Carg16357 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16357
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	22.0 kDa heat shock protein-like
Location	Carg_Chr19: 6118465 .. 6120157 (+)
RNA-Seq Expression	Carg16357
Synteny	Carg16357

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGCGTTCTGTTCTTTGTATTTGAGAAGACGGCGATGAGTATGGGGCTACCACGACGCATGGTTTGCATCTTTCTCCCACTCGCAGTGTTGCTGTTTGTCACCCACCAACATGCCGCCCACGCTCTCATGCCCTACCGCTCCATCTGGGACATCATGCAACCAGCCATCATGCAAGGTAACTTAATATAGACGGAGTAGGTTAGGAATCACGATCCTATACAATATATCAACTCTTTAATTAGTCAAGGTGAGATTCATTCTCTAATTTTTTAACCATAGGATTCACTCTAATAATCTTCTTTTCAAAGAATGGTAAGATCTTTGAAACGCAAGGAAGAGTTGATTCGTGCGAAATTACCTGTTTTTATCTATCCGACCCATTTCGTACCCCTAATCTATGGGTACTTTGGACCGTTTTCTCTTAATTGAGTCGCTCTCAAGTAAAGTACACTTTGCTTTTAGTGTCCTTGAGTAGTCACTTCTTAATCGATGCTCGGTTCTTTGGTTTTCTTTTCTTGAGTTCTCGAATTACTTATTTTGTAGTGGCAATCTCTGAATTACCTGGATCTCGAGCCATGTGGTGTTCCAACTAGTTTCAAAAATGTACTTTAGTGTCTTTGAATAGTCTCTTCTTAATTGCTGTTCGGTTCTTTGGTTTTTTTTTTCTTGAGCTCTCAAACTACTTATTTTGGAGTGGCAACCCCTGAATTACCTGAATCTCTATGTGGTGTTCCAACCCAGTTTAAAAAAAAAATTACTTATTTTGGAGTGGCAACCCCTGAATCTCGAGCTATGTGGTGTTCCAACCTAGTTTCAAAAATGAACTTTTTGGATCGTGAAAGCGGGATTTCTTGACATTGTATATTAAAATTCATTAAAGCACGATTTGCATAAATGAAGCTCCAATAGAAAAATAGTGGAAAGAAAAAATAATTATACCTTTTTGTTCAATAAAATTGCACCTTTTCGTTCGAGAATTGAATTTAATATTCTCGGTTTTGAACATAAGCTCTTGTGATTCTTAATCGTTATTATTTCGGTGGGTGTCACAGGCGGGTTTTCGGAGGATCCATTCAGAATCCTAGAGCAGAGTCCCCTAGGCGTTCCAAGGTCAGCGGTGGACACAGTGGCAATAGCCCGCACGGACTGGAAGGAGACAAGCGGGGAGCACATGATATGGATGGACATTCCCGGCGTGAAGAAGGAGGATTTAAAGATCGAGGTGGAGGAGAACAGCGTGCTGAGAATCAGCGGAGAGATGAAGGGAGAGAGCGAGGTGGAAGGGGAGAGATGGCACCGGGCGGAGAGGAGTTCGTCGGGGAGGTTCTGGAGGCAGTTCAGGCTGCCGGGGAACGCCAACATGGAAGGGATCAAAGCCCACTTGGAGAACGGGGTGCTGAAGGTGACGGTGCCGAAGCTGCCGGAGGAGAAGAAGAAGGAGGCGAAGGTGGTGAAGATCGAGGAAGGGAGCATGTCCGGCGGCGAGGATCTGAAGGCTTCCAGAGCTCAAGTCTAAAATAATGAGACGGATGTTGAAGGATCTTATTTAATAGGTTTATGCTTTGGATGTAATTTCTATGAGAATAATAAATTTTGGAATATATATATGCGCTATTTAAAATTGTTTTATTTAACTTAAAATTAATGTGCTCTTTTTTTTAATAACAATTTCTAGATAAAGATCATATATA

mRNA sequence

AAAGCGTTCTGTTCTTTGTATTTGAGAAGACGGCGATGAGTATGGGGCTACCACGACGCATGGTTTGCATCTTTCTCCCACTCGCAGTGTTGCTGTTTGTCACCCACCAACATGCCGCCCACGCTCTCATGCCCTACCGCTCCATCTGGGACATCATGCAACCAGCCATCATGCAAGGCGGGTTTTCGGAGGATCCATTCAGAATCCTAGAGCAGAGTCCCCTAGGCGTTCCAAGGTCAGCGGTGGACACAGTGGCAATAGCCCGCACGGACTGGAAGGAGACAAGCGGGGAGCACATGATATGGATGGACATTCCCGGCGTGAAGAAGGAGGATTTAAAGATCGAGGTGGAGGAGAACAGCGTGCTGAGAATCAGCGGAGAGATGAAGGGAGAGAGCGAGGTGGAAGGGGAGAGATGGCACCGGGCGGAGAGGAGTTCGTCGGGGAGGTTCTGGAGGCAGTTCAGGCTGCCGGGGAACGCCAACATGGAAGGGATCAAAGCCCACTTGGAGAACGGGGTGCTGAAGGTGACGGTGCCGAAGCTGCCGGAGGAGAAGAAGAAGGAGGCGAAGGTGGTGAAGATCGAGGAAGGGAGCATGTCCGGCGGCGAGGATCTGAAGGCTTCCAGAGCTCAAGTCTAAAATAATGAGACGGATGTTGAAGGATCTTATTTAATAGGTTTATGCTTTGGATGTAATTTCTATGAGAATAATAAATTTTGGAATATATATATGCGCTATTTAAAATTGTTTTATTTAACTTAAAATTAATGTGCTCTTTTTTTTAATAACAATTTCTAGATAAAGATCATATATA

Coding sequence (CDS)

ATGAGTATGGGGCTACCACGACGCATGGTTTGCATCTTTCTCCCACTCGCAGTGTTGCTGTTTGTCACCCACCAACATGCCGCCCACGCTCTCATGCCCTACCGCTCCATCTGGGACATCATGCAACCAGCCATCATGCAAGGCGGGTTTTCGGAGGATCCATTCAGAATCCTAGAGCAGAGTCCCCTAGGCGTTCCAAGGTCAGCGGTGGACACAGTGGCAATAGCCCGCACGGACTGGAAGGAGACAAGCGGGGAGCACATGATATGGATGGACATTCCCGGCGTGAAGAAGGAGGATTTAAAGATCGAGGTGGAGGAGAACAGCGTGCTGAGAATCAGCGGAGAGATGAAGGGAGAGAGCGAGGTGGAAGGGGAGAGATGGCACCGGGCGGAGAGGAGTTCGTCGGGGAGGTTCTGGAGGCAGTTCAGGCTGCCGGGGAACGCCAACATGGAAGGGATCAAAGCCCACTTGGAGAACGGGGTGCTGAAGGTGACGGTGCCGAAGCTGCCGGAGGAGAAGAAGAAGGAGGCGAAGGTGGTGAAGATCGAGGAAGGGAGCATGTCCGGCGGCGAGGATCTGAAGGCTTCCAGAGCTCAAGTCTAA

Protein sequence

MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQSPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKVVKIEEGSMSGGEDLKASRAQV

Homology

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BLAST of Carg16357 vs. NCBI nr
Match: KAG7011632.1 (22.0 kDa heat shock protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 398.7 bits (1023), Expect = 3.0e-107
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ
Sbjct: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE
Sbjct: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
           SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV
Sbjct: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGEDLKASRAQV
Sbjct: 181 VKIEEGSMSGGEDLKASRAQV 201

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BLAST of Carg16357 vs. NCBI nr
Match: KAG6571944.1 (Triacylglycerol lipase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 397.5 bits (1020), Expect = 6.8e-107
Identity = 200/201 (99.50%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ
Sbjct: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE
Sbjct: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
           SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV
Sbjct: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGEDLKASRAQ+
Sbjct: 181 VKIEEGSMSGGEDLKASRAQM 201

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BLAST of Carg16357 vs. NCBI nr
Match: XP_022952788.1 (22.0 kDa heat shock protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 390.2 bits (1001), Expect = 1.1e-104
Identity = 196/201 (97.51%), Postives = 198/201 (98.51%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MSMGLPR MVC+FLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ
Sbjct: 1   MSMGLPRSMVCMFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE
Sbjct: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
           SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV
Sbjct: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGED+K SRA V
Sbjct: 181 VKIEEGSMSGGEDVKPSRADV 201

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BLAST of Carg16357 vs. NCBI nr
Match: XP_023553856.1 (22.0 kDa heat shock protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.6 bits (976), Expect = 8.6e-102
Identity = 193/201 (96.02%), Postives = 195/201 (97.01%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MSMGLPR MVC FLPLAVLLFVTHQHAAHALMPYRS+WDIMQPAIMQGGFSEDPFRILEQ
Sbjct: 3   MSMGLPRTMVCTFLPLAVLLFVTHQHAAHALMPYRSVWDIMQPAIMQGGFSEDPFRILEQ 62

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGVPRSAVDTV+IARTDWKETSGEHMIWMDIPGVKKEDLKIEVEE SVLRISGEMKGE
Sbjct: 63  SPLGVPRSAVDTVSIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEEKSVLRISGEMKGE 122

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
           SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEE KKEAKV
Sbjct: 123 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEE-KKEAKV 182

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGEDLK SRA V
Sbjct: 183 VKIEEGSMSGGEDLKPSRADV 202

[Close]

BLAST of Carg16357 vs. NCBI nr
Match: XP_022952789.1 (22.0 kDa heat shock protein-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 7.3e-101
Identity = 192/201 (95.52%), Postives = 194/201 (96.52%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MSMGLPR MVC+FLPLAVLLFVTHQHAAHALMPYRSIWDIMQP    GGFSEDPFRILEQ
Sbjct: 1   MSMGLPRSMVCMFLPLAVLLFVTHQHAAHALMPYRSIWDIMQP----GGFSEDPFRILEQ 60

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE
Sbjct: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
           SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV
Sbjct: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGED+K SRA V
Sbjct: 181 VKIEEGSMSGGEDVKPSRADV 197

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BLAST of Carg16357 vs. ExPASy Swiss-Prot
Match: Q38806 (22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.5e-35
Identity = 75/131 (57.25%), Postives = 97/131 (74.05%), Query Frame = 0


Query: 53  DPFRILEQSPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLR 112
           DPF+ILE+ PLG+ R     ++ AR DWKET+  H I +DIPG+KK+++KIEVEEN VLR
Sbjct: 48  DPFKILERIPLGLERDTSVALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGVLR 107

Query: 113 ISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPE 172
           +SGE K E E +G++WHR ER S G+FWRQF+LP N +ME +KA LENGVL + + KL  
Sbjct: 108 VSGERKREEEKKGDQWHRVER-SYGKFWRQFKLPDNVDMESVKAKLENGVLTINLTKLSP 167

Query: 173 EKKKEAKVVKI 184
           EK K  +VV I
Sbjct: 168 EKVKGPRVVNI 177

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BLAST of Carg16357 vs. ExPASy Swiss-Prot
Match: P19244 (22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.3e-33
Identity = 72/134 (53.73%), Postives = 98/134 (73.13%), Query Frame = 0


Query: 53  DPFRILEQSPLGVPRSAVD-TVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVL 112
           DPFR+LEQ P GV +     T++ AR DWKET   H+I +D+PG+KK+D+KIEVEEN VL
Sbjct: 51  DPFRVLEQIPYGVEKHEPSITLSHARVDWKETPEGHVIMVDVPGLKKDDIKIEVEENRVL 110

Query: 113 RISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLP 172
           R+SGE K E + +G+ WHR ER S G+FWRQF+LP N +++ +KA +ENGVL +T+ KL 
Sbjct: 111 RVSGERKKEEDKKGDHWHRVER-SYGKFWRQFKLPQNVDLDSVKAKMENGVLTLTLHKLS 170

Query: 173 EEKKKEAKVVKIEE 186
            +K K  ++V I E
Sbjct: 171 HDKIKGPRMVSIVE 183

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BLAST of Carg16357 vs. ExPASy Swiss-Prot
Match: P30236 (22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 8.8e-33
Identity = 72/132 (54.55%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 53  DPFRILEQSPLGVPR-SAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVL 112
           DPFR+LE  P GV +  A   ++ AR DWKET   H+I +D+PG+K+E++K+EVEEN VL
Sbjct: 43  DPFRVLEHIPFGVDKDEASMAMSPARVDWKETPEGHVIMLDVPGLKREEIKVEVEENRVL 102

Query: 113 RISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLP 172
           R+SGE K E E +G+ WHR ER S G+FWRQFRLP N +++ +KA LENGVL +T+ KL 
Sbjct: 103 RVSGERKKEEEKKGDHWHRVER-SYGKFWRQFRLPQNVDLDSVKAKLENGVLTLTLDKLS 162

Query: 173 EEKKKEAKVVKI 184
             K K  +VV I
Sbjct: 163 PGKIKGPRVVSI 173

[Close]

BLAST of Carg16357 vs. ExPASy Swiss-Prot
Match: Q7XUW5 (23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 PE=2 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 8.2e-31
Identity = 83/184 (45.11%), Postives = 114/184 (61.96%), Query Frame = 0


Query: 16  LAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGF--SEDPFRILEQSPLGVPRSAVDTV 75
           +AV+L  T   A  AL+P+         A+ + G   + DPFRILE  P G  R  V  +
Sbjct: 16  IAVVLAST--AADGALLPWFGGGGARDEAVPELGLLAAADPFRILEHVPFGFDRDDVAML 75

Query: 76  AIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGESEVE----GERWH 135
           ++AR DW+ET   H + +D+PG++KEDL++EVE+N VLRISGE + E   E    G+ WH
Sbjct: 76  SMARVDWRETGDAHEVVVDVPGMRKEDLRVEVEDNRVLRISGERRREETTEQKGGGDHWH 135

Query: 136 RAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKVVKIEEGSMS 194
           R ER S GRFWRQ RLP NA+++ I A L+NGVL V   KL  ++ K  +VV I   + +
Sbjct: 136 REER-SYGRFWRQLRLPDNADLDSIAASLDNGVLTVRFRKLAPDQIKGPRVVGI---ASA 193

[Close]

BLAST of Carg16357 vs. ExPASy Swiss-Prot
Match: Q53M11 (21.9 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP21.9 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.0e-30
Identity = 80/173 (46.24%), Postives = 117/173 (67.63%), Query Frame = 0


Query: 48  GGFSEDPFRILEQSPL----GVPRSAV----DTVAIARTDWKETSGEHMIWMDIPGVKKE 107
           G   +DPFR+LEQSPL    GV  +A       VA+AR DWKET   H++ +D+PGV++ 
Sbjct: 36  GYMLDDPFRVLEQSPLRPAGGVAAAAAAGEPAAVALARCDWKETPEAHVVTVDVPGVRRG 95

Query: 108 DLKIEVEENS-VLRISGEMK-------GESEVEGERWHRAERSSSGRFWRQFRLPGNANM 167
           D+++EV+E S VLR+SGE +        E E +G RWHRAER ++GRFWR+FR+P  A++
Sbjct: 96  DVRVEVDEASRVLRVSGERRRAGAAEEEEGERDGVRWHRAER-AAGRFWRRFRMPPGADV 155

Query: 168 EGIKAHLENGVLKVTVPKLPEEKKKEAKVVKIEEGSMSG---GEDLKASRAQV 202
             + A L++GVL VTVPK+P  + +E +VV I +G+ +G    E +KAS+A++
Sbjct: 156 GRVAARLDDGVLTVTVPKVPGHRGREPRVVAI-DGAGAGDMEAEVVKASKAEM 206

[Close]

BLAST of Carg16357 vs. ExPASy TrEMBL
Match: A0A6J1GLK2 (22.0 kDa heat shock protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455380 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.2e-105
Identity = 196/201 (97.51%), Postives = 198/201 (98.51%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MSMGLPR MVC+FLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ
Sbjct: 1   MSMGLPRSMVCMFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE
Sbjct: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
           SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV
Sbjct: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGED+K SRA V
Sbjct: 181 VKIEEGSMSGGEDVKPSRADV 201

[Close]

BLAST of Carg16357 vs. ExPASy TrEMBL
Match: A0A6J1GMQ8 (22.0 kDa heat shock protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455380 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.5e-101
Identity = 192/201 (95.52%), Postives = 194/201 (96.52%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MSMGLPR MVC+FLPLAVLLFVTHQHAAHALMPYRSIWDIMQP    GGFSEDPFRILEQ
Sbjct: 1   MSMGLPRSMVCMFLPLAVLLFVTHQHAAHALMPYRSIWDIMQP----GGFSEDPFRILEQ 60

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE
Sbjct: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
           SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV
Sbjct: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGED+K SRA V
Sbjct: 181 VKIEEGSMSGGEDVKPSRADV 197

[Close]

BLAST of Carg16357 vs. ExPASy TrEMBL
Match: A0A6J1IBD1 (22.0 kDa heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC111470955 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.2e-96
Identity = 188/201 (93.53%), Postives = 190/201 (94.53%), Query Frame = 0


Query: 1   MSMGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQ 60
           MS GL R MVCIFLPLAV LFVTHQHAAHALMPYRSIWDIMQP    GGFSEDPFRILEQ
Sbjct: 1   MSTGLHRSMVCIFLPLAVFLFVTHQHAAHALMPYRSIWDIMQP----GGFSEDPFRILEQ 60

Query: 61  SPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120
           SPLGV RSAVDTVAIARTDWKETSGEH+IWMDIPGVKKEDLKIEVEENSVLRISGEMKGE
Sbjct: 61  SPLGVSRSAVDTVAIARTDWKETSGEHVIWMDIPGVKKEDLKIEVEENSVLRISGEMKGE 120

Query: 121 SEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKV 180
            EVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEE KKEAKV
Sbjct: 121 REVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEE-KKEAKV 180

Query: 181 VKIEEGSMSGGEDLKASRAQV 202
           VKIEEGSMSGGEDLKASRA+V
Sbjct: 181 VKIEEGSMSGGEDLKASRAEV 196

[Close]

BLAST of Carg16357 vs. ExPASy TrEMBL
Match: A0A0A0K2J0 (Heat-shock protein OS=Cucumis sativus OX=3659 GN=Csa_7G072770 PE=3 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.4e-78
Identity = 156/198 (78.79%), Postives = 173/198 (87.37%), Query Frame = 0


Query: 3   MGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQSP 62
           MGLPR  + + L LA   F T Q  A+ALMPYRSIWDIMQP    GG+SEDPFRILEQSP
Sbjct: 4   MGLPRNSLFLILGLAFYFFATQQ--ANALMPYRSIWDIMQP----GGYSEDPFRILEQSP 63

Query: 63  LGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGESE 122
           L VP+SAVDT+A+AR DWKET  EH+IWMDIPG+K+EDLKIEVEEN VLRISGEMKGE+E
Sbjct: 64  LSVPKSAVDTLAVARADWKETETEHVIWMDIPGIKREDLKIEVEENRVLRISGEMKGEAE 123

Query: 123 VEGERWHRAER-SSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKVV 182
           V GERWHRAER SSSG+FWRQFRLPGNA+MEGIKAHLENGVLKV VPKLP+EKKKEAKVV
Sbjct: 124 VAGERWHRAERMSSSGKFWRQFRLPGNADMEGIKAHLENGVLKVIVPKLPQEKKKEAKVV 183

Query: 183 KIEEGSMSGGEDLKASRA 200
           KIEEG+ SGGEDLKA++A
Sbjct: 184 KIEEGAKSGGEDLKATKA 195

[Close]

BLAST of Carg16357 vs. ExPASy TrEMBL
Match: A0A5D3C8Y8 (22.0 kDa heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00880 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 9.3e-78
Identity = 154/200 (77.00%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 3   MGLPRRMVCIFLPLAVLLFVTHQHAAHALMPYRSIWDIMQPAIMQGGFSEDPFRILEQSP 62
           MGLPR  + + L LA       Q   +ALMPYRSIWDIMQP    GG+SEDPFRILEQSP
Sbjct: 4   MGLPRNSLFLILGLAFYFLAAQQ--VNALMPYRSIWDIMQP----GGYSEDPFRILEQSP 63

Query: 63  LGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLRISGEMKGESE 122
           L VP+SAVDT+A+AR DWKET  EH+IWMDIPGVK+EDLKIEVEEN VLRISGEMKGE+E
Sbjct: 64  LSVPKSAVDTLAVARADWKETEKEHVIWMDIPGVKREDLKIEVEENRVLRISGEMKGEAE 123

Query: 123 VEGERWHRAER-SSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPEEKKKEAKVV 182
           VEGERWHRAER SSSGRFWRQFR+PGNA+MEGI+AHLENGVLKV VPKLP+EKKKEAKVV
Sbjct: 124 VEGERWHRAERMSSSGRFWRQFRMPGNADMEGIRAHLENGVLKVIVPKLPQEKKKEAKVV 183

Query: 183 KIEEGSMSGGEDLKASRAQV 202
           KIEEG+ SGGEDLKA++A++
Sbjct: 184 KIEEGAKSGGEDLKATKAEM 197

[Close]

BLAST of Carg16357 vs. TAIR 10
Match: AT4G10250.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 1.8e-36
Identity = 75/131 (57.25%), Postives = 97/131 (74.05%), Query Frame = 0


Query: 53  DPFRILEQSPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLR 112
           DPF+ILE+ PLG+ R     ++ AR DWKET+  H I +DIPG+KK+++KIEVEEN VLR
Sbjct: 48  DPFKILERIPLGLERDTSVALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGVLR 107

Query: 113 ISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPE 172
           +SGE K E E +G++WHR ER S G+FWRQF+LP N +ME +KA LENGVL + + KL  
Sbjct: 108 VSGERKREEEKKGDQWHRVER-SYGKFWRQFKLPDNVDMESVKAKLENGVLTINLTKLSP 167

Query: 173 EKKKEAKVVKI 184
           EK K  +VV I
Sbjct: 168 EKVKGPRVVNI 177

[Close]

BLAST of Carg16357 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.3e-28
Identity = 67/132 (50.76%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 53  DPFRILEQSPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLR 112
           DPF+ L+      P S+   +A AR DWKET+  H+   D+PG+KKE++K+E+E++SVL+
Sbjct: 28  DPFKELQ-----FPSSSSSAIANARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLK 87

Query: 113 ISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPE 172
           ISGE   E E + + WHR ERSS G F R+FRLP N  M+ +KA +ENGVL VTVPK+ E
Sbjct: 88  ISGERHVEKEEKQDTWHRVERSSGG-FSRKFRLPENVKMDQVKASMENGVLTVTVPKV-E 147

Query: 173 EKKKEAKVVKIE 185
             KK+A+V  I+
Sbjct: 148 TNKKKAQVKSID 152

[Close]

BLAST of Carg16357 vs. TAIR 10
Match: AT1G53540.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.3e-28
Identity = 70/139 (50.36%), Postives = 96/139 (69.06%), Query Frame = 0


Query: 50  FSEDPFRILE--QSPLGVPRS-AVDTVAI--ARTDWKETSGEHMIWMDIPGVKKEDLKIE 109
           FS D F   E   +P G+  + A+D  A   A+ DW+ET   H+   D+PG++KE++K+E
Sbjct: 19  FSLDVFDPFEGFLTPSGLANAPAMDVAAFTNAKVDWRETPEAHVFKADLPGLRKEEVKVE 78

Query: 110 VEENSVLRISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLK 169
           VE+ ++L+ISGE   E+E + ++WHR ER SSG+F R+FRLP NA ME IKA +ENGVL 
Sbjct: 79  VEDGNILQISGERSNENEEKNDKWHRVER-SSGKFTRRFRLPENAKMEEIKASMENGVLS 138

Query: 170 VTVPKLPEEKKKEAKVVKI 184
           VTVPK+P EKK E K + I
Sbjct: 139 VTVPKVP-EKKPEVKSIDI 155

[Close]

BLAST of Carg16357 vs. TAIR 10
Match: AT2G29500.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-27
Identity = 67/131 (51.15%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 53  DPFRILEQSPLGVPRSAVDTVAIARTDWKETSGEHMIWMDIPGVKKEDLKIEVEENSVLR 112
           DPF+ L  S L    SA   +  AR DW+ET   H+   D+PG+KKE++K+E+EE+SVL+
Sbjct: 26  DPFKELTSSSLSRENSA---IVNARVDWRETPEAHVFKADLPGLKKEEVKVEIEEDSVLK 85

Query: 113 ISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVPKLPE 172
           ISGE   E E + + WHR ER SSG+F R+FRLP N  M+ +KA +ENGVL VTVPK  E
Sbjct: 86  ISGERHVEKEDKNDTWHRVER-SSGQFTRRFRLPENVKMDQVKAAMENGVLTVTVPK-AE 145

Query: 173 EKKKEAKVVKI 184
            KK + K ++I
Sbjct: 146 TKKADVKSIQI 151

[Close]

BLAST of Carg16357 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-27
Identity = 68/135 (50.37%), Postives = 89/135 (65.93%), Query Frame = 0


Query: 53  DPFR--ILEQSPLGVPRSAVDTVAI--ARTDWKETSGEHMIWMDIPGVKKEDLKIEVEEN 112
           DPF       S L    +A D  A   AR DWKET   H+   D+PG+KKE++K+EVE+ 
Sbjct: 25  DPFEGFFTPSSALANASTARDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDK 84

Query: 113 SVLRISGEMKGESEVEGERWHRAERSSSGRFWRQFRLPGNANMEGIKAHLENGVLKVTVP 172
           +VL+ISGE   E+E + ++WHR ER +SG+F R+FRLP NA ME +KA +ENGVL V VP
Sbjct: 85  NVLQISGERSKENEEKNDKWHRVER-ASGKFMRRFRLPENAKMEEVKATMENGVLTVVVP 144

Query: 173 KLPEEKKKEAKVVKI 184
           K P EKK + K + I
Sbjct: 145 KAP-EKKPQVKSIDI 157

The following BLAST results are available for this feature:

BLAST of Carg16357 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011632.1	3.0e-107	100.00	22.0 kDa heat shock protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6571944.1	6.8e-107	99.50	Triacylglycerol lipase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022952788.1	1.1e-104	97.51	22.0 kDa heat shock protein-like isoform X1 [Cucurbita moschata]	[more]
XP_023553856.1	8.6e-102	96.02	22.0 kDa heat shock protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022952789.1	7.3e-101	95.52	22.0 kDa heat shock protein-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg16357 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q38806	2.5e-35	57.25	22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1	[more]
P19244	2.3e-33	53.73	22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV...	[more]
P30236	8.8e-33	54.55	22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1	[more]
Q7XUW5	8.2e-31	45.11	23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 ...	[more]
Q53M11	7.0e-30	46.24	21.9 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP21.9 ...	[more]

BLAST of Carg16357 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GLK2	5.2e-105	97.51	22.0 kDa heat shock protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1GMQ8	3.5e-101	95.52	22.0 kDa heat shock protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IBD1	1.2e-96	93.53	22.0 kDa heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC111470955 PE=...	[more]
A0A0A0K2J0	1.4e-78	78.79	Heat-shock protein OS=Cucumis sativus OX=3659 GN=Csa_7G072770 PE=3 SV=1	[more]
A0A5D3C8Y8	9.3e-78	77.00	22.0 kDa heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Carg16357 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10250.1	1.8e-36	57.25	HSP20-like chaperones superfamily protein	[more]
AT1G59860.1	2.3e-28	50.76	HSP20-like chaperones superfamily protein	[more]
AT1G53540.1	2.3e-28	50.36	HSP20-like chaperones superfamily protein	[more]
AT2G29500.1	1.1e-27	51.15	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	1.9e-27	50.37	heat shock protein 18.2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 36..184 e-value: 4.3E-37 score: 129.1
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 65..180
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 79..183 e-value: 9.2E-26 score: 90.0
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 69..184 score: 25.612413
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 182..201
None	No IPR available	PANTHER	PTHR11527:SF271	22.0 KDA CLASS IV HEAT SHOCK PROTEIN-LIKE	coord: 36..188
None	No IPR available	CDD	cd06472	ACD_ScHsp26_like	coord: 77..169 e-value: 2.06376E-45 score: 143.6
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 36..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16357-RA	Carg16357-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding