Carg16550 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16550
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionLong-chain-alcohol oxidase
LocationCarg_Chr14: 13117384 .. 13120267 (+)
RNA-Seq ExpressionCarg16550
SyntenyCarg16550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATCCCTCGTCTCGGAGCAGAGGCATATTCATATTCAATCAATCTACAGAGCGACGATGCGGAGATCGGAGTGCCATCCCCTGTTGAGAGGAACAAGGAGCGGCAAGTTCACGCATGGCTTCTCCGTGGCGGAGATGGAGTCTTTAGCCACCATCTGCGACACTATATTGCCTCCTCTGCCGCTCGGTTCATCAAACAACAACGGCAGCGATGAAATCGATACTGCCGATGCGGCCGCAGTCGAGGCGTTTTACGCGGCGTCCGGTTCGCGATCTCCGATTCCGGATGAGGTGAGTTTAGTGACGGAAAAAGATGTGGATATTGTTCTTTGATTCGATGTGGATTTTAAGTTAGGTTTATGGAAGTTGAAATTAGGTTGCAGAGACAGTCGCGAAAAGAGGATTGACTGAACTCGTGATACTGGTGCGGATGGTGCTGTGGTTGCTAGGTACGAGGCTAGGGACGTTGCTGCTCTGTGGATCGCTCTGTTTTTGCAGGACATGGCCTTATATCTGCAATTTCTCGAGTATGCCATTGAAGAACAGAGAGGAAGCTTTGAGGAGATGGTATAAGAATAAGGTTTTCACGCCCATTAGAGCTGCTCTTGCTTCCATCAGAGTTCTTGTCCTCTACACCTTCTTTTCTCGGGTAATCTCTCCATCTCTCTCTACAATTTTTTTTTTTTTTAAATTTTCAAAAACACTATTTTTTTTTTTAATTAAAAAAAAAAAGAGAGAATATATGGAATTATTGAATAAAATGTAAATGTATGAGTATTATTAGGAAAATGCAATAAAGAGGGACAGAGACATACCTACTTAATAAAGTGTTGATTTTAATACCCAACTTCTAATATACACACATTTAAAGTAAATTCATTAAATGCTAATAATGGAGGAAATTGAACTCTAAAATACGGCACCTCACACTATAAGTTTCATTTATTATTGTTTTCTCTTAATGGTTAAGATATATATATTAGTCAATTAAAAAATATGTTTTTCTGGGTGAGTTATTTATAGAAACTCATTTTTTATACATATTAGTAGTATACAAAACTAAGTTTTTTTTTTTTTTTCTAATTTGGTTAAAATTCATTCATGTGTCTAATTTTAGTCCATATAATTTTAAAATTAGTCATGAAAATATATTTATAAAAAAAAAGTTGAAAATATATTAGAAAATAGAAAACAAATATATTATGAAATTGTGTGTATATATATATGTATGTATGTATGTATGTATGTATGTATATTTGCATTTAATATATTTCACTTTCTGCTCATCAAATTCAAGAGGGTTTATTTCACACATTAAGGATAGGTGGAGACCAGAAACACTATGATGTATCTTCTTGTCACAATATTTTATGTAAAATTTATGATTAAAAATAGTCTTTTGAATCTGAGACACAAATAAGTGAAATTAGTGAACGAAACAAATCCTTAATTTCAGAGAAAATATGGCATAATAATTGGATGAACTTTTATTGCTTTGACACAGGTTGATGTCAATGGCGGCTTCCCTGCTTGGGAAGCCATAAAATACCGGCCGGATATTGAGGAGAATCGGTCCAAATCCTCCGATGAGAGGCCTCTCCAGACGGGAATCATAGAACCCAACAACGAAACAGAATCATCCTTTCAAAAATCCCTCTTACAGAAGGGTCTCAGAGTCACTGGAGACGACAGCGACACCCTTTCCATCCAATGTGATGTGGTGGTTGTTGGGTCCGGTTGCGGCGGAGGCGTTGCGGCGGCGGTTCTCGCAAGTTCCGGCCAGAAAGTGGTGGTTCTCGAAAAGGGGAATTATTTTACTGCATCTGATTATTCATCTCTTGAAGGCCCTTCCCTTAATCAACTGTACGAAGCCGGTGGGATTTTCACAAGTGATGATGGGAAAATGTTGATTCTGGCAGGTTCAACCGTCGGCGGTGGCTCCGCCGTTAATTGGTCGGCGAGCATCAGAACCCCAAATCACGTTTTGCAGGAATGGGCTGAGAGCCACAAGATTCCGTTCTTTGGAACCTCTGAATATCAAATCGCAATGGATGCTGTGTGCAAGAGACTTGGGGTTTCAGAAGACTGTGATGAAGAGGGGTTTCAGAATCAAGTTCTTCGAAAAGGGTGTGAGAATTTGGGGCTTACAGTTGAAAAAGTACCAAGAAATTCCCCAAGCGGTCATTATTGTGGATCCTGCGGCTATGGTTGTAGAAGGGGGGAGAAACAAGGGACTGACTCTACTTGGCTCGTTGATGCTGTGAATCATGGGGCAGTGATCATAACAAATTGTAAGGCTGAGAGATTCATATTGGAAAGGAACAAAACTGGAGGTGTGAGGAAGAGCAAGTGCTTGGGTGTGCTTGCAAATGTTTGGAGTGACAACTTTACCAAGAAACTGCAAATCAAAGCCAAAGCTACCATCTCAGCTTGTGGGGCTCTTTTGACACCGCCATTGATGATATCAAGTGGGCTGAGGAACAAACACATTGGCAGAAATCTTCATCTCCACCCTGTTTTGATGGCTTGGGGATACTTTCCAGACTCAACTTCAGAGTTCAAGGGAAAATCCTATGAGGGGGGAATAATCACATCACTCCACAAGGTTATCTCTGAAGGATCCGAATCAAATTCTAAAACCATCATAGAGACTCCTTTATTAGGACCAGGATCATTCTCCGTGTTGTTTCCTTGGATATCTGGACTTGACACTAAGCAAAGAATGCTCAAGTATTCGAGAATTTCCCATTTGATAACAATCGTCCGGGATTCGGGATCCGGGGCAGTAAGATCGGAGGGAAGGGTGAGCTTCAATTTGAGTGAAGAAGACAGGGAAAACCTCAAGAGGGGGTTAAGACAGTCGTTGAGGAGTTATGGTAGCTGA

mRNA sequence

AGATCCCTCGTCTCGGAGCAGAGGCATATTCATATTCAATCAATCTACAGAGCGACGATGCGGAGATCGGAGTGCCATCCCCTGTTGAGAGGAACAAGGAGCGGCAAGTTCACGCATGGCTTCTCCGTGGCGGAGATGGAGTCTTTAGCCACCATCTGCGACACTATATTGCCTCCTCTGCCGCTCGGTTCATCAAACAACAACGGCAGCGATGAAATCGATACTGCCGATGCGGCCGCAGTCGAGGCGTTTTACGCGGCGTCCGGTTCGCGATCTCCGATTCCGGATGAGGTTGCAGAGACAGTCGCGAAAAGAGGATTGACTGAACTCGTGATACTGGTGCGGATGGTGCTGTGGTTGCTAGGTACGAGGCTAGGGACGTTGCTGCTCTGTGGATCGCTCTGTTTTTGCAGGACATGGCCTTATATCTGCAATTTCTCGAGTATGCCATTGAAGAACAGAGAGGAAGCTTTGAGGAGATGGTATAAGAATAAGGTTTTCACGCCCATTAGAGCTGCTCTTGCTTCCATCAGAGTTCTTGTCCTCTACACCTTCTTTTCTCGGGTTGATGTCAATGGCGGCTTCCCTGCTTGGGAAGCCATAAAATACCGGCCGGATATTGAGGAGAATCGGTCCAAATCCTCCGATGAGAGGCCTCTCCAGACGGGAATCATAGAACCCAACAACGAAACAGAATCATCCTTTCAAAAATCCCTCTTACAGAAGGGTCTCAGAGTCACTGGAGACGACAGCGACACCCTTTCCATCCAATGTGATGTGGTGGTTGTTGGGTCCGGTTGCGGCGGAGGCGTTGCGGCGGCGGTTCTCGCAAGTTCCGGCCAGAAAGTGGTGGTTCTCGAAAAGGGGAATTATTTTACTGCATCTGATTATTCATCTCTTGAAGGCCCTTCCCTTAATCAACTGTACGAAGCCGGTGGGATTTTCACAAGTGATGATGGGAAAATGTTGATTCTGGCAGGTTCAACCGTCGGCGGTGGCTCCGCCGTTAATTGGTCGGCGAGCATCAGAACCCCAAATCACGTTTTGCAGGAATGGGCTGAGAGCCACAAGATTCCGTTCTTTGGAACCTCTGAATATCAAATCGCAATGGATGCTGTGTGCAAGAGACTTGGGGTTTCAGAAGACTGTGATGAAGAGGGGTTTCAGAATCAAGTTCTTCGAAAAGGGTGTGAGAATTTGGGGCTTACAGTTGAAAAAGTACCAAGAAATTCCCCAAGCGGTCATTATTGTGGATCCTGCGGCTATGGTTGTAGAAGGGGGGAGAAACAAGGGACTGACTCTACTTGGCTCGTTGATGCTGTGAATCATGGGGCAGTGATCATAACAAATTGTAAGGCTGAGAGATTCATATTGGAAAGGAACAAAACTGGAGGTGTGAGGAAGAGCAAGTGCTTGGGTGTGCTTGCAAATGTTTGGAGTGACAACTTTACCAAGAAACTGCAAATCAAAGCCAAAGCTACCATCTCAGCTTGTGGGGCTCTTTTGACACCGCCATTGATGATATCAAGTGGGCTGAGGAACAAACACATTGGCAGAAATCTTCATCTCCACCCTGTTTTGATGGCTTGGGGATACTTTCCAGACTCAACTTCAGAGTTCAAGGGAAAATCCTATGAGGGGGGAATAATCACATCACTCCACAAGGTTATCTCTGAAGGATCCGAATCAAATTCTAAAACCATCATAGAGACTCCTTTATTAGGACCAGGATCATTCTCCGTGTTGTTTCCTTGGATATCTGGACTTGACACTAAGCAAAGAATGCTCAAGTATTCGAGAATTTCCCATTTGATAACAATCGTCCGGGATTCGGGATCCGGGGCAGTAAGATCGGAGGGAAGGGTGAGCTTCAATTTGAGTGAAGAAGACAGGGAAAACCTCAAGAGGGGGTTAAGACAGTCGTTGAGGAGTTATGGTAGCTGA

Coding sequence (CDS)

AGATCCCTCGTCTCGGAGCAGAGGCATATTCATATTCAATCAATCTACAGAGCGACGATGCGGAGATCGGAGTGCCATCCCCTGTTGAGAGGAACAAGGAGCGGCAAGTTCACGCATGGCTTCTCCGTGGCGGAGATGGAGTCTTTAGCCACCATCTGCGACACTATATTGCCTCCTCTGCCGCTCGGTTCATCAAACAACAACGGCAGCGATGAAATCGATACTGCCGATGCGGCCGCAGTCGAGGCGTTTTACGCGGCGTCCGGTTCGCGATCTCCGATTCCGGATGAGGTTGCAGAGACAGTCGCGAAAAGAGGATTGACTGAACTCGTGATACTGGTGCGGATGGTGCTGTGGTTGCTAGGTACGAGGCTAGGGACGTTGCTGCTCTGTGGATCGCTCTGTTTTTGCAGGACATGGCCTTATATCTGCAATTTCTCGAGTATGCCATTGAAGAACAGAGAGGAAGCTTTGAGGAGATGGTATAAGAATAAGGTTTTCACGCCCATTAGAGCTGCTCTTGCTTCCATCAGAGTTCTTGTCCTCTACACCTTCTTTTCTCGGGTTGATGTCAATGGCGGCTTCCCTGCTTGGGAAGCCATAAAATACCGGCCGGATATTGAGGAGAATCGGTCCAAATCCTCCGATGAGAGGCCTCTCCAGACGGGAATCATAGAACCCAACAACGAAACAGAATCATCCTTTCAAAAATCCCTCTTACAGAAGGGTCTCAGAGTCACTGGAGACGACAGCGACACCCTTTCCATCCAATGTGATGTGGTGGTTGTTGGGTCCGGTTGCGGCGGAGGCGTTGCGGCGGCGGTTCTCGCAAGTTCCGGCCAGAAAGTGGTGGTTCTCGAAAAGGGGAATTATTTTACTGCATCTGATTATTCATCTCTTGAAGGCCCTTCCCTTAATCAACTGTACGAAGCCGGTGGGATTTTCACAAGTGATGATGGGAAAATGTTGATTCTGGCAGGTTCAACCGTCGGCGGTGGCTCCGCCGTTAATTGGTCGGCGAGCATCAGAACCCCAAATCACGTTTTGCAGGAATGGGCTGAGAGCCACAAGATTCCGTTCTTTGGAACCTCTGAATATCAAATCGCAATGGATGCTGTGTGCAAGAGACTTGGGGTTTCAGAAGACTGTGATGAAGAGGGGTTTCAGAATCAAGTTCTTCGAAAAGGGTGTGAGAATTTGGGGCTTACAGTTGAAAAAGTACCAAGAAATTCCCCAAGCGGTCATTATTGTGGATCCTGCGGCTATGGTTGTAGAAGGGGGGAGAAACAAGGGACTGACTCTACTTGGCTCGTTGATGCTGTGAATCATGGGGCAGTGATCATAACAAATTGTAAGGCTGAGAGATTCATATTGGAAAGGAACAAAACTGGAGGTGTGAGGAAGAGCAAGTGCTTGGGTGTGCTTGCAAATGTTTGGAGTGACAACTTTACCAAGAAACTGCAAATCAAAGCCAAAGCTACCATCTCAGCTTGTGGGGCTCTTTTGACACCGCCATTGATGATATCAAGTGGGCTGAGGAACAAACACATTGGCAGAAATCTTCATCTCCACCCTGTTTTGATGGCTTGGGGATACTTTCCAGACTCAACTTCAGAGTTCAAGGGAAAATCCTATGAGGGGGGAATAATCACATCACTCCACAAGGTTATCTCTGAAGGATCCGAATCAAATTCTAAAACCATCATAGAGACTCCTTTATTAGGACCAGGATCATTCTCCGTGTTGTTTCCTTGGATATCTGGACTTGACACTAAGCAAAGAATGCTCAAGTATTCGAGAATTTCCCATTTGATAACAATCGTCCGGGATTCGGGATCCGGGGCAGTAAGATCGGAGGGAAGGGTGAGCTTCAATTTGAGTGAAGAAGACAGGGAAAACCTCAAGAGGGGGTTAAGACAGTCGTTGAGGAGTTATGGTAGCTGA

Protein sequence

RSLVSEQRHIHIQSIYRATMRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAAAVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRTWPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWEAIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCDVVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGVSEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVDAVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSEGRVSFNLSEEDRENLKRGLRQSLRSYGS
Homology
BLAST of Carg16550 vs. NCBI nr
Match: KAG7018802.1 (Long-chain-alcohol oxidase FAO1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 647/647 (100.00%), Postives = 647/647 (100.00%), Query Frame = 0

Query: 1   RSLVSEQRHIHIQSIYRATMRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPL 60
           RSLVSEQRHIHIQSIYRATMRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPL
Sbjct: 1   RSLVSEQRHIHIQSIYRATMRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPL 60

Query: 61  PLGSSNNNGSDEIDTADAAAVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWL 120
           PLGSSNNNGSDEIDTADAAAVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWL
Sbjct: 61  PLGSSNNNGSDEIDTADAAAVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWL 120

Query: 121 LGTRLGTLLLCGSLCFCRTWPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVL 180
           LGTRLGTLLLCGSLCFCRTWPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVL
Sbjct: 121 LGTRLGTLLLCGSLCFCRTWPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVL 180

Query: 181 VLYTFFSRVDVNGGFPAWEAIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLL 240
           VLYTFFSRVDVNGGFPAWEAIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLL
Sbjct: 181 VLYTFFSRVDVNGGFPAWEAIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLL 240

Query: 241 QKGLRVTGDDSDTLSIQCDVVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSL 300
           QKGLRVTGDDSDTLSIQCDVVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSL
Sbjct: 241 QKGLRVTGDDSDTLSIQCDVVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSL 300

Query: 301 EGPSLNQLYEAGGIFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPF 360
           EGPSLNQLYEAGGIFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPF
Sbjct: 301 EGPSLNQLYEAGGIFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPF 360

Query: 361 FGTSEYQIAMDAVCKRLGVSEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSC 420
           FGTSEYQIAMDAVCKRLGVSEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSC
Sbjct: 361 FGTSEYQIAMDAVCKRLGVSEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSC 420

Query: 421 GYGCRRGEKQGTDSTWLVDAVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWS 480
           GYGCRRGEKQGTDSTWLVDAVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWS
Sbjct: 421 GYGCRRGEKQGTDSTWLVDAVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWS 480

Query: 481 DNFTKKLQIKAKATISACGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEF 540
           DNFTKKLQIKAKATISACGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEF
Sbjct: 481 DNFTKKLQIKAKATISACGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEF 540

Query: 541 KGKSYEGGIITSLHKVISEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSR 600
           KGKSYEGGIITSLHKVISEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSR
Sbjct: 541 KGKSYEGGIITSLHKVISEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSR 600

Query: 601 ISHLITIVRDSGSGAVRSEGRVSFNLSEEDRENLKRGLRQSLRSYGS 648
           ISHLITIVRDSGSGAVRSEGRVSFNLSEEDRENLKRGLRQSLRSYGS
Sbjct: 601 ISHLITIVRDSGSGAVRSEGRVSFNLSEEDRENLKRGLRQSLRSYGS 647

BLAST of Carg16550 vs. NCBI nr
Match: KAG6582399.1 (Long-chain-alcohol oxidase FAO1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 621/624 (99.52%), Postives = 622/624 (99.68%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           M+RSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA
Sbjct: 1   MQRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 60

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCR 
Sbjct: 61  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 120

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE
Sbjct: 121 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           AIKYRPDIEENRSKSSDERPLQ GIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 240

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 480

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE
Sbjct: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 540

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
           GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 600

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVSFNLSEEDRENLKRGLRQSLR
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLR 624

BLAST of Carg16550 vs. NCBI nr
Match: XP_022924775.1 (long-chain-alcohol oxidase FAO1-like [Cucurbita moschata])

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 613/624 (98.24%), Postives = 616/624 (98.72%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRR ECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA
Sbjct: 1   MRRLECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 60

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           A+EAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCR 
Sbjct: 61  AIEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 120

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WPYICNFSSMPL+NREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE
Sbjct: 121 WPYICNFSSMPLENREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           AIKYRPDIEENRSKSSDERPLQ GIIEPNNETESSFQKSLLQKGLRVTGDD DTLSIQCD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGDDGDTLSIQCD 240

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDCDEEGFQNQVLRKGCENLGL VEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           AVNHGAVIITNCKAERFILERNKTG VRKSKCLGVLANVWSDNFTKKLQIKAKATISACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 480

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPP+MISSGLRNKHIGRNLHLHPVLMAWGYFPDS SEFKGKSYEGGIITSLHKV SE
Sbjct: 481 ALLTPPVMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVTSE 540

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
           GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 600

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVSFNLSEEDRENLKRGLRQSLR
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLR 624

BLAST of Carg16550 vs. NCBI nr
Match: XP_023527426.1 (long-chain-alcohol oxidase FAO1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 608/624 (97.44%), Postives = 611/624 (97.92%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNG+DEIDT DAA
Sbjct: 1   MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGNDEIDTTDAA 60

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           A+EAFYA SGSRSPIPDEVAETVAKRGLTELVILVRMVLWLL TRLGTLLLCGSLCFCR 
Sbjct: 61  AIEAFYAESGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLATRLGTLLLCGSLCFCRK 120

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WPYICNFSSMPL+NREEALRRW KNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE
Sbjct: 121 WPYICNFSSMPLENREEALRRWCKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           AIKYRPDIEENRSKSSDERPLQ GIIEPNNETESSFQKSLLQKGLRVTG D DTLSIQCD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGHDGDTLSIQCD 240

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDCDEEGFQNQVLRKGCENLGL VEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           AVNHGAVIITNCKAERFILERNKTG VRKSKCLGV ANVWSDNFTKKLQIKAKATISACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVRANVWSDNFTKKLQIKAKATISACG 480

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDS SEFKGKSYEGGIITSLHKVISE
Sbjct: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVISE 540

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
           GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRD GSGAVRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDLGSGAVRSE 600

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVSFNLSEEDRENLKRGLRQSLR
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLR 624

BLAST of Carg16550 vs. NCBI nr
Match: XP_022979358.1 (long-chain-alcohol oxidase FAO1-like [Cucurbita maxima])

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 602/624 (96.47%), Postives = 607/624 (97.28%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTI PPLP GSSNNNGSDEIDTADAA
Sbjct: 2   MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTIFPPLPFGSSNNNGSDEIDTADAA 61

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCR 
Sbjct: 62  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 121

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WPYICNFSSMPL+NREEALRRW+KNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE
Sbjct: 122 WPYICNFSSMPLENREEALRRWFKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 181

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           A+KYRP IEEN SKSSDERPLQ GIIEPN ETESSF+KS LQKGLRVTGDD  TLSIQCD
Sbjct: 182 AVKYRPGIEENPSKSSDERPLQKGIIEPNKETESSFKKSFLQKGLRVTGDDGYTLSIQCD 241

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTS+D
Sbjct: 242 VVVVGSGCGGGVAAAFLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSND 301

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQ AMDAVCKRLGV
Sbjct: 302 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQSAMDAVCKRLGV 361

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDCDEEGFQNQVLRKGCENLGL VEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD
Sbjct: 362 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 421

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           AVNHGAVIITNCKAERFILERNKTG VRKSKCLGVLANVWSDNFTKKLQIKAKATI ACG
Sbjct: 422 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATILACG 481

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDS SEFKGKSYEGGIITSLHKVISE
Sbjct: 482 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVISE 541

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
           GSESNSKTIIETP LGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE
Sbjct: 542 GSESNSKTIIETPSLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 601

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVSFNLSEEDRENLKRGLRQSLR
Sbjct: 602 GRVSFNLSEEDRENLKRGLRQSLR 625

BLAST of Carg16550 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 4.6e-224
Identity = 394/626 (62.94%), Postives = 471/626 (75.24%), Query Frame = 0

Query: 22  RSECHPLLRGTR-SGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAAA 81
           R ECHPLLRG R   KF  GFS +EMESLA+IC+ ILPPLP+ +      D++   D   
Sbjct: 2   RKECHPLLRGGRKDNKFRQGFSSSEMESLASICEVILPPLPMDALKIKKHDDVSIED--- 61

Query: 82  VEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRTW 141
           VE F+  S S  PIP EVAE ++KR L E VIL+R+VLWLL TRLGT LLCG LCF   W
Sbjct: 62  VEFFWNTSASHYPIPHEVAEILSKRSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKW 121

Query: 142 PYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWEA 201
           P+I NFSSM LK RE  ++ W KN+  TPIR A A ++VL L+ +F+ VD NG  PAW+A
Sbjct: 122 PFINNFSSMSLKKRERVMQWWLKNRFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKA 181

Query: 202 IKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDD-SDTLSIQCD 261
           I Y    +E    SS+ R L+ GI+E  +ET S+ Q+SL +KGL VT D  ++ L ++CD
Sbjct: 182 IGYEVSADEKLPNSSNGRLLEKGIVETMHETNSTLQQSLTEKGLNVTLDSRNNILKVKCD 241

Query: 262 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 321
            +VVGSGCGGGVAA+VL+ +G KVVVLEKGNYF   DYSSLEGPS++QLYE GGI  S D
Sbjct: 242 AIVVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLYETGGILASVD 301

Query: 322 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 381
            ++L+LAGSTVGGGSAVNWSA I+TP+ VL+EW+E+H +  F TSEY  AM+ VC+R+GV
Sbjct: 302 SRILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSAMETVCERIGV 361

Query: 382 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 441
           +E C  EGFQNQVLRKGC+NLGL V+ VPRNS   H+CGSCGYGC +GEKQGT  TWLVD
Sbjct: 362 TESCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEKQGTQDTWLVD 421

Query: 442 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 501
           AV +GAVIIT CKAERF+LE N+ G  RK KCLGVLA   S   T KLQI+AK TISA G
Sbjct: 422 AVENGAVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQIEAKVTISAGG 481

Query: 502 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 561
           ALLTPPLMISSGL+NK+IG+NLHLHPVLM WGYFP+STSE KGK +EGGIITS+HKV S 
Sbjct: 482 ALLTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTSELKGKIFEGGIITSVHKVPSR 541

Query: 562 GSESNSKT--IIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVR 621
            S S S T  IIETP LGPGSF+ L PW SGLD K+RML Y R +HLITI+RD   G V 
Sbjct: 542 DSNSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTAHLITIIRDKACGQVT 601

Query: 622 SEGRVSFNLSEEDRENLKRGLRQSLR 644
           +EGRVS+ LS  D+EN+K GL+Q+LR
Sbjct: 602 TEGRVSYKLSAFDKENMKCGLQQALR 624

BLAST of Carg16550 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.9e-202
Identity = 362/617 (58.67%), Postives = 456/617 (73.91%), Query Frame = 0

Query: 34  SGKF-THGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAAAVEAFYAASGSRS 93
           +GKF     +VAEMESLA+ C+ +LP +       +G  + +  +  A+ +FY+ SGS++
Sbjct: 7   AGKFGLPDITVAEMESLASFCEAVLPSVQPPPEELSGEGD-NHRNKEALRSFYSTSGSKT 66

Query: 94  PIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRTWPYICNFSSMPLK 153
           P+  +  E V KRG  E  I  R++L+LL TRLGTLL+CG+ C    WP++  FS + L+
Sbjct: 67  PVLRQSIELVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLE 126

Query: 154 NREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWEAIKYRPDIEENR- 213
            RE  L++ +KN + TPIRAA   I+V  L+ FFSRV+ NG  PAWEAI YR + +EN+ 
Sbjct: 127 KRERVLQKQFKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKP 186

Query: 214 SKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGD-DSDTLSIQCDVVVVGSGCGGG 273
           S++ +ERPL+ GI+E   ETE +  +SL  KGL    D + D + I+CDVVVVGSG GGG
Sbjct: 187 SETHNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDTEHDAIRIKCDVVVVGSGSGGG 246

Query: 274 VAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDDGKMLILAGSTV 333
           VAA+VLA SG KVVVLEKG+YFT S++   EGP L++LYE GGI  S DG  ++LAG+TV
Sbjct: 247 VAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATV 306

Query: 334 GGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGVSEDCDEEGFQN 393
           GGGSAVNWSA I+TP  VLQEW+E   IP FGT EY  AM+ V KR+GV+E C+ E FQN
Sbjct: 307 GGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQN 366

Query: 394 QVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVDAVNHGAVIITN 453
           Q+LRKGCENLG  VE VPRNS   HYCGSCGYGCR+G+K+G+D TWLVDAV HGAVI+T 
Sbjct: 367 QILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTG 426

Query: 454 CKAERFILERNKTG-GVRKSKCLGVLANVWSDNFTKKLQIKAKATISACGALLTPPLMIS 513
           CKAERFILE+N +  G ++ KCLGV+A   + N  K L+I+AK T+SA GALLTPPLMIS
Sbjct: 427 CKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMIS 486

Query: 514 SGLRNKHIGRNLHLHPVLMAWGYFPDSTS---EFKGKSYEGGIITSLHKVISEGSESNSK 573
           SGLRN++IG+NLHLHPVLMAWGYFPD  S    FKG SYEGGIITS+ KV+SE SE   +
Sbjct: 487 SGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSE--VR 546

Query: 574 TIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSEGRVSFNL 633
            IIETP LGPGSFSVL PW SGLD K+RM +YSR + LITIVRD GSG V++EGR+++ +
Sbjct: 547 AIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTV 606

Query: 634 SEEDRENLKRGLRQSLR 644
            + DR+NLK GLR+SLR
Sbjct: 607 DKTDRDNLKAGLRESLR 620

BLAST of Carg16550 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 6.0e-200
Identity = 350/628 (55.73%), Postives = 454/628 (72.29%), Query Frame = 0

Query: 24  ECHPLLRGTRSGK--FTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAAAV 83
           E HPLL+G R  +  ++HG S ++M  +A IC+ + P  PL S NN  S +       A+
Sbjct: 8   ETHPLLKGGRRKEKGYSHGLSSSQMHVIAAICEALFPSQPLDSQNNQSSVD------KAL 67

Query: 84  EAFYAASGSRSPIPDEVAETVAK--RGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 143
            AFY ASGS++P+PDE AE + K  R   E + LV  VL +L  RLGTLLLCG+LC    
Sbjct: 68  SAFYTASGSQAPLPDEAAELLFKFNRSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWR 127

Query: 144 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 203
           WP+I  FS +PL+ REE L+RW + K + P+R      +++  Y  FSR DVNG  P W+
Sbjct: 128 WPFIHKFSEIPLEKREEILKRWSREKCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWK 187

Query: 204 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGD-DSDTLSIQC 263
           AI Y+ D  E  ++   +RPLQ G+IE   ET+S+  +SL +KGL VT D + +   I+C
Sbjct: 188 AIGYQVDTREKLTQK--KRPLQEGLIETMYETDSTLIQSLTEKGLEVTEDLEQNMYKIKC 247

Query: 264 DVVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSD 323
           D V+VGSGCGGGVAAAVLA+SG KV++LEKG YF + DYSSLEGPS+N+LYE+GGI  S 
Sbjct: 248 DAVIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESGGILPSL 307

Query: 324 DGKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLG 383
           DGKM+ILAGST+GG SA+NWSA IRTP+ VL+EW+E HKIP F + +YQ AMD VC+R+G
Sbjct: 308 DGKMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDTVCRRIG 367

Query: 384 VSEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLV 443
           V+E+C++E FQNQ+LR+GC  +G  VE V  NS + HYCGSC YGCR G+K+GT+STWLV
Sbjct: 368 VTENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGTESTWLV 427

Query: 444 DAVNHGAVIITNCKAER--FILERNKTGGVRKSKCLGVLAN-VWSDNFTKKLQIKAKATI 503
           DAV +GAVI+T CKAE+  F L+    G  RK+ C GV+A+  W    TKKLQI++K TI
Sbjct: 428 DAVGNGAVILTGCKAEKLNFTLKDGDNGTKRKT-CSGVIASATWRSKVTKKLQIESKVTI 487

Query: 504 SACGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHK 563
           SACG+L TPPLMISSGL+N +IG+NLHLHP   AWGYFP+  + F G +YEGGIITS+HK
Sbjct: 488 SACGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNFSGNNYEGGIITSIHK 547

Query: 564 VISEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGA 623
           V  E S S  + IIE P LGPGSFS L PW+SGLD K+RM+KY+R ++L  +VRD GSG 
Sbjct: 548 VFEEDSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYARTANLFALVRDHGSGE 607

Query: 624 VRSEGRVSFNLSEEDRENLKRGLRQSLR 644
           V++EGR+S+ L + DRE+L+ GLR++LR
Sbjct: 608 VKAEGRISYKLDKIDRESLQTGLRKALR 626

BLAST of Carg16550 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 660.6 bits (1703), Expect = 1.8e-188
Identity = 343/632 (54.27%), Postives = 436/632 (68.99%), Query Frame = 0

Query: 21  RRSECHPLLRGTRSGK-FTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 80
           RR+  HPLLR  + G+ + HGFS ++++SLA IC T LPP               T++  
Sbjct: 6   RRNRGHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPP-------------ETTSEQQ 65

Query: 81  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 140
           AV +F+ AS ++ P  DEVAE + K G +E V ++R++L +L  R GTLLLCGSLC  ++
Sbjct: 66  AVNSFHVASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKS 125

Query: 141 WPYICNFSSMPLKNREEALRRWYKNKVF-TPIRAALASIRVLVLYTFFSRVDVNGGFPAW 200
           WP++  FS +PL  RE  LR W +   F  P R      +   L+ FFS+ D N   PA 
Sbjct: 126 WPFVLKFSQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPAL 185

Query: 201 EAIKYRPDIEE---NRSKSSDE--RPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDT 260
           EAI Y  D  E   N+   +DE  RPL+ GIIE  +E++ +  +SL +KG+ V  DD D 
Sbjct: 186 EAIGYCIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVARDDGDN 245

Query: 261 L-SIQCDVVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAG 320
           +  I+CD VVVGSG GGGVAAA LA +G KV+VLEKGNYFTA DYS LE PS+ +LYE G
Sbjct: 246 VYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKG 305

Query: 321 GIFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDA 380
           G+ T+ DGK ++LAGS VGGG+AVNWSASIRTP+HVLQEW+E  KI FFG+ EYQ AMD 
Sbjct: 306 GLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDE 365

Query: 381 VCKRLGVSEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGT 440
           V  R+GV+E C + GFQNQVLRKGCE LGL VE VPRNSP  HYCG CGYGCR G K GT
Sbjct: 366 VTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGT 425

Query: 441 DSTWLVDAVNHGAVIITNCKAERFIL-ERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKA 500
           D TWLVDAV +GAVI+T  KAERF+L +   +   RK +C+GV A+       KK  I+A
Sbjct: 426 DQTWLVDAVENGAVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEA 485

Query: 501 KATISACGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIIT 560
           + T+S+ G+LLTPPLM+SSGL+N +IGRNL LHPVLM WGYFP+  SEF GK YEGGIIT
Sbjct: 486 RVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFPEKDSEFSGKMYEGGIIT 545

Query: 561 SLHKVISEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDS 620
           S+H +    +ES  K I+E PL+GP S++ L PW+SG D K+RM+KY R +HL  +VRD 
Sbjct: 546 SVHHM--NDTESGCKAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 605

Query: 621 GSGAVRSEGRVSFNLSEEDRENLKRGLRQSLR 644
           GSG V  E  V++  +++DRENL+ GLRQ+LR
Sbjct: 606 GSGEVMMENEVTYRTTKKDRENLRAGLRQALR 622

BLAST of Carg16550 vs. ExPASy Swiss-Prot
Match: Q9ZWB9 (Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=2)

HSP 1 Score: 599.0 bits (1543), Expect = 6.5e-170
Identity = 311/626 (49.68%), Postives = 428/626 (68.37%), Query Frame = 0

Query: 27  PLLR-GTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAAAVEAFY 86
           PLLR   +   F+HGFS +++++L++ICD I+PP+PL S N     ++   D  A+ +F+
Sbjct: 13  PLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLRND--ALLSFF 72

Query: 87  AASGSRSPI-PDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT-WPYI 146
            +S S S + PDEVAE +A + +   V++VR+VL +L  RLGTLLLCG +C  +  WP++
Sbjct: 73  KSSSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFL 132

Query: 147 CNFSSMPLKNREEALRRWYKNKVFTPI-RAALASIRVLVLYTFFSRVDVNGGFPAWEAIK 206
             FS M L+ RE+ L+RW   + + P+ R     I+ + L+ +F+  + N   P W+AI 
Sbjct: 133 LKFSEMSLEKREKVLQRW-NTQWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAIN 192

Query: 207 YRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGD-DSDTLSIQCDVV 266
           Y  +I EN      ERPL  GIIE   E E + ++ ++ KGL+VT D + DT  I+CD V
Sbjct: 193 YSVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRERDTYKIECDAV 252

Query: 267 VVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDDGK 326
           VVGSGCGGGVAAA+LA SG +VVV+EKGNYF   DYS+LEGPS+ +L+E+  +  + DG+
Sbjct: 253 VVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFELFESNSLMMTHDGR 312

Query: 327 MLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGVSE 386
              +AGSTVGGGS VNW+AS++TP+ +++EW+    I  + + +Y+ AM  VCKRLGV+E
Sbjct: 313 FRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYKAAMGIVCKRLGVTE 372

Query: 387 DCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVDAV 446
               EGFQNQ+LRKGCE LGL V  VPRNS   HYCGSC YGC  GEK+GTDSTWLVDAV
Sbjct: 373 KIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAV 432

Query: 447 NHGAVIITNCKAERFILERN----KTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISA 506
           N+ AVI+T CKAE+ IL  N    +    R+ +CLGV A++ S    KKLQI AK TI A
Sbjct: 433 NNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAASL-SHQTRKKLQINAKVTIVA 492

Query: 507 CGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVI 566
           CG+L TP L+ SSGL+N +I R LH+HP++MAWGYFP+  SE +G ++EG I+TSLH V 
Sbjct: 493 CGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNSELEGAAHEGEIVTSLHYVH 552

Query: 567 SEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVR 626
              S + + T +ETP +GPG+F+ L PW+SG D K+RM KY+R +H+  +VRD G G V+
Sbjct: 553 PMDSTTPNIT-LETPAIGPGTFAALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVK 612

Query: 627 SEGRVSFNLSEEDRENLKRGLRQSLR 644
            +  V + L++ D ENL  GL+Q+LR
Sbjct: 613 GD-IVKYRLTKADEENLTIGLKQALR 632

BLAST of Carg16550 vs. ExPASy TrEMBL
Match: A0A6J1EG06 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111432173 PE=3 SV=1)

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 613/624 (98.24%), Postives = 616/624 (98.72%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRR ECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA
Sbjct: 1   MRRLECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 60

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           A+EAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCR 
Sbjct: 61  AIEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 120

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WPYICNFSSMPL+NREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE
Sbjct: 121 WPYICNFSSMPLENREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 180

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           AIKYRPDIEENRSKSSDERPLQ GIIEPNNETESSFQKSLLQKGLRVTGDD DTLSIQCD
Sbjct: 181 AIKYRPDIEENRSKSSDERPLQKGIIEPNNETESSFQKSLLQKGLRVTGDDGDTLSIQCD 240

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD
Sbjct: 241 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 300

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 360

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDCDEEGFQNQVLRKGCENLGL VEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD
Sbjct: 361 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 420

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           AVNHGAVIITNCKAERFILERNKTG VRKSKCLGVLANVWSDNFTKKLQIKAKATISACG
Sbjct: 421 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 480

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPP+MISSGLRNKHIGRNLHLHPVLMAWGYFPDS SEFKGKSYEGGIITSLHKV SE
Sbjct: 481 ALLTPPVMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVTSE 540

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
           GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE
Sbjct: 541 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 600

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVSFNLSEEDRENLKRGLRQSLR
Sbjct: 601 GRVSFNLSEEDRENLKRGLRQSLR 624

BLAST of Carg16550 vs. ExPASy TrEMBL
Match: A0A6J1IVY9 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479108 PE=3 SV=1)

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 602/624 (96.47%), Postives = 607/624 (97.28%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTI PPLP GSSNNNGSDEIDTADAA
Sbjct: 2   MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTIFPPLPFGSSNNNGSDEIDTADAA 61

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCR 
Sbjct: 62  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRK 121

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WPYICNFSSMPL+NREEALRRW+KNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE
Sbjct: 122 WPYICNFSSMPLENREEALRRWFKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 181

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           A+KYRP IEEN SKSSDERPLQ GIIEPN ETESSF+KS LQKGLRVTGDD  TLSIQCD
Sbjct: 182 AVKYRPGIEENPSKSSDERPLQKGIIEPNKETESSFKKSFLQKGLRVTGDDGYTLSIQCD 241

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAA LASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTS+D
Sbjct: 242 VVVVGSGCGGGVAAAFLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSND 301

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQ AMDAVCKRLGV
Sbjct: 302 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQSAMDAVCKRLGV 361

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDCDEEGFQNQVLRKGCENLGL VEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD
Sbjct: 362 SEDCDEEGFQNQVLRKGCENLGLKVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 421

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           AVNHGAVIITNCKAERFILERNKTG VRKSKCLGVLANVWSDNFTKKLQIKAKATI ACG
Sbjct: 422 AVNHGAVIITNCKAERFILERNKTGSVRKSKCLGVLANVWSDNFTKKLQIKAKATILACG 481

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDS SEFKGKSYEGGIITSLHKVISE
Sbjct: 482 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSNSEFKGKSYEGGIITSLHKVISE 541

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
           GSESNSKTIIETP LGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE
Sbjct: 542 GSESNSKTIIETPSLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 601

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVSFNLSEEDRENLKRGLRQSLR
Sbjct: 602 GRVSFNLSEEDRENLKRGLRQSLR 625

BLAST of Carg16550 vs. ExPASy TrEMBL
Match: A0A5A7U0C1 (Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001810 PE=3 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 2.2e-306
Identity = 518/624 (83.01%), Postives = 564/624 (90.38%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRRSECHPLLRGTR+GKFTHGFS AEMESL +ICDT+LPPLP  S          T   A
Sbjct: 1   MRRSECHPLLRGTRTGKFTHGFSTAEMESLTSICDTVLPPLPFNS---------PTTSTA 60

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           A  AFYA SG++SPIPDEVAE   KRGL E+V+LVR+VLW+L TRLGTLLLCGSLCFC  
Sbjct: 61  AERAFYAVSGAQSPIPDEVAEMARKRGLIEIVLLVRVVLWILATRLGTLLLCGSLCFCEK 120

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WP++  FS MPLKNREEALRRW+KNKVFTPIRAALA I+++ LY FFSRVDVNG  P+WE
Sbjct: 121 WPFVWKFSEMPLKNREEALRRWFKNKVFTPIRAALALIKLVSLYIFFSRVDVNGDNPSWE 180

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           AIKY P+I+E+ SKSSD RPLQ GIIEPNNETESSF+KSLLQKG+R+ GDD  TLS++CD
Sbjct: 181 AIKYVPEIDEHPSKSSDVRPLQKGIIEPNNETESSFKKSLLQKGIRIAGDDGHTLSVECD 240

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAA+LASSGQKV+VLEKGNYFTASDYSSLEGPSL QLYEAGGIF +DD
Sbjct: 241 VVVVGSGCGGGVAAAILASSGQKVMVLEKGNYFTASDYSSLEGPSLEQLYEAGGIFATDD 300

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTP+HVLQEWAESHKIPFFGTSEYQ+AMDAVCKR+GV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPDHVLQEWAESHKIPFFGTSEYQVAMDAVCKRIGV 360

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDC+EEGFQNQVLRKGCENLGL VEKV RNSP+GHYCGSCGYGCR+GEKQGTDSTWLVD
Sbjct: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVARNSPTGHYCGSCGYGCRKGEKQGTDSTWLVD 420

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           +VNHGAVIITNCKAERFIL RNK G +RKSKCLGV+ANVWSDN TKKL IKAKATISACG
Sbjct: 421 SVNHGAVIITNCKAERFILARNKNGSMRKSKCLGVIANVWSDNITKKLHIKAKATISACG 480

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPPLMISSGLRNKHIGRNLHLHPVLM WGYFP+S S FKGKSYEGGIITS+HKV+SE
Sbjct: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESDSGFKGKSYEGGIITSVHKVVSE 540

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
            S+SN KTIIETPLLGPGS +VL PW+SGLDTKQRMLKYSR +H ITI+RDSGSGAVRSE
Sbjct: 541 ESKSNPKTIIETPLLGPGSCAVLVPWVSGLDTKQRMLKYSRTAHFITILRDSGSGAVRSE 600

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVS+NLSEEDRENLK+GLRQSLR
Sbjct: 601 GRVSYNLSEEDRENLKKGLRQSLR 615

BLAST of Carg16550 vs. ExPASy TrEMBL
Match: A0A1S3AW60 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103483573 PE=3 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 6.5e-306
Identity = 518/624 (83.01%), Postives = 563/624 (90.22%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRRSECHPLLRGTR+GKFTH FS AEMESL +ICDT+LPPLP  S          T   A
Sbjct: 1   MRRSECHPLLRGTRTGKFTHEFSTAEMESLTSICDTVLPPLPFNS---------PTTSTA 60

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           A  AFYA SG++SPIPDEVAE   KRGL E+V+LVR+VLW+L TRLGTLLLCGSLCFC  
Sbjct: 61  AERAFYAVSGAQSPIPDEVAEMARKRGLVEIVLLVRVVLWILATRLGTLLLCGSLCFCEK 120

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WP++  FS MPLKNREEALRRW+KNKVFTPIRAALA I+++ LY FFSRVDVNG  P+WE
Sbjct: 121 WPFVWKFSEMPLKNREEALRRWFKNKVFTPIRAALALIKLVSLYIFFSRVDVNGDNPSWE 180

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           AIKY P+I+E+ SKSSD RPLQ GIIEPNNETESSF+KSLLQKG+R+ GDD  TLS++CD
Sbjct: 181 AIKYVPEIDEHPSKSSDVRPLQKGIIEPNNETESSFKKSLLQKGIRIAGDDGHTLSVECD 240

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVVVGSGCGGGVAAA+LASSGQKV+VLEKGNYFTASDYSSLEGPSL QLYEAGGIF +DD
Sbjct: 241 VVVVGSGCGGGVAAAILASSGQKVMVLEKGNYFTASDYSSLEGPSLEQLYEAGGIFATDD 300

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKMLILAGSTVGGGSAVNWSASIRTP+HVLQEWAESHKIPFFGTSEYQ+AMDAVCKR+GV
Sbjct: 301 GKMLILAGSTVGGGSAVNWSASIRTPDHVLQEWAESHKIPFFGTSEYQVAMDAVCKRIGV 360

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDC+EEGFQNQVLRKGCENLGL VEKV RNSP+GHYCGSCGYGCR+GEKQGTDSTWLVD
Sbjct: 361 SEDCEEEGFQNQVLRKGCENLGLKVEKVARNSPTGHYCGSCGYGCRKGEKQGTDSTWLVD 420

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           +VNHGAVIITNCKAERFIL RNK G +RKSKCLGV+ANVWSDN TKKL IKAKATISACG
Sbjct: 421 SVNHGAVIITNCKAERFILARNKNGSMRKSKCLGVIANVWSDNITKKLHIKAKATISACG 480

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPPLMISSGLRNKHIGRNLHLHPVLM WGYFP+S S FKGKSYEGGIITS+HKV+SE
Sbjct: 481 ALLTPPLMISSGLRNKHIGRNLHLHPVLMTWGYFPESDSGFKGKSYEGGIITSVHKVVSE 540

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
            S+SN KTIIETPLLGPGS +VL PW+SGLDTKQRMLKYSR +H ITIVRDSGSGAVRSE
Sbjct: 541 ESKSNPKTIIETPLLGPGSCAVLVPWVSGLDTKQRMLKYSRTAHFITIVRDSGSGAVRSE 600

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRVS+NLSEEDRENLK+GLRQSLR
Sbjct: 601 GRVSYNLSEEDRENLKKGLRQSLR 615

BLAST of Carg16550 vs. ExPASy TrEMBL
Match: A0A6J1IBI0 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111473170 PE=3 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 9.1e-300
Identity = 503/624 (80.61%), Postives = 561/624 (89.90%), Query Frame = 0

Query: 20  MRRSECHPLLRGTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 79
           MRRSECHPLLRGTRS KFTHGFS AEMES+  ICDT+LP LP  S      DE + +D A
Sbjct: 1   MRRSECHPLLRGTRSNKFTHGFSAAEMESITAICDTVLPSLPFDS-----QDETNASDIA 60

Query: 80  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 139
           +VEAFY+ASGSRSPIPDEVAE + K GL E+VIL+R+V+WLL TRLGTLLLCGSLCF   
Sbjct: 61  SVEAFYSASGSRSPIPDEVAEMITKIGLIEIVILIRIVMWLLATRLGTLLLCGSLCFREK 120

Query: 140 WPYICNFSSMPLKNREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWE 199
           WP++CNFS+M LKNREEAL+RW KNK+FT IR ALASIR+L LY FFSRVDVNG  PAWE
Sbjct: 121 WPFVCNFSAMALKNREEALKRWSKNKIFTFIRVALASIRILSLYIFFSRVDVNGDNPAWE 180

Query: 200 AIKYRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDTLSIQCD 259
           AIKYRP I+EN SKSSDERPLQ GIIEPNN+TESSFQ SLL+KGL V G+++ TLSI+CD
Sbjct: 181 AIKYRPAIDENPSKSSDERPLQKGIIEPNNKTESSFQNSLLEKGLTVIGEENHTLSIECD 240

Query: 260 VVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDD 319
           VVV+GSGCGGGVAAA+LASSGQKVVVLEKGNYFTASDYS+ EGPSL+QLYEAGG+ TS+D
Sbjct: 241 VVVIGSGCGGGVAAAILASSGQKVVVLEKGNYFTASDYSTFEGPSLDQLYEAGGVLTSND 300

Query: 320 GKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGV 379
           GKM +LAGSTVGGGSAVNWSASIRTPN VLQEW ES KIPFFG+SEYQ AMDAVCKR+GV
Sbjct: 301 GKMFVLAGSTVGGGSAVNWSASIRTPNAVLQEWVESDKIPFFGSSEYQNAMDAVCKRIGV 360

Query: 380 SEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVD 439
           SEDC++EGFQNQVLRKGCENLG  +EKVPRNSP+ HYCGSCGYGCR+GEKQGTD+TWLVD
Sbjct: 361 SEDCEQEGFQNQVLRKGCENLGFQIEKVPRNSPNTHYCGSCGYGCRKGEKQGTDTTWLVD 420

Query: 440 AVNHGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACG 499
           AVNHGAV+ITNCKAER ILERN+ G +RK+KCLGV+A VWSDN TKKL+I+AKATISACG
Sbjct: 421 AVNHGAVVITNCKAERLILERNRAGSMRKNKCLGVIAKVWSDNITKKLKIRAKATISACG 480

Query: 500 ALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVISE 559
           ALLTPPL+ISSGL+NKHIGRNLHLHPVLM WGYFPDS SEFKGKSYEGGIITS+HKV+SE
Sbjct: 481 ALLTPPLLISSGLKNKHIGRNLHLHPVLMTWGYFPDSNSEFKGKSYEGGIITSVHKVVSE 540

Query: 560 GSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 619
           G+ SN KTIIETPLLGPGSF++LFPWISGLD+K+RMLKYSR +H ITIVRDSGSG VRSE
Sbjct: 541 GTNSNPKTIIETPLLGPGSFAILFPWISGLDSKKRMLKYSRTAHFITIVRDSGSGVVRSE 600

Query: 620 GRVSFNLSEEDRENLKRGLRQSLR 644
           GRV +NLSEED+ENLK GLRQSLR
Sbjct: 601 GRVRYNLSEEDKENLKIGLRQSLR 619

BLAST of Carg16550 vs. TAIR 10
Match: AT3G23410.1 (fatty alcohol oxidase 3 )

HSP 1 Score: 705.7 bits (1820), Expect = 3.5e-203
Identity = 362/617 (58.67%), Postives = 456/617 (73.91%), Query Frame = 0

Query: 34  SGKF-THGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAAAVEAFYAASGSRS 93
           +GKF     +VAEMESLA+ C+ +LP +       +G  + +  +  A+ +FY+ SGS++
Sbjct: 7   AGKFGLPDITVAEMESLASFCEAVLPSVQPPPEELSGEGD-NHRNKEALRSFYSTSGSKT 66

Query: 94  PIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRTWPYICNFSSMPLK 153
           P+  +  E V KRG  E  I  R++L+LL TRLGTLL+CG+ C    WP++  FS + L+
Sbjct: 67  PVLRQSIELVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLE 126

Query: 154 NREEALRRWYKNKVFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWEAIKYRPDIEENR- 213
            RE  L++ +KN + TPIRAA   I+V  L+ FFSRV+ NG  PAWEAI YR + +EN+ 
Sbjct: 127 KRERVLQKQFKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKP 186

Query: 214 SKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGD-DSDTLSIQCDVVVVGSGCGGG 273
           S++ +ERPL+ GI+E   ETE +  +SL  KGL    D + D + I+CDVVVVGSG GGG
Sbjct: 187 SETHNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDTEHDAIRIKCDVVVVGSGSGGG 246

Query: 274 VAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDDGKMLILAGSTV 333
           VAA+VLA SG KVVVLEKG+YFT S++   EGP L++LYE GGI  S DG  ++LAG+TV
Sbjct: 247 VAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATV 306

Query: 334 GGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGVSEDCDEEGFQN 393
           GGGSAVNWSA I+TP  VLQEW+E   IP FGT EY  AM+ V KR+GV+E C+ E FQN
Sbjct: 307 GGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQN 366

Query: 394 QVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVDAVNHGAVIITN 453
           Q+LRKGCENLG  VE VPRNS   HYCGSCGYGCR+G+K+G+D TWLVDAV HGAVI+T 
Sbjct: 367 QILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTG 426

Query: 454 CKAERFILERNKTG-GVRKSKCLGVLANVWSDNFTKKLQIKAKATISACGALLTPPLMIS 513
           CKAERFILE+N +  G ++ KCLGV+A   + N  K L+I+AK T+SA GALLTPPLMIS
Sbjct: 427 CKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMIS 486

Query: 514 SGLRNKHIGRNLHLHPVLMAWGYFPDSTS---EFKGKSYEGGIITSLHKVISEGSESNSK 573
           SGLRN++IG+NLHLHPVLMAWGYFPD  S    FKG SYEGGIITS+ KV+SE SE   +
Sbjct: 487 SGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSE--VR 546

Query: 574 TIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSEGRVSFNL 633
            IIETP LGPGSFSVL PW SGLD K+RM +YSR + LITIVRD GSG V++EGR+++ +
Sbjct: 547 AIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTV 606

Query: 634 SEEDRENLKRGLRQSLR 644
            + DR+NLK GLR+SLR
Sbjct: 607 DKTDRDNLKAGLRESLR 620

BLAST of Carg16550 vs. TAIR 10
Match: AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 660.6 bits (1703), Expect = 1.3e-189
Identity = 343/632 (54.27%), Postives = 436/632 (68.99%), Query Frame = 0

Query: 21  RRSECHPLLRGTRSGK-FTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAA 80
           RR+  HPLLR  + G+ + HGFS ++++SLA IC T LPP               T++  
Sbjct: 6   RRNRGHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPP-------------ETTSEQQ 65

Query: 81  AVEAFYAASGSRSPIPDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT 140
           AV +F+ AS ++ P  DEVAE + K G +E V ++R++L +L  R GTLLLCGSLC  ++
Sbjct: 66  AVNSFHVASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKS 125

Query: 141 WPYICNFSSMPLKNREEALRRWYKNKVF-TPIRAALASIRVLVLYTFFSRVDVNGGFPAW 200
           WP++  FS +PL  RE  LR W +   F  P R      +   L+ FFS+ D N   PA 
Sbjct: 126 WPFVLKFSQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPAL 185

Query: 201 EAIKYRPDIEE---NRSKSSDE--RPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDT 260
           EAI Y  D  E   N+   +DE  RPL+ GIIE  +E++ +  +SL +KG+ V  DD D 
Sbjct: 186 EAIGYCIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVARDDGDN 245

Query: 261 L-SIQCDVVVVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAG 320
           +  I+CD VVVGSG GGGVAAA LA +G KV+VLEKGNYFTA DYS LE PS+ +LYE G
Sbjct: 246 VYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKG 305

Query: 321 GIFTSDDGKMLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDA 380
           G+ T+ DGK ++LAGS VGGG+AVNWSASIRTP+HVLQEW+E  KI FFG+ EYQ AMD 
Sbjct: 306 GLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDE 365

Query: 381 VCKRLGVSEDCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGT 440
           V  R+GV+E C + GFQNQVLRKGCE LGL VE VPRNSP  HYCG CGYGCR G K GT
Sbjct: 366 VTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGT 425

Query: 441 DSTWLVDAVNHGAVIITNCKAERFIL-ERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKA 500
           D TWLVDAV +GAVI+T  KAERF+L +   +   RK +C+GV A+       KK  I+A
Sbjct: 426 DQTWLVDAVENGAVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEA 485

Query: 501 KATISACGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIIT 560
           + T+S+ G+LLTPPLM+SSGL+N +IGRNL LHPVLM WGYFP+  SEF GK YEGGIIT
Sbjct: 486 RVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFPEKDSEFSGKMYEGGIIT 545

Query: 561 SLHKVISEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDS 620
           S+H +    +ES  K I+E PL+GP S++ L PW+SG D K+RM+KY R +HL  +VRD 
Sbjct: 546 SVHHM--NDTESGCKAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 605

Query: 621 GSGAVRSEGRVSFNLSEEDRENLKRGLRQSLR 644
           GSG V  E  V++  +++DRENL+ GLRQ+LR
Sbjct: 606 GSGEVMMENEVTYRTTKKDRENLRAGLRQALR 622

BLAST of Carg16550 vs. TAIR 10
Match: AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 599.0 bits (1543), Expect = 4.6e-171
Identity = 311/626 (49.68%), Postives = 428/626 (68.37%), Query Frame = 0

Query: 27  PLLR-GTRSGKFTHGFSVAEMESLATICDTILPPLPLGSSNNNGSDEIDTADAAAVEAFY 86
           PLLR   +   F+HGFS +++++L++ICD I+PP+PL S N     ++   D  A+ +F+
Sbjct: 13  PLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLRND--ALLSFF 72

Query: 87  AASGSRSPI-PDEVAETVAKRGLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRT-WPYI 146
            +S S S + PDEVAE +A + +   V++VR+VL +L  RLGTLLLCG +C  +  WP++
Sbjct: 73  KSSSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFL 132

Query: 147 CNFSSMPLKNREEALRRWYKNKVFTPI-RAALASIRVLVLYTFFSRVDVNGGFPAWEAIK 206
             FS M L+ RE+ L+RW   + + P+ R     I+ + L+ +F+  + N   P W+AI 
Sbjct: 133 LKFSEMSLEKREKVLQRW-NTQWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAIN 192

Query: 207 YRPDIEENRSKSSDERPLQTGIIEPNNETESSFQKSLLQKGLRVTGD-DSDTLSIQCDVV 266
           Y  +I EN      ERPL  GIIE   E E + ++ ++ KGL+VT D + DT  I+CD V
Sbjct: 193 YSVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRERDTYKIECDAV 252

Query: 267 VVGSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDDGK 326
           VVGSGCGGGVAAA+LA SG +VVV+EKGNYF   DYS+LEGPS+ +L+E+  +  + DG+
Sbjct: 253 VVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFELFESNSLMMTHDGR 312

Query: 327 MLILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGVSE 386
              +AGSTVGGGS VNW+AS++TP+ +++EW+    I  + + +Y+ AM  VCKRLGV+E
Sbjct: 313 FRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYKAAMGIVCKRLGVTE 372

Query: 387 DCDEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVDAV 446
               EGFQNQ+LRKGCE LGL V  VPRNS   HYCGSC YGC  GEK+GTDSTWLVDAV
Sbjct: 373 KIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAV 432

Query: 447 NHGAVIITNCKAERFILERN----KTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISA 506
           N+ AVI+T CKAE+ IL  N    +    R+ +CLGV A++ S    KKLQI AK TI A
Sbjct: 433 NNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAASL-SHQTRKKLQINAKVTIVA 492

Query: 507 CGALLTPPLMISSGLRNKHIGRNLHLHPVLMAWGYFPDSTSEFKGKSYEGGIITSLHKVI 566
           CG+L TP L+ SSGL+N +I R LH+HP++MAWGYFP+  SE +G ++EG I+TSLH V 
Sbjct: 493 CGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNSELEGAAHEGEIVTSLHYVH 552

Query: 567 SEGSESNSKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVR 626
              S + + T +ETP +GPG+F+ L PW+SG D K+RM KY+R +H+  +VRD G G V+
Sbjct: 553 PMDSTTPNIT-LETPAIGPGTFAALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVK 612

Query: 627 SEGRVSFNLSEEDRENLKRGLRQSLR 644
            +  V + L++ D ENL  GL+Q+LR
Sbjct: 613 GD-IVKYRLTKADEENLTIGLKQALR 632

BLAST of Carg16550 vs. TAIR 10
Match: AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 480.3 bits (1235), Expect = 2.4e-135
Identity = 269/624 (43.11%), Postives = 382/624 (61.22%), Query Frame = 0

Query: 46  MESLATICDTILPPLPLGSSNNNGSDEIDTADAAAVEAFYAASGSRSPIPDEVAETVAKR 105
           MESL  ICDT      + S +++G   +D      V  +++AS S++  PD VA  +++R
Sbjct: 1   MESLVAICDTF-----ISSIDDSGVGHVDD----CVAGYFSASASQTGTPDRVARLMSER 60

Query: 106 GLTELVILVRMVLWLLGTRLGTLLLCGSLCFCRTWPYICNFSSMPLKNREEALRRWYKNK 165
                  ++R  LWLL T +G+L+LCG   F   +PY   F  +P K REE L  W  + 
Sbjct: 61  LHHPKKWILRAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNW-SSS 120

Query: 166 VFTPIRAALASIRVLVLYTFFSRVDVNGGFPAWEAIKYR---PD--------IEENRSKS 225
            F+ +R    +I+++    FF++VD  G   AW+AI Y    PD         EE + K 
Sbjct: 121 YFSLLRMLFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPDHSDHEVELNEEKKKKK 180

Query: 226 SDE--RPLQTGIIEPNNETESSFQKSLLQKGLRVTGDDSDT---------LSIQCDVVVV 285
            +E   PL  GI++  +  E + +K L  +G  V+    +T         + IQCD VVV
Sbjct: 181 PEEIFGPLYNGIVDLKSPRE-AVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVV 240

Query: 286 GSGCGGGVAAAVLASSGQKVVVLEKGNYFTASDYSSLEGPSLNQLYEAGGIFTSDDGKML 345
           GSG GGGVAA VLA +G KV+V+E GNY+  S  S LEG +++ +Y +GG+  + D  ++
Sbjct: 241 GSGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVV 300

Query: 346 ILAGSTVGGGSAVNWSASIRTPNHVLQEWAESHKIPFFGTSEYQIAMDAVCKRLGVSEDC 405
           ILAGSTVGGGS +NWSASI+TP HV++EWAE  K+  FG+  Y+ AMD VCKR+GV    
Sbjct: 301 ILAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGF 360

Query: 406 DEEGFQNQVLRKGCENLGLTVEKVPRNSPSGHYCGSCGYGCRRGEKQGTDSTWLVDAV-N 465
            EEGF N+VLRKGCE LGL V+ +PRN+PS HYCG C  GC++G+KQGT  TWLVD V +
Sbjct: 361 VEEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVES 420

Query: 466 HGAVIITNCKAERFILERNKTGGVRKSKCLGVLANVWSDNFTKKLQIKAKATISACGALL 525
              +I+  C+A   + +  +    +K K  GV      + +     ++++ TI ACGAL 
Sbjct: 421 DNGLILPGCQATEVMYDCEQG---KKKKATGVAFAFGEEIYV----VESRVTIVACGALR 480

Query: 526 TPPLMISSGLRNKHIGRNLHLHPVLMAWGYFP--DSTSEFKGKSYEGGIITSLHKVISEG 585
           TP L+  SGL+N +IGRNL LHPV+MAWG+FP  D   E K KSYEGGI+T++  V+ E 
Sbjct: 481 TPHLLKRSGLKNSNIGRNLCLHPVVMAWGWFPEEDKWPEKKKKSYEGGIMTAMSSVVIEE 540

Query: 586 SESN-SKTIIETPLLGPGSFSVLFPWISGLDTKQRMLKYSRISHLITIVRDSGSGAVRSE 644
           + S+  + +I+TP L PG FS + PW S  D K RMLK+SR +H+  ++RD G+G + S+
Sbjct: 541 THSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSK 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018802.10.0e+00100.00Long-chain-alcohol oxidase FAO1, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6582399.10.0e+0099.52Long-chain-alcohol oxidase FAO1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022924775.10.0e+0098.24long-chain-alcohol oxidase FAO1-like [Cucurbita moschata][more]
XP_023527426.10.0e+0097.44long-chain-alcohol oxidase FAO1-like [Cucurbita pepo subsp. pepo][more]
XP_022979358.10.0e+0096.47long-chain-alcohol oxidase FAO1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
B5WWZ84.6e-22462.94Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1[more]
Q9LW564.9e-20258.67Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=... [more]
B5WWZ96.0e-20055.73Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1[more]
Q94BP31.8e-18854.27Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 S... [more]
Q9ZWB96.5e-17049.68Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1EG060.0e+0098.24Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111432173 PE=3 SV... [more]
A0A6J1IVY90.0e+0096.47Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479108 PE=3 SV=1[more]
A0A5A7U0C12.2e-30683.01Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3AW606.5e-30683.01Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103483573 PE=3 SV=1[more]
A0A6J1IBI09.1e-30080.61Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111473170 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G23410.13.5e-20358.67fatty alcohol oxidase 3 [more]
AT4G28570.11.3e-18954.27Long-chain fatty alcohol dehydrogenase family protein [more]
AT1G03990.14.6e-17149.68Long-chain fatty alcohol dehydrogenase family protein [more]
AT4G19380.12.4e-13543.11Long-chain fatty alcohol dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 259..526
e-value: 1.4E-68
score: 231.4
IPR012400Long-chain-alcohol oxidasePIRSFPIRSF028937Lg_Ch_AOcoord: 28..645
e-value: 2.0E-242
score: 804.5
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 245..550
e-value: 2.4E-26
score: 94.5
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 255..539
NoneNo IPR availablePIRSRPIRSR028937-2PIRSR028937-2coord: 23..643
e-value: 3.0E-288
score: 956.1
NoneNo IPR availablePANTHERPTHR46056LONG-CHAIN-ALCOHOL OXIDASEcoord: 22..643
NoneNo IPR availablePANTHERPTHR46056:SF10LONG-CHAIN-ALCOHOL OXIDASE FAO3coord: 22..643

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16550-RACarg16550-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046577 long-chain-alcohol oxidase activity
molecular_function GO:0046593 mandelonitrile lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors