Carg16212 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16212
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	mediator of RNA polymerase II transcription subunit 27
Location	Carg_Chr06: 2530803 .. 2534783 (+)
RNA-Seq Expression	Carg16212
Synteny	Carg16212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATAAAAGGGACGGTGGAAATTCTGCAATTAGCAGTCGTCTTGCTCACACCTCGAAGGGCTTTAGGGAAAGAAATCGAAAGTAGTAAACATCGGCGGAGGGTAAATGCGACAGACGCAGCTGCCAGCGCAGGGCCTGACTGCGACGGTGAGCCCAACCACTGTTCTGCGTCACTCTTCCGGATCCGCGGAGGAGGCGCCGCCGAAGCAGGTCGCACTCGCCATGGACAGACTCGGCCATGCCGCCCGCCTCATCGCAGACATCAGGCTCGGCGCTGATCGCCTTCTTGAAGCCCTCTGCGTCACTGCACAGCCTCACCAGAGCACCAAGCCTCTCCACTTGTTCCAAAAAGAAGACGCTTCCATGCGCCAACACCTTCTTGAGCTTCGTGCTGTTGGTATTTTGCATCACTTATTTTTCTAGGGTTACGTCTATGATTTGAATGCGCTTACTTAGGGTTAATTAAAATCATTTCGGTAGCGGGATCACTCATTCGCTAACTTCCCGGACTAGTCGATTTTAGATTCAAAATGCCCCTATTGAAGAGGGGGTTTAATTTCTGCTGGACGAGATCATACAATTGAAAGAAGGGTTAAACGTTCGCAAGATTTTAATTATTTTGCTTCAAATTTTGTCAGGAAAGCAGCTTGAAGAGTCTGGTGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAATTCCTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCAGTGGTTGCTTATGCTTGGAAACGTCAATTAGCTGGCCAGGCTGGTGCATCCGCAGTTGACAGAACCAGGTTGGTTGAGACATTTCGCACATATCTATCTAGGAAAAGTAGCTGCTCAAGGGCTTGGATTGGGAAAAACTACTTAAATTTGCAAGTTCACTCGATGTTTCTTCATATTGCTTAAGGCGGTAGCGTGTTAATTTAGACTCAACTCGCTAAATTAAGTTCCCAAATTCCTTTTATAGAGATAGTAGGAAACTGGGCTTCCAGACTCGGCTTGATAGTTTTAGTAAGTTGTGGTTGATATTTAAGGAAATTCAAGCCTCTAACAGACTGGATATTGAGTTGCTTGTAGCAACTTGACTTTTCCGGGGCAATACTTTTGAAAATACGAAGAATTTGATCAAGTATCTTATATGCATGTACGTAAGTTTATTGAATGGTCAACAATAAGAACCTGATTTGTAAGATGAATAGATGCGTTATGGAAAATGATATTCACAGCTACCGTTAGGCTACGGTTGTTATTGAACATTATAAATTATTTTTATTTCTTTTGATCATTTTTGTGTCGAAACCAGATTGTAAGGGCCCCTGAATCTAATGAAGTGATCACACAGTCTCATTGTTTTTTTCACCTTCACCGGGTTTTAATTGTTTAACAAGTTCTCTGATTCAGAGAGAGGAAGTTCACGAATGATTGGCTTTAAAATGTTTTTGCTTCTATTTTACTGTAGGAGAGTTTCAGTTGCCACTAAAGTTCAATATAATAGACAAAATCTGGTAAATTTTAATCCGACTTTGATTAGTTCAGGTAAATGCTTAACTGATGCTTTACTACATTTATAGGTTAGCTCTAAAGGCCTTCACTGACCAGAAAAGACGGTTTTTCCCTCATCTTGATGAAGAAAATGGGTCGAATATGGAGCCTGCTCAAAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTTAAACAAGAGGAGCTTAATGATTCTAGTTCACTTTCAGATATTCTAACATGTATGGAAAAGGAAGTGCCAGACCTTAAGATTTTCACATATGAGCGATTGGACTGGTTGAAGCAAGCTTCAGCACTTCCCTCACAAGCAAATGAAAATTCCATAGGAGCATTGAAAGAACATAACTATCATAGTCCTAGGATGAGCCCCAGATCACTTGATGCTACTTCTACTGATAAAGCTGCTGTGATTGAGTTGATAATCCCTTCTGTCTTTAGAGCCGTAGTGTCGCTGCATCCAGTTGGTTCGGTTGAACCAGATGCTGTAGCTTTCTTTTCTCCTGATGAGGTACTCTATTTTGTGCTAAGTACTCATTGGCAAAGTTTTCTTACTCATTATTTTGTTGTACTGGCTACAAGAAAATTCTTTCAATGAGCTGTGCTATTGTCTGTGAATGCTCTGGGAGTTTCTTTTCTCCTGATGAGGTACTTTACCTGCCAGTTGCATGTCTAACGATTTGCAACCAGTTGATTTATGAAGTAGCTGGCGTGGATCAATAGTGATTTGTTGTAATGCTTTTTATGTTCTATTTGTAGCATTGTACTTTAGCAGAACTGAGAGTAAGGTAGACATGATTTTCATAGCAAATGCAATTAGACTTTCTTAGTTGTATTCCCACCTGGTTTGATTCATTGCACCTTTTCCCATTGATTTTCCTTGTATCTCAAGGACTCAAGTTGGTTACTATTGCAGTTTATTTATAAATTTTTTGTGTTGCAACAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCGAATCATCATTTATTTAGACATGTGACGGTACCTATCGCTTTGTTCATTATGTTTACGAATTCATGTTTCTGGAGTTTGCACTCTCTTAATGACGATAATTCCTTTTTCTCAATGCAGGAGCATGCTGCCACGGTGCTGCAGTACTTTATTGGAATTCAAGCTAAAGCAGCTCTACATCCTCTATTGGTAACATCGTCCATTAAATTTTTGGACATGCTTATTCTTTCTCCACTCCATTTAAAATCCTCGAAAATTTATATGTGGTATTCTTTTTTTGTAGCTTTGGATCTGCAGCTATCAAAATTTATTTTCAAAAGCTTGTAGGTGCGTGTAAGGATTTTCAGTGCTTTCGTAATTGGTTCTATATGGATTTGACATTTTAATGGTGTGTTAATCTGGTCTTTTGCAGTAAATGCGGACGGCTACTATCAGTAAACAAACAATCAGATTTACTGCTACCTCCTGCAATTCGCCCCTACAAGCAGTTTTGTACATCTAAAATCTCATCAGCTGTGCCCGTAACCTCATTGAAAGACCAGAAGTTGGATTCTGTTCAGGCTTTCCACATTGGTTGCTTCACAGAACTGTAGTCACACCTAGCTCTACAGGTACAGCTGGATAAACCACACAATTGCTCGATCCTTTTAAAAGCCTCTAGTTGATTATGAAATTAATTTATCTGGTAACAGTTGATTACGGTAGTCACACCAGTTCCTTTTAATCAACAATTGCCGCTATTCAAGTTTGCTAAGCTCACTTTTTCATGGCTCATACTACGACATTTAGTCTGTGTTTAACGGTTATTAAATAGATATCAAACATTGCAAGGGAATTTTCTCTTCTTTCACCCTCTTCTCTGATTCCTTTGTTCTGGTCCTTTGCCTTTTACTTGCCTTCTTCCAACTGAACTATGTTTTTCTTAACTTCTACTTATCACTTTCTTACTAACAATAATAGTTATAATTCCTTTGAGCACCTCATGGACTCTGATTCACATATCATACAATATCAGTATTTTCCATGAATTAGTATGCTCAGAATTAGAAGAGGACGCAGCTTTCTTGCTATATTGTACTGCTGTTGGTGATTCACTAGCCCGTGCATTATATTTCATTTCAGGTGATACACGCTCATTTGTAAATTGCTGCACTGCGTTGCAACAAGTACATTAGAGGGAGTGATATTCAGAATATTCAGAGGAGGCAATGATAATGTCAAAAGAACCATCCAATTTCTGAGGTGGTTATTTTTATGCTGCCTCAAGTGGCTTGTCATCTTGAAGGCAAGAAAATGTCATTCATTTGCATCGATTTGAGCAGTTGCTAGCTCCTTCATACGTTTGATCACACAAACATTTGTATCTTGTCCCTTCAGATTGATGTTAGAATGATAACAAGTTCGGTGTTGGGTGCACGGCCATTTAGGTAAGTGCTTCATCATTTACTTATTCTAATATTATCTTGTACT

mRNA sequence

AAAAATAAAAGGGACGGTGGAAATTCTGCAATTAGCAGTCGTCTTGCTCACACCTCGAAGGGCTTTAGGGAAAGAAATCGAAAGTAGTAAACATCGGCGGAGGGTAAATGCGACAGACGCAGCTGCCAGCGCAGGGCCTGACTGCGACGGTGAGCCCAACCACTGTTCTGCGTCACTCTTCCGGATCCGCGGAGGAGGCGCCGCCGAAGCAGGTCGCACTCGCCATGGACAGACTCGGCCATGCCGCCCGCCTCATCGCAGACATCAGGCTCGGCGCTGATCGCCTTCTTGAAGCCCTCTGCGTCACTGCACAGCCTCACCAGAGCACCAAGCCTCTCCACTTGTTCCAAAAAGAAGACGCTTCCATGCGCCAACACCTTCTTGAGCTTCGTGCTGTTGGAAAGCAGCTTGAAGAGTCTGGTGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAATTCCTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCAGTGGTTGCTTATGCTTGGAAACGTCAATTAGCTGGCCAGGCTGGTGCATCCGCAGTTGACAGAACCAGGTTAGCTCTAAAGGCCTTCACTGACCAGAAAAGACGGTTTTTCCCTCATCTTGATGAAGAAAATGGGTCGAATATGGAGCCTGCTCAAAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTTAAACAAGAGGAGCTTAATGATTCTAGTTCACTTTCAGATATTCTAACATGTATGGAAAAGGAAGTGCCAGACCTTAAGATTTTCACATATGAGCGATTGGACTGGTTGAAGCAAGCTTCAGCACTTCCCTCACAAGCAAATGAAAATTCCATAGGAGCATTGAAAGAACATAACTATCATAGTCCTAGGATGAGCCCCAGATCACTTGATGCTACTTCTACTGATAAAGCTGCTGTGATTGAGTTGATAATCCCTTCTGTCTTTAGAGCCGTAGTGTCGCTGCATCCAGTTGGTTCGGTTGAACCAGATGCTGTAGCTTTCTTTTCTCCTGATGAGAAAATTCTTTCAATGAGCTGTGCTATTGTCTGTGAATGCTCTGGGAGTTTCTTTTCTCCTGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCGAATCATCATTTATTTAGACATGTGACGGTACCTATCGCTTTGTTCATTATGTTTACGAATTCATGTTTCTGGAGTTTGCACTCTCTTAATGACGATAATTCCTTTTTCTCAATGCAGGAGCATGCTGCCACGGTGCTGCAGTACTTTATTGGAATTCAAGCTAAAGCAGCTCTACATCCTCTATTGCTTTGGATCTGCAGCTATCAAAATTTATTTTCAAAAGCTTGTAGGTGCGTTAAATGCGGACGGCTACTATCAGTAAACAAACAATCAGATTTACTGCTACCTCCTGCAATTCGCCCCTACAAGCAGTTTTGTACATCTAAAATCTCATCAGCTGTGCCCGTAACCTCATTGAAAGACCAGAAGTTGGATTCTGTTCAGGCTTTCCACATTGGTTGCTTCACAGAACTGTAGTCACACCTAGCTCTACAGGTGATACACGCTCATTTGTAAATTGCTGCACTGCGTTGCAACAAGTACATTAGAGGGAGTGATATTCAGAATATTCAGAGGAGGCAATGATAATGTCAAAAGAACCATCCAATTTCTGAGGTGGTTATTTTTATGCTGCCTCAAGTGGCTTGTCATCTTGAAGGCAAGAAAATGTCATTCATTTGCATCGATTTGAGCAGTTGCTAGCTCCTTCATACGTTTGATCACACAAACATTTGTATCTTGTCCCTTCAGATTGATGTTAGAATGATAACAAGTTCGGTGTTGGGTGCACGGCCATTTAGGTAAGTGCTTCATCATTTACTTATTCTAATATTATCTTGTACT

Coding sequence (CDS)

ATGCGACAGACGCAGCTGCCAGCGCAGGGCCTGACTGCGACGGTGAGCCCAACCACTGTTCTGCGTCACTCTTCCGGATCCGCGGAGGAGGCGCCGCCGAAGCAGGTCGCACTCGCCATGGACAGACTCGGCCATGCCGCCCGCCTCATCGCAGACATCAGGCTCGGCGCTGATCGCCTTCTTGAAGCCCTCTGCGTCACTGCACAGCCTCACCAGAGCACCAAGCCTCTCCACTTGTTCCAAAAAGAAGACGCTTCCATGCGCCAACACCTTCTTGAGCTTCGTGCTGTTGGAAAGCAGCTTGAAGAGTCTGGTGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAATTCCTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCAGTGGTTGCTTATGCTTGGAAACGTCAATTAGCTGGCCAGGCTGGTGCATCCGCAGTTGACAGAACCAGGTTAGCTCTAAAGGCCTTCACTGACCAGAAAAGACGGTTTTTCCCTCATCTTGATGAAGAAAATGGGTCGAATATGGAGCCTGCTCAAAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTTAAACAAGAGGAGCTTAATGATTCTAGTTCACTTTCAGATATTCTAACATGTATGGAAAAGGAAGTGCCAGACCTTAAGATTTTCACATATGAGCGATTGGACTGGTTGAAGCAAGCTTCAGCACTTCCCTCACAAGCAAATGAAAATTCCATAGGAGCATTGAAAGAACATAACTATCATAGTCCTAGGATGAGCCCCAGATCACTTGATGCTACTTCTACTGATAAAGCTGCTGTGATTGAGTTGATAATCCCTTCTGTCTTTAGAGCCGTAGTGTCGCTGCATCCAGTTGGTTCGGTTGAACCAGATGCTGTAGCTTTCTTTTCTCCTGATGAGAAAATTCTTTCAATGAGCTGTGCTATTGTCTGTGAATGCTCTGGGAGTTTCTTTTCTCCTGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCGAATCATCATTTATTTAGACATGTGACGGTACCTATCGCTTTGTTCATTATGTTTACGAATTCATGTTTCTGGAGTTTGCACTCTCTTAATGACGATAATTCCTTTTTCTCAATGCAGGAGCATGCTGCCACGGTGCTGCAGTACTTTATTGGAATTCAAGCTAAAGCAGCTCTACATCCTCTATTGCTTTGGATCTGCAGCTATCAAAATTTATTTTCAAAAGCTTGTAGGTGCGTTAAATGCGGACGGCTACTATCAGTAAACAAACAATCAGATTTACTGCTACCTCCTGCAATTCGCCCCTACAAGCAGTTTTGTACATCTAAAATCTCATCAGCTGTGCCCGTAACCTCATTGAAAGACCAGAAGTTGGATTCTGTTCAGGCTTTCCACATTGGTTGCTTCACAGAACTGTAG

Protein sequence

MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEPAQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQANENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVAFFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNSCFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL

Homology

[Close]

BLAST of Carg16212 vs. NCBI nr
Match: KAG7028152.1 (Mediator of RNA polymerase II transcription subunit 27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 948.3 bits (2450), Expect = 2.5e-272
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
           FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS
Sbjct: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
           CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC
Sbjct: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 478
           GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 477

[Close]

BLAST of Carg16212 vs. NCBI nr
Match: XP_022940393.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata])

HSP 1 Score: 797.7 bits (2059), Expect = 5.3e-227
Identity = 420/477 (88.05%), Postives = 421/477 (88.26%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSNHHLFRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNHHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACS--KC 420

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 478
           GRLLSVNKQSDLLLPPAIRPYKQFCTSKISS VPVTSLKDQKLDSVQAFHIGCFTEL
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSVVPVTSLKDQKLDSVQAFHIGCFTEL 423

[Close]

BLAST of Carg16212 vs. NCBI nr
Match: XP_023005694.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita maxima])

HSP 1 Score: 796.2 bits (2055), Expect = 1.6e-226
Identity = 419/477 (87.84%), Postives = 420/477 (88.05%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQAS LPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASVLPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIG LKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGTLKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSNHHLFRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNHHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACS--KC 420

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 478
           GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 423

[Close]

BLAST of Carg16212 vs. NCBI nr
Match: XP_023539656.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 792.3 bits (2045), Expect = 2.2e-225
Identity = 417/477 (87.42%), Postives = 421/477 (88.26%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+ENGSN+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDENGSNIEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           AQKKPCFSHGVTDVKQEELNDSSSLSDILTC+EKEVPDLKIFTYERLDWLKQASAL SQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCLEKEVPDLKIFTYERLDWLKQASALSSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSNHHLFRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNHHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACS--KC 420

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 478
           GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 423

[Close]

BLAST of Carg16212 vs. NCBI nr
Match: KAG6596615.1 (Mediator of RNA polymerase II transcription subunit 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 751.9 bits (1940), Expect = 3.4e-213
Identity = 397/455 (87.25%), Postives = 400/455 (87.91%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           AQKKPCFSHGVTDVK+EELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKKEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSNHHLFRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNHHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACS--KC 401

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPV 456
           GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVP+
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPL 401

[Close]

BLAST of Carg16212 vs. ExPASy Swiss-Prot
Match: Q8RWM3 (Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana OX=3702 GN=MED27 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-112
Identity = 233/447 (52.13%), Postives = 292/447 (65.32%), Query Frame = 0


Query: 27  SAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDAS 86
           S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDAS
Sbjct: 21  SESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPLQLFLREDAS 80

Query: 87  MRQHLLELRAVGKQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 146
           MRQHL +LR +GK+LEESGVL+ESL SRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA
Sbjct: 81  MRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 140

Query: 147 SAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEPAQKKPCFSHGVTDVKQEELNDSSSLS 206
           SAVDRTRLALKAFTDQKRRFFPH+D+  G  MEP+ KK   SH + +  +EE  D  +L 
Sbjct: 141 SAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGREEPVDYKTLP 200

Query: 207 DILTCMEKEVPDLKIFTYERLDWLKQASALPSQANENSIGALKEHNYHSPRMSPRSLDAT 266
           DI + +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S ++    L   
Sbjct: 201 DIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSKLR-SGLQTE 260

Query: 267 STDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVAFFSPDEKILSMSCAIVCECSGSFFSP 326
             DK AVIEL  PS+FRA+VSL P GSV+PDAVA                      FFSP
Sbjct: 261 VVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVA----------------------FFSP 320

Query: 327 DERGSHVHARGFSNHHLFRHVTVPIALFIMFTNSCFWSLHSLNDDNSFFSMQEHAATVLQ 386
           DE GS++HARGFS +H+++H+T                              EHAAT LQ
Sbjct: 321 DEGGSYLHARGFSVYHVYKHIT------------------------------EHAATALQ 380

Query: 387 YFIGIQAKAALHPLLLWICSYQNLFSKACRCVKCGRLLSVNKQSDLLLPPAIRPYKQFCT 446
           YF+G     AL+ LLLWICS++++FSK   C KCGRLL+++K+S L+LPP  R Y++   
Sbjct: 381 YFLGFGTGTALYSLLLWICSFESVFSKP--CTKCGRLLAMDKKSALILPPLHRAYQE--- 397

Query: 447 SKISSAVPVTSLKDQKLDSVQAFHIGC 474
                 +P+       LD  +A+H  C
Sbjct: 441 ------LPLA----LNLDVCEAYHSSC 397

[Close]

BLAST of Carg16212 vs. ExPASy TrEMBL
Match: A0A6J1FP59 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=3662 GN=LOC111446014 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.6e-227
Identity = 420/477 (88.05%), Postives = 421/477 (88.26%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSNHHLFRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNHHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACS--KC 420

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 478
           GRLLSVNKQSDLLLPPAIRPYKQFCTSKISS VPVTSLKDQKLDSVQAFHIGCFTEL
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSVVPVTSLKDQKLDSVQAFHIGCFTEL 423

[Close]

BLAST of Carg16212 vs. ExPASy TrEMBL
Match: A0A6J1KZZ9 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=3661 GN=LOC111498620 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 7.5e-227
Identity = 419/477 (87.84%), Postives = 420/477 (88.05%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQSTKPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQAS LPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASVLPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIG LKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NENSIGTLKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSNHHLFRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNHHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACS--KC 420

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 478
           GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTEL 423

[Close]

BLAST of Carg16212 vs. ExPASy TrEMBL
Match: A0A6J1DC62 (mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX=3673 GN=LOC111019372 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 5.4e-201
Identity = 372/476 (78.15%), Postives = 394/476 (82.77%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MRQTQ PAQG TATVSP T   HS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTA PHQSTKPLHLFQKED SMRQHL +LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+E+  N+EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVT++KQ+ELN+  +LSDILTCMEKEVPDLKIFTYERLDWLKQA AL SQA
Sbjct: 181 ALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           N+NSIGALKEHNYHSPRM P SL A+S DK +VIELIIPSVFRAVVSLHPVGSVEPDAVA
Sbjct: 241 NDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FF+PDERGS+VHARGFS HHLFRHVT            
Sbjct: 301 ----------------------FFAPDERGSYVHARGFSVHHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYF+GIQ KAAL+PLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACS--KC 420

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTE 477
           GRLLSVNKQSDLLLPPAIRPYKQFCTS++ SAVP++SLKDQ+ D VQAFHIGCF+E
Sbjct: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHIGCFSE 422

[Close]

BLAST of Carg16212 vs. ExPASy TrEMBL
Match: A0A0A0LGT1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.3e-199
Identity = 374/476 (78.57%), Postives = 393/476 (82.56%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSN+HLFRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNYHLFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYF+G Q KAAL+PLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACS--KC 419

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTE 477
           GR LSVNKQSDLLLPPA+RPYKQFC SK SS VP+TSLKDQKLDSVQAFHI CF+E
Sbjct: 421 GRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITSLKDQKLDSVQAFHIDCFSE 419

[Close]

BLAST of Carg16212 vs. ExPASy TrEMBL
Match: A0A1S3B7L6 (mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 GN=LOC103486676 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.3e-199
Identity = 372/476 (78.15%), Postives = 393/476 (82.56%), Query Frame = 0


Query: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP T   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRHIQQPSQGQTATVSPNT---HSPSSADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLELRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLL+LRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180

Query: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+C+EKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCVEKEVPELKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300
           NENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHP+GSVEPDA+A
Sbjct: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPIGSVEPDALA 300

Query: 301 FFSPDEKILSMSCAIVCECSGSFFSPDERGSHVHARGFSNHHLFRHVTVPIALFIMFTNS 360
                                 FFSPDERGSHVHARGFSN+H+FRHVT            
Sbjct: 301 ----------------------FFSPDERGSHVHARGFSNYHVFRHVT------------ 360

Query: 361 CFWSLHSLNDDNSFFSMQEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFSKACRCVKC 420
                             EHAATVLQYF+G Q KAAL+PLLLWICSYQNLFSKAC   KC
Sbjct: 361 ------------------EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACS--KC 419

Query: 421 GRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVPVTSLKDQKLDSVQAFHIGCFTE 477
           GR LSVNKQSDLLLPP +RPYKQFC SK SSAVP+TSLKDQKLDSVQAFHI CF+E
Sbjct: 421 GRRLSVNKQSDLLLPPVVRPYKQFCASKNSSAVPITSLKDQKLDSVQAFHIDCFSE 419

[Close]

BLAST of Carg16212 vs. TAIR 10
Match: AT3G09180.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627); Has 112 Blast hits to 112 proteins in 38 species: Archae - 0; Bacteria - 0; Metazoa - 79; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 408.3 bits (1048), Expect = 8.5e-114
Identity = 233/447 (52.13%), Postives = 292/447 (65.32%), Query Frame = 0


Query: 27  SAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDAS 86
           S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDAS
Sbjct: 21  SESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPLQLFLREDAS 80

Query: 87  MRQHLLELRAVGKQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 146
           MRQHL +LR +GK+LEESGVL+ESL SRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA
Sbjct: 81  MRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 140

Query: 147 SAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEPAQKKPCFSHGVTDVKQEELNDSSSLS 206
           SAVDRTRLALKAFTDQKRRFFPH+D+  G  MEP+ KK   SH + +  +EE  D  +L 
Sbjct: 141 SAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGREEPVDYKTLP 200

Query: 207 DILTCMEKEVPDLKIFTYERLDWLKQASALPSQANENSIGALKEHNYHSPRMSPRSLDAT 266
           DI + +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S ++    L   
Sbjct: 201 DIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSKLR-SGLQTE 260

Query: 267 STDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVAFFSPDEKILSMSCAIVCECSGSFFSP 326
             DK AVIEL  PS+FRA+VSL P GSV+PDAVA                      FFSP
Sbjct: 261 VVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVA----------------------FFSP 320

Query: 327 DERGSHVHARGFSNHHLFRHVTVPIALFIMFTNSCFWSLHSLNDDNSFFSMQEHAATVLQ 386
           DE GS++HARGFS +H+++H+T                              EHAAT LQ
Sbjct: 321 DEGGSYLHARGFSVYHVYKHIT------------------------------EHAATALQ 380

Query: 387 YFIGIQAKAALHPLLLWICSYQNLFSKACRCVKCGRLLSVNKQSDLLLPPAIRPYKQFCT 446
           YF+G     AL+ LLLWICS++++FSK   C KCGRLL+++K+S L+LPP  R Y++   
Sbjct: 381 YFLGFGTGTALYSLLLWICSFESVFSKP--CTKCGRLLAMDKKSALILPPLHRAYQE--- 397

Query: 447 SKISSAVPVTSLKDQKLDSVQAFHIGC 474
                 +P+       LD  +A+H  C
Sbjct: 441 ------LPLA----LNLDVCEAYHSSC 397

The following BLAST results are available for this feature:

BLAST of Carg16212 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028152.1	2.5e-272	100.00	Mediator of RNA polymerase II transcription subunit 27 [Cucurbita argyrosperma s...	[more]
XP_022940393.1	5.3e-227	88.05	mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata]	[more]
XP_023005694.1	1.6e-226	87.84	mediator of RNA polymerase II transcription subunit 27 [Cucurbita maxima]	[more]
XP_023539656.1	2.2e-225	87.42	mediator of RNA polymerase II transcription subunit 27 [Cucurbita pepo subsp. pe...	[more]
KAG6596615.1	3.4e-213	87.25	Mediator of RNA polymerase II transcription subunit 27, partial [Cucurbita argyr...	[more]

BLAST of Carg16212 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWM3	1.2e-112	52.13	Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana O...	[more]

BLAST of Carg16212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FP59	2.6e-227	88.05	mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=...	[more]
A0A6J1KZZ9	7.5e-227	87.84	mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=36...	[more]
A0A6J1DC62	5.4e-201	78.15	mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX...	[more]
A0A0A0LGT1	1.3e-199	78.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1	[more]
A0A1S3B7L6	2.3e-199	78.15	mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 G...	[more]

BLAST of Carg16212 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09180.1	8.5e-114	52.13	CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627);...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021627	Mediator complex, subunit Med27	PFAM	PF11571	Med27	coord: 371..473 e-value: 3.5E-16 score: 59.3
IPR021627	Mediator complex, subunit Med27	PANTHER	PTHR13130	34 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATED	coord: 318..349
IPR021627	Mediator complex, subunit Med27	PANTHER	PTHR13130	34 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATED	coord: 374..445 coord: 25..307
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..32
None	No IPR available	PANTHER	PTHR13130:SF5	BNAC03G36240D PROTEIN	coord: 318..349 coord: 25..307
None	No IPR available	PANTHER	PTHR13130:SF5	BNAC03G36240D PROTEIN	coord: 374..445

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16212-RA	Carg16212-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003713	transcription coactivator activity