGGTGTTTTTCGTATCCAACATTTTAACCTAATGGGTCACGGTAAATTTAATCTTTTGTTCATATTTTGCGTCCGATCATTTCCAGATGATTACAGAAGACGAGTGTTTTTCAACTACGAAAAACGAATAAGAATCCAAAGCCCTCCCGAGAAGGTGTTCGAGTATTTTGCATCTATTCACACAGTGGACGGAGAAATTTTCATGACACCATCGGATCTAATGAGAGCGATTGTTCCTGTGTTTCCTCCCTCGGAGTCGAATCGAGTAAGAGAAGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTCTATTGTGTTCCTTCTAAATTCTTCATGCTTTTTGACACCAACAATGATGGACTCATCTCCTTTGCAGAGTATAAAGCTCAACCCTTTTCCTTGTTAAACCTAATACTCAATTTAAAGCTTAATGAACACTTTCAGGTACATCTTTTTTGTTACACTTTTGAGCATACCGGAGACGAGCTTTTCAGTGGCATTCAAAATGTTTGATATTGACAACAATGGGTTAGACAATTTGTTCTCTATTTGTGTCGTGTTTCATGTTTTGTCATGTTTTTTTTTTTTGCCATTTTTGAACCATTTTGGTGGTGGAAACAGAGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGGTTGATGAGGAAACAAAACAGGCAAGGAGCTCATCATAGAGATGGGAGAAGGCTTGGGGTGAAAGCTTGCGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGGCTCTCTTCACCATGATAAATTTGTCCAATTCTTAAGACAACTTCATGATGAGGTATGATTCCCCTTCAAATTTTATATTCTAATCTTTGAAAGGTCAAAATAAAGTTGGTTATATCGAACCAATGGGTTAGTTTCGAAAGTGATTTATAGCTTATATGTGAGATCCCACGTCGGCTGGAAGCTTGTATGTGAGATCCCACGTCGGCTGGAAGCTTGTATGTGAGATCCCACGTCGGCTGGAAGCTTGTATGTGAGATCCCACTGACGTTTGAGAGGGGATCAAAACATTCCTCTCTATGACAGACGTTTTTTAAAACCGTGAGATTGACGACGATACGTAACGGACCAAAACGGACAATATCTACTAGTGGTGAGTTTGGGTGGTTATAAATGGTATTAGAGCTAGACATCGGGCTGTGTGCTAGCAAGGACCTTGACCCCCAACGAGGTGGATTGTAAGATCCCACATTGGTTGGAGAAGAGAACAAAACATTCCTTATAAGGGTGTGGAAATCTCTCAACGAGTGGATTGTAAGATCCCACATCGGTTGGAGAAGAGAATGAAACATCTCTTGTAAGGGTGTGGAAATCTCTTAACGGGTGGATTGTAAAAGGATGTGGAAATCTCTTAACGGGTGGATTGTAAGATCCCACGTCGGTTGGAGAAGGGAACAAAACATTCTTTAGAAGGGTGTGGAAGCCTCTCCTCAGCAAACGTTTGACAAAGATATGAAACGTTCACATGTTTAGACTTATATCCTTAAACGTTCGAATTCCTTAGCTTAACAAAAGAAGAGCGGATTACGCCGTTCGTGATTCGAACACCAAAACTTTTGAATTGGGTGATTTTGCAGATACTTAAACTAGAGTTCTCACATTATGATTTTAAGTCACGAGGAAGCATATCAGCCAAAGACTTCGCACTCTCCATCGTTGCATCAGCCGACATAAACCACATAGACAAACTCCTCGATCGAGTCGAAGCATTAAACAAAGAACACCATTTCAAAAACATCCGAATCACATACGAAGAGTTCAAACACTTTGCTGAGCTACGTAAGAAGCTAGAACCCTTCTCATTGGCCATTTTCAGCTATGGAAAAGTAAATGGAGAGCTCACAAAACAAGATTTCCAACGGGCCGCTTCCCACGTAAGTATCACGTGACAATATTTGCTATAACCACCGCATCTAAATGATGGTGAAAGTTATGTGTTTATAATAATTTAGAGGAAAGAGCCATACATGATAACTATTGTTATTACCGTCAAATATCTAGGGTTTAGAGTTTATTCCTCGCTATTACAGTCAAATATCCTGGGTTTAGGGTTTATTCCTCGCTATTACAGTCAAATATCTAGGGTTTAGGGTTTATTCCTCGCTCTTACCGTCAAATATCTAGGGTTTAGGGTTTATTCCTCGCTCTTACAGTCAAATATCTAGGGTTTAGGGTCTATTCCTCGCTCTTACAGTCAAATATCTAGGGTTTAGGGTCTATTCCTCGTTATTACAGTCAAATATCTAGGGTTTAGGGTTTATTCCTCGCTGTTACAGTCAAATATCTAGGGTTTAGGGTCTATTCCTCGCTCTTACAGTCAAATGATCCGCTTGCTCTAGGGTCTATTCCTCGCTCTTACACCAAAATATCAGGTAAATTCTCGCTCTTACCCCAAGGGCGCCATATTCTAGGAGCCCACCTCTATTTGTTGCGGGTCGAGGACACTTTCTCCTTCCCCTTTTCCCCAAGGGCGCCATATTCTAGGAGCCCACCTCTATTTGTTGCGGGTCGAGGACACTTTCTCCTTCCCCTTTTTCAAACTTCGATTCGTATTGTTCATAGAGAAATTTTTGGGGATTTTTGGGTTTGGGCCGAAAAAGCAATGTCACTTGATCATTATCAAATCGGCTATAATCTTTTTCTGTCCGTGAGGATCCCACAACAAAGTGCCTTGGCTACTTTTAACAAGCCACGAACTAGATCATAATCATTCTCAACTAGTCCATAAAAAGTGATCCCATTTTTTTTTTCATTGGGTCCAGATATAGACCAAAGGTCTTACGAACGATCCGACAAATATTTGCATGTACTAAACCCTAAACCCTAGATATTTGACTTTTTACTGTAATAAAAAGTCAAATATGATACCAAATCTGATGATTCTTGTGGCTTTTGGGAGCAAGGTCTGTGGAGTTTCGATCACGTCCAATGTCGTGGACATAATATTTCACATATTTGATGCAAATGGCGATGGCGACTTGAGCTCGGATGAGTTTGTGAGAGTTATTCAGCGAAGGGAAGTCAGTTCTTCGCAAGCGGCTTTGGGCGTGTCGGGGTTTTTGTCGTGCTGGTTCAGCTGTGCGGCTCGATGCTCTTCTGCAAAGCTCTTTGTTCGCAGCTGAGTTCAAGAGATATCCATGGCTGCCTCAGCTTACTGTAAATTTTTCAACATGACCTAATACTTTTCTGTGTCGTTTTCTTTAGGAGTTTATGAATGTAATCCAAGCTGTTCTTTTACAAGTTTGATCTAAATAAAGAACCACAAAAACTTTGGAAAACAAATTGTAAAATGGTGCGTGATTGATCT

mRNA sequence

TTTGAATTATAGTATGTTTTCTTTGACGTCTTTAGCTTCAAAATCTAATTCCAAATCGATCCATAAAGTTATAGCTTCACCACAACTTATCACTGTTCTATTGTCTTCTCAACGAGCTGGCTCCGATCGAGATAATAGAAGAAGCTCCATATTCGACTTCTTAACCAAATCAATTGCTTCGGGAGCTTTGATTGTCGGAGTTGGGTCGTTTTTTAGCTCAAATTTGCAAGTTTCTTTTGCTGACTTTCCGAAACAAACGACATGGACGGTGAACGAAGATCGGATTCCATACCCATTGCCGAATCAAAATGCAACGAAGAAACCAAAGTTTTTATTTGGAGATGATTACAGAAGACGAGTGTTTTTCAACTACGAAAAACGAATAAGAATCCAAAGCCCTCCCGAGAAGGTGTTCGAGTATTTTGCATCTATTCACACAGTGGACGGAGAAATTTTCATGACACCATCGGATCTAATGAGAGCGATTGTTCCTGTGTTTCCTCCCTCGGAGTCGAATCGAGTAAGAGAAGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTCTATTGTGTTCCTTCTAAATTCTTCATGCTTTTTGACACCAACAATGATGGACTCATCTCCTTTGCAGAGTACATCTTTTTTGTTACACTTTTGAGCATACCGGAGACGAGCTTTTCAGTGGCATTCAAAATGTTTGATATTGACAACAATGGAGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGGTTGATGAGGAAACAAAACAGGCAAGGAGCTCATCATAGAGATGGGAGAAGGCTTGGGGTGAAAGCTTGCGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGGCTCTCTTCACCATGATAAATTTGTCCAATTCTTAAGACAACTTCATGATGAGATACTTAAACTAGAGTTCTCACATTATGATTTTAAGTCACGAGGAAGCATATCAGCCAAAGACTTCGCACTCTCCATCGTTGCATCAGCCGACATAAACCACATAGACAAACTCCTCGATCGAGTCGAAGCATTAAACAAAGAACACCATTTCAAAAACATCCGAATCACATACGAAGAGTTCAAACACTTTGCTGAGCTACGTAAGAAGCTAGAACCCTTCTCATTGGCCATTTTCAGCTATGGAAAAGTAAATGGAGAGCTCACAAAACAAGATTTCCAACGGGCCGCTTCCCACGTCTGTGGAGTTTCGATCACGTCCAATGTCGTGGACATAATATTTCACATATTTGATGCAAATGGCGATGGCGACTTGAGCTCGGATGAGTTTGTGAGAGTTATTCAGCGAAGGGAAGTCAGTTCTTCGCAAGCGGCTTTGGGCGTGTCGGGGTTTTTGTCGTGCTGGTTCAGCTGTGCGGCTCGATGCTCTTCTGCAAAGCTCTTTGTTCGCAGCTGAGTTCAAGAGATATCCATGGCTGCCTCAGCTTACTGTAAATTTTTCAACATGACCTAATACTTTTCTGTGTCGTTTTCTTTAGGAGTTTATGAATGTAATCCAAGCTGTTCTTTTACAAGTTTGATCTAAATAAAGAACCACAAAAACTTTGGAAAACAAATTGTAAAATGGTGCGTGATTGATCT

Coding sequence (CDS)

ATGTTTTCTTTGACGTCTTTAGCTTCAAAATCTAATTCCAAATCGATCCATAAAGTTATAGCTTCACCACAACTTATCACTGTTCTATTGTCTTCTCAACGAGCTGGCTCCGATCGAGATAATAGAAGAAGCTCCATATTCGACTTCTTAACCAAATCAATTGCTTCGGGAGCTTTGATTGTCGGAGTTGGGTCGTTTTTTAGCTCAAATTTGCAAGTTTCTTTTGCTGACTTTCCGAAACAAACGACATGGACGGTGAACGAAGATCGGATTCCATACCCATTGCCGAATCAAAATGCAACGAAGAAACCAAAGTTTTTATTTGGAGATGATTACAGAAGACGAGTGTTTTTCAACTACGAAAAACGAATAAGAATCCAAAGCCCTCCCGAGAAGGTGTTCGAGTATTTTGCATCTATTCACACAGTGGACGGAGAAATTTTCATGACACCATCGGATCTAATGAGAGCGATTGTTCCTGTGTTTCCTCCCTCGGAGTCGAATCGAGTAAGAGAAGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTCTATTGTGTTCCTTCTAAATTCTTCATGCTTTTTGACACCAACAATGATGGACTCATCTCCTTTGCAGAGTACATCTTTTTTGTTACACTTTTGAGCATACCGGAGACGAGCTTTTCAGTGGCATTCAAAATGTTTGATATTGACAACAATGGAGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGGTTGATGAGGAAACAAAACAGGCAAGGAGCTCATCATAGAGATGGGAGAAGGCTTGGGGTGAAAGCTTGCGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGGCTCTCTTCACCATGATAAATTTGTCCAATTCTTAAGACAACTTCATGATGAGATACTTAAACTAGAGTTCTCACATTATGATTTTAAGTCACGAGGAAGCATATCAGCCAAAGACTTCGCACTCTCCATCGTTGCATCAGCCGACATAAACCACATAGACAAACTCCTCGATCGAGTCGAAGCATTAAACAAAGAACACCATTTCAAAAACATCCGAATCACATACGAAGAGTTCAAACACTTTGCTGAGCTACGTAAGAAGCTAGAACCCTTCTCATTGGCCATTTTCAGCTATGGAAAAGTAAATGGAGAGCTCACAAAACAAGATTTCCAACGGGCCGCTTCCCACGTCTGTGGAGTTTCGATCACGTCCAATGTCGTGGACATAATATTTCACATATTTGATGCAAATGGCGATGGCGACTTGAGCTCGGATGAGTTTGTGAGAGTTATTCAGCGAAGGGAAGTCAGTTCTTCGCAAGCGGCTTTGGGCGTGTCGGGGTTTTTGTCGTGCTGGTTCAGCTGTGCGGCTCGATGCTCTTCTGCAAAGCTCTTTGTTCGCAGCTGA

Protein sequence

MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALIVGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS
Homology
BLAST of Carg17447 vs. NCBI nr
Match: KAG7021189.1 (Calcium uptake protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 943.0 bits (2436), Expect = 1.0e-270
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI 60
           MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI
Sbjct: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI 60

Query: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120
           VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY
Sbjct: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120

Query: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180
           EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR
Sbjct: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180

Query: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240
           VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR
Sbjct: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240

Query: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300
           EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR
Sbjct: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300

Query: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360
           QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR
Sbjct: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360

Query: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420
           ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH
Sbjct: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420

Query: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 477
           IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS
Sbjct: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 476

BLAST of Carg17447 vs. NCBI nr
Match: KAG6586343.1 (Calcium uptake protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 941.4 bits (2432), Expect = 3.0e-270
Identity = 475/476 (99.79%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI 60
           MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQ+AGSDRDNRRSSIFDFLTKSIASGALI
Sbjct: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQQAGSDRDNRRSSIFDFLTKSIASGALI 60

Query: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120
           VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY
Sbjct: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120

Query: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180
           EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR
Sbjct: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180

Query: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240
           VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR
Sbjct: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240

Query: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300
           EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR
Sbjct: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300

Query: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360
           QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR
Sbjct: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360

Query: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420
           ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH
Sbjct: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420

Query: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 477
           IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS
Sbjct: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 476

BLAST of Carg17447 vs. NCBI nr
Match: XP_022937835.1 (calcium uptake protein, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 935.3 bits (2416), Expect = 2.1e-268
Identity = 471/476 (98.95%), Postives = 474/476 (99.58%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI 60
           MFSLTSLASKSNSKSIH+VIASPQLITVLLSSQ+AGSDRDNRRSSIFDFLTKSIASGALI
Sbjct: 1   MFSLTSLASKSNSKSIHEVIASPQLITVLLSSQQAGSDRDNRRSSIFDFLTKSIASGALI 60

Query: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120
           VGVGSFFSSNLQVSFADFPKQTTWTVNEDR PYPLPNQNATKKPKFLFGDDYRRRVFFNY
Sbjct: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRFPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120

Query: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180
           EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR
Sbjct: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180

Query: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240
           VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR
Sbjct: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240

Query: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300
           EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR
Sbjct: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300

Query: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360
           QLHDEILKLEFSHYDFKS+GSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR
Sbjct: 301 QLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360

Query: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420
           ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH
Sbjct: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420

Query: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 477
           IFDANGDGDLSSDEFVRVIQRREVSSSQAALG SGFLSCWFSCAARCSSAKLFVRS
Sbjct: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGASGFLSCWFSCAARCSSAKLFVRS 476

BLAST of Carg17447 vs. NCBI nr
Match: XP_022965722.1 (calcium uptake protein, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 912.1 bits (2356), Expect = 1.9e-261
Identity = 460/476 (96.64%), Postives = 468/476 (98.32%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI 60
           MFSLTSLASKSNSKSIHK IAS QLITV  SSQ+AGS+RDNRRSSIFDFLTKSIASGALI
Sbjct: 1   MFSLTSLASKSNSKSIHKFIASRQLITVSFSSQQAGSERDNRRSSIFDFLTKSIASGALI 60

Query: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120
           VGVGS FSSNLQVSFADF KQTTWTVNEDR PYPLPNQNATKKP+FLFGDDYRRRVFFNY
Sbjct: 61  VGVGSLFSSNLQVSFADFSKQTTWTVNEDRFPYPLPNQNATKKPRFLFGDDYRRRVFFNY 120

Query: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180
           EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR
Sbjct: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180

Query: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240
           VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPE+SFSVAFKMFDIDNNGEIDR
Sbjct: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDR 240

Query: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300
           EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR
Sbjct: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300

Query: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360
           QLHDEILKLEFSHYDFKS+GSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR
Sbjct: 301 QLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360

Query: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420
           ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS VCGVSIT+NVVDIIFH
Sbjct: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQVCGVSITANVVDIIFH 420

Query: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 477
           IFDANGDGDLSS+EFVRVIQRREV+SSQAALGVSGFLSCWFSCAARCSSAKLFVRS
Sbjct: 421 IFDANGDGDLSSNEFVRVIQRREVNSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 476

BLAST of Carg17447 vs. NCBI nr
Match: XP_023538495.1 (calcium uptake protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023538497.1 calcium uptake protein, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 900.6 bits (2326), Expect = 5.8e-258
Identity = 459/478 (96.03%), Postives = 465/478 (97.28%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHK-VIAS-PQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGA 60
           MFSLTSLASKSNSKSIHK +IAS PQLI       +AGSDRDNRRSSIFDFLTKSIASGA
Sbjct: 1   MFSLTSLASKSNSKSIHKFIIASPPQLI-------QAGSDRDNRRSSIFDFLTKSIASGA 60

Query: 61  LIVGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFF 120
           LIVGVGSFFSSNLQVSFADFPKQTTWTVNEDR PYPLPNQNATKKP+FLFGDDYRRRVFF
Sbjct: 61  LIVGVGSFFSSNLQVSFADFPKQTTWTVNEDRFPYPLPNQNATKKPRFLFGDDYRRRVFF 120

Query: 121 NYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGE 180
           NYEKRIRIQSPPEKVFEYFASIHT DGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGE
Sbjct: 121 NYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGE 180

Query: 181 RRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEI 240
           RRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPE+SFSVAFKMFDIDNNGEI
Sbjct: 181 RRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEI 240

Query: 241 DREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQF 300
           DREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQF
Sbjct: 241 DREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQF 300

Query: 301 LRQLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKN 360
           LRQLHDEILKLEFSHYDFKS+GSISAKDFALSIVA+ADINHIDKLLDRVEALNKEHHFKN
Sbjct: 301 LRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRVEALNKEHHFKN 360

Query: 361 IRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDII 420
           IRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS VCGVSITSNVVDII
Sbjct: 361 IRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQVCGVSITSNVVDII 420

Query: 421 FHIFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 477
           FHIFDANGDGDLSSDEFVRVIQRREVSSSQA LGVSGFLSCWFSCAARCSSAKLFVRS
Sbjct: 421 FHIFDANGDGDLSSDEFVRVIQRREVSSSQATLGVSGFLSCWFSCAARCSSAKLFVRS 471

BLAST of Carg17447 vs. ExPASy Swiss-Prot
Match: Q9SZ45 (Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.4e-134
Identity = 240/368 (65.22%), Postives = 300/368 (81.52%), Query Frame = 0

Query: 106 FLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPS 165
           F+FGD YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T  GEI M P+DLMRAIVPVFPPS
Sbjct: 132 FIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPS 191

Query: 166 ESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSV 225
           ES+ VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPE+SF+V
Sbjct: 192 ESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAV 251

Query: 226 AFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLG--VKACVENGGLVEYFFG 285
           AFKMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G  +   VE+GGLVEYFFG
Sbjct: 252 AFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFG 311

Query: 286 QDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKL 345
           +DG+  L HDKF +F++ L +E+L+LEF+HYD+K RGSISAKDFALS+VA+AD +H+ KL
Sbjct: 312 KDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKL 371

Query: 346 LDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS 405
           LDRVE+L++  H +++RI+ +EFK F ELR KL PFSLA+F+YGK NG LT +DF+RAAS
Sbjct: 372 LDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAAS 431

Query: 406 HVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRREVSSSQ-AALGVSGFLSCWFS 465
            VCG++++ NV++I FH+FD+N DG+LS DEF+RV+ RRE   +Q  A G+S + S  + 
Sbjct: 432 QVCGITLSDNVIEIAFHVFDSNQDGNLSVDEFLRVLHRRERDVAQPIAKGLSRYFSDGWK 491

Query: 466 CAARCSSA 471
            +  CSS+
Sbjct: 492 GSKNCSSS 498

BLAST of Carg17447 vs. ExPASy Swiss-Prot
Match: Q6P6Q9 (Calcium uptake protein 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Micu1 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 6.7e-47
Identity = 120/388 (30.93%), Postives = 197/388 (50.77%), Query Frame = 0

Query: 89  DRIPYPLPNQNATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTV----D 148
           D    P P +   K+  F      R R    YE RIR  S P+K+F YFA++  +    +
Sbjct: 91  DTAAEPYPEEKKKKRSGF------RDRKVMEYENRIRAYSTPDKIFRYFATLKVINEPGE 150

Query: 149 GEIFMTPSDLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDG 208
            E+FMTP D +R+I P     E   + +  ++    +++  E      +  + +     G
Sbjct: 151 TEVFMTPQDFVRSITPNEKQPEHLGLDQYIIKRFDGKKIAQEREKFADEGSIFYTLGECG 210

Query: 209 LISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRD 268
           LISF++YIF  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD
Sbjct: 211 LISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRD 270

Query: 269 ----GRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKS 328
               G  L    C     L  YFFG D  G L    F++F R+L  ++LKLEF  +D   
Sbjct: 271 RPTTGNTLKSGLC---SALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PV 330

Query: 329 RGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEP 388
            G IS + F   ++A + +    K L  ++   K+H      +T++E ++F    K +  
Sbjct: 331 DGRISERQFGGMLLAYSGVQ--SKKLTAMQRQLKKHFKDGKGLTFQEVENFFTFLKNIND 390

Query: 389 FSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRV 448
              A+  Y      L K   Q+ A  V  V ++ +V D++F +FD +G+G+LS+ EFV +
Sbjct: 391 VDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSI 450

Query: 449 IQRREVSSSQAA--LGVSGFLSCWFSCA 465
           +++R +   +    +G +  +   + CA
Sbjct: 451 MKQRLMRGLEKPKDMGFTRLMQAMWKCA 466

BLAST of Carg17447 vs. ExPASy Swiss-Prot
Match: Q8VCX5 (Calcium uptake protein 1, mitochondrial OS=Mus musculus OX=10090 GN=Micu1 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 8.7e-47
Identity = 120/388 (30.93%), Postives = 196/388 (50.52%), Query Frame = 0

Query: 89  DRIPYPLPNQNATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTV----D 148
           D    P P     K+  F      R R    YE RIR  S P+K+F YFA++  +    +
Sbjct: 91  DAAAEPYPEDKKKKRSGF------RDRKVMEYENRIRAYSTPDKIFRYFATLKVINEPGE 150

Query: 149 GEIFMTPSDLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDG 208
            E+FMTP D +R+I P     E   + +  ++    +++  E      +  + +     G
Sbjct: 151 TEVFMTPQDFVRSITPNEKQPEHLGLDQYIIKRFDGKKIAQEREKFADEGSIFYSLGECG 210

Query: 209 LISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRD 268
           LISF++YIF  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD
Sbjct: 211 LISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRD 270

Query: 269 ----GRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKS 328
               G  L    C     L  YFFG D  G L    F++F R+L  ++LKLEF  +D   
Sbjct: 271 RPTTGNTLKSGLC---SALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PV 330

Query: 329 RGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEP 388
            G IS + F   ++A + +    K L  ++   K+H      +T++E ++F    K +  
Sbjct: 331 DGRISERQFGGMLLAYSGVQ--SKKLTAMQRQLKKHFKDGKGLTFQEVENFFTFLKNIND 390

Query: 389 FSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRV 448
              A+  Y      L K   Q+ A  V  V ++ +V D++F +FD +G+G+LS+ EFV +
Sbjct: 391 VDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSI 450

Query: 449 IQRREVSSSQAA--LGVSGFLSCWFSCA 465
           +++R +   +    +G +  +   + CA
Sbjct: 451 MKQRLMRGLEKPKDMGFTRLMQAMWKCA 466

BLAST of Carg17447 vs. ExPASy Swiss-Prot
Match: Q9BPX6 (Calcium uptake protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=MICU1 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.1e-46
Identity = 118/383 (30.81%), Postives = 197/383 (51.44%), Query Frame = 0

Query: 94  PLPNQNATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTV----DGEIFM 153
           P P +   K+  F      R R    YE RIR  S P+K+F YFA++  +    + E+FM
Sbjct: 94  PHPEEKKKKRSGF------RDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFM 153

Query: 154 TPSDLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFA 213
           TP D +R+I P     E   + +  ++    +++  E      +  + +     GLISF+
Sbjct: 154 TPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFS 213

Query: 214 EYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRD----G 273
           +YIF  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    G
Sbjct: 214 DYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTG 273

Query: 274 RRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSIS 333
             L    C     L  YFFG D  G L    F++F R+L  ++LKLEF  +D    G I+
Sbjct: 274 NTLKSGLC---SALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRIT 333

Query: 334 AKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAI 393
            + F   ++A + +    K L  ++   K+H  +   +T++E ++F    K +     A+
Sbjct: 334 ERQFGGMLLAYSGVQ--SKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTAL 393

Query: 394 FSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRRE 453
             Y      L K   Q+ A  V  V ++ +V D++F +FD +G+G+LS+ EFV ++++R 
Sbjct: 394 SFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRL 453

Query: 454 VSSSQAA--LGVSGFLSCWFSCA 465
           +   +    +G +  +   + CA
Sbjct: 454 MRGLEKPKDMGFTRLMQAMWKCA 464

BLAST of Carg17447 vs. ExPASy Swiss-Prot
Match: Q4R518 (Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 PE=2 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 1.1e-46
Identity = 118/383 (30.81%), Postives = 197/383 (51.44%), Query Frame = 0

Query: 94  PLPNQNATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTV----DGEIFM 153
           P P +   K+  F      R R    YE RIR  S P+K+F YFA++  +    + E+FM
Sbjct: 94  PHPEEKKKKRSGF------RDRKVMEYENRIRAYSTPDKIFRYFATLKVINEPGEAEVFM 153

Query: 154 TPSDLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFA 213
           TP D +R+I P     E   + +  ++    +++  E      +  + +     GLISF+
Sbjct: 154 TPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFS 213

Query: 214 EYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRD----G 273
           +YIF  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    G
Sbjct: 214 DYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTG 273

Query: 274 RRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSIS 333
             L    C     L  YFFG D  G L    F++F R+L  ++LKLEF  +D    G I+
Sbjct: 274 NTLKSGLC---SALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRIT 333

Query: 334 AKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAI 393
            + F   ++A + +    K L  ++   K+H  +   +T++E ++F    K +     A+
Sbjct: 334 ERQFGGMLLAYSGVQ--SKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTAL 393

Query: 394 FSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRRE 453
             Y      L K   Q+ A  V  V ++ +V D++F +FD +G+G+LS+ EFV ++++R 
Sbjct: 394 SFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRL 453

Query: 454 VSSSQAA--LGVSGFLSCWFSCA 465
           +   +    +G +  +   + CA
Sbjct: 454 MRGLEKPKDMGFTRLMQAMWKCA 464

BLAST of Carg17447 vs. ExPASy TrEMBL
Match: A0A6J1FHX4 (calcium uptake protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111444105 PE=4 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.0e-268
Identity = 471/476 (98.95%), Postives = 474/476 (99.58%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI 60
           MFSLTSLASKSNSKSIH+VIASPQLITVLLSSQ+AGSDRDNRRSSIFDFLTKSIASGALI
Sbjct: 1   MFSLTSLASKSNSKSIHEVIASPQLITVLLSSQQAGSDRDNRRSSIFDFLTKSIASGALI 60

Query: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120
           VGVGSFFSSNLQVSFADFPKQTTWTVNEDR PYPLPNQNATKKPKFLFGDDYRRRVFFNY
Sbjct: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRFPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120

Query: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180
           EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR
Sbjct: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180

Query: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240
           VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR
Sbjct: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240

Query: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300
           EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR
Sbjct: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300

Query: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360
           QLHDEILKLEFSHYDFKS+GSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR
Sbjct: 301 QLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360

Query: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420
           ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH
Sbjct: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420

Query: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 477
           IFDANGDGDLSSDEFVRVIQRREVSSSQAALG SGFLSCWFSCAARCSSAKLFVRS
Sbjct: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGASGFLSCWFSCAARCSSAKLFVRS 476

BLAST of Carg17447 vs. ExPASy TrEMBL
Match: A0A6J1HL37 (calcium uptake protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111465533 PE=4 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 9.4e-262
Identity = 460/476 (96.64%), Postives = 468/476 (98.32%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGSDRDNRRSSIFDFLTKSIASGALI 60
           MFSLTSLASKSNSKSIHK IAS QLITV  SSQ+AGS+RDNRRSSIFDFLTKSIASGALI
Sbjct: 1   MFSLTSLASKSNSKSIHKFIASRQLITVSFSSQQAGSERDNRRSSIFDFLTKSIASGALI 60

Query: 61  VGVGSFFSSNLQVSFADFPKQTTWTVNEDRIPYPLPNQNATKKPKFLFGDDYRRRVFFNY 120
           VGVGS FSSNLQVSFADF KQTTWTVNEDR PYPLPNQNATKKP+FLFGDDYRRRVFFNY
Sbjct: 61  VGVGSLFSSNLQVSFADFSKQTTWTVNEDRFPYPLPNQNATKKPRFLFGDDYRRRVFFNY 120

Query: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180
           EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR
Sbjct: 121 EKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERR 180

Query: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAFKMFDIDNNGEIDR 240
           VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPE+SFSVAFKMFDIDNNGEIDR
Sbjct: 181 VHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDR 240

Query: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300
           EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR
Sbjct: 241 EEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLR 300

Query: 301 QLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360
           QLHDEILKLEFSHYDFKS+GSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR
Sbjct: 301 QLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRVEALNKEHHFKNIR 360

Query: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHVCGVSITSNVVDIIFH 420
           ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS VCGVSIT+NVVDIIFH
Sbjct: 361 ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQVCGVSITANVVDIIFH 420

Query: 421 IFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 477
           IFDANGDGDLSS+EFVRVIQRREV+SSQAALGVSGFLSCWFSCAARCSSAKLFVRS
Sbjct: 421 IFDANGDGDLSSNEFVRVIQRREVNSSQAALGVSGFLSCWFSCAARCSSAKLFVRS 476

BLAST of Carg17447 vs. ExPASy TrEMBL
Match: A0A6J1CPF9 (calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111013100 PE=4 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.9e-222
Identity = 401/499 (80.36%), Postives = 433/499 (86.77%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLI------------------TVLLSSQRAGSDRDNR 60
           MFS T L SKS S SIH+V+ S +L                   ++L SS   GS  +++
Sbjct: 1   MFSSTLLVSKS-SASIHRVVTSQRLTVRLFNNQLGGSASTSPSSSLLASSHNTGSGEESK 60

Query: 61  RSSIFDFLTKSIASGALIVGVGSFF-----SSNLQVSFADFPKQTTWTVNEDRIPYPLPN 120
           RSSIFDFL KS++SG + VG+GS       +SN  VSFADF K+T WT+ ED+  YP PN
Sbjct: 61  RSSIFDFLVKSVSSGVVAVGIGSLLCLNSSTSNSNVSFADFQKETKWTMKEDQFQYPFPN 120

Query: 121 QNATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRA 180
           QNA KK KFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS+HT +GEIFMTP+DLMRA
Sbjct: 121 QNARKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASVHTPEGEIFMTPADLMRA 180

Query: 181 IVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLS 240
           IVPVFPPSESNRVREGFLRGE R+HGELYCVPS+FFMLFD N+DGLISFAEYIFFVTLLS
Sbjct: 181 IVPVFPPSESNRVREGFLRGE-RIHGELYCVPSEFFMLFDINSDGLISFAEYIFFVTLLS 240

Query: 241 IPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGL 300
           IPE+SFSVAFKMFDIDNNGEIDREEFKKVM LMRKQNRQGA HRDGRRLG+K CVENGGL
Sbjct: 241 IPESSFSVAFKMFDIDNNGEIDREEFKKVMALMRKQNRQGARHRDGRRLGMKVCVENGGL 300

Query: 301 VEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADI 360
           VEYFFGQDG GSLHHDKFVQFLRQLHDEIL+LEFSHYDFKS+GSISAKDFALS+VASADI
Sbjct: 301 VEYFFGQDGKGSLHHDKFVQFLRQLHDEILRLEFSHYDFKSQGSISAKDFALSMVASADI 360

Query: 361 NHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQD 420
           NHIDKLLDRVEALNKE  F+NIRITYEEFK FAELRKKLE FSLAIFSYGKVNGELTKQD
Sbjct: 361 NHIDKLLDRVEALNKEPQFRNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQD 420

Query: 421 FQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRREVSSSQAALGVSGFL 477
           FQRAASHVCGVSIT NVVDIIFHIFDANGDGDLSSDEFVRVIQR+EVSSSQAA+G  GFL
Sbjct: 421 FQRAASHVCGVSITRNVVDIIFHIFDANGDGDLSSDEFVRVIQRKEVSSSQAAVGAGGFL 480

BLAST of Carg17447 vs. ExPASy TrEMBL
Match: A0A1S3C4U2 (calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 6.0e-216
Identity = 403/506 (79.64%), Postives = 425/506 (83.99%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGS----------------------D 60
           MFS T L SKS SKSI +VI S  L    L++Q  GS                       
Sbjct: 1   MFSSTFLVSKS-SKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCG 60

Query: 61  RDNRRSSIFDFLTKSIASGALIVGVGSFFS-----SNLQVSFADFPKQTTW-TVNEDRIP 120
            +NRR+ +F+FL K I+SG LI GVGS  S     SN  +SFADFPK+TTW TV ED+  
Sbjct: 61  GENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQ 120

Query: 121 YPLPNQ--NATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMT 180
           YP PNQ  NA KK KFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT +GEI+M 
Sbjct: 121 YPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMR 180

Query: 181 PSDLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYI 240
           PSDLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEYI
Sbjct: 181 PSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYI 240

Query: 241 FFVTLLSIPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKA 300
           FFVTLLSIPE+SFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR G+K 
Sbjct: 241 FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV 300

Query: 301 CVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALS 360
            VENGGLVEYFFG+DG GSLHHDKFVQFLRQLH+EIL LEFSHYDFKS+GSIS KDFALS
Sbjct: 301 SVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALS 360

Query: 361 IVASADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVN 420
           IVASADINHIDKLLDRVEALNKE HFKNIRITYEEFK FAELRKKLE FSLAIFSYGKVN
Sbjct: 361 IVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVN 420

Query: 421 GELTKQDFQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRREVSSSQAA 477
           GELTKQDFQRAAS VCGVSIT NVVDIIFHIFDAN DGDLSSDEFVRVIQRREV+SSQ A
Sbjct: 421 GELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPA 480

BLAST of Carg17447 vs. ExPASy TrEMBL
Match: A0A5A7VDG3 (Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001000 PE=4 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.7e-215
Identity = 402/506 (79.45%), Postives = 425/506 (83.99%), Query Frame = 0

Query: 1   MFSLTSLASKSNSKSIHKVIASPQLITVLLSSQRAGS----------------------D 60
           MFS T L SKS SKSI +VI S  L    L++Q  GS                       
Sbjct: 1   MFSSTFLVSKS-SKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCG 60

Query: 61  RDNRRSSIFDFLTKSIASGALIVGVGSFFS-----SNLQVSFADFPKQTTW-TVNEDRIP 120
            +NRR+ +F+FL K I+SG LI GVGS  S     SN  +SFA+FPK+TTW TV ED+  
Sbjct: 61  GENRRNFVFEFLIKLISSGILIGGVGSLLSSNSSTSNSHLSFAEFPKETTWTTVKEDQFQ 120

Query: 121 YPLPNQ--NATKKPKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMT 180
           YP PNQ  NA KK KFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT +GEI+M 
Sbjct: 121 YPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMR 180

Query: 181 PSDLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYI 240
           PSDLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEYI
Sbjct: 181 PSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYI 240

Query: 241 FFVTLLSIPETSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKA 300
           FFVTLLSIPE+SFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR G+K 
Sbjct: 241 FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV 300

Query: 301 CVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALS 360
            VENGGLVEYFFG+DG GSLHHDKFVQFLRQLH+EIL LEFSHYDFKS+GSIS KDFALS
Sbjct: 301 SVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALS 360

Query: 361 IVASADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVN 420
           IVASADINHIDKLLDRVEALNKE HFKNIRITYEEFK FAELRKKLE FSLAIFSYGKVN
Sbjct: 361 IVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVN 420

Query: 421 GELTKQDFQRAASHVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRREVSSSQAA 477
           GELTKQDFQRAAS VCGVSIT NVVDIIFHIFDAN DGDLSSDEFVRVIQRREV+SSQ A
Sbjct: 421 GELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPA 480

BLAST of Carg17447 vs. TAIR 10
Match: AT4G32060.1 (calcium-binding EF hand family protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.0e-135
Identity = 240/368 (65.22%), Postives = 300/368 (81.52%), Query Frame = 0

Query: 106 FLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPS 165
           F+FGD YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T  GEI M P+DLMRAIVPVFPPS
Sbjct: 132 FIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPS 191

Query: 166 ESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSV 225
           ES+ VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPE+SF+V
Sbjct: 192 ESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAV 251

Query: 226 AFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLG--VKACVENGGLVEYFFG 285
           AFKMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G  +   VE+GGLVEYFFG
Sbjct: 252 AFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFG 311

Query: 286 QDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKL 345
           +DG+  L HDKF +F++ L +E+L+LEF+HYD+K RGSISAKDFALS+VA+AD +H+ KL
Sbjct: 312 KDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKL 371

Query: 346 LDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS 405
           LDRVE+L++  H +++RI+ +EFK F ELR KL PFSLA+F+YGK NG LT +DF+RAAS
Sbjct: 372 LDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAAS 431

Query: 406 HVCGVSITSNVVDIIFHIFDANGDGDLSSDEFVRVIQRREVSSSQ-AALGVSGFLSCWFS 465
            VCG++++ NV++I FH+FD+N DG+LS DEF+RV+ RRE   +Q  A G+S + S  + 
Sbjct: 432 QVCGITLSDNVIEIAFHVFDSNQDGNLSVDEFLRVLHRRERDVAQPIAKGLSRYFSDGWK 491

Query: 466 CAARCSSA 471
            +  CSS+
Sbjct: 492 GSKNCSSS 498

BLAST of Carg17447 vs. TAIR 10
Match: AT4G32060.2 (calcium-binding EF hand family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-118
Identity = 209/300 (69.67%), Postives = 252/300 (84.00%), Query Frame = 0

Query: 106 FLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPS 165
           F+FGD YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T  GEI M P+DLMRAIVPVFPPS
Sbjct: 132 FIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPS 191

Query: 166 ESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSV 225
           ES+ VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPE+SF+V
Sbjct: 192 ESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAV 251

Query: 226 AFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLG--VKACVENGGLVEYFFG 285
           AFKMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G  +   VE+GGLVEYFFG
Sbjct: 252 AFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFG 311

Query: 286 QDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKL 345
           +DG+  L HDKF +F++ L +E+L+LEF+HYD+K RGSISAKDFALS+VA+AD +H+ KL
Sbjct: 312 KDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKL 371

Query: 346 LDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS 404
           LDRVE+L++  H +++RI+ +EFK F ELR KL PFSLA+F+YGK NG LT +DF+RAAS
Sbjct: 372 LDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAAS 430

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021189.11.0e-270100.00Calcium uptake protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6586343.13.0e-27099.79Calcium uptake protein, mitochondrial, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022937835.12.1e-26898.95calcium uptake protein, mitochondrial-like [Cucurbita moschata][more]
XP_022965722.11.9e-26196.64calcium uptake protein, mitochondrial-like [Cucurbita maxima][more]
XP_023538495.15.8e-25896.03calcium uptake protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
Match NameE-valueIdentityDescription
Q9SZ451.4e-13465.22Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE... [more]
Q6P6Q96.7e-4730.93Calcium uptake protein 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Micu1 P... [more]
Q8VCX58.7e-4730.93Calcium uptake protein 1, mitochondrial OS=Mus musculus OX=10090 GN=Micu1 PE=1 S... [more]
Q9BPX61.1e-4630.81Calcium uptake protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=MICU1 PE=1 SV... [more]
Q4R5181.1e-4630.81Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FHX41.0e-26898.95calcium uptake protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HL379.4e-26296.64calcium uptake protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1CPF91.9e-22280.36calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A1S3C4U26.0e-21679.64calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 ... [more]
A0A5A7VDG31.7e-21579.45Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
Match NameE-valueIdentityDescription
AT4G32060.11.0e-13565.22calcium-binding EF hand family protein [more]
AT4G32060.22.5e-11869.67calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 222..250
e-value: 2.6E-5
score: 33.7
coord: 414..442
e-value: 0.093
score: 21.8
coord: 188..216
e-value: 0.032
score: 23.4
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 226..248
e-value: 8.3E-7
score: 28.1
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 193..214
e-value: 0.0012
score: 18.6
coord: 390..441
e-value: 2.9E-7
score: 30.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 410..445
score: 11.02648
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 218..253
score: 12.979158
IPR002048EF-hand domainCDDcd00051EFhcoord: 191..247
e-value: 1.291E-11
score: 57.9429
NoneNo IPR availableGENE3D1.10.238.10coord: 127..270
e-value: 8.1E-15
score: 56.8
NoneNo IPR availableGENE3D1.10.238.10coord: 388..451
e-value: 1.0E-7
score: 33.5
NoneNo IPR availableGENE3D1.10.238.10coord: 277..385
e-value: 1.2E-6
score: 30.1
NoneNo IPR availablePANTHERPTHR12294:SF23CALCIUM UPTAKE PROTEIN, MITOCHONDRIALcoord: 54..473
NoneNo IPR availableCDDcd15900EFh_MICUcoord: 223..442
e-value: 4.03842E-43
score: 147.76
IPR039800Calcium uptake protein 1/2/3PANTHERPTHR12294EF HAND DOMAIN FAMILY A1,A2-RELATEDcoord: 54..473
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 231..243
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 423..435
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 308..440
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 191..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg17447-RACarg17447-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
biological_process GO:0006851 mitochondrial calcium ion transmembrane transport
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005509 calcium ion binding