Carg16203 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16203
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Eukaryotic aspartyl protease family protein
Location	Carg_Chr06: 2490268 .. 2491641 (+)
RNA-Seq Expression	Carg16203
Synteny	Carg16203

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCTCCTCCCCCTCTCTGTTTCTCATACATTCTCCTCCTCTTCTCCGTTTCGGCCATTGTCGACGCCAACGCCAACTCCATCACTCTCCCTCTCTCCGCCTTCCCCCACCCTTCTTCCTCAGATCCACTCCAAAATCTCAATTTCCTCGCCTCTGCTTCCCAGAACAGAGCCCATCAAATCAAAACCCCAAAATCCAACTCCGTTTCCAAATCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCTGCTCCACTCAGCTTCGGTACCCCACCCCAGACTCTCCATTTGATATTTGATACAGGTAGCAGCCTCGTTTGGTTTCCTTGTACTTCCAAATATCTCTGTTCCCAATGTTCGTTTCCCAAAATAGATCCTCTCCGGATCCCCAGATTTGTCCCCAAATTGTCCTCTTCCTCCAAGCTTGTCGGCTGCCAGAATCCAAAATGTGCTTGGGTTTTCGGCCCTGACGTGAAGTCTCAGTGCCGGAATTGTAACCCGAAAACAGAGAACTGTACCCAAACATGTCCTGCTTATGCTGTTCAGTATGGCTCTGGTTCCACGGCCGGGCTTTTGCTATCGGAGACGCTGGATTTTCCCGATCAAAAAATCCCCAATTTCGTTGTGGGGTGTTCGTTTCTTTCGATTCATCAGCCCTCTGGAATCGCTGGATTCGGCCGAGGATCCGAATCGCTTCCGTCGCAAATGGGTCTGAAGAAATTTGCCTACTGTCTCGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCGAGCTGATTCTAGATTCCGGCGGGGCAAAGACCGGCGATCTCACCTACACGCCGTTCCGCCAGAATCCCTCTGTATCTAACCATGCTTATAAGGAATACTATTACTTATCAATAAGGAAAATCCTCGTCGGTAACCAGGACGTGAAGGTGCCGTACAAGTATCTAGTGCCGGGATCCGACGGCAGCGGTGGATCTATCATTGATTCCGGCTCTACCTTCACGTTTATGGACAAACCAGTGTTCGAAGCAGTGGCGGAAGCGTTCGAGAAGCAGTTAGCGAATCGGACGAGAGCCACCGACGTGGAATCTGCCACCGGATTACGGCCATGTTTTGACATTTCGAAGGAGAAGTCGGTGGAGTTTCCGGAGTTGATATTCCAGTTTAAAGGCGGAGCGAAATGGGCACTGCCATTGAATAACTACTTCGCTTTGGTTAGTAGCTCCGGCGTTGCGTGTTTGACAGTTGTAACGCATAAGGAAGCGTCGGGCGGCGGCAGTGGGCCGTCCGTGATTTTGGGGGCTTTTCAACAGCAAAATTTCTATGTGGAATACGATTTGGTGAATGAAAGATTAGGATTTCGGCAACAGAGTTGCAGTTAG

mRNA sequence

ATGGCGGCTCCTCCCCCTCTCTGTTTCTCATACATTCTCCTCCTCTTCTCCGTTTCGGCCATTGTCGACGCCAACGCCAACTCCATCACTCTCCCTCTCTCCGCCTTCCCCCACCCTTCTTCCTCAGATCCACTCCAAAATCTCAATTTCCTCGCCTCTGCTTCCCAGAACAGAGCCCATCAAATCAAAACCCCAAAATCCAACTCCGTTTCCAAATCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCTGCTCCACTCAGCTTCGGTACCCCACCCCAGACTCTCCATTTGATATTTGATACAGGTAGCAGCCTCGTTTGGTTTCCTTGTACTTCCAAATATCTCTGTTCCCAATGTTCGTTTCCCAAAATAGATCCTCTCCGGATCCCCAGATTTGTCCCCAAATTGTCCTCTTCCTCCAAGCTTGTCGGCTGCCAGAATCCAAAATGTGCTTGGGTTTTCGGCCCTGACGTGAAGTCTCAGTGCCGGAATTGTAACCCGAAAACAGAGAACTGTACCCAAACATGTCCTGCTTATGCTGTTCAGTATGGCTCTGGTTCCACGGCCGGGCTTTTGCTATCGGAGACGCTGGATTTTCCCGATCAAAAAATCCCCAATTTCGTTGTGGGGTGTTCGTTTCTTTCGATTCATCAGCCCTCTGGAATCGCTGGATTCGGCCGAGGATCCGAATCGCTTCCGTCGCAAATGGGTCTGAAGAAATTTGCCTACTGTCTCGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCGAGCTGATTCTAGATTCCGGCGGGGCAAAGACCGGCGATCTCACCTACACGCCGTTCCGCCAGAATCCCTCTGTATCTAACCATGCTTATAAGGAATACTATTACTTATCAATAAGGAAAATCCTCGTCGGTAACCAGGACGTGAAGGTGCCGTACAAGTATCTAGTGCCGGGATCCGACGGCAGCGGTGGATCTATCATTGATTCCGGCTCTACCTTCACGTTTATGGACAAACCAGTGTTCGAAGCAGTGGCGGAAGCGTTCGAGAAGCAGTTAGCGAATCGGACGAGAGCCACCGACGTGGAATCTGCCACCGGATTACGGCCATGTTTTGACATTTCGAAGGAGAAGTCGGTGGAGTTTCCGGAGTTGATATTCCAGTTTAAAGGCGGAGCGAAATGGGCACTGCCATTGAATAACTACTTCGCTTTGGTTAGTAGCTCCGGCGTTGCGTGTTTGACAGTTGTAACGCATAAGGAAGCGTCGGGCGGCGGCAGTGGGCCGTCCGTGATTTTGGGGGCTTTTCAACAGCAAAATTTCTATGTGGAATACGATTTGGTGAATGAAAGATTAGGATTTCGGCAACAGAGTTGCAGTTAG

Coding sequence (CDS)

ATGGCGGCTCCTCCCCCTCTCTGTTTCTCATACATTCTCCTCCTCTTCTCCGTTTCGGCCATTGTCGACGCCAACGCCAACTCCATCACTCTCCCTCTCTCCGCCTTCCCCCACCCTTCTTCCTCAGATCCACTCCAAAATCTCAATTTCCTCGCCTCTGCTTCCCAGAACAGAGCCCATCAAATCAAAACCCCAAAATCCAACTCCGTTTCCAAATCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCTGCTCCACTCAGCTTCGGTACCCCACCCCAGACTCTCCATTTGATATTTGATACAGGTAGCAGCCTCGTTTGGTTTCCTTGTACTTCCAAATATCTCTGTTCCCAATGTTCGTTTCCCAAAATAGATCCTCTCCGGATCCCCAGATTTGTCCCCAAATTGTCCTCTTCCTCCAAGCTTGTCGGCTGCCAGAATCCAAAATGTGCTTGGGTTTTCGGCCCTGACGTGAAGTCTCAGTGCCGGAATTGTAACCCGAAAACAGAGAACTGTACCCAAACATGTCCTGCTTATGCTGTTCAGTATGGCTCTGGTTCCACGGCCGGGCTTTTGCTATCGGAGACGCTGGATTTTCCCGATCAAAAAATCCCCAATTTCGTTGTGGGGTGTTCGTTTCTTTCGATTCATCAGCCCTCTGGAATCGCTGGATTCGGCCGAGGATCCGAATCGCTTCCGTCGCAAATGGGTCTGAAGAAATTTGCCTACTGTCTCGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCGAGCTGATTCTAGATTCCGGCGGGGCAAAGACCGGCGATCTCACCTACACGCCGTTCCGCCAGAATCCCTCTGTATCTAACCATGCTTATAAGGAATACTATTACTTATCAATAAGGAAAATCCTCGTCGGTAACCAGGACGTGAAGGTGCCGTACAAGTATCTAGTGCCGGGATCCGACGGCAGCGGTGGATCTATCATTGATTCCGGCTCTACCTTCACGTTTATGGACAAACCAGTGTTCGAAGCAGTGGCGGAAGCGTTCGAGAAGCAGTTAGCGAATCGGACGAGAGCCACCGACGTGGAATCTGCCACCGGATTACGGCCATGTTTTGACATTTCGAAGGAGAAGTCGGTGGAGTTTCCGGAGTTGATATTCCAGTTTAAAGGCGGAGCGAAATGGGCACTGCCATTGAATAACTACTTCGCTTTGGTTAGTAGCTCCGGCGTTGCGTGTTTGACAGTTGTAACGCATAAGGAAGCGTCGGGCGGCGGCAGTGGGCCGTCCGTGATTTTGGGGGCTTTTCAACAGCAAAATTTCTATGTGGAATACGATTTGGTGAATGAAAGATTAGGATTTCGGCAACAGAGTTGCAGTTAG

Protein sequence

MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEASGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS

Homology

[Close]

BLAST of Carg16203 vs. NCBI nr
Match: KAG7028143.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 930.6 bits (2404), Expect = 5.1e-267
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

[Close]

BLAST of Carg16203 vs. NCBI nr
Match: XP_022940517.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])

HSP 1 Score: 927.2 bits (2395), Expect = 5.6e-266
Identity = 455/457 (99.56%), Postives = 456/457 (99.78%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSA PHPSSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEA+
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

[Close]

BLAST of Carg16203 vs. NCBI nr
Match: KAG6596604.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 926.8 bits (2394), Expect = 7.3e-266
Identity = 455/457 (99.56%), Postives = 455/457 (99.56%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSA PHPSSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAIPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQL NRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLVNRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

[Close]

BLAST of Carg16203 vs. NCBI nr
Match: XP_023540611.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 917.5 bits (2370), Expect = 4.4e-263
Identity = 450/457 (98.47%), Postives = 453/457 (99.12%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVDAN NSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANTNSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNLKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           SATGL PCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHK+A+
Sbjct: 361 SATGLLPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKDAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQ+CS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 457

[Close]

BLAST of Carg16203 vs. NCBI nr
Match: XP_023005632.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 906.7 bits (2342), Expect = 7.8e-260
Identity = 446/457 (97.59%), Postives = 448/457 (98.03%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVD NANSITLPLSAFPH SSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKS LSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKC WVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGG KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTHKEA+
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQ+CS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 457

[Close]

BLAST of Carg16203 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.9e-49
Identity = 147/489 (30.06%), Postives = 220/489 (44.99%), Query Frame = 0


Query: 16  FSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSPL 75
           FSVS++       ++  LS   H  SS PL  L   +S S  R  +    +       P+
Sbjct: 20  FSVSSLSTPLLLHLSHSLSTSKH--SSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPI 79

Query: 76  SPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVP 135
           S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C    + P        
Sbjct: 80  S--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTCILCESKPLPPSP----PS 139

Query: 136 KLSSSSKLVGCQNPKCAWVFGPDVKSQ---CRNCN---PKTENCTQT---CPAYAVQYGS 195
            LSSS+  V C +P C+        S      NC     +T +C  +   CP +   YG 
Sbjct: 140 SLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 199

Query: 196 GSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGL------K 255
           GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP+Q+ +       
Sbjct: 200 GSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGN 259

Query: 256 KFAYCLASRKFDDS--PHSGELIL--------------------DSGGAKTGDLTYTPFR 315
            F+YCL S  FD         LIL                    D    K  +  +T   
Sbjct: 260 SFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEML 319

Query: 316 QNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPV 375
           +NP    H Y  +Y +S++ I +G +++  P        +G GG ++DSG+TFT +    
Sbjct: 320 ENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKF 379

Query: 376 FEAVAEAFEKQLAN-RTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGG-AKWALPL 435
           + +V E F+ ++     RA  VE ++G+ PC+ ++  ++V+ P L+  F G  +   LP 
Sbjct: 380 YNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLHFAGNRSSVTLPR 439

Query: 436 NNYFALVSSSG--------VACLTVVTHKEASGGGSGPSVILGAFQQQNFYVEYDLVNER 458
            NYF      G        + CL ++   + S    G   ILG +QQQ F V YDL+N R
Sbjct: 440 RNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRR 492

[Close]

BLAST of Carg16203 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.2e-38
Identity = 122/403 (30.27%), Postives = 180/403 (44.67%), Query Frame = 0


Query: 65  PKSNSVSKSPLSPHSYGA--YSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSF 124
           P+    S S +S  S G+  Y   L  GTP + ++++ DTGS +VW  C     C +C +
Sbjct: 122 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP---CRRC-Y 181

Query: 125 PKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAV 184
            + DP+    F P+ S +   + C +P C        +     CN + + C      Y V
Sbjct: 182 SQSDPI----FDPRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTC-----LYQV 241

Query: 185 QYGSGS-TAGLLLSETLDFPDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMG 244
            YG GS T G   +ETL F   ++    +GC   +       +G+ G G+G  S P Q G
Sbjct: 242 SYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTG 301

Query: 245 LK---KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLS 304
            +   KF+YCL  R     P S   ++    A +    +TP   NP +       +YY+ 
Sbjct: 302 HRFNQKFSYCLVDRSASSKPSS---VVFGNAAVSRIARFTPLLSNPKLDT-----FYYVG 361

Query: 305 IRKILVGNQDVK-VPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRT 364
           +  I VG   V  V          G+GG IIDSG++ T + +P + A+ +AF        
Sbjct: 362 LLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLK 421

Query: 365 RATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVV 424
           RA D         CFD+S    V+ P ++  F+ GA  +LP  NY   V ++G  C    
Sbjct: 422 RAPDFSL---FDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCF--- 481

Query: 425 THKEASGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
               A  G  G   I+G  QQQ F V YDL + R+GF    C+
Sbjct: 482 ----AFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Carg16203 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.6e-37
Identity = 127/386 (32.90%), Postives = 177/386 (45.85%), Query Frame = 0


Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   ++ GTP  +   I DTGS L+W  C     C+QC F +  P+    F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQC-FSQPTPI----FNPQDSSS 153

Query: 141 SKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGSTA-GLLLSETLD 200
              + C++  C      D+ S         E C      Y   YG GST  G + +ET  
Sbjct: 154 FSTLPCESQYC-----QDLPS---------ETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 201 FPDQKIPNFVVGC----SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSP 260
           F    +PN   GC            +G+ G G G  SLPSQ+G+ +F+YC+ S     SP
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSSP 273

Query: 261 HSGELILDSGGAKTGDLTYTPFRQ--NPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYL 320
            +  L   + G   G  + T      NP+        YYY++++ I VG  ++ +P    
Sbjct: 274 STLALGSAASGVPEGSPSTTLIHSSLNPT--------YYYITLQGITVGGDNLGIPSSTF 333

Query: 321 VPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLRPCFDISK 380
               DG+GG IIDSG+T T++ +  + AVA+AF  Q+      T  ES++GL  CF    
Sbjct: 334 QLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI---NLPTVDESSSGLSTCFQQPS 393

Query: 381 EKS-VEFPELIFQFKGGAKWALPLNNYFALVS-SSGVACLTVVTHKEASGGGSGPSV-IL 440
           + S V+ PE+  QF GG    L L     L+S + GV CL       A G  S   + I 
Sbjct: 394 DGSTVQVPEISMQFDGG---VLNLGEQNILISPAEGVICL-------AMGSSSQLGISIF 435

Query: 441 GAFQQQNFYVEYDLVNERLGFRQQSC 457
           G  QQQ   V YDL N  + F    C
Sbjct: 454 GNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Carg16203 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 4.8e-34
Identity = 129/456 (28.29%), Postives = 203/456 (44.52%), Query Frame = 0


Query: 13  LLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNS-VS 72
           LLL  ++A V A A  +++  +   HPSS  PL+++  LA     R   + +  + + VS
Sbjct: 9   LLLLLLAATVAAAAAELSVYHNV--HPSSPSPLESIIALARDDDARLLFLSSKAATAGVS 68

Query: 73  KSPL-SPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRI 132
            +P+ S  +  +Y      G+P Q L L  DT +   W  C+    C  C    +     
Sbjct: 69  SAPVASGQAPPSYVVRAGLGSPSQQLLLALDTSADATWAHCSP---CGTCPSSSL----- 128

Query: 133 PRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGSTA 192
             F P  SSS   + C +  C    G    +     +      T    A++  +   S  
Sbjct: 129 --FAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADASFQ 188

Query: 193 GLLLSETLDFPDQKIPNFVVGCSFLSIHQPS------GIAGFGRGSESLPSQMGL---KK 252
             L S+TL      IPN+  GC   S+  P+      G+ G GRG  +L SQ G      
Sbjct: 189 AALASDTLRLGKDAIPNYTFGC-VSSVTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGV 248

Query: 253 FAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILV 312
           F+YCL S  +     SG L L +GG +   + YTP  +NP  S+      YY+++  + V
Sbjct: 249 FSYCLPS--YRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSS-----LYYVNVTGLSV 308

Query: 313 GNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVES 372
           G+  VKVP       +    G+++DSG+  T    PV+ A+ E F +Q+A  +  T   S
Sbjct: 309 GHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYT---S 368

Query: 373 ATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEASG 432
                 CF+  +  +   P +     GG   ALP+ N     S++ +ACL +    EA  
Sbjct: 369 LGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLACLAMA---EAPQ 428

Query: 433 GGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
             +    ++   QQQN  V +D+ N R+GF ++SC+
Sbjct: 429 NVNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

[Close]

BLAST of Carg16203 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 8.1e-34
Identity = 124/429 (28.90%), Postives = 183/429 (42.66%), Query Frame = 0


Query: 38  HPSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSPLSPHSY---GAYSAPLSFGTPPQ 97
           H  S   L     L  A +  + +++  ++     S +    Y   G Y   LS GTP Q
Sbjct: 47  HVDSGKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQ 106

Query: 98  TLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWV 157
               I DTGS L+W  C     C+QC F +  P+    F P+ SSS   + C +  C  +
Sbjct: 107 PFSAIMDTGSDLIWTQCQP---CTQC-FNQSTPI----FNPQGSSSFSTLPCSSQLCQAL 166

Query: 158 FGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGS-TAGLLLSETLDFPDQKIPNFVVGC- 217
             P               C+     Y   YG GS T G + +ETL F    IPN   GC 
Sbjct: 167 SSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCG 226

Query: 218 ---SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGELILDSGGAKT 277
                      +G+ G GRG  SLPSQ+ + KF+YC+       S  S  L+     + T
Sbjct: 227 ENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMT--PIGSSTPSNLLLGSLANSVT 286

Query: 278 GDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKV-PYKYLVPGSDGSGGSIIDSG 337
                T   Q+  +       +YY+++  + VG+  + + P  + +  ++G+GG IIDSG
Sbjct: 287 AGSPNTTLIQSSQIPT-----FYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSG 346

Query: 338 STFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLRPCFDISKEKS-VEFPELIFQFK 397
           +T T+     +++V + F  Q+          S++G   CF    + S ++ P  +  F 
Sbjct: 347 TTLTYFVNNAYQSVRQEFISQI---NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFD 406

Query: 398 GGAKWALPLNNYFALVSSSGVACLTVVTHKEASGGGSGPSVILGAFQQQNFYVEYDLVNE 457
           GG    LP  NYF +  S+G+ CL       A G  S    I G  QQQN  V YD  N 
Sbjct: 407 GG-DLELPSENYF-ISPSNGLICL-------AMGSSSQGMSIFGNIQQQNMLVVYDTGNS 434

[Close]

BLAST of Carg16203 vs. ExPASy TrEMBL
Match: A0A6J1FJU4 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111446087 PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 2.7e-266
Identity = 455/457 (99.56%), Postives = 456/457 (99.78%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSA PHPSSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEA+
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

[Close]

BLAST of Carg16203 vs. ExPASy TrEMBL
Match: A0A6J1KTP0 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111498572 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.8e-260
Identity = 446/457 (97.59%), Postives = 448/457 (98.03%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVD NANSITLPLSAFPH SSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKS LSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKC WVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGG KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTHKEA+
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQ+CS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 457

[Close]

BLAST of Carg16203 vs. ExPASy TrEMBL
Match: A0A6J1F3G5 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111441834 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 6.1e-234
Identity = 404/459 (88.02%), Postives = 423/459 (92.16%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MA PPPLCF YILLL SVSAI D   N ITLPLS+FPH SSSDPLQ LNFLASASQNRAH
Sbjct: 1   MAPPPPLCFFYILLLSSVSAIAD--TNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAH 60

Query: 61  QIKTP--KSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCS 120
           QIK P  KSNSVSKSPLSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVW PCTSKYLCS
Sbjct: 61  QIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCS 120

Query: 121 QCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCP 180
           +CSFPKIDP RIPRF+PKLSSSSKLVGCQNPKCAW+FGPDVKSQCR+CNPKTENCTQTCP
Sbjct: 121 ECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCP 180

Query: 181 AYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240
           AY VQYGSGSTAGLLLSETLDFP++KI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG
Sbjct: 181 AYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240

Query: 241 LKKFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRK 300
           LKKFAYCLASRKFDDSPH+GELILDS GAKT  LTYTPFRQNPSVSNHAYKEYYYL+IRK
Sbjct: 241 LKKFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRK 300

Query: 301 ILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATD 360
           I VGN+ VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPVFEAVA+  EKQLANRTRATD
Sbjct: 301 IFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATD 360

Query: 361 VESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKE 420
           VES TGLRPCFDISK+KSVEFPEL F  KGGAKWA PL+NYFALVSSSGVACLTVVTHK 
Sbjct: 361 VESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKA 420

Query: 421 ASGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           A  GG GPS+ILGAFQQQNFYVEYDLVN+++GFRQQ+CS
Sbjct: 421 AESGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of Carg16203 vs. ExPASy TrEMBL
Match: A0A6J1IXY3 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.2e-233
Identity = 400/459 (87.15%), Postives = 422/459 (91.94%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MA PPPLCF YILL+ SVSAI D   N IT+PLS+FPH SSSDPLQ LNFLASASQNRAH
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIAD--TNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAH 60

Query: 61  QIKTPK--SNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCS 120
           QIK PK  SNSVSKSPLSPHSYGAYS PLSFGTPPQTLHLIFDTGSSLVW PCTSKYLCS
Sbjct: 61  QIKAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCS 120

Query: 121 QCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCP 180
           +CSFPKIDP  IPRF+PKLSS+SKLVGCQNPKCAW+FGPDVKSQCR+CNPKTENCTQTCP
Sbjct: 121 ECSFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCP 180

Query: 181 AYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240
           AY VQYGSGSTAGLLLSETLDFPD+K  NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG
Sbjct: 181 AYVVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240

Query: 241 LKKFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRK 300
           LKKFAYCLASRKFDDSPH+GELILDS GAKT  L+YTPFRQNPSVSNHAYKEYYYL+IRK
Sbjct: 241 LKKFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRK 300

Query: 301 ILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATD 360
           I VG + VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPVFEAVA+  EKQLANRTRATD
Sbjct: 301 IFVGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATD 360

Query: 361 VESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKE 420
           VES TGLRPCFDISK+KSVEFPEL FQ KGGAKW LPL+NYFALVSSSGVACLTVVTHK 
Sbjct: 361 VESLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKT 420

Query: 421 ASGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           A  GG GPS+ILGAFQQQNFYVEYDLVN+++GFRQQ+CS
Sbjct: 421 ADSGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

[Close]

BLAST of Carg16203 vs. ExPASy TrEMBL
Match: A0A5A7TRK2 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004410 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.0e-232
Identity = 397/457 (86.87%), Postives = 423/457 (92.56%), Query Frame = 0


Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60
           MA+P PL F YILL  S+SAI  +N+N ITLPL++ PH SSSDPLQ L FLASAS+NRAH
Sbjct: 1   MASPSPLSFFYILLFSSLSAI--SNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           +IKTPKSNSVSKSPLSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVWFPCTS+YLC++C
Sbjct: 61  RIKTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTEC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDP  IPRFVPKLSSSSKLVGCQNPKCAW+FGPDVKSQCR+CNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFP++KIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDS HSG+LILDS G KT  LTYT FRQNPSVSNHAYKEYYYL+IRKI+
Sbjct: 241 KFAYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKII 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQ VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPV + VA+ FEKQLANRTRATDVE
Sbjct: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420
           + TGLRPCFD+SKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH    
Sbjct: 361 TLTGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTED 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGG GPSVILGAFQQQNFYVEYDLVNERLGFR+Q+C+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of Carg16203 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 530.4 bits (1365), Expect = 1.4e-150
Identity = 266/472 (56.36%), Postives = 338/472 (71.61%), Query Frame = 0


Query: 9   FSYILLLFSVSAIVDANANSITLPLSAFPHPSSS--DPLQNLNFLASASQNRAHQIK--- 68
           F + L+  SV        +++ LPLS F H   S  DP  +L  LA +S  RAH++K   
Sbjct: 6   FFFFLIFLSV-------VSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGT 65

Query: 69  ------------TPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPC 128
                       T  S +V KSPLS  SYG YS  LSFGTP QT+  +FDTGSSLVW PC
Sbjct: 66  SIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPC 125

Query: 129 TSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTE 188
           TS+YLCS C F  +DP  IPRF+PK SSSSK++GCQ+PKC +++GP+V  QCR C+P T 
Sbjct: 126 TSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTR 185

Query: 189 NCTQTCPAYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSE 248
           NCT  CP Y +QYG GSTAG+L++E LDFPD  +P+FVVGCS +S  QP+GIAGFGRG  
Sbjct: 186 NCTVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPV 245

Query: 249 SLPSQMGLKKFAYCLASRKFDDSPHSGELILDSG-----GAKTGDLTYTPFRQNPSVSNH 308
           SLPSQM LK+F++CL SR+FDD+  + +L LD+G     G+KT  LTYTPFR+NP+VSN 
Sbjct: 246 SLPSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNK 305

Query: 309 AYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAF 368
           A+ EYYYL++R+I VG + VK+PYKYL PG++G GGSI+DSGSTFTFM++PVFE VAE F
Sbjct: 306 AFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEF 365

Query: 369 EKQLANRTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSS 428
             Q++N TR  D+E  TGL PCF+IS +  V  PELIF+FKGGAK  LPL+NYF  V ++
Sbjct: 366 ASQMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNT 425

Query: 429 GVACLTVVTHKEAS-GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
              CLTVV+ K  +  GG+GP++ILG+FQQQN+ VEYDL N+R GF ++ CS
Sbjct: 426 DTVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of Carg16203 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 196.1 bits (497), Expect = 6.4e-50
Identity = 147/489 (30.06%), Postives = 220/489 (44.99%), Query Frame = 0


Query: 16  FSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSPL 75
           FSVS++       ++  LS   H  SS PL  L   +S S  R  +    +       P+
Sbjct: 20  FSVSSLSTPLLLHLSHSLSTSKH--SSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPI 79

Query: 76  SPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVP 135
           S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C    + P        
Sbjct: 80  S--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTCILCESKPLPPSP----PS 139

Query: 136 KLSSSSKLVGCQNPKCAWVFGPDVKSQ---CRNCN---PKTENCTQT---CPAYAVQYGS 195
            LSSS+  V C +P C+        S      NC     +T +C  +   CP +   YG 
Sbjct: 140 SLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 199

Query: 196 GSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGL------K 255
           GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP+Q+ +       
Sbjct: 200 GSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGN 259

Query: 256 KFAYCLASRKFDDS--PHSGELIL--------------------DSGGAKTGDLTYTPFR 315
            F+YCL S  FD         LIL                    D    K  +  +T   
Sbjct: 260 SFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEML 319

Query: 316 QNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPV 375
           +NP    H Y  +Y +S++ I +G +++  P        +G GG ++DSG+TFT +    
Sbjct: 320 ENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKF 379

Query: 376 FEAVAEAFEKQLAN-RTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGG-AKWALPL 435
           + +V E F+ ++     RA  VE ++G+ PC+ ++  ++V+ P L+  F G  +   LP 
Sbjct: 380 YNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLHFAGNRSSVTLPR 439

Query: 436 NNYFALVSSSG--------VACLTVVTHKEASGGGSGPSVILGAFQQQNFYVEYDLVNER 458
            NYF      G        + CL ++   + S    G   ILG +QQQ F V YDL+N R
Sbjct: 440 RNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRR 492

[Close]

BLAST of Carg16203 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-49
Identity = 148/486 (30.45%), Postives = 221/486 (45.47%), Query Frame = 0


Query: 10  SYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNS 69
           +++L LF +  ++    N         P  SSS      +FL       +  + TPKS +
Sbjct: 5   THVLFLFLLITLLLNTTNKTQARQHKNPSSSSS------SFLVLTLTKSSVSLPTPKSQT 64

Query: 70  VS--KSPLSP---------HSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTS-KYLC 129
               K PLS               Y   L+ GTPPQ + +  DTGS L W PC +  + C
Sbjct: 65  QERIKKPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDC 124

Query: 130 SQCSFPKIDPLRIPR-FVPKLSSSSKLVGCQNPKCAWV------FGPDVKSQCRNCNPKT 189
            +C   K + L+ P  F P  SS+S    C +  C  +      F P   + C       
Sbjct: 125 IECYDLKNNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLK 184

Query: 190 ENCTQTCPAYAVQYGSGS-TAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRG 249
             C + CP++A  YG G   +G+L  + L    + +P F  GC   +  +P GIAGFGRG
Sbjct: 185 STCVRPCPSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRG 244

Query: 250 SESLPSQMGL--KKFAYCLASRKFDDSPH-SGELILDSGGAK---TGDLTYTPFRQNPSV 309
             SLPSQ+G   K F++C    KF ++P+ S  LIL +       T  L +TP    P  
Sbjct: 245 LLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNTP-- 304

Query: 310 SNHAYKEYYYLSIRKILVGNQ--DVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEA 369
               Y   YY+ +  I +G      +VP       S G+GG ++DSG+T+T + +P +  
Sbjct: 305 ---MYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQ 364

Query: 370 VAEAFEKQLANRTRATDVESATGLRPCFDI--------SKEKSVE--FPELIFQFKGGAK 429
           +    +  +    RAT+ ES TG   C+ +        S E  V   FP + F F   A 
Sbjct: 365 LLTTLQSTI-TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNAT 424

Query: 430 WALPL-NNYFALVSSSGVACLTVVTHKEASGGGSGPSVILGAFQQQNFYVEYDLVNERLG 457
             LP  N+++A+ + S  + +  +  +    G  GP+ + G+FQQQN  V YDL  ER+G
Sbjct: 425 LLLPQGNSFYAMSAPSDGSVVQCLLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKERIG 478

[Close]

BLAST of Carg16203 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-41
Identity = 118/383 (30.81%), Postives = 174/383 (45.43%), Query Frame = 0


Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   +  G P + ++++ DTGS + W  CT    C+ C + + +P+    F P  SSS
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP---CADC-YHQTEPI----FEPSSSSS 205

Query: 141 SKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGS-TAGLLLSETLD 200
            + + C  P+C  +      S+CRN          TC  Y V YG GS T G   +ETL 
Sbjct: 206 YEPLSCDTPQCNAL----EVSECRNA---------TC-LYEVSYGDGSYTVGDFATETLT 265

Query: 201 FPDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPH 260
                + N  VGC   +       +G+ G G G  +LPSQ+    F+YCL  R  D +  
Sbjct: 266 IGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSAS- 325

Query: 261 SGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPG 320
                +D G + + D    P      + NH    +YYL +  I VG + +++P       
Sbjct: 326 ----TVDFGTSLSPDAVVAPL-----LRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMD 385

Query: 321 SDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLR---PCFDISK 380
             GSGG IIDSG+  T +   ++ ++ ++F K         D+E A G+     C+++S 
Sbjct: 386 ESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVK------GTLDLEKAAGVAMFDTCYNLSA 445

Query: 381 EKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEASGGGSGPSVILGAF 440
           + +VE P + F F GG   ALP  NY   V S G  CL       A    +    I+G  
Sbjct: 446 KTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCL-------AFAPTASSLAIIGNV 483

Query: 441 QQQNFYVEYDLVNERLGFRQQSC 457
           QQQ   V +DL N  +GF    C
Sbjct: 506 QQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of Carg16203 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 166.8 bits (421), Expect = 4.1e-41
Identity = 126/398 (31.66%), Postives = 180/398 (45.23%), Query Frame = 0


Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   +  GTPP+   LI DTGS L W  C   Y C   +    D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 141 SKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGS-TAGLLLSETLD 200
            K + C +P+C+ +  PD   QC + N       Q+CP Y   YG  S T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 201 F---------PDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGL---KKFAY 260
                      + K+ N + GC   +       SG+ G GRG  S  SQ+       F+Y
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 261 CLASRKFDDSPHSGELIL--DSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVG 320
           CL  R   ++  S +LI   D       +L +T F        ++ + +YY+ I+ ILVG
Sbjct: 338 CLVDRN-SNTNVSSKLIFGEDKDLLNHTNLNFTSFVNG---KENSVETFYYIQIKSILVG 397

Query: 321 NQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAF-EKQLANRTRATDVES 380
            + + +P +     SDG GG+IIDSG+T ++  +P +E +   F EK   N     D   
Sbjct: 398 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 457

Query: 381 ATGLRPCFDIS--KEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEA 440
              L PCF++S  +E ++  PEL   F  G  W  P  N F  +S   + CL ++     
Sbjct: 458 ---LDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSED-LVCLAIL----- 517

Query: 441 SGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
            G       I+G +QQQNF++ YD    RLGF    C+
Sbjct: 518 -GTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKCA 525

The following BLAST results are available for this feature:

BLAST of Carg16203 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028143.1	5.1e-267	100.00	Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022940517.1	5.6e-266	99.56	probable aspartyl protease At4g16563 [Cucurbita moschata]	[more]
KAG6596604.1	7.3e-266	99.56	Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023540611.1	4.4e-263	98.47	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_023005632.1	7.8e-260	97.59	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]

BLAST of Carg16203 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	8.9e-49	30.06	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LNJ3	3.2e-38	30.27	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	1.6e-37	32.90	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q6F4N5	4.8e-34	28.29	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]
Q766C3	8.1e-34	28.90	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of Carg16203 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FJU4	2.7e-266	99.56	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114460...	[more]
A0A6J1KTP0	3.8e-260	97.59	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111498572...	[more]
A0A6J1F3G5	6.1e-234	88.02	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114418...	[more]
A0A6J1IXY3	5.2e-233	87.15	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640...	[more]
A0A5A7TRK2	2.0e-232	86.87	Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Carg16203 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	1.4e-150	56.36	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	6.4e-50	30.06	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	1.8e-49	30.45	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	1.1e-41	30.81	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	4.1e-41	31.66	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 323..334 score: 29.09 coord: 428..443 score: 30.89 coord: 89..109 score: 52.78
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 63..262 e-value: 5.3E-35 score: 123.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 267..457 e-value: 2.6E-51 score: 176.0
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 77..456
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 292..452 e-value: 3.0E-35 score: 121.4
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 83..260 e-value: 9.0E-30 score: 104.2
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 5..456
None	No IPR available	PANTHER	PTHR47967:SF36	BNACNNG47670D PROTEIN	coord: 5..456
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 98..109
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 83..452 score: 36.263775
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 82..456 e-value: 8.02473E-82 score: 251.799

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16203-RA	Carg16203-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity