Carg17233 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17233
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	dnaJ homolog subfamily B member 1-like
Location	Carg_Chr18: 11019244 .. 11020418 (-)
RNA-Seq Expression	Carg17233
Synteny	Carg17233

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGAATGCTAATAAATCTAATTTGTTTCGATTGTATCTAATGGTATCTTTGATGATGCAAGGCTTAATGGACGGAAATGAAGGAGAATATTGCGAAGATATTATTCTTTTTTTCTAGCTGCCTGCCTAGGATTATCAGGATATTGATACCTCTTTAAATGGCGAACGATCCCTTCTATTATACTCTTACCATTGATGTAAAGACGATAGGATAAACTAATATGAGCCAGTGGTTTGCAACTTTGAATATTATATAGCATAGCATGATATGTCAAATTCTTGTCTGCCTAAAGTAAAGACATACTTGTGTAGCTAATTGGATGATTCTGCGTGCTTTTCAGAAAGACAGATGATCATATGAATATCATGCTCTGACATAAACTTTATGATCAGAAAGGGAACTTGGTGCTTCTTCTTTCTTCAATGGCCACAGGACGCATCTATAACGATATTTTCTGTTGATTATGAATAATTAACATGCTTTTTCTTGATGAAAATTGGAGAAGAACATCATTGAATCGAATTAAATGAACATAAGCTTACTCTTCCTTTTTGGCATAATTGCAATCAAGCAAACACTTAACTTCATTGTGTGATGATTCGTCTAATACATTGGGTTTGTGTGACACCCTATTTCATTCTCTCTTTTCATATCTTCATTGTTCTTGCATAGTTTTCGTCTCGTCTTTCTCCTCGATTTAGGCTTGACATTCAAGTAACTTGTTTTGCAGGAAGCCGAAAACTGTTGATGAAGTGTTAAAAATCGATATCAAGCCTGGTTGGAAGAAGGGGACAAAAATCACTTTCCCTGAGAAAGGCAATCAAGAGCCTGGGGTCGCCCCTGCTGATCTTATATTTATCATCGATGAGAAACCGCATCCTGTGTTTGAAAGGGATGGTAATGATTTGGTAGTGAATCAGAAACTATCTCTACTAGAAGCACTAACCGGAAAGACATTGAACTTTACAACTTTGGATGGAAGAAATCTCACGACTGTTACTGATATTGTCAAACCGGGTTACGAGGTAGTGATCCAGAATGAAGGAATGCCCATCTCTAAAGAACCAAACAAGAAGGGGAACCTTAGAATCAAGTTTGATATAGTTTTCCCATCAAAGCTTACATTTGATCAGAAATCCGATCTAAGAAGGGCTTTGGGTGGTTCTGATTAA

mRNA sequence

ATGTTGAATGCTAATAAATCTAATTTGTTTCGATTGAAGCCGAAAACTGTTGATGAAGTGTTAAAAATCGATATCAAGCCTGGTTGGAAGAAGGGGACAAAAATCACTTTCCCTGAGAAAGGCAATCAAGAGCCTGGGGTCGCCCCTGCTGATCTTATATTTATCATCGATGAGAAACCGCATCCTGTGTTTGAAAGGGATGGTAATGATTTGGTAGTGAATCAGAAACTATCTCTACTAGAAGCACTAACCGGAAAGACATTGAACTTTACAACTTTGGATGGAAGAAATCTCACGACTGTTACTGATATTGTCAAACCGGGTTACGAGGTAGTGATCCAGAATGAAGGAATGCCCATCTCTAAAGAACCAAACAAGAAGGGGAACCTTAGAATCAAGTTTGATATAGTTTTCCCATCAAAGCTTACATTTGATCAGAAATCCGATCTAAGAAGGGCTTTGGGTGGTTCTGATTAA

Coding sequence (CDS)

ATGTTGAATGCTAATAAATCTAATTTGTTTCGATTGAAGCCGAAAACTGTTGATGAAGTGTTAAAAATCGATATCAAGCCTGGTTGGAAGAAGGGGACAAAAATCACTTTCCCTGAGAAAGGCAATCAAGAGCCTGGGGTCGCCCCTGCTGATCTTATATTTATCATCGATGAGAAACCGCATCCTGTGTTTGAAAGGGATGGTAATGATTTGGTAGTGAATCAGAAACTATCTCTACTAGAAGCACTAACCGGAAAGACATTGAACTTTACAACTTTGGATGGAAGAAATCTCACGACTGTTACTGATATTGTCAAACCGGGTTACGAGGTAGTGATCCAGAATGAAGGAATGCCCATCTCTAAAGAACCAAACAAGAAGGGGAACCTTAGAATCAAGTTTGATATAGTTTTCCCATCAAAGCTTACATTTGATCAGAAATCCGATCTAAGAAGGGCTTTGGGTGGTTCTGATTAA

Protein sequence

MLNANKSNLFRLKPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIVFPSKLTFDQKSDLRRALGGSD

Homology

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BLAST of Carg17233 vs. NCBI nr
Match: KAG7013179.1 (DnaJ-like subfamily B member 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 319.7 bits (818), Expect = 1.4e-83
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MLNANKSNLFRLKPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKP 60
           MLNANKSNLFRLKPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKP
Sbjct: 1   MLNANKSNLFRLKPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKP 60

Query: 61  HPVFERDGNDLVVNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPI 120
           HPVFERDGNDLVVNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPI
Sbjct: 61  HPVFERDGNDLVVNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPI 120

Query: 121 SKEPNKKGNLRIKFDIVFPSKLTFDQKSDLRRALGGSD 159
           SKEPNKKGNLRIKFDIVFPSKLTFDQKSDLRRALGGSD
Sbjct: 121 SKEPNKKGNLRIKFDIVFPSKLTFDQKSDLRRALGGSD 158

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BLAST of Carg17233 vs. NCBI nr
Match: XP_022945456.1 (dnaJ homolog subfamily B member 1-like [Cucurbita moschata])

HSP 1 Score: 297.0 bits (759), Expect = 9.8e-77
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTFDQKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFDQKSDLRRALGGSD 315

[Close]

BLAST of Carg17233 vs. NCBI nr
Match: XP_022968262.1 (dnaJ homolog subfamily B member 1-like [Cucurbita maxima])

HSP 1 Score: 297.0 bits (759), Expect = 9.8e-77
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTFDQKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFDQKSDLRRALGGSD 315

[Close]

BLAST of Carg17233 vs. NCBI nr
Match: XP_023541690.1 (dnaJ homolog subfamily B member 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.0 bits (754), Expect = 3.7e-76
Identity = 145/146 (99.32%), Postives = 145/146 (99.32%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQKLSLLEALTG TLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKLSLLEALTGNTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTFDQKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFDQKSDLRRALGGSD 315

[Close]

BLAST of Carg17233 vs. NCBI nr
Match: XP_008446295.1 (PREDICTED: dnaJ homolog subfamily B member 1 [Cucumis melo] >KAA0034375.1 dnaJ-like protein subfamily B member 1 [Cucumis melo var. makuwa] >TYK15544.1 dnaJ-like protein subfamily B member 1 [Cucumis melo var. makuwa])

HSP 1 Score: 288.9 bits (738), Expect = 2.7e-74
Identity = 141/146 (96.58%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQK+SLLEALTGKTLN TTLDGR+LTTVTDIVKPGYEVV QNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKMSLLEALTGKTLNITTLDGRDLTTVTDIVKPGYEVVFQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTF+QKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFEQKSDLRRALGGSD 315

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BLAST of Carg17233 vs. ExPASy Swiss-Prot
Match: Q9D832 (DnaJ homolog subfamily B member 4 OS=Mus musculus OX=10090 GN=Dnajb4 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.1e-33
Identity = 69/144 (47.92%), Postives = 101/144 (70.14%), Query Frame = 0


Query: 15  KTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVN 74
           ++ D++L I+IK GWK+GTKITFP +G++ P   PAD++F+I +K HP F+RDG+++V  
Sbjct: 194 RSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFVIKDKEHPKFKRDGSNIVYT 253

Query: 75  QKLSLLEALTGKTLNFTTLDGRNL-TTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIK 134
            K+SL EAL G +LN  T+DGRNL  +VTDIVKPG    +   G+P  K P+++G+L I+
Sbjct: 254 AKISLREALCGCSLNVPTMDGRNLPMSVTDIVKPGMRRRVIGYGLPFPKNPDQRGDLLIE 313

Query: 135 FDIVFPSKLTFDQKSDLRRALGGS 158
           FD+ FP  ++   K  LR+ L  S
Sbjct: 314 FDVSFPDVISAASKEILRKHLPAS 337

[Close]

BLAST of Carg17233 vs. ExPASy Swiss-Prot
Match: Q9UDY4 (DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 6.9e-33
Identity = 67/144 (46.53%), Postives = 100/144 (69.44%), Query Frame = 0


Query: 15  KTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVN 74
           ++ D++L I+IK GWK+GTKITFP +G++ P   PAD++FII +K HP F+RDG++++  
Sbjct: 194 RSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYT 253

Query: 75  QKLSLLEALTGKTLNFTTLDGRNL-TTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIK 134
            K+SL EAL G ++N  TLDGRN+  +V DIVKPG    I   G+P  K P+++G+L I+
Sbjct: 254 AKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIE 313

Query: 135 FDIVFPSKLTFDQKSDLRRALGGS 158
           F++ FP  ++   K  LR+ L  S
Sbjct: 314 FEVSFPDTISSSSKEVLRKHLPAS 337

[Close]

BLAST of Carg17233 vs. ExPASy Swiss-Prot
Match: Q5R8J8 (DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 6.9e-33
Identity = 67/144 (46.53%), Postives = 100/144 (69.44%), Query Frame = 0


Query: 15  KTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVN 74
           ++ D++L I+IK GWK+GTKITFP +G++ P   PAD++FII +K HP F+RDG++++  
Sbjct: 194 RSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYT 253

Query: 75  QKLSLLEALTGKTLNFTTLDGRNL-TTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIK 134
            K+SL EAL G ++N  TLDGRN+  +V DIVKPG    I   G+P  K P+++G+L I+
Sbjct: 254 AKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIE 313

Query: 135 FDIVFPSKLTFDQKSDLRRALGGS 158
           F++ FP  ++   K  LR+ L  S
Sbjct: 314 FEVSFPDTISSSSKEVLRKHLPAS 337

[Close]

BLAST of Carg17233 vs. ExPASy Swiss-Prot
Match: Q2KIT4 (DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.0e-32
Identity = 65/144 (45.14%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 15  KTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVN 74
           +T D++L I+IK GWK+GTKITFP +G++ P   PAD++F+I +K HP F+RDG++++  
Sbjct: 194 RTEDKILTIEIKKGWKEGTKITFPREGDETPTSIPADIVFVIKDKDHPKFKRDGSNIIYT 253

Query: 75  QKLSLLEALTGKTLNFTTLDGRNL-TTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIK 134
            K+SL EAL G ++N  T+DGR +  T+ DIVKPG    I   G+P  K P+++G+L I+
Sbjct: 254 AKISLREALCGCSINVPTMDGRTIPMTINDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIE 313

Query: 135 FDIVFPSKLTFDQKSDLRRALGGS 158
           F++ FP  ++   K  LR+ L  S
Sbjct: 314 FEVSFPDTISSSSKEVLRKHLPAS 337

[Close]

BLAST of Carg17233 vs. ExPASy Swiss-Prot
Match: Q9QYJ3 (DnaJ homolog subfamily B member 1 OS=Mus musculus OX=10090 GN=Dnajb1 PE=1 SV=3)

HSP 1 Score: 139.0 bits (349), Expect = 4.5e-32
Identity = 59/138 (42.75%), Postives = 98/138 (71.01%), Query Frame = 0


Query: 18  DEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVNQKL 77
           D++L I++K GWK+GTKITFP++G+Q     PAD++F++ +KPH +F+RDG+D++   ++
Sbjct: 201 DKILTIEVKRGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARI 260

Query: 78  SLLEALTGKTLNFTTLDGRNLTTV-TDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDI 137
           SL EAL G T+N  TLDGR +  V  D+++PG    +  EG+P+ K P K+G+L I+F++
Sbjct: 261 SLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLVIEFEV 320

Query: 138 VFPSKLTFDQKSDLRRAL 155
           +FP ++    ++ L + L
Sbjct: 321 IFPERIPVSSRTILEQVL 338

[Close]

BLAST of Carg17233 vs. ExPASy TrEMBL
Match: A0A6J1HT51 (dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467551 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.8e-77
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTFDQKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFDQKSDLRRALGGSD 315

[Close]

BLAST of Carg17233 vs. ExPASy TrEMBL
Match: A0A6J1G0W9 (dnaJ homolog subfamily B member 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449687 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.8e-77
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTFDQKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFDQKSDLRRALGGSD 315

[Close]

BLAST of Carg17233 vs. ExPASy TrEMBL
Match: A0A5A7SYT8 (DnaJ-like protein subfamily B member 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00360 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.3e-74
Identity = 141/146 (96.58%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQK+SLLEALTGKTLN TTLDGR+LTTVTDIVKPGYEVV QNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKMSLLEALTGKTLNITTLDGRDLTTVTDIVKPGYEVVFQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTF+QKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFEQKSDLRRALGGSD 315

[Close]

BLAST of Carg17233 vs. ExPASy TrEMBL
Match: A0A1S3BFJ0 (dnaJ homolog subfamily B member 1 OS=Cucumis melo OX=3656 GN=LOC103489072 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.3e-74
Identity = 141/146 (96.58%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 170 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 229

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQK+SLLEALTGKTLN TTLDGR+LTTVTDIVKPGYEVV QNEGMPISKEPNKKGNLRI
Sbjct: 230 VNQKMSLLEALTGKTLNITTLDGRDLTTVTDIVKPGYEVVFQNEGMPISKEPNKKGNLRI 289

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDIVFPSKLTF+QKSDLRRALGGSD
Sbjct: 290 KFDIVFPSKLTFEQKSDLRRALGGSD 315

[Close]

BLAST of Carg17233 vs. ExPASy TrEMBL
Match: A0A0A0KSI9 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G604140 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.9e-74
Identity = 140/146 (95.89%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV
Sbjct: 171 KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 230

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 132
           VNQK+SLLEALTGKTLN TTLDGR+L TVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI
Sbjct: 231 VNQKISLLEALTGKTLNITTLDGRDLPTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRI 290

Query: 133 KFDIVFPSKLTFDQKSDLRRALGGSD 159
           KFDI+FPSKLTF+QKSDLRRALGGSD
Sbjct: 291 KFDIIFPSKLTFEQKSDLRRALGGSD 316

[Close]

BLAST of Carg17233 vs. TAIR 10
Match: AT1G59725.1 (DNAJ heat shock family protein )

HSP 1 Score: 224.6 bits (571), Expect = 5.8e-59
Identity = 109/146 (74.66%), Postives = 129/146 (88.36%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           K K V+E+LKIDI PGWKKGTKITFPEKGNQEPGV PADLIF+IDEKPH V++RDGNDL+
Sbjct: 186 KSKPVEEILKIDITPGWKKGTKITFPEKGNQEPGVTPADLIFVIDEKPHSVYKRDGNDLI 245

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLT-TVTDIVKPGYEVVIQNEGMPISKEPNKKGNLR 132
           V++K+SLLEALTG TL+ TTLDGRNLT  V DIVKPG E+VI +EGMPISKE +K+G+LR
Sbjct: 246 VDKKVSLLEALTGITLSLTTLDGRNLTIPVLDIVKPGQEIVIPSEGMPISKEGSKRGDLR 305

Query: 133 IKFDIVFPSKLTFDQKSDLRRALGGS 158
           I F+I FPS+LT +QK+DL+R LGGS
Sbjct: 306 INFEICFPSRLTSEQKTDLKRVLGGS 331

[Close]

BLAST of Carg17233 vs. TAIR 10
Match: AT1G10350.1 (DNAJ heat shock family protein )

HSP 1 Score: 221.5 bits (563), Expect = 4.9e-58
Identity = 105/146 (71.92%), Postives = 130/146 (89.04%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           KPKTV E+LKIDIKPGWKKGTKITFPEKGNQEPGV PADLIF++DEKPH VF+RDGNDL+
Sbjct: 205 KPKTVQEILKIDIKPGWKKGTKITFPEKGNQEPGVTPADLIFVVDEKPHSVFKRDGNDLI 264

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLT-TVTDIVKPGYEVVIQNEGMPISKEPNKKGNLR 132
           + +K+SL++ALTG T++ TTLDGR+LT  V DIVKPG E+VI NEGMP +K+P K+G+LR
Sbjct: 265 LEKKVSLIDALTGLTISVTTLDGRSLTIPVLDIVKPGQEIVIPNEGMP-TKDPLKRGDLR 324

Query: 133 IKFDIVFPSKLTFDQKSDLRRALGGS 158
           + F+I+FPS+LT +QK+DL+R LGGS
Sbjct: 325 VTFEILFPSRLTSEQKNDLKRVLGGS 349

[Close]

BLAST of Carg17233 vs. TAIR 10
Match: AT3G47940.1 (DNAJ heat shock family protein )

HSP 1 Score: 206.8 bits (525), Expect = 1.2e-53
Identity = 90/139 (64.75%), Postives = 122/139 (87.77%), Query Frame = 0


Query: 19  EVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVNQKLS 78
           E+L I+IKPGWKKGTK+TFP+KGN+EPG+ PAD++F+++EKPHPV++RDGNDL+V+Q+++
Sbjct: 210 EILPIEIKPGWKKGTKLTFPKKGNEEPGIIPADIVFVVEEKPHPVYKRDGNDLLVSQEIT 269

Query: 79  LLEALTGKTLNFTTLDGRNL-TTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIV 138
           LLEALTGKT+N  TLDGR L   +T+I+KP +E+V+ NEGMPISKEP KKGNL++K  + 
Sbjct: 270 LLEALTGKTVNLITLDGRTLMIPLTEIIKPDHEIVVPNEGMPISKEPGKKGNLKLKLSVK 329

Query: 139 FPSKLTFDQKSDLRRALGG 157
           +PS+LT DQK +L+R LGG
Sbjct: 330 YPSRLTSDQKFELKRVLGG 348

[Close]

BLAST of Carg17233 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 204.5 bits (519), Expect = 6.2e-53
Identity = 95/144 (65.97%), Postives = 120/144 (83.33%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           K   V+E+L ID+KPGWKKGTKITFPEKGN++PGV PADL+FIIDEKPHPVF R+GNDL+
Sbjct: 194 KTMQVEEILTIDVKPGWKKGTKITFPEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLI 253

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLT-TVTDIVKPGYEVVIQNEGMPISKEPNKKGNLR 132
           V QK+SL+EALTG T+N TTLDGR LT  VT++V P YE V+  EGMP+ K+  K+GNLR
Sbjct: 254 VTQKISLVEALTGYTVNLTTLDGRRLTIPVTNVVHPEYEEVVPKEGMPLQKDQTKRGNLR 313

Query: 133 IKFDIVFPSKLTFDQKSDLRRALG 156
           IKF+I FP++LT +QK+ +++ LG
Sbjct: 314 IKFNIKFPTRLTSEQKTGVKKLLG 337

[Close]

BLAST of Carg17233 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 198.0 bits (502), Expect = 5.8e-51
Identity = 89/146 (60.96%), Postives = 119/146 (81.51%), Query Frame = 0


Query: 13  KPKTVDEVLKIDIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLV 72
           +P TV+E+L I+IKPGWKKGTKITFPEKGN++ G+ P+DL+FI+DEKPH VF+RDGNDLV
Sbjct: 178 RPTTVEEILTIEIKPGWKKGTKITFPEKGNEQRGIIPSDLVFIVDEKPHAVFKRDGNDLV 237

Query: 73  VNQKLSLLEALTGKTLNFTTLDGRNLTT-VTDIVKPGYEVVIQNEGMPISKEPNKKGNLR 132
           + QK+ L+EALTG T   +TLDGR++T  + +++ P YE V++ EGMPI K+P+KKGNLR
Sbjct: 238 MTQKIPLVEALTGYTAQVSTLDGRSVTVPINNVISPSYEEVVKGEGMPIPKDPSKKGNLR 297

Query: 133 IKFDIVFPSKLTFDQKSDLRRALGGS 158
           IKF + FPS+LT +QKS ++R    S
Sbjct: 298 IKFTVKFPSRLTTEQKSGIKRMFSSS 323

The following BLAST results are available for this feature:

BLAST of Carg17233 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013179.1	1.4e-83	100.00	DnaJ-like subfamily B member 4, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_022945456.1	9.8e-77	100.00	dnaJ homolog subfamily B member 1-like [Cucurbita moschata]	[more]
XP_022968262.1	9.8e-77	100.00	dnaJ homolog subfamily B member 1-like [Cucurbita maxima]	[more]
XP_023541690.1	3.7e-76	99.32	dnaJ homolog subfamily B member 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_008446295.1	2.7e-74	96.58	PREDICTED: dnaJ homolog subfamily B member 1 [Cucumis melo] >KAA0034375.1 dnaJ-l...	[more]

BLAST of Carg17233 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9D832	1.1e-33	47.92	DnaJ homolog subfamily B member 4 OS=Mus musculus OX=10090 GN=Dnajb4 PE=1 SV=1	[more]
Q9UDY4	6.9e-33	46.53	DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1	[more]
Q5R8J8	6.9e-33	46.53	DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1	[more]
Q2KIT4	2.0e-32	45.14	DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1	[more]
Q9QYJ3	4.5e-32	42.75	DnaJ homolog subfamily B member 1 OS=Mus musculus OX=10090 GN=Dnajb1 PE=1 SV=3	[more]

BLAST of Carg17233 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HT51	4.8e-77	100.00	dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114675...	[more]
A0A6J1G0W9	4.8e-77	100.00	dnaJ homolog subfamily B member 1-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7SYT8	1.3e-74	96.58	DnaJ-like protein subfamily B member 1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BFJ0	1.3e-74	96.58	dnaJ homolog subfamily B member 1 OS=Cucumis melo OX=3656 GN=LOC103489072 PE=4 S...	[more]
A0A0A0KSI9	2.9e-74	95.89	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G604140 PE=4 SV=1	[more]

BLAST of Carg17233 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59725.1	5.8e-59	74.66	DNAJ heat shock family protein	[more]
AT1G10350.1	4.9e-58	71.92	DNAJ heat shock family protein	[more]
AT3G47940.1	1.2e-53	64.75	DNAJ heat shock family protein	[more]
AT2G20560.1	6.2e-53	65.97	DNAJ heat shock family protein	[more]
AT3G08910.1	5.8e-51	60.96	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.60.260.20		coord: 3..59 e-value: 1.2E-17 score: 65.7
None	No IPR available	GENE3D	2.60.260.20		coord: 61..140 e-value: 4.6E-25 score: 89.8
None	No IPR available	PANTHER	PTHR24078	DNAJ HOMOLOG SUBFAMILY C MEMBER	coord: 11..156
None	No IPR available	PANTHER	PTHR24078:SF175	DNAJ HEAT SHOCK FAMILY PROTEIN	coord: 11..156
None	No IPR available	CDD	cd10747	DnaJ_C	coord: 16..142 e-value: 1.14133E-40 score: 132.164
IPR002939	Chaperone DnaJ, C-terminal	PFAM	PF01556	DnaJ_C	coord: 15..139 e-value: 6.9E-38 score: 130.2
IPR008971	HSP40/DnaJ peptide-binding	SUPERFAMILY	49493	HSP40/DnaJ peptide-binding domain	coord: 15..62
IPR008971	HSP40/DnaJ peptide-binding	SUPERFAMILY	49493	HSP40/DnaJ peptide-binding domain	coord: 63..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17233-RA	Carg17233-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051085	chaperone cofactor-dependent protein refolding
biological_process	GO:0006457	protein folding
cellular_component	GO:0005829	cytosol
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0051082	unfolded protein binding