Carg16768 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16768
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Fe2OG dioxygenase domain-containing protein
Location	Carg_Chr16: 1128021 .. 1129599 (-)
RNA-Seq Expression	Carg16768
Synteny	Carg16768

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGCTAAGAGGTGAGAGTTCAGGGTCATTTCCCACAGGACAGACTGCACAAGAGAAAGGCTTAAACTATGTCCCTAATGCTTATGTGATTCCTACCCCACAGCGCCCGAGTTTGTCCCCGGAGACCGCCATTGTTCCCATCATTGACATGGCTTCTCTAAGATCAAATGATTCAGCTCAAAGGTCCCTCACCATTGAAGCGCTCAGAAAAGCTTGCATTAGCCTCGGCTTCTTTCAGGTAAAGTTTCAAGCAACCTACCTCTTTTATATTAGCTAGCTCTTAACTTCTATTTGTTTTTCTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTAAACCAAGCCAGGGAGTTCTTTAACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTAGCAAAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTGGACAAAATAAAGTTTTGGAGAGTTTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGGTAGACTCCTGGCCCACCAATCCACGTACTTATAGGTAACTCAATATTTTAATTCTCCATCTCCACTTCTGACCCAATTTCCATTCTTTTTCTTTTCCTGATTACCATGCAAAATTTACTTACATGTGAAGGCTGTACAATGAAATTCAACAGAAAGATACAATAAAACCATTGGACCGCCAAAGAGATCACCGCTATGCATATATGCTGACGCCTATGAACCAGAACTTCAGCTAACAACGCTATTTTTAATTATGCTAACATTGAGTATGGTCTAAAAGTTTGGACTCTTTTTATGCATACTCACCGTACTTGAGCATTCTTCTACAATGAACCATAAATATAGGACTTGAAATGCACTCACCAATAAATGACAATCAAAACAGGACAGAAAGCACTACTTACCGACAAAAATATGGACATCTCTGATTAAAATATCAATATAAAAATCATCCCCCCCAAAAAAAAAACTATTATTTAATCTCTTACTTTGAGAAAACCACATATGACTTGAAATTTTATAAGATCCTAGGTGCATACGTTCTTCAGAGACATGTCCTTGTGTTTTACATGTATATATATACATATTATATATATATATATGTGTATGACCGTTTCAGGGAAAAGATGGGACAATACACCAAGGAGGTGAGAAAAGTAAGCTTAGAGATAGAGGAAGCCATAACTGAAAGCTTGGGTTTAAGACCAACATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGGCAGTAAACTGCTACCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTATAGCTGCATAACCACAATTCTGCAAAGCAGCCAGGGACTTGAAATCATGGACCGGGCTGATGGGAAATGGAAGATGGTTCCTAATGTTGATGGCAGTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTAACTAAATTAAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAACGATCTTGCAGAAGGAAAGAGCTTCATCGACACACTCAAGATCCAGGATTAA

mRNA sequence

ATGGGGCTAAGAGGTGAGAGTTCAGGGTCATTTCCCACAGGACAGACTGCACAAGAGAAAGGCTTAAACTATGTCCCTAATGCTTATGTGATTCCTACCCCACAGCGCCCGAGTTTGTCCCCGGAGACCGCCATTGTTCCCATCATTGACATGGCTTCTCTAAGATCAAATGATTCAGCTCAAAGGTCCCTCACCATTGAAGCGCTCAGAAAAGCTTGCATTAGCCTCGGCTTCTTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTAAACCAAGCCAGGGAGTTCTTTAACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTAGCAAAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTGGACAAAATAAAGTTTTGGAGAGTTTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGGTAGACTCCTGGCCCACCAATCCACGTACTTATAGACCAACATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGGCAGTAAACTGCTACCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTATAGCTGCATAACCACAATTCTGCAAAGCAGCCAGGGACTTGAAATCATGGACCGGGCTGATGGGAAATGGAAGATGGTTCCTAATGTTGATGGCAGTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTAACTAAATTAAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAACGATCTTGCAGAAGGAAAGAGCTTCATCGACACACTCAAGATCCAGGATTAA

Coding sequence (CDS)

ATGGGGCTAAGAGGTGAGAGTTCAGGGTCATTTCCCACAGGACAGACTGCACAAGAGAAAGGCTTAAACTATGTCCCTAATGCTTATGTGATTCCTACCCCACAGCGCCCGAGTTTGTCCCCGGAGACCGCCATTGTTCCCATCATTGACATGGCTTCTCTAAGATCAAATGATTCAGCTCAAAGGTCCCTCACCATTGAAGCGCTCAGAAAAGCTTGCATTAGCCTCGGCTTCTTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTAAACCAAGCCAGGGAGTTCTTTAACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTAGCAAAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTGGACAAAATAAAGTTTTGGAGAGTTTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGGTAGACTCCTGGCCCACCAATCCACGTACTTATAGACCAACATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGGCAGTAAACTGCTACCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTATAGCTGCATAACCACAATTCTGCAAAGCAGCCAGGGACTTGAAATCATGGACCGGGCTGATGGGAAATGGAAGATGGTTCCTAATGTTGATGGCAGTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTAACTAAATTAAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAACGATCTTGCAGAAGGAAAGAGCTTCATCGACACACTCAAGATCCAGGATTAA

Protein sequence

MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSAQRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYRPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVTKLKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQD

Homology

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BLAST of Carg16768 vs. NCBI nr
Match: KAG7014854.1 (Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 571.6 bits (1472), Expect = 3.7e-159
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKA 120
           QRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKA
Sbjct: 61  QRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYRPTYLSSKMAEGVQVVAV 180
           VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYRPTYLSSKMAEGVQVVAV
Sbjct: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYRPTYLSSKMAEGVQVVAV 180

Query: 181 NCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGD 240
           NCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGD
Sbjct: 181 NCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGD 240

Query: 241 HVEVTKLKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQD 281
           HVEVTKLKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQD
Sbjct: 241 HVEVTKLKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQD 280

[Close]

BLAST of Carg16768 vs. NCBI nr
Match: XP_022922584.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata])

HSP 1 Score: 510.8 bits (1314), Expect = 7.7e-141
Identity = 274/376 (72.87%), Postives = 276/376 (73.40%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL+GESSGSF TGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLTIEALRKACISLGFF----------------------QIINHGISKTVMEEALNQA 120
           QRSLTIEALRKACISLGFF                      QIINHGISKTVMEEALNQA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180
           REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PRTY----------------------------RPTYLSSKMAEGVQVVAVNCYPPCPQPG 240
           P TY                            RPTYLSSKMAEGVQVVAVNCYPPCPQPG
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV------ 281
           VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV      
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

[Close]

BLAST of Carg16768 vs. NCBI nr
Match: XP_023552054.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.4 bits (1287), Expect = 1.0e-137
Identity = 269/379 (70.98%), Postives = 273/379 (72.03%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRS+DSA
Sbjct: 62  MGLGGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSDDSA 121

Query: 61  QRSLTIEALRKACISLGFF-------------------------QIINHGISKTVMEEAL 120
           QRSL IE LRKACIS+GFF                         QIINHGISKTVMEEAL
Sbjct: 122 QRSLAIEVLRKACISIGFFQVKFQATYLPTSFILASSXLQFVFLQIINHGISKTVMEEAL 181

Query: 121 NQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW 180
           +QAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW
Sbjct: 182 SQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW 241

Query: 181 PTNPRTY----------------------------RPTYLSSKMAEGVQVVAVNCYPPCP 240
           PTNP TY                            RPTYLSSKMAEGVQVVAVNCYPPCP
Sbjct: 242 PTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCP 301

Query: 241 QPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV--- 281
           QPGVALGLPPHTDYSCITTILQSSQGLEIMD ADGKWKMVPNVDGSLQVHVGDHVEV   
Sbjct: 302 QPGVALGLPPHTDYSCITTILQSSQGLEIMDPADGKWKMVPNVDGSLQVHVGDHVEVLSN 361

[Close]

BLAST of Carg16768 vs. NCBI nr
Match: XP_022984408.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 6.7e-137
Identity = 269/381 (70.60%), Postives = 273/381 (71.65%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGLRGESSGSF TGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLTIEALRKACISLGFF----------------------------QIINHGISKTVME 120
           +RSL IEALRKACISLGFF                            QIINHGISKTVME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180
           EAL+QAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 DSWPTNPRTYRP---------------------------TYLSSKMAEGVQVVAVNCYPP 240
           DSWPTNP TYR                             YLSSKMAEGVQVVAVNCYPP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV- 281
           CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV 
Sbjct: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL 300

[Close]

BLAST of Carg16768 vs. NCBI nr
Match: XP_004146935.2 (flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa_014422 [Cucumis sativus])

HSP 1 Score: 480.7 bits (1236), Expect = 8.5e-132
Identity = 248/354 (70.06%), Postives = 265/354 (74.86%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GESSG+FPTG+TAQEKGL+YVPNAYVIP P RPS+SPE AIVPIIDMASLRS+DS 
Sbjct: 3   MGLNGESSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSMSPEIAIVPIIDMASLRSSDSV 62

Query: 61  QRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKA 120
           QRSL  E LRKACISLGFFQIINHGI++TVMEEAL+QA EFFNLPLKEKMKYKSDDV KA
Sbjct: 63  QRSLATEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLKEKMKYKSDDVCKA 122

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYR----------------- 180
           VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+DSWPTNPR YR                 
Sbjct: 123 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGRYTKEVRKVSLEI 182

Query: 181 -----------PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ 240
                      PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT+LQSSQ
Sbjct: 183 MEAINESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 242

Query: 241 GLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV------------------------TKL- 281
           GLE+MD ADG WKMVP +DG+LQVHVGDHVEV                        T L 
Sbjct: 243 GLEVMDPADGTWKMVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 302

[Close]

BLAST of Carg16768 vs. ExPASy Swiss-Prot
Match: Q7XR84 (Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.9e-50
Identity = 114/322 (35.40%), Postives = 161/322 (50.00%), Query Frame = 0


Query: 31  IPTPQRPSLSPETAIVPIIDMASLRSNDSAQRSLTIEALRKACISLGFFQIINHGISKTV 90
           IP  Q PS       +P+I++  L  +D   RS  +  + KAC  LG+FQ+I+HGIS++V
Sbjct: 8   IPQEQLPSQELHPPPMPVINLGHLSLDDPTVRSRVVNDIAKACRDLGYFQVISHGISQSV 67

Query: 91  MEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLED 150
           M+ A+  A EFF LP + K +Y SDD+ + VRY TS KDG   I   R FLKHYAHPL D
Sbjct: 68  MDGAIEAASEFFKLPNEIKKEYASDDIRQPVRYDTSSKDG---ISMSRAFLKHYAHPLCD 127

Query: 151 WVDSWPTNPRTYR----------------------------PTYLSSKMAEGVQVVAVNC 210
           W+  WP  P  YR                              Y+  K  EG+Q+++VNC
Sbjct: 128 WLQYWPQQPPIYREYMAKYAVEVRVVALKLMEAILEGLGIGKEYMQEKFEEGLQLLSVNC 187

Query: 211 YPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHV 270
           YP   Q   ++GL  H+DY  +T +L S QGLE++DR+   WK+V  +  +L VHVGDH+
Sbjct: 188 YPKVSQSDTSIGLAAHSDYGLLTILLTSCQGLEVVDRSSNSWKVVQQLPHALHVHVGDHM 247

Query: 271 EV----------------------------------------------TKLKYKESSFND 279
           EV                                                 KYKE+SFND
Sbjct: 248 EVLSNGRIKTVVHRAVLNPQEARISLASIHGFALHEKVSSAKELVDEENPQKYKENSFND 307

[Close]

BLAST of Carg16768 vs. ExPASy Swiss-Prot
Match: Q7XR83 (2-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD21 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.3e-45
Identity = 113/317 (35.65%), Postives = 157/317 (49.53%), Query Frame = 0


Query: 37  PSLSPETAIVPIIDMASLRSNDSAQRSLTIEALRKACISLGFFQIINHGISKTVMEEALN 96
           P L P    +P+I++  L + DSA R+L +  +  AC   G F+++NHGIS++ M  AL 
Sbjct: 19  PPLPPPPRPLPVINLGRL-TMDSASRALAVRDIVLACRERGCFEVVNHGISRSCMNGALE 78

Query: 97  QAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWP 156
            A EFF L  + K ++ SDD+ + +RY TS +DG   I   R FLKHYA+PL+DW+  WP
Sbjct: 79  AASEFFQLSTERKEEFASDDIRQPIRYDTSSRDG---ISMSRSFLKHYANPLDDWIKFWP 138

Query: 157 TNPRTYR----------------------------PTYLSSKMAEGVQVVAVNCYP-PCP 216
           T P TYR                            P+YL  K+  GVQ VA+N YP    
Sbjct: 139 TQPPTYREKMGEYAVETQRVSMQLMEAILQGLGLGPSYLQEKLEGGVQFVALNNYPQSSA 198

Query: 217 QPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVT-- 276
           +    +GL PH+DY  +T +LQSS GLE+M   D  W  VP + G+L VHVGDH+EV   
Sbjct: 199 KKADKIGLAPHSDYGFLTILLQSSPGLEVMHHEDDAWTSVPAIPGALHVHVGDHLEVLSN 258

Query: 277 -KLK-------------------------------------------YKESSFNDFLKFL 279
            +LK                                           Y+ SSF DFL FL
Sbjct: 259 GQLKSLVHRAVLNPNESRISIASIHGLSMDEEVHCAEELVDEHHPKMYRGSSFQDFLDFL 318

[Close]

BLAST of Carg16768 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 7.9e-40
Identity = 93/262 (35.50%), Postives = 132/262 (50.38%), Query Frame = 0


Query: 19  EKGLNYVPNAYVIPTPQRPSLSPETAI-------VPIIDMASLRSNDSAQRSLTIEALRK 78
           + G+  +P  YV+P   RP  +   A        +P++D++ LR    ++R   +  L  
Sbjct: 46  DSGITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRV--PSERGAVLRTLDA 105

Query: 79  ACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGV 138
           AC   GFFQ++NHG+   V+   L+ AR FF LP  E+ +Y S DV   VRYGTS     
Sbjct: 106 ACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVR 165

Query: 139 DKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYRPTY------------------------- 198
           D +  WR FLK    PL   V+SWPT+P   R                            
Sbjct: 166 DAVLCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVG 225

Query: 199 ---LSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSS-QGLEIMDRADG 245
              +   +A G Q++ VNCYP CPQP + LG+PPH+DY  +T +LQ    GL++M    G
Sbjct: 226 GGGVMEDLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHA--G 285

[Close]

BLAST of Carg16768 vs. ExPASy Swiss-Prot
Match: Q651B6 (Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC2 PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.4e-36
Identity = 98/279 (35.13%), Postives = 143/279 (51.25%), Query Frame = 0


Query: 5   GESSGS--FPTG-QTAQEKGLNYVPNAYVIPTPQRPS--LSPETAIVPIIDMASLRSNDS 64
           GE SGS  +  G +   + G+  +P  YV+P   RP+  ++P  A++P++D+A+LR+ D 
Sbjct: 13  GEGSGSSEWELGVRQLCDSGITTLPARYVLPPADRPARYVTP-PALLPVVDLAALRARDP 72

Query: 65  AQRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSK 124
            Q    + AL  AC   GFFQ++NHG+    M  A    R FF+LPL  + +Y S D+  
Sbjct: 73  CQ----LAALHAACRDYGFFQLLNHGVPPDAMLYAAR--RFFFDLPLPARKRYMSADIRA 132

Query: 125 AVRYGTSLKDGVDKIKFWRVFLK---HYAHPLEDWVDSWPTNPRTYRPT----------- 184
           AVRYGTS     D +  WR FLK        L D + SWP  P   RP            
Sbjct: 133 AVRYGTSFNQLNDAVLSWRDFLKLLIRDTRRLADVLPSWPDAPDDLRPAAAAYATACQRL 192

Query: 185 -------------------YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT 244
                               L  +   G Q++ VNC+P CP+P + LG+PPH+DY  +T 
Sbjct: 193 FRELMEAALDALGIVRCRRQLLEECDAGSQMMMVNCFPACPEPELTLGMPPHSDYGLLTI 252

[Close]

BLAST of Carg16768 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.1e-36
Identity = 82/251 (32.67%), Postives = 136/251 (54.18%), Query Frame = 0


Query: 25  VPNAYVIPTPQRPSLSPETAI--VPIIDMASLRSNDSAQRSLTIEALRKACISLGFFQII 84
           +P  YV P   RP LS  + +   P+ID++      S  RS  I+ + +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E ++ AREFF++ ++EKMK  SDD +K  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWVDSWPTNPRTYR----------------------------PTYLSSKMAEG 204
            + +P+  +V+ WP+NP +++                              Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ--GLEIMDRADGKWKMVPNVDG 244
            Q +AVN YPPCP+P +  GLP HTD + +T +LQ +   GL+I+   DG+W  V     
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQIL--IDGQWFAVNPHPD 254

[Close]

BLAST of Carg16768 vs. ExPASy TrEMBL
Match: A0A6J1E3T8 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430549 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 3.7e-141
Identity = 274/376 (72.87%), Postives = 276/376 (73.40%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL+GESSGSF TGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLTIEALRKACISLGFF----------------------QIINHGISKTVMEEALNQA 120
           QRSLTIEALRKACISLGFF                      QIINHGISKTVMEEALNQA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180
           REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PRTY----------------------------RPTYLSSKMAEGVQVVAVNCYPPCPQPG 240
           P TY                            RPTYLSSKMAEGVQVVAVNCYPPCPQPG
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV------ 281
           VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV      
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

[Close]

BLAST of Carg16768 vs. ExPASy TrEMBL
Match: A0A6J1JAE6 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LOC111482715 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.3e-137
Identity = 269/381 (70.60%), Postives = 273/381 (71.65%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGLRGESSGSF TGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLTIEALRKACISLGFF----------------------------QIINHGISKTVME 120
           +RSL IEALRKACISLGFF                            QIINHGISKTVME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180
           EAL+QAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 DSWPTNPRTYRP---------------------------TYLSSKMAEGVQVVAVNCYPP 240
           DSWPTNP TYR                             YLSSKMAEGVQVVAVNCYPP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV- 281
           CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV 
Sbjct: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL 300

[Close]

BLAST of Carg16768 vs. ExPASy TrEMBL
Match: A0A5A7TMH3 (Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00340 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.9e-131
Identity = 248/354 (70.06%), Postives = 262/354 (74.01%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GE SG+FPTG+TAQEKGL+YVPNAYVIP P RPSLSPE AIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKA 120
           QRSL +E LRKACISLGFFQIINHGI++TVMEEAL+QA EFFNLPL EKMKYKSDDV KA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYR----------------- 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDW+DSWPTNPR YR                 
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 -----------PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ 240
                      PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT+LQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV------------------------TKL- 281
           GLEIMD ADG WKMV  +DG+LQVHVGDHVEV                        T L 
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

[Close]

BLAST of Carg16768 vs. ExPASy TrEMBL
Match: A0A1S3BX86 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.9e-131
Identity = 248/354 (70.06%), Postives = 262/354 (74.01%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GE SG+FPTG+TAQEKGL+YVPNAYVIP P RPSLSPE AIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKA 120
           QRSL +E LRKACISLGFFQIINHGI++TVMEEAL+QA EFFNLPL EKMKYKSDDV KA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYR----------------- 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDW+DSWPTNPR YR                 
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 -----------PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ 240
                      PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT+LQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV------------------------TKL- 281
           GLEIMD ADG WKMV  +DG+LQVHVGDHVEV                        T L 
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

[Close]

BLAST of Carg16768 vs. ExPASy TrEMBL
Match: A0A6J1CIV6 (flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 7.3e-121
Identity = 241/381 (63.25%), Postives = 260/381 (68.24%), Query Frame = 0


Query: 1   MGLRGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GESSGSFPTG++AQEKGL+YVPNAYVIP P R S S ETAI+PIIDMASLRS DSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRSLTIEALRKACISLGFF---------------------------QIINHGISKTVMEE 120
           QRSL IE LR+ACI LGFF                           QIINH I++ VMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVD 180
           AL QA EFF+LPLKEKMKYKSDDVS+AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+ 
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 SWPTNPRTYR----------------------------PTYLSSKMAEGVQVVAVNCYPP 240
           SWPT+PR+YR                            PTYLSSKMAEGVQVVAVNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV- 281
           CPQP VALGLPPHTDYSCITTILQSS+GLEIMD ADG WK+VPN+DG+LQVH+GDHVEV 
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

[Close]

BLAST of Carg16768 vs. TAIR 10
Match: AT5G07480.1 (KAR-UP oxidoreductase 1 )

HSP 1 Score: 264.2 bits (674), Expect = 1.2e-70
Identity = 147/356 (41.29%), Postives = 190/356 (53.37%), Query Frame = 0


Query: 4   RGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSAQRS 63
           +GE S +F  G +AQE+ L YVP+ YV+P   +P  S  + IVP ID++ L+  D  +R 
Sbjct: 3   KGEDSSTFKMGNSAQERSLPYVPDCYVVPPSSKPCDS-NSGIVPTIDVSRLKGGDDERRG 62

Query: 64  LTIEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRY 123
                            I+NHGI++ ++++AL  A  FF LP KEK ++ S+DV   VRY
Sbjct: 63  -----------------IVNHGINQNILDDALEVANSFFELPAKEKKQFMSNDVYAPVRY 122

Query: 124 GTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYRP------------------- 183
            TSLKDG+D I+FWR+FLKHYAHPL  W+  WP NP  YR                    
Sbjct: 123 STSLKDGLDTIQFWRIFLKHYAHPLHRWIHLWPENPPGYREKMGKFCEEVRKLSIELMGA 182

Query: 184 ---------TYLSSKMAE-GVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGL 243
                     YLSS+M E G+QV+ VNCYPPCP P  ALGLPPH+DYSCIT +LQ+  GL
Sbjct: 183 ITESLGLGRDYLSSRMDENGMQVMTVNCYPPCPDPETALGLPPHSDYSCITLLLQNLDGL 242

Query: 244 EIMD----RADGKWKMVPNVDGSLQVHVGDHVEV-------------------------- 281
           +I D       G+W  VP V G L+VH+GDHVEV                          
Sbjct: 243 KIFDPMAHGGSGRWVGVPQVTGVLKVHIGDHVEVLSNGLYKSIVHKVTLNEEKTRISLAS 302

[Close]

BLAST of Carg16768 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 2.9e-61
Identity = 133/346 (38.44%), Postives = 184/346 (53.18%), Query Frame = 0


Query: 10  SFPTGQTAQEKGLNYVPNAYVIPTPQRP----SLSPETAIVPIIDMASLRSNDSAQRSLT 69
           SF +  T    G+  VP+ YV+P  QRP    SL      +P+ID++ L  +    RSL 
Sbjct: 12  SFTSAMTLTNSGVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLL--HQPFLRSLA 71

Query: 70  IEALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGT 129
           I  +  AC   GFFQ+INHGI  +V+ +AL+ A +FF+LP++EKM   S +V + VRYGT
Sbjct: 72  IHEISMACKEFGFFQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGT 131

Query: 130 SLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYR---------------------- 189
           SL    D++ +WR F+KHY+HPL  W+D WP+NP  Y+                      
Sbjct: 132 SLNHSTDRVHYWRDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAIS 191

Query: 190 ------PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIM 249
                   YL  ++ EG QV+AVNCYP CP+P +ALG+PPH+D+S +T +LQSS+GL+IM
Sbjct: 192 ESLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIM 251

Query: 250 DRADGKWKMVPNVDGSLQVHVGDHVEV--------------------------------- 278
           D  +  W  VP ++G+L V +GD VEV                                 
Sbjct: 252 D-CNKNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLH 311

[Close]

BLAST of Carg16768 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 228.0 bits (580), Expect = 9.2e-60
Identity = 128/345 (37.10%), Postives = 179/345 (51.88%), Query Frame = 0


Query: 11  FPTGQTAQEKGLNYVPNAYVIPTPQRPSLSP----ETAIVPIIDMASLRSNDSAQRSLTI 70
           F +     E G  +VP  Y++P  QRP L P     T  +P+ID++ L  +D   R   I
Sbjct: 13  FTSAMELTESGDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFL--HDPLLRLCVI 72

Query: 71  EALRKACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTS 130
             +  AC   GFFQ+INHGIS  V+++A + A  FF+LP  EKM   SD+  + VRYGTS
Sbjct: 73  HEIELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVRYGTS 132

Query: 131 LKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYR----------------------- 190
           +    D++ +WR F+KHY+HPL +W++ WP+NP  Y+                       
Sbjct: 133 INHSTDRVHYWRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISE 192

Query: 191 -----PTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMD 250
                  YL  ++ EG QV+AVNCYP CP+P +ALG+PPH+DY  +T +LQSS+GL+I D
Sbjct: 193 SLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKD 252

Query: 251 RADGKWKMVPNVDGSLQVHVGDHVEV---------------------------------- 278
             +  W  VP ++G+L V +GD VEV                                  
Sbjct: 253 -CNNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHK 312

[Close]

BLAST of Carg16768 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.0e-46
Identity = 99/264 (37.50%), Postives = 141/264 (53.41%), Query Frame = 0


Query: 19  EKGLNYVPNAYVIPTPQRPSLSPETAIV--------PIIDMASLRSNDSAQRSLTIEALR 78
           E GL  VP  Y+ P P RP L+    ++        P+ID A L       R   +  + 
Sbjct: 26  ENGLTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAELL---GPNRPHVLRTIA 85

Query: 79  KACISLGFFQIINHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDG 138
           +AC + GFFQ++NHG+   V +  ++  + FF LP +E+ KY S D+S  VRYGTS    
Sbjct: 86  EACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYGTSFNQI 145

Query: 139 VDKIKFWRVFLKHYAHPLEDWVDSWPTNPRTYR---PTY--------------------- 198
            D +  WR FLK YAHPL D++  WP++P  +R    TY                     
Sbjct: 146 KDNVFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAILESLEI 205

Query: 199 -----LSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSS-QGLEIMDRA 245
                 + ++ EG QVV VNCYPPCP+P + LG+PPH+DY  +T +LQ   +GL+I+ R 
Sbjct: 206 DGSDEAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEGLQILYR- 265

[Close]

BLAST of Carg16768 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.9e-37
Identity = 82/251 (32.67%), Postives = 136/251 (54.18%), Query Frame = 0


Query: 25  VPNAYVIPTPQRPSLSPETAI--VPIIDMASLRSNDSAQRSLTIEALRKACISLGFFQII 84
           +P  YV P   RP LS  + +   P+ID++      S  RS  I+ + +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALNQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E ++ AREFF++ ++EKMK  SDD +K  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWVDSWPTNPRTYR----------------------------PTYLSSKMAEG 204
            + +P+  +V+ WP+NP +++                              Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ--GLEIMDRADGKWKMVPNVDG 244
            Q +AVN YPPCP+P +  GLP HTD + +T +LQ +   GL+I+   DG+W  V     
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQIL--IDGQWFAVNPHPD 254

The following BLAST results are available for this feature:

BLAST of Carg16768 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014854.1	3.7e-159	100.00	Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022922584.1	7.7e-141	72.87	LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata]	[more]
XP_023552054.1	1.0e-137	70.98	LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022984408.1	6.7e-137	70.60	LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita maxima]	[more]
XP_004146935.2	8.5e-132	70.06	flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa...	[more]

BLAST of Carg16768 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XR84	2.9e-50	35.40	Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1...	[more]
Q7XR83	6.3e-45	35.65	2-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. ja...	[more]
Q6YYX9	7.9e-40	35.50	Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni...	[more]
Q651B6	1.4e-36	35.13	Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japoni...	[more]
Q9FLV0	4.1e-36	32.67	Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S...	[more]

BLAST of Carg16768 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E3T8	3.7e-141	72.87	LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JAE6	3.3e-137	70.60	LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5A7TMH3	5.9e-131	70.06	Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BX86	5.9e-131	70.06	protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 S...	[more]
A0A6J1CIV6	7.3e-121	63.25	flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV...	[more]

BLAST of Carg16768 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07480.1	1.2e-70	41.29	KAR-UP oxidoreductase 1	[more]
AT2G44800.1	2.9e-61	38.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G60290.1	9.2e-60	37.10	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G36690.1	2.0e-46	37.50	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G24530.1	2.9e-37	32.67	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 167..265 e-value: 6.0E-18 score: 67.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 14..166 e-value: 5.6E-33 score: 116.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 178..248 e-value: 1.7E-13 score: 50.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 46..156 e-value: 3.0E-25 score: 89.3
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 10..163 coord: 164..244
None	No IPR available	PANTHER	PTHR47991:SF67	KAR-UP OXIDOREDUCTASE 1	coord: 10..163 coord: 164..244
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 16..244

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16768-RA	Carg16768-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity