Carg16949 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16949
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionE3 ubiquitin ligase BIG BROTHER-like
LocationCarg_Chr10: 4875482 .. 4880474 (+)
RNA-Seq ExpressionCarg16949
SyntenyCarg16949
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTTCTTTCCATTTCTTCTCCGATTTCATTTTCTTATAGTTTTCGAGCGCCCCTTTTGTTTGCTTTTGTTTGTTTCCTTCGCCCCTTTGCCTTCCTTTCTCTTCTTCTACACCTTCCCTTTACTTGTTCATCCAATTGGGGTCTTTTTCTGCTGACCCAGATGTTCTTCTGAGCTTTTGGTTCTTGGGATTTGGCGTCGGAGTTGCTTGGTGGATTCTCCGAATCGCTCCGGCGTTTTAAATGTGAGTTTGAAATAACCTACATTTTGGGTTTTCTGTTTGTGTTCAAAGAATTGCTTGCGAAATGATGAAGGAATGTGGTACTCTTTGATGGTTCTTCTTTGGTTGATTGTTGCATGATTTTGTAGAACTTGGATTCTTTCAGTTTTCTTATGGGATCTGCTAGCTTAGAGATTTGATGAGACAGGTTTTGGCTATGGCGGATAGTTTTAGGCGAAATTTCTTGACTGATCTGTGTAATTTGTGTTTGTGTTTACTGAATTACTGCTGCTTCTTCAATCTGTGATATCATAAACTCTATGTTCTTGTAATTCATGTCCTGTGTCTCACATTTAGGGGTAAAATAAAACTGGGTTTGTTGGATGAAAGTCCCACCTCGAGAATGATCCTGGGTTTATAATCAAAGAATGCTATCTCCATTGGTATGAGGTCTTTTGGGGAAGCCCATAGCAAAGCCATGAGAGTCGAGTCTCGATTAAGGGCCATGCCCTAAACTTAGCCATGCCAATAGAATCCTCAAATGTCGAAAAAAGTATTGAGTCTCAAAAAATGACTAAGACTCCAAGAAGAAAAAGAGTTAGAGCCTCGATTAAGGGGAGGCGTACTTTGTTCGAGGGGAGGTGTTGGATGAAAGTCCCACGTCGGATAATTTAGAGAATGATCATGGGTTTATAATCAAGGAATGCTATCTCTATTGGTATGAGGCCTTTTGGAGAAGCCCAAAGCAAAGCCATGAGAGCTCATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCATCTAACCGGGTTTACCGGGTTTACTCCCGTTTCTGCTTGGAACAATTCAGTTTGATGAACTCTTGTAGTGGATTTTGGCTTTGCTGAACTGTTTGGAGTGGCTTAGTATTCATTGGGTTTTGTGATGTTTCTGCATTGTACAGAATATTTGTTGGATTCATTTGTATATGAAATGAGTTGGAACAACACGCATACAGAAGAAGTTCATCATGTTAATACCAGTCATCCTTATAACAGTGCTGGAAGTTTTCTGGAATATTTTGAAGGTCTTACCTACGAACACGTAAATTTCATTTTTTCGGATGCTTCACATTCTCAGGTACTTCAGATCTGCTTGCTTCTGTGTTCTAAACTCCTGAACTTGATTTGTTCCTTGCTCTTGAACTTTTGAATTGGGAGTTTTTTTTTGTTGTTACACTGTTTCTGTCGTTCAAATTTACCGAACATCATCGTCCTCGCAGCTTATGATTATTTTGTTTTTTTGTCTTTTTTGTTTTTCCTGACCTTCCTTTTGTAACGGTCATTGTCTTCTTTGCGCTTTCCCTTTTGGGCTTCCCCTCAAGGTTTTTAAAACGTGTCTACTAGGGAGAGGCTTCCACACTCTTATAAAGGGTGTTTTGTTCTCCTCCCCAACCAATGTGGGATCTCACAATCCACCCCCTTCGGGGCCTGACACTTGTTCTCTTCTCCAATCGATGTGGGACCCCTCAATCCACCCCCATTTGGGCCTAGTGTCCTTGCTGGCACACCGCCTCGTGTCCACCCCCTTCGAGGCTCTGCCTCCTCATTGACACATCGGCCGATGTCTGGCTTTGATACCATTGGTAATAGTTCAAGCCCACCGTTAGCAAATATGTCTTATTTGGGCTTTCCCTTCGAGGTTTTAAAACACATCTGTTTGGGAGAGGTTTCCACACCCTTATAAATGATGTTTCGTTCTCCTCCCCAACCGACGTGGGATTTCACACCTTTTGAGTCGAATCTTCTTTCAAGTTGAAAGTTGTCTTCCTCTCTTTATAGCTTATTAGCTACCAAAACAATTTCAAATTCAACCATAGTCTGCTCTCTATAGTTGCTGTAATTAATTAGTTTTGATTGTAAATGGGAAATGACAATGTAACTTATTAAGTTTCACACTTGCAGGAGAATGTTTACCCTCCGCTGAACTCGAACTTCTATAAGTTCGGGTTTTCAGATTTTGGTAGTCCAACCTATTACAACCAACCTCAAGCTTATAGAATGCATGATCACGAACCCGTAATTTATGAACATAGACGGCTAGAAAACTTTTCTACTCAGCCTAATGTACAGAGTACAGTAAATCCTGAAATTGAAGAAATTGCAAATACCACAGCATTTGACAATCATGTAGAATGTAAGTGTTACTTCTCAGCATCATCCAAAAGGGTTTTCTAATTGTTTAATATCTGTTGGGTTCACCATTGAGATTTCCTACAAGTATGCAAAATATTTACTTCTGTAACGACGTTCTAGTAGAAGTGACACGTACTACTTCATTGAACATAACTGAAATGAATACATAAATCGGATGAACGTTACGTCTGGAAAAAAACACCGCTCGTTGAAGATTTCTATAAGTTTTTGTTTAGTCGAACACCAAAAAGGAATATGGGTCTTGAAACACGATCTCCCTTGAATTTCTAGTCATCGCGTTACCTCTAAATGATTCGTAGTTTTCCTCTCTAGCACTAATGACAACTTTTCTCTCAGGTCCAAGGAGACATCGTAATTCACATGATTTTCAGGTTTGTGGGTTTTATATGGTTTGACAAATCAACTTTCTACTTGAAACATGTTTGGCAGGGTGTTTTTTTGTTAATCTCAAGATCATTGATGAATGGGTCTTTACCTTTAAGTGTAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTACGAGGTTAGTGCATTTGACAAAGTTTTTGTTAACTTGTCTTCTTCTTTTTATTTGTAAGTGAAAGTATCAATTTCCATCAATATATGGAACCTCATAGTCAGAAGAACCAAGTAATGGGGACAGATAAACCATGGGAATTCTAATATCAATTTTAAGATTTACCAAATTCTTGACGCTACCTTTGATATTAAACAAGGATTTGAACTAGACGAGATAGAATAACTGCAGGCACTTGGAAAAATTCTTGGTTGAGGACATGCCAAAACGTGATGAGACATTAAAATATCTATGATTATTGGGTAATTAGTTTGATAAGGATACGCCAACCTAAGCGTAGGCCAATGGGCATCCTCGACTAGGAGGAGATCCCACATCGGATGGGGAGGAGAACGAAATGTTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAAACGCGTTTTAAAAACCTTGAGGAAAAGCCCAAGAGAGGACAATATCTATTAGAAGTGGGTTTGGGCCATTACAAATGGTATCAGAGTCAGACACCAGACGATGTGCCAACGAGGAGACTGAGTCCTGAAGGGGGTGGATTGAGGGTCCTACATTGATTGGAGAAGGGAATGAGTGGCAACAAGAACTCTGGGTCCTAAAGGAGGGTGGATTGTGAGATCTCACATAGGTTGGGGAGGAGAACGAAACATTCTTTAAGGGCGTGGAAACTTCTCCCTAACAGATGCGTTTTCAAAACCTTGAGGAGAAGCCCAAAAGGGAAAGCCCAAAGAGAACAATATCTGCTAGTAGTGGGCTTGGGCCGTTACCTTTCGAATCCCCCACCCAATGTTATTGAATTCAGAAAATTTGTTTGATAAGATAATGGGCTCTGTGATAGGCCTGCCTGCTGAACTATTATCCTGAGGTCATGATGTTATGTCTGTTTTTCGAACTAATGCTAAACAAACTTTATACTATGTGTCATCCGATCTAATCGTCTTCGAATGTATGCATTTTTTTCTTCAATCTTTGTATAAAAATCTACAATATTTCAAGTTCAGGAATTACTAGAGTTGGGCGAGACAGTAGGAACTCAAAGTCGAGGTCTTTCGCAAGAGTTGATCGCTTTGATTCCAATATCAAAATACAAATATAGTTTCTTCTCGAGGAAAAAATTTCGTGGAGAGAGGTATATCTTAGTTCATGATGTGTGCATTTATTGGGATATAAGTTCCAGTTTGTTTCTTTTTCCTGCTGAGTGAGTCTAAGTTTGTTCCTCTTACTTGGATGTAGATGTGTGATATGCCAGATGGATTATAAACGAGGCGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCCGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGTATGAAACTTAAGAGTATACCGCTTCTACGAATAACTCTAAACCGAGACCAAAGCCCTTTAGGAGTGTTTTGAGTGAGTAATTTTATGGTTTGTTGTGCAGGCTTGCCCCATATGCTACAAAGAGGTGTTTGGCGATACATCAAAACGCTAAGCCTTTCCCTTCATTGTTTAGATGACAGAAGAACAGAACCTTGTTATTACAATTTTTGTGAAAATATGGCGTGCCAAAGTGAATAAAGATTAGTTCCTTGTGTTAGCTTTATCAGTGTTTGGATTGGGTTTGATATCTCTTGAGTCTTGGTCTCGGGGCGAGCGCCCGAGCGCTCTCGAGTATAGGGAGCAAAGCTTCGAATCAATGAGATATTTTCTATCGTTTTTGTTACTTTGTAGTTACGATTGATGTTTCTTTTATTGTTATATTTTTAAATTTAAATATATTCCTTATTAATTAGTTTTATATCCTCGATCAAAAGGTTCGAGCTGCTACCTTGCTTGTTAAACTCAAGAGGTATATATAATAAGTCATCACCGTCCCCTATTTCGTACTCGTTCATATTTTCCTATCTGATACTCTTTTCCAAGTTGTCTACTTTTCCTTTCCCGGAGTCTTATTTATTTACTTATTCTCTTACGTAATTATTTTTTTTTGAGTTCAAACGAGACGT

mRNA sequence

CCTTTTCTTTCCATTTCTTCTCCGATTTCATTTTCTTATAGTTTTCGAGCGCCCCTTTTGTTTGCTTTTGTTTGTTTCCTTCGCCCCTTTGCCTTCCTTTCTCTTCTTCTACACCTTCCCTTTACTTGTTCATCCAATTGGGGTCTTTTTCTGCTGACCCAGATGTTCTTCTGAGCTTTTGGTTCTTGGGATTTGGCGTCGGAGTTGCTTGGTGGATTCTCCGAATCGCTCCGGCGTTTTAAATCTTAGAGATTTGATGAGACAGGTTTTGGCTATGGCGGATAGTTTTAGGCGAAATTTCTTGACTGATCTGTAATATTTGTTGGATTCATTTGTATATGAAATGAGTTGGAACAACACGCATACAGAAGAAGTTCATCATGTTAATACCAGTCATCCTTATAACAGTGCTGGAAGTTTTCTGGAATATTTTGAAGGTCTTACCTACGAACACGTAAATTTCATTTTTTCGGATGCTTCACATTCTCAGGAGAATGTTTACCCTCCGCTGAACTCGAACTTCTATAAGTTCGGGTTTTCAGATTTTGGTAGTCCAACCTATTACAACCAACCTCAAGCTTATAGAATGCATGATCACGAACCCGTAATTTATGAACATAGACGGCTAGAAAACTTTTCTACTCAGCCTAATGTACAGAGTACAGTAAATCCTGAAATTGAAGAAATTGCAAATACCACAGCATTTGACAATCATGTAGAATGTCCAAGGAGACATCGTAATTCACATGATTTTCAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTACGAGGAATTACTAGAGTTGGGCGAGACAGTAGGAACTCAAAGTCGAGGTCTTTCGCAAGAGTTGATCGCTTTGATTCCAATATCAAAATACAAATATAGTTTCTTCTCGAGGAAAAAATTTCGTGGAGAGAGATGTGTGATATGCCAGATGGATTATAAACGAGGCGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCCGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGCTTGCCCCATATGCTACAAAGAGGTGTTTGGCGATACATCAAAACGCTAAGCCTTTCCCTTCATTGTTTAGATGACAGAAGAACAGAACCTTGTTATTACAATTTTTGTGAAAATATGGCGTGCCAAAGTGAATAAAGATTAGTTCCTTGTGTTAGCTTTATCAGTGTTTGGATTGGGTTTGATATCTCTTGAGTCTTGGTCTCGGGGCGAGCGCCCGAGCGCTCTCGAGTATAGGGAGCAAAGCTTCGAATCAATGAGATATTTTCTATCGTTTTTGTTACTTTGTAGTTACGATTGATGTTTCTTTTATTGTTATATTTTTAAATTTAAATATATTCCTTATTAATTAGTTTTATATCCTCGATCAAAAGGTTCGAGCTGCTACCTTGCTTGTTAAACTCAAGAGGTATATATAATAAGTCATCACCGTCCCCTATTTCGTACTCGTTCATATTTTCCTATCTGATACTCTTTTCCAAGTTGTCTACTTTTCCTTTCCCGGAGTCTTATTTATTTACTTATTCTCTTACGTAATTATTTTTTTTTGAGTTCAAACGAGACGT

Coding sequence (CDS)

ATGAGTTGGAACAACACGCATACAGAAGAAGTTCATCATGTTAATACCAGTCATCCTTATAACAGTGCTGGAAGTTTTCTGGAATATTTTGAAGGTCTTACCTACGAACACGTAAATTTCATTTTTTCGGATGCTTCACATTCTCAGGAGAATGTTTACCCTCCGCTGAACTCGAACTTCTATAAGTTCGGGTTTTCAGATTTTGGTAGTCCAACCTATTACAACCAACCTCAAGCTTATAGAATGCATGATCACGAACCCGTAATTTATGAACATAGACGGCTAGAAAACTTTTCTACTCAGCCTAATGTACAGAGTACAGTAAATCCTGAAATTGAAGAAATTGCAAATACCACAGCATTTGACAATCATGTAGAATGTCCAAGGAGACATCGTAATTCACATGATTTTCAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTACGAGGAATTACTAGAGTTGGGCGAGACAGTAGGAACTCAAAGTCGAGGTCTTTCGCAAGAGTTGATCGCTTTGATTCCAATATCAAAATACAAATATAGTTTCTTCTCGAGGAAAAAATTTCGTGGAGAGAGATGTGTGATATGCCAGATGGATTATAAACGAGGCGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCCGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGCTTGCCCCATATGCTACAAAGAGGTGTTTGGCGATACATCAAAACGCTAA

Protein sequence

MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNFYKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTAFDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPISKYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKEVFGDTSKR
Homology
BLAST of Carg16949 vs. NCBI nr
Match: KAG7023862.1 (E3 ubiquitin ligase BIG BROTHER [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 528.9 bits (1361), Expect = 2.4e-146
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of Carg16949 vs. NCBI nr
Match: XP_022960774.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata])

HSP 1 Score: 526.2 bits (1354), Expect = 1.6e-145
Identity = 246/248 (99.19%), Postives = 247/248 (99.60%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPE EEI+NTTA
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEFEEISNTTA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of Carg16949 vs. NCBI nr
Match: XP_023515391.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.5 bits (1342), Expect = 3.8e-144
Identity = 245/248 (98.79%), Postives = 245/248 (98.79%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPP NSNF
Sbjct: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPPNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEH RLENFSTQPNVQSTVNPE EEIANTTA
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHIRLENFSTQPNVQSTVNPEFEEIANTTA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of Carg16949 vs. NCBI nr
Match: XP_022988200.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita maxima])

HSP 1 Score: 516.9 bits (1330), Expect = 9.5e-143
Identity = 243/248 (97.98%), Postives = 246/248 (99.19%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNTHT EVHHVNTSHPYN+AGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTHT-EVHHVNTSHPYNTAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPE EEIANTTA
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEFEEIANTTA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKKFRGERCVICQM+YKRGDRR+TLPCKHIYHAGCGTKWLSINKACPICYKE
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMEYKRGDRRMTLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 247

BLAST of Carg16949 vs. NCBI nr
Match: XP_038880080.1 (E3 ubiquitin-protein ligase BIG BROTHER-like isoform X2 [Benincasa hispida])

HSP 1 Score: 486.9 bits (1252), Expect = 1.0e-133
Identity = 227/248 (91.53%), Postives = 237/248 (95.56%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNT T EVHHVNT++PYN+AGSFLEYFEGLTYEHVNFIFS ASHSQENVYPPLNSNF
Sbjct: 31  MSWNNTQT-EVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 90

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSP YYNQPQAYRMHDHEP+IYEHRRLE+ +TQPN+QST+NPE EEI NT A
Sbjct: 91  YKFGFSDFGSPAYYNQPQAYRMHDHEPIIYEHRRLESSATQPNLQSTINPEFEEITNTIA 150

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRH NSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIAL+PIS
Sbjct: 151 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 210

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKK RGERCVICQM+YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY E
Sbjct: 211 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 270

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 271 VFGDTSKR 277

BLAST of Carg16949 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.9e-54
Identity = 111/250 (44.40%), Postives = 153/250 (61.20%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           M+ +N   E+ H+  T  PY + GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFS   + ++Y    +Y M+DH   +   R   ++    NV       +        
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGD 120

Query: 121 FDNHVECPRRHRNSHD---FQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALI 180
            D H E      N HD    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI  +
Sbjct: 121 TDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PISKYKY-SFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPI 240
           P  KYK+ S FSRK+  GERCVICQ+ YK G+R++ LPCKH+YH+ C +KWLSINK CP+
Sbjct: 181 PTKKYKFGSIFSRKR-AGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPV 240

Query: 241 CYKEVFGDTS 247
           C  EVFG+ S
Sbjct: 241 CNSEVFGEPS 246

BLAST of Carg16949 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.3e-25
Identity = 56/113 (49.56%), Postives = 71/113 (62.83%), Query Frame = 0

Query: 134 SHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPISKYKYSFFSRKKFR 193
           SH  Q  W D +DPD ++YEELL LG+ VGT+SRGLS + IA +P  +YK      +   
Sbjct: 228 SHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGT 287

Query: 194 GERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKEVFGDTS 247
            E CVIC++DY+  +  I LPCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 288 NESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

BLAST of Carg16949 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.7e-14
Identity = 39/94 (41.49%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALIPISKYKYSFFSRKKFRG-ERCVICQMD 204
           +D DNM+YEELL LGE +G  S GLS+E+I  + + ++K++  +    +  E C +CQ +
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKV-MKQHKHTSSAAGSHQDMEPCCVCQEE 626

Query: 205 YKRGDRRITLPCKHIYHAGCGTKWLSINKACPIC 238
           Y  GD   TL C H +H  C  +WL +   CPIC
Sbjct: 627 YAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 659

BLAST of Carg16949 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.3e-14
Identity = 43/100 (43.00%), Postives = 57/100 (57.00%), Query Frame = 0

Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALIPISKYKYSFFSRKKF----RGERCVIC 204
           +D DNM+YEELL LGE +G  S GLS+E+I L  + ++K++  S          E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 205 QMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 241
           Q +Y  GD   TL C H +H  C  +W+ I   CPIC  E
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

BLAST of Carg16949 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 3.0e-14
Identity = 45/125 (36.00%), Postives = 61/125 (48.80%), Query Frame = 0

Query: 113 EEIANTTAFDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQE 172
           E +   + F   V+   RHR+           +D DNM+YEELL L E +G  S GLS+E
Sbjct: 545 ENVRFESIFYGGVDIHDRHRDM---------RLDIDNMSYEELLALEERIGNVSTGLSEE 604

Query: 173 LIALIPISKYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINK 232
            +  +   +   S+        E C ICQ +Y  GD   TL C H +H GC  +WL +  
Sbjct: 605 EVTKLLKQRKFSSWRLEASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKN 660

Query: 233 ACPIC 238
            CPIC
Sbjct: 665 TCPIC 660

BLAST of Carg16949 vs. ExPASy TrEMBL
Match: A0A6J1HC34 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC111461475 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 7.6e-146
Identity = 246/248 (99.19%), Postives = 247/248 (99.60%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPE EEI+NTTA
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEFEEISNTTA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of Carg16949 vs. ExPASy TrEMBL
Match: A0A6J1JIX4 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111485509 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 4.6e-143
Identity = 243/248 (97.98%), Postives = 246/248 (99.19%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNTHT EVHHVNTSHPYN+AGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTHT-EVHHVNTSHPYNTAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPE EEIANTTA
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEFEEIANTTA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKKFRGERCVICQM+YKRGDRR+TLPCKHIYHAGCGTKWLSINKACPICYKE
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMEYKRGDRRMTLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 247

BLAST of Carg16949 vs. ExPASy TrEMBL
Match: A0A0A0LX98 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G613470 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 6.6e-134
Identity = 226/248 (91.13%), Postives = 236/248 (95.16%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWN+T   EVHHVNT++PYN+AGSFLEYFEGLTYEHVNFIFS ASHSQENVYPPLNSNF
Sbjct: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSP+YYN PQAYRMHDHEP+IYEHRRLEN +TQPN+QSTVNPE EEI NT A
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRH NSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIAL+PIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKK RGERCVICQM+YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY E
Sbjct: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of Carg16949 vs. ExPASy TrEMBL
Match: A0A1S3BQ88 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucumis melo OX=3656 GN=LOC103492041 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.1e-133
Identity = 225/248 (90.73%), Postives = 235/248 (94.76%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNT   EVHHVNT++PYN+AGSFLEYFEGLTYEHVNFIFS ASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSP+YYN PQAYRMHDHEP+IYEHRRLEN +TQPN+QSTVNPE EEI NT A
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPRRH NSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIAL+PIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKY FFSRKK RG+RCVICQM+YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY E
Sbjct: 181 KYKYGFFSRKKSRGDRCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of Carg16949 vs. ExPASy TrEMBL
Match: A0A6J1EIF5 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC111434637 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.6e-130
Identity = 224/247 (90.69%), Postives = 233/247 (94.33%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           MSWNNTHT EVHHVNT +PYN+AGSFLEYFEGLTYEHVNFIFS ASHSQE VYPPLNSNF
Sbjct: 1   MSWNNTHT-EVHHVNTPYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQETVYPPLNSNF 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFSDFGSPTYYN PQAYR+HD EP+IYEHRRLEN +TQP+VQSTVN E+EE ANTTA
Sbjct: 61  YKFGFSDFGSPTYYNPPQAYRVHDQEPIIYEHRRLENSATQPDVQSTVNLELEEFANTTA 120

Query: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180
           FDNHVECPR   NSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIAL+PIS
Sbjct: 121 FDNHVECPRGRHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240
           KYKYSFFSRKK RGERCVICQM+YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY E
Sbjct: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSK 248
           VFGDTSK
Sbjct: 241 VFGDTSK 246

BLAST of Carg16949 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 213.4 bits (542), Expect = 2.1e-55
Identity = 111/250 (44.40%), Postives = 153/250 (61.20%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           M+ +N   E+ H+  T  PY + GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFS   + ++Y    +Y M+DH   +   R   ++    NV       +        
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGD 120

Query: 121 FDNHVECPRRHRNSHD---FQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALI 180
            D H E      N HD    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI  +
Sbjct: 121 TDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PISKYKY-SFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPI 240
           P  KYK+ S FSRK+  GERCVICQ+ YK G+R++ LPCKH+YH+ C +KWLSINK CP+
Sbjct: 181 PTKKYKFGSIFSRKR-AGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPV 240

Query: 241 CYKEVFGDTS 247
           C  EVFG+ S
Sbjct: 241 CNSEVFGEPS 246

BLAST of Carg16949 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 213.4 bits (542), Expect = 2.1e-55
Identity = 111/250 (44.40%), Postives = 153/250 (61.20%), Query Frame = 0

Query: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60
           M+ +N   E+ H+  T  PY + GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEIEEIANTTA 120
           YKFGFS   + ++Y    +Y M+DH   +   R   ++    NV       +        
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGRTNWDYHPMVNVADDPENTVARSVQIGD 120

Query: 121 FDNHVECPRRHRNSHD---FQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALI 180
            D H E      N HD    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI  +
Sbjct: 121 TDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PISKYKY-SFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPI 240
           P  KYK+ S FSRK+  GERCVICQ+ YK G+R++ LPCKH+YH+ C +KWLSINK CP+
Sbjct: 181 PTKKYKFGSIFSRKR-AGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPV 240

Query: 241 CYKEVFGDTS 247
           C  EVFG+ S
Sbjct: 241 CNSEVFGEPS 246

BLAST of Carg16949 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 9.1e-27
Identity = 56/113 (49.56%), Postives = 71/113 (62.83%), Query Frame = 0

Query: 134 SHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPISKYKYSFFSRKKFR 193
           SH  Q  W D +DPD ++YEELL LG+ VGT+SRGLS + IA +P  +YK      +   
Sbjct: 228 SHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGT 287

Query: 194 GERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKEVFGDTS 247
            E CVIC++DY+  +  I LPCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 288 NESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

BLAST of Carg16949 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 9.5e-24
Identity = 53/108 (49.07%), Postives = 69/108 (63.89%), Query Frame = 0

Query: 143 DNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPISKYK------YSFFSRKKFRGE- 202
           +N DP+NMTYEEL ELG++VG   +GLSQE I+ +   KY       Y    +KKF  + 
Sbjct: 172 ENNDPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYCLHMKKKFVADD 231

Query: 203 -RCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKEVF 243
            +C IC M+Y +GD+  TLPCKHIYH  C ++WL  NK C IC  EV+
Sbjct: 232 SQCSICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

BLAST of Carg16949 vs. TAIR 10
Match: AT2G37150.1 (RING/U-box superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 4.7e-15
Identity = 40/104 (38.46%), Postives = 58/104 (55.77%), Query Frame = 0

Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALIPISKYKYSFFSRKKFRGERCVICQMDY 204
           +D DNM+YEELL+LGE +G+ + GLS   I+   ++   Y  +  ++ R  +C IC  +Y
Sbjct: 442 LDIDNMSYEELLDLGERIGSVNTGLSDSAISSCLLATMYYPSYQTEEQR--KCAICLEEY 501

Query: 205 KRGDRRITLP-CKHIYHAGCGTKWLSINKACPICYKEVFGDTSK 248
           K  +    +  C H YH  C  KWLS+  +CPIC      D SK
Sbjct: 502 KEKEELGEVKGCGHDYHGRCIKKWLSMKNSCPICKSPALPDASK 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023862.12.4e-146100.00E3 ubiquitin ligase BIG BROTHER [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022960774.11.6e-14599.19E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata][more]
XP_023515391.13.8e-14498.79E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo][more]
XP_022988200.19.5e-14397.98E3 ubiquitin ligase BIG BROTHER-like [Cucurbita maxima][more]
XP_038880080.11.0e-13391.53E3 ubiquitin-protein ligase BIG BROTHER-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8L6492.9e-5444.40E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE... [more]
Q9LT171.3e-2549.56E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P... [more]
O495001.7e-1441.49E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q9ZQF92.3e-1443.00E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q7XTV73.0e-1436.00Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1HC347.6e-14699.19E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC1114614... [more]
A0A6J1JIX44.6e-14397.98E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111485509... [more]
A0A0A0LX986.6e-13491.13RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G613470 P... [more]
A0A1S3BQ881.1e-13390.73E3 ubiquitin ligase BIG BROTHER-like OS=Cucumis melo OX=3656 GN=LOC103492041 PE=... [more]
A0A6J1EIF52.6e-13090.69E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC1114346... [more]
Match NameE-valueIdentityDescription
AT3G63530.12.1e-5544.40RING/U-box superfamily protein [more]
AT3G63530.22.1e-5544.40RING/U-box superfamily protein [more]
AT3G19910.19.1e-2749.56RING/U-box superfamily protein [more]
AT5G52140.19.5e-2449.07RING/U-box superfamily protein [more]
AT2G37150.14.7e-1538.46RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 197..237
e-value: 2.4E-5
score: 33.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 196..237
e-value: 4.5E-11
score: 42.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 197..237
score: 10.77793
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 159..242
e-value: 1.2E-16
score: 62.2
NoneNo IPR availablePANTHERPTHR46400:SF5RING/U-BOX SUPERFAMILY PROTEINcoord: 1..247
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 196..242
IPR033276E3 ubiquitin-protein ligase BIG BROTHERPANTHERPTHR46400RING/U-BOX SUPERFAMILY PROTEINcoord: 1..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16949-RACarg16949-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046621 negative regulation of organ growth
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0004842 ubiquitin-protein transferase activity