Carg16696 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16696
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerases II and V subunit 6B-like
Location	Carg_Chr16: 728369 .. 731067 (+)
RNA-Seq Expression	Carg16696
Synteny	Carg16696

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTGGTCGTTTACTTCTGAAGTTTTGCAACTGAGGGTTTTAGTGAGTAACCAGAGAGCGCGGCAACAGTTTCTCCATCGAACATCAATGGCGGACGATGATTACAATGACCTCGACATGGGGTTCGATCTCTTTCCATATGCTTTTATTTATTCAATATGTTCTTCTCGAATCTTTTAATGTTCCTTTGGTCAATTGTTCTCTTTGAAAATCCGTCGAATGAAAAAGCCTTTCGAAACTAAGTTCGTTCCTTCAAGTTCAACTCGGTTAGTACGGATTTGGAACTGAATTTGTGAAGGAAAATAGTTCGGTTATGATGAAGATTATTTAGCTGTTTAGAAGGAGATGCTATCACGTACTGGAACCAGACTCGGTTGTGCGAGAGATTCTGCACAGTCTGCTTTTATGTAGTTATAAGCGTGTGAGGAGTTTAAAAGAGTTACTTCGGCAAATGTTTGATTTCTCTGGCTGCCTTACAGCTCACAATACCAATGTTTAAAGGTTTAAGCGTGTGCAATGATCTTAGATAGCTGCTTGTGACATATTCAATTAGGGTTTTTCTTTAGGTATAAATTATGGACTTCTTCTGCCTTGAAAGCATGGCTGCTGGACAAACGTTACTCAAAATTTCTACTTCTTTTTCCTTCATAATCAGATACCAAATTAAGTTTGTAACTGCCACTCGTCATTGTAATATTCCGGAAACTACAGTCTAAGCCCTGAGATGTGAAAAGATGTTTACGACCTTATGCTCCTTATTGGATACTTATGTTCATAAGCATCATGCTTTAGTGAACTACGTTATTTTAGGTCAGTTTTGCTAATTGTTGGGCAATACACGTTTTATTCATTAGTGAAAAGGAACTTTGATAACCTTCATTTTTATAATGGATTACTATGTCTAAGATTTAAACATTGTATGTTGAAGAATTGTTTTTAAAGCTTTCCTGTTAGTAGTTTATTTAATTGGATAGGCCTCTTGCTGCATATATACATTAAATCTTGTATTATCAGTTCTTCAAAGTAGGCTTTTATTCACTTTATTCCCTGTTTCTGATGTCCCTTTTTATTTGCTTGAAGAACCGGCCCTGCTTCTAGAATACAGTTAGCTCATCAGATTGCTGTACCAGCTGCATTTTTTTAAAAAAAATATCTGTTAACTTAGACTGATAGACCGTGTTTAGTGTTTTATGTCACATTTTACGTCACATTTTTTGGATTTTATATCTGTGGTTGAGGACTGGAGATCTGAATTTTGGGGCTACACAAATGGTTTGCTATTAGAAGTTAATATAATAAGATCCCATTCTTAGCTTTAGTTTGACCACACTGGTATAGTCTTGTTTTGTGAAAGTCCTTTAGATTTTCATCTAGGCTGATTGGGAGGTTCAAATATCTTTCAGCTTTTCTATTAATTTAGTGGTTTTTGTTGCAGATATGACGATGAGCCACCAGAACCAGAATTGGAGGTATGAAATAATTAATTCAACTTTATTTAAAAGAAAGTAGATAACTGTTCTTTTTGAAGTTGATTAATTATAGTGGAACTGAAAATGATGTACAGGAAGGTGCTGAAGAAGAATTGGATAATAATAACAATGATGATGTTCCTGGTGAACCTGTTGAAGCAGAGGAAAAGGAAGATGAAGAACCTGTTGAACGTGTGCGTAAAACCTCAAAATTTATGACAAAGTATGAACGTGCTAGAATTTTGGGTACACGTGCCCTCCAAATCAGGTTCTCTTTTTAACATTTGCTACATCCAAATTTTTTACGGGCATCAAGTTGATAATTGGTATGATGGTTCTTTTTCCTATTGGGCAGCATGAATGCTCCTGTGATGGTCGAATTGGAGGGCGAGACTGATCCACTTGAGGTTAGATTTTTTAAAATGTTAAGCTAAGATCTTTGGAAAGCTCTCTTTTGGTTTGTGCATCCAGCTAGATGTATTTTTGTTGCCTCATATGTGCATGCCATGGTTAGTATGCCTACTCAAAAGAAAAGGGTTAAATTTAAATCATACAAAAATGCCCTCAAACTTTGAGGTTGGGGGTTAAATTTGAACTATTTCAAACAATAGAGTATAATTGAAACTAGCATCAAAGCTTCAGTAAGTTAGTCAAACTGATTTGACCGCTTACAGTTTGTAATTGTAATAGCTCTGCTGGTATAGTGAATGTGGATTTATATGCTTATCCATTTGAGTGATTCGCATGTTCTTGGTTGTTTGTGGCGTTGGTCTTTCATATCTCATCTAATCCTGTGCTTTTTAGATTGCAATGAAGGAGCTTCGTGAGCGGAAGATACCCTTCACCATCCGTCGCTACCTGCCAGATGGAAGGTTAGAGCTTTTAGATCTTATTCTTTTATCTTTTAACATTTTATTGCCTGAAGGGGGGTGGGGGGTGCAGAAAATTGAAAACAAGTGTAACCAACTGATTTGGTTTCTTATGTGTTCATGTATGTATAAGTTTAAAGAGACCGGGTTTTGGACTAGTTTTAGCGAAAAAGAAATTTTCCTAAGGTTTTGGTTCTCGCAATTTTCCAATACCAAAATCATTCTAGTTTTCTGTAGGATAAGATAAAGAATGGTACTTGAGTGAAACTTTCTGTCTGTTAATTCTGCGCAGTTATGAAGACTGGGGCGTTGATGAACTGATTGTGGAGGACTCCTGGAAGAGGCAAGTTGGTGGCGCTTGA

mRNA sequence

CGTGGTCGTTTACTTCTGAAGTTTTGCAACTGAGGGTTTTAGTGAGTAACCAGAGAGCGCGGCAACAGTTTCTCCATCGAACATCAATGGCGGACGATGATTACAATGACCTCGACATGGGATATGACGATGAGCCACCAGAACCAGAATTGGAGGAAGGTGCTGAAGAAGAATTGGATAATAATAACAATGATGATGTTCCTGGTGAACCTGTTGAAGCAGAGGAAAAGGAAGATGAAGAACCTGTTGAACGTGTGCGTAAAACCTCAAAATTTATGACAAAGTATGAACGTGCTAGAATTTTGGGTACACGTGCCCTCCAAATCAGCATGAATGCTCCTGTGATGGTCGAATTGGAGGGCGAGACTGATCCACTTGAGATTGCAATGAAGGAGCTTCGTGAGCGGAAGATACCCTTCACCATCCGTCGCTACCTGCCAGATGGAAGTTATGAAGACTGGGGCGTTGATGAACTGATTGTGGAGGACTCCTGGAAGAGGCAAGTTGGTGGCGCTTGA

Coding sequence (CDS)

ATGGCGGACGATGATTACAATGACCTCGACATGGGATATGACGATGAGCCACCAGAACCAGAATTGGAGGAAGGTGCTGAAGAAGAATTGGATAATAATAACAATGATGATGTTCCTGGTGAACCTGTTGAAGCAGAGGAAAAGGAAGATGAAGAACCTGTTGAACGTGTGCGTAAAACCTCAAAATTTATGACAAAGTATGAACGTGCTAGAATTTTGGGTACACGTGCCCTCCAAATCAGCATGAATGCTCCTGTGATGGTCGAATTGGAGGGCGAGACTGATCCACTTGAGATTGCAATGAAGGAGCTTCGTGAGCGGAAGATACCCTTCACCATCCGTCGCTACCTGCCAGATGGAAGTTATGAAGACTGGGGCGTTGATGAACTGATTGTGGAGGACTCCTGGAAGAGGCAAGTTGGTGGCGCTTGA

Protein sequence

MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKTSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDGSYEDWGVDELIVEDSWKRQVGGA

Homology

[Close]

BLAST of Carg16696 vs. NCBI nr
Match: XP_022923151.1 (DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita moschata] >XP_022984652.1 DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita maxima] >XP_022984654.1 DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita maxima] >XP_023551729.1 DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita pepo subsp. pepo] >KAG6576740.1 DNA-directed RNA polymerases II, IV and V subunit 6A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014784.1 DNA-directed RNA polymerases II, IV and V subunit 6A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 280.8 bits (717), Expect = 6.6e-72
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT
Sbjct: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. NCBI nr
Match: XP_022141284.1 (DNA-directed RNA polymerases II, IV and V subunit 6A [Momordica charantia])

HSP 1 Score: 274.6 bits (701), Expect = 4.7e-70
Identity = 140/143 (97.90%), Postives = 142/143 (99.30%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYNDLDMGY+DEPPEPE+EEGAEEELDNNNNDDV GEPVEAEEKEDEEPVERVRKT
Sbjct: 1   MADDDYNDLDMGYEDEPPEPEMEEGAEEELDNNNNDDVLGEPVEAEEKEDEEPVERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. NCBI nr
Match: XP_022944842.1 (DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita moschata] >XP_022968530.1 DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita maxima] >XP_023541080.1 DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita pepo subsp. pepo] >KAG7013212.1 DNA-directed RNA polymerases II, IV and V subunit 6A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 272.7 bits (696), Expect = 1.8e-69
Identity = 137/143 (95.80%), Postives = 142/143 (99.30%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYND+DMGY+DEPPEPE+EEGAEEELDNNN DDVPGEPVEAE+KEDEEP+ERVRKT
Sbjct: 1   MADDDYNDVDMGYEDEPPEPEMEEGAEEELDNNNADDVPGEPVEAEDKEDEEPIERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. NCBI nr
Match: XP_004140788.1 (DNA-directed RNA polymerases II, IV and V subunit 6A [Cucumis sativus] >KGN57349.1 hypothetical protein Csa_009934 [Cucumis sativus])

HSP 1 Score: 271.9 bits (694), Expect = 3.1e-69
Identity = 136/143 (95.10%), Postives = 141/143 (98.60%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYNDLDMGY+DEPPEPE+EEGAEEEL+NNNNDD+PGEPVEAEEKEDEEP+ R RKT
Sbjct: 1   MADDDYNDLDMGYEDEPPEPEIEEGAEEELENNNNDDIPGEPVEAEEKEDEEPIVRARKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. NCBI nr
Match: XP_008439178.1 (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 6A [Cucumis melo])

HSP 1 Score: 271.2 bits (692), Expect = 5.2e-69
Identity = 137/143 (95.80%), Postives = 140/143 (97.90%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYNDLDMGY+DEPPEPE+EEGAEEELDN NNDD+ GEPVEAEEKEDEEPVER RKT
Sbjct: 1   MADDDYNDLDMGYEDEPPEPEIEEGAEEELDNTNNDDITGEPVEAEEKEDEEPVERARKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. ExPASy Swiss-Prot
Match: Q9FJ98 (DNA-directed RNA polymerases II, IV and V subunit 6A OS=Arabidopsis thaliana OX=3702 GN=NRPB6A PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 2.1e-60
Identity = 118/143 (82.52%), Postives = 133/143 (93.01%), Query Frame = 0


Query: 1   MADDDYNDL-DMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRK 60
           MAD+DYND+ D+GY+DEP EPE+EEG EE+++   NDDV GEP+EAE+K + EPV+R RK
Sbjct: 1   MADEDYNDVDDLGYEDEPAEPEIEEGVEEDVEMKENDDVNGEPIEAEDKVETEPVQRPRK 60

Query: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPD 120
           TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELR+RKIPFTIRRYLPD
Sbjct: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRQRKIPFTIRRYLPD 120

Query: 121 GSYEDWGVDELIVEDSWKRQVGG 143
           GS+E+WGVDELIVEDSWKRQVGG
Sbjct: 121 GSFEEWGVDELIVEDSWKRQVGG 143

[Close]

BLAST of Carg16696 vs. ExPASy Swiss-Prot
Match: Q9SJ96 (DNA-directed RNA polymerases II and V subunit 6B OS=Arabidopsis thaliana OX=3702 GN=NRPB6B PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 7.3e-58
Identity = 116/143 (81.12%), Postives = 129/143 (90.21%), Query Frame = 0


Query: 1   MADDDYNDL-DMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRK 60
           MADDDYN++ D+GY+DEP EPE+EEG EE+ D   NDDV  +P+E E+K + EPV+R RK
Sbjct: 1   MADDDYNEVDDLGYEDEPAEPEIEEGVEEDADIKENDDVNVDPLETEDKVETEPVQRPRK 60

Query: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPD 120
           TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELR+RKIPFTIRRYLPD
Sbjct: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRQRKIPFTIRRYLPD 120

Query: 121 GSYEDWGVDELIVEDSWKRQVGG 143
            SYE+WGVDELIVEDSWKRQVGG
Sbjct: 121 MSYEEWGVDELIVEDSWKRQVGG 143

[Close]

BLAST of Carg16696 vs. ExPASy Swiss-Prot
Match: P61219 (DNA-directed RNA polymerases I, II, and III subunit RPABC2 OS=Mus musculus OX=10090 GN=Polr2f PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.2e-29
Identity = 80/133 (60.15%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 4   DDYNDL--DMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKTS 63
           DD++D+  D G DD        E AEEE   N      GE  +A +K         R T+
Sbjct: 11  DDFDDVEEDEGLDD-------LENAEEEGQENVEILPSGERPQANQK---------RITT 70

Query: 64  KFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDGS 123
            +MTKYERAR+LGTRALQI+M APVMVELEGETDPL IAMKEL+ RKIP  IRRYLPDGS
Sbjct: 71  PYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGS 127

Query: 124 YEDWGVDELIVED 135
           YEDWGVDELI+ D
Sbjct: 131 YEDWGVDELIISD 127

[Close]

BLAST of Carg16696 vs. ExPASy Swiss-Prot
Match: O88828 (DNA-directed RNA polymerases I, II, and III subunit RPABC2 OS=Rattus norvegicus OX=10116 GN=Polr2f PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.2e-29
Identity = 80/133 (60.15%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 4   DDYNDL--DMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKTS 63
           DD++D+  D G DD        E AEEE   N      GE  +A +K         R T+
Sbjct: 11  DDFDDVEEDEGLDD-------LENAEEEGQENVEILPSGERPQANQK---------RITT 70

Query: 64  KFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDGS 123
            +MTKYERAR+LGTRALQI+M APVMVELEGETDPL IAMKEL+ RKIP  IRRYLPDGS
Sbjct: 71  PYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGS 127

Query: 124 YEDWGVDELIVED 135
           YEDWGVDELI+ D
Sbjct: 131 YEDWGVDELIISD 127

[Close]

BLAST of Carg16696 vs. ExPASy Swiss-Prot
Match: P61217 (DNA-directed RNA polymerases I, II, and III subunit RPABC2 OS=Cricetulus griseus OX=10029 GN=POLR2F PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 9.4e-29
Identity = 80/133 (60.15%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 4   DDYNDL--DMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKTS 63
           DD++D+  D G DD        E AEEE   N      GE  +A +K         R T+
Sbjct: 11  DDFDDVEEDEGLDD-------LENAEEEGQENVEILPSGERPQANQK---------RITT 70

Query: 64  KFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDGS 123
            +MTKYERAR+LGTRALQI+M APVMVELEGETDPL IAMKEL+ RKIP  IRRYLPDGS
Sbjct: 71  PYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGS 127

Query: 124 YEDWGVDELIVED 135
           YEDWGVDELI+ D
Sbjct: 131 YEDWGVDELIITD 127

[Close]

BLAST of Carg16696 vs. ExPASy TrEMBL
Match: A0A6J1J2R7 (DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita maxima OX=3661 GN=LOC111482872 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 3.2e-72
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT
Sbjct: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. ExPASy TrEMBL
Match: A0A6J1E614 (DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita moschata OX=3662 GN=LOC111430903 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 3.2e-72
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT
Sbjct: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. ExPASy TrEMBL
Match: A0A6J1CI61 (DNA-directed RNA polymerases II, IV and V subunit 6A OS=Momordica charantia OX=3673 GN=LOC111011721 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.3e-70
Identity = 140/143 (97.90%), Postives = 142/143 (99.30%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYNDLDMGY+DEPPEPE+EEGAEEELDNNNNDDV GEPVEAEEKEDEEPVERVRKT
Sbjct: 1   MADDDYNDLDMGYEDEPPEPEMEEGAEEELDNNNNDDVLGEPVEAEEKEDEEPVERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. ExPASy TrEMBL
Match: A0A6J1HXF2 (DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita maxima OX=3661 GN=LOC111467735 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 8.7e-70
Identity = 137/143 (95.80%), Postives = 142/143 (99.30%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYND+DMGY+DEPPEPE+EEGAEEELDNNN DDVPGEPVEAE+KEDEEP+ERVRKT
Sbjct: 1   MADDDYNDVDMGYEDEPPEPEMEEGAEEELDNNNADDVPGEPVEAEDKEDEEPIERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. ExPASy TrEMBL
Match: A0A6J1FZ93 (DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita moschata OX=3662 GN=LOC111449252 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 8.7e-70
Identity = 137/143 (95.80%), Postives = 142/143 (99.30%), Query Frame = 0


Query: 1   MADDDYNDLDMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRKT 60
           MADDDYND+DMGY+DEPPEPE+EEGAEEELDNNN DDVPGEPVEAE+KEDEEP+ERVRKT
Sbjct: 1   MADDDYNDVDMGYEDEPPEPEMEEGAEEELDNNNADDVPGEPVEAEDKEDEEPIERVRKT 60

Query: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120
           SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG
Sbjct: 61  SKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDG 120

Query: 121 SYEDWGVDELIVEDSWKRQVGGA 144
           SYEDWGVDELIVEDSWKRQVGGA
Sbjct: 121 SYEDWGVDELIVEDSWKRQVGGA 143

[Close]

BLAST of Carg16696 vs. TAIR 10
Match: AT5G51940.1 (RNA polymerase Rpb6 )

HSP 1 Score: 233.0 bits (593), Expect = 1.5e-61
Identity = 118/143 (82.52%), Postives = 133/143 (93.01%), Query Frame = 0


Query: 1   MADDDYNDL-DMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRK 60
           MAD+DYND+ D+GY+DEP EPE+EEG EE+++   NDDV GEP+EAE+K + EPV+R RK
Sbjct: 1   MADEDYNDVDDLGYEDEPAEPEIEEGVEEDVEMKENDDVNGEPIEAEDKVETEPVQRPRK 60

Query: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPD 120
           TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELR+RKIPFTIRRYLPD
Sbjct: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRQRKIPFTIRRYLPD 120

Query: 121 GSYEDWGVDELIVEDSWKRQVGG 143
           GS+E+WGVDELIVEDSWKRQVGG
Sbjct: 121 GSFEEWGVDELIVEDSWKRQVGG 143

[Close]

BLAST of Carg16696 vs. TAIR 10
Match: AT2G04630.1 (RNA polymerase Rpb6 )

HSP 1 Score: 224.6 bits (571), Expect = 5.2e-59
Identity = 116/143 (81.12%), Postives = 129/143 (90.21%), Query Frame = 0


Query: 1   MADDDYNDL-DMGYDDEPPEPELEEGAEEELDNNNNDDVPGEPVEAEEKEDEEPVERVRK 60
           MADDDYN++ D+GY+DEP EPE+EEG EE+ D   NDDV  +P+E E+K + EPV+R RK
Sbjct: 1   MADDDYNEVDDLGYEDEPAEPEIEEGVEEDADIKENDDVNVDPLETEDKVETEPVQRPRK 60

Query: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPD 120
           TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELR+RKIPFTIRRYLPD
Sbjct: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRQRKIPFTIRRYLPD 120

Query: 121 GSYEDWGVDELIVEDSWKRQVGG 143
            SYE+WGVDELIVEDSWKRQVGG
Sbjct: 121 MSYEEWGVDELIVEDSWKRQVGG 143

The following BLAST results are available for this feature:

BLAST of Carg16696 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923151.1	6.6e-72	100.00	DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita moschata] >...	[more]
XP_022141284.1	4.7e-70	97.90	DNA-directed RNA polymerases II, IV and V subunit 6A [Momordica charantia]	[more]
XP_022944842.1	1.8e-69	95.80	DNA-directed RNA polymerases II, IV and V subunit 6A-like [Cucurbita moschata] >...	[more]
XP_004140788.1	3.1e-69	95.10	DNA-directed RNA polymerases II, IV and V subunit 6A [Cucumis sativus] >KGN57349...	[more]
XP_008439178.1	5.2e-69	95.80	PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 6A [Cucumis melo]	[more]

BLAST of Carg16696 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJ98	2.1e-60	82.52	DNA-directed RNA polymerases II, IV and V subunit 6A OS=Arabidopsis thaliana OX=...	[more]
Q9SJ96	7.3e-58	81.12	DNA-directed RNA polymerases II and V subunit 6B OS=Arabidopsis thaliana OX=3702...	[more]
P61219	7.2e-29	60.15	DNA-directed RNA polymerases I, II, and III subunit RPABC2 OS=Mus musculus OX=10...	[more]
O88828	7.2e-29	60.15	DNA-directed RNA polymerases I, II, and III subunit RPABC2 OS=Rattus norvegicus ...	[more]
P61217	9.4e-29	60.15	DNA-directed RNA polymerases I, II, and III subunit RPABC2 OS=Cricetulus griseus...	[more]

BLAST of Carg16696 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J2R7	3.2e-72	100.00	DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita maxima OX...	[more]
A0A6J1E614	3.2e-72	100.00	DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita moschata ...	[more]
A0A6J1CI61	2.3e-70	97.90	DNA-directed RNA polymerases II, IV and V subunit 6A OS=Momordica charantia OX=3...	[more]
A0A6J1HXF2	8.7e-70	95.80	DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita maxima OX...	[more]
A0A6J1FZ93	8.7e-70	95.80	DNA-directed RNA polymerases II, IV and V subunit 6A-like OS=Cucurbita moschata ...	[more]

BLAST of Carg16696 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51940.1	1.5e-61	82.52	RNA polymerase Rpb6	[more]
AT2G04630.1	5.2e-59	81.12	RNA polymerase Rpb6	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006110	RNA polymerase, subunit omega/K/RPB6	SMART	SM01409	RNA_pol_Rpb6_2	coord: 58..111 e-value: 4.7E-6 score: 36.1
IPR006110	RNA polymerase, subunit omega/K/RPB6	PFAM	PF01192	RNA_pol_Rpb6	coord: 60..111 e-value: 6.0E-16 score: 58.1
IPR006111	Archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit	PIRSF	PIRSF000778	RpoK/RPB6	coord: 1..134 e-value: 9.8E-49 score: 162.9
IPR006111	Archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit	HAMAP	MF_00192	RNApol_arch_K	coord: 62..117 score: 18.504309
IPR012293	RNA polymerase subunit, RPB6/omega	GENE3D	3.90.940.10		coord: 3..134 e-value: 1.1E-50 score: 172.8
IPR028363	DNA-directed RNA polymerase, subunit RPB6	PIRSF	PIRSF500154	RPB6	coord: 1..134 e-value: 9.9E-56 score: 185.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..60
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..50
None	No IPR available	PANTHER	PTHR10773:SF16	DNA-DIRECTED RNA POLYMERASES II AND V SUBUNIT 6B	coord: 1..142
None	No IPR available	PANTHER	PTHR10773	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2	coord: 1..142
IPR020708	DNA-directed RNA polymerase, 14-18kDa subunit, conserved site	PROSITE	PS01111	RNA_POL_K_14KD	coord: 65..79
IPR036161	RPB6/omega subunit-like superfamily	SUPERFAMILY	63562	RPB6/omega subunit-like	coord: 1..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16696-RA	Carg16696-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005768	endosome
cellular_component	GO:0016020	membrane
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0035091	phosphatidylinositol binding