Carg16256 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16256
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	strigolactone esterase D14-like
Location	Carg_Chr06: 2827314 .. 2828745 (+)
RNA-Seq Expression	Carg16256
Synteny	Carg16256

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAGGTGGATTCAACTGTGGAGGATCATCATCATCAGCAGCAGCCATTGCTGAAGCTCTTAAGGTCAATGTGTATGGCGAGGGCATGGACACGCTCGTGTTGGCGCACGGGTTCGGCTTGGATCAAAGCGCTTGGCGCTACATGATTCCTTACCTTGCTTGCTTCTTCAAAGTGGTGGTTTTTGACTTGGTTTTCTCCCCAAATGTGAAGCCTGAGGTTTATGATGAAAGGAAGTACTCAAGTTTTGAGGGATATGCCAAAGATTTGGTGTGTGTTCTTGACCATCTCCATGTTGAAAGAAGCATTTATGTTGGACATTCAATGTCTGCTATGATTGGATGTGTTGCTGCAACCTATAGGCCTCAACTCTTTCAACATCTTATCTTGTTGAATGCCTCCCCAAGGTTCTCTAAAACTCCTCCTTTTGCTTTGTTATATAATGAAACCGTTTCTAAATCTCTCGATGTAATGACCCACGTCCACCGATAGCAGGTATTGTCCTCTTTCGTATTTCCTTTCTGGGCTTTCCGTTAAGATTTTAAAACGTTCCTGCTAGTAAAAGGATTTCACACCCTTATAAAGAATGTTTTGTTCCCCTCTCCAACCGAAGCATTTTTTATAAGAGTGTGGAAAACTCTCTCTAGTAAACGCGTTTTCATGAGGGAAAGTCGAGAAGGAAAAGTCCGAAGAGGATAGTATCTCTCGACGTAACAGCTCATGCCCACCATTAATAGGTATTGTCCTCTTTCGTATTTCCCTTCTACGCTTCCCATCAAAGTTAAAATACGCGTCTGCTAGGGAAAGGTTTCCACACCCTTATAAGAAGACTCTCTCTAGCAAACGCGTTTTAAAACTTACAAGAGAAAGCCCAAAAAGGAAAAGTCCAAAGAAGACAATATCTACTAGAAACGGGCATGTTCTTTTATAAATCAATTTTTTTACGATTAGGTTATTTTACCGACATTGTTTTTACCATAGGTATCTCAACGGTGAAGGATACAATGGAGGCTTTGAAAGGCCAATAGTTGATGCCATCTTCAACCAAATGGATAAAAACTTCACAAATTGGGTAAAAAACTTTGCTCCATTTGCAGTTTCCATAAATGATCCCAAAACAATAGCAGAGTTTGAGCATTCCTTGGGAAGAATGAAGCCTGAGATTGCCCTTAGTGTTGCAAAAATGGTGTTTTTAAGTGACTTAAGACAGCTTCTACCAAAAGTAAAGACTCCCACCACCATCATTCCTACAAGAAAAGACAACATTGTCCCAAAATTTGTTGCATCATACATAAAATCAAAGGTGGGTTGCCATGCAAAAGTGAAGATTTTGAATTCTGATGGCCACTTTCCACAGCTAACAGCTTATCCTCAGCTTTTGAAAGTTTTGGCTCAAGTTCTTCACCTCAAACATAAGAATTCTACTGCTTGA

mRNA sequence

ATGGTAGGTGGATTCAACTGTGGAGGATCATCATCATCAGCAGCAGCCATTGCTGAAGCTCTTAAGGTCAATGTGTATGGCGAGGGCATGGACACGCTCGTGTTGGCGCACGGGTTCGGCTTGGATCAAAGCGCTTGGCGCTACATGATTCCTTACCTTGCTTGCTTCTTCAAAGTGGTGGTTTTTGACTTGGTTTTCTCCCCAAATGTGAAGCCTGAGGTTTATGATGAAAGGAAGTACTCAAGTTTTGAGGGATATGCCAAAGATTTGGTGTGTGTTCTTGACCATCTCCATGTTGAAAGAAGCATTTATGTTGGACATTCAATGTCTGCTATGATTGGATGTGTTGCTGCAACCTATAGGCCTCAACTCTTTCAACATCTTATCTTGTTGAATGCCTCCCCAAGGTATCTCAACGGTGAAGGATACAATGGAGGCTTTGAAAGGCCAATAGTTGATGCCATCTTCAACCAAATGGATAAAAACTTCACAAATTGGGTAAAAAACTTTGCTCCATTTGCAGTTTCCATAAATGATCCCAAAACAATAGCAGAGTTTGAGCATTCCTTGGGAAGAATGAAGCCTGAGATTGCCCTTAGTGTTGCAAAAATGGTGTTTTTAAGTGACTTAAGACAGCTTCTACCAAAAGTAAAGACTCCCACCACCATCATTCCTACAAGAAAAGACAACATTGTCCCAAAATTTGTTGCATCATACATAAAATCAAAGGTGGGTTGCCATGCAAAAGTGAAGATTTTGAATTCTGATGGCCACTTTCCACAGCTAACAGCTTATCCTCAGCTTTTGAAAGTTTTGGCTCAAGTTCTTCACCTCAAACATAAGAATTCTACTGCTTGA

Coding sequence (CDS)

ATGGTAGGTGGATTCAACTGTGGAGGATCATCATCATCAGCAGCAGCCATTGCTGAAGCTCTTAAGGTCAATGTGTATGGCGAGGGCATGGACACGCTCGTGTTGGCGCACGGGTTCGGCTTGGATCAAAGCGCTTGGCGCTACATGATTCCTTACCTTGCTTGCTTCTTCAAAGTGGTGGTTTTTGACTTGGTTTTCTCCCCAAATGTGAAGCCTGAGGTTTATGATGAAAGGAAGTACTCAAGTTTTGAGGGATATGCCAAAGATTTGGTGTGTGTTCTTGACCATCTCCATGTTGAAAGAAGCATTTATGTTGGACATTCAATGTCTGCTATGATTGGATGTGTTGCTGCAACCTATAGGCCTCAACTCTTTCAACATCTTATCTTGTTGAATGCCTCCCCAAGGTATCTCAACGGTGAAGGATACAATGGAGGCTTTGAAAGGCCAATAGTTGATGCCATCTTCAACCAAATGGATAAAAACTTCACAAATTGGGTAAAAAACTTTGCTCCATTTGCAGTTTCCATAAATGATCCCAAAACAATAGCAGAGTTTGAGCATTCCTTGGGAAGAATGAAGCCTGAGATTGCCCTTAGTGTTGCAAAAATGGTGTTTTTAAGTGACTTAAGACAGCTTCTACCAAAAGTAAAGACTCCCACCACCATCATTCCTACAAGAAAAGACAACATTGTCCCAAAATTTGTTGCATCATACATAAAATCAAAGGTGGGTTGCCATGCAAAAGTGAAGATTTTGAATTCTGATGGCCACTTTCCACAGCTAACAGCTTATCCTCAGCTTTTGAAAGTTTTGGCTCAAGTTCTTCACCTCAAACATAAGAATTCTACTGCTTGA

Protein sequence

MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKNSTA

Homology

[Close]

BLAST of Carg16256 vs. NCBI nr
Match: KAG6596658.1 (Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028196.1 Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 578.6 bits (1490), Expect = 3.0e-161
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV
Sbjct: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY
Sbjct: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP
Sbjct: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI
Sbjct: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKNSTA 286
           KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKNSTA
Sbjct: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKNSTA 285

[Close]

BLAST of Carg16256 vs. NCBI nr
Match: XP_022945470.1 (strigolactone esterase D14-like [Cucurbita moschata])

HSP 1 Score: 565.8 bits (1457), Expect = 2.0e-157
Identity = 280/285 (98.25%), Postives = 282/285 (98.95%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           MVGGFNCGG SSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV
Sbjct: 1   MVGGFNCGG-SSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY
Sbjct: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLFQHLILLNASPRYLNGEGYNGGFERP+VDAIFNQMDKNFTNWVKNFAPFAVSINDP
Sbjct: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           KTIAE EHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVA YI
Sbjct: 181 KTIAELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYI 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKNSTA 286
           KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHL+HKNSTA
Sbjct: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQHKNSTA 284

[Close]

BLAST of Carg16256 vs. NCBI nr
Match: XP_022151368.1 (strigolactone esterase D14-like [Momordica charantia])

HSP 1 Score: 456.4 bits (1173), Expect = 1.7e-124
Identity = 224/280 (80.00%), Postives = 247/280 (88.21%), Query Frame = 0


Query: 3   GGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVF 62
           G FNCG      AAI EAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVF
Sbjct: 4   GFFNCG-----EAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVF 63

Query: 63  DLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRP 122
           D +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRP
Sbjct: 64  DSIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRP 123

Query: 123 QLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKT 182
           QLF HLILLNASPRY+N +GY GGFERP +D IF QMD+NFTNWVKNFAPFAV++ND K 
Sbjct: 124 QLFTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKA 183

Query: 183 IAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKS 242
           I+EFEHSLGRMKP+IALSVAK VFLSDLR+LLPKV+TP+TII TRKDNIVPK VA Y+KS
Sbjct: 184 ISEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKS 243

Query: 243 KVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKN 283
           K+G HA VKILNS+GHFPQL AYPQ LKVL+QVL LK KN
Sbjct: 244 KMGGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKN 278

[Close]

BLAST of Carg16256 vs. NCBI nr
Match: XP_038905280.1 (strigolactone esterase D14-like [Benincasa hispida])

HSP 1 Score: 451.1 bits (1159), Expect = 7.3e-123
Identity = 224/283 (79.15%), Postives = 247/283 (87.28%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           M GGFNCG  S    A AEALKVNVYG G +TLVLAHGFG DQSAW YMIPYLACFFKVV
Sbjct: 1   MYGGFNCGAGS----AFAEALKVNVYGNGTETLVLAHGFGSDQSAWHYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVK E+YDERKYSSF+GYAKDLVCVLDHLHV+R+IYVGHSMSAM+GCVAATY
Sbjct: 61  VFDLVFSPNVKLELYDERKYSSFDGYAKDLVCVLDHLHVKRTIYVGHSMSAMVGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLF HLILLNASPRYLN EGY GGF+RP VD IF QMD NFT WVK FAPFAV +ND 
Sbjct: 121 RPQLFHHLILLNASPRYLNAEGYFGGFKRPQVDEIFKQMDNNFTKWVKEFAPFAVRVNDS 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           K +AEFEHSLGRM P+IAL+VAKMVFLSDLR+LLPKV+TPT+II T KDNIVPK VA ++
Sbjct: 181 KAMAEFEHSLGRMNPKIALNVAKMVFLSDLRKLLPKVRTPTSIILTEKDNIVPKSVAFFM 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLK-HKN 283
           KS +G ++ VKIL S+GHFPQLTAYPQLLKVL++VL LK +KN
Sbjct: 241 KSNMGGNSNVKILKSEGHFPQLTAYPQLLKVLSKVLKLKEYKN 279

[Close]

BLAST of Carg16256 vs. NCBI nr
Match: XP_008443004.1 (PREDICTED: strigolactone esterase D14-like [Cucumis melo] >KAA0043762.1 strigolactone esterase D14-like [Cucumis melo var. makuwa] >TYK25371.1 strigolactone esterase D14-like [Cucumis melo var. makuwa])

HSP 1 Score: 438.7 bits (1127), Expect = 3.8e-119
Identity = 217/279 (77.78%), Postives = 238/279 (85.30%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           M  GFNC G S    AIAEALKVNVYG G +TLVLAHGFGLDQ+ W YMIPYLACFFKVV
Sbjct: 1   MNNGFNCRGGS----AIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVK E+YDERKYSSF+GYAKDL+CVLDHLHV+R+IYVGHSMSAM+GCVAATY
Sbjct: 61  VFDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLF HLILLNASPRYLNGEGY GGFE+P VD IF +MDKNFT W K F PFAV +N+ 
Sbjct: 121 RPQLFHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNS 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           K +AE EHSLGRM P+IALSVAKMVFLSDL +LLPKVKT T+II T KDNIVPK VA +I
Sbjct: 181 KAMAEIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFI 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLK 280
           KS +G ++ V IL S GHFPQLTAYPQLLKVL +VL LK
Sbjct: 241 KSNIGGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

[Close]

BLAST of Carg16256 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.3e-61
Identity = 117/260 (45.00%), Postives = 169/260 (65.00%), Query Frame = 0


Query: 17  IAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPEVYD 76
           I EAL V V G G   L LAHGFG DQSAW  ++PY    ++VV++DLV + +V P+ +D
Sbjct: 6   ILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVNPDYFD 65

Query: 77  ERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPR 136
             +Y++ + Y  DL+ ++D L ++   YVGHS+SAMIG +A+  RP+LF  LIL+  SPR
Sbjct: 66  FNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPR 125

Query: 137 YLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTIAEFEHSLGRMKPE 196
           +LN E Y+GGFE   ++ +F+ M+ N+  WV  FAP AV  + P  + EF  +L  M+P+
Sbjct: 126 FLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPD 185

Query: 197 IALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKILNSD 256
           I+L V++ VF SDLR +L  V+ PT +I T KD  VP  VA Y++S +G    V+ L ++
Sbjct: 186 ISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTE 245

Query: 257 GHFPQLTAYPQLLKVLAQVL 277
           GH PQL+A  QL + L + L
Sbjct: 246 GHLPQLSAPAQLAQFLRRAL 265

[Close]

BLAST of Carg16256 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.2e-60
Identity = 114/249 (45.78%), Postives = 166/249 (66.67%), Query Frame = 0


Query: 17  IAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPEVYD 76
           I +A  V V G G   LV AHGFG DQSAW+ ++PY    +KV+++DLV + +V P+ +D
Sbjct: 5   ILDAFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSVNPDHFD 64

Query: 77  ERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPR 136
            R+Y++ + Y  DL+ +LD LHV R  YVGHS+SAM G +A+  RP+LF  LIL+ ASPR
Sbjct: 65  FRRYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGASPR 124

Query: 137 YLN-GEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTIAEFEHSLGRMKP 196
           +LN GE Y+GGFE+  ++ +F+ M+ N+  WV  FAP AV  + P  + EF  +L  M+P
Sbjct: 125 FLNDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFNMRP 184

Query: 197 EIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKILNS 256
           +I+L V++ VF SDLR +L  V  P  I+ T +D  VP  VA+Y+K  +G  + V+ L++
Sbjct: 185 DISLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQWLDT 244

Query: 257 DGHFPQLTA 265
           +GH P L+A
Sbjct: 245 EGHLPHLSA 253

[Close]

BLAST of Carg16256 vs. ExPASy Swiss-Prot
Match: Q10QA5 (Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 5.4e-60
Identity = 117/281 (41.64%), Postives = 174/281 (61.92%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAA-----IAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLAC 60
           MVGG   GG  S +AA     + + L V V G G   +VL+HGFG DQSAW  ++PYL  
Sbjct: 35  MVGGGGGGGGGSGSAAPSGAKLLQILNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTR 94

Query: 61  FFKVVVFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGC 120
             +VV++DLV + +V P+ +D R+Y + + Y  DL+ +LD L + R  +VGHS+SAMIG 
Sbjct: 95  DHRVVLYDLVCAGSVNPDHFDFRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGI 154

Query: 121 VAATYRPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAV 180
           +A+  RP LF  L+L+ ASPR+LN   Y+GGFE   +  +F+ M  N++ W   +AP AV
Sbjct: 155 LASIRRPDLFAKLVLIGASPRFLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAV 214

Query: 181 SINDPKTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKF 240
             + P  + EF  +L  M+P+I+L V + VF +DLR +L  V+ P  ++ T +D  VP  
Sbjct: 215 GADVPAAVQEFSRTLFNMRPDISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPAS 274

Query: 241 VASYIKSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVL 277
           VA+Y+K+ +G    V+ L ++GH P L+A   L +VL + L
Sbjct: 275 VAAYLKAHLGGRTTVEFLQTEGHLPHLSAPSLLAQVLRRAL 315

[Close]

BLAST of Carg16256 vs. ExPASy Swiss-Prot
Match: J9U5U9 (Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.2e-59
Identity = 115/260 (44.23%), Postives = 169/260 (65.00%), Query Frame = 0


Query: 17  IAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPEVYD 76
           + +AL V V G G   LVLAHGFG DQSAW  ++P+    ++VV++DLV + +V P+ +D
Sbjct: 5   LLDALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDYRVVLYDLVCAGSVNPDFFD 64

Query: 77  ERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPR 136
            R+Y++ + Y  DL+ +LD L ++   YVGHS+SAMIG +A+  RP+LF  LIL+ ASPR
Sbjct: 65  FRRYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGILASIRRPELFSKLILIGASPR 124

Query: 137 YLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTIAEFEHSLGRMKPE 196
           +LN E Y+GGFE+  ++ +F+ M+ N+  WV  FAP AV  + P  + EF  +L  M+P+
Sbjct: 125 FLNDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAVGADVPAAVREFSRTLFNMRPD 184

Query: 197 IALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKILNSD 256
           I L V++ VF SD+R +L  VK P  I  T +D+ VP  VA+Y+K+ +G    V  LN +
Sbjct: 185 ITLFVSRTVFNSDMRGVLGLVKVPCHIFQTARDHSVPASVATYLKNHLGGKNTVHWLNIE 244

Query: 257 GHFPQLTAYPQLLKVLAQVL 277
           GH P L+A   L + L + L
Sbjct: 245 GHLPHLSAPTLLAQELRRAL 264

[Close]

BLAST of Carg16256 vs. ExPASy Swiss-Prot
Match: Q9SZU7 (Probable esterase KAI2 OS=Arabidopsis thaliana OX=3702 GN=KAI2 PE=1 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 5.0e-58
Identity = 106/257 (41.25%), Postives = 168/257 (65.37%), Query Frame = 0


Query: 17  IAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPEVYD 76
           + EA  V V G G  T+VL HGFG DQS W++++P+L   ++VV++D + +    P+ +D
Sbjct: 4   VEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFD 63

Query: 77  ERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPR 136
             +YS+ EGY+ DL+ +L+ L +E  I+VGHS+SAMIG +A+  RP LF  +++++ASPR
Sbjct: 64  FDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPR 123

Query: 137 YLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSIN-DPKTIAEFEHSLGRMKP 196
           Y+N   Y GGFE+  ++ +F  +  N+  W   FAP AV  + D   + EF  +L  M+P
Sbjct: 124 YVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRP 183

Query: 197 EIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKILNS 256
           +IALSV + +F SD+RQ+LP V  P  I+ + KD  VP  V+ Y+ + +GC + V+++ S
Sbjct: 184 DIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPS 243

Query: 257 DGHFPQLTAYPQLLKVL 273
           DGH PQL++   ++ V+
Sbjct: 244 DGHLPQLSSPDSVIPVI 260

[Close]

BLAST of Carg16256 vs. ExPASy TrEMBL
Match: A0A6J1G109 (strigolactone esterase D14-like OS=Cucurbita moschata OX=3662 GN=LOC111449693 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 9.9e-158
Identity = 280/285 (98.25%), Postives = 282/285 (98.95%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           MVGGFNCGG SSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV
Sbjct: 1   MVGGFNCGG-SSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY
Sbjct: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLFQHLILLNASPRYLNGEGYNGGFERP+VDAIFNQMDKNFTNWVKNFAPFAVSINDP
Sbjct: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           KTIAE EHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVA YI
Sbjct: 181 KTIAELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYI 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKNSTA 286
           KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHL+HKNSTA
Sbjct: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQHKNSTA 284

[Close]

BLAST of Carg16256 vs. ExPASy TrEMBL
Match: A0A6J1DC02 (strigolactone esterase D14-like OS=Momordica charantia OX=3673 GN=LOC111019326 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 8.5e-125
Identity = 224/280 (80.00%), Postives = 247/280 (88.21%), Query Frame = 0


Query: 3   GGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVF 62
           G FNCG      AAI EAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVF
Sbjct: 4   GFFNCG-----EAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVF 63

Query: 63  DLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRP 122
           D +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRP
Sbjct: 64  DSIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRP 123

Query: 123 QLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKT 182
           QLF HLILLNASPRY+N +GY GGFERP +D IF QMD+NFTNWVKNFAPFAV++ND K 
Sbjct: 124 QLFTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKA 183

Query: 183 IAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKS 242
           I+EFEHSLGRMKP+IALSVAK VFLSDLR+LLPKV+TP+TII TRKDNIVPK VA Y+KS
Sbjct: 184 ISEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKS 243

Query: 243 KVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKN 283
           K+G HA VKILNS+GHFPQL AYPQ LKVL+QVL LK KN
Sbjct: 244 KMGGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKN 278

[Close]

BLAST of Carg16256 vs. ExPASy TrEMBL
Match: A0A5A7TKE6 (Strigolactone esterase D14-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005110 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.8e-119
Identity = 217/279 (77.78%), Postives = 238/279 (85.30%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           M  GFNC G S    AIAEALKVNVYG G +TLVLAHGFGLDQ+ W YMIPYLACFFKVV
Sbjct: 1   MNNGFNCRGGS----AIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVK E+YDERKYSSF+GYAKDL+CVLDHLHV+R+IYVGHSMSAM+GCVAATY
Sbjct: 61  VFDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLF HLILLNASPRYLNGEGY GGFE+P VD IF +MDKNFT W K F PFAV +N+ 
Sbjct: 121 RPQLFHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNS 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           K +AE EHSLGRM P+IALSVAKMVFLSDL +LLPKVKT T+II T KDNIVPK VA +I
Sbjct: 181 KAMAEIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFI 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLK 280
           KS +G ++ V IL S GHFPQLTAYPQLLKVL +VL LK
Sbjct: 241 KSNIGGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

[Close]

BLAST of Carg16256 vs. ExPASy TrEMBL
Match: A0A1S3B719 (strigolactone esterase D14-like OS=Cucumis melo OX=3656 GN=LOC103486734 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.8e-119
Identity = 217/279 (77.78%), Postives = 238/279 (85.30%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           M  GFNC G S    AIAEALKVNVYG G +TLVLAHGFGLDQ+ W YMIPYLACFFKVV
Sbjct: 1   MNNGFNCRGGS----AIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVK E+YDERKYSSF+GYAKDL+CVLDHLHV+R+IYVGHSMSAM+GCVAATY
Sbjct: 61  VFDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLF HLILLNASPRYLNGEGY GGFE+P VD IF +MDKNFT W K F PFAV +N+ 
Sbjct: 121 RPQLFHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNS 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           K +AE EHSLGRM P+IALSVAKMVFLSDL +LLPKVKT T+II T KDNIVPK VA +I
Sbjct: 181 KAMAEIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFI 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLK 280
           KS +G ++ V IL S GHFPQLTAYPQLLKVL +VL LK
Sbjct: 241 KSNIGGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

[Close]

BLAST of Carg16256 vs. ExPASy TrEMBL
Match: A0A0A0LB72 (Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_3G792050 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.6e-118
Identity = 211/279 (75.63%), Postives = 238/279 (85.30%), Query Frame = 0


Query: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60
           M  GFNCGG S    AIAEAL VNVYG G +TLVLAHGFGLDQ+ W YM+PYLACFFKVV
Sbjct: 1   MYNGFNCGGGS----AIAEALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVV 60

Query: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120
           VFDLVFSPNVK E+YDERKYSSF+GYAKDL+CVLDHLHV+R+IYVGHSMSAM+GCVAATY
Sbjct: 61  VFDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATY 120

Query: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180
           RPQLF HL+LLNASPRYLNGEGY GGFE+P VD +  +MDKNFT W K FAPFAV +N+ 
Sbjct: 121 RPQLFHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNS 180

Query: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240
           K + E EHSLGRM P+IAL+VAKMVFLSDL +LLPKVKT T+II T+KDNIVPK VA +I
Sbjct: 181 KAMTEVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFI 240

Query: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLK 280
           KS +G ++ V IL S GHFPQLTA+PQLLKVL +VL LK
Sbjct: 241 KSNIGGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLK 275

[Close]

BLAST of Carg16256 vs. TAIR 10
Match: AT3G03990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 9.1e-63
Identity = 117/260 (45.00%), Postives = 169/260 (65.00%), Query Frame = 0


Query: 17  IAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPEVYD 76
           I EAL V V G G   L LAHGFG DQSAW  ++PY    ++VV++DLV + +V P+ +D
Sbjct: 6   ILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVNPDYFD 65

Query: 77  ERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPR 136
             +Y++ + Y  DL+ ++D L ++   YVGHS+SAMIG +A+  RP+LF  LIL+  SPR
Sbjct: 66  FNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPR 125

Query: 137 YLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTIAEFEHSLGRMKPE 196
           +LN E Y+GGFE   ++ +F+ M+ N+  WV  FAP AV  + P  + EF  +L  M+P+
Sbjct: 126 FLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPD 185

Query: 197 IALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKILNSD 256
           I+L V++ VF SDLR +L  V+ PT +I T KD  VP  VA Y++S +G    V+ L ++
Sbjct: 186 ISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTE 245

Query: 257 GHFPQLTAYPQLLKVLAQVL 277
           GH PQL+A  QL + L + L
Sbjct: 246 GHLPQLSAPAQLAQFLRRAL 265

[Close]

BLAST of Carg16256 vs. TAIR 10
Match: AT4G37470.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 226.1 bits (575), Expect = 3.6e-59
Identity = 106/257 (41.25%), Postives = 168/257 (65.37%), Query Frame = 0


Query: 17  IAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPEVYD 76
           + EA  V V G G  T+VL HGFG DQS W++++P+L   ++VV++D + +    P+ +D
Sbjct: 4   VEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFD 63

Query: 77  ERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPR 136
             +YS+ EGY+ DL+ +L+ L +E  I+VGHS+SAMIG +A+  RP LF  +++++ASPR
Sbjct: 64  FDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPR 123

Query: 137 YLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSIN-DPKTIAEFEHSLGRMKP 196
           Y+N   Y GGFE+  ++ +F  +  N+  W   FAP AV  + D   + EF  +L  M+P
Sbjct: 124 YVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRP 183

Query: 197 EIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKILNS 256
           +IALSV + +F SD+RQ+LP V  P  I+ + KD  VP  V+ Y+ + +GC + V+++ S
Sbjct: 184 DIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPS 243

Query: 257 DGHFPQLTAYPQLLKVL 273
           DGH PQL++   ++ V+
Sbjct: 244 DGHLPQLSSPDSVIPVI 260

[Close]

BLAST of Carg16256 vs. TAIR 10
Match: AT3G24420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 2.6e-57
Identity = 110/264 (41.67%), Postives = 171/264 (64.77%), Query Frame = 0


Query: 15  AAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFDLVFSPNVKPE- 74
           + +A A+   + G G  ++VLAHGFG DQS W  +IP L+  FKV+VFD +FS  +K + 
Sbjct: 8   SGLASAMNAKIIGSGERSMVLAHGFGGDQSVWDKIIPVLSQSFKVLVFDWLFSGAIKDQT 67

Query: 75  VYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNA 134
           +YD  KY+S + ++ DL+ +++ L     ++VGHSMS +IGC A+  RP LF +L+L+ A
Sbjct: 68  LYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCAASIKRPDLFTNLLLIAA 127

Query: 135 SPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTIAEFEHSLGRM 194
           SPRY+N E Y GGFE   +D I   +  N+  W  +F+ F V   D  ++  FE SL +M
Sbjct: 128 SPRYINSEDYKGGFESKDIDTIITSIGSNYEAWAVDFSSFVVDSRDSLSVQRFEKSLKKM 187

Query: 195 KPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKSKVGCHAKVKIL 254
           KPE AL++AK+VF SD R++L +V  P  +I    D +VP  VA +++ K+   + V+I+
Sbjct: 188 KPETALALAKIVFGSDEREILGQVSVPCHVIQPGNDVVVPVSVAYFMQEKIKGKSTVEII 247

Query: 255 -NSDGHFPQLTAYPQLLKVLAQVL 277
            ++ GHFPQ+T++ +LL V+ ++L
Sbjct: 248 EDAIGHFPQMTSHLELLGVMRRLL 271

[Close]

BLAST of Carg16256 vs. TAIR 10
Match: AT5G53050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.5e-04
Identity = 24/62 (38.71%), Postives = 32/62 (51.61%), Query Frame = 0


Query: 87  AKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPRYLNGEGYNGG 146
           A D + +LDHL  +++  +GHSM AMI C  A   P+    L LLN +          GG
Sbjct: 107 ANDSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVT---------GGG 159

Query: 147 FE 149
           FE
Sbjct: 167 FE 159

[Close]

BLAST of Carg16256 vs. TAIR 10
Match: AT5G53050.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.5e-04
Identity = 24/62 (38.71%), Postives = 32/62 (51.61%), Query Frame = 0


Query: 87  AKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQLFQHLILLNASPRYLNGEGYNGG 146
           A D + +LDHL  +++  +GHSM AMI C  A   P+    L LLN +          GG
Sbjct: 107 ANDSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVT---------GGG 159

Query: 147 FE 149
           FE
Sbjct: 167 FE 159

The following BLAST results are available for this feature:

BLAST of Carg16256 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596658.1	3.0e-161	100.00	Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_022945470.1	2.0e-157	98.25	strigolactone esterase D14-like [Cucurbita moschata]	[more]
XP_022151368.1	1.7e-124	80.00	strigolactone esterase D14-like [Momordica charantia]	[more]
XP_038905280.1	7.3e-123	79.15	strigolactone esterase D14-like [Benincasa hispida]	[more]
XP_008443004.1	3.8e-119	77.78	PREDICTED: strigolactone esterase D14-like [Cucumis melo] >KAA0043762.1 strigola...	[more]

BLAST of Carg16256 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SQR3	1.3e-61	45.00	Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1	[more]
A0A109QYD3	3.2e-60	45.78	Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1	[more]
Q10QA5	5.4e-60	41.64	Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 ...	[more]
J9U5U9	1.2e-59	44.23	Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=...	[more]
Q9SZU7	5.0e-58	41.25	Probable esterase KAI2 OS=Arabidopsis thaliana OX=3702 GN=KAI2 PE=1 SV=1	[more]

BLAST of Carg16256 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G109	9.9e-158	98.25	strigolactone esterase D14-like OS=Cucurbita moschata OX=3662 GN=LOC111449693 PE...	[more]
A0A6J1DC02	8.5e-125	80.00	strigolactone esterase D14-like OS=Momordica charantia OX=3673 GN=LOC111019326 P...	[more]
A0A5A7TKE6	1.8e-119	77.78	Strigolactone esterase D14-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3B719	1.8e-119	77.78	strigolactone esterase D14-like OS=Cucumis melo OX=3656 GN=LOC103486734 PE=4 SV=...	[more]
A0A0A0LB72	2.6e-118	75.63	Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_3G79...	[more]

BLAST of Carg16256 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03990.1	9.1e-63	45.00	alpha/beta-Hydrolases superfamily protein	[more]
AT4G37470.1	3.6e-59	41.25	alpha/beta-Hydrolases superfamily protein	[more]
AT3G24420.1	2.6e-57	41.67	alpha/beta-Hydrolases superfamily protein	[more]
AT5G53050.1	2.5e-04	38.71	alpha/beta-Hydrolases superfamily protein	[more]
AT5G53050.2	2.5e-04	38.71	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 32..133 e-value: 8.4E-10 score: 38.7
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 12..277 e-value: 4.4E-58 score: 199.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 23..275
None	No IPR available	PANTHER	PTHR43039	ESTERASE-RELATED	coord: 11..278
None	No IPR available	PANTHER	PTHR43039:SF1	SIGMA FACTOR SIGB REGULATION PROTEIN RSBQ	coord: 11..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16256-RA	Carg16256-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016787	hydrolase activity