Carg16527 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16527
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMajor facilitator superfamily protein
LocationCarg_Chr14: 13246829 .. 13249171 (-)
RNA-Seq ExpressionCarg16527
SyntenyCarg16527
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTGTTCATTATTTGCCCCTGATTTTCCGTTTCCCTCTCTGAAACCCACGTTTCCCAGATCCCCACGAAGGCCACCGCTCTATTTTCCCAAGCACATTCTCTGTTTGCACCATCGAAACCACAATATCTTCGAAAACCCATCAAAACCCACAGGTATTTTCATCAACACGCTTGTGGAGAGCAACCCAGGTCATGTTTTGTATAGAGAGGCCAAGAAAAGAGAAGGGAATGGCAGAAAAAGAGAGTTTCCTCTTGTGGGTAGCTATCAAATGTTGCTGCTCTGTGGGCTTGGGTATTGGGTGCAGGGGTTTAGGTGCTTTCCATGGCTGGCTCTTAACTTTCACATGGCTCATTACCTTAATCTTCATCCTTCCATATTGCAACTCGTGCAGAATTCTGGAAACCTTCCTATGGTTGCTAAACCCTTGTATGGAATTCTGTCTGATGCTCTGTATATTGGTGGTGCTCGTAGAGTTCCTTATGTTTCAATTGGAGGTATGTTGGGTTTCTCTTTGAATTTCAACCATGTTCTTGTTCATTTCATTTCTGTATTTCTTGTTTGGTGTTCTTTTCTTCATTCTTTATTCTTTTCTTTTCCAGTTCATCCTGCATTAGATTCCCTTCCGCGTTCAGTTTAATCAATAATGTAACTATTACTCAATCTTATTCCAAAGTAAAGATGAGTTTGGTTAGGAATCACAAACCTCACAACGGTATGATATTGTCCACTTTAAGCATAAGCTCTCGTGGTTTTGCTTTGGGCTTTCCCATGTATTCCTTACTTATAAATCCATGATCACGAGGGACTCCCTCCCAACCATCCTCAACAATCCTCTTCTCGAACAAAGTACACTATAGAGCCTCCCACGAGGCCTATGAGCCCTTGAACAACTCCCCTTAATCAAGGCTCGACTCCTTCTCTTGAGTCACGTTTGACTATACTTTCGAGGCTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGGGTAAGTTAAGGGCATGATTCTGATACCATGTTAGGAATCACGGACCTCCACAATGGTATGATATTGTTCACGTGGTTTTGCTTTTGGTTTCCCCAAAAAGCCTCGTGCCAAGGGAGATGTATTCTTGACCCATTGTTTTGGGTATACTTATGTGCATTGTGATGAAGCCTAATTGAGAAGCTTCTCAGTGCTGTTGCAGGTCCTATCTTGGGGATTTCTGGCATTGATCCCTGTGGCAGGGGAAGCCCTTCCTGCTTTAATGGCATGTGTTCTTCTAAGTAATCTTGGAGCATCCATAACAGAAGTTGCAAAAGATGCACTTGTTGCAGAATTTGGCCAAACAAACAAAATTTGTGGCCTTCAATCTTATACATTTATGGCTTTAGCTGTTGGTGGTGTCTTAGGCAACTTCCTTGGTGGATATAACTTACTGAGTTCACCGCCTAGAAAGATGTTTCTTCTCTTTTCTATTCTACTCTCTCTCCAACTTTCAATCTCATCAGCAACTAGAGAGGAATCCCTTGGTTTGCTGCAACTTCCAGAGCCTGATGTTCATGGTAAATCAATCATGGAAAATGTTAGAAATCAGATGTCGGACCTCGTAACGGCTATTCGTGATCCGAGTATATTTCGACCGCTGGCTTGGATTGTGGCTTCCATTGCCATGGTCCCTCTTCTAACAGGCTCTATATTTTGCTATCAAACACAATGCCTTCATCTTGATCCTTCCATAATTGGGATGTCTAGAGTGGTTAGTCAACTGGTTCTTCTTTCGGTCACCGTACTTTACGACCGCTATTGGAAACGAGTATCCATGAAGAAGCTAATTGGCATGGTGCAGATTTTGTACGCGTCTTCCTTTCTCCTCGACATTGTTTTGGTGAATCAGATAAATCTAAAGCTGGGCATTTCCAATGAGTTGTTTGCTCTTTGTTTTTCGGGTTTGGCCGAAACCATTGCACAGTTTAAGCTTTTACCATTTTCAGTACTCTTTGCAAGCTTAAGTCCAAAAGGTTGTGAAGGATCTCTGATTTCATTTTTTGCATCGGCGGTGTGCTTGTCTTCGATAGTTGGTGGTTTTTTCGGCATCGGGTTGGCTTCTTTGATTGGAATAAGATCAGGAAACTACTCAAGCTTGCCTGTGGGGATTTTGATACAGTTCATTGCAGCTTTATTGCCATTGATCTGTATCCATTTTGTTCCCACGATATCACCCGTCGTCGAAAGAGAAAAGAAGAGAATAGTTAGTAGAAGATCTAGGAGAAATAGAAGGGTCGGACGAGTCGTGTATAGTGATTCTGTATATGTTTATCGACGCGAGAGGGAATCCGAGATGCAGGGGTGA

mRNA sequence

ATGATTTGTTCATTATTTGCCCCTGATTTTCCGTTTCCCTCTCTGAAACCCACGTTTCCCAGATCCCCACGAAGGCCACCGCTCTATTTTCCCAAGCACATTCTCTGTTTGCACCATCGAAACCACAATATCTTCGAAAACCCATCAAAACCCACAGGTATTTTCATCAACACGCTTGTGGAGAGCAACCCAGGTCATGTTTTGTATAGAGAGGCCAAGAAAAGAGAAGGGAATGGCAGAAAAAGAGAGTTTCCTCTTGTGGGTAGCTATCAAATGTTGCTGCTCTGTGGGCTTGGGTATTGGGTGCAGGGGTTTAGGTGCTTTCCATGGCTGGCTCTTAACTTTCACATGGCTCATTACCTTAATCTTCATCCTTCCATATTGCAACTCGTGCAGAATTCTGGAAACCTTCCTATGGTTGCTAAACCCTTGTATGGAATTCTGTCTGATGCTCTGTATATTGGTGGTGCTCGTAGAGTTCCTTATGTTTCAATTGGAGGGGAAGCCCTTCCTGCTTTAATGGCATGTGTTCTTCTAAGTAATCTTGGAGCATCCATAACAGAAGTTGCAAAAGATGCACTTGTTGCAGAATTTGGCCAAACAAACAAAATTTGTGGCCTTCAATCTTATACATTTATGGCTTTAGCTGTTGGTGGTGTCTTAGGCAACTTCCTTGGTGGATATAACTTACTGAGTTCACCGCCTAGAAAGATGTTTCTTCTCTTTTCTATTCTACTCTCTCTCCAACTTTCAATCTCATCAGCAACTAGAGAGGAATCCCTTGGTTTGCTGCAACTTCCAGAGCCTGATGTTCATGGTAAATCAATCATGGAAAATGTTAGAAATCAGATGTCGGACCTCGTAACGGCTATTCGTGATCCGAGTATATTTCGACCGCTGGCTTGGATTGTGGCTTCCATTGCCATGGTCCCTCTTCTAACAGGCTCTATATTTTGCTATCAAACACAATGCCTTCATCTTGATCCTTCCATAATTGGGATGTCTAGAGTGGTTAGTCAACTGGTTCTTCTTTCGGTCACCGTACTTTACGACCGCTATTGGAAACGAGTATCCATGAAGAAGCTAATTGGCATGGTGCAGATTTTGTACGCGTCTTCCTTTCTCCTCGACATTGTTTTGGTGAATCAGATAAATCTAAAGCTGGGCATTTCCAATGAGTTGTTTGCTCTTTGTTTTTCGGGTTTGGCCGAAACCATTGCACAGTTTAAGCTTTTACCATTTTCAGTACTCTTTGCAAGCTTAAGTCCAAAAGGTTGTGAAGGATCTCTGATTTCATTTTTTGCATCGGCGGTGTGCTTGTCTTCGATAGTTGGTGGTTTTTTCGGCATCGGGTTGGCTTCTTTGATTGGAATAAGATCAGGAAACTACTCAAGCTTGCCTGTGGGGATTTTGATACAGTTCATTGCAGCTTTATTGCCATTGATCTGTATCCATTTTGTTCCCACGATATCACCCGTCGTCGAAAGAGAAAAGAAGAGAATAGTTAGTAGAAGATCTAGGAGAAATAGAAGGGTCGGACGAGTCGTGTATAGTGATTCTGTATATGTTTATCGACGCGAGAGGGAATCCGAGATGCAGGGGTGA

Coding sequence (CDS)

ATGATTTGTTCATTATTTGCCCCTGATTTTCCGTTTCCCTCTCTGAAACCCACGTTTCCCAGATCCCCACGAAGGCCACCGCTCTATTTTCCCAAGCACATTCTCTGTTTGCACCATCGAAACCACAATATCTTCGAAAACCCATCAAAACCCACAGGTATTTTCATCAACACGCTTGTGGAGAGCAACCCAGGTCATGTTTTGTATAGAGAGGCCAAGAAAAGAGAAGGGAATGGCAGAAAAAGAGAGTTTCCTCTTGTGGGTAGCTATCAAATGTTGCTGCTCTGTGGGCTTGGGTATTGGGTGCAGGGGTTTAGGTGCTTTCCATGGCTGGCTCTTAACTTTCACATGGCTCATTACCTTAATCTTCATCCTTCCATATTGCAACTCGTGCAGAATTCTGGAAACCTTCCTATGGTTGCTAAACCCTTGTATGGAATTCTGTCTGATGCTCTGTATATTGGTGGTGCTCGTAGAGTTCCTTATGTTTCAATTGGAGGGGAAGCCCTTCCTGCTTTAATGGCATGTGTTCTTCTAAGTAATCTTGGAGCATCCATAACAGAAGTTGCAAAAGATGCACTTGTTGCAGAATTTGGCCAAACAAACAAAATTTGTGGCCTTCAATCTTATACATTTATGGCTTTAGCTGTTGGTGGTGTCTTAGGCAACTTCCTTGGTGGATATAACTTACTGAGTTCACCGCCTAGAAAGATGTTTCTTCTCTTTTCTATTCTACTCTCTCTCCAACTTTCAATCTCATCAGCAACTAGAGAGGAATCCCTTGGTTTGCTGCAACTTCCAGAGCCTGATGTTCATGGTAAATCAATCATGGAAAATGTTAGAAATCAGATGTCGGACCTCGTAACGGCTATTCGTGATCCGAGTATATTTCGACCGCTGGCTTGGATTGTGGCTTCCATTGCCATGGTCCCTCTTCTAACAGGCTCTATATTTTGCTATCAAACACAATGCCTTCATCTTGATCCTTCCATAATTGGGATGTCTAGAGTGGTTAGTCAACTGGTTCTTCTTTCGGTCACCGTACTTTACGACCGCTATTGGAAACGAGTATCCATGAAGAAGCTAATTGGCATGGTGCAGATTTTGTACGCGTCTTCCTTTCTCCTCGACATTGTTTTGGTGAATCAGATAAATCTAAAGCTGGGCATTTCCAATGAGTTGTTTGCTCTTTGTTTTTCGGGTTTGGCCGAAACCATTGCACAGTTTAAGCTTTTACCATTTTCAGTACTCTTTGCAAGCTTAAGTCCAAAAGGTTGTGAAGGATCTCTGATTTCATTTTTTGCATCGGCGGTGTGCTTGTCTTCGATAGTTGGTGGTTTTTTCGGCATCGGGTTGGCTTCTTTGATTGGAATAAGATCAGGAAACTACTCAAGCTTGCCTGTGGGGATTTTGATACAGTTCATTGCAGCTTTATTGCCATTGATCTGTATCCATTTTGTTCCCACGATATCACCCGTCGTCGAAAGAGAAAAGAAGAGAATAGTTAGTAGAAGATCTAGGAGAAATAGAAGGGTCGGACGAGTCGTGTATAGTGATTCTGTATATGTTTATCGACGCGAGAGGGAATCCGAGATGCAGGGGTGA

Protein sequence

MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLVESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERESEMQG
Homology
BLAST of Carg16527 vs. NCBI nr
Match: KAG7018825.1 (putative folate-biopterin transporter 9, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1037.7 bits (2682), Expect = 3.4e-299
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV
Sbjct: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGGEALPALMACVLLS 180
           LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGGEALPALMACVLLS
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGGEALPALMACVLLS 180

Query: 181 NLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFL 240
           NLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFL
Sbjct: 181 NLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFL 240

Query: 241 LFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPL 300
           LFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPL
Sbjct: 241 LFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPL 300

Query: 301 AWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMK 360
           AWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMK
Sbjct: 301 AWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMK 360

Query: 361 KLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFAS 420
           KLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFAS
Sbjct: 361 KLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFAS 420

Query: 421 LSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLP 480
           LSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLP
Sbjct: 421 LSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLP 480

Query: 481 LICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERESEMQG 535
           LICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERESEMQG
Sbjct: 481 LICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERESEMQG 534

BLAST of Carg16527 vs. NCBI nr
Match: KAG6582427.1 (putative folate-biopterin transporter 9, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1026.9 bits (2654), Expect = 6.1e-296
Identity = 534/551 (96.91%), Postives = 534/551 (96.91%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV
Sbjct: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG              
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL
Sbjct: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 535
           SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

BLAST of Carg16527 vs. NCBI nr
Match: XP_022924744.1 (probable folate-biopterin transporter 9, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.3e-294
Identity = 532/551 (96.55%), Postives = 532/551 (96.55%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRN NIFENPSKPTGIFINTLV
Sbjct: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG              
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL
Sbjct: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 535
           SSLPVGILIQFIAALLPLICIHFVPT SPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTTSPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

BLAST of Carg16527 vs. NCBI nr
Match: XP_023528713.1 (probable folate-biopterin transporter 9, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.7e-293
Identity = 531/551 (96.37%), Postives = 531/551 (96.37%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSL APDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRN NIFENPSKPTGIFINTLV
Sbjct: 1   MICSLIAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG              
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL
Sbjct: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 535
           SSLPVGILIQFIAALLPLICIHFVPT SPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTTSPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

BLAST of Carg16527 vs. NCBI nr
Match: XP_022980264.1 (probable folate-biopterin transporter 9, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 1016.5 bits (2627), Expect = 8.2e-293
Identity = 528/551 (95.83%), Postives = 530/551 (96.19%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSL APDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRN NIFENPSKPTGIFINTLV
Sbjct: 1   MICSLIAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNPGHVLYRE KKREGNGRKREFPLVGSYQMLL+CGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREVKKREGNGRKREFPLVGSYQMLLICGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG              
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           MSDL+TAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL
Sbjct: 301 MSDLITAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 535
           SSLPVGILIQFIAALLPLICIHFVPTISP VEREKKRIVSRRSRRNRRVGRVVYSDSVYV
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTISPAVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

BLAST of Carg16527 vs. ExPASy Swiss-Prot
Match: F4I5Q2 (Probable folate-biopterin transporter 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g04570 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-148
Identity = 300/533 (56.29%), Postives = 367/533 (68.86%), Query Frame = 0

Query: 22  SPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLVESNPGHVLYREAKKREGNGRK 81
           S +  P  F K  L   HR     E  S    +F+                ++R  N   
Sbjct: 11  SIQNSPTKFLKPPLSSIHRQQQQQERQSNNNTLFMGEARPRRATTFACLSIRRRRNNNGV 70

Query: 82  RE------FPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSG 141
            E      F  VG   + +LCGLGYWVQG RCFPWLALNFHM H L L PS LQLVQ S 
Sbjct: 71  SEFEETARFEQVGGKGISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSC 130

Query: 142 NLPMVAKPLYGILSDALYIGGARRVPYVSIG-----------------GEALPALMACVL 201
           +LPMVAKPLYG+LSD LYIG  RRVPY++IG                  E LP+L+ACVL
Sbjct: 131 SLPMVAKPLYGVLSDVLYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVL 190

Query: 202 LSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKM 261
           LSNLGASITEVAKDALVAE+G   +I GLQSY  MA A GGVLGN LGGY LL++PP+  
Sbjct: 191 LSNLGASITEVAKDALVAEYGLRYRINGLQSYALMASAAGGVLGNLLGGYLLLTTPPKIS 250

Query: 262 FLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFR 321
           FL+FS LLSLQL +S +++EES GL ++ E      S++E+V+ Q+S+L  AI+   I +
Sbjct: 251 FLVFSALLSLQLVVSLSSKEESFGLPRIAET----SSVLESVKKQISNLKEAIQADEISQ 310

Query: 322 PLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVS 381
           PL W V SIAMVPLL+GS+FCYQTQ L+LDPS+IGMS+V+ QL+LL +TV+YDRY K + 
Sbjct: 311 PLIWAVVSIAMVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLP 370

Query: 382 MKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLF 441
           M+ LI ++Q+LY  S LLD +LV QINL  GISNE++ LCFS LAE +AQFK+LPF+V  
Sbjct: 371 MRPLIHIIQLLYGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRL 430

Query: 442 ASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAAL 501
           AS+ P+GCEGS+ SF AS +CLS IV  F G+GLA+LIGI S NYS+L  GILIQ +AAL
Sbjct: 431 ASMCPQGCEGSVTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAAL 490

Query: 502 LPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERESE 532
            PL  +H VP   PV+E+E KR +S+RSRRNRRVGRVV  +SV  YRRERESE
Sbjct: 491 APLCFMHLVPMSEPVIEKEGKRGISKRSRRNRRVGRVVDKESV-TYRRERESE 538

BLAST of Carg16527 vs. ExPASy Swiss-Prot
Match: O22780 (Probable folate-biopterin transporter 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g33280 PE=3 SV=2)

HSP 1 Score: 522.7 bits (1345), Expect = 4.9e-147
Identity = 278/457 (60.83%), Postives = 348/457 (76.15%), Query Frame = 0

Query: 91  QMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSD 150
           Q +LLC LGYWVQG RCF WLALNFHMAH LNL PS LQLVQ + +LPMVAKPLYG+LSD
Sbjct: 83  QTVLLCALGYWVQGLRCFSWLALNFHMAHCLNLKPSTLQLVQYTASLPMVAKPLYGVLSD 142

Query: 151 ALYIGGARRVPYVSIG-----------------GEALPALMACVLLSNLGASITEVAKDA 210
            LYIGGARRVPY+S+G                  E LP+LMA +LLSNLGASITEV++DA
Sbjct: 143 VLYIGGARRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSNLGASITEVSQDA 202

Query: 211 LVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSIS 270
           LVAE+G   +I GLQSY  MA AVGG+LGN LGGY LL +PPR +FL F+ LLSLQL +S
Sbjct: 203 LVAEYGLRYQINGLQSYALMASAVGGILGNLLGGYCLLKTPPRILFLAFTALLSLQLIVS 262

Query: 271 SATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPLAWIVASIAMVPLL 330
            +++EES+ L ++ E      S++  V+ Q  DL   ++   I +PL WIV+SIA+VPLL
Sbjct: 263 LSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQVDEISQPLIWIVSSIALVPLL 322

Query: 331 TGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASS 390
           +GS+FCYQTQ L+LDPS+IGMS+V+ QL+LL +TV+YDRYWK++ M+ LI +VQ+LYA S
Sbjct: 323 SGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYWKKLPMRALIHIVQLLYAFS 382

Query: 391 FLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISF 450
            L D +LV QINL  GISN  F LCFS +AE +AQFK+LPFSVL A++ P GCEGS+ SF
Sbjct: 383 LLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKILPFSVLLANMCPGGCEGSITSF 442

Query: 451 FASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLICIHFVPTISPV 510
            AS +CLSS+V GF G+G+A++IGI S NYS+LP GILIQ +AAL+PL  IH+VP + P 
Sbjct: 443 LASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILIQSLAALVPLWFIHYVPMLEPG 502

Query: 511 VEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERES 531
            ERE KR +S++SRRNRRVGRV+  +S + YRRERE+
Sbjct: 503 FEREGKRAMSKKSRRNRRVGRVIGQES-FAYRRERET 538

BLAST of Carg16527 vs. ExPASy Swiss-Prot
Match: Q9XIQ7 (Probable folate-biopterin transporter 7 OS=Arabidopsis thaliana OX=3702 GN=At1g64890 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 9.7e-79
Identity = 170/426 (39.91%), Postives = 256/426 (60.09%), Query Frame = 0

Query: 95  LCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSDALYI 154
           L G G++VQGFR FPWL  NF +   L ++PS+LQL+QNS NLPMVAKP+YG++SD++Y 
Sbjct: 22  LLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVSDSVYF 81

Query: 155 GGARRVPYVSIG-----------------GEALPALMACVLLSNLGASITEVAKDALVAE 214
            G  R+PY+++G                   ++ AL   +LLSNLGAS+ EVA DA+VAE
Sbjct: 82  FGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVANDAIVAE 141

Query: 215 FGQ----TNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSIS 274
            G+      +   L S+ +M  ++GG+LGN LGG  + +   +  FL+F IL  LQ  ++
Sbjct: 142 SGKQKTSETQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFGILALLQFLVT 201

Query: 275 SATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPLAWIVASIAMVPLL 334
              RE+SL L + P P          +R  +SDL   +R P I   +AWI  S A+VP+L
Sbjct: 202 INIREKSLNLPENPSP-------AGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVVPVL 261

Query: 335 TGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASS 394
           TG++F YQT+ L +D S++G+S+V  Q+ +L     Y+R+ K +  +KL+  +Q+  A  
Sbjct: 262 TGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTIAFF 321

Query: 395 FLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISF 454
            + D++ V  +   LG+S+ ++ L FSG  ET+  FK+LPF+VL A L P GCEGSL++F
Sbjct: 322 VISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSLMAF 381

Query: 455 FASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLICIHFVPTISPV 500
             SA+ L+ IV G+ GI LAS +G+   ++S    G+ I+     +PLI   ++   +  
Sbjct: 382 VMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDEAET 440

BLAST of Carg16527 vs. ExPASy Swiss-Prot
Match: Q8RWQ5 (Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g54860 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.5e-52
Identity = 152/468 (32.48%), Postives = 230/468 (49.15%), Query Frame = 0

Query: 93  LLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSDAL 152
           L L  L Y+ QGFR F W A+++ +   L L PS  Q V +    P   KPLYGI+SD +
Sbjct: 17  LWLVCLIYFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCI 76

Query: 153 YIGGARRVPYV-----------------SIGGEALPALMACVLLSNLGASITEVAKDALV 212
            IGG +R PY+                 S    +   LM  + + NLG+++ +V  DA++
Sbjct: 77  PIGGKKRTPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMI 136

Query: 213 AEFGQTNKIC---GLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSI 272
           AE  +  K      LQS ++ A+AVGGV G+ LGGY L +     +FLLF++L +LQL  
Sbjct: 137 AEAVRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLS 196

Query: 273 SSATREESLGLLQLPE-------------------------------------PDVHGKS 332
            +   E       LPE                                      D +GKS
Sbjct: 197 CALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKS 256

Query: 333 IMENVRNQMSDLVT---------------AIRDPSIFRPLAWIVASIAMVPLLTGSIFCY 392
             E  + Q   L +               A + P I RP+AW   +   VP L+  +F Y
Sbjct: 257 --ETQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYY 316

Query: 393 QTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVL 452
           QT+ L LD + +G +RVV  L L+  T +Y+RY + ++++K +    I  + + LLD+VL
Sbjct: 317 QTEVLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVL 376

Query: 453 VNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCL 489
           V++ N+  G+S++   L  S L + I Q K +PF +L   L P G EG+L + F S   L
Sbjct: 377 VSRANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNL 436

BLAST of Carg16527 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 2.2e-30
Identity = 110/413 (26.63%), Postives = 204/413 (49.39%), Query Frame = 0

Query: 100 YWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARR 159
           Y+VQG      LA++F++   L+L P+   ++    +LP + KPLYG +SD++ + G RR
Sbjct: 134 YFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFISDSVPLFGYRR 193

Query: 160 VPYVSIGG--------------EALPALMACVLLSNLGASITEVAKDALVAE--FGQTNK 219
             Y+ + G              ++  +   C+LL +L  + ++V  D++V E   G++  
Sbjct: 194 RSYLVLSGLLGAFSWSLMAGFVDSKYSAAFCILLGSLSVAFSDVVVDSMVVERARGESQS 253

Query: 220 ICG-LQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESL- 279
           + G LQS  + + A GG++ ++  G  + S   R +F + ++L  +  +++    E+ + 
Sbjct: 254 VSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTALLPLITSAVAVLVNEQRVV 313

Query: 280 --GLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFC 339
                Q     +     ++  +  M  L  AI+ P++F P  +I    A  P    ++F 
Sbjct: 314 RPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPTLFIFLWQA-TPHSDSAMFY 373

Query: 340 YQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIV 399
           + T  L   P  +G  ++V+ +  L    LY+ + K V ++K+  +  I      +  ++
Sbjct: 374 FTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLRKIFLVTTIFGTGLGMTQVI 433

Query: 400 LVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVC 459
           LV+  N +LGIS+E FA+  S +   +AQ   +P  VL A L P+G E +L +   S   
Sbjct: 434 LVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAARLCPEGMEATLFATLMSISN 493

Query: 460 LSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLICIHFVPTISP 493
             S++GG  G GL    GI   ++ +L   I++  +++LLPL  +  +P  SP
Sbjct: 494 GGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLPLPLLGLLPRDSP 545

BLAST of Carg16527 vs. ExPASy TrEMBL
Match: A0A6J1EA28 (probable folate-biopterin transporter 9, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111432152 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.6e-294
Identity = 532/551 (96.55%), Postives = 532/551 (96.55%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRN NIFENPSKPTGIFINTLV
Sbjct: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG              
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL
Sbjct: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 535
           SSLPVGILIQFIAALLPLICIHFVPT SPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTTSPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

BLAST of Carg16527 vs. ExPASy TrEMBL
Match: A0A6J1IYT4 (probable folate-biopterin transporter 9, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479684 PE=3 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.0e-293
Identity = 528/551 (95.83%), Postives = 530/551 (96.19%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSL APDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRN NIFENPSKPTGIFINTLV
Sbjct: 1   MICSLIAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNPGHVLYRE KKREGNGRKREFPLVGSYQMLL+CGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREVKKREGNGRKREFPLVGSYQMLLICGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG              
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           MSDL+TAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL
Sbjct: 301 MSDLITAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 535
           SSLPVGILIQFIAALLPLICIHFVPTISP VEREKKRIVSRRSRRNRRVGRVVYSDSVYV
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTISPAVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

BLAST of Carg16527 vs. ExPASy TrEMBL
Match: A0A1S3AX05 (probable folate-biopterin transporter 9, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483535 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 5.1e-256
Identity = 469/551 (85.12%), Postives = 495/551 (89.84%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MI SL +P+FPFPSLK    R P +P L FPK ILC HHRN NIFEN SKP GIFI T++
Sbjct: 1   MISSLISPNFPFPSLKSNSSRPPPKPSLSFPKPILCFHHRNPNIFENSSKPIGIFIKTIL 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNP  VL+RE KKREGNG KR   LVGS QMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPDRVLFREVKKREGNGGKRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPS+LQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPY+SIG              
Sbjct: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYISIGVLLQVLSWGSLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAE+GQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           F+GGYNLLSSPPRKMFLLFSILLSLQLS+S ATREESLGL+QLPEPDVHGKSIMENVRNQ
Sbjct: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLVQLPEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           +S+LVT I DPS+ RPLAWIVASIA+VPLL+GSIFCYQTQCL+LDPSIIGMSRVVSQLVL
Sbjct: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDR+WKRV MKKLIGMVQILY+ SFLLDIVLV QINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYSFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIV GF GIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFLGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 535
           SSLPVGILIQFIAALLPL CIHFVP  SPV+EREKKR++SRRSR+NRRVGRV+YS SV  
Sbjct: 481 SSLPVGILIQFIAALLPLTCIHFVPMKSPVLEREKKRVMSRRSRKNRRVGRVMYSGSVCA 540

BLAST of Carg16527 vs. ExPASy TrEMBL
Match: A0A6J1DZL8 (probable folate-biopterin transporter 9, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111024552 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.4e-253
Identity = 465/550 (84.55%), Postives = 492/550 (89.45%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MICSL AP  PFPSLKP F R P       PK ILCLHHRN N  + PSKP GIF NTL+
Sbjct: 1   MICSLIAPGNPFPSLKPIFARPP-------PKPILCLHHRNPNSIQTPSKPIGIFTNTLL 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ++NP   LYRE +KREGNGR+R   LVGS++MLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  QNNPARGLYREGEKREGNGRRRGSSLVGSHRMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPS LQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG              
Sbjct: 121 LNLHPSTLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGSLALI 180

Query: 181 ---GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240
              GEALPALMACVLLSNLGASITEVAKDALVAE+GQT+KICGLQSYTFM LAVGG+LGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTHKICGLQSYTFMGLAVGGILGN 240

Query: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           F+GGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQL EPDVHGKSIMENVRNQ
Sbjct: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLSEPDVHGKSIMENVRNQ 300

Query: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360
           ++DLVTA+ D S+F PL WIVASIAMVP+L+GSIFCYQTQCLHLDPSIIGMSRVVSQLVL
Sbjct: 301 INDLVTAVSDQSVFLPLTWIVASIAMVPVLSGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420
           LSVTVLYDR+WKRVSMKKLIGMVQILYASS L D+VLV QINLKLGISNE+FALCFSGLA
Sbjct: 361 LSVTVLYDRHWKRVSMKKLIGMVQILYASSLLFDLVLVKQINLKLGISNEMFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSV+FA+LSPKGCEGSLISFFASAVCLSSIV GFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVIFANLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 534
           SSLPVGILIQFIAALLPLI IHFVPT SPVVEREKKR++SRRSR+NRRVGRV+YS SVYV
Sbjct: 481 SSLPVGILIQFIAALLPLIWIHFVPTTSPVVEREKKRVMSRRSRKNRRVGRVMYSGSVYV 540

BLAST of Carg16527 vs. ExPASy TrEMBL
Match: A0A0A0L6X9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G133990 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 9.3e-250
Identity = 463/560 (82.68%), Postives = 490/560 (87.50%), Query Frame = 0

Query: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLV 60
           MI SL +P+FPFPSLK  F R   +  L F K +LC HHRN NIFENPSKP  IFI T++
Sbjct: 1   MISSLISPNFPFPSLKSNFARP--KTSLSFHKPVLCFHHRNPNIFENPSKPIAIFIKTVL 60

Query: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           ESNP  VL+RE KKREGNG +R   LVGS QMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIG-------------- 180
           LNLHPS+LQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPY+SIG              
Sbjct: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYISIGVGLRIQTLHLQRVV 180

Query: 181 ------------GEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMA 240
                       GEALPALMACVLLSNLGASITEVAKDALVAE+GQTNKICGLQSYTFMA
Sbjct: 181 LSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMA 240

Query: 241 LAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGK 300
           LAVGGVLGNF+GGYNLLSSPPRKMFLLFSILLSLQLS+S ATREESLGLLQLPEPDV GK
Sbjct: 241 LAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVQGK 300

Query: 301 SIMENVRNQMSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGM 360
           SIMENVRNQ+S+LVT I DPS+ RPLAWIVASIA+VPLL+GSIFCYQTQCL+LDPSIIGM
Sbjct: 301 SIMENVRNQISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSIIGM 360

Query: 361 SRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNEL 420
           SRVVSQLVLLSVTVLYDR+WKRV MKKLIGMVQILYA SFLLDIVL+ QINLKLGISNEL
Sbjct: 361 SRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYALSFLLDIVLMKQINLKLGISNEL 420

Query: 421 FALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLAS 480
           FALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIV GF GIGLAS
Sbjct: 421 FALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFLGIGLAS 480

Query: 481 LIGIRSGNYSSLPVGILIQFIAALLPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGR 535
           LIGI SGNYSSLPVGILIQFIAAL PL CIHFVP  SPV+EREKKR++SRRSR+NRRVGR
Sbjct: 481 LIGIGSGNYSSLPVGILIQFIAALFPLTCIHFVPMKSPVLEREKKRVMSRRSRKNRRVGR 540

BLAST of Carg16527 vs. TAIR 10
Match: AT1G04570.1 (Major facilitator superfamily protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.8e-149
Identity = 300/533 (56.29%), Postives = 367/533 (68.86%), Query Frame = 0

Query: 22  SPRRPPLYFPKHILCLHHRNHNIFENPSKPTGIFINTLVESNPGHVLYREAKKREGNGRK 81
           S +  P  F K  L   HR     E  S    +F+                ++R  N   
Sbjct: 11  SIQNSPTKFLKPPLSSIHRQQQQQERQSNNNTLFMGEARPRRATTFACLSIRRRRNNNGV 70

Query: 82  RE------FPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSG 141
            E      F  VG   + +LCGLGYWVQG RCFPWLALNFHM H L L PS LQLVQ S 
Sbjct: 71  SEFEETARFEQVGGKGISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSC 130

Query: 142 NLPMVAKPLYGILSDALYIGGARRVPYVSIG-----------------GEALPALMACVL 201
           +LPMVAKPLYG+LSD LYIG  RRVPY++IG                  E LP+L+ACVL
Sbjct: 131 SLPMVAKPLYGVLSDVLYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVL 190

Query: 202 LSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKM 261
           LSNLGASITEVAKDALVAE+G   +I GLQSY  MA A GGVLGN LGGY LL++PP+  
Sbjct: 191 LSNLGASITEVAKDALVAEYGLRYRINGLQSYALMASAAGGVLGNLLGGYLLLTTPPKIS 250

Query: 262 FLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFR 321
           FL+FS LLSLQL +S +++EES GL ++ E      S++E+V+ Q+S+L  AI+   I +
Sbjct: 251 FLVFSALLSLQLVVSLSSKEESFGLPRIAET----SSVLESVKKQISNLKEAIQADEISQ 310

Query: 322 PLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVS 381
           PL W V SIAMVPLL+GS+FCYQTQ L+LDPS+IGMS+V+ QL+LL +TV+YDRY K + 
Sbjct: 311 PLIWAVVSIAMVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLP 370

Query: 382 MKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLF 441
           M+ LI ++Q+LY  S LLD +LV QINL  GISNE++ LCFS LAE +AQFK+LPF+V  
Sbjct: 371 MRPLIHIIQLLYGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRL 430

Query: 442 ASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAAL 501
           AS+ P+GCEGS+ SF AS +CLS IV  F G+GLA+LIGI S NYS+L  GILIQ +AAL
Sbjct: 431 ASMCPQGCEGSVTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAAL 490

Query: 502 LPLICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERESE 532
            PL  +H VP   PV+E+E KR +S+RSRRNRRVGRVV  +SV  YRRERESE
Sbjct: 491 APLCFMHLVPMSEPVIEKEGKRGISKRSRRNRRVGRVVDKESV-TYRRERESE 538

BLAST of Carg16527 vs. TAIR 10
Match: AT2G33280.1 (Major facilitator superfamily protein )

HSP 1 Score: 447.6 bits (1150), Expect = 1.4e-125
Identity = 242/409 (59.17%), Postives = 309/409 (75.55%), Query Frame = 0

Query: 139 MVAKPLYGILSDALYIGGARRVPYVSIG-----------------GEALPALMACVLLSN 198
           MVAKPLYG+LSD LYIGGARRVPY+S+G                  E LP+LMA +LLSN
Sbjct: 1   MVAKPLYGVLSDVLYIGGARRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSN 60

Query: 199 LGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLL 258
           LGASITEV++DALVAE+G   +I GLQSY  MA AVGG+LGN LGGY LL +PPR +FL 
Sbjct: 61  LGASITEVSQDALVAEYGLRYQINGLQSYALMASAVGGILGNLLGGYCLLKTPPRILFLA 120

Query: 259 FSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPLA 318
           F+ LLSLQL +S +++EES+ L ++ E      S++  V+ Q  DL   ++   I +PL 
Sbjct: 121 FTALLSLQLIVSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQVDEISQPLI 180

Query: 319 WIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKK 378
           WIV+SIA+VPLL+GS+FCYQTQ L+LDPS+IGMS+V+ QL+LL +TV+YDRYWK++ M+ 
Sbjct: 181 WIVSSIALVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYWKKLPMRA 240

Query: 379 LIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASL 438
           LI +VQ+LYA S L D +LV QINL  GISN  F LCFS +AE +AQFK+LPFSVL A++
Sbjct: 241 LIHIVQLLYAFSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKILPFSVLLANM 300

Query: 439 SPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPL 498
            P GCEGS+ SF AS +CLSS+V GF G+G+A++IGI S NYS+LP GILIQ +AAL+PL
Sbjct: 301 CPGGCEGSITSFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILIQSLAALVPL 360

Query: 499 ICIHFVPTISPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYVYRRERES 531
             IH+VP + P  ERE KR +S++SRRNRRVGRV+  +S + YRRERE+
Sbjct: 361 WFIHYVPMLEPGFEREGKRAMSKKSRRNRRVGRVIGQES-FAYRRERET 408

BLAST of Carg16527 vs. TAIR 10
Match: AT1G64890.1 (Major facilitator superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 6.9e-80
Identity = 170/426 (39.91%), Postives = 256/426 (60.09%), Query Frame = 0

Query: 95  LCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSDALYI 154
           L G G++VQGFR FPWL  NF +   L ++PS+LQL+QNS NLPMVAKP+YG++SD++Y 
Sbjct: 22  LLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVSDSVYF 81

Query: 155 GGARRVPYVSIG-----------------GEALPALMACVLLSNLGASITEVAKDALVAE 214
            G  R+PY+++G                   ++ AL   +LLSNLGAS+ EVA DA+VAE
Sbjct: 82  FGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVANDAIVAE 141

Query: 215 FGQ----TNKICGLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSIS 274
            G+      +   L S+ +M  ++GG+LGN LGG  + +   +  FL+F IL  LQ  ++
Sbjct: 142 SGKQKTSETQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFGILALLQFLVT 201

Query: 275 SATREESLGLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPLAWIVASIAMVPLL 334
              RE+SL L + P P          +R  +SDL   +R P I   +AWI  S A+VP+L
Sbjct: 202 INIREKSLNLPENPSP-------AGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVVPVL 261

Query: 335 TGSIFCYQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASS 394
           TG++F YQT+ L +D S++G+S+V  Q+ +L     Y+R+ K +  +KL+  +Q+  A  
Sbjct: 262 TGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTIAFF 321

Query: 395 FLLDIVLVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISF 454
            + D++ V  +   LG+S+ ++ L FSG  ET+  FK+LPF+VL A L P GCEGSL++F
Sbjct: 322 VISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSLMAF 381

Query: 455 FASAVCLSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLICIHFVPTISPV 500
             SA+ L+ IV G+ GI LAS +G+   ++S    G+ I+     +PLI   ++   +  
Sbjct: 382 VMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDEAET 440

BLAST of Carg16527 vs. TAIR 10
Match: AT5G54860.1 (Major facilitator superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 2.5e-53
Identity = 152/468 (32.48%), Postives = 230/468 (49.15%), Query Frame = 0

Query: 93  LLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSDAL 152
           L L  L Y+ QGFR F W A+++ +   L L PS  Q V +    P   KPLYGI+SD +
Sbjct: 17  LWLVCLIYFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCI 76

Query: 153 YIGGARRVPYV-----------------SIGGEALPALMACVLLSNLGASITEVAKDALV 212
            IGG +R PY+                 S    +   LM  + + NLG+++ +V  DA++
Sbjct: 77  PIGGKKRTPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMI 136

Query: 213 AEFGQTNKIC---GLQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSI 272
           AE  +  K      LQS ++ A+AVGGV G+ LGGY L +     +FLLF++L +LQL  
Sbjct: 137 AEAVRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLS 196

Query: 273 SSATREESLGLLQLPE-------------------------------------PDVHGKS 332
            +   E       LPE                                      D +GKS
Sbjct: 197 CALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKS 256

Query: 333 IMENVRNQMSDLVT---------------AIRDPSIFRPLAWIVASIAMVPLLTGSIFCY 392
             E  + Q   L +               A + P I RP+AW   +   VP L+  +F Y
Sbjct: 257 --ETQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYY 316

Query: 393 QTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVL 452
           QT+ L LD + +G +RVV  L L+  T +Y+RY + ++++K +    I  + + LLD+VL
Sbjct: 317 QTEVLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVL 376

Query: 453 VNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCL 489
           V++ N+  G+S++   L  S L + I Q K +PF +L   L P G EG+L + F S   L
Sbjct: 377 VSRANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNL 436

BLAST of Carg16527 vs. TAIR 10
Match: AT2G32040.1 (Major facilitator superfamily protein )

HSP 1 Score: 135.2 bits (339), Expect = 1.6e-31
Identity = 110/413 (26.63%), Postives = 204/413 (49.39%), Query Frame = 0

Query: 100 YWVQGFRCFPWLALNFHMAHYLNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARR 159
           Y+VQG      LA++F++   L+L P+   ++    +LP + KPLYG +SD++ + G RR
Sbjct: 134 YFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFISDSVPLFGYRR 193

Query: 160 VPYVSIGG--------------EALPALMACVLLSNLGASITEVAKDALVAE--FGQTNK 219
             Y+ + G              ++  +   C+LL +L  + ++V  D++V E   G++  
Sbjct: 194 RSYLVLSGLLGAFSWSLMAGFVDSKYSAAFCILLGSLSVAFSDVVVDSMVVERARGESQS 253

Query: 220 ICG-LQSYTFMALAVGGVLGNFLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESL- 279
           + G LQS  + + A GG++ ++  G  + S   R +F + ++L  +  +++    E+ + 
Sbjct: 254 VSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTALLPLITSAVAVLVNEQRVV 313

Query: 280 --GLLQLPEPDVHGKSIMENVRNQMSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFC 339
                Q     +     ++  +  M  L  AI+ P++F P  +I    A  P    ++F 
Sbjct: 314 RPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPTLFIFLWQA-TPHSDSAMFY 373

Query: 340 YQTQCLHLDPSIIGMSRVVSQLVLLSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIV 399
           + T  L   P  +G  ++V+ +  L    LY+ + K V ++K+  +  I      +  ++
Sbjct: 374 FTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLRKIFLVTTIFGTGLGMTQVI 433

Query: 400 LVNQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVC 459
           LV+  N +LGIS+E FA+  S +   +AQ   +P  VL A L P+G E +L +   S   
Sbjct: 434 LVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAARLCPEGMEATLFATLMSISN 493

Query: 460 LSSIVGGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLICIHFVPTISP 493
             S++GG  G GL    GI   ++ +L   I++  +++LLPL  +  +P  SP
Sbjct: 494 GGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLPLPLLGLLPRDSP 545

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018825.13.4e-299100.00putative folate-biopterin transporter 9, chloroplastic, partial [Cucurbita argyr... [more]
KAG6582427.16.1e-29696.91putative folate-biopterin transporter 9, chloroplastic, partial [Cucurbita argyr... [more]
XP_022924744.13.3e-29496.55probable folate-biopterin transporter 9, chloroplastic [Cucurbita moschata][more]
XP_023528713.11.7e-29396.37probable folate-biopterin transporter 9, chloroplastic [Cucurbita pepo subsp. pe... [more]
XP_022980264.18.2e-29395.83probable folate-biopterin transporter 9, chloroplastic isoform X1 [Cucurbita max... [more]
Match NameE-valueIdentityDescription
F4I5Q22.6e-14856.29Probable folate-biopterin transporter 8, chloroplastic OS=Arabidopsis thaliana O... [more]
O227804.9e-14760.83Probable folate-biopterin transporter 9, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9XIQ79.7e-7939.91Probable folate-biopterin transporter 7 OS=Arabidopsis thaliana OX=3702 GN=At1g6... [more]
Q8RWQ53.5e-5232.48Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g5... [more]
Q9SKZ52.2e-3026.63Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1EA281.6e-29496.55probable folate-biopterin transporter 9, chloroplastic OS=Cucurbita moschata OX=... [more]
A0A6J1IYT44.0e-29395.83probable folate-biopterin transporter 9, chloroplastic isoform X1 OS=Cucurbita m... [more]
A0A1S3AX055.1e-25685.12probable folate-biopterin transporter 9, chloroplastic OS=Cucumis melo OX=3656 G... [more]
A0A6J1DZL81.4e-25384.55probable folate-biopterin transporter 9, chloroplastic OS=Momordica charantia OX... [more]
A0A0A0L6X99.3e-25082.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G133990 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G04570.11.8e-14956.29Major facilitator superfamily protein [more]
AT2G33280.11.4e-12559.17Major facilitator superfamily protein [more]
AT1G64890.16.9e-8039.91Major facilitator superfamily protein [more]
AT5G54860.12.5e-5332.48Major facilitator superfamily protein [more]
AT2G32040.11.6e-3126.63Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004324Folate-biopterin transporterTIGRFAMTIGR00788TIGR00788coord: 174..510
e-value: 1.9E-86
score: 288.8
IPR039309Biopterin transporter familyPFAMPF03092BT1coord: 103..498
e-value: 3.0E-64
score: 217.1
IPR039309Biopterin transporter familyPANTHERPTHR31585FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTICcoord: 42..532
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 86..484
e-value: 2.7E-12
score: 48.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 93..487
NoneNo IPR availablePANTHERPTHR31585:SF12FOLATE-BIOPTERIN TRANSPORTER 9, CHLOROPLASTIC-RELATEDcoord: 42..532

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16527-RACarg16527-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane