Carg16855 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16855
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMagnesium transporter
LocationCarg_Chr19: 324073 .. 328553 (+)
RNA-Seq ExpressionCarg16855
SyntenyCarg16855
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTTCCTCTTTCTGAAACTTTCTGCCCCAACTTTCTCATAACTGAACCCATTATTCCCCCTCACATTTTCTAATCGGAATCTGTTTTTCATGCCGTTGTTCAACTCCAATTCGTCGTTGAGTTGAATCATTCACGCGCCAAATCACCGCAGATTTCCAGATTCTTTTTCTTCGACGTGTTTTCACTGAGCATTGGTTTCCGAAGAAGAGCAATGGTTTTGGAATCGATATTGTTTTAGAGATGGCTGGAGATGGATTTGGATTCCCTGTGGAATCTCAGGCGGTTTCGGTGAAGAAGAAGACGGTGGCGACCACCACGACCACGACGAAGAAGAAGAATACTGTAGTTTCCAGGAGCTGGGTTTCGTTGGACCATGAAGGAAGGACTACGGTTTTGGACGTGGATAAGTATGCTATTATGCAGAGAGTTCAGATTAATGCGAGAGATCTTCGGCTTCTTGATCCTCTGTTGTCGTATCCTTCCACTATCTTGGGGAGAGAAAGCGTGATTGTTCTCAATTTGGAGGTAAACTTACTCGAGATTGATTCTTCTTGTGAAATTCTGCTCATCTAACTTTCTTCAAATACATTTGTAATTTTTTGGTGTTATTATGCTGATTTTGATTCTGGTATCGCTCTGGGATTTGGTCCAGCACATCAAGTCAATAATCACTGCAGACGAGGTAATAATTTGTTTATCCTTTTCTTGATTAATTAACTGCTCATTAATTAATCTTTCCTTAAACTGATTATTTTGTTGGGTTTATTTAGGCTAATTCTTGTTTTACTCTGTTTATCTTATGATGGATTCTTTTTCCAAACCCCTTTTTGGGGTTTTATAGTCCCAAAAAGGTTTCAAGTTGACTATTATAATGGAAGTATTTATGTGCTTAGACTGTTCAAAGGATGAATCAAATTGACAAACCAAGACGTATGAGAGGAGAGCTAAAAGAAGAACTCAAAACTCTTTTTCTTGGTTCAATTTTTACTATTTGTGTTTATAGGTGCTGCTTAGAGACCCAATGGATGAAAATGTAGTTCCAATTGTTGAAGAATTGCAGAGACGGTTGCCTTCCACAAATTCCCTGTACCAAGGACAAGGGGAAGAAGAAGAGCCTTCCACCACCCAAAATGAGTTGGCTGAAAACGGTACATCTTGGTCAAGTATTCTTTCATGGGTTTTAACCCTTGTATTCAATGATTCCATATTTGGAAAGATAGTTATCATTGCTTGAAAATATTGTACTGTTTTAAATAGTTGTTTGAAATTTCTTTCTTCCCTTTTAAGAGTTTTGTCATTCAATGCAGAATTTCCATTTGAGTTTCGTGCCTTAGAGGTCGCATTAGAAGCAATTTGTAGTTTTCTTGATGCACGCACAAGAGAACTGGAGACTGATACGTATCCTGCTTTAGATGAGCTGACCTCCAAGGTGAAGCTTGTTTAATTCTGCTCCCTTAGTACATATCCATTTAGTTGATCCATTCTTGAAATTTCAGCTTTTTACTGAAACAGAGATAAACTTTCACAAATCTATAATACACTGCCTTCTTTATTTTTTGAGTTTTAAAAAAGCGTTGGATGTTCTTCTAGAATTTCTTTGTCTTTTTTAACTTCCTTATTCATCTCAAGTTCTCTAGGCTTGATTATTAATTGAAAGCATTTAGTTTATTAGTGGATTTGATGAACAAATGTCCAGAAAAAATGTATTTTCACTTTTCAGTTAGATTTATTTCCATTAAGAGAAACAGTACTTTTTCATCAAGATAATTATCTTCAAGGGACTTTTATGGGTATAATCTTCCATACCATCTTATGTTTCTCTAGTAAAGACTTGAAAGTTGACAATGAAATACTAATGGAAGGTTGATATCCTTCTATTAGAAAGCCAATGTGGTAAAATAATAATAATAATAATAATAGTAATATATGATTGTATTCTTTCAATTAGAGATTCCATTTGAGTTTTATAATTGTGGTTGGTACATGGGAAAGGGACGAGTCATATTTAATCAAATTCAATGTTAACCTTTGGTTACAAACCTCATCAAAGTTATACTGCATCTATCATTAAGTCCTCAAAAAAGCTAACTAAAAAGTTTTCGATTATGCTTTTGGGTGAATAGTTGTGTTGCTAACCATGTTCTTGTACTATTCAAGCTGTATCATAAAAAAGATTTTCTTCAAACGAACAGATACAAATGTTTACTACCCCCATCTAGTGTTTGATTTGAATTAGGAACCGCATTCATGTCTTTACATGATTGCCTATTAGTGGGCATCAGTCAAAGAATTCTGAATTTATATGCAGATTAGCAGTCGTAACTTGGATCGAGTGCGCAAGCTGAAGAGTGCAATGACAAGGTTGACAAATCGGGTGCAAAAGGTAGCTCGTTTTCTGTGCCATCTTGAGTATGAGGCTATGAGCCCTTCTCTGTTTTCACTCTCTTGGGTCTTCAACTAAATATTTACAAGCTAACAGCAAAGCGAAGTTGCATTGCTCGGCTCCGAGTTTGATACTACTCTAGGATGGTATATAGGTTGAGCTTCATGGTACTGGCTACTTTCAATTCTCAGGAGAAGTAGACAATAAATCATGAAGCAAGTAGTTTTTTATTACACGCTCATTCTTTTATATCTCGGGTTCCATATGCTGGATCTTTAATTACAACAGTCGGTCAACAATTCTGCTGACCCCGGCGGCTTATACGATTTTACCCCGAGTTTGCCCCAAAGTCTAGGAGTCTTTTTGCACTGACGATTGTGGTGATTAGGTCTTGAACCTTTTGTTGGTATGCCCCTGCTAGCAAAGCTATACTGGTTCGAGTTTACTCTGTAAACCCCAGCTTGCAATTCACCTTTCTCCTTGTGAATATTATCGGACTGCCAAATTTTTATACGTTCCTGTTGCTGCTCAATGCTCATTTAATCTTAGTTTGGTAGAGCCAAAATGAGCTGGAAAGCAACAAAATAAATGATCATTCTTGACATAAGTATGAGCCAACTAGTCCCCTCAAGATCCCTCAACGATTTGCTTGTCAAAAGAACAGAAAAAGTAAAGATTTGTAGTAGTAGCCAACTGATGTTTTTTTCTGATGCAATTTCTTTATCTTCATGGCCATGATATTACACCATTCTCATTCTAGGTTAGGGATGAACTTGAACAACTTCTGGATGACGACGACGACATGGCGGAGCTTTACTTGTCACGAAAAGTAGCTGGCACCCCTGAAAGCGGCTCTGGGACTCCAATTTGGTTTCTTGCTTCTCCTAAGGACTATTCAAAGATGTCTAAAACAAGTAGAGCAAGTGCAATTACTTTTCGAGGAGAGAATGATGTTGAGGAACTAGAAATGTTACTCGAGGTATGATCAATGTCTTTAAGAACAGAATATAACATCTAATTCACAGAGTTTCAAGTAAATTAAGATCTAATGGAGCTTAATTATGAACTCTCTGCAGGCTTATTTTATGCAGATTGAAGGTACATTAAACAAACTAATCACGGTATGAACGAATTGCTTTCTGTTCATATCTGATATTTTGTATATGTAACTTTCTGATTATGTGGTATTTTTGTTCATATATTTTGAAATCGTCTTCCCTTACCAGTTGCGGGAATACATCGATGACACAGAGGATTACATCAATATTCAGGTAATCCTTCAACATCCTTGACTATCTAATACTCAATCATGTCATTGATATCATTTTCTTGTAACTTTTACTCCTCAATATTCTTAGCAATGTTGAAAACTACAATATACTTATGTTTCTGTATCAAATGCCCTGTTCATCTGCTGCAGCTTGACAATCACAGGAATCAACTGATTCAGGTACATTTTTCTCTTTGGCTTACAATTGTGGATCTATTGGCAATTGAAATGGCCATTGATTTCTGGCCACAAAAAATTCATCTGCAACTACTATATCATGTTTTTTCTTTTAGTTCTCAGAAAAGTAATGTTCTATGTGGCAGCTTGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCGATATTTGGAATGAATATCCCATATACCTGGAAGGAAGATCATGAACATGTATTCAAATGGGTAAGTGTTTTTAGTAAAATTGAATGGAAAATCATACCAAGAATACGATGCTTGTTGATCTTCTCCTTCCCATGACTTTAGGTGGTCATCCTGACAGGAATAGTTTGTGCAACCATTTATGTGTCACTAAGCTCGTATGCACGATCCAAAGGTCTTGTTGGATCTTGAACGTTACGCTCGTAGTGTTTCCACGGTTTTGAGATATTATGAACTACTCGAATACGAGACGATTCTTGGAGTCTGTTGGGGAGGATTTGGAAGTTGTAACACTGTGATATGTTACAAGGAAGACAATGATAGCTTATGCTTGAGTCAGATATATTTTCCAGGCGTGTAATGTTTTTGATATTTTACCTTTTTTTTTTTGTTATCATGTACAATTAT

mRNA sequence

CATTTCCTCTTTCTGAAACTTTCTGCCCCAACTTTCTCATAACTGAACCCATTATTCCCCCTCACATTTTCTAATCGGAATCTGTTTTTCATGCCGTTGTTCAACTCCAATTCGTCGTTGAGTTGAATCATTCACGCGCCAAATCACCGCAGATTTCCAGATTCTTTTTCTTCGACGTGTTTTCACTGAGCATTGGTTTCCGAAGAAGAGCAATGGTTTTGGAATCGATATTGTTTTAGAGATGGCTGGAGATGGATTTGGATTCCCTGTGGAATCTCAGGCGGTTTCGGTGAAGAAGAAGACGGTGGCGACCACCACGACCACGACGAAGAAGAAGAATACTGTAGTTTCCAGGAGCTGGGTTTCGTTGGACCATGAAGGAAGGACTACGGTTTTGGACGTGGATAAGTATGCTATTATGCAGAGAGTTCAGATTAATGCGAGAGATCTTCGGCTTCTTGATCCTCTGTTGTCGTATCCTTCCACTATCTTGGGGAGAGAAAGCGTGATTGTTCTCAATTTGGAGCACATCAAGTCAATAATCACTGCAGACGAGGTGCTGCTTAGAGACCCAATGGATGAAAATGTAGTTCCAATTGTTGAAGAATTGCAGAGACGGTTGCCTTCCACAAATTCCCTGTACCAAGGACAAGGGGAAGAAGAAGAGCCTTCCACCACCCAAAATGAGTTGGCTGAAAACGAATTTCCATTTGAGTTTCGTGCCTTAGAGGTCGCATTAGAAGCAATTTGTAGTTTTCTTGATGCACGCACAAGAGAACTGGAGACTGATACGTATCCTGCTTTAGATGAGCTGACCTCCAAGATTAGCAGTCGTAACTTGGATCGAGTGCGCAAGCTGAAGAGTGCAATGACAAGGTTGACAAATCGGGTGCAAAAGGTTAGGGATGAACTTGAACAACTTCTGGATGACGACGACGACATGGCGGAGCTTTACTTGTCACGAAAAGTAGCTGGCACCCCTGAAAGCGGCTCTGGGACTCCAATTTGGTTTCTTGCTTCTCCTAAGGACTATTCAAAGATGTCTAAAACAAGTAGAGCAAGTGCAATTACTTTTCGAGGAGAGAATGATGTTGAGGAACTAGAAATGTTACTCGAGGCTTATTTTATGCAGATTGAAGGTACATTAAACAAACTAATCACGTTGCGGGAATACATCGATGACACAGAGGATTACATCAATATTCAGCTTGACAATCACAGGAATCAACTGATTCAGCTTGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCGATATTTGGAATGAATATCCCATATACCTGGAAGGAAGATCATGAACATGTATTCAAATGGGTGGTCATCCTGACAGGAATAGTTTGTGCAACCATTTATGTGTCACTAAGCTCGTATGCACGATCCAAAGGTCTTGTTGGATCTTGAACGTTACGCTCGTAGTGTTTCCACGGTTTTGAGATATTATGAACTACTCGAATACGAGACGATTCTTGGAGTCTGTTGGGGAGGATTTGGAAGTTGTAACACTGTGATATGTTACAAGGAAGACAATGATAGCTTATGCTTGAGTCAGATATATTTTCCAGGCGTGTAATGTTTTTGATATTTTACCTTTTTTTTTTTGTTATCATGTACAATTAT

Coding sequence (CDS)

ATGGCTGGAGATGGATTTGGATTCCCTGTGGAATCTCAGGCGGTTTCGGTGAAGAAGAAGACGGTGGCGACCACCACGACCACGACGAAGAAGAAGAATACTGTAGTTTCCAGGAGCTGGGTTTCGTTGGACCATGAAGGAAGGACTACGGTTTTGGACGTGGATAAGTATGCTATTATGCAGAGAGTTCAGATTAATGCGAGAGATCTTCGGCTTCTTGATCCTCTGTTGTCGTATCCTTCCACTATCTTGGGGAGAGAAAGCGTGATTGTTCTCAATTTGGAGCACATCAAGTCAATAATCACTGCAGACGAGGTGCTGCTTAGAGACCCAATGGATGAAAATGTAGTTCCAATTGTTGAAGAATTGCAGAGACGGTTGCCTTCCACAAATTCCCTGTACCAAGGACAAGGGGAAGAAGAAGAGCCTTCCACCACCCAAAATGAGTTGGCTGAAAACGAATTTCCATTTGAGTTTCGTGCCTTAGAGGTCGCATTAGAAGCAATTTGTAGTTTTCTTGATGCACGCACAAGAGAACTGGAGACTGATACGTATCCTGCTTTAGATGAGCTGACCTCCAAGATTAGCAGTCGTAACTTGGATCGAGTGCGCAAGCTGAAGAGTGCAATGACAAGGTTGACAAATCGGGTGCAAAAGGTTAGGGATGAACTTGAACAACTTCTGGATGACGACGACGACATGGCGGAGCTTTACTTGTCACGAAAAGTAGCTGGCACCCCTGAAAGCGGCTCTGGGACTCCAATTTGGTTTCTTGCTTCTCCTAAGGACTATTCAAAGATGTCTAAAACAAGTAGAGCAAGTGCAATTACTTTTCGAGGAGAGAATGATGTTGAGGAACTAGAAATGTTACTCGAGGCTTATTTTATGCAGATTGAAGGTACATTAAACAAACTAATCACGTTGCGGGAATACATCGATGACACAGAGGATTACATCAATATTCAGCTTGACAATCACAGGAATCAACTGATTCAGCTTGAACTCTTTTTGAGCTCTGGGACTGTTTGTTTGTCTATTTATTCTTTAGTGACTGCGATATTTGGAATGAATATCCCATATACCTGGAAGGAAGATCATGAACATGTATTCAAATGGGTGGTCATCCTGACAGGAATAGTTTGTGCAACCATTTATGTGTCACTAAGCTCGTATGCACGATCCAAAGGTCTTGTTGGATCTTGA

Protein sequence

MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIMQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS
Homology
BLAST of Carg16855 vs. NCBI nr
Match: KAG6571485.1 (Magnesium transporter MRS2-7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011247.1 Magnesium transporter MRS2-7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 764.6 bits (1973), Expect = 4.2e-217
Identity = 400/400 (100.00%), Postives = 400/400 (100.00%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of Carg16855 vs. NCBI nr
Match: XP_022963506.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 758.8 bits (1958), Expect = 2.3e-215
Identity = 397/400 (99.25%), Postives = 398/400 (99.50%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           MAGDGFGFPVESQAVSVKKKT ATTTTTTKKKNTVVSRSWVSLDHEGR+TVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTAATTTTTTKKKNTVVSRSWVSLDHEGRSTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPS TQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSHTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of Carg16855 vs. NCBI nr
Match: XP_023554091.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.1 bits (1951), Expect = 1.5e-214
Identity = 397/401 (99.00%), Postives = 398/401 (99.25%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKT-VATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAI 60
           MAGDGFGFPVESQAVSVKKKT   TTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYA+
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTATTTTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAL 60

Query: 61  MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120
           MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI
Sbjct: 61  MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120

Query: 121 VEELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRE 180
           VEELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRE
Sbjct: 121 VEELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRE 180

Query: 181 LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYL 240
           LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYL
Sbjct: 181 LETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYL 240

Query: 241 SRKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIE 300
           SRKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIE
Sbjct: 241 SRKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIE 300

Query: 301 GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP 360
           GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP
Sbjct: 301 GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP 360

Query: 361 YTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           YTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS
Sbjct: 361 YTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401

BLAST of Carg16855 vs. NCBI nr
Match: XP_022967532.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 749.2 bits (1933), Expect = 1.8e-212
Identity = 394/400 (98.50%), Postives = 394/400 (98.50%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           MAGDGFGFPVESQ VSVKKK   T TTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQVVSVKKK---TATTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETD YPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDAYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 397

BLAST of Carg16855 vs. NCBI nr
Match: XP_038888713.1 (magnesium transporter MRS2-I-like [Benincasa hispida])

HSP 1 Score: 713.0 bits (1839), Expect = 1.5e-201
Identity = 378/400 (94.50%), Postives = 382/400 (95.50%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           MAG+GF  PVESQ  SVKKK     TTT KKKNT +SRSWVSLD EGR+T+LDVDKYAIM
Sbjct: 1   MAGNGFVIPVESQVGSVKKKK-KKQTTTKKKKNTAISRSWVSLDREGRSTILDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICS LDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSCLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISS NLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSLNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAIT RGENDVEELEMLLEAYFMQIE 
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITTRGENDVEELEMLLEAYFMQIES 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVILTGIVCATIYVSLSSYAR KGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARYKGLVGS 399

BLAST of Carg16855 vs. ExPASy Swiss-Prot
Match: Q10D38 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I PE=2 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 4.2e-143
Identity = 275/373 (73.73%), Postives = 308/373 (82.57%), Query Frame = 0

Query: 32  KNTVVSRSWVSLDHEGRTTVLDVDKYAIMQRVQINARDLRLLDPLLSYPSTILGRESVIV 91
           K    SRSW+  D  G   VLD DKYAIM RV INARDLR+LDPLLSYPSTILGRE  IV
Sbjct: 23  KKRGASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRILDPLLSYPSTILGRERAIV 82

Query: 92  LNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRL-PSTNSLYQGQGEEEEPSTTQNEL 151
           LNLEHIK+IITA+EVLLRDP+D+NV+P+VEEL+RRL PS+ + +  +G EE+        
Sbjct: 83  LNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSATQHDVEGAEED-------- 142

Query: 152 AENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAM 211
              E PFEFRALEV LEAICSFL ART ELE+  YPALDELTSKISSRNLDRVRKLKS M
Sbjct: 143 ---ESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELTSKISSRNLDRVRKLKSGM 202

Query: 212 TRLTNRVQKVRDELEQLLDDDDDMAELYLSRKVAG--TPESGSGTPIWFLASPKDYSKMS 271
           TRL  RVQKVRDELEQLLDDDDDMA+LYLSRK+AG  +P SGSG P WF ASP   SK+S
Sbjct: 203 TRLNARVQKVRDELEQLLDDDDDMADLYLSRKLAGAASPVSGSGGPNWFPASPTIGSKIS 262

Query: 272 KTSRASAITFRG-ENDVEELEMLLEAYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHR 331
           + SRASA T  G ENDVEELEMLLEAYFMQI+GTLNKL TLREYIDDTEDYINIQLDNHR
Sbjct: 263 RASRASAPTIHGNENDVEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDNHR 322

Query: 332 NQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVS 391
           NQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTW ++H +VFKWVV+++G+ CA ++VS
Sbjct: 323 NQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYVFKWVVLVSGLFCAFMFVS 382

Query: 392 LSSYARSKGLVGS 401
           + +YAR KGLVGS
Sbjct: 383 IVAYARHKGLVGS 384

BLAST of Carg16855 vs. ExPASy Swiss-Prot
Match: B8AJT9 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.6e-142
Identity = 280/391 (71.61%), Postives = 315/391 (80.56%), Query Frame = 0

Query: 14  AVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIMQRVQINARDLRLL 73
           AV V  +  A      KK+    SRSW+  D  G   VLD DKYAIM RV INARDLR+L
Sbjct: 4   AVVVAGEAAAAAGAGGKKRG--ASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRIL 63

Query: 74  DPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIVEELQRRL-PSTNS 133
           DPLLSYPSTILGRE  IVLNLEHIK+IITA+EVLLRDP+D+NV+P+VEEL+RRL PS+ +
Sbjct: 64  DPLLSYPSTILGRERAIVLNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSAT 123

Query: 134 LYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTRELETDTYPALDELT 193
            +  +G EE+           E PFEFRALEV LEAICSFL ART ELE+  YPALDELT
Sbjct: 124 QHDVEGAEED-----------ESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELT 183

Query: 194 SKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLSRKVAG--TPESG 253
           SKISSRNLDRVRKLKS MTRL  RVQKVRDELEQLLDDDDDMA+LYLSRK+AG  +P SG
Sbjct: 184 SKISSRNLDRVRKLKSGMTRLNARVQKVRDELEQLLDDDDDMADLYLSRKLAGAASPVSG 243

Query: 254 SGTPIWFLASPKDYSKMSKTSRASAITFRG-ENDVEELEMLLEAYFMQIEGTLNKLITLR 313
           SG P WF ASP   SK+S+ SRASA T  G ENDVEELEMLLEAYFMQI+GTLNKL TLR
Sbjct: 244 SGGPNWFPASPTIGSKISRASRASAPTIHGNENDVEELEMLLEAYFMQIDGTLNKLTTLR 303

Query: 314 EYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHV 373
           EYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTW ++H +V
Sbjct: 304 EYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYV 363

Query: 374 FKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           FKWVV+++G+ CA ++VS+ +YAR KGLVGS
Sbjct: 364 FKWVVLVSGLFCAFMFVSIVAYARHKGLVGS 381

BLAST of Carg16855 vs. ExPASy Swiss-Prot
Match: Q9FLG2 (Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 8.2e-139
Identity = 279/408 (68.38%), Postives = 318/408 (77.94%), Query Frame = 0

Query: 1   MAGDGFGFPVE-SQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAI 60
           MA +G+  P + S  V+VKKKT                 SW  +D  G++  LDVDKY I
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKT--------------PQASWALIDATGQSEPLDVDKYEI 60

Query: 61  MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120
           M RVQI+ARDLR+LDP LSYPSTILGRE  IVLNLEHIK+IIT++EVLLRDP DENV+P+
Sbjct: 61  MHRVQIHARDLRILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPV 120

Query: 121 VEELQRRLPSTNSLYQ-GQGEEEEPSTTQNE---LAENEFPFEFRALEVALEAICSFLDA 180
           VEEL+RRLP  N+ +  GQG+ +E +  QN+     E+E PFEFRALEVALEAICSFL A
Sbjct: 121 VEELRRRLPVGNASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAA 180

Query: 181 RTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMA 240
           RT ELET  YPALDELTSKISSRNLDRVRKLKSAMTRLT RVQKVRDELEQLLDDDDDMA
Sbjct: 181 RTAELETAAYPALDELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMA 240

Query: 241 ELYLSRKV--AGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRG-ENDVEELEMLLE 300
           +LYLSRK+  A +P S  G P W+  SP   SK+S+ SRAS  T  G ENDVEELEMLLE
Sbjct: 241 DLYLSRKLSSASSPISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLE 300

Query: 301 AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTA 360
           AYFMQI+ TLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  
Sbjct: 301 AYFMQIDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAG 360

Query: 361 IFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           IFGMNIPYTW + H ++FK+VV LTG +C  ++V + SYAR KGLVGS
Sbjct: 361 IFGMNIPYTWNDGHGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of Carg16855 vs. ExPASy Swiss-Prot
Match: Q304A0 (Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 5.1e-133
Identity = 264/406 (65.02%), Postives = 305/406 (75.12%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           M+ DG   PV+S AV            T K+K + +SRSW+S+D  G+ TVLDVDK+ IM
Sbjct: 1   MSPDGELVPVDSSAV-----------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
            RVQI+ARDLR+LDP L YPS ILGRE  IVLNLEHIK+IITA+EVL+RD  DENV+P++
Sbjct: 61  HRVQIHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVL 120

Query: 121 EELQRRLPSTNSLY--QGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTR 180
           EE QRRLP  N  +   G G+  E         E+E PFEFRALEVALEAICSFL ART 
Sbjct: 121 EEFQRRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTT 180

Query: 181 ELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELY 240
           ELE   YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LY
Sbjct: 181 ELEKFAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLY 240

Query: 241 LSRKVAGTPE--SGSGTPIWFLASPKDYSKMSKTSRASAITFRG--ENDVEELEMLLEAY 300
           L+RK+ G     S S  PIW+  SP   S +S+ SR S +T RG  E DVEELEMLLEAY
Sbjct: 241 LTRKLVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAY 300

Query: 301 FMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIF 360
           FMQI+ TLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IF
Sbjct: 301 FMQIDSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIF 360

Query: 361 GMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           GMNIP TW  DH ++FKWVV LTG  C  ++V + SYAR +GL+GS
Sbjct: 361 GMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of Carg16855 vs. ExPASy Swiss-Prot
Match: P0CZ21 (Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.9e-109
Identity = 222/370 (60.00%), Postives = 279/370 (75.41%), Query Frame = 0

Query: 37  SRSWVSLDHEGRTTVLDVDKYAIMQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEH 96
           S SW+S+D  G+ TVLDVDKY IM RVQI+ARDLR+LDP L YPS ILGRE  IVLNLEH
Sbjct: 18  SWSWISIDATGKKTVLDVDKYVIMHRVQIHARDLRILDPNLFYPSAILGRERAIVLNLEH 77

Query: 97  IKSIITADEVLLRDPMDENVVPIVEELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFP 156
           IK+IITA EVL++D  DEN++P +EE Q RL   N  + GQ + +        + E+E  
Sbjct: 78  IKAIITAKEVLIQDSSDENLIPTLEEFQTRLSVGNKAHGGQLDGD-------VVEEDESA 137

Query: 157 FEFRALEVALEAICSFLDARTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNR 216
           FEFRALEVALEAICSFL ART ELE   YPALDELT K++SRNL RV KLKS+MTRLT +
Sbjct: 138 FEFRALEVALEAICSFLAARTIELEKSAYPALDELTLKLTSRNLLRVCKLKSSMTRLTAQ 197

Query: 217 VQKVRDELEQLLDDDDDMAELYLSRKVAG--TPESGSGTPI-WFLASPKDYSKMS--KTS 276
           VQK++DELEQLL+DD+DMAELYLSRK+AG  +P   SG  I W+  SP   +K+S  K+ 
Sbjct: 198 VQKIKDELEQLLEDDEDMAELYLSRKLAGASSPAIDSGEHINWYPTSPTIGAKISRAKSH 257

Query: 277 RASAITFRGE--NDVEELEMLLEAYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQ 336
              + T RG+  NDVEE+EMLLEA+FMQI+ TLNKL  LREY+D+TED++NIQLD+ RNQ
Sbjct: 258 LVRSATVRGDDKNDVEEVEMLLEAHFMQIDRTLNKLTELREYVDETEDFLNIQLDSSRNQ 317

Query: 337 LIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLS 396
           LI+ E+ L++G++C+S+YS+V  I GMNIP+ W    +H+FKWVV  T  VCA ++V++ 
Sbjct: 318 LIKFEIILTAGSICVSVYSVVVGILGMNIPFPW-NIKKHMFKWVVSGTATVCAILFVTIM 377

Query: 397 SYARSKGLVG 400
           S+AR K L G
Sbjct: 378 SFARYKKLFG 379

BLAST of Carg16855 vs. ExPASy TrEMBL
Match: A0A6J1HGA4 (Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111463817 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.1e-215
Identity = 397/400 (99.25%), Postives = 398/400 (99.50%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           MAGDGFGFPVESQAVSVKKKT ATTTTTTKKKNTVVSRSWVSLDHEGR+TVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQAVSVKKKTAATTTTTTKKKNTVVSRSWVSLDHEGRSTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPS TQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSHTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 400

BLAST of Carg16855 vs. ExPASy TrEMBL
Match: A0A6J1HR33 (Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111467011 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 8.9e-213
Identity = 394/400 (98.50%), Postives = 394/400 (98.50%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           MAGDGFGFPVESQ VSVKKK   T TTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM
Sbjct: 1   MAGDGFGFPVESQVVSVKKK---TATTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETD YPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDAYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS
Sbjct: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 397

BLAST of Carg16855 vs. ExPASy TrEMBL
Match: A0A0A0LLQ5 (Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G225310 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.0e-202
Identity = 375/400 (93.75%), Postives = 381/400 (95.25%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           M GDGF  PVESQ  SVKKK      TTTKKK T VSRSWVSLD EGR+T+LDVDKY IM
Sbjct: 1   MGGDGFVIPVESQVSSVKKK---KKQTTTKKKTTAVSRSWVSLDREGRSTILDVDKYVIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           +RVQINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDPMDENVVPIV
Sbjct: 61  ERVQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSK+S+TSR SAIT RGENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKISRTSRVSAITIRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVI+TGIVCATIYVSLSSYAR KGLVGS
Sbjct: 361 TWKEDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLVGS 397

BLAST of Carg16855 vs. ExPASy TrEMBL
Match: A0A1S3BLU7 (Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491255 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 5.0e-200
Identity = 371/400 (92.75%), Postives = 380/400 (95.00%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           M GDGF  PVESQ  SVKKK      +TTKKK T VSRSWVSLD +GR+T+LDVDKYAIM
Sbjct: 1   MGGDGFVIPVESQVSSVKKK---KKQSTTKKKTTAVSRSWVSLDRDGRSTILDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           +R QINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDP DENVVPIV
Sbjct: 61  ERAQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPTDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELYLS 240

Query: 241 RKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIEG 300
           RKVAGTPESGSGTPIWFLASPKDYSK+S+TSR SAIT RGENDVEELEMLLEAYFMQIEG
Sbjct: 241 RKVAGTPESGSGTPIWFLASPKDYSKISRTSRVSAITIRGENDVEELEMLLEAYFMQIEG 300

Query: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360
           TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY
Sbjct: 301 TLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPY 360

Query: 361 TWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           TWKEDHEHVFKWVVI+TGIVCATIYVSLSSYAR KGLVG+
Sbjct: 361 TWKEDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLVGT 397

BLAST of Carg16855 vs. ExPASy TrEMBL
Match: A0A5D3E2W6 (Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001010 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.2e-198
Identity = 371/401 (92.52%), Postives = 380/401 (94.76%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           M GDGF  PVESQ  SVKKK      +TTKKK T VSRSWVSLD +GR+T+LDVDKYAIM
Sbjct: 1   MGGDGFVIPVESQVSSVKKK---KKQSTTKKKTTAVSRSWVSLDRDGRSTILDVDKYAIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
           +R QINARDLRLLDPLLSYPSTILGRE VIVLNLEHIKSIITADEVLLRDP DENVVPIV
Sbjct: 61  ERAQINARDLRLLDPLLSYPSTILGRERVIVLNLEHIKSIITADEVLLRDPTDENVVPIV 120

Query: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180
           EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL
Sbjct: 121 EELQRRLPSTNSLYQGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTREL 180

Query: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK-VRDELEQLLDDDDDMAELYL 240
           ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQK VRDELEQLLDDDDDMAELYL
Sbjct: 181 ETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVVRDELEQLLDDDDDMAELYL 240

Query: 241 SRKVAGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRGENDVEELEMLLEAYFMQIE 300
           SRKVAGTPESGSGTPIWFLASPKDYSK+S+TSR SAIT RGENDVEELEMLLEAYFMQIE
Sbjct: 241 SRKVAGTPESGSGTPIWFLASPKDYSKISRTSRVSAITIRGENDVEELEMLLEAYFMQIE 300

Query: 301 GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP 360
           GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP
Sbjct: 301 GTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIP 360

Query: 361 YTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           YTWKEDHEHVFKWVVI+TGIVCATIYVSLSSYAR KGLVG+
Sbjct: 361 YTWKEDHEHVFKWVVIVTGIVCATIYVSLSSYARYKGLVGT 398

BLAST of Carg16855 vs. TAIR 10
Match: AT5G64560.1 (magnesium transporter 9 )

HSP 1 Score: 495.0 bits (1273), Expect = 5.8e-140
Identity = 279/408 (68.38%), Postives = 318/408 (77.94%), Query Frame = 0

Query: 1   MAGDGFGFPVE-SQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAI 60
           MA +G+  P + S  V+VKKKT                 SW  +D  G++  LDVDKY I
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKT--------------PQASWALIDATGQSEPLDVDKYEI 60

Query: 61  MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120
           M RVQI+ARDLR+LDP LSYPSTILGRE  IVLNLEHIK+IIT++EVLLRDP DENV+P+
Sbjct: 61  MHRVQIHARDLRILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPV 120

Query: 121 VEELQRRLPSTNSLYQ-GQGEEEEPSTTQNE---LAENEFPFEFRALEVALEAICSFLDA 180
           VEEL+RRLP  N+ +  GQG+ +E +  QN+     E+E PFEFRALEVALEAICSFL A
Sbjct: 121 VEELRRRLPVGNASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAA 180

Query: 181 RTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMA 240
           RT ELET  YPALDELTSKISSRNLDRVRKLKSAMTRLT RVQKVRDELEQLLDDDDDMA
Sbjct: 181 RTAELETAAYPALDELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMA 240

Query: 241 ELYLSRKV--AGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRG-ENDVEELEMLLE 300
           +LYLSRK+  A +P S  G P W+  SP   SK+S+ SRAS  T  G ENDVEELEMLLE
Sbjct: 241 DLYLSRKLSSASSPISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLE 300

Query: 301 AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTA 360
           AYFMQI+ TLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  
Sbjct: 301 AYFMQIDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAG 360

Query: 361 IFGMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           IFGMNIPYTW + H ++FK+VV LTG +C  ++V + SYAR KGLVGS
Sbjct: 361 IFGMNIPYTWNDGHGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of Carg16855 vs. TAIR 10
Match: AT5G09690.1 (magnesium transporter 7 )

HSP 1 Score: 475.7 bits (1223), Expect = 3.6e-134
Identity = 264/406 (65.02%), Postives = 305/406 (75.12%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           M+ DG   PV+S AV            T K+K + +SRSW+S+D  G+ TVLDVDK+ IM
Sbjct: 1   MSPDGELVPVDSSAV-----------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
            RVQI+ARDLR+LDP L YPS ILGRE  IVLNLEHIK+IITA+EVL+RD  DENV+P++
Sbjct: 61  HRVQIHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVL 120

Query: 121 EELQRRLPSTNSLY--QGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTR 180
           EE QRRLP  N  +   G G+  E         E+E PFEFRALEVALEAICSFL ART 
Sbjct: 121 EEFQRRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTT 180

Query: 181 ELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELY 240
           ELE   YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LY
Sbjct: 181 ELEKFAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLY 240

Query: 241 LSRKVAGTPE--SGSGTPIWFLASPKDYSKMSKTSRASAITFRG--ENDVEELEMLLEAY 300
           L+RK+ G     S S  PIW+  SP   S +S+ SR S +T RG  E DVEELEMLLEAY
Sbjct: 241 LTRKLVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAY 300

Query: 301 FMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIF 360
           FMQI+ TLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IF
Sbjct: 301 FMQIDSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIF 360

Query: 361 GMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           GMNIP TW  DH ++FKWVV LTG  C  ++V + SYAR +GL+GS
Sbjct: 361 GMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of Carg16855 vs. TAIR 10
Match: AT5G09690.2 (magnesium transporter 7 )

HSP 1 Score: 475.7 bits (1223), Expect = 3.6e-134
Identity = 264/406 (65.02%), Postives = 305/406 (75.12%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           M+ DG   PV+S AV            T K+K + +SRSW+S+D  G+ TVLDVDK+ IM
Sbjct: 12  MSPDGELVPVDSSAV-----------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIM 71

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
            RVQI+ARDLR+LDP L YPS ILGRE  IVLNLEHIK+IITA+EVL+RD  DENV+P++
Sbjct: 72  HRVQIHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVL 131

Query: 121 EELQRRLPSTNSLY--QGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTR 180
           EE QRRLP  N  +   G G+  E         E+E PFEFRALEVALEAICSFL ART 
Sbjct: 132 EEFQRRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTT 191

Query: 181 ELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELY 240
           ELE   YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LY
Sbjct: 192 ELEKFAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLY 251

Query: 241 LSRKVAGTPE--SGSGTPIWFLASPKDYSKMSKTSRASAITFRG--ENDVEELEMLLEAY 300
           L+RK+ G     S S  PIW+  SP   S +S+ SR S +T RG  E DVEELEMLLEAY
Sbjct: 252 LTRKLVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAY 311

Query: 301 FMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIF 360
           FMQI+ TLNKL  LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IF
Sbjct: 312 FMQIDSTLNKLTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIF 371

Query: 361 GMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           GMNIP TW  DH ++FKWVV LTG  C  ++V + SYAR +GL+GS
Sbjct: 372 GMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 397

BLAST of Carg16855 vs. TAIR 10
Match: AT5G64560.2 (magnesium transporter 9 )

HSP 1 Score: 464.9 bits (1195), Expect = 6.4e-131
Identity = 263/381 (69.03%), Postives = 298/381 (78.22%), Query Frame = 0

Query: 1   MAGDGFGFPVE-SQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAI 60
           MA +G+  P + S  V+VKKKT                 SW  +D  G++  LDVDKY I
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKT--------------PQASWALIDATGQSEPLDVDKYEI 60

Query: 61  MQRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPI 120
           M RVQI+ARDLR+LDP LSYPSTILGRE  IVLNLEHIK+IIT++EVLLRDP DENV+P+
Sbjct: 61  MHRVQIHARDLRILDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPV 120

Query: 121 VEELQRRLPSTNSLYQ-GQGEEEEPSTTQNE---LAENEFPFEFRALEVALEAICSFLDA 180
           VEEL+RRLP  N+ +  GQG+ +E +  QN+     E+E PFEFRALEVALEAICSFL A
Sbjct: 121 VEELRRRLPVGNASHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAA 180

Query: 181 RTRELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMA 240
           RT ELET  YPALDELTSKISSRNLDRVRKLKSAMTRLT RVQKVRDELEQLLDDDDDMA
Sbjct: 181 RTAELETAAYPALDELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMA 240

Query: 241 ELYLSRKV--AGTPESGSGTPIWFLASPKDYSKMSKTSRASAITFRG-ENDVEELEMLLE 300
           +LYLSRK+  A +P S  G P W+  SP   SK+S+ SRAS  T  G ENDVEELEMLLE
Sbjct: 241 DLYLSRKLSSASSPISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLE 300

Query: 301 AYFMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTA 360
           AYFMQI+ TLN+L TLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  
Sbjct: 301 AYFMQIDSTLNRLTTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAG 360

Query: 361 IFGMNIPYTWKEDHEHVFKWV 374
           IFGMNIPYTW + H ++FK+V
Sbjct: 361 IFGMNIPYTWNDGHGYMFKYV 367

BLAST of Carg16855 vs. TAIR 10
Match: AT5G09690.4 (magnesium transporter 7 )

HSP 1 Score: 444.5 bits (1142), Expect = 9.0e-125
Identity = 253/406 (62.32%), Postives = 293/406 (72.17%), Query Frame = 0

Query: 1   MAGDGFGFPVESQAVSVKKKTVATTTTTTKKKNTVVSRSWVSLDHEGRTTVLDVDKYAIM 60
           M+ DG   PV+S AV            T K+K + +SRSW+S+D  G+ TVLDVDK+ IM
Sbjct: 1   MSPDGELVPVDSSAV-----------VTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIM 60

Query: 61  QRVQINARDLRLLDPLLSYPSTILGRESVIVLNLEHIKSIITADEVLLRDPMDENVVPIV 120
            RVQI+ARDLR+LDP L YPS ILGRE  IVLNLEHIK+IITA+EVL+RD  DENV+P++
Sbjct: 61  HRVQIHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVL 120

Query: 121 EELQRRLPSTNSLY--QGQGEEEEPSTTQNELAENEFPFEFRALEVALEAICSFLDARTR 180
           EE QRRLP  N  +   G G+  E         E+E PFEFRALEVALEAICSFL ART 
Sbjct: 121 EEFQRRLPVGNEAHGVHGDGDLGE---------EDESPFEFRALEVALEAICSFLAARTT 180

Query: 181 ELETDTYPALDELTSKISSRNLDRVRKLKSAMTRLTNRVQKVRDELEQLLDDDDDMAELY 240
           ELE   YPALDELT KISSRNL+RVRKLKSAMTRLT RVQKVRDELEQLLDDD DMA+LY
Sbjct: 181 ELEKFAYPALDELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLY 240

Query: 241 LSRKVAGTPE--SGSGTPIWFLASPKDYSKMSKTSRASAITFRG--ENDVEELEMLLEAY 300
           L+RK+ G     S S  PIW+  SP   S +S+ SR S +T RG  E DVEELEMLLE  
Sbjct: 241 LTRKLVGASSSVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLE-- 300

Query: 301 FMQIEGTLNKLITLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIF 360
                        LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+S+YS++  IF
Sbjct: 301 -------------LREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIF 360

Query: 361 GMNIPYTWKEDHEHVFKWVVILTGIVCATIYVSLSSYARSKGLVGS 401
           GMNIP TW  DH ++FKWVV LTG  C  ++V + SYAR +GL+GS
Sbjct: 361 GMNIPNTWNHDHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6571485.14.2e-217100.00Magnesium transporter MRS2-7, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022963506.12.3e-21599.25magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata][more]
XP_023554091.11.5e-21499.00magnesium transporter MRS2-I-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022967532.11.8e-21298.50magnesium transporter MRS2-I-like isoform X1 [Cucurbita maxima][more]
XP_038888713.11.5e-20194.50magnesium transporter MRS2-I-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q10D384.2e-14373.73Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I ... [more]
B8AJT91.6e-14271.61Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE... [more]
Q9FLG28.2e-13968.38Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1[more]
Q304A05.1e-13365.02Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1[more]
P0CZ213.9e-10960.00Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HGA41.1e-21599.25Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111463817 PE=3 SV=1[more]
A0A6J1HR338.9e-21398.50Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111467011 PE=3 SV=1[more]
A0A0A0LLQ57.0e-20293.75Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G225310 PE=3 SV=1[more]
A0A1S3BLU75.0e-20092.75Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491255 PE=3 SV=1[more]
A0A5D3E2W61.2e-19892.52Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21... [more]
Match NameE-valueIdentityDescription
AT5G64560.15.8e-14068.38magnesium transporter 9 [more]
AT5G09690.13.6e-13465.02magnesium transporter 7 [more]
AT5G09690.23.6e-13465.02magnesium transporter 7 [more]
AT5G64560.26.4e-13169.03magnesium transporter 9 [more]
AT5G09690.49.0e-12562.32magnesium transporter 7 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 203..230
NoneNo IPR availableGENE3D2.40.128.330coord: 22..111
e-value: 6.3E-20
score: 72.4
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 337..391
e-value: 4.2E-5
score: 25.6
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 114..330
e-value: 9.4E-68
score: 229.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..148
NoneNo IPR availablePANTHERPTHR13890:SF46MAGNESIUM TRANSPORTER MRS2-LIKE PROTEINcoord: 25..400
NoneNo IPR availableSUPERFAMILY144083Magnesium transport protein CorA, transmembrane regioncoord: 336..387
IPR002523Mg2+ transporter protein, CorA-like/Zinc transport protein ZntBPFAMPF01544CorAcoord: 287..385
e-value: 7.7E-7
score: 28.8
IPR039204Magnesium transporter MRS2-likePANTHERPTHR13890RNA SPLICING PROTEIN MRS2, MITOCHONDRIALcoord: 25..400
IPR039204Magnesium transporter MRS2-likeCDDcd12823Mrs2_Mfm1p-likecoord: 40..397
e-value: 1.84259E-102
score: 304.552

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16855-RACarg16855-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity