Carg17249 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg17249
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description1,4-alpha-D-glucan glucanohydrolase
LocationCarg_Chr18: 10952946 .. 10959981 (-)
RNA-Seq ExpressionCarg17249
SyntenyCarg17249
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAATTAAAATCTATTTTAATTAACAAATATCGTCCACTCTTGTCATTTATCTTCAATCTCTTTCTCGTTTCGTTTTCGTTCTCTCTCTTCTGTTCTTCGTTCAATTTTTATTTTTATTTTTGATTTTTTTGATTTTTTTGATTTTTGGGTCTGCGGCAGAGAGGAAGTGGCAGCAGGGGGATCATGTCGACCATTGTACTCGATCCTCTTCTCTATCACTGCGGGAAAGGGAAACAGAGATTCCAACGCCGTCCTAAATTCAATCCGCTGAAATCCTGTTCTTCCTTTACTTACTGTACCAATAAGCTTCTTTACCGTGGCCGAAGGACCTTTTTCAATCGCAATTCTTGTACGCTTCCTGTTCTTAGAGCTAGCTCAACTAATGCGGGTCTCCTTGAACCCTTTGAATCGACGGATGTTTTGCTCACGGAGACGTTCTTTGTTAAGCGTACTGAAAAGGTATATGGATTATATCTTATGATATGGGATTGTTTTTGTTTTTCTTCTCATTGTTCTTCTGGTTTTATTTGTGGGATCTGTTTTGTTGCTGCTGGGAAGTGCATTTCCCTTTTGGTTCGTTAGTTTTTCACTCTCGGGATGGGAGTTTTTTTGTTTACGTTTGCTTGTTTAACTTGATTTCCCTGAGTTTGAGAGTTGGTTGTTGATTTGAGCTTGTAGTTGGAGGGGAAGATCTCCGTCGGACTAGCACAGGGAAAGAACCAGAATCAGTGGGAGCTTACTATTGGATGTAACCTTTCTGGGAAATGGATTCTTCATTGGGGTGTGTCCTTAATCGATGATAGTGGCAGGTATTTCTCGTTTTGATCCAAAACTTGAAAATTTTTTATGCTGAGTTTTTTTTTTTTGTTCATGCCTTGCCTTCATCTAAAATGGGTTGAATGAATGAACCTTGCAGCGAATGGGATCAACCTCCTAAAGAAATGATACCCCCTGGTTCAATACCGATCAAAGTATGTTAATTTAATCTCTTGATTTCAGTGTCTAGGCTGCTCTAGCATAACCTGCGAAAGTGTAGTAACAATTATATTTTCAGGATTATGCTATTGAGACACCATTGAAAAAATCATCCTCGTCTGCTTTGGGAGATGCTGTTCATGAAGTGAAGATAGACCTTGTCCCTGACAAAGCAATTGCAGCTATAAATTTTGTTTTGAAGGTTGTTATTTTCAGTATTTAACAATTGAAGACGAGTCCTATACCTCTTTGGTCGTTGATCGTGTTCGTTTCTAATATTGCCAGTCCTTTTGGTGAATGGGACTTCTTACTTTTGGTAGTGCAGGATGAAGAAACTGGAACTTGGTATCAGCACAAAGGGAGAGATTTCAAGGTGCCTCTTTTAGGCTACCATGGTGGTGATGGCAATATAGTTGGAACGAAAAAGGGCTTGAACTTATGGCCAGGTTGGGGTGTCCTTAAATAATATATAATGTTTATTTTAATTTGGCTATGCACAGCTATATATACTAAGTGATTTCCTTCAATGTATACATCTTAGAGTATCAAATTAGAAAGTAGTGCCTATGATCTGCTTGAAGTTATGTCTGAGTTCCATTTCTTGCTTCCACTGTGCACTATAGGGAATTTTATTAGTTTGTAGGTTATGATTTTGGATTGCCTGCCTCACTTCCAGTTTCCCTGGTGTTTATCTCATTCTTTCAGCTATTGTCTATCTCACGCTGGTGAGATGTTTAAATTTACTCAATTGTAGGGGCTTTGGGTCAGCTGTCCAACCTCATTGTTAAACCAGAACCAAATTCTAAAGGTCAAGAAAGCAGTGGTGAATCTGGAGATACAAAGGAGAAGAATTATTTGGAAGGATTCTACGAAGAGCTGCCTATTGTAAAAGAAGTGGCTGTTGATAACTCGGTCAGTGTTACAGTCAGGAAGTGTCCCGAAACAACCAAATATATTTTGTATTTGGAAACTGATGTACCTGGAGATGTTACTGTTCATTGGGGAGCGTGCAGAGATGAGACTAGGAAATGGGAGATTCCAGCTGCTCCTCATCCTCCAGAAACAACAGTGTTCAAGAAAAAGGCCTTGCGTACCCCACTACAGGTTCAGTGCAATTTTTTTCCCCTGAAATTTGAGTCAAACTGATTGCAAACTCATCACTGCTTATTTTGTTTATTAAGCCATCTAAAAACCATTGCATTGTCGTCCATTTTGTGAGAAAAGAGTTCATTACAAGTATATCTTTTAAAATTCATTAGAGTAATGCATATTTTAAACATTCTATTCACACATGTACTTTTCAAATCTTCATGTTTGGATTTTGTTAAAATGCTTAAGTTTTGATTTCCAGTCAAGAATACTGTATTTTTCATTATTATGAAATACCCCGTTTGCAGCTGAAAAAAGACGGGAAGGGAAGTTGTGGAATTTTCACCGTGGAAGAAGAATTTGGAGGTTTTCTTTTTGTTCTCAAGCAGAATGAGAATTCTTGGTTAAACAACCAGGGAGAGGACTTCTACATCCCCTTCTCAAGCTCTGGTAACATGCCTGTTCAAAAGAAACAGATCAAATTAGAAGATAGAGGAGTATCTATAAAATCTGGAGAAGGATCTGAGGGAGTTTCTGTCACTGCTTATACTGTTGGAATTATCAAGGAGATCAGAAACTTGGTTACTGACATTTCTTCTCAGAAGACTCCAAAGAAAAAAACCAAAGAAGCACAAGAAAGCATTCTTCAGGAAATTGAAAAACTGGCTGCTGAAGCCTATAGTATTTTCAGAAGTTCTACTCCGACTTTTACAGAGGAAATACTTGAGACTCCTAAACCTGTAGAACCACCCGTACAAATTTCCTCTGGCACAGGTTCAGGTTTTGAAATATTGTGCCAAGGGTTTAACTGGGAATCTCACAAGTCCGGAAGATGGTACGTGGAACTTAAAGAGAAAGCAGCAGAATTATCATCCCTTGGCTTCACCGTGTTATGGCTGCCACCTCCAACGGAATCTGTGTCACCAGAGGGGTACATGCCAAAGGACTTGTATAATTTAAATTCCAGGTAATTTATAAAAAATATTATAGCTTATACGACATGATAATTTTCGTATGGGTTTCTATGATTTTATTTCTTATGCGGAAACATGTAGAACCTTTTTTTAGTCCACTTCTAGATTTTCATTATCACTTGTTAAGCAACAGTATTCCAATGATAAAATTGTTGTTCCATCTCATGCATGGAGTCTAGAGAAATTTTCTCTAGTGATCAGCGAAATTTACTCCTTCCCCTCATATTTAATAAATTGAAAAATAAAAGACATCATCGTTACGGGTAGGATCTTAAATGTTTATATTTGTCAACTCTACTTTCTTCATTTCAGATATGGTAACATTGATGAGCTAAAGGATGTTGTGAAGACATTCCATGATGTTGGGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACAATTTCAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGATCGTGCAATAGTTTCAGATGATCCTCATTTTCAGGTTTGATTTTCTTTTTGTCCTCTCAGATGCCTGCACTTTAGTTTCTATCTAATTTTCCACTTATATTTTATGAAATATTTCATGATGAAATGAAAATAAAAGGAACACTAGGTAAATGTGATCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAGCACTGGACCCTTTCCAGATGTGTAAATGTAAATGTGATCATTGAACTTTTTAATATACTGCCTCGTTTATATAACATAGAGATGTCGACTTCATTTAAATATTTACATTTTTTTTTTATTAATTTTGATTACTTGGACAACAAAATTGATTATTCATTTTTTCATGTCCTGTGAAGGGTAGGGGCAACAAAAGTAGCGGAGATAACTTCCACGCTGCTCCTAACATTGATCATTCACAAGATTTTGTGAGAAATGATATCAAGGAATGGTTATGCTGGTTAAGGTTAATTCCCCGGTCAACCCTTCTTTTTTGGTTTTTTAGTCCTTTATGTGAAGACCTTATCATATTCAAATATATAAACATTCCGTAGTCTATAAAGTAGTGTCACCTGATAATCTAATAAATGGACATTGCGGTGAAAAAAGGCTTTGGACTTAGCCTAGAGGTCCCAAGTCAAATGGCCTCTCATTGCGTACGCTGCTTTCTGTGATGTATACTCTTGAGACCCTTTTTTGTATTGCTTAGTGCTGGTATGATTAATATAATTCCTCTCTTTGAACTTGTTGAACATAAAAATCTACGGTCATTTTCTAATGAAATGGTAAATGAACCAAATAAATGAATTAAGTTCACCCATGAAGGTTCCTAGGTCTCTTTGATGCTTAGAATTGATCAGAATATTTACCACAATGACCGGGTGTGGTTCTTTCAATTTTTTTTTTTTTAATGTTTGCTCCTTGAAATGAAACCATGAAGCTATCTGCATCAGAAAGGGGAAAAAACATGGAGCTATCTTTAAATTATCAATTGGTGGATTTCATTCTGGTTGTTTTAATGAAGTGCTCGATGTTCTTAACTTCTATGATTTGCTGATACGCACATGGTCATTACTATCCCGTTTAATTTGAAAGTCTCTTTTTCACCTTGCTTTCAAGAATTAGAGGCGTGTTTTTTATGCATATGCTTTTGGCTGTTGACATGCAGAAAGGAAATTGGGTATGATGGGTGGAGGCTTGATTTTGTCAGAGGATTCTGGGGTGGTTATGTGAAGGATTACTTGGATGCCAGTGAACCTTACTTTGCTGTAGGCGAATACTGGGATTCCCTTAGCTATACGTATGGTGAGATGGATCATAATCAAGATGCTCATAGACAGAGAATTGTTGACTGGATAAATGCTACCAATGGAACTGCTGGAGCATTCGATGTTACTACCAAAGGGATTCTTCATTCTGTAAGCTTTCATTTAATTATCTGGGTGTCAAATCTGTGGAAGATGTTTCCTGAGCTTATTCACAATCTAACTTGTTATTATACTCGCAAGATGTGAGATCCCACATGGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACTTCCCCCTAGTAGACACGTTTTAAAATCGTGAGGCTGACGACGATATGTAACGGGCCAAAGCGGACAATATCTACCAGCGGTGGCCTCGGACTATTACAAATGATATTAGAGTTAGACACCGGGCGGTGTACCGGTGAGAACACTAAGTCCCGAAGGGAGGTGGATTGTGAGATTCCACATCGGTTGGGGAGGAGAACAAAACCTTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAGCCTTGAGGAGAAACCTGAAAGGGAAAGTCCAAAGAGAACAATATCTAGTAGCGGTAGGCTTGAGCTGTTACACAAGAAAACGAATTGTTATTATATTTCATAATATCTGTTTTCTCCTAGTAGTATCTGTTTTCAATGTCTGATGGATTCAATTACAAAAATCATATTTTTGTTCGTTTGATGCAGTAGTTCTTCAGGTTGCAGCATTACCGTAAAATTCTTGATGATGCAAGAAATTTTTCTTGCTACTCTTAGAACGTCTTATAACCAGTTGGAAAATATCTTTTATAGGCACTTCAGAGATGTGAATATTGGCGATTATCAGATCAGAAAGGAAAGCCTCCAGGAGTTGTTGGATGGTGGCCTTCTCGTGCTGTTACCTTTATAGAGAATCATGACACTGGATCTACTCAGGTTTAGGCATCTTTCCATAAGAACTAATTTATGGCATTTAAAACTGAAACCTTCATTCGAAATGCTCTCTGATGTTTTACTCTTAATATCGTTAAACTGATCGTGATGTTCTGTACATTGCCTTATGCCATGTAGAATCTGCAATTTTCAGCAAATGTTATGCTGTTTTCTGATTTGTAGGGTCATTGGAGATTTCCTGGTGGAAAGGAAATGCAAGGGTATGCTTACATCCTGACTCATCCAGGGACACCATCAGTATTCTTTGACCACATTCTCTCCCATTACAAATCTGAAATTGCAGCACTTATTACTCTCAGACAACGAAATAAAGTTAATTGCCGGAGTGCAGTAAGTATACATGACCTTTTATGTGTTCTTATTACTTCAAAAGTTTGAAGAACTTCACTATTTTTATGAACTTTATTTTCCTCACCTTTTCACCTCCTCTGGGATTTTCATTCATTTTGGATTTGGAAACAGAATGCACTTCTCAGATTGAACATCGGCTGTTTCTTTGACTTTAGTTTCTTAGCAATCATTCGTTGGTCGCAAGACCCCGAAATTCCGATTGGTCTATGCCTTTACAGACCAAGAACAAATAAGCCTATGTTTTAATTACTTTGTACTGCATTTCTCAGGTCAAAATTCAGAAGGCAGAAAGGGATGTCTATGCAGCCACCATTGATGATAAGGTTGCAGTGAAGATCGGTCCAGGCCATTTCGAGCCTCCTAGCGTATCCGGTGGATGGTCGTTAGTTATTGAAGGAAACGATTACAAGGTTTGGGAAGTGTCCAAGTAAATTCTGGTGAGTCCCAAAATTTAGACAATAAGCAAATTGTATGAGACTTTGTGATTGTTGGATAAAGCTCATGTAAATTGTTCCAGAAGAGGCTGATTGTGGATAGTAGATTTATAGTACTGTTTTATATGTATACATATTCATTGGCTCTTAGAGAAGCATTCATTGAGGATGCTGTTAATGTTATTGTAAAAGCTTGAACGATTGATGGCTTAGCTTTGTATAAACTCTTCAGCAGGTTATAATAATACACAAGAAGCTGTGGGTGATGCATTTGCACCCACCCTTTTGATTCTTGAAATTGTGTAATTACTATATGAAATGTCGATCTCTGAACTTTACAATGAACAGAAATGGAAGAAAATAGAGAAGTAGCATTTGTAAAATAAAAGGTATAATTTATAAGAAAAATATTATCCAGAGAACCTACGAAACACAATTCTACGTTGGTTTGGGAGGAGAATAAAACTCTTTACAAGATATGGAAA

mRNA sequence

TAAAATTAAAATCTATTTTAATTAACAAATATCGTCCACTCTTGTCATTTATCTTCAATCTCTTTCTCGTTTCGTTTTCGTTCTCTCTCTTCTGTTCTTCGTTCAATTTTTATTTTTATTTTTGATTTTTTTGATTTTTTTGATTTTTGGGTCTGCGGCAGAGAGGAAGTGGCAGCAGGGGGATCATGTCGACCATTGTACTCGATCCTCTTCTCTATCACTGCGGGAAAGGGAAACAGAGATTCCAACGCCGTCCTAAATTCAATCCGCTGAAATCCTGTTCTTCCTTTACTTACTGTACCAATAAGCTTCTTTACCGTGGCCGAAGGACCTTTTTCAATCGCAATTCTTGTACGCTTCCTGTTCTTAGAGCTAGCTCAACTAATGCGGGTCTCCTTGAACCCTTTGAATCGACGGATGTTTTGCTCACGGAGACGTTCTTTGTTAAGCGTACTGAAAAGTTGGAGGGGAAGATCTCCGTCGGACTAGCACAGGGAAAGAACCAGAATCAGTGGGAGCTTACTATTGGATGTAACCTTTCTGGGAAATGGATTCTTCATTGGGGTGTGTCCTTAATCGATGATAGTGGCAGCGAATGGGATCAACCTCCTAAAGAAATGATACCCCCTGGTTCAATACCGATCAAAGATTATGCTATTGAGACACCATTGAAAAAATCATCCTCGTCTGCTTTGGGAGATGCTGTTCATGAAGTGAAGATAGACCTTGTCCCTGACAAAGCAATTGCAGCTATAAATTTTGTTTTGAAGGATGAAGAAACTGGAACTTGGTATCAGCACAAAGGGAGAGATTTCAAGGTGCCTCTTTTAGGCTACCATGGTGGTGATGGCAATATAGTTGGAACGAAAAAGGGCTTGAACTTATGGCCAGGGGCTTTGGGTCAGCTGTCCAACCTCATTGTTAAACCAGAACCAAATTCTAAAGGTCAAGAAAGCAGTGGTGAATCTGGAGATACAAAGGAGAAGAATTATTTGGAAGGATTCTACGAAGAGCTGCCTATTGTAAAAGAAGTGGCTGTTGATAACTCGGTCAGTGTTACAGTCAGGAAGTGTCCCGAAACAACCAAATATATTTTGTATTTGGAAACTGATGTACCTGGAGATGTTACTGTTCATTGGGGAGCGTGCAGAGATGAGACTAGGAAATGGGAGATTCCAGCTGCTCCTCATCCTCCAGAAACAACAGTGTTCAAGAAAAAGGCCTTGCGTACCCCACTACAGCTGAAAAAAGACGGGAAGGGAAGTTGTGGAATTTTCACCGTGGAAGAAGAATTTGGAGGTTTTCTTTTTGTTCTCAAGCAGAATGAGAATTCTTGGTTAAACAACCAGGGAGAGGACTTCTACATCCCCTTCTCAAGCTCTGGTAACATGCCTGTTCAAAAGAAACAGATCAAATTAGAAGATAGAGGAGTATCTATAAAATCTGGAGAAGGATCTGAGGGAGTTTCTGTCACTGCTTATACTGTTGGAATTATCAAGGAGATCAGAAACTTGGTTACTGACATTTCTTCTCAGAAGACTCCAAAGAAAAAAACCAAAGAAGCACAAGAAAGCATTCTTCAGGAAATTGAAAAACTGGCTGCTGAAGCCTATAGTATTTTCAGAAGTTCTACTCCGACTTTTACAGAGGAAATACTTGAGACTCCTAAACCTGTAGAACCACCCGTACAAATTTCCTCTGGCACAGGTTCAGGTTTTGAAATATTGTGCCAAGGGTTTAACTGGGAATCTCACAAGTCCGGAAGATGGTACGTGGAACTTAAAGAGAAAGCAGCAGAATTATCATCCCTTGGCTTCACCGTGTTATGGCTGCCACCTCCAACGGAATCTGTGTCACCAGAGGGGTACATGCCAAAGGACTTGTATAATTTAAATTCCAGATATGGTAACATTGATGAGCTAAAGGATGTTGTGAAGACATTCCATGATGTTGGGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACAATTTCAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGATCGTGCAATAGTTTCAGATGATCCTCATTTTCAGGGTAGGGGCAACAAAAGTAGCGGAGATAACTTCCACGCTGCTCCTAACATTGATCATTCACAAGATTTTGTGAGAAATGATATCAAGGAATGGTTATGCTGGTTAAGAAAGGAAATTGGGTATGATGGGTGGAGGCTTGATTTTGTCAGAGGATTCTGGGGTGGTTATGTGAAGGATTACTTGGATGCCAGTGAACCTTACTTTGCTGTAGGCGAATACTGGGATTCCCTTAGCTATACGTATGGTGAGATGGATCATAATCAAGATGCTCATAGACAGAGAATTGTTGACTGGATAAATGCTACCAATGGAACTGCTGGAGCATTCGATGTTACTACCAAAGGGATTCTTCATTCTGCACTTCAGAGATGTGAATATTGGCGATTATCAGATCAGAAAGGAAAGCCTCCAGGAGTTGTTGGATGGTGGCCTTCTCGTGCTGTTACCTTTATAGAGAATCATGACACTGGATCTACTCAGGGTCATTGGAGATTTCCTGGTGGAAAGGAAATGCAAGGGTATGCTTACATCCTGACTCATCCAGGGACACCATCAGTATTCTTTGACCACATTCTCTCCCATTACAAATCTGAAATTGCAGCACTTATTACTCTCAGACAACGAAATAAAGTTAATTGCCGGAGTGCAGTCAAAATTCAGAAGGCAGAAAGGGATGTCTATGCAGCCACCATTGATGATAAGGTTGCAGTGAAGATCGGTCCAGGCCATTTCGAGCCTCCTAGCGTATCCGGTGGATGGTCGTTAGTTATTGAAGGAAACGATTACAAGGTTTGGGAAGTGTCCAAGTAAATTCTGGTGAGTCCCAAAATTTAGACAATAAGCAAATTGTATGAGACTTTGTGATTGTTGGATAAAGCTCATGTAAATTGTTCCAGAAGAGGCTGATTGTGGATAGTAGATTTATAGTACTGTTTTATATGTATACATATTCATTGGCTCTTAGAGAAGCATTCATTGAGGATGCTGTTAATGTTATTGTAAAAGCTTGAACGATTGATGGCTTAGCTTTGTATAAACTCTTCAGCAGGTTATAATAATACACAAGAAGCTGTGGGTGATGCATTTGCACCCACCCTTTTGATTCTTGAAATTGTGTAATTACTATATGAAATGTCGATCTCTGAACTTTACAATGAACAGAAATGGAAGAAAATAGAGAAGTAGCATTTGTAAAATAAAAGGTATAATTTATAAGAAAAATATTATCCAGAGAACCTACGAAACACAATTCTACGTTGGTTTGGGAGGAGAATAAAACTCTTTACAAGATATGGAAA

Coding sequence (CDS)

ATGTCGACCATTGTACTCGATCCTCTTCTCTATCACTGCGGGAAAGGGAAACAGAGATTCCAACGCCGTCCTAAATTCAATCCGCTGAAATCCTGTTCTTCCTTTACTTACTGTACCAATAAGCTTCTTTACCGTGGCCGAAGGACCTTTTTCAATCGCAATTCTTGTACGCTTCCTGTTCTTAGAGCTAGCTCAACTAATGCGGGTCTCCTTGAACCCTTTGAATCGACGGATGTTTTGCTCACGGAGACGTTCTTTGTTAAGCGTACTGAAAAGTTGGAGGGGAAGATCTCCGTCGGACTAGCACAGGGAAAGAACCAGAATCAGTGGGAGCTTACTATTGGATGTAACCTTTCTGGGAAATGGATTCTTCATTGGGGTGTGTCCTTAATCGATGATAGTGGCAGCGAATGGGATCAACCTCCTAAAGAAATGATACCCCCTGGTTCAATACCGATCAAAGATTATGCTATTGAGACACCATTGAAAAAATCATCCTCGTCTGCTTTGGGAGATGCTGTTCATGAAGTGAAGATAGACCTTGTCCCTGACAAAGCAATTGCAGCTATAAATTTTGTTTTGAAGGATGAAGAAACTGGAACTTGGTATCAGCACAAAGGGAGAGATTTCAAGGTGCCTCTTTTAGGCTACCATGGTGGTGATGGCAATATAGTTGGAACGAAAAAGGGCTTGAACTTATGGCCAGGGGCTTTGGGTCAGCTGTCCAACCTCATTGTTAAACCAGAACCAAATTCTAAAGGTCAAGAAAGCAGTGGTGAATCTGGAGATACAAAGGAGAAGAATTATTTGGAAGGATTCTACGAAGAGCTGCCTATTGTAAAAGAAGTGGCTGTTGATAACTCGGTCAGTGTTACAGTCAGGAAGTGTCCCGAAACAACCAAATATATTTTGTATTTGGAAACTGATGTACCTGGAGATGTTACTGTTCATTGGGGAGCGTGCAGAGATGAGACTAGGAAATGGGAGATTCCAGCTGCTCCTCATCCTCCAGAAACAACAGTGTTCAAGAAAAAGGCCTTGCGTACCCCACTACAGCTGAAAAAAGACGGGAAGGGAAGTTGTGGAATTTTCACCGTGGAAGAAGAATTTGGAGGTTTTCTTTTTGTTCTCAAGCAGAATGAGAATTCTTGGTTAAACAACCAGGGAGAGGACTTCTACATCCCCTTCTCAAGCTCTGGTAACATGCCTGTTCAAAAGAAACAGATCAAATTAGAAGATAGAGGAGTATCTATAAAATCTGGAGAAGGATCTGAGGGAGTTTCTGTCACTGCTTATACTGTTGGAATTATCAAGGAGATCAGAAACTTGGTTACTGACATTTCTTCTCAGAAGACTCCAAAGAAAAAAACCAAAGAAGCACAAGAAAGCATTCTTCAGGAAATTGAAAAACTGGCTGCTGAAGCCTATAGTATTTTCAGAAGTTCTACTCCGACTTTTACAGAGGAAATACTTGAGACTCCTAAACCTGTAGAACCACCCGTACAAATTTCCTCTGGCACAGGTTCAGGTTTTGAAATATTGTGCCAAGGGTTTAACTGGGAATCTCACAAGTCCGGAAGATGGTACGTGGAACTTAAAGAGAAAGCAGCAGAATTATCATCCCTTGGCTTCACCGTGTTATGGCTGCCACCTCCAACGGAATCTGTGTCACCAGAGGGGTACATGCCAAAGGACTTGTATAATTTAAATTCCAGATATGGTAACATTGATGAGCTAAAGGATGTTGTGAAGACATTCCATGATGTTGGGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACAATTTCAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGATCGTGCAATAGTTTCAGATGATCCTCATTTTCAGGGTAGGGGCAACAAAAGTAGCGGAGATAACTTCCACGCTGCTCCTAACATTGATCATTCACAAGATTTTGTGAGAAATGATATCAAGGAATGGTTATGCTGGTTAAGAAAGGAAATTGGGTATGATGGGTGGAGGCTTGATTTTGTCAGAGGATTCTGGGGTGGTTATGTGAAGGATTACTTGGATGCCAGTGAACCTTACTTTGCTGTAGGCGAATACTGGGATTCCCTTAGCTATACGTATGGTGAGATGGATCATAATCAAGATGCTCATAGACAGAGAATTGTTGACTGGATAAATGCTACCAATGGAACTGCTGGAGCATTCGATGTTACTACCAAAGGGATTCTTCATTCTGCACTTCAGAGATGTGAATATTGGCGATTATCAGATCAGAAAGGAAAGCCTCCAGGAGTTGTTGGATGGTGGCCTTCTCGTGCTGTTACCTTTATAGAGAATCATGACACTGGATCTACTCAGGGTCATTGGAGATTTCCTGGTGGAAAGGAAATGCAAGGGTATGCTTACATCCTGACTCATCCAGGGACACCATCAGTATTCTTTGACCACATTCTCTCCCATTACAAATCTGAAATTGCAGCACTTATTACTCTCAGACAACGAAATAAAGTTAATTGCCGGAGTGCAGTCAAAATTCAGAAGGCAGAAAGGGATGTCTATGCAGCCACCATTGATGATAAGGTTGCAGTGAAGATCGGTCCAGGCCATTTCGAGCCTCCTAGCGTATCCGGTGGATGGTCGTTAGTTATTGAAGGAAACGATTACAAGGTTTGGGAAGTGTCCAAGTAA

Protein sequence

MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPVLRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSGKWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKIDLVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQLSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETTKYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGSCGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKSGEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFRSSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITLRQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWEVSK
Homology
BLAST of Carg17249 vs. NCBI nr
Match: KAG7013163.1 (Alpha-amylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1885.2 bits (4882), Expect = 0.0e+00
Identity = 904/904 (100.00%), Postives = 904/904 (100.00%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
           RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900

Query: 901 EVSK 905
           EVSK
Sbjct: 901 EVSK 904

BLAST of Carg17249 vs. NCBI nr
Match: XP_022945374.1 (alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1879.0 bits (4866), Expect = 0.0e+00
Identity = 901/904 (99.67%), Postives = 901/904 (99.67%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
           RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900

Query: 901 EVSK 905
           EVSK
Sbjct: 901 EVSK 904

BLAST of Carg17249 vs. NCBI nr
Match: KAG6574106.1 (Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1879.0 bits (4866), Expect = 0.0e+00
Identity = 901/904 (99.67%), Postives = 901/904 (99.67%), Query Frame = 0

Query: 1    MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
            MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 128  MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 187

Query: 61   LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
            LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 188  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 247

Query: 121  KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
            KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 248  KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 307

Query: 181  LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
            LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 308  LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 367

Query: 241  LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
            LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 368  LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 427

Query: 301  KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
            KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 428  KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 487

Query: 361  CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
            CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 488  CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 547

Query: 421  GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
            GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 548  GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 607

Query: 481  SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
            SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 608  SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 667

Query: 541  SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
            SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 668  SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 727

Query: 601  HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
            HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 728  HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 787

Query: 661  NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
            NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 788  NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 847

Query: 721  HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
            HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 848  HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 907

Query: 781  RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
            RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 908  RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 967

Query: 841  RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
            RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 968  RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 1027

Query: 901  EVSK 905
            EVSK
Sbjct: 1028 EVSK 1031

BLAST of Carg17249 vs. NCBI nr
Match: XP_022967951.1 (alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1875.5 bits (4857), Expect = 0.0e+00
Identity = 897/904 (99.23%), Postives = 901/904 (99.67%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFF+KRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWY+ELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
           RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPS+SGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSISGGWSLVIEGNDYKVW 900

Query: 901 EVSK 905
           EVSK
Sbjct: 901 EVSK 904

BLAST of Carg17249 vs. NCBI nr
Match: XP_023542260.1 (alpha-amylase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1871.3 bits (4846), Expect = 0.0e+00
Identity = 898/904 (99.34%), Postives = 899/904 (99.45%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQR PKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRCPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCRLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIP GSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPSGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
           RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900

Query: 901 EVSK 905
           EVSK
Sbjct: 901 EVSK 904

BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 604/906 (66.67%), Postives = 720/906 (79.47%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCG-KGKQRFQR-RPKFNPLKSCSSFTYCTNKLLYR-GRRTFFNRNSCT 60
           MST+ ++ LL+H   +   +  R    F P+       + +NKLL+  G+    +  + +
Sbjct: 1   MSTVPIESLLHHSYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVSSMNKS 60

Query: 61  LPVLRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCN 120
              +RA+S++  ++E  +S DV+  E F V+R EK EGKI V L + K +N WEL++GC+
Sbjct: 61  PVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WELSVGCS 120

Query: 121 LSGKWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEV 180
           + GKWILHWGVS + D+GSEWDQPP++M PPGSI IKDYAIETPLKK S    GD+  EV
Sbjct: 121 IPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEV 180

Query: 181 KIDLVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGA 240
            I+L  + ++AA+NFVLKDEETG WYQHKGRDFKVPL+     +GN++G KKG     GA
Sbjct: 181 AINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF----GA 240

Query: 241 LGQLSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCP 300
           LGQLSN+ +K +      +SS E+   +E+  L+ FYEE+PI K VA DNSVSVT RKCP
Sbjct: 241 LGQLSNIPLKQD------KSSAETDSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCP 300

Query: 301 ETTKYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDG 360
           ET+K I+ +ETD+PGDVTVHWG C++ T+KWEIP+ P+P ET++FK KALRT LQ K DG
Sbjct: 301 ETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDG 360

Query: 361 KGSCGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVS 420
            GS G+F+++ +  G  FVLK NEN+WLN +GEDFY+PF +S + PV+      E   VS
Sbjct: 361 NGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSSSPVE-----TEAAQVS 420

Query: 421 IKSGEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYS 480
               +  + VS + +T  II EIRNL  DISS K  K   KE QE+ILQEIEKLAAEAYS
Sbjct: 421 KPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 480

Query: 481 IFRSSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAA 540
           IFRS+TP F+EE +   +  +P ++ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA 
Sbjct: 481 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 540

Query: 541 ELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDV 600
           EL+SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG IDELKD VK FH VGIKVLGD 
Sbjct: 541 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDA 600

Query: 601 VLNHRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQD 660
           VLNHRCA F+NQNG+WN+FGGRLNWDDRA+V+DDPHFQGRGNKSSGDNFHAAPNIDHSQD
Sbjct: 601 VLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQD 660

Query: 661 FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYG 720
           FVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+PYFAVGEYWDSLSYTYG
Sbjct: 661 FVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG 720

Query: 721 EMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGW 780
           EMD+NQDAHRQRIVDWINAT+G AGAFDVTTKGILH+ALQ+CEYWRLSD KGKPPGVVGW
Sbjct: 721 EMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGW 780

Query: 781 WPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAAL 840
           WPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VFFDHI S Y SEIAAL
Sbjct: 781 WPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAAL 840

Query: 841 ITLRQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDY 900
           ++LR R K++CRS V I K+ERDVYAA ID+KVA+KIGPGH+EPP+ S  WS+ +EG DY
Sbjct: 841 LSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDY 887

Query: 901 KVWEVS 904
           KVWE S
Sbjct: 901 KVWETS 887

BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.4e-114
Identity = 190/395 (48.10%), Postives = 266/395 (67.34%), Query Frame = 0

Query: 510 GFEILCQGFNWESHKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 569
           G E++ Q +NWESHK   W+  L  K  +++  GFT  WLPPP++S++PEGY+P+DLY+L
Sbjct: 24  GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83

Query: 570 NSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-RLNWDDRAIV 629
           NS YG+   LK +++      ++ + D+V+NHR    +   G++N + G  L WD+ A+ 
Sbjct: 84  NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143

Query: 630 SDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 689
           S      G GN+S+GDNF+  PN+DH+Q FVR DI  WL WLR  +G+  +R DF RG+ 
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203

Query: 690 GGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 749
             YVK+Y+ A++P F+VGE WDS +Y    +D+NQD+HRQRI+ WI+AT   + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263

Query: 750 KGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 809
           KGIL  A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323

Query: 810 YAYILTHPGTPSVFFDHIL---SHYKSEIAALITLRQRNKVNCRSAVKIQKAERDVYAAT 869
           YAYILTHPG P VF+DH     S    +I  LI +R+R  ++ RS V++ KAE ++YAA 
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383

Query: 870 IDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 901
           + +K+ +K+G G + P      W+L   G+ Y VW
Sbjct: 384 VGEKICMKLGDGSWCPS--GRDWTLATSGHRYAVW 411

BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match: P00693 (Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-111
Identity = 195/410 (47.56%), Postives = 269/410 (65.61%), Query Frame = 0

Query: 505 SGTGSGFEILCQGFNWESHK-SGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMP 564
           +G  SG ++L QGFNWES K SG WY  +  K  ++++ G T +WLPPP+ SVS EGYMP
Sbjct: 19  AGLASGHQVLFQGFNWESWKQSGGWYNMMMGKVDDIAAAGVTHVWLPPPSHSVSNEGYMP 78

Query: 565 KDLYNLN-SRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIF-----G 624
             LY+++ S+YGN  ELK ++   H  G++ + D+V+NHRCA +++  GI+ IF      
Sbjct: 79  GRLYDIDASKYGNAAELKSLIGALHGKGVQAIADIVINHRCADYKDSRGIYCIFEGGTSD 138

Query: 625 GRLNWDDRAIVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGY 684
           GRL+W    I  DD  +  G  N  +G +F AAP+IDH  D V+ ++KEWL WL+ ++G+
Sbjct: 139 GRLDWGPHMICRDDTKYSDGTANLDTGADFAAAPDIDHLNDRVQRELKEWLLWLKSDLGF 198

Query: 685 DGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWIN 744
           D WRLDF RG+     K Y+D + P  AV E WD+++    G+ +++QDAHRQ +V+W++
Sbjct: 199 DAWRLDFARGYSPEMAKVYIDGTSPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVD 258

Query: 745 ATNGTAGA---FDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTG 804
              G A A   FD TTKGIL++A++  E WRL D +GK PGV+GWWP++A TF++NHDTG
Sbjct: 259 KVGGAASAGMVFDFTTKGILNAAVEG-ELWRLIDPQGKAPGVMGWWPAKAATFVDNHDTG 318

Query: 805 STQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSA 864
           STQ  W FP  K MQGYAYILTHPG P +F+DH  +  +K +IAAL+ +R+RN +   SA
Sbjct: 319 STQAMWPFPSDKVMQGYAYILTHPGIPCIFYDHFFNWGFKDQIAALVAIRKRNGITATSA 378

Query: 865 VKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
           +KI   E D Y A ID KV VKIG  +     +  G+     GNDY VWE
Sbjct: 379 LKILMHEGDAYVAEIDGKVVVKIGSRYDVGAVIPAGFVTSAHGNDYAVWE 427

BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-111
Identity = 198/398 (49.75%), Postives = 264/398 (66.33%), Query Frame = 0

Query: 513 ILCQGFNWESHKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN-S 572
           +L QGFNWES K G WY  LK    +L++ G T +WLPPP++SVSPEGY+P  LY+L+ S
Sbjct: 25  LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84

Query: 573 RYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-----RLNWDDRA 632
           +YG+ +ELK ++  FH+ GIK L D+V+NHR A+ ++  GI+ IF G     R +W    
Sbjct: 85  KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144

Query: 633 IVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVR 692
           I  DD  +  G GN  SG+ + AAP+IDH    V+ ++ EW+ WL+ EIG+DGWR DFV+
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204

Query: 693 GFWGGYVKDYLDASEPYFAVGEYWDSLSYTY-GEMDHNQDAHRQRIVDWINATNGTAGAF 752
           G+     K Y++ ++P FAVGE WDS+SY   G+ ++NQD+HR  +V+W+ +  G   AF
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAF 264

Query: 753 DVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGK 812
           D TTKGIL +A+Q  E WRL D  GKPPG++G  P  AVTFI+NHDTGSTQ  W FP  K
Sbjct: 265 DFTTKGILQAAVQG-ELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324

Query: 813 EMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSAVKIQKAERDVYA 872
            MQGYAYILTHPGTPS+F+DH      K +IA L ++R RN +N +S VKI  +E D+Y 
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYV 384

Query: 873 ATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
           A ID+K+ VKIGP       +     +   G DY VWE
Sbjct: 385 AKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 7.3e-111
Identity = 194/400 (48.50%), Postives = 261/400 (65.25%), Query Frame = 0

Query: 512 EILCQGFNWES-HKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 571
           ++L QGFNWES  K G WY  L EK  E++S G T +WLPPP+ SVSP+GYMP  LY+L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 572 -SRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIF-----GGRLNWDD 631
            S+YG   ELK +++ FHD  ++ L D+V+NHRCA +++  G++ +F      GRL+W  
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145

Query: 632 RAIVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDF 691
             I SDD  +  GRG++ +G  F AAP+IDH    V+ ++ +WL WLR ++G+DGWRLDF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205

Query: 692 VRGFWGGYVKDYLDASEPYFAVGEYWDSLSYT-YGEMDHNQDAHRQRIVDWINATNGTAG 751
            +G+     + Y+D + P F VGE W SL Y   G+   NQDA RQ +V+W+      A 
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265

Query: 752 AFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPG 811
           AFD TTKGIL +A+Q  E WRL D  GK PG++GW P +AVTF++NHDTGSTQ  W FP 
Sbjct: 266 AFDFTTKGILQAAVQG-ELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPS 325

Query: 812 GKEMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSAVKIQKAERDV 871
            K MQGYAYILTHPG P +F+DH+   + + EIA L  +R RN ++  S + I KAE D+
Sbjct: 326 DKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDI 385

Query: 872 YAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
           Y A ID KV  K+GP +     +   + +V  GNDY VWE
Sbjct: 386 YVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424

BLAST of Carg17249 vs. ExPASy TrEMBL
Match: A0A6J1G0U2 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1)

HSP 1 Score: 1879.0 bits (4866), Expect = 0.0e+00
Identity = 901/904 (99.67%), Postives = 901/904 (99.67%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
           RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900

Query: 901 EVSK 905
           EVSK
Sbjct: 901 EVSK 904

BLAST of Carg17249 vs. ExPASy TrEMBL
Match: A0A6J1HTI2 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467335 PE=3 SV=1)

HSP 1 Score: 1875.5 bits (4857), Expect = 0.0e+00
Identity = 897/904 (99.23%), Postives = 901/904 (99.67%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFF+KRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWY+ELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
           RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPS+SGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSISGGWSLVIEGNDYKVW 900

Query: 901 EVSK 905
           EVSK
Sbjct: 901 EVSK 904

BLAST of Carg17249 vs. ExPASy TrEMBL
Match: A0A6J1G0S5 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1)

HSP 1 Score: 1841.6 bits (4769), Expect = 0.0e+00
Identity = 886/904 (98.01%), Postives = 886/904 (98.01%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVS               VTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVS---------------VTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
           RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 889

Query: 901 EVSK 905
           EVSK
Sbjct: 901 EVSK 889

BLAST of Carg17249 vs. ExPASy TrEMBL
Match: A0A6J1G0P2 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1)

HSP 1 Score: 1774.2 bits (4594), Expect = 0.0e+00
Identity = 854/870 (98.16%), Postives = 855/870 (98.28%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKV 871
           RQRNKVNCRSAV I     D+    I  KV
Sbjct: 841 RQRNKVNCRSAVSI----HDLLCVLITSKV 866

BLAST of Carg17249 vs. ExPASy TrEMBL
Match: A0A6J1HS70 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111467335 PE=3 SV=1)

HSP 1 Score: 1771.1 bits (4586), Expect = 0.0e+00
Identity = 851/870 (97.82%), Postives = 855/870 (98.28%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
           MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1   MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60

Query: 61  LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
           LRASSTNAGLLEPFESTDVLLTETFF+KRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61  LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120

Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
           KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180

Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
           LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240

Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
           LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300

Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
           KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360

Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
           CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420

Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
           GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480

Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
           SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWY+ELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540

Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
           SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600

Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
           HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660

Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
           NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720

Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
           HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780

Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
           RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840

Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKV 871
           RQRNKVNCRSAV I     D+    I  KV
Sbjct: 841 RQRNKVNCRSAVSI----HDLLCVLITSKV 866

BLAST of Carg17249 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 604/906 (66.67%), Postives = 720/906 (79.47%), Query Frame = 0

Query: 1   MSTIVLDPLLYHCG-KGKQRFQR-RPKFNPLKSCSSFTYCTNKLLYR-GRRTFFNRNSCT 60
           MST+ ++ LL+H   +   +  R    F P+       + +NKLL+  G+    +  + +
Sbjct: 1   MSTVPIESLLHHSYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVSSMNKS 60

Query: 61  LPVLRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCN 120
              +RA+S++  ++E  +S DV+  E F V+R EK EGKI V L + K +N WEL++GC+
Sbjct: 61  PVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WELSVGCS 120

Query: 121 LSGKWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEV 180
           + GKWILHWGVS + D+GSEWDQPP++M PPGSI IKDYAIETPLKK S    GD+  EV
Sbjct: 121 IPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEV 180

Query: 181 KIDLVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGA 240
            I+L  + ++AA+NFVLKDEETG WYQHKGRDFKVPL+     +GN++G KKG     GA
Sbjct: 181 AINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF----GA 240

Query: 241 LGQLSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCP 300
           LGQLSN+ +K +      +SS E+   +E+  L+ FYEE+PI K VA DNSVSVT RKCP
Sbjct: 241 LGQLSNIPLKQD------KSSAETDSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCP 300

Query: 301 ETTKYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDG 360
           ET+K I+ +ETD+PGDVTVHWG C++ T+KWEIP+ P+P ET++FK KALRT LQ K DG
Sbjct: 301 ETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDG 360

Query: 361 KGSCGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVS 420
            GS G+F+++ +  G  FVLK NEN+WLN +GEDFY+PF +S + PV+      E   VS
Sbjct: 361 NGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSSSPVE-----TEAAQVS 420

Query: 421 IKSGEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYS 480
               +  + VS + +T  II EIRNL  DISS K  K   KE QE+ILQEIEKLAAEAYS
Sbjct: 421 KPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 480

Query: 481 IFRSSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAA 540
           IFRS+TP F+EE +   +  +P ++ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA 
Sbjct: 481 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 540

Query: 541 ELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDV 600
           EL+SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG IDELKD VK FH VGIKVLGD 
Sbjct: 541 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDA 600

Query: 601 VLNHRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQD 660
           VLNHRCA F+NQNG+WN+FGGRLNWDDRA+V+DDPHFQGRGNKSSGDNFHAAPNIDHSQD
Sbjct: 601 VLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQD 660

Query: 661 FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYG 720
           FVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+PYFAVGEYWDSLSYTYG
Sbjct: 661 FVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG 720

Query: 721 EMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGW 780
           EMD+NQDAHRQRIVDWINAT+G AGAFDVTTKGILH+ALQ+CEYWRLSD KGKPPGVVGW
Sbjct: 721 EMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGW 780

Query: 781 WPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAAL 840
           WPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VFFDHI S Y SEIAAL
Sbjct: 781 WPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAAL 840

Query: 841 ITLRQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDY 900
           ++LR R K++CRS V I K+ERDVYAA ID+KVA+KIGPGH+EPP+ S  WS+ +EG DY
Sbjct: 841 LSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDY 887

Query: 901 KVWEVS 904
           KVWE S
Sbjct: 901 KVWETS 887

BLAST of Carg17249 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 415.6 bits (1067), Expect = 1.0e-115
Identity = 190/395 (48.10%), Postives = 266/395 (67.34%), Query Frame = 0

Query: 510 GFEILCQGFNWESHKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 569
           G E++ Q +NWESHK   W+  L  K  +++  GFT  WLPPP++S++PEGY+P+DLY+L
Sbjct: 24  GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83

Query: 570 NSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-RLNWDDRAIV 629
           NS YG+   LK +++      ++ + D+V+NHR    +   G++N + G  L WD+ A+ 
Sbjct: 84  NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143

Query: 630 SDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 689
           S      G GN+S+GDNF+  PN+DH+Q FVR DI  WL WLR  +G+  +R DF RG+ 
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203

Query: 690 GGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 749
             YVK+Y+ A++P F+VGE WDS +Y    +D+NQD+HRQRI+ WI+AT   + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263

Query: 750 KGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 809
           KGIL  A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323

Query: 810 YAYILTHPGTPSVFFDHIL---SHYKSEIAALITLRQRNKVNCRSAVKIQKAERDVYAAT 869
           YAYILTHPG P VF+DH     S    +I  LI +R+R  ++ RS V++ KAE ++YAA 
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383

Query: 870 IDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 901
           + +K+ +K+G G + P      W+L   G+ Y VW
Sbjct: 384 VGEKICMKLGDGSWCPS--GRDWTLATSGHRYAVW 411

BLAST of Carg17249 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 356.7 bits (914), Expect = 5.6e-98
Identity = 180/399 (45.11%), Postives = 248/399 (62.16%), Query Frame = 0

Query: 513 ILCQGFNWES-HKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN- 572
           +L Q FNWES  K G +Y  L     ++++ G T LWLPPP++SV+PEGY+P  LY+LN 
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 573 SRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-----RLNWDDR 632
           S+YG+  ELK ++K  +  GIK L D+V+NHR A+ ++    +  F G     RL+WD  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 633 AIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVR 692
            +  +DP F G GN  +G +F  AP+IDH    V+ ++ EW+ WL+ EIG+ GWR D+VR
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 693 GFWGGYVKDYLDASEPYFAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWI-NATNGTAGA 752
           G+     K Y+  + P FAVGE WD + Y   G++D++Q+ HR  +  WI  A  G   A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 753 FDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGG 812
           FD TTKGIL SA+ + E WRL D +GKPPG++G  P  AVTFI+NHDT  T   W FP  
Sbjct: 267 FDFTTKGILQSAV-KGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSD 326

Query: 813 KEMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSAVKIQKAERDVY 872
           K + GY YILTHPGTP +F++H +    K  I+ L+ +R +N +   S+V I+ AE D+Y
Sbjct: 327 KVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLY 386

Query: 873 AATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
            A IDDKV +KIGP       V   ++L   G D+ VWE
Sbjct: 387 LAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421

BLAST of Carg17249 vs. TAIR 10
Match: AT1G10760.1 (Pyruvate phosphate dikinase, PEP/pyruvate binding domain )

HSP 1 Score: 48.5 bits (114), Expect = 3.2e-05
Identity = 31/91 (34.07%), Postives = 48/91 (52.75%), Query Frame = 0

Query: 124 LHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKIDLVP 183
           LHWG  L  D+   W  P +   P  +   K+ A+ TP  KS     G++  +++ID   
Sbjct: 120 LHWGAIL--DNKENWVLPSRS--PDRTQNFKNSALRTPFVKSG----GNSHLKLEID--- 179

Query: 184 DKAIAAINFVLKDEETGTWYQHKGRDFKVPL 215
           D AI AI F++ DE    WY++ G++F + L
Sbjct: 180 DPAIHAIEFLIFDESRNKWYKNNGQNFHINL 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013163.10.0e+00100.00Alpha-amylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022945374.10.0e+0099.67alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita moschata][more]
KAG6574106.10.0e+0099.67Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022967951.10.0e+0099.23alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita maxima][more]
XP_023542260.10.0e+0099.34alpha-amylase 3, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q94A410.0e+0066.67Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1[more]
Q8LFG11.4e-11448.10Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1[more]
P006932.5e-11147.56Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1[more]
P178592.5e-11149.75Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1[more]
P279347.3e-11148.50Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1G0U20.0e+0099.671,4-alpha-D-glucan glucanohydrolase OS=Cucurbita moschata OX=3662 GN=LOC11144962... [more]
A0A6J1HTI20.0e+0099.231,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467335 ... [more]
A0A6J1G0S50.0e+0098.01Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1[more]
A0A6J1G0P20.0e+0098.16Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1[more]
A0A6J1HS700.0e+0097.82Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111467335 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G69830.10.0e+0066.67alpha-amylase-like 3 [more]
AT1G76130.11.0e-11548.10alpha-amylase-like 2 [more]
AT4G25000.15.6e-9845.11alpha-amylase-like [more]
AT1G10760.13.2e-0534.07Pyruvate phosphate dikinase, PEP/pyruvate binding domain [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 454..474
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 512..845
e-value: 2.9E-126
score: 423.6
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 82..903
NoneNo IPR availablePANTHERPTHR43447:SF33ALPHA-AMYLASE 3, CHLOROPLASTICcoord: 82..903
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 513..852
e-value: 7.39336E-171
score: 495.973
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 846..901
IPR006046Alpha amylasePRINTSPR00110ALPHAAMYLASEcoord: 590..601
score: 48.94
coord: 780..792
score: 46.76
coord: 676..687
score: 32.03
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 512..841
e-value: 2.1E-41
score: 153.5
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 539..792
e-value: 7.9E-17
score: 61.8
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 842..902
e-value: 1.1E-27
score: 108.0
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 843..901
e-value: 2.3E-21
score: 75.8
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 846..902
e-value: 3.3E-20
score: 73.9
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 513..828

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg17249-RACarg17249-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030570 pectate lyase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0043169 cation binding