Carg16860 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16860
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWRKY protein
LocationCarg_Chr19: 341369 .. 343394 (+)
RNA-Seq ExpressionCarg16860
SyntenyCarg16860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAGCTTGAAGAAGCTAACAGAGCCGCCATAGATAGCTGCCATGGAGTTTTGAATCTTTTAGCTCAGCCTTCTCCTCAAGAACAAGGTGAGCTGAGGAAGAATTTAATGGTGGAAACTGGAGAGGCTGTTTTTAAGCTCAAGAAAGTGGTATCTCTTTTGAATTCTGGGTTTGGTTATGCAAAAGTTAGAAGATTCAAGAACATTCCTCTGCCTTTACCCCAAAAACTTCTCTTAGATCCTCCAAACAATGGTTTGAATGGTAAAGTTTCAGTTTTTTTGGGAAACCCAGATTTGGAAATGGATCAAAATCATAAGAATTCCCTCAAAATTCATAAACAAGCTGCTCCATCTTTGAACTTTAGCTTCCCCCAGCAACAGCAGCAGCAGAGGAAGCAAGCAGAGATGATGTTTCTTAGGAGTAACAGTGGGATGAATATGAATTTTGATGCTTCTAAGTGCACATTGACAATGTCATCTGCTAGATCTTTCATTTCTTCGTTGAGTATGGACGGGAGTGTTGCGGACGGGAGCTCGTTCCATTTGATCGGGCCGTCGTCGACGATGTCGGGCGATAACAAGAGGAGGTTTTCTGGAAGGGGAGATGAAGGGAGCTTGAAATGTGGTAGCACTGGCAAGTGCCATTGCTCAAAGAAGAGGTTGGATTGATATCCATCTTGTTGGGTGTTGATATCCATCTTGTTGGGTGTTGATATCCCTTTGGTTTACTGATAATTTGGGTTGTTTTTGTAGGAAACATCGAGTGAAGAGATCAATTAAGGTTCCTGCTATAAGTAACAAACTTGCTGATATCCCTTCTGATGATTATTCATGGAGGAAATATGGGCAGAAGCCAATTAAGGGTTCTCCTCATCCCAGGTATGATTAAATCTCGGGTCGGTCCTAACTGATATTGTCCACTTCGTGCATAATTCTAGCCTTTTGACTCTATCTCAATGGTGTGTTTACTTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAGGATTCCTTACAAGGGTGTGAAAACCTCTCCCTAACAGACGCAATGGTAATATGTAATGGGTCAAAACGGACAATATCTACTAGCAGTGGCCTTGAACTATTACAAATGGTATTAAAACCAGACACCGGATGGTGTGCCAGCGAGAACGCTGGGCTCCAAGGGGGTGGATTATGAGATCCCACATCAGTTAGAGAGGGAAACAAAATATTCCTTATGAAGATGTGGAAACCTCTCACCAACAGACCGTTTTAAAACTATGAGGATGATGGTGATACGTTACCGGCGACAGCGGACAATATTTGTTAGCGGTGGGCTTGGGCTGTTACAAATGGTATTAGAGTTAGATGGTGTGCCAGCGAAGACGCTGGGCACCAAAGGGTGGATTGTGAGATCTCATATAGGTGGAAAAGGGGAACAACGCATTCCTTATAAGGGTGTGAAAACCTCTCCCTAGCATACATGTTTTAAAACTATGAGGCTGACGACGATACGTAACGAGCCAAAGCAGACAATATCTGCTAGCGGTGGGTTATAATAAGCTTACAATGACTTCTACTTCCGATAATCATGGTTAAAGAGGTGTCTCTTTGCTAAATCATTAGAACAAGATGTTCATATAACAGGATCATTACTAAGAAAATGGTACAAGTTTTTGTAATTTTTCGACAACTCGAAACACACGAAAACTTCGGGCGATTGTCAAACCGAATGGATGCCTGTTTTATGAAGATTGCTGAAGAATACAAATCTTTTCCATTTTCGTGTCGTTGGATCATTTCGAACTCGAAAAATTGAGGATTTGAGAGAGGGAAAGTGTTGATGATTGAGGAAGTTTGTAAAATGAGTTTGTTTTTGTTGTAAAATGTGTGCAGGGGTTACTACAAATGCAGCAGCATGAGAGGTTGTCCAGCGAGAAAGCACGTCGAGCGATGCTTAGAAGACCCGTCGATGCTTATCGTAACGTACGAAGGAGAGCATAATCACCCGAAAATGTCGACGCAATCTGCACACACTTAG

mRNA sequence

ATGGAAGAGCTTGAAGAAGCTAACAGAGCCGCCATAGATAGCTGCCATGGAGTTTTGAATCTTTTAGCTCAGCCTTCTCCTCAAGAACAAGGTGAGCTGAGGAAGAATTTAATGGTGGAAACTGGAGAGGCTGTTTTTAAGCTCAAGAAAGTGGTATCTCTTTTGAATTCTGGGTTTGGTTATGCAAAAGTTAGAAGATTCAAGAACATTCCTCTGCCTTTACCCCAAAAACTTCTCTTAGATCCTCCAAACAATGGTTTGAATGGTAAAGTTTCAGTTTTTTTGGGAAACCCAGATTTGGAAATGGATCAAAATCATAAGAATTCCCTCAAAATTCATAAACAAGCTGCTCCATCTTTGAACTTTAGCTTCCCCCAGCAACAGCAGCAGCAGAGGAAGCAAGCAGAGATGATGTTTCTTAGGAGTAACAGTGGGATGAATATGAATTTTGATGCTTCTAAGTGCACATTGACAATGTCATCTGCTAGATCTTTCATTTCTTCGTTGAGTATGGACGGGAGTGTTGCGGACGGGAGCTCGTTCCATTTGATCGGGCCGTCGTCGACGATGTCGGGCGATAACAAGAGGAGGTTTTCTGGAAGGGGAGATGAAGGGAGCTTGAAATGTGGTAGCACTGGCAAGTGCCATTGCTCAAAGAAGAGGAAACATCGAGTGAAGAGATCAATTAAGGTTCCTGCTATAAGTAACAAACTTGCTGATATCCCTTCTGATGATTATTCATGGAGGAAATATGGGCAGAAGCCAATTAAGGGTTCTCCTCATCCCAGGGGTTACTACAAATGCAGCAGCATGAGAGGTTGTCCAGCGAGAAAGCACGTCGAGCGATGCTTAGAAGACCCGTCGATGCTTATCGTAACGTACGAAGGAGAGCATAATCACCCGAAAATGTCGACGCAATCTGCACACACTTAG

Coding sequence (CDS)

ATGGAAGAGCTTGAAGAAGCTAACAGAGCCGCCATAGATAGCTGCCATGGAGTTTTGAATCTTTTAGCTCAGCCTTCTCCTCAAGAACAAGGTGAGCTGAGGAAGAATTTAATGGTGGAAACTGGAGAGGCTGTTTTTAAGCTCAAGAAAGTGGTATCTCTTTTGAATTCTGGGTTTGGTTATGCAAAAGTTAGAAGATTCAAGAACATTCCTCTGCCTTTACCCCAAAAACTTCTCTTAGATCCTCCAAACAATGGTTTGAATGGTAAAGTTTCAGTTTTTTTGGGAAACCCAGATTTGGAAATGGATCAAAATCATAAGAATTCCCTCAAAATTCATAAACAAGCTGCTCCATCTTTGAACTTTAGCTTCCCCCAGCAACAGCAGCAGCAGAGGAAGCAAGCAGAGATGATGTTTCTTAGGAGTAACAGTGGGATGAATATGAATTTTGATGCTTCTAAGTGCACATTGACAATGTCATCTGCTAGATCTTTCATTTCTTCGTTGAGTATGGACGGGAGTGTTGCGGACGGGAGCTCGTTCCATTTGATCGGGCCGTCGTCGACGATGTCGGGCGATAACAAGAGGAGGTTTTCTGGAAGGGGAGATGAAGGGAGCTTGAAATGTGGTAGCACTGGCAAGTGCCATTGCTCAAAGAAGAGGAAACATCGAGTGAAGAGATCAATTAAGGTTCCTGCTATAAGTAACAAACTTGCTGATATCCCTTCTGATGATTATTCATGGAGGAAATATGGGCAGAAGCCAATTAAGGGTTCTCCTCATCCCAGGGGTTACTACAAATGCAGCAGCATGAGAGGTTGTCCAGCGAGAAAGCACGTCGAGCGATGCTTAGAAGACCCGTCGATGCTTATCGTAACGTACGAAGGAGAGCATAATCACCCGAAAATGTCGACGCAATCTGCACACACTTAG

Protein sequence

MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFGYAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSLNFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSSFHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT
Homology
BLAST of Carg16860 vs. NCBI nr
Match: XP_022963451.1 (probable WRKY transcription factor 21 [Cucurbita moschata] >AZZ86683.1 WRKY21 [Cucurbita moschata] >KAG6571488.1 putative WRKY transcription factor 21, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011250.1 putative WRKY transcription factor 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620.2 bits (1598), Expect = 9.9e-174
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG
Sbjct: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120
           YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL
Sbjct: 61  YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120

Query: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180
           NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS
Sbjct: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180

Query: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240
           FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD
Sbjct: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240

Query: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300
           IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH
Sbjct: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300

Query: 301 PKMSTQSAHT 311
           PKMSTQSAHT
Sbjct: 301 PKMSTQSAHT 310

BLAST of Carg16860 vs. NCBI nr
Match: XP_022967410.1 (probable WRKY transcription factor 21 [Cucurbita maxima])

HSP 1 Score: 617.1 bits (1590), Expect = 8.4e-173
Identity = 307/310 (99.03%), Postives = 309/310 (99.68%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG
Sbjct: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120
           YAKVRRFKNIPLPLPQK LLDPPNNGLNGK+SVFLGNPDLEMDQNHKNSLKIHKQAAPSL
Sbjct: 61  YAKVRRFKNIPLPLPQKFLLDPPNNGLNGKISVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120

Query: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180
           NFSFPQQQQQQRKQAEMMF+RSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS
Sbjct: 121 NFSFPQQQQQQRKQAEMMFVRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180

Query: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240
           FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD
Sbjct: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240

Query: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300
           IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH
Sbjct: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300

Query: 301 PKMSTQSAHT 311
           PKMSTQSAHT
Sbjct: 301 PKMSTQSAHT 310

BLAST of Carg16860 vs. NCBI nr
Match: XP_023554095.1 (probable WRKY transcription factor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.3 bits (1588), Expect = 1.4e-172
Identity = 307/310 (99.03%), Postives = 308/310 (99.35%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEELEEANRAAIDSCHGVLNLLA PSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG
Sbjct: 1   MEELEEANRAAIDSCHGVLNLLAHPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120
           YAKVRRFKNIPLPLPQK LLDPPNNGLNGK+SVFLGNPDLEMDQNHKNSLKIHKQAAPSL
Sbjct: 61  YAKVRRFKNIPLPLPQKFLLDPPNNGLNGKISVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120

Query: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180
           NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS
Sbjct: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180

Query: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240
           FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD
Sbjct: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240

Query: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300
           IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH
Sbjct: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300

Query: 301 PKMSTQSAHT 311
           PKMSTQSAHT
Sbjct: 301 PKMSTQSAHT 310

BLAST of Carg16860 vs. NCBI nr
Match: XP_038886893.1 (probable WRKY transcription factor 21 isoform X1 [Benincasa hispida])

HSP 1 Score: 489.6 bits (1259), Expect = 2.0e-134
Identity = 266/339 (78.47%), Postives = 284/339 (83.78%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+EEAN+AAI+SCHGVLNLL QPS Q+Q   R NLMVET EAVFK KKV+SLLNSGFG
Sbjct: 1   MEEVEEANKAAIESCHGVLNLLIQPS-QDQLHFR-NLMVETKEAVFKFKKVISLLNSGFG 60

Query: 61  YAKVRRFKNIPLPLPQKLLL-------DPPNN-------GLNGKVSVFLGNPDLEMDQNH 120
           +A+VR F  IPLPLP+K LL       DPPN        GLNGKVS+FLGNPDLE+  N 
Sbjct: 61  HARVRNFNKIPLPLPKKSLLDSLNYTSDPPNKNLYPFPPGLNGKVSIFLGNPDLELSPND 120

Query: 121 KNSLKIHKQAAPSLNFSFPQQQQQQ-----------RKQAEMMFLRSNSGMNMNFDASKC 180
           KNSL+I KQ  PSLNFSFPQQQQQQ           ++QAEMMFLR+N+GMN+NFD S  
Sbjct: 121 KNSLQIPKQ-TPSLNFSFPQQQQQQQQRILAHQKQMKQQAEMMFLRNNNGMNLNFDTSNG 180

Query: 181 TLTMSSARSFISSLSMDGSV--ADGSSFHLIGPS--STMSGDNKRRFSGRGDEGSLKCGS 240
           TLTMSSARSFISSLSMDGSV  AD SSFHLIGPS  +T S DNKR+FS RGDEGSLKCGS
Sbjct: 181 TLTMSSARSFISSLSMDGSVVGADRSSFHLIGPSMTTTTSADNKRKFSARGDEGSLKCGS 240

Query: 241 TGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM 300
           TGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM
Sbjct: 241 TGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM 300

Query: 301 RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 311
           RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT
Sbjct: 301 RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 336

BLAST of Carg16860 vs. NCBI nr
Match: QIS68546.1 (WRKY20 protein [Cucumis metulifer])

HSP 1 Score: 474.2 bits (1219), Expect = 8.8e-130
Identity = 251/339 (74.04%), Postives = 271/339 (79.94%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+EEAN++AI+SCHGVLNLL QP P       KNLM+ET EAVFK KKV+SLLNS F 
Sbjct: 1   MEEVEEANKSAIESCHGVLNLLLQPPPHHHQHHFKNLMLETKEAVFKFKKVISLLNSDFN 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPPN--------------NGLNGKVSVFLGNPDLEMDQNH 120
           + + R F  IPLPLPQ  LLD PN               G N KVS+FLGNPDLE+ QN 
Sbjct: 61  HPRFRNFNKIPLPLPQNSLLDSPNYTLHPPNKNLFTFPPGFNSKVSIFLGNPDLELSQND 120

Query: 121 KNSLKIHKQAAPSLNFSFPQQQQQQ-----------RKQAEMMFLRSNSGMNMNFDASKC 180
           KNSL I KQ +PSLNFSFP   QQQ           ++QAEMMFLR+N+GMN+NFD S C
Sbjct: 121 KNSLHIPKQ-SPSLNFSFPHHHQQQQQSVLAHQKQMKQQAEMMFLRNNNGMNLNFDTSNC 180

Query: 181 TLTMSSARSFISSLSMDGSV-ADGSSFHLIGPS---STMSGDNKRRFSGRGDEGSLKCGS 240
           TLTMSSARSFISSLSMDGSV  D SSFHLIGPS   +T SGD+KR+FS RG+EGSLKCGS
Sbjct: 181 TLTMSSARSFISSLSMDGSVIGDRSSFHLIGPSTTTTTTSGDSKRKFSARGEEGSLKCGS 240

Query: 241 TGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM 300
           T KCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM
Sbjct: 241 TSKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM 300

Query: 301 RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 311
           RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT
Sbjct: 301 RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 338

BLAST of Carg16860 vs. ExPASy Swiss-Prot
Match: O04336 (Probable WRKY transcription factor 21 OS=Arabidopsis thaliana OX=3702 GN=WRKY21 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 7.6e-84
Identity = 188/380 (49.47%), Postives = 237/380 (62.37%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+E  NRAA++SCH VLNLL +   Q+     KNL+ ET EAV + K+V SLL+S  G
Sbjct: 1   MEEIEGTNRAAVESCHRVLNLLHRSQQQDHVGFEKNLVSETREAVIRFKRVGSLLSSSVG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDP--------PNNG----------------LNGKVSVFLG 120
           +A+ RR K +   + Q LLLDP        P++                      S+ LG
Sbjct: 61  HARFRRAKKLQSHVSQSLLLDPCQQRTTEVPSSSSQKTPVLRSGFQELSLRQPSDSLTLG 120

Query: 121 NPDLEMDQNHKNS-LKIHKQAAPSLNFS--FPQQQQQQRK-------------------- 180
                ++ N K   L++++Q  P  N+   FP QQQQQ++                    
Sbjct: 121 TRSFSLNSNAKAPLLQLNQQTMPPSNYPTLFPVQQQQQQQQQQQQQEQQQQQQQQQQQFH 180

Query: 181 -----------------QAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVA 240
                            QAE+M  + N G++++FD S CT TMSS RSF+SSLS+DGSVA
Sbjct: 181 ERLQAHHLHQQQQLQKHQAELMLRKCNGGISLSFDNSSCTPTMSSTRSFVSSLSIDGSVA 240

Query: 241 D---GSSFHLIGPSST--MSGDNKRRFSGRGDE-GSLKCGSTGKCHCSKKRKHRVKRSIK 300
           +    +SFH   PSST   S  +KR+   +GDE GSLKCGS+ +CHC+KKRKHRV+RSI+
Sbjct: 241 NIEGKNSFHFGVPSSTDQNSLHSKRKCPLKGDEHGSLKCGSSSRCHCAKKRKHRVRRSIR 300

Query: 301 VPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSML 311
           VPAISNK+ADIP DDYSWRKYGQKPIKGSP+PRGYYKCSSMRGCPARKHVERCLEDP+ML
Sbjct: 301 VPAISNKVADIPPDDYSWRKYGQKPIKGSPYPRGYYKCSSMRGCPARKHVERCLEDPAML 360

BLAST of Carg16860 vs. ExPASy Swiss-Prot
Match: Q93WU6 (Probable WRKY transcription factor 74 OS=Arabidopsis thaliana OX=3702 GN=WRKY74 PE=2 SV=2)

HSP 1 Score: 287.0 bits (733), Expect = 2.6e-76
Identity = 178/338 (52.66%), Postives = 218/338 (64.50%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+E AN+AA++SCHGVLNLL+Q     Q    K++MVET EAV K K+V SLL+ G G
Sbjct: 1   MEEVEAANKAAVESCHGVLNLLSQ-----QTNDSKSIMVETREAVCKFKRVSSLLSRGLG 60

Query: 61  YAKVRRFKNIPLP-----LPQKLLLDPP---NNGLNGKVSVF---------LGNPDLEM- 120
             K+++  N         LPQ + L+ P   NN ++G + +           G P L + 
Sbjct: 61  QRKIKKLNNNNYKFSSSLLPQHMFLESPVCSNNAISGCIPILAPKPLQIVPAGPPPLMLF 120

Query: 121 DQNHKNSLKIHKQAAPSLNFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDAS---KC--TL 180
           +QN        +   PS     P+  Q      + ++ RS SG+N+ FD S    C    
Sbjct: 121 NQNMCLDKSFLELKPPSSRAVDPKPYQFIHTHQQGVYSRSKSGLNLKFDGSIGASCYSPS 180

Query: 181 TMSSARSFISSLSMDGSVA--DGSSFHLIG--PSSTMSGDNKRRFSGRGDEGSLKCGSTG 240
             + +RSF+SSLSMDGSV   D +SFHLIG    S     + RR S     GSLKCGS  
Sbjct: 181 ISNGSRSFVSSLSMDGSVTDYDRNSFHLIGLPQGSDHISQHSRRTS---CSGSLKCGSKS 240

Query: 241 KCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG 300
           KCHCSKKRK RVKRSIKVPAISNK+ADIP D+YSWRKYGQKPIKGSPHPRGYYKCSS+RG
Sbjct: 241 KCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSSVRG 300

Query: 301 CPARKHVERCLEDPSMLIVTYEGEHNHPK-MSTQSAHT 311
           CPARKHVERC+E+ SMLIVTYEGEHNH + +S+QSAHT
Sbjct: 301 CPARKHVERCVEETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of Carg16860 vs. ExPASy Swiss-Prot
Match: Q9SR07 (Probable WRKY transcription factor 39 OS=Arabidopsis thaliana OX=3702 GN=WRKY39 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.4e-76
Identity = 173/339 (51.03%), Postives = 209/339 (61.65%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+E ANR+AI+SCHGVLNLL+Q +        K+L VETGE V K K+V SLL  G G
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLSQRTSDP-----KSLTVETGEVVSKFKRVASLLTRGLG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPP---NNGLNGKVSVFLGNPDLEM---DQNHKNSLKIHK 120
           + K R         PQ + L+ P    N L+G  +  L    L+M      +      H+
Sbjct: 61  HGKFRSTNKFRSSFPQHIFLESPICCGNDLSGDYTQVLAPEPLQMVPASAVYNEMEPKHQ 120

Query: 121 QAAPSLNFS----------------FPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTL- 180
              PSL  S                F    Q      ++ + RSNSG+N+ FD S  +  
Sbjct: 121 LGHPSLMLSHKMCVDKSFLELKPPPFRAPYQLIHNHQQIAYSRSNSGVNLKFDGSGSSCY 180

Query: 181 ---TMSSARSFISSLSMDGSVA--DGSSFHLIGPSSTMSGDNKRRFSGRGDEGSLKCGST 240
                + +RSF+SSLSMD SV   D +SFHL G S      + R+       GSLKCGS 
Sbjct: 181 TPSVSNGSRSFVSSLSMDASVTDYDRNSFHLTGLSRGSDQQHTRKMC----SGSLKCGSR 240

Query: 241 GKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMR 300
            KCHCSKKRK RVKRSIKVPAISNK+ADIP D+YSWRKYGQKPIKGSPHPRGYYKCSS+R
Sbjct: 241 SKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSSVR 300

Query: 301 GCPARKHVERCLEDPSMLIVTYEGEHNHPK-MSTQSAHT 311
           GCPARKHVERC+++ SMLIVTYEGEHNH + +S+QSAHT
Sbjct: 301 GCPARKHVERCIDETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of Carg16860 vs. ExPASy Swiss-Prot
Match: Q32SG4 (Protein WRKY1 OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.3e-63
Identity = 170/406 (41.87%), Postives = 219/406 (53.94%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGEL--RKNLMVETGEAVFKLKKVVSLLNSG 60
           ME +EEANR A++SCH VL LL+ P     G+L   K L+  TGEAV K   + + L++ 
Sbjct: 1   MEGMEEANRTAVESCHRVLALLSNP----HGQLVPSKELVAATGEAVAKFGSLTAKLSNS 60

Query: 61  ------FGYAKVRRFK-------------------------------------------- 120
                  G+A+VR+ K                                            
Sbjct: 61  NGDGLLQGHARVRKVKKPLHIFDSNLFLESSAVAAAAAPAKTPSPSPILGLQLFPRYHQF 120

Query: 121 ---------NIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQ--NHKNSLKIHKQAA 180
                     IP   P++LLL+ P  G+ G  S     P ++M Q  +          A 
Sbjct: 121 EGSSSKDPVRIPTQFPKRLLLEKPTAGMEGSTS--QSPPIVQMVQPVSVAPPAGTPTPAL 180

Query: 181 PSLNFSFPQQQQ--------QQRKQAEMMFLRSN----------------SGMNMNFDAS 240
           P  +  F QQQQ        QQ K    M  RSN                 G+N+ FD+S
Sbjct: 181 PPAHLHFIQQQQSYQRFQLMQQMKIQSEMMKRSNLGDQGGSLSGGGGGGRKGVNLKFDSS 240

Query: 241 KCTLTMSSARSFISSLSMDGSVA--DGS----SFHLIGPSSTMSGD-----NKRRFSGRG 300
            C  T SS+RSF+SSLSM+GS+A  DGS     F L+  S T S        +RR +GR 
Sbjct: 241 NC--TASSSRSFLSSLSMEGSLASLDGSRTSRPFQLLSGSQTASTPELGLVQRRRCAGR- 300

Query: 301 DEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHP 309
           ++G+ +C +  +CHCSKKRK R++RSIKVPAISNK+ADIP+D++SWRKYGQKPIKGSPHP
Sbjct: 301 EDGTGRCATGSRCHCSKKRKLRIRRSIKVPAISNKVADIPADEFSWRKYGQKPIKGSPHP 360

BLAST of Carg16860 vs. ExPASy Swiss-Prot
Match: Q9SJA8 (Probable WRKY transcription factor 17 OS=Arabidopsis thaliana OX=3702 GN=WRKY17 PE=1 SV=2)

HSP 1 Score: 180.6 bits (457), Expect = 2.6e-44
Identity = 125/318 (39.31%), Postives = 176/318 (55.35%), Query Frame = 0

Query: 4   LEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFGYAK 63
           ++EA    + S   ++ +L+   P+E+      +   T   V K KKV+SLLN   G+A 
Sbjct: 17  IQEAASQGLKSMEHLIRVLSN-RPEERNVDCSEI---TDFTVSKFKKVISLLNRS-GHA- 76

Query: 64  VRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSLNFS 123
             RF+  P+  P    + PP       V V    P  ++      S     Q + +L+F+
Sbjct: 77  --RFRRGPVHSPPSSSVPPP-------VKVTTPAP-TQISAPAPVSFVQANQQSVTLDFT 136

Query: 124 FPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISS-LSMDGSVADGSSFH 183
            P     + K +E++            + +K + ++SS  SF+SS ++ DGSV+ GSS  
Sbjct: 137 RPSVFGAKTKSSEVV------------EFAKESFSVSSNSSFMSSAITGDGSVSKGSSIF 196

Query: 184 LI-GPSSTMSGDNKRRFSG-------------RGDEGSLKCGSTGKCHCSKKRKHRVKRS 243
           L   P+  ++   K   SG              G  G +     GKCHC K RK+R+KR+
Sbjct: 197 LAPAPAVPVTSSGKPPLSGLPYRKRCFEHDHSEGFSGKISGSGNGKCHCKKSRKNRMKRT 256

Query: 244 IKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPS 303
           ++VPA+S K+ADIP D+YSWRKYGQKPIKGSPHPRGYYKCS+ RGCPARKHVER L+D +
Sbjct: 257 VRVPAVSAKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSTFRGCPARKHVERALDDST 306

Query: 304 MLIVTYEGEHNHPKMSTQ 307
           MLIVTYEGEH H + + Q
Sbjct: 317 MLIVTYEGEHRHHQSTMQ 306

BLAST of Carg16860 vs. ExPASy TrEMBL
Match: A0A455PAJ4 (WRKY21 OS=Cucurbita moschata OX=3662 GN=LOC111463779 PE=2 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 4.8e-174
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG
Sbjct: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120
           YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL
Sbjct: 61  YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120

Query: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180
           NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS
Sbjct: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180

Query: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240
           FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD
Sbjct: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240

Query: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300
           IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH
Sbjct: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300

Query: 301 PKMSTQSAHT 311
           PKMSTQSAHT
Sbjct: 301 PKMSTQSAHT 310

BLAST of Carg16860 vs. ExPASy TrEMBL
Match: A0A6J1HUE3 (probable WRKY transcription factor 21 OS=Cucurbita maxima OX=3661 GN=LOC111466947 PE=4 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.1e-173
Identity = 307/310 (99.03%), Postives = 309/310 (99.68%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG
Sbjct: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120
           YAKVRRFKNIPLPLPQK LLDPPNNGLNGK+SVFLGNPDLEMDQNHKNSLKIHKQAAPSL
Sbjct: 61  YAKVRRFKNIPLPLPQKFLLDPPNNGLNGKISVFLGNPDLEMDQNHKNSLKIHKQAAPSL 120

Query: 121 NFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180
           NFSFPQQQQQQRKQAEMMF+RSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS
Sbjct: 121 NFSFPQQQQQQRKQAEMMFVRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVADGSS 180

Query: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240
           FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD
Sbjct: 181 FHLIGPSSTMSGDNKRRFSGRGDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLAD 240

Query: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300
           IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH
Sbjct: 241 IPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNH 300

Query: 301 PKMSTQSAHT 311
           PKMSTQSAHT
Sbjct: 301 PKMSTQSAHT 310

BLAST of Carg16860 vs. ExPASy TrEMBL
Match: A0A6H0CEQ4 (WRKY20 protein OS=Cucumis metulifer OX=61886 GN=WRKY20 PE=2 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.3e-130
Identity = 251/339 (74.04%), Postives = 271/339 (79.94%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+EEAN++AI+SCHGVLNLL QP P       KNLM+ET EAVFK KKV+SLLNS F 
Sbjct: 1   MEEVEEANKSAIESCHGVLNLLLQPPPHHHQHHFKNLMLETKEAVFKFKKVISLLNSDFN 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPPN--------------NGLNGKVSVFLGNPDLEMDQNH 120
           + + R F  IPLPLPQ  LLD PN               G N KVS+FLGNPDLE+ QN 
Sbjct: 61  HPRFRNFNKIPLPLPQNSLLDSPNYTLHPPNKNLFTFPPGFNSKVSIFLGNPDLELSQND 120

Query: 121 KNSLKIHKQAAPSLNFSFPQQQQQQ-----------RKQAEMMFLRSNSGMNMNFDASKC 180
           KNSL I KQ +PSLNFSFP   QQQ           ++QAEMMFLR+N+GMN+NFD S C
Sbjct: 121 KNSLHIPKQ-SPSLNFSFPHHHQQQQQSVLAHQKQMKQQAEMMFLRNNNGMNLNFDTSNC 180

Query: 181 TLTMSSARSFISSLSMDGSV-ADGSSFHLIGPS---STMSGDNKRRFSGRGDEGSLKCGS 240
           TLTMSSARSFISSLSMDGSV  D SSFHLIGPS   +T SGD+KR+FS RG+EGSLKCGS
Sbjct: 181 TLTMSSARSFISSLSMDGSVIGDRSSFHLIGPSTTTTTTSGDSKRKFSARGEEGSLKCGS 240

Query: 241 TGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM 300
           T KCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM
Sbjct: 241 TSKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSM 300

Query: 301 RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 311
           RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT
Sbjct: 301 RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 338

BLAST of Carg16860 vs. ExPASy TrEMBL
Match: A0A6J1CYE8 (probable WRKY transcription factor 21 OS=Momordica charantia OX=3673 GN=LOC111015354 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.2e-128
Identity = 255/334 (76.35%), Postives = 274/334 (82.04%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+EEANR A++SCHGVLNLLAQP+ Q Q    +NLMVETGEAVFK +KVVSLLNSG G
Sbjct: 1   MEEVEEANRVALESCHGVLNLLAQPADQIQ---LRNLMVETGEAVFKFRKVVSLLNSGLG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLD--------------PPNNGLNGKVSVFLGNPDLEMDQNH 120
           +A+VR+   I LPLPQ  LLD              P  +GLNGKVS FLG PDLE+  N 
Sbjct: 61  HARVRKLNKISLPLPQNTLLDYPIHHLPTQNQNLHPSQSGLNGKVSTFLGIPDLELGPND 120

Query: 121 KNSLKIHKQAAPSLNFSFPQQQQQQR---------KQAEMMFLR-SNSGMNMNFDASKCT 180
           KN L+I +Q APS    FPQ QQQQR         +Q+EMMFLR +NSGMN+NFD S CT
Sbjct: 121 KNFLQIPRQ-APSF---FPQHQQQQRILPQQKQIKQQSEMMFLRNNNSGMNLNFDTSNCT 180

Query: 181 LTMSSARSFISSLSMDGSVADGSSFHLIGPSSTMSGDNKRRFSGR-GDEGSLKCGSTGKC 240
           LTMSSARSFISSLSMDGSVADGSSFHLIGPS+  S DNKR+FSGR GDEGSLKCGSTGKC
Sbjct: 181 LTMSSARSFISSLSMDGSVADGSSFHLIGPST--SADNKRKFSGRGGDEGSLKCGSTGKC 240

Query: 241 HCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCP 300
           HCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCP
Sbjct: 241 HCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCP 300

Query: 301 ARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAH 310
           ARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAH
Sbjct: 301 ARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAH 325

BLAST of Carg16860 vs. ExPASy TrEMBL
Match: E7CEW1 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY9 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.9e-126
Identity = 250/349 (71.63%), Postives = 274/349 (78.51%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQ--PSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSG 60
           MEE+EEAN++AI+SCHGVLNLL Q  PSP       KNLM+ET EAVFK KKV+SLLNS 
Sbjct: 1   MEEVEEANKSAIESCHGVLNLLLQPPPSPHHHQHHFKNLMLETKEAVFKFKKVISLLNSD 60

Query: 61  FGYAKVRRFKNIPLPLPQKLLLDPPN--------------NGLNGKVSVFLGNPDLEMDQ 120
           F + + R F  IPLPLPQ  LLD PN               G N KVS+ LGNPDLE+ Q
Sbjct: 61  FSHPRFRNFNKIPLPLPQNSLLDSPNYTLHPPNKNLFNSPPGFNSKVSILLGNPDLELSQ 120

Query: 121 NHKNSLKIHKQAAPSLNFSFP--------QQQQQQRK-----------QAEMMFLRSNSG 180
           N KNSL I KQ +PSL+FSFP        QQQQQQ++           QAEMMFLR+N+G
Sbjct: 121 NDKNSLHIPKQ-SPSLSFSFPHHHHPQQQQQQQQQQQSLLAHQKQMKHQAEMMFLRNNNG 180

Query: 181 MNMNFDASKCTLTMSSARSFISSLSMDGSV-ADGSSFHLIGPS---STMSGDNKRRFSGR 240
           MN+NFD S CT+TMSSARSFISSLSMDGSV  D SSFHLIGPS   +T SG++KR+FS R
Sbjct: 181 MNLNFDTSNCTMTMSSARSFISSLSMDGSVIGDRSSFHLIGPSTTTTTTSGNSKRKFSAR 240

Query: 241 GDEGSLKCGSTGKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPH 300
           G+EGSLKCGST KCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPH
Sbjct: 241 GEEGSLKCGSTSKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPH 300

Query: 301 PRGYYKCSSMRGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 311
           PRGYYKCSS+RGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT
Sbjct: 301 PRGYYKCSSIRGCPARKHVERCLEDPSMLIVTYEGEHNHPKMSTQSAHT 348

BLAST of Carg16860 vs. TAIR 10
Match: AT2G30590.1 (WRKY DNA-binding protein 21 )

HSP 1 Score: 312.0 bits (798), Expect = 5.4e-85
Identity = 188/380 (49.47%), Postives = 237/380 (62.37%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+E  NRAA++SCH VLNLL +   Q+     KNL+ ET EAV + K+V SLL+S  G
Sbjct: 1   MEEIEGTNRAAVESCHRVLNLLHRSQQQDHVGFEKNLVSETREAVIRFKRVGSLLSSSVG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDP--------PNNG----------------LNGKVSVFLG 120
           +A+ RR K +   + Q LLLDP        P++                      S+ LG
Sbjct: 61  HARFRRAKKLQSHVSQSLLLDPCQQRTTEVPSSSSQKTPVLRSGFQELSLRQPSDSLTLG 120

Query: 121 NPDLEMDQNHKNS-LKIHKQAAPSLNFS--FPQQQQQQRK-------------------- 180
                ++ N K   L++++Q  P  N+   FP QQQQQ++                    
Sbjct: 121 TRSFSLNSNAKAPLLQLNQQTMPPSNYPTLFPVQQQQQQQQQQQQQEQQQQQQQQQQQFH 180

Query: 181 -----------------QAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISSLSMDGSVA 240
                            QAE+M  + N G++++FD S CT TMSS RSF+SSLS+DGSVA
Sbjct: 181 ERLQAHHLHQQQQLQKHQAELMLRKCNGGISLSFDNSSCTPTMSSTRSFVSSLSIDGSVA 240

Query: 241 D---GSSFHLIGPSST--MSGDNKRRFSGRGDE-GSLKCGSTGKCHCSKKRKHRVKRSIK 300
           +    +SFH   PSST   S  +KR+   +GDE GSLKCGS+ +CHC+KKRKHRV+RSI+
Sbjct: 241 NIEGKNSFHFGVPSSTDQNSLHSKRKCPLKGDEHGSLKCGSSSRCHCAKKRKHRVRRSIR 300

Query: 301 VPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPSML 311
           VPAISNK+ADIP DDYSWRKYGQKPIKGSP+PRGYYKCSSMRGCPARKHVERCLEDP+ML
Sbjct: 301 VPAISNKVADIPPDDYSWRKYGQKPIKGSPYPRGYYKCSSMRGCPARKHVERCLEDPAML 360

BLAST of Carg16860 vs. TAIR 10
Match: AT5G28650.1 (WRKY DNA-binding protein 74 )

HSP 1 Score: 287.0 bits (733), Expect = 1.9e-77
Identity = 178/338 (52.66%), Postives = 218/338 (64.50%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+E AN+AA++SCHGVLNLL+Q     Q    K++MVET EAV K K+V SLL+ G G
Sbjct: 1   MEEVEAANKAAVESCHGVLNLLSQ-----QTNDSKSIMVETREAVCKFKRVSSLLSRGLG 60

Query: 61  YAKVRRFKNIPLP-----LPQKLLLDPP---NNGLNGKVSVF---------LGNPDLEM- 120
             K+++  N         LPQ + L+ P   NN ++G + +           G P L + 
Sbjct: 61  QRKIKKLNNNNYKFSSSLLPQHMFLESPVCSNNAISGCIPILAPKPLQIVPAGPPPLMLF 120

Query: 121 DQNHKNSLKIHKQAAPSLNFSFPQQQQQQRKQAEMMFLRSNSGMNMNFDAS---KC--TL 180
           +QN        +   PS     P+  Q      + ++ RS SG+N+ FD S    C    
Sbjct: 121 NQNMCLDKSFLELKPPSSRAVDPKPYQFIHTHQQGVYSRSKSGLNLKFDGSIGASCYSPS 180

Query: 181 TMSSARSFISSLSMDGSVA--DGSSFHLIG--PSSTMSGDNKRRFSGRGDEGSLKCGSTG 240
             + +RSF+SSLSMDGSV   D +SFHLIG    S     + RR S     GSLKCGS  
Sbjct: 181 ISNGSRSFVSSLSMDGSVTDYDRNSFHLIGLPQGSDHISQHSRRTS---CSGSLKCGSKS 240

Query: 241 KCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG 300
           KCHCSKKRK RVKRSIKVPAISNK+ADIP D+YSWRKYGQKPIKGSPHPRGYYKCSS+RG
Sbjct: 241 KCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSSVRG 300

Query: 301 CPARKHVERCLEDPSMLIVTYEGEHNHPK-MSTQSAHT 311
           CPARKHVERC+E+ SMLIVTYEGEHNH + +S+QSAHT
Sbjct: 301 CPARKHVERCVEETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of Carg16860 vs. TAIR 10
Match: AT3G04670.1 (WRKY DNA-binding protein 39 )

HSP 1 Score: 286.6 bits (732), Expect = 2.4e-77
Identity = 173/339 (51.03%), Postives = 209/339 (61.65%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+E ANR+AI+SCHGVLNLL+Q +        K+L VETGE V K K+V SLL  G G
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLSQRTSDP-----KSLTVETGEVVSKFKRVASLLTRGLG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPP---NNGLNGKVSVFLGNPDLEM---DQNHKNSLKIHK 120
           + K R         PQ + L+ P    N L+G  +  L    L+M      +      H+
Sbjct: 61  HGKFRSTNKFRSSFPQHIFLESPICCGNDLSGDYTQVLAPEPLQMVPASAVYNEMEPKHQ 120

Query: 121 QAAPSLNFS----------------FPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTL- 180
              PSL  S                F    Q      ++ + RSNSG+N+ FD S  +  
Sbjct: 121 LGHPSLMLSHKMCVDKSFLELKPPPFRAPYQLIHNHQQIAYSRSNSGVNLKFDGSGSSCY 180

Query: 181 ---TMSSARSFISSLSMDGSVA--DGSSFHLIGPSSTMSGDNKRRFSGRGDEGSLKCGST 240
                + +RSF+SSLSMD SV   D +SFHL G S      + R+       GSLKCGS 
Sbjct: 181 TPSVSNGSRSFVSSLSMDASVTDYDRNSFHLTGLSRGSDQQHTRKMC----SGSLKCGSR 240

Query: 241 GKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMR 300
            KCHCSKKRK RVKRSIKVPAISNK+ADIP D+YSWRKYGQKPIKGSPHPRGYYKCSS+R
Sbjct: 241 SKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSSVR 300

Query: 301 GCPARKHVERCLEDPSMLIVTYEGEHNHPK-MSTQSAHT 311
           GCPARKHVERC+++ SMLIVTYEGEHNH + +S+QSAHT
Sbjct: 301 GCPARKHVERCIDETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of Carg16860 vs. TAIR 10
Match: AT3G04670.2 (WRKY DNA-binding protein 39 )

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-53
Identity = 137/295 (46.44%), Postives = 166/295 (56.27%), Query Frame = 0

Query: 1   MEELEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFG 60
           MEE+E ANR+AI+SCHGVLNLL+Q +        K+L VETGE V K K+V SLL  G G
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLSQRTSDP-----KSLTVETGEVVSKFKRVASLLTRGLG 60

Query: 61  YAKVRRFKNIPLPLPQKLLLDPP---NNGLNGKVSVFLGNPDLEM---DQNHKNSLKIHK 120
           + K R         PQ + L+ P    N L+G  +  L    L+M      +      H+
Sbjct: 61  HGKFRSTNKFRSSFPQHIFLESPICCGNDLSGDYTQVLAPEPLQMVPASAVYNEMEPKHQ 120

Query: 121 QAAPSLNFS----------------FPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTL- 180
              PSL  S                F    Q      ++ + RSNSG+N+ FD S  +  
Sbjct: 121 LGHPSLMLSHKMCVDKSFLELKPPPFRAPYQLIHNHQQIAYSRSNSGVNLKFDGSGSSCY 180

Query: 181 ---TMSSARSFISSLSMDGSVA--DGSSFHLIGPSSTMSGDNKRRFSGRGDEGSLKCGST 240
                + +RSF+SSLSMD SV   D +SFHL G S      + R+       GSLKCGS 
Sbjct: 181 TPSVSNGSRSFVSSLSMDASVTDYDRNSFHLTGLSRGSDQQHTRKMC----SGSLKCGSR 240

Query: 241 GKCHCSKKRKHRVKRSIKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYK 268
            KCHCSKKRK RVKRSIKVPAISNK+ADIP D+YSWRKYGQKPIKGSPHPR  YK
Sbjct: 241 SKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRYIYK 286

BLAST of Carg16860 vs. TAIR 10
Match: AT2G24570.1 (WRKY DNA-binding protein 17 )

HSP 1 Score: 180.6 bits (457), Expect = 1.9e-45
Identity = 125/318 (39.31%), Postives = 176/318 (55.35%), Query Frame = 0

Query: 4   LEEANRAAIDSCHGVLNLLAQPSPQEQGELRKNLMVETGEAVFKLKKVVSLLNSGFGYAK 63
           ++EA    + S   ++ +L+   P+E+      +   T   V K KKV+SLLN   G+A 
Sbjct: 17  IQEAASQGLKSMEHLIRVLSN-RPEERNVDCSEI---TDFTVSKFKKVISLLNRS-GHA- 76

Query: 64  VRRFKNIPLPLPQKLLLDPPNNGLNGKVSVFLGNPDLEMDQNHKNSLKIHKQAAPSLNFS 123
             RF+  P+  P    + PP       V V    P  ++      S     Q + +L+F+
Sbjct: 77  --RFRRGPVHSPPSSSVPPP-------VKVTTPAP-TQISAPAPVSFVQANQQSVTLDFT 136

Query: 124 FPQQQQQQRKQAEMMFLRSNSGMNMNFDASKCTLTMSSARSFISS-LSMDGSVADGSSFH 183
            P     + K +E++            + +K + ++SS  SF+SS ++ DGSV+ GSS  
Sbjct: 137 RPSVFGAKTKSSEVV------------EFAKESFSVSSNSSFMSSAITGDGSVSKGSSIF 196

Query: 184 LI-GPSSTMSGDNKRRFSG-------------RGDEGSLKCGSTGKCHCSKKRKHRVKRS 243
           L   P+  ++   K   SG              G  G +     GKCHC K RK+R+KR+
Sbjct: 197 LAPAPAVPVTSSGKPPLSGLPYRKRCFEHDHSEGFSGKISGSGNGKCHCKKSRKNRMKRT 256

Query: 244 IKVPAISNKLADIPSDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEDPS 303
           ++VPA+S K+ADIP D+YSWRKYGQKPIKGSPHPRGYYKCS+ RGCPARKHVER L+D +
Sbjct: 257 VRVPAVSAKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSTFRGCPARKHVERALDDST 306

Query: 304 MLIVTYEGEHNHPKMSTQ 307
           MLIVTYEGEH H + + Q
Sbjct: 317 MLIVTYEGEHRHHQSTMQ 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022963451.19.9e-174100.00probable WRKY transcription factor 21 [Cucurbita moschata] >AZZ86683.1 WRKY21 [C... [more]
XP_022967410.18.4e-17399.03probable WRKY transcription factor 21 [Cucurbita maxima][more]
XP_023554095.11.4e-17299.03probable WRKY transcription factor 21 [Cucurbita pepo subsp. pepo][more]
XP_038886893.12.0e-13478.47probable WRKY transcription factor 21 isoform X1 [Benincasa hispida][more]
QIS68546.18.8e-13074.04WRKY20 protein [Cucumis metulifer][more]
Match NameE-valueIdentityDescription
O043367.6e-8449.47Probable WRKY transcription factor 21 OS=Arabidopsis thaliana OX=3702 GN=WRKY21 ... [more]
Q93WU62.6e-7652.66Probable WRKY transcription factor 74 OS=Arabidopsis thaliana OX=3702 GN=WRKY74 ... [more]
Q9SR073.4e-7651.03Probable WRKY transcription factor 39 OS=Arabidopsis thaliana OX=3702 GN=WRKY39 ... [more]
Q32SG43.3e-6341.87Protein WRKY1 OS=Zea mays OX=4577 PE=1 SV=1[more]
Q9SJA82.6e-4439.31Probable WRKY transcription factor 17 OS=Arabidopsis thaliana OX=3702 GN=WRKY17 ... [more]
Match NameE-valueIdentityDescription
A0A455PAJ44.8e-174100.00WRKY21 OS=Cucurbita moschata OX=3662 GN=LOC111463779 PE=2 SV=1[more]
A0A6J1HUE34.1e-17399.03probable WRKY transcription factor 21 OS=Cucurbita maxima OX=3661 GN=LOC11146694... [more]
A0A6H0CEQ44.3e-13074.04WRKY20 protein OS=Cucumis metulifer OX=61886 GN=WRKY20 PE=2 SV=1[more]
A0A6J1CYE85.2e-12876.35probable WRKY transcription factor 21 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
E7CEW14.9e-12671.63WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G30590.15.4e-8549.47WRKY DNA-binding protein 21 [more]
AT5G28650.11.9e-7752.66WRKY DNA-binding protein 74 [more]
AT3G04670.12.4e-7751.03WRKY DNA-binding protein 39 [more]
AT3G04670.21.4e-5346.44WRKY DNA-binding protein 39 [more]
AT2G24570.11.9e-4539.31WRKY DNA-binding protein 17 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 242..302
e-value: 9.3E-40
score: 148.1
IPR003657WRKY domainPFAMPF03106WRKYcoord: 244..301
e-value: 9.7E-27
score: 93.0
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 237..303
score: 30.985598
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 226..302
e-value: 4.0E-32
score: 111.8
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 240..302
IPR018872Zn-cluster domainPFAMPF10533Plant_zn_clustcoord: 203..240
e-value: 3.8E-17
score: 62.1
NoneNo IPR availablePANTHERPTHR31282:SF172WRKY TRANSCRIPTION FACTOR 74-RELATEDcoord: 3..310
IPR044810WRKY transcription factor, plantPANTHERPTHR31282WRKY TRANSCRIPTION FACTOR 21-RELATEDcoord: 3..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16860-RACarg16860-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding