Carg16429 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg16429 vs. NCBI nr
Match: KAG6586048.1 (Protein MULTIPOLAR SPINDLE 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020825.1 Protein MULTIPOLAR SPINDLE 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 738.8 bits (1906), Expect = 2.4e-209
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60
           MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK
Sbjct: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60

Query: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120
           QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN
Sbjct: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120

Query: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180
           DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE
Sbjct: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180

Query: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240
           ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN
Sbjct: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240

Query: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300
           TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL
Sbjct: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300

Query: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360
           IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN
Sbjct: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360

Query: 361 QVRNIQGLRELKPDILDDLVH 382
           QVRNIQGLRELKPDILDDLVH
Sbjct: 361 QVRNIQGLRELKPDILDDLVH 381

BLAST of Carg16429 vs. NCBI nr
Match: XP_022937976.1 (protein MULTIPOLAR SPINDLE 1 [Cucurbita moschata] >XP_022937977.1 protein MULTIPOLAR SPINDLE 1 [Cucurbita moschata])

HSP 1 Score: 723.8 bits (1867), Expect = 7.8e-205
Identity = 374/381 (98.16%), Postives = 377/381 (98.95%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60
           MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK
Sbjct: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60

Query: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120
           QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFD+LAKS PKRLEHDIDNRFN
Sbjct: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDNLAKSNPKRLEHDIDNRFN 120

Query: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180
           DVLRRRFLRQVR+RERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE
Sbjct: 121 DVLRRRFLRQVRYRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180

Query: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240
           ATFKIWAHQAVNFILASLKDLLP+GRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN
Sbjct: 181 ATFKIWAHQAVNFILASLKDLLPKGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240

Query: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300
           T FYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL
Sbjct: 241 TGFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300

Query: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360
           IEFLISDYLPIWSKDENFNSLLFEEWV SILHAKKALKLLESRNGLYLLYMDRVTGELAN
Sbjct: 301 IEFLISDYLPIWSKDENFNSLLFEEWVRSILHAKKALKLLESRNGLYLLYMDRVTGELAN 360

Query: 361 QVRNIQGLRELKPDILDDLVH 382
           QV NIQGLRELKPDILDDLVH
Sbjct: 361 QVSNIQGLRELKPDILDDLVH 381

BLAST of Carg16429 vs. NCBI nr
Match: XP_022969582.1 (protein MULTIPOLAR SPINDLE 1 [Cucurbita maxima])

HSP 1 Score: 723.4 bits (1866), Expect = 1.0e-204
Identity = 372/381 (97.64%), Postives = 378/381 (99.21%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60
           MSVADARSQMPVA+GGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVS+PPSDSDALRWK
Sbjct: 1   MSVADARSQMPVANGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSHPPSDSDALRWK 60

Query: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120
           QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFD+LAKS PKRLEHDIDNRFN
Sbjct: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDNLAKSNPKRLEHDIDNRFN 120

Query: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180
           DVLRRRFLRQVR+RERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE
Sbjct: 121 DVLRRRFLRQVRYRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180

Query: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240
           ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN
Sbjct: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240

Query: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300
           TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLF+MIQL
Sbjct: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFVMIQL 300

Query: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360
           IEFLI DYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNG+YLLYMDRVTGELAN
Sbjct: 301 IEFLILDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGVYLLYMDRVTGELAN 360

Query: 361 QVRNIQGLRELKPDILDDLVH 382
           QV NIQGLRELKPDILDDLVH
Sbjct: 361 QVSNIQGLRELKPDILDDLVH 381

BLAST of Carg16429 vs. NCBI nr
Match: XP_023538059.1 (protein MULTIPOLAR SPINDLE 1 [Cucurbita pepo subsp. pepo] >XP_023538060.1 protein MULTIPOLAR SPINDLE 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714.5 bits (1843), Expect = 4.8e-202
Identity = 372/381 (97.64%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60
           MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQK  QQ+QPVVSNPPSDSDALRWK
Sbjct: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQK--QQKQPVVSNPPSDSDALRWK 60

Query: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120
           QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFD+LAKS PKRLEHDIDNRFN
Sbjct: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDNLAKSNPKRLEHDIDNRFN 120

Query: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180
           DVLRRRFLRQVR+RERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE
Sbjct: 121 DVLRRRFLRQVRYRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180

Query: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240
           ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN
Sbjct: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240

Query: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300
           TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL
Sbjct: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300

Query: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360
           IEFLISDYL IWSKDENFNSLLFEEWV SILHAKKALKLLESRNGLYLLYMDRVTGELAN
Sbjct: 301 IEFLISDYLLIWSKDENFNSLLFEEWVTSILHAKKALKLLESRNGLYLLYMDRVTGELAN 360

Query: 361 QVRNIQGLRELKPDILDDLVH 382
           QV NIQGLRELKPDILDDLVH
Sbjct: 361 QVSNIQGLRELKPDILDDLVH 379

BLAST of Carg16429 vs. NCBI nr
Match: XP_038890478.1 (protein MULTIPOLAR SPINDLE 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 599.0 bits (1543), Expect = 2.9e-167
Identity = 306/382 (80.10%), Postives = 346/382 (90.58%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPP-SDSDALRW 60
           MSVA+ RSQ+P ASGGIDD+SLKLA+AISLLRSKAIQ QQ+Q  P VS+PP SDSDALRW
Sbjct: 1   MSVANGRSQIPAASGGIDDQSLKLAVAISLLRSKAIQNQQRQNPPTVSSPPSSDSDALRW 60

Query: 61  KQKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRF 120
           K+KAKERKQELIRLRE LKQAEDASHDDL SQNATCKCFFFD++ K  PKRLE   DNRF
Sbjct: 61  KRKAKERKQELIRLREVLKQAEDASHDDLLSQNATCKCFFFDNMGKLNPKRLEDGTDNRF 120

Query: 121 NDVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVG 180
           NDVLRRRFLRQVR+RER RK D+ VHRKRLLDFNE DEVE L++SVDFLVELCDT+SPVG
Sbjct: 121 NDVLRRRFLRQVRYRERGRKADNAVHRKRLLDFNEDDEVERLQASVDFLVELCDTYSPVG 180

Query: 181 EATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVF 240
           EA FK WAHQAV+FILASLKDLLP+GRN+ELIEGI++SLIMRL+R+M SP+KDE  +SV 
Sbjct: 181 EANFKNWAHQAVDFILASLKDLLPKGRNMELIEGIISSLIMRLVRKMGSPVKDELGDSVS 240

Query: 241 NTRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQ 300
           + RFYTQQL+RELGRE +IGQRA++ VSQRISAAGESLLFMDPFDDN+PSLHE LFIMIQ
Sbjct: 241 DIRFYTQQLIRELGRETYIGQRAIVYVSQRISAAGESLLFMDPFDDNYPSLHECLFIMIQ 300

Query: 301 LIEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELA 360
           LIEF+ SDYL +WSKDENF+ LLFEEW+ISILHAKKAL+LLESRNGLY+LYMDRVT ELA
Sbjct: 301 LIEFMTSDYLQMWSKDENFDRLLFEEWMISILHAKKALELLESRNGLYVLYMDRVTNELA 360

Query: 361 NQVRNIQGLRELKPDILDDLVH 382
           NQ+ NI+ LRELKPDI+DDL++
Sbjct: 361 NQISNIRRLRELKPDIVDDLIN 382

BLAST of Carg16429 vs. ExPASy Swiss-Prot
Match: F4KDF5 (Protein MULTIPOLAR SPINDLE 1 OS=Arabidopsis thaliana OX=3702 GN=MPS1 PE=2 SV=2)

HSP 1 Score: 360.9 bits (925), Expect = 1.8e-98
Identity = 196/363 (53.99%), Postives = 261/363 (71.90%), Query Frame = 0

Query: 19  DRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWKQKAKERKQELIRLREDLK 78
           + SL+LAIA+SLLRSK  Q  Q           S+SDALRWKQKAKERK+E+IRL+EDLK
Sbjct: 14  EESLRLAIAVSLLRSK-FQNHQSSSSTSRCYVSSESDALRWKQKAKERKKEIIRLQEDLK 73

Query: 79  QAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFNDVLRRRFLRQVRHRERRR 138
            AE + H DLF  NA+CKC+FFD+L     +R+    ++RFNDVLRRRFLR  R R  RR
Sbjct: 74  DAESSFHRDLFPANASCKCYFFDNLGVFSGRRIGEASESRFNDVLRRRFLRLARRRS-RR 133

Query: 139 KLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGEATFKIWAHQAVNFILASL 198
           KL     R +  + +  +E EHLR S+DFL+EL +  S   ++ F  W+HQAV+FI ASL
Sbjct: 134 KLTRSSQRLQPSEPDYEEEAEHLRISIDFLLELSEADS--NDSNFSNWSHQAVDFIFASL 193

Query: 199 KDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLK-DESENSVFNTRFYTQQLVRELGREPF 258
           K L+  GRN+E +E  ++ +I +LI RMC+P+K +E +    +  FY Q L+R+LG EPF
Sbjct: 194 KKLISMGRNLESVEESISFMITQLITRMCTPVKGNEVKQLETSVGFYVQHLIRKLGSEPF 253

Query: 259 IGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQLIEFLISDYLPIWSKDEN 318
           IGQRA+ ++SQRIS   E+LLFMDPFD++FP + E +FI+IQLIEFLI DYL  W+ +E 
Sbjct: 254 IGQRAIFAISQRISILAENLLFMDPFDESFPEMDECMFILIQLIEFLICDYLLPWA-NEA 313

Query: 319 FNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELANQVRNIQGLRELKPDILD 378
           F++++FEEW+ S++HA+KA+K LE RNGLYLLYMDRVTGELA +V  I   RE++P ILD
Sbjct: 314 FDNVMFEEWIASVVHARKAVKALEERNGLYLLYMDRVTGELAKRVGQITSFREVEPAILD 371

Query: 379 DLV 381
            ++
Sbjct: 374 KIL 371

BLAST of Carg16429 vs. ExPASy TrEMBL
Match: A0A6J1FBV6 (protein MULTIPOLAR SPINDLE 1 OS=Cucurbita moschata OX=3662 GN=LOC111444200 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.8e-205
Identity = 374/381 (98.16%), Postives = 377/381 (98.95%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60
           MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK
Sbjct: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60

Query: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120
           QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFD+LAKS PKRLEHDIDNRFN
Sbjct: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDNLAKSNPKRLEHDIDNRFN 120

Query: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180
           DVLRRRFLRQVR+RERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE
Sbjct: 121 DVLRRRFLRQVRYRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180

Query: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240
           ATFKIWAHQAVNFILASLKDLLP+GRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN
Sbjct: 181 ATFKIWAHQAVNFILASLKDLLPKGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240

Query: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300
           T FYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL
Sbjct: 241 TGFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300

Query: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360
           IEFLISDYLPIWSKDENFNSLLFEEWV SILHAKKALKLLESRNGLYLLYMDRVTGELAN
Sbjct: 301 IEFLISDYLPIWSKDENFNSLLFEEWVRSILHAKKALKLLESRNGLYLLYMDRVTGELAN 360

Query: 361 QVRNIQGLRELKPDILDDLVH 382
           QV NIQGLRELKPDILDDLVH
Sbjct: 361 QVSNIQGLRELKPDILDDLVH 381

BLAST of Carg16429 vs. ExPASy TrEMBL
Match: A0A6J1HWP8 (protein MULTIPOLAR SPINDLE 1 OS=Cucurbita maxima OX=3661 GN=LOC111468560 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.0e-205
Identity = 372/381 (97.64%), Postives = 378/381 (99.21%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60
           MSVADARSQMPVA+GGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVS+PPSDSDALRWK
Sbjct: 1   MSVADARSQMPVANGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSHPPSDSDALRWK 60

Query: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120
           QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFD+LAKS PKRLEHDIDNRFN
Sbjct: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDNLAKSNPKRLEHDIDNRFN 120

Query: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180
           DVLRRRFLRQVR+RERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE
Sbjct: 121 DVLRRRFLRQVRYRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180

Query: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240
           ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN
Sbjct: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240

Query: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300
           TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLF+MIQL
Sbjct: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFVMIQL 300

Query: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360
           IEFLI DYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNG+YLLYMDRVTGELAN
Sbjct: 301 IEFLILDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGVYLLYMDRVTGELAN 360

Query: 361 QVRNIQGLRELKPDILDDLVH 382
           QV NIQGLRELKPDILDDLVH
Sbjct: 361 QVSNIQGLRELKPDILDDLVH 381

BLAST of Carg16429 vs. ExPASy TrEMBL
Match: A0A6J1DWJ3 (protein MULTIPOLAR SPINDLE 1 OS=Momordica charantia OX=3673 GN=LOC111024716 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.9e-164
Identity = 306/381 (80.31%), Postives = 338/381 (88.71%), Query Frame = 0

Query: 1   MSVADARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWK 60
           MSVAD  SQ+P     IDD+SLKLAIAISLLRSK IQKQQ      VSNPPSDSDALRWK
Sbjct: 1   MSVADGGSQIPAP---IDDQSLKLAIAISLLRSKVIQKQQH----TVSNPPSDSDALRWK 60

Query: 61  QKAKERKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFN 120
           +KAKERKQELIRLREDLKQAED SH DLFSQNATCKCFFFD+L K  PK+LE   DNRFN
Sbjct: 61  RKAKERKQELIRLREDLKQAEDPSHGDLFSQNATCKCFFFDNLGKFNPKQLEDCTDNRFN 120

Query: 121 DVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGE 180
           DVLRRRFLRQVR+RERRRK DS ++RKRLLDFNEVDEVEHLR+SVDFLVELCDTFSPVGE
Sbjct: 121 DVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEHLRASVDFLVELCDTFSPVGE 180

Query: 181 ATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFN 240
           A FK WAHQAV+FILASLKDLLPR R++E IEGI++SLIMRLIRRMCSPLKD+S +S  +
Sbjct: 181 ANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIMRLIRRMCSPLKDDSGDSDSD 240

Query: 241 TRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQL 300
            RFYTQQL RELGREP+IGQRA+ISVSQRISA GES LFMDPFDDNFPSLHE LFIMIQL
Sbjct: 241 VRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFMDPFDDNFPSLHECLFIMIQL 300

Query: 301 IEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELAN 360
           +EFL+SDYLP+WS D+NF++LL EE VISILHAKKAL+LLESRNG+Y+LY+DRV GELAN
Sbjct: 301 VEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELLESRNGVYVLYVDRVAGELAN 360

Query: 361 QVRNIQGLRELKPDILDDLVH 382
           Q+ NI  LRELKPD+LDDL+H
Sbjct: 361 QISNIPRLRELKPDVLDDLIH 374

BLAST of Carg16429 vs. ExPASy TrEMBL
Match: A0A1S3BUG1 (uncharacterized protein LOC103493647 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493647 PE=4 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.1e-159
Identity = 293/376 (77.93%), Postives = 330/376 (87.77%), Query Frame = 0

Query: 6   ARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWKQKAKE 65
           A SQ+P ASGGIDD+SLKLAIA SLL SKAIQ Q QQ  P VS+PPS S+ALRWK+KAKE
Sbjct: 2   AESQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSSPPSHSNALRWKRKAKE 61

Query: 66  RKQELIRLREDLKQAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFNDVLRR 125
           RKQELIRLRE+LKQAEDASHDD  SQN+TCKCFFFD+L K  P R E   DNRFNDVLRR
Sbjct: 62  RKQELIRLRENLKQAEDASHDDFLSQNSTCKCFFFDNLGKLNPTRPEDGTDNRFNDVLRR 121

Query: 126 RFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGEATFKI 185
           RFLRQVR+RERRRK D+ VHRKRLLDFNE DEVE L++SVDFLVEL DT+SPVGE  FK 
Sbjct: 122 RFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVERLQASVDFLVELYDTYSPVGEVNFKN 181

Query: 186 WAHQAVNFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLKDESENSVFNTRFYT 245
           WAHQA +FILASLKDLLP+GR VELIEGI++SLIMRLIR+M SP+KDE E+S  + +FY 
Sbjct: 182 WAHQAADFILASLKDLLPKGRYVELIEGIISSLIMRLIRKMHSPVKDELEDSASDVQFYA 241

Query: 246 QQLVRELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQLIEFLI 305
           Q L+RELGRE +IGQRA++SVSQRISA GESLLFMDPFDDNFPSLHE LFIMIQLIEFLI
Sbjct: 242 QHLIRELGRETYIGQRAIVSVSQRISAVGESLLFMDPFDDNFPSLHEYLFIMIQLIEFLI 301

Query: 306 SDYLPIWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELANQVRNI 365
           SDYL +WSKD NF+SLLFEE +ISI+HAKKAL+LLESRNGLY+LYMDRVTG+LANQ+ NI
Sbjct: 302 SDYLKMWSKDRNFDSLLFEEGMISIVHAKKALELLESRNGLYILYMDRVTGDLANQISNI 361

Query: 366 QGLRELKPDILDDLVH 382
             LRE+KPDI+DDL+H
Sbjct: 362 PRLREIKPDIVDDLMH 377

BLAST of Carg16429 vs. ExPASy TrEMBL
Match: A0A1S3BUI0 (uncharacterized protein LOC103493647 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493647 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 8.0e-155
Identity = 293/403 (72.70%), Postives = 330/403 (81.89%), Query Frame = 0

Query: 6   ARSQMPVASGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWKQKAKE 65
           A SQ+P ASGGIDD+SLKLAIA SLL SKAIQ Q QQ  P VS+PPS S+ALRWK+KAKE
Sbjct: 2   AESQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSSPPSHSNALRWKRKAKE 61

Query: 66  RKQELIRLREDLKQAE---------------------------DASHDDLFSQNATCKCF 125
           RKQELIRLRE+LKQAE                           DASHDD  SQN+TCKCF
Sbjct: 62  RKQELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCF 121

Query: 126 FFDDLAKSKPKRLEHDIDNRFNDVLRRRFLRQVRHRERRRKLDSGVHRKRLLDFNEVDEV 185
           FFD+L K  P R E   DNRFNDVLRRRFLRQVR+RERRRK D+ VHRKRLLDFNE DEV
Sbjct: 122 FFDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEV 181

Query: 186 EHLRSSVDFLVELCDTFSPVGEATFKIWAHQAVNFILASLKDLLPRGRNVELIEGIVTSL 245
           E L++SVDFLVEL DT+SPVGE  FK WAHQA +FILASLKDLLP+GR VELIEGI++SL
Sbjct: 182 ERLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSL 241

Query: 246 IMRLIRRMCSPLKDESENSVFNTRFYTQQLVRELGREPFIGQRAVISVSQRISAAGESLL 305
           IMRLIR+M SP+KDE E+S  + +FY Q L+RELGRE +IGQRA++SVSQRISA GESLL
Sbjct: 242 IMRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLL 301

Query: 306 FMDPFDDNFPSLHESLFIMIQLIEFLISDYLPIWSKDENFNSLLFEEWVISILHAKKALK 365
           FMDPFDDNFPSLHE LFIMIQLIEFLISDYL +WSKD NF+SLLFEE +ISI+HAKKAL+
Sbjct: 302 FMDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALE 361

Query: 366 LLESRNGLYLLYMDRVTGELANQVRNIQGLRELKPDILDDLVH 382
           LLESRNGLY+LYMDRVTG+LANQ+ NI  LRE+KPDI+DDL+H
Sbjct: 362 LLESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of Carg16429 vs. TAIR 10
Match: AT5G57880.1 (multipolar spindle 1 )

HSP 1 Score: 356.3 bits (913), Expect = 3.1e-98
Identity = 195/370 (52.70%), Postives = 261/370 (70.54%), Query Frame = 0

Query: 19  DRSLKLAIAISLLRSKAIQKQQQQQQPVVSNPPSDSDALRWKQKAKERKQELIRLREDLK 78
           + SL+LAIA+SLLRSK  Q  Q           S+SDALRWKQKAKERK+E+IRL+EDLK
Sbjct: 14  EESLRLAIAVSLLRSK-FQNHQSSSSTSRCYVSSESDALRWKQKAKERKKEIIRLQEDLK 73

Query: 79  QAEDASHDDLFSQNATCKCFFFDDLAKSKPKRLEHDIDNRFNDVLRRRFLR-------QV 138
            AE + H DLF  NA+CKC+FFD+L     +R+    ++RFNDVLRRRFLR        +
Sbjct: 74  DAESSFHRDLFPANASCKCYFFDNLGVFSGRRIGEASESRFNDVLRRRFLRLACVVILSL 133

Query: 139 RHRERRRKLDSGVHRKRLLDFNEVDEVEHLRSSVDFLVELCDTFSPVGEATFKIWAHQAV 198
             R  RRKL     R +  + +  +E EHLR S+DFL+EL +  S   ++ F  W+HQAV
Sbjct: 134 ARRRSRRKLTRSSQRLQPSEPDYEEEAEHLRISIDFLLELSEADS--NDSNFSNWSHQAV 193

Query: 199 NFILASLKDLLPRGRNVELIEGIVTSLIMRLIRRMCSPLK-DESENSVFNTRFYTQQLVR 258
           +FI ASLK L+  GRN+E +E  ++ +I +LI RMC+P+K +E +    +  FY Q L+R
Sbjct: 194 DFIFASLKKLISMGRNLESVEESISFMITQLITRMCTPVKGNEVKQLETSVGFYVQHLIR 253

Query: 259 ELGREPFIGQRAVISVSQRISAAGESLLFMDPFDDNFPSLHESLFIMIQLIEFLISDYLP 318
           +LG EPFIGQRA+ ++SQRIS   E+LLFMDPFD++FP + E +FI+IQLIEFLI DYL 
Sbjct: 254 KLGSEPFIGQRAIFAISQRISILAENLLFMDPFDESFPEMDECMFILIQLIEFLICDYLL 313

Query: 319 IWSKDENFNSLLFEEWVISILHAKKALKLLESRNGLYLLYMDRVTGELANQVRNIQGLRE 378
            W+ +E F++++FEEW+ S++HA+KA+K LE RNGLYLLYMDRVTGELA +V  I   RE
Sbjct: 314 PWA-NEAFDNVMFEEWIASVVHARKAVKALEERNGLYLLYMDRVTGELAKRVGQITSFRE 373

Query: 379 LKPDILDDLV 381
           ++P ILD ++
Sbjct: 374 VEPAILDKIL 379