Carg16767 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16767
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	pre-mRNA-splicing factor CWC25 homolog
Location	Carg_Chr16: 1124538 .. 1126542 (-)
RNA-Seq Expression	Carg16767
Synteny	Carg16767

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACCGATCGACGAGCTGGAACCGATTCTCCAATGAATACTACTCGCATTCGGCCCCGTCCTCGAAGTCAGGGCAGGCGTCGCGATTGTGCTTGTCCTTTGACGGCAATGAGCTGCCAATGAATGATCCGGTCGCCGAGATGATGAAGCGAGAGAAAGCGCGAGTCAAGTTCGCCGAATCTGCCGTTCATGTGATTCCGCTCGTTTTGCTTCTCTGCACCTTGTTTTCTGGTTCATCTCAAATCCAGGTAAGTGAAGATCATGACGCAGGTAGAATTCGAATTCTTCTCCTTGGAATCTTTCCTTCCCAGCATTGATTTTCCGGGAAAATGAAGAGAAATTTCCCCGTTTCCACTAATCCAACGAATTAATCCATTCGCTTTCCGAGAAGAAAAAACAAATTGTTTGAGGATAAGCCTCGATTCCAAATGAAGAATGGAGTACATTTGATCCCTGCTTAGCGGTGAGCAACTGAACTTGTAAGAACAATCCTATTCCAGGGCTCAATGAATCTGTGATTTACTTGATGAATGCCTTTTTATTTCCTCCTTACAAATTTAACACTATCATAGTTCGTAGAACTTATAAAATTGTGAACACGGGGCGTATATCTTAATTGAAATACAGGCTTCCATTCAAAGATTTGAAAATTGAGAAAGTTTCTGAAACTACGTCCATCGGAATTGCTCACGATTATTATTGAGATCATTAAATTTAATTTTTTGAATTTTAGCGGCGAGAATACCTAGAGAATGCGATATTATTTTTTTATTAAAATTTAAGGTGAAAACTATTAATTGTATTTTATGTTATTCAGTGTTCATCAATGTAAGTCAATAACTTTAGCTTATTTATAAGGTTCAAGATCTTTAAATACTGTGGTCATTTTAGAGTTAATAAAAGTTCTTAAAAATTCTTTTTTTTATTGAAAGTTAAGCGCCTTTTTAAACATATTTAAAAAAATAAATAATGAATAAGAAGTCTTTCCTTCTTAATCTGTGGGCCCTCGGTCAACGATTACTATTGGCGCGCCATGGAATTGCGCAGCGAGAGAAGTAAGGTGGCAGGCGCGACCGGTCCGGCATTGCCCTATAGATAAAATCGATTCTTCCCACAATAACCACACGCGCCGGTGGCGAGAAAATCTAGATCTCAGTTATTGACAACTATGATTCGAGACATGGCATTGAAGTTCTTGAACAAGAAAGGATGGCACACTGGCAGTCTCCGGAATATAGAGAACGTGTGGAAGGCGGAGCAAAAGCATGAGGCGGAGCAGAAGAAGTTGGACGAACTCCGCAAGCAAATCCATGAGGAGAAGGAGCGTCAAGAGTTTCGTCAAGAGTTTCGTCTTCTCCAGAAAGAGAGTATGATTCTGAAAGTGGCAGCCCTGAGAGGCAGAGAAGGAATCATGATCGTGACAAGAGTTCAAAACGTGATGTTCGTTCTTATTCAGAAGATAGAAGAAGCAAAACTGATTATATGAATGAGAGAGATCGTGATAGAGGCTCAAAATATGATCAGTCAGATCGAAAGACGCCCAAAAGCAATCCTCATGATTATGCAGAAGAGAGACATCATGACAGATCAAAGAGAGACAGAGAGTCTTATACTCACAATGAAAAGGACAGTAGAAAAGTGGGGGAATCAAGAACTCGTGAACCGAATGCTTCTAAATCTCCTTACGAGCCACGCCATAAGCATCGTAGGCCTGATACCAAGCTTTCAGAAGAAGAGCGTGCAGCCAGGCTGCGTGAGATGCAGCAGGATGCTGAGTTCCATGAAGAGCAAAGATGGAGGCGTTTGAAGAAGGCAGCCGAGGATGACGCACTTGAAGCCGAACAAAATGCAGTGCCCTCGAGCCGAAATTTCTTAGACACTGCTCAGAAACACACGTATGGTGCCGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGTCGGAGGACATACTATTCACAGGGTAAATCTGAGATTGAAGCCAACGCTTTTAGACGGTAA

mRNA sequence

ATGTACCGATCGACGAGCTGGAACCGATTCTCCAATGAATACTACTCGCATTCGGCCCCGTCCTCGAAGTCAGGGCAGGCGTCGCGATTGTGCTTGTCCTTTGACGGCAATGAGCTGCCAATGAATGATCCGGTCGCCGAGATGATGAAGCGAGAGAAAGCGCGAGTCAAGTTCGCCGAATCTGCCGTTCATGTGATTCCGCTCGTTTTGCTTCTCTGCACCTTGTTTTCTGGTTCATCTCAAATCCAGTTCTTGAACAAGAAAGGATGGCACACTGGCAGTCTCCGGAATATAGAGAACGTGTGGAAGGCGGAGCAAAAGCATGAGGCGGAGCAGAAGAAGTTGGACGAACTCCGCAAGCAAATCCATGAGGAGAAGGAGCGTCAAGAGTTTCGTCAAGAGTTTCGTCTTCTCCAGAAAGAGAAAGATAGAAGAAGCAAAACTGATTATATGAATGAGAGAGATCGTGATAGAGGCTCAAAATATGATCAGTCAGATCGAAAGACGCCCAAAAGCAATCCTCATGATTATGCAGAAGAGAGACATCATGACAGATCAAAGAGAGACAGAGAGTCTTATACTCACAATGAAAAGGACAGTAGAAAAGTGGGGGAATCAAGAACTCGTGAACCGAATGCTTCTAAATCTCCTTACGAGCCACGCCATAAGCATCGTAGGCCTGATACCAAGCTTTCAGAAGAAGAGCGTGCAGCCAGGCTGCGTGAGATGCAGCAGGATGCTGAGTTCCATGAAGAGCAAAGATGGAGGCGTTTGAAGAAGGCAGCCGAGGATGACGCACTTGAAGCCGAACAAAATGCAGTGCCCTCGAGCCGAAATTTCTTAGACACTGCTCAGAAACACACGTATGGTGCCGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGTCGGAGGACATACTATTCACAGGGTAAATCTGAGATTGAAGCCAACGCTTTTAGACGGTAA

Coding sequence (CDS)

ATGTACCGATCGACGAGCTGGAACCGATTCTCCAATGAATACTACTCGCATTCGGCCCCGTCCTCGAAGTCAGGGCAGGCGTCGCGATTGTGCTTGTCCTTTGACGGCAATGAGCTGCCAATGAATGATCCGGTCGCCGAGATGATGAAGCGAGAGAAAGCGCGAGTCAAGTTCGCCGAATCTGCCGTTCATGTGATTCCGCTCGTTTTGCTTCTCTGCACCTTGTTTTCTGGTTCATCTCAAATCCAGTTCTTGAACAAGAAAGGATGGCACACTGGCAGTCTCCGGAATATAGAGAACGTGTGGAAGGCGGAGCAAAAGCATGAGGCGGAGCAGAAGAAGTTGGACGAACTCCGCAAGCAAATCCATGAGGAGAAGGAGCGTCAAGAGTTTCGTCAAGAGTTTCGTCTTCTCCAGAAAGAGAAAGATAGAAGAAGCAAAACTGATTATATGAATGAGAGAGATCGTGATAGAGGCTCAAAATATGATCAGTCAGATCGAAAGACGCCCAAAAGCAATCCTCATGATTATGCAGAAGAGAGACATCATGACAGATCAAAGAGAGACAGAGAGTCTTATACTCACAATGAAAAGGACAGTAGAAAAGTGGGGGAATCAAGAACTCGTGAACCGAATGCTTCTAAATCTCCTTACGAGCCACGCCATAAGCATCGTAGGCCTGATACCAAGCTTTCAGAAGAAGAGCGTGCAGCCAGGCTGCGTGAGATGCAGCAGGATGCTGAGTTCCATGAAGAGCAAAGATGGAGGCGTTTGAAGAAGGCAGCCGAGGATGACGCACTTGAAGCCGAACAAAATGCAGTGCCCTCGAGCCGAAATTTCTTAGACACTGCTCAGAAACACACGTATGGTGCCGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGTCGGAGGACATACTATTCACAGGGTAAATCTGAGATTGAAGCCAACGCTTTTAGACGGTAA

Protein sequence

MYRSTSWNRFSNEYYSHSAPSSKSGQASRLCLSFDGNELPMNDPVAEMMKREKARVKFAESAVHVIPLVLLLCTLFSGSSQIQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRQEFRLLQKEKDRRSKTDYMNERDRDRGSKYDQSDRKTPKSNPHDYAEERHHDRSKRDRESYTHNEKDSRKVGESRTREPNASKSPYEPRHKHRRPDTKLSEEERAARLREMQQDAEFHEEQRWRRLKKAAEDDALEAEQNAVPSSRNFLDTAQKHTYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR

Homology

[Close]

BLAST of Carg16767 vs. NCBI nr
Match: KAG7014853.1 (hypothetical protein SDJN02_22483, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605.5 bits (1560), Expect = 2.6e-169
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MYRSTSWNRFSNEYYSHSAPSSKSGQASRLCLSFDGNELPMNDPVAEMMKREKARVKFAE 60
           MYRSTSWNRFSNEYYSHSAPSSKSGQASRLCLSFDGNELPMNDPVAEMMKREKARVKFAE
Sbjct: 1   MYRSTSWNRFSNEYYSHSAPSSKSGQASRLCLSFDGNELPMNDPVAEMMKREKARVKFAE 60

Query: 61  SAVHVIPLVLLLCTLFSGSSQIQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRK 120
           SAVHVIPLVLLLCTLFSGSSQIQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRK
Sbjct: 61  SAVHVIPLVLLLCTLFSGSSQIQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRK 120

Query: 121 QIHEEKERQEFRQEFRLLQKEKDRRSKTDYMNERDRDRGSKYDQSDRKTPKSNPHDYAEE 180
           QIHEEKERQEFRQEFRLLQKEKDRRSKTDYMNERDRDRGSKYDQSDRKTPKSNPHDYAEE
Sbjct: 121 QIHEEKERQEFRQEFRLLQKEKDRRSKTDYMNERDRDRGSKYDQSDRKTPKSNPHDYAEE 180

Query: 181 RHHDRSKRDRESYTHNEKDSRKVGESRTREPNASKSPYEPRHKHRRPDTKLSEEERAARL 240
           RHHDRSKRDRESYTHNEKDSRKVGESRTREPNASKSPYEPRHKHRRPDTKLSEEERAARL
Sbjct: 181 RHHDRSKRDRESYTHNEKDSRKVGESRTREPNASKSPYEPRHKHRRPDTKLSEEERAARL 240

Query: 241 REMQQDAEFHEEQRWRRLKKAAEDDALEAEQNAVPSSRNFLDTAQKHTYGAEKGGSSTIE 300
           REMQQDAEFHEEQRWRRLKKAAEDDALEAEQNAVPSSRNFLDTAQKHTYGAEKGGSSTIE
Sbjct: 241 REMQQDAEFHEEQRWRRLKKAAEDDALEAEQNAVPSSRNFLDTAQKHTYGAEKGGSSTIE 300

Query: 301 ESIRRRTYYSQGKSEIEANAFRR 324
           ESIRRRTYYSQGKSEIEANAFRR
Sbjct: 301 ESIRRRTYYSQGKSEIEANAFRR 323

[Close]

BLAST of Carg16767 vs. NCBI nr
Match: XP_023551879.1 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-85
Identity = 221/411 (53.77%), Postives = 230/411 (55.96%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR---QEFRLL 141
           ++FLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR   ++  L+
Sbjct: 8   LKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAGLV 67

Query: 142 QKE--------------------------------------------------------- 201
            K+                                                         
Sbjct: 68  PKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAATEPSSSKEAAAPGALFEDK 127

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 128 PHSANDSWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRER 187

Query: 262 ------------------------------------------------KDRRSKTDYMNE 321
                                                           +DRRSKTDY+NE
Sbjct: 188 KKHRHSKSKRRKASSPERDYDSESVSPEMQRRNHDRDKSSKHDVHSYSEDRRSKTDYVNE 247

Query: 322 RDRDRGSKYDQSDRKTPKSNPHD-YAEERHHDRSKRDRESYTHNEKDSRKVGESRTREPN 324
           RDRDRGSKYDQSDRKTPKSNPHD YAEERHHD  KRDR+SYTHNEKDS KVGESRTREPN
Sbjct: 248 RDRDRGSKYDQSDRKTPKSNPHDYYAEERHHDSXKRDRDSYTHNEKDSTKVGESRTREPN 307

[Close]

BLAST of Carg16767 vs. NCBI nr
Match: XP_022922575.1 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog [Cucurbita moschata])

HSP 1 Score: 324.7 bits (831), Expect = 9.0e-85
Identity = 219/414 (52.90%), Postives = 230/414 (55.56%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR---QEFRLL 141
           ++FLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR   ++  L+
Sbjct: 3   LKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAGLV 62

Query: 142 QKE--------------------------------------------------------- 201
            K+                                                         
Sbjct: 63  PKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAAPEPSSSKEAAAPGALFEDK 122

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 123 PHSANDAWRKLHSDPLLIIRQREQXALARVKNNPIQMAMIRKTVEVEKHKAKNPDDEGER 182

Query: 262 ---------------------------------------------------KDRRSKTDY 321
                                                              +DRRSKTDY
Sbjct: 183 KKHCHSKSKRRKDSPPGREYDSESASPERQRRKRRNHDRDKSSKRDVRSYSEDRRSKTDY 242

Query: 322 MNERDRDRGSKYDQSDRKTPKSNPHD-YAEERHHDRSKRDRESYTHNEKDSRKVGESRTR 324
           MNERDRD GSKYDQSDRKTPKSNPHD YAEERHHD SKR R+SYTHNEKDSR+VGESRT 
Sbjct: 243 MNERDRDSGSKYDQSDRKTPKSNPHDYYAEERHHDSSKRYRDSYTHNEKDSRRVGESRTY 302

[Close]

BLAST of Carg16767 vs. NCBI nr
Match: XP_022984402.1 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog [Cucurbita maxima])

HSP 1 Score: 301.6 bits (771), Expect = 8.1e-78
Identity = 212/423 (50.12%), Postives = 224/423 (52.96%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR--------- 141
           ++FLNKKGWHT SL NIENVW+AEQKHEAEQKKLDELR+QIHEEKE QEFR         
Sbjct: 3   LKFLNKKGWHTDSLXNIENVWEAEQKHEAEQKKLDELREQIHEEKEXQEFRLLQDLAGLD 62

Query: 142 ----------------------QEFRLLQ------------------------------- 201
                                   F+ L+                               
Sbjct: 63  SKQKRLDFLYESGLAVGKGSNSDGFKSLETLPTTSTAPAATEPSSSKEAAAPGALFEDKP 122

Query: 202 ------------------------------------------------KEK--------- 261
                                                           K+K         
Sbjct: 123 HSANDAWRKLHSDPLLIIRQREQQALARVKNNPIPMAMIRKTVEAEKHKDKNPDDKRERM 182

Query: 262 ------------------------------------------------------------ 321
                                                                       
Sbjct: 183 KHRHSKSKRRKDSSPEREYDSESASPERQRRNHDSESASPERQRRNHDRDKSSKRDVRSY 242

Query: 322 --DRRSKTDYMNERDRDRGSKYDQSDRKTPKSNPHD-YAEERHHDRSKRDRESYTHNEKD 323
             DRRSKTDYMNERDRDRGSK+DQSDRKTPK NPHD YAEERHHD SKRDR+SYT NEKD
Sbjct: 243 SEDRRSKTDYMNERDRDRGSKFDQSDRKTPKRNPHDYYAEERHHDSSKRDRDSYTRNEKD 302

[Close]

BLAST of Carg16767 vs. NCBI nr
Match: XP_038896474.1 (pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida])

HSP 1 Score: 278.9 bits (712), Expect = 5.7e-71
Identity = 205/415 (49.40%), Postives = 220/415 (53.01%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR--------- 141
           ++FLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFR         
Sbjct: 3   LKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLV 62

Query: 142 --QE--------------------FRLLQ------------------------------- 201
             QE                    F+ L+                               
Sbjct: 63  PKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDKP 122

Query: 202 ------------------------------------------------KEK--------- 261
                                                           KEK         
Sbjct: 123 HSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKEKNPDGKRERK 182

Query: 262 ------------------------------------------------DRRSKTDYMNER 321
                                                           DRRSK D  NER
Sbjct: 183 KHRHSKSKRHKDSSSERDYDSEDASPERQRRKHDHENSSKHDAHSHLEDRRSKADSKNER 242

Query: 322 DRDRGSK------YDQSDRKTPKSNPHDYAEERHHDRSKRDRESYTHNEKDSRKVGESRT 324
           DRDRGSK      YDQSDR+T KSNPHD A ERHHDRS+RDR+SY +NEK+SR+VGE R+
Sbjct: 243 DRDRGSKYAARTTYDQSDRRTSKSNPHDSAAERHHDRSRRDRDSYANNEKNSRRVGEPRS 302

[Close]

BLAST of Carg16767 vs. ExPASy Swiss-Prot
Match: Q9NXE8 (Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 31/65 (47.69%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 87  KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRQEFRLLQKEKDRRS 146
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL++++ EE+ R+E ++    +   K +  
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 147 KTDYM 152
           K D+M
Sbjct: 69  KLDWM 73

[Close]

BLAST of Carg16767 vs. ExPASy Swiss-Prot
Match: Q9DBF7 (Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 31/65 (47.69%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 87  KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRQEFRLLQKEKDRRS 146
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL++++ EE+ R+E ++    +   K +  
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 147 KTDYM 152
           K D+M
Sbjct: 69  KLDWM 73

[Close]

BLAST of Carg16767 vs. ExPASy TrEMBL
Match: A0A6J1E963 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430542 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 4.4e-85
Identity = 219/414 (52.90%), Postives = 230/414 (55.56%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR---QEFRLL 141
           ++FLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR   ++  L+
Sbjct: 3   LKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAGLV 62

Query: 142 QKE--------------------------------------------------------- 201
            K+                                                         
Sbjct: 63  PKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAAPEPSSSKEAAAPGALFEDK 122

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 123 PHSANDAWRKLHSDPLLIIRQREQXALARVKNNPIQMAMIRKTVEVEKHKAKNPDDEGER 182

Query: 262 ---------------------------------------------------KDRRSKTDY 321
                                                              +DRRSKTDY
Sbjct: 183 KKHCHSKSKRRKDSPPGREYDSESASPERQRRKRRNHDRDKSSKRDVRSYSEDRRSKTDY 242

Query: 322 MNERDRDRGSKYDQSDRKTPKSNPHD-YAEERHHDRSKRDRESYTHNEKDSRKVGESRTR 324
           MNERDRD GSKYDQSDRKTPKSNPHD YAEERHHD SKR R+SYTHNEKDSR+VGESRT 
Sbjct: 243 MNERDRDSGSKYDQSDRKTPKSNPHDYYAEERHHDSSKRYRDSYTHNEKDSRRVGESRTY 302

[Close]

BLAST of Carg16767 vs. ExPASy TrEMBL
Match: A0A6J1J231 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita maxima OX=3661 GN=LOC111482710 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 3.9e-78
Identity = 212/423 (50.12%), Postives = 224/423 (52.96%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR--------- 141
           ++FLNKKGWHT SL NIENVW+AEQKHEAEQKKLDELR+QIHEEKE QEFR         
Sbjct: 3   LKFLNKKGWHTDSLXNIENVWEAEQKHEAEQKKLDELREQIHEEKEXQEFRLLQDLAGLD 62

Query: 142 ----------------------QEFRLLQ------------------------------- 201
                                   F+ L+                               
Sbjct: 63  SKQKRLDFLYESGLAVGKGSNSDGFKSLETLPTTSTAPAATEPSSSKEAAAPGALFEDKP 122

Query: 202 ------------------------------------------------KEK--------- 261
                                                           K+K         
Sbjct: 123 HSANDAWRKLHSDPLLIIRQREQQALARVKNNPIPMAMIRKTVEAEKHKDKNPDDKRERM 182

Query: 262 ------------------------------------------------------------ 321
                                                                       
Sbjct: 183 KHRHSKSKRRKDSSPEREYDSESASPERQRRNHDSESASPERQRRNHDRDKSSKRDVRSY 242

Query: 322 --DRRSKTDYMNERDRDRGSKYDQSDRKTPKSNPHD-YAEERHHDRSKRDRESYTHNEKD 323
             DRRSKTDYMNERDRDRGSK+DQSDRKTPK NPHD YAEERHHD SKRDR+SYT NEKD
Sbjct: 243 SEDRRSKTDYMNERDRDRGSKFDQSDRKTPKRNPHDYYAEERHHDSSKRDRDSYTRNEKD 302

[Close]

BLAST of Carg16767 vs. ExPASy TrEMBL
Match: A0A5A7TSZ0 (Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00360 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.0e-70
Identity = 201/417 (48.20%), Postives = 220/417 (52.76%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR---QEFRLL 141
           ++FLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFR   ++  L+
Sbjct: 3   LKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLV 62

Query: 142 QKE--------------------------------------------------------- 201
            K+                                                         
Sbjct: 63  PKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFEDKP 122

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 123 HSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRERK 182

Query: 262 -----------------------------------------------KDRRSKTDYMNER 321
                                                          +DRRSK D  NER
Sbjct: 183 KHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKNER 242

Query: 322 DRDRGSK------YDQSDRKTPKSNPHDYAEERHHDRSK--RDRESYTHNEKDSRKVGES 324
           DRDRGSK      YDQSDRKT KSNP D A +RHHDRSK  RDR+S+ +N+KDSR+VGE 
Sbjct: 243 DRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVGEP 302

[Close]

BLAST of Carg16767 vs. ExPASy TrEMBL
Match: A0A1S3BY15 (pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.0e-70
Identity = 201/417 (48.20%), Postives = 220/417 (52.76%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR---QEFRLL 141
           ++FLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFR   ++  L+
Sbjct: 3   LKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLV 62

Query: 142 QKE--------------------------------------------------------- 201
            K+                                                         
Sbjct: 63  PKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFEDKP 122

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 123 HSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRERK 182

Query: 262 -----------------------------------------------KDRRSKTDYMNER 321
                                                          +DRRSK D  NER
Sbjct: 183 KHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKNER 242

Query: 322 DRDRGSK------YDQSDRKTPKSNPHDYAEERHHDRSK--RDRESYTHNEKDSRKVGES 324
           DRDRGSK      YDQSDRKT KSNP D A +RHHDRSK  RDR+S+ +N+KDSR+VGE 
Sbjct: 243 DRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVGEP 302

[Close]

BLAST of Carg16767 vs. ExPASy TrEMBL
Match: A0A0A0KU62 (Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 2.2e-68
Identity = 196/415 (47.23%), Postives = 217/415 (52.29%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFR---QEFRLL 141
           ++FLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFR   ++  L+
Sbjct: 3   LKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLV 62

Query: 142 QKE--------------------------------------------------------- 201
            K+                                                         
Sbjct: 63  PKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDKP 122

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 123 HSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRERK 182

Query: 262 -----------------------------------------------KDRRSKTDYMNER 321
                                                          +DRRSK +  NER
Sbjct: 183 KHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKNER 242

Query: 322 DRDRGSK------YDQSDRKTPKSNPHDYAEERHHDRSKRDRESYTHNEKDSRKVGESRT 324
           DRDRGSK      YDQSDRKT KSNPHD A +R+HDRSKRDR+SY  N++DSR+VGE R 
Sbjct: 243 DRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYATNDRDSRRVGELRY 302

[Close]

BLAST of Carg16767 vs. TAIR 10
Match: AT2G44200.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 108.6 bits (270), Expect = 9.4e-24
Identity = 93/226 (41.15%), Postives = 131/226 (57.96%), Query Frame = 0


Query: 106 QKHEAEQKKLDELRKQIHEEKERQE-----FRQEFRLLQKEKDRRSKTDYMNERDRDRGS 165
           ++ E+     +  R  + +E +R+E     F ++   L  E  RR   D     D D   
Sbjct: 283 KRRESRDNHYERRRSDLDDESKRRESHDKHFERQRSDLDDEYKRRESQDKRRRSDID--- 342

Query: 166 KYDQSDRKTPKSNPHDYAEERHHDRSKR---DRESYTHNEKDSRKVGESRTREPNASKSP 225
             D+  R+  + N      E++ +RS +   +RE+     ++ +K    R  E   S  P
Sbjct: 343 --DEPKRRDARPN------EKYRNRSPKGGVERENLKSYGQEDKK----RKAEDLDSGKP 402

Query: 226 YEPRHKHRRPDTKLSEEERAARLREMQQDAEFHEEQRWRRLKKAAEDDALEAEQNAVPSS 285
            E +++ R+  +KLSEEERAARL++MQ DAE HEEQRW RLKKA E DA+EA++N V + 
Sbjct: 403 NEYQNRRRKGGSKLSEEERAARLKQMQMDAEVHEEQRWTRLKKADETDAVEADKNKVSTG 462

Query: 286 RNFLDTAQKHTYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 324
           ++FLD A K  YG EKGGSSTIEES+RRR+YYSQ  +  E NAFRR
Sbjct: 463 KSFLDDANKSVYGVEKGGSSTIEESVRRRSYYSQRGTAAEGNAFRR 493

[Close]

BLAST of Carg16767 vs. TAIR 10
Match: AT5G61630.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G07490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 65.1 bits (157), Expect = 1.2e-10
Identity = 39/76 (51.32%), Postives = 48/76 (63.16%), Query Frame = 0


Query: 1  MYRSTSWNRFSNEYYSHSAPSSKSGQASRLCLSFDGNELP-MNDPVAEMMKREKARVKFA 60
          MYRS SWNR   +Y   SAP       S +    D NELP  N+P  EM+K+EK+R KFA
Sbjct: 1  MYRSASWNRVPEDY--SSAPPKGLWMGS-VIGPLDENELPSYNNPPEEMVKKEKSRAKFA 60

Query: 61 ESAVHVIPLVLLLCTL 76
          E+A+H+IP VLL C L
Sbjct: 61 ENAIHIIPFVLLACAL 73

[Close]

BLAST of Carg16767 vs. TAIR 10
Match: AT5G07490.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G61630.1); Has 50 Blast hits to 50 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 50; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 57.8 bits (138), Expect = 1.9e-08
Identity = 37/77 (48.05%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 1  MYRSTSWNRFSNEY-YSHSAPSSKSGQASRLCLSFDGNELPMNDPVA-EMMKREKARVKF 60
          MYRS SWNR + +Y    SAP         L    D +E    DP   EM K+EK+R KF
Sbjct: 1  MYRSASWNRVTEDYSVPWSAPKG-------LWKGLDEDEPAPYDPTGQEMTKKEKSRTKF 60

Query: 61 AESAVHVIPLVLLLCTL 76
          AE+AVH+IP VLL C L
Sbjct: 61 AENAVHIIPFVLLACAL 70

[Close]

BLAST of Carg16767 vs. TAIR 10
Match: AT2G44195.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 53.5 bits (127), Expect = 3.6e-07
Identity = 42/103 (40.78%), Postives = 60/103 (58.25%), Query Frame = 0


Query: 82  IQFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDE------LRKQ---IHEEKERQEFR 141
           ++FLNKKGWHTGS+RN+E VWKAEQKHEAEQ+K++E      +++Q   +    ER EF 
Sbjct: 3   LKFLNKKGWHTGSIRNVEKVWKAEQKHEAEQEKIEERSEFRAIQEQAGLVPRRPERLEFL 62

Query: 142 QEFRLL---QKEKDRRSKTDYMNERDRDR----GSKYDQSDRK 169
            +  L    Q     R    + N+  R R    G+ +D  D++
Sbjct: 63  YDPELAVAKQNVSSSRHGVLFQNQDQRARATIPGALFDDDDKR 105

The following BLAST results are available for this feature:

BLAST of Carg16767 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014853.1	2.6e-169	100.00	hypothetical protein SDJN02_22483, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023551879.1	1.1e-85	53.77	LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita ...	[more]
XP_022922575.1	9.0e-85	52.90	LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog [Cucurbita moschata]	[more]
XP_022984402.1	8.1e-78	50.12	LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog [Cucurbita maxima]	[more]
XP_038896474.1	5.7e-71	49.40	pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida]	[more]

BLAST of Carg16767 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9NXE8	1.2e-07	47.69	Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=...	[more]
Q9DBF7	1.2e-07	47.69	Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV...	[more]

BLAST of Carg16767 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E963	4.4e-85	52.90	LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschat...	[more]
A0A6J1J231	3.9e-78	50.12	LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita maxima ...	[more]
A0A5A7TSZ0	1.0e-70	48.20	Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BY15	1.0e-70	48.20	pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 P...	[more]
A0A0A0KU62	2.2e-68	47.23	Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 ...	[more]

BLAST of Carg16767 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44200.1	9.4e-24	41.15	CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor	[more]
AT5G61630.1	1.2e-10	51.32	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G07490.1	1.9e-08	48.05	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT2G44195.1	3.6e-07	40.78	CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 101..128
None	No IPR available	COILS	Coil	Coil	coord: 252..272
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 137..254
None	No IPR available	PANTHER	PTHR16196	CELL CYCLE CONTROL PROTEIN CWF25	coord: 142..323 coord: 83..133
IPR019339	CBF1-interacting co-repressor CIR, N-terminal domain	SMART	SM01083	Cir_N_3	coord: 89..125 e-value: 2.3E-12 score: 57.1
IPR019339	CBF1-interacting co-repressor CIR, N-terminal domain	PFAM	PF10197	Cir_N	coord: 90..125 e-value: 9.5E-15 score: 54.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16767-RA	Carg16767-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006397	mRNA processing
cellular_component	GO:0005634	nucleus