Carg16285 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16285
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionELL-associated factor 1-like
LocationCarg_Chr06: 2994818 .. 2995207 (-)
RNA-Seq ExpressionCarg16285
SyntenyCarg16285
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACAGAAGAAATCGCCATTGCTATTCCGCCTGAAGAAGTCCGCGATAAAACTCACTGCGTTCCTCCGCCTCTGCGTCGCTCGTCGACAAAATTCACGACGGCTGCGATCATTCGGTTCCGACGATCGGACGGCAGGGCTACGGAGTTTCCTAGAAGATGAGAGAATAGTTGAGAATCGTTCCGTCAGAAAGCTTGAGCGTGCTTCGAGTGCAAGCTATGTAAATTTTGAAGACGATGTCGATAAGCGATCTCAGATTTTTATCGATAAATTTCTCCGTCAATTGAGGTTAGAACGACAAATTTCTTTGCAATTAAGATACTGTAGAGTCAATAGCTCTGAAACGGAATCTGAAGAGAAATCGCCGCCGTATCCGTCGATTCGATGA

mRNA sequence

ATGGCACAGAAGAAATCGCCATTGCTATTCCGCCTGAAGAAGTCCGCGATAAAACTCACTGCGTTCCTCCGCCTCTGCGTCGCTCGTCGACAAAATTCACGACGGCTGCGATCATTCGGTTCCGACGATCGGACGGCAGGGCTACGGAGTTTCCTAGAAGATGAGAGAATAGTTGAGAATCGTTCCGTCAGAAAGCTTGAGCGTGCTTCGAGTGCAAGCTATGTAAATTTTGAAGACGATGTCGATAAGCGATCTCAGATTTTTATCGATAAATTTCTCCGTCAATTGAGGTTAGAACGACAAATTTCTTTGCAATTAAGATACTGTAGAGTCAATAGCTCTGAAACGGAATCTGAAGAGAAATCGCCGCCGTATCCGTCGATTCGATGA

Coding sequence (CDS)

ATGGCACAGAAGAAATCGCCATTGCTATTCCGCCTGAAGAAGTCCGCGATAAAACTCACTGCGTTCCTCCGCCTCTGCGTCGCTCGTCGACAAAATTCACGACGGCTGCGATCATTCGGTTCCGACGATCGGACGGCAGGGCTACGGAGTTTCCTAGAAGATGAGAGAATAGTTGAGAATCGTTCCGTCAGAAAGCTTGAGCGTGCTTCGAGTGCAAGCTATGTAAATTTTGAAGACGATGTCGATAAGCGATCTCAGATTTTTATCGATAAATTTCTCCGTCAATTGAGGTTAGAACGACAAATTTCTTTGCAATTAAGATACTGTAGAGTCAATAGCTCTGAAACGGAATCTGAAGAGAAATCGCCGCCGTATCCGTCGATTCGATGA

Protein sequence

MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDERIVENRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESEEKSPPYPSIR
Homology
BLAST of Carg16285 vs. NCBI nr
Match: KAG6596691.1 (hypothetical protein SDJN03_09871, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028225.1 hypothetical protein SDJN02_09405, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.5 bits (615), Expect = 4.0e-60
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDERIVEN 60
           MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDERIVEN
Sbjct: 1   MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDERIVEN 60

Query: 61  RSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESEE 120
           RSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESEE
Sbjct: 61  RSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESEE 120

Query: 121 KSPPYPSIR 130
           KSPPYPSIR
Sbjct: 121 KSPPYPSIR 129

BLAST of Carg16285 vs. NCBI nr
Match: XP_022145292.1 (uncharacterized protein LOC111014780 [Momordica charantia])

HSP 1 Score: 162.5 bits (410), Expect = 2.4e-36
Identity = 93/130 (71.54%), Postives = 104/130 (80.00%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLR----LCVARRQNSRRLRSFGSDDRTAGLRSFLEDER 60
           MA+KKSPL+ RLKK+  K+TA LR    LC+ARR+ S RLRSF   DRT GLRSFLEDE 
Sbjct: 1   MAEKKSPLMPRLKKAVRKITALLRLNLSLCLARRRPSGRLRSFSFSDRTVGLRSFLEDEE 60

Query: 61  IVENRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSET 120
           I EN S+R+LERASS  Y N EDDVDKRSQIFID FLR+LRLERQISLQLRY R+NSS T
Sbjct: 61  IGENCSMRRLERASSLRYGNAEDDVDKRSQIFIDNFLRRLRLERQISLQLRYYRINSSGT 120

Query: 121 ESEEKSPPYP 127
           E EE+SPP P
Sbjct: 121 EDEERSPPPP 130

BLAST of Carg16285 vs. NCBI nr
Match: KAA0052985.1 (hypothetical protein E6C27_scaffold344G00960 [Cucumis melo var. makuwa] >TYK11441.1 hypothetical protein E5676_scaffold139G00970 [Cucumis melo var. makuwa])

HSP 1 Score: 144.4 bits (363), Expect = 6.6e-31
Identity = 81/125 (64.80%), Postives = 98/125 (78.40%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDE-RIVE 60
           MA+KKSP+  RLKK+ IKLTA +RLC+  R+ S+RL SF SDDR  G+R  LED+ RI E
Sbjct: 1   MAEKKSPMASRLKKALIKLTAVVRLCLVPRRRSQRLGSFDSDDRKVGIRIVLEDQSRIAE 60

Query: 61  NRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESE 120
           N   RKLERASS+ Y  +E+DVD+R++IFI+ F RQLRLERQISLQLRY RVNS ETE E
Sbjct: 61  NSFARKLERASSSRYGGYEEDVDQRAEIFIENFRRQLRLERQISLQLRYYRVNSYETEYE 120

Query: 121 EKSPP 125
           + SPP
Sbjct: 121 QISPP 125

BLAST of Carg16285 vs. NCBI nr
Match: KAG7015798.1 (hypothetical protein SDJN02_23436, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 138.7 bits (348), Expect = 3.6e-29
Identity = 78/127 (61.42%), Postives = 95/127 (74.80%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDERIVEN 60
           MA+  +P++ R+KK+  KLTAF+RLC   R  SRR+RSF SD RTAGLRS L DE IVE 
Sbjct: 1   MAENATPVVLRMKKALTKLTAFVRLCHVFRPPSRRMRSFDSDGRTAGLRSILGDESIVEK 60

Query: 61  RSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESEE 120
            SVRKLER  S+   + EDDVD+R+QIFID F RQL LERQISL+LRY  +NSSE + E 
Sbjct: 61  CSVRKLERGESSRNGSCEDDVDQRAQIFIDNFRRQLMLERQISLKLRYYGINSSEIDYEA 120

Query: 121 KSPPYPS 128
           +SPP+ S
Sbjct: 121 RSPPFSS 127

BLAST of Carg16285 vs. NCBI nr
Match: KAG6577759.1 (hypothetical protein SDJN03_25333, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 136.3 bits (342), Expect = 1.8e-28
Identity = 77/127 (60.63%), Postives = 94/127 (74.02%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDERIVEN 60
           MA+  +P++ R+KK+  KLTAF+RLC   R  SRR+RSF SD RTAGLRS L DE IVE 
Sbjct: 1   MAENATPVVLRMKKALTKLTAFVRLCHVFRPPSRRMRSFDSDGRTAGLRSILGDESIVEK 60

Query: 61  RSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESEE 120
            SVRKLER  S+   + EDDVD+R+QIFID F RQL LERQISL+L Y  +NSSE + E 
Sbjct: 61  CSVRKLERGESSRNGSCEDDVDQRAQIFIDNFRRQLMLERQISLKLMYYGINSSEIDYEA 120

Query: 121 KSPPYPS 128
           +SPP+ S
Sbjct: 121 RSPPFSS 127

BLAST of Carg16285 vs. ExPASy TrEMBL
Match: A0A6J1CW64 (uncharacterized protein LOC111014780 OS=Momordica charantia OX=3673 GN=LOC111014780 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.1e-36
Identity = 93/130 (71.54%), Postives = 104/130 (80.00%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLR----LCVARRQNSRRLRSFGSDDRTAGLRSFLEDER 60
           MA+KKSPL+ RLKK+  K+TA LR    LC+ARR+ S RLRSF   DRT GLRSFLEDE 
Sbjct: 1   MAEKKSPLMPRLKKAVRKITALLRLNLSLCLARRRPSGRLRSFSFSDRTVGLRSFLEDEE 60

Query: 61  IVENRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSET 120
           I EN S+R+LERASS  Y N EDDVDKRSQIFID FLR+LRLERQISLQLRY R+NSS T
Sbjct: 61  IGENCSMRRLERASSLRYGNAEDDVDKRSQIFIDNFLRRLRLERQISLQLRYYRINSSGT 120

Query: 121 ESEEKSPPYP 127
           E EE+SPP P
Sbjct: 121 EDEERSPPPP 130

BLAST of Carg16285 vs. ExPASy TrEMBL
Match: A0A5D3CHZ5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00970 PE=4 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.2e-31
Identity = 81/125 (64.80%), Postives = 98/125 (78.40%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGSDDRTAGLRSFLEDE-RIVE 60
           MA+KKSP+  RLKK+ IKLTA +RLC+  R+ S+RL SF SDDR  G+R  LED+ RI E
Sbjct: 1   MAEKKSPMASRLKKALIKLTAVVRLCLVPRRRSQRLGSFDSDDRKVGIRIVLEDQSRIAE 60

Query: 61  NRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETESE 120
           N   RKLERASS+ Y  +E+DVD+R++IFI+ F RQLRLERQISLQLRY RVNS ETE E
Sbjct: 61  NSFARKLERASSSRYGGYEEDVDQRAEIFIENFRRQLRLERQISLQLRYYRVNSYETEYE 120

Query: 121 EKSPP 125
           + SPP
Sbjct: 121 QISPP 125

BLAST of Carg16285 vs. ExPASy TrEMBL
Match: A0A0A0L4Z8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G004530 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.1e-28
Identity = 79/125 (63.20%), Postives = 98/125 (78.40%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRLCVARRQNSRRLRSFGS-DDRTAGLRSFLEDE-RIV 60
           MA+KKSP+  RL+K+ +KL A LRLC+  RQ SRRL SF S DD+  G+R  LED+ RI 
Sbjct: 1   MAEKKSPMASRLQKALMKLKAVLRLCLLPRQRSRRLGSFDSDDDQKVGIRIVLEDQSRIA 60

Query: 61  ENRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVNSSETES 120
           E+  VRKLERASS+ Y  +E+DVD+R++IFI+ F RQLRLERQISLQLRY RVNS ETE 
Sbjct: 61  ESSFVRKLERASSSRYGGYEEDVDQRAEIFIENFRRQLRLERQISLQLRYYRVNSYETEY 120

Query: 121 EEKSP 124
           E++SP
Sbjct: 121 EQRSP 125

BLAST of Carg16285 vs. ExPASy TrEMBL
Match: A0A1R3J730 (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_19032 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 4.4e-12
Identity = 56/125 (44.80%), Postives = 72/125 (57.60%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRL--------CVARRQNSRRLRSFGSDDRTAGLRSFL 60
           M ++   +L R KK+  KL + L L        C  RR + R   +   +DR  GL SFL
Sbjct: 1   MGREDGSILSRFKKAVKKLNSLLSLDLTKWRLSCFLRRASGRWRPTLSFNDR-LGLHSFL 60

Query: 61  EDERIVENRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVN 118
           EDE      SVR L+R    SY   +DDVDKR++IFI  F RQL LERQ+SL+LRYCR  
Sbjct: 61  EDEETNGRSSVRALQRV--RSYGAGDDDVDKRAEIFISNFRRQLWLERQVSLELRYCRTT 120

BLAST of Carg16285 vs. ExPASy TrEMBL
Match: A0A1R3IU67 (Uncharacterized protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_09786 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 9.7e-12
Identity = 55/125 (44.00%), Postives = 72/125 (57.60%), Query Frame = 0

Query: 1   MAQKKSPLLFRLKKSAIKLTAFLRL--------CVARRQNSRRLRSFGSDDRTAGLRSFL 60
           M ++   +L R KK+  KL + L L        C  RR + +   +   +DR  GL SFL
Sbjct: 1   MGREDGSILSRFKKAVKKLNSLLSLDLTKWRLSCFLRRASGKWRPTLSFNDR-LGLHSFL 60

Query: 61  EDERIVENRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQISLQLRYCRVN 118
           EDE      SVR L+R    SY   +DDVDKR++IFI  F RQL LERQ+SL+LRYCR  
Sbjct: 61  EDEETNGTSSVRALQRV--RSYGAGDDDVDKRAEIFISNFRRQLWLERQVSLELRYCRTR 120

BLAST of Carg16285 vs. TAIR 10
Match: AT5G57510.1 (unknown protein; Has 27 Blast hits to 27 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 27; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 46.2 bits (108), Expect = 2.3e-05
Identity = 27/72 (37.50%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 42  DDRTAGLRSFLEDERIVENRSVRKLERASSASYVNFEDDVDKRSQIFIDKFLRQLRLERQ 101
           +DR     +F E+E      S   L R  S    + ++D+D ++++FI  F RQL++ERQ
Sbjct: 52  NDRPGLTAAFTENEPDSTGSSRGALYRTVSYDQ-SSDEDIDNKAEMFIANFYRQLKIERQ 111

Query: 102 ISLQLRYCRVNS 114
           ISL+L+YC+ N+
Sbjct: 112 ISLELKYCQGNN 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6596691.14.0e-60100.00hypothetical protein SDJN03_09871, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022145292.12.4e-3671.54uncharacterized protein LOC111014780 [Momordica charantia][more]
KAA0052985.16.6e-3164.80hypothetical protein E6C27_scaffold344G00960 [Cucumis melo var. makuwa] >TYK1144... [more]
KAG7015798.13.6e-2961.42hypothetical protein SDJN02_23436, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6577759.11.8e-2860.63hypothetical protein SDJN03_25333, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CW641.1e-3671.54uncharacterized protein LOC111014780 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A5D3CHZ53.2e-3164.80Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0L4Z81.1e-2863.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G004530 PE=4 SV=1[more]
A0A1R3J7304.4e-1244.80Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_19032 PE=4 SV=1[more]
A0A1R3IU679.7e-1244.00Uncharacterized protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_09786 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G57510.12.3e-0537.50unknown protein; Has 27 Blast hits to 27 proteins in 9 species: Archae - 0; Bact... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008480Protein of unknown function DUF761, plantPFAMPF05553DUF761coord: 78..109
e-value: 2.1E-6
score: 27.2
NoneNo IPR availablePANTHERPTHR33098:SF64OS08G0448100 PROTEINcoord: 6..117
NoneNo IPR availablePANTHERPTHR33098COTTON FIBER (DUF761)coord: 6..117

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16285-RACarg16285-RAmRNA