Homology
BLAST of Carg17249 vs. NCBI nr
Match:
KAG7013163.1 (Alpha-amylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1885.2 bits (4882), Expect = 0.0e+00
Identity = 904/904 (100.00%), Postives = 904/904 (100.00%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
Query: 901 EVSK 905
EVSK
Sbjct: 901 EVSK 904
BLAST of Carg17249 vs. NCBI nr
Match:
XP_022945374.1 (alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 1879.0 bits (4866), Expect = 0.0e+00
Identity = 901/904 (99.67%), Postives = 901/904 (99.67%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
Query: 901 EVSK 905
EVSK
Sbjct: 901 EVSK 904
BLAST of Carg17249 vs. NCBI nr
Match:
KAG6574106.1 (Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1879.0 bits (4866), Expect = 0.0e+00
Identity = 901/904 (99.67%), Postives = 901/904 (99.67%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 128 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 187
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 188 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 247
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 248 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 307
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 308 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 367
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 368 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 427
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 428 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 487
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 488 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 547
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 548 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 607
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 608 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 667
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 668 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 727
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 728 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 787
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 788 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 847
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 848 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 907
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 908 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 967
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 968 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 1027
Query: 901 EVSK 905
EVSK
Sbjct: 1028 EVSK 1031
BLAST of Carg17249 vs. NCBI nr
Match:
XP_022967951.1 (alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 1875.5 bits (4857), Expect = 0.0e+00
Identity = 897/904 (99.23%), Postives = 901/904 (99.67%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFF+KRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWY+ELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPS+SGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSISGGWSLVIEGNDYKVW 900
Query: 901 EVSK 905
EVSK
Sbjct: 901 EVSK 904
BLAST of Carg17249 vs. NCBI nr
Match:
XP_023542260.1 (alpha-amylase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1871.3 bits (4846), Expect = 0.0e+00
Identity = 898/904 (99.34%), Postives = 899/904 (99.45%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQR PKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRCPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCRLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIP GSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPSGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
Query: 901 EVSK 905
EVSK
Sbjct: 901 EVSK 904
BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match:
Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)
HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 604/906 (66.67%), Postives = 720/906 (79.47%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCG-KGKQRFQR-RPKFNPLKSCSSFTYCTNKLLYR-GRRTFFNRNSCT 60
MST+ ++ LL+H + + R F P+ + +NKLL+ G+ + + +
Sbjct: 1 MSTVPIESLLHHSYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVSSMNKS 60
Query: 61 LPVLRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCN 120
+RA+S++ ++E +S DV+ E F V+R EK EGKI V L + K +N WEL++GC+
Sbjct: 61 PVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WELSVGCS 120
Query: 121 LSGKWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEV 180
+ GKWILHWGVS + D+GSEWDQPP++M PPGSI IKDYAIETPLKK S GD+ EV
Sbjct: 121 IPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEV 180
Query: 181 KIDLVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGA 240
I+L + ++AA+NFVLKDEETG WYQHKGRDFKVPL+ +GN++G KKG GA
Sbjct: 181 AINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF----GA 240
Query: 241 LGQLSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCP 300
LGQLSN+ +K + +SS E+ +E+ L+ FYEE+PI K VA DNSVSVT RKCP
Sbjct: 241 LGQLSNIPLKQD------KSSAETDSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCP 300
Query: 301 ETTKYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDG 360
ET+K I+ +ETD+PGDVTVHWG C++ T+KWEIP+ P+P ET++FK KALRT LQ K DG
Sbjct: 301 ETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDG 360
Query: 361 KGSCGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVS 420
GS G+F+++ + G FVLK NEN+WLN +GEDFY+PF +S + PV+ E VS
Sbjct: 361 NGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSSSPVE-----TEAAQVS 420
Query: 421 IKSGEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYS 480
+ + VS + +T II EIRNL DISS K K KE QE+ILQEIEKLAAEAYS
Sbjct: 421 KPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 480
Query: 481 IFRSSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAA 540
IFRS+TP F+EE + + +P ++ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA
Sbjct: 481 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 540
Query: 541 ELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDV 600
EL+SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG IDELKD VK FH VGIKVLGD
Sbjct: 541 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDA 600
Query: 601 VLNHRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQD 660
VLNHRCA F+NQNG+WN+FGGRLNWDDRA+V+DDPHFQGRGNKSSGDNFHAAPNIDHSQD
Sbjct: 601 VLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQD 660
Query: 661 FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYG 720
FVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+PYFAVGEYWDSLSYTYG
Sbjct: 661 FVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG 720
Query: 721 EMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGW 780
EMD+NQDAHRQRIVDWINAT+G AGAFDVTTKGILH+ALQ+CEYWRLSD KGKPPGVVGW
Sbjct: 721 EMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGW 780
Query: 781 WPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAAL 840
WPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VFFDHI S Y SEIAAL
Sbjct: 781 WPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAAL 840
Query: 841 ITLRQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDY 900
++LR R K++CRS V I K+ERDVYAA ID+KVA+KIGPGH+EPP+ S WS+ +EG DY
Sbjct: 841 LSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDY 887
Query: 901 KVWEVS 904
KVWE S
Sbjct: 901 KVWETS 887
BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match:
Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)
HSP 1 Score: 415.6 bits (1067), Expect = 1.4e-114
Identity = 190/395 (48.10%), Postives = 266/395 (67.34%), Query Frame = 0
Query: 510 GFEILCQGFNWESHKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 569
G E++ Q +NWESHK W+ L K +++ GFT WLPPP++S++PEGY+P+DLY+L
Sbjct: 24 GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83
Query: 570 NSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-RLNWDDRAIV 629
NS YG+ LK +++ ++ + D+V+NHR + G++N + G L WD+ A+
Sbjct: 84 NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143
Query: 630 SDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 689
S G GN+S+GDNF+ PN+DH+Q FVR DI WL WLR +G+ +R DF RG+
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203
Query: 690 GGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 749
YVK+Y+ A++P F+VGE WDS +Y +D+NQD+HRQRI+ WI+AT + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263
Query: 750 KGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 809
KGIL A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323
Query: 810 YAYILTHPGTPSVFFDHIL---SHYKSEIAALITLRQRNKVNCRSAVKIQKAERDVYAAT 869
YAYILTHPG P VF+DH S +I LI +R+R ++ RS V++ KAE ++YAA
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383
Query: 870 IDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 901
+ +K+ +K+G G + P W+L G+ Y VW
Sbjct: 384 VGEKICMKLGDGSWCPS--GRDWTLATSGHRYAVW 411
BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match:
P00693 (Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1)
HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-111
Identity = 195/410 (47.56%), Postives = 269/410 (65.61%), Query Frame = 0
Query: 505 SGTGSGFEILCQGFNWESHK-SGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMP 564
+G SG ++L QGFNWES K SG WY + K ++++ G T +WLPPP+ SVS EGYMP
Sbjct: 19 AGLASGHQVLFQGFNWESWKQSGGWYNMMMGKVDDIAAAGVTHVWLPPPSHSVSNEGYMP 78
Query: 565 KDLYNLN-SRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIF-----G 624
LY+++ S+YGN ELK ++ H G++ + D+V+NHRCA +++ GI+ IF
Sbjct: 79 GRLYDIDASKYGNAAELKSLIGALHGKGVQAIADIVINHRCADYKDSRGIYCIFEGGTSD 138
Query: 625 GRLNWDDRAIVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGY 684
GRL+W I DD + G N +G +F AAP+IDH D V+ ++KEWL WL+ ++G+
Sbjct: 139 GRLDWGPHMICRDDTKYSDGTANLDTGADFAAAPDIDHLNDRVQRELKEWLLWLKSDLGF 198
Query: 685 DGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWIN 744
D WRLDF RG+ K Y+D + P AV E WD+++ G+ +++QDAHRQ +V+W++
Sbjct: 199 DAWRLDFARGYSPEMAKVYIDGTSPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVD 258
Query: 745 ATNGTAGA---FDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTG 804
G A A FD TTKGIL++A++ E WRL D +GK PGV+GWWP++A TF++NHDTG
Sbjct: 259 KVGGAASAGMVFDFTTKGILNAAVEG-ELWRLIDPQGKAPGVMGWWPAKAATFVDNHDTG 318
Query: 805 STQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSA 864
STQ W FP K MQGYAYILTHPG P +F+DH + +K +IAAL+ +R+RN + SA
Sbjct: 319 STQAMWPFPSDKVMQGYAYILTHPGIPCIFYDHFFNWGFKDQIAALVAIRKRNGITATSA 378
Query: 865 VKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
+KI E D Y A ID KV VKIG + + G+ GNDY VWE
Sbjct: 379 LKILMHEGDAYVAEIDGKVVVKIGSRYDVGAVIPAGFVTSAHGNDYAVWE 427
BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match:
P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)
HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-111
Identity = 198/398 (49.75%), Postives = 264/398 (66.33%), Query Frame = 0
Query: 513 ILCQGFNWESHKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN-S 572
+L QGFNWES K G WY LK +L++ G T +WLPPP++SVSPEGY+P LY+L+ S
Sbjct: 25 LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84
Query: 573 RYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-----RLNWDDRA 632
+YG+ +ELK ++ FH+ GIK L D+V+NHR A+ ++ GI+ IF G R +W
Sbjct: 85 KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144
Query: 633 IVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVR 692
I DD + G GN SG+ + AAP+IDH V+ ++ EW+ WL+ EIG+DGWR DFV+
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204
Query: 693 GFWGGYVKDYLDASEPYFAVGEYWDSLSYTY-GEMDHNQDAHRQRIVDWINATNGTAGAF 752
G+ K Y++ ++P FAVGE WDS+SY G+ ++NQD+HR +V+W+ + G AF
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAF 264
Query: 753 DVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGK 812
D TTKGIL +A+Q E WRL D GKPPG++G P AVTFI+NHDTGSTQ W FP K
Sbjct: 265 DFTTKGILQAAVQG-ELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324
Query: 813 EMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSAVKIQKAERDVYA 872
MQGYAYILTHPGTPS+F+DH K +IA L ++R RN +N +S VKI +E D+Y
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYV 384
Query: 873 ATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
A ID+K+ VKIGP + + G DY VWE
Sbjct: 385 AKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421
BLAST of Carg17249 vs. ExPASy Swiss-Prot
Match:
P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)
HSP 1 Score: 403.3 bits (1035), Expect = 7.3e-111
Identity = 194/400 (48.50%), Postives = 261/400 (65.25%), Query Frame = 0
Query: 512 EILCQGFNWES-HKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 571
++L QGFNWES K G WY L EK E++S G T +WLPPP+ SVSP+GYMP LY+L+
Sbjct: 26 QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85
Query: 572 -SRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIF-----GGRLNWDD 631
S+YG ELK +++ FHD ++ L D+V+NHRCA +++ G++ +F GRL+W
Sbjct: 86 ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145
Query: 632 RAIVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDF 691
I SDD + GRG++ +G F AAP+IDH V+ ++ +WL WLR ++G+DGWRLDF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205
Query: 692 VRGFWGGYVKDYLDASEPYFAVGEYWDSLSYT-YGEMDHNQDAHRQRIVDWINATNGTAG 751
+G+ + Y+D + P F VGE W SL Y G+ NQDA RQ +V+W+ A
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265
Query: 752 AFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPG 811
AFD TTKGIL +A+Q E WRL D GK PG++GW P +AVTF++NHDTGSTQ W FP
Sbjct: 266 AFDFTTKGILQAAVQG-ELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPS 325
Query: 812 GKEMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSAVKIQKAERDV 871
K MQGYAYILTHPG P +F+DH+ + + EIA L +R RN ++ S + I KAE D+
Sbjct: 326 DKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDI 385
Query: 872 YAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
Y A ID KV K+GP + + + +V GNDY VWE
Sbjct: 386 YVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424
BLAST of Carg17249 vs. ExPASy TrEMBL
Match:
A0A6J1G0U2 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1)
HSP 1 Score: 1879.0 bits (4866), Expect = 0.0e+00
Identity = 901/904 (99.67%), Postives = 901/904 (99.67%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
Query: 901 EVSK 905
EVSK
Sbjct: 901 EVSK 904
BLAST of Carg17249 vs. ExPASy TrEMBL
Match:
A0A6J1HTI2 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467335 PE=3 SV=1)
HSP 1 Score: 1875.5 bits (4857), Expect = 0.0e+00
Identity = 897/904 (99.23%), Postives = 901/904 (99.67%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFF+KRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWY+ELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPS+SGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSISGGWSLVIEGNDYKVW 900
Query: 901 EVSK 905
EVSK
Sbjct: 901 EVSK 904
BLAST of Carg17249 vs. ExPASy TrEMBL
Match:
A0A6J1G0S5 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1)
HSP 1 Score: 1841.6 bits (4769), Expect = 0.0e+00
Identity = 886/904 (98.01%), Postives = 886/904 (98.01%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVS VTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVS---------------VTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 900
RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW
Sbjct: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 889
Query: 901 EVSK 905
EVSK
Sbjct: 901 EVSK 889
BLAST of Carg17249 vs. ExPASy TrEMBL
Match:
A0A6J1G0P2 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1)
HSP 1 Score: 1774.2 bits (4594), Expect = 0.0e+00
Identity = 854/870 (98.16%), Postives = 855/870 (98.28%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSN IVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNFIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS
Sbjct: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSS NMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSSNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKV 871
RQRNKVNCRSAV I D+ I KV
Sbjct: 841 RQRNKVNCRSAVSI----HDLLCVLITSKV 866
BLAST of Carg17249 vs. ExPASy TrEMBL
Match:
A0A6J1HS70 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111467335 PE=3 SV=1)
HSP 1 Score: 1771.1 bits (4586), Expect = 0.0e+00
Identity = 851/870 (97.82%), Postives = 855/870 (98.28%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCTLPV 60
MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSC LPV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60
Query: 61 LRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
LRASSTNAGLLEPFESTDVLLTETFF+KRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPETT 300
Query: 301 KYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDGKGS 360
KY+LYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQ KKDGKGS
Sbjct: 301 KYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKGS 360
Query: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVSIKS 420
CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLED GVSIKS
Sbjct: 361 CGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIKS 420
Query: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR
Sbjct: 421 GEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAAELS 540
SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWY+ELKEKAAELS
Sbjct: 481 SSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALITL 840
Query: 841 RQRNKVNCRSAVKIQKAERDVYAATIDDKV 871
RQRNKVNCRSAV I D+ I KV
Sbjct: 841 RQRNKVNCRSAVSI----HDLLCVLITSKV 866
BLAST of Carg17249 vs. TAIR 10
Match:
AT1G69830.1 (alpha-amylase-like 3 )
HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 604/906 (66.67%), Postives = 720/906 (79.47%), Query Frame = 0
Query: 1 MSTIVLDPLLYHCG-KGKQRFQR-RPKFNPLKSCSSFTYCTNKLLYR-GRRTFFNRNSCT 60
MST+ ++ LL+H + + R F P+ + +NKLL+ G+ + + +
Sbjct: 1 MSTVPIESLLHHSYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVSSMNKS 60
Query: 61 LPVLRASSTNAGLLEPFESTDVLLTETFFVKRTEKLEGKISVGLAQGKNQNQWELTIGCN 120
+RA+S++ ++E +S DV+ E F V+R EK EGKI V L + K +N WEL++GC+
Sbjct: 61 PVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WELSVGCS 120
Query: 121 LSGKWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEV 180
+ GKWILHWGVS + D+GSEWDQPP++M PPGSI IKDYAIETPLKK S GD+ EV
Sbjct: 121 IPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEV 180
Query: 181 KIDLVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGA 240
I+L + ++AA+NFVLKDEETG WYQHKGRDFKVPL+ +GN++G KKG GA
Sbjct: 181 AINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF----GA 240
Query: 241 LGQLSNLIVKPEPNSKGQESSGESGDTKEKNYLEGFYEELPIVKEVAVDNSVSVTVRKCP 300
LGQLSN+ +K + +SS E+ +E+ L+ FYEE+PI K VA DNSVSVT RKCP
Sbjct: 241 LGQLSNIPLKQD------KSSAETDSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCP 300
Query: 301 ETTKYILYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQLKKDG 360
ET+K I+ +ETD+PGDVTVHWG C++ T+KWEIP+ P+P ET++FK KALRT LQ K DG
Sbjct: 301 ETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDG 360
Query: 361 KGSCGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDRGVS 420
GS G+F+++ + G FVLK NEN+WLN +GEDFY+PF +S + PV+ E VS
Sbjct: 361 NGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSSSPVE-----TEAAQVS 420
Query: 421 IKSGEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYS 480
+ + VS + +T II EIRNL DISS K K KE QE+ILQEIEKLAAEAYS
Sbjct: 421 KPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 480
Query: 481 IFRSSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYVELKEKAA 540
IFRS+TP F+EE + + +P ++ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA
Sbjct: 481 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 540
Query: 541 ELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDV 600
EL+SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG IDELKD VK FH VGIKVLGD
Sbjct: 541 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDA 600
Query: 601 VLNHRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQD 660
VLNHRCA F+NQNG+WN+FGGRLNWDDRA+V+DDPHFQGRGNKSSGDNFHAAPNIDHSQD
Sbjct: 601 VLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQD 660
Query: 661 FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYG 720
FVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+PYFAVGEYWDSLSYTYG
Sbjct: 661 FVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG 720
Query: 721 EMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGW 780
EMD+NQDAHRQRIVDWINAT+G AGAFDVTTKGILH+ALQ+CEYWRLSD KGKPPGVVGW
Sbjct: 721 EMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGW 780
Query: 781 WPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAAL 840
WPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VFFDHI S Y SEIAAL
Sbjct: 781 WPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAAL 840
Query: 841 ITLRQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDY 900
++LR R K++CRS V I K+ERDVYAA ID+KVA+KIGPGH+EPP+ S WS+ +EG DY
Sbjct: 841 LSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDY 887
Query: 901 KVWEVS 904
KVWE S
Sbjct: 901 KVWETS 887
BLAST of Carg17249 vs. TAIR 10
Match:
AT1G76130.1 (alpha-amylase-like 2 )
HSP 1 Score: 415.6 bits (1067), Expect = 1.0e-115
Identity = 190/395 (48.10%), Postives = 266/395 (67.34%), Query Frame = 0
Query: 510 GFEILCQGFNWESHKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 569
G E++ Q +NWESHK W+ L K +++ GFT WLPPP++S++PEGY+P+DLY+L
Sbjct: 24 GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83
Query: 570 NSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-RLNWDDRAIV 629
NS YG+ LK +++ ++ + D+V+NHR + G++N + G L WD+ A+
Sbjct: 84 NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143
Query: 630 SDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 689
S G GN+S+GDNF+ PN+DH+Q FVR DI WL WLR +G+ +R DF RG+
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203
Query: 690 GGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 749
YVK+Y+ A++P F+VGE WDS +Y +D+NQD+HRQRI+ WI+AT + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263
Query: 750 KGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 809
KGIL A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323
Query: 810 YAYILTHPGTPSVFFDHIL---SHYKSEIAALITLRQRNKVNCRSAVKIQKAERDVYAAT 869
YAYILTHPG P VF+DH S +I LI +R+R ++ RS V++ KAE ++YAA
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383
Query: 870 IDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVW 901
+ +K+ +K+G G + P W+L G+ Y VW
Sbjct: 384 VGEKICMKLGDGSWCPS--GRDWTLATSGHRYAVW 411
BLAST of Carg17249 vs. TAIR 10
Match:
AT4G25000.1 (alpha-amylase-like )
HSP 1 Score: 356.7 bits (914), Expect = 5.6e-98
Identity = 180/399 (45.11%), Postives = 248/399 (62.16%), Query Frame = 0
Query: 513 ILCQGFNWES-HKSGRWYVELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN- 572
+L Q FNWES K G +Y L ++++ G T LWLPPP++SV+PEGY+P LY+LN
Sbjct: 27 LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86
Query: 573 SRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAQFQNQNGIWNIFGG-----RLNWDDR 632
S+YG+ ELK ++K + GIK L D+V+NHR A+ ++ + F G RL+WD
Sbjct: 87 SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146
Query: 633 AIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVR 692
+ +DP F G GN +G +F AP+IDH V+ ++ EW+ WL+ EIG+ GWR D+VR
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206
Query: 693 GFWGGYVKDYLDASEPYFAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWI-NATNGTAGA 752
G+ K Y+ + P FAVGE WD + Y G++D++Q+ HR + WI A G A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266
Query: 753 FDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGG 812
FD TTKGIL SA+ + E WRL D +GKPPG++G P AVTFI+NHDT T W FP
Sbjct: 267 FDFTTKGILQSAV-KGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSD 326
Query: 813 KEMQGYAYILTHPGTPSVFFDHILS-HYKSEIAALITLRQRNKVNCRSAVKIQKAERDVY 872
K + GY YILTHPGTP +F++H + K I+ L+ +R +N + S+V I+ AE D+Y
Sbjct: 327 KVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLY 386
Query: 873 AATIDDKVAVKIGPGHFEPPSVSGGWSLVIEGNDYKVWE 902
A IDDKV +KIGP V ++L G D+ VWE
Sbjct: 387 LAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421
BLAST of Carg17249 vs. TAIR 10
Match:
AT1G10760.1 (Pyruvate phosphate dikinase, PEP/pyruvate binding domain )
HSP 1 Score: 48.5 bits (114), Expect = 3.2e-05
Identity = 31/91 (34.07%), Postives = 48/91 (52.75%), Query Frame = 0
Query: 124 LHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKIDLVP 183
LHWG L D+ W P + P + K+ A+ TP KS G++ +++ID
Sbjct: 120 LHWGAIL--DNKENWVLPSRS--PDRTQNFKNSALRTPFVKSG----GNSHLKLEID--- 179
Query: 184 DKAIAAINFVLKDEETGTWYQHKGRDFKVPL 215
D AI AI F++ DE WY++ G++F + L
Sbjct: 180 DPAIHAIEFLIFDESRNKWYKNNGQNFHINL 199
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7013163.1 | 0.0e+00 | 100.00 | Alpha-amylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022945374.1 | 0.0e+00 | 99.67 | alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita moschata] | [more] |
KAG6574106.1 | 0.0e+00 | 99.67 | Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_022967951.1 | 0.0e+00 | 99.23 | alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita maxima] | [more] |
XP_023542260.1 | 0.0e+00 | 99.34 | alpha-amylase 3, chloroplastic-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q94A41 | 0.0e+00 | 66.67 | Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1 | [more] |
Q8LFG1 | 1.4e-114 | 48.10 | Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1 | [more] |
P00693 | 2.5e-111 | 47.56 | Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1 | [more] |
P17859 | 2.5e-111 | 49.75 | Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1 | [more] |
P27934 | 7.3e-111 | 48.50 | Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1G0U2 | 0.0e+00 | 99.67 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita moschata OX=3662 GN=LOC11144962... | [more] |
A0A6J1HTI2 | 0.0e+00 | 99.23 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467335 ... | [more] |
A0A6J1G0S5 | 0.0e+00 | 98.01 | Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1 | [more] |
A0A6J1G0P2 | 0.0e+00 | 98.16 | Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111449629 PE=3 SV=1 | [more] |
A0A6J1HS70 | 0.0e+00 | 97.82 | Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111467335 PE=3 SV=1 | [more] |