Carg16175 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg16175 vs. NCBI nr
Match: XP_022943442.1 (E3 ubiquitin-protein ligase UPL3-like [Cucurbita moschata] >KAG6571021.1 E3 ubiquitin-protein ligase UPL3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3577.7 bits (9276), Expect = 0.0e+00
Identity = 1884/1890 (99.68%), Postives = 1886/1890 (99.79%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60
            METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60

Query: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120
            SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG
Sbjct: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120

Query: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180
            GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK
Sbjct: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180

Query: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240
            ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL
Sbjct: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240

Query: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300
            AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL
Sbjct: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300

Query: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360
            RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV
Sbjct: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360

Query: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420
            LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR
Sbjct: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420

Query: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480
            LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE
Sbjct: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480

Query: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540
            LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL
Sbjct: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540

Query: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600
            HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL
Sbjct: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600

Query: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660
            AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD
Sbjct: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660

Query: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720
            NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA
Sbjct: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720

Query: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780
            CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI
Sbjct: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780

Query: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840
            ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK
Sbjct: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840

Query: 841  IRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900
            IRQQVLKRFKS VSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS
Sbjct: 841  IRQQVLKRFKSFVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900

Query: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960
            ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ
Sbjct: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960

Query: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020
            KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS
Sbjct: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020

Query: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080
            KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS
Sbjct: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080

Query: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF 1140
            LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF
Sbjct: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF 1140

Query: 1141 GSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200
            GSSSRTAT+RGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG
Sbjct: 1141 GSSSRTATVRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200

Query: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260
            GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI
Sbjct: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260

Query: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLPCD 1320
            YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN   +FHQMSLLDSILQGKLPCD
Sbjct: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSNSESQFHQMSLLDSILQGKLPCD 1320

Query: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380
            LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV
Sbjct: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380

Query: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440
            NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR
Sbjct: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440

Query: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500
            LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE
Sbjct: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500

Query: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560
            VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI
Sbjct: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560

Query: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620
            DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK
Sbjct: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620

Query: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680
            LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL
Sbjct: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680

Query: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740
            CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT
Sbjct: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740

Query: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG 1800
            ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG
Sbjct: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG 1800

Query: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY 1860
            FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY
Sbjct: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY 1860

Query: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            LKLPPYSTKEIMYKKLMYAINEGQGSFDLS
Sbjct: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1890

BLAST of Carg16175 vs. NCBI nr
Match: XP_023512904.1 (E3 ubiquitin-protein ligase UPL3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3561.2 bits (9233), Expect = 0.0e+00
Identity = 1874/1890 (99.15%), Postives = 1882/1890 (99.58%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60
            METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60

Query: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120
            SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG
Sbjct: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120

Query: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180
            GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK
Sbjct: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180

Query: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240
            ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL
Sbjct: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240

Query: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300
            AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL
Sbjct: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300

Query: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360
            RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV
Sbjct: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360

Query: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420
            LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR
Sbjct: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420

Query: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480
            LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE
Sbjct: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480

Query: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540
            LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPE+SAREKLLKDQPELL
Sbjct: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEVSAREKLLKDQPELL 540

Query: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600
            HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL
Sbjct: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600

Query: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660
            AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD
Sbjct: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660

Query: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720
            NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGG+VPQGSVEVPSINSNLRTSVSA
Sbjct: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGTVPQGSVEVPSINSNLRTSVSA 720

Query: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780
            CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGS IDDI
Sbjct: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSRIDDI 780

Query: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840
            ITNK+QYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK
Sbjct: 781  ITNKEQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840

Query: 841  IRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900
            IRQQVLKRFKS VSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS
Sbjct: 841  IRQQVLKRFKSFVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900

Query: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960
            ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ
Sbjct: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960

Query: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020
            KLSVSGANSDSGTTPPGTVAPSGLKSTP+STTCRYSTRSRSSMTIGERAGKELSQDKSTS
Sbjct: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPNSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020

Query: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080
            KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS
Sbjct: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080

Query: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF 1140
            LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQ+HSTF
Sbjct: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQVHSTF 1140

Query: 1141 GSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200
            GSSSRTAT+RGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG
Sbjct: 1141 GSSSRTATVRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200

Query: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260
            GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI
Sbjct: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260

Query: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLPCD 1320
            YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN   +FHQMSLLDSILQGKLPCD
Sbjct: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSNSESQFHQMSLLDSILQGKLPCD 1320

Query: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380
            LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKIT LDELGGIGGKVPHEEFV
Sbjct: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITMLDELGGIGGKVPHEEFV 1380

Query: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440
            NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR
Sbjct: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440

Query: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500
            LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE
Sbjct: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500

Query: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560
            VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI
Sbjct: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560

Query: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620
            DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK
Sbjct: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620

Query: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680
            LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNT+SNLSFRGIPVEDL
Sbjct: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTLSNLSFRGIPVEDL 1680

Query: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740
            CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT
Sbjct: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740

Query: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG 1800
            ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQRG
Sbjct: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQRG 1800

Query: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY 1860
            FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATG SESADDDLPSVMTCANY
Sbjct: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGASESADDDLPSVMTCANY 1860

Query: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            LKLPPYSTKEIMYKKLMYAINEGQGSFDLS
Sbjct: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1890

BLAST of Carg16175 vs. NCBI nr
Match: XP_022987043.1 (E3 ubiquitin-protein ligase UPL3-like [Cucurbita maxima])

HSP 1 Score: 3539.6 bits (9177), Expect = 0.0e+00
Identity = 1867/1890 (98.78%), Postives = 1874/1890 (99.15%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60
            METRSRKRAEASSAAPSSSSSGPTTRS+KRARLSGTFSSNLAASTLSISTRSRSTRNQEP
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPTTRSTKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60

Query: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120
            SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG
Sbjct: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120

Query: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180
            GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK
Sbjct: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180

Query: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240
            ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL
Sbjct: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240

Query: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300
            AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL
Sbjct: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300

Query: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360
            RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV
Sbjct: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360

Query: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420
            LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR
Sbjct: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420

Query: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480
            LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE
Sbjct: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480

Query: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540
            LLPPLPQGTISFP NYNLFVKGPVIKKPSTSGSVKEEDPTDS PE+SAREKLLKDQPELL
Sbjct: 481  LLPPLPQGTISFPSNYNLFVKGPVIKKPSTSGSVKEEDPTDSVPEVSAREKLLKDQPELL 540

Query: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600
            HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL
Sbjct: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600

Query: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660
            AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNT+TQVASVEKD
Sbjct: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTSTQVASVEKD 660

Query: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720
            NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA
Sbjct: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720

Query: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780
            CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGS IDDI
Sbjct: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSRIDDI 780

Query: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840
            ITNK+QYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK
Sbjct: 781  ITNKEQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840

Query: 841  IRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900
            IRQQVLKRFKS VSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS
Sbjct: 841  IRQQVLKRFKSFVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900

Query: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960
            ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ
Sbjct: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960

Query: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020
            KLSVSGANSDSGT PPGTVAPSGLKSTPS TTCRYSTRSRSSMTIGERAGKELSQDKSTS
Sbjct: 961  KLSVSGANSDSGTMPPGTVAPSGLKSTPSPTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020

Query: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080
            KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS
Sbjct: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080

Query: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF 1140
            LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQI STF
Sbjct: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIPSTF 1140

Query: 1141 GSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200
            GSSSRTAT RGSNSPDHRGGNSFSARG MTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG
Sbjct: 1141 GSSSRTATARGSNSPDHRGGNSFSARGGMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200

Query: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260
            GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI
Sbjct: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260

Query: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLPCD 1320
            YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN   +FHQMSLLDSILQGKLPCD
Sbjct: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSNSESQFHQMSLLDSILQGKLPCD 1320

Query: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380
            LDKSN TYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV
Sbjct: 1321 LDKSNSTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380

Query: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440
            NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR
Sbjct: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440

Query: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500
            LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE
Sbjct: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500

Query: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560
            VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI
Sbjct: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560

Query: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620
            DIIQSPLGLFP+PW PNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK
Sbjct: 1561 DIIQSPLGLFPQPWQPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620

Query: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680
            LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL
Sbjct: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680

Query: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740
            CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT
Sbjct: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740

Query: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG 1800
            ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQRG
Sbjct: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQRG 1800

Query: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY 1860
            FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTA+NAANSATG SESADDDLPSVMTCANY
Sbjct: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTASNAANSATGASESADDDLPSVMTCANY 1860

Query: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            LKLPPYSTKEIMYKKLMYAINEGQGSFDLS
Sbjct: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1890

BLAST of Carg16175 vs. NCBI nr
Match: XP_004147040.1 (E3 ubiquitin-protein ligase UPL3 [Cucumis sativus] >KAE8651898.1 hypothetical protein Csa_006194 [Cucumis sativus])

HSP 1 Score: 3371.3 bits (8740), Expect = 0.0e+00
Identity = 1779/1892 (94.03%), Postives = 1824/1892 (96.41%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNL-AASTLSISTRSRSTRNQE 60
            METRSRKRAEASSAAPSSSSSGP TRS+KR+RLS T SSNL AASTLSISTRSRSTR QE
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPNTRSTKRSRLSATSSSNLAAASTLSISTRSRSTRTQE 60

Query: 61   PSATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSG 120
            PSAT+TPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNA+QSFGLNIEGSG
Sbjct: 61   PSATTTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNADQSFGLNIEGSG 120

Query: 121  GGDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK 180
            GG+DDDNDSEGG+GVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK
Sbjct: 121  GGEDDDNDSEGGIGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK 180

Query: 181  KILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML 240
            KILSGLRADGEEG+QVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML
Sbjct: 181  KILSGLRADGEEGKQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML 240

Query: 241  LAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTAC 300
            LAARALTHLCD LPSSCAAVVHYGAV CFCARLLTIEYMDLAEQSLQALKKISQEHPTAC
Sbjct: 241  LAARALTHLCDVLPSSCAAVVHYGAVPCFCARLLTIEYMDLAEQSLQALKKISQEHPTAC 300

Query: 301  LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK 360
            LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK
Sbjct: 301  LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK 360

Query: 361  VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLI 420
            VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTL SATYTGLI
Sbjct: 361  VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLGSATYTGLI 420

Query: 421  RLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLAN 480
            RLLSTCASGSALG KTLLLLGISGILKDILSGSGVSTN+SVSPALNRPQEQIFEIVNLAN
Sbjct: 421  RLLSTCASGSALGAKTLLLLGISGILKDILSGSGVSTNASVSPALNRPQEQIFEIVNLAN 480

Query: 481  ELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPEL 540
            ELLPPLPQGTISFPPN+N+ VKGPVIKKPSTSGSVKEEDPTDSAPE+SAREKLLKDQPEL
Sbjct: 481  ELLPPLPQGTISFPPNFNMLVKGPVIKKPSTSGSVKEEDPTDSAPEVSAREKLLKDQPEL 540

Query: 541  LHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV 600
            L QFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV
Sbjct: 541  LLQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV 600

Query: 601  LAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEK 660
            LAWKDPHILIP+LQIAEILMEKL ETFSKMFLREGVVYAVDQLILANNQNT++Q ASVEK
Sbjct: 601  LAWKDPHILIPALQIAEILMEKLHETFSKMFLREGVVYAVDQLILANNQNTSSQSASVEK 660

Query: 661  DNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVS 720
            D+ SASG SSRTRRYRRRSGNM S+GSSLDENKN VSG  VPQGSVEVPSINSNLR+SVS
Sbjct: 661  DSTSASGTSSRTRRYRRRSGNMNSDGSSLDENKNSVSGSGVPQGSVEVPSINSNLRSSVS 720

Query: 721  ACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDD 780
            +CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLC KLNAGID QKSKSKGK KASGS +DD
Sbjct: 721  SCANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCFKLNAGIDDQKSKSKGKLKASGSRLDD 780

Query: 781  IITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELP 840
            IITNK++YLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLL+YFSCGYFSKGRI + ELP
Sbjct: 781  IITNKEEYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLNYFSCGYFSKGRISDVELP 840

Query: 841  KIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG 900
            K+RQQVLKRFKS +SVAL  SINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG
Sbjct: 841  KLRQQVLKRFKSFISVALPGSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG 900

Query: 901  SARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSE- 960
            SARLSSGLSVLSQPFKLRLCRA GEK LRDYSSNIVLIDPLASLAAVEEFLWPRVQ+SE 
Sbjct: 901  SARLSSGLSVLSQPFKLRLCRAQGEKSLRDYSSNIVLIDPLASLAAVEEFLWPRVQKSES 960

Query: 961  GQKLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKS 1020
            GQK S SGANSDSGTTP G VAPSGL STPSST  RYSTRSRSSMTIGERAGKE SQ+K+
Sbjct: 961  GQKPSASGANSDSGTTPSGNVAPSGLNSTPSSTARRYSTRSRSSMTIGERAGKESSQEKN 1020

Query: 1021 TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQID 1080
            TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDL+SIQID
Sbjct: 1021 TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLTSIQID 1080

Query: 1081 DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHS 1140
            DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDT EDGDAGPATSD QIHS
Sbjct: 1081 DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTVEDGDAGPATSDGQIHS 1140

Query: 1141 TFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPL 1200
            TFGSSSR AT+RGS+SPDHR GNSFS+RG M+FAAAAMAGLGPANGRGFRGGRDPQG PL
Sbjct: 1141 TFGSSSRAATVRGSSSPDHRSGNSFSSRGGMSFAAAAMAGLGPANGRGFRGGRDPQGRPL 1200

Query: 1201 FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWG 1260
            FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAG G+DFLSNDGSSLWG
Sbjct: 1201 FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGTGSDFLSNDGSSLWG 1260

Query: 1261 DIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLP 1320
            DIYTITYQRADNQSERA LAGESSSSKSKSTKCVSTSNSN   +FHQMSLLDSILQGKLP
Sbjct: 1261 DIYTITYQRADNQSERAVLAGESSSSKSKSTKCVSTSNSNSESQFHQMSLLDSILQGKLP 1320

Query: 1321 CDLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEE 1380
            CD DKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKIT LDELGG+GGKVPHEE
Sbjct: 1321 CDFDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITALDELGGVGGKVPHEE 1380

Query: 1381 FVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL 1440
            F+N+KLTPKL RQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL
Sbjct: 1381 FINNKLTPKLARQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL 1440

Query: 1441 YRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYF 1500
            YRLHQQ GADGLGTVNEREGRVGRLQRQKVRVSRNRILDSA KVMEMYS+QKAVLEVEYF
Sbjct: 1441 YRLHQQQGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYF 1500

Query: 1501 GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAG 1560
            GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQES DSGEDGQ RKPK GS+L SDA 
Sbjct: 1501 GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESTDSGEDGQARKPKGGSRLTSDAA 1560

Query: 1561 NIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF 1620
            NIDIIQSPLGLFPRPWP NADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF
Sbjct: 1561 NIDIIQSPLGLFPRPWPANADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF 1620

Query: 1621 YKLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVE 1680
            YKLVLGQDLDL+DILSFD ELGKTLQELQALVCRKQYLG ++GDNQNTISNL+FRGIPVE
Sbjct: 1621 YKLVLGQDLDLHDILSFDAELGKTLQELQALVCRKQYLGSLNGDNQNTISNLTFRGIPVE 1680

Query: 1681 DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFD 1740
            DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFT GFNQVFD
Sbjct: 1681 DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTAGFNQVFD 1740

Query: 1741 ITALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQ 1800
            ITAL IFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQ
Sbjct: 1741 ITALHIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQ 1800

Query: 1801 RGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCA 1860
            R FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATG SESADDDLPSVMTCA
Sbjct: 1801 RAFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGASESADDDLPSVMTCA 1860

Query: 1861 NYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            NYLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1861 NYLKLPPYSTKEIMYKKLIYAINEGQGSFDLS 1892

BLAST of Carg16175 vs. NCBI nr
Match: XP_008457685.1 (PREDICTED: E3 ubiquitin-protein ligase UPL3 [Cucumis melo])

HSP 1 Score: 3369.3 bits (8735), Expect = 0.0e+00
Identity = 1779/1892 (94.03%), Postives = 1821/1892 (96.25%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNL-AASTLSISTRSRSTRNQE 60
            METRSRKRAEASSAAPSSSSSGPTTRS+KRARLS T SSNL AASTLSISTRSRSTR QE
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPTTRSTKRARLSATSSSNLAAASTLSISTRSRSTRTQE 60

Query: 61   PSATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSG 120
            PSAT+TPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNA+QSFGLNIEGSG
Sbjct: 61   PSATTTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNADQSFGLNIEGSG 120

Query: 121  GGDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK 180
            GG+DDDNDSEGGMGVLQ NLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK
Sbjct: 121  GGEDDDNDSEGGMGVLQHNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK 180

Query: 181  KILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML 240
            KILSGLRADGEEG+QVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML
Sbjct: 181  KILSGLRADGEEGKQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML 240

Query: 241  LAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTAC 300
            LAARALTHLCD LPSSCAAVVHYGAV CFCARLLTIEYMDLAEQSLQALKKISQEHPTAC
Sbjct: 241  LAARALTHLCDVLPSSCAAVVHYGAVPCFCARLLTIEYMDLAEQSLQALKKISQEHPTAC 300

Query: 301  LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK 360
            LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK
Sbjct: 301  LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK 360

Query: 361  VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLI 420
            VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTL SATYTGLI
Sbjct: 361  VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLGSATYTGLI 420

Query: 421  RLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLAN 480
            RLLSTCASGSALG KTLLLLGISGILKDILSGSGVSTN+SVSPALNRPQEQIFEIVNLAN
Sbjct: 421  RLLSTCASGSALGAKTLLLLGISGILKDILSGSGVSTNASVSPALNRPQEQIFEIVNLAN 480

Query: 481  ELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPEL 540
            ELLPPLPQGTISFPPN+N+FVKGPVIKKPSTSGSVKEEDPTDSAPE+SAREKLLKDQPEL
Sbjct: 481  ELLPPLPQGTISFPPNFNMFVKGPVIKKPSTSGSVKEEDPTDSAPEVSAREKLLKDQPEL 540

Query: 541  LHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV 600
            L QFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV
Sbjct: 541  LLQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV 600

Query: 601  LAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEK 660
            LAWKDPHILIP+LQIAEILMEKL ETFSKMFLREGVVYAVDQLILANNQNT++Q ASVEK
Sbjct: 601  LAWKDPHILIPALQIAEILMEKLHETFSKMFLREGVVYAVDQLILANNQNTSSQAASVEK 660

Query: 661  DNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVS 720
            D+ SASG SSRTRRYRRRSG M S+GSSLDENKN VSG  VPQGSVEVPSINSNLR+SVS
Sbjct: 661  DSTSASGTSSRTRRYRRRSGTMNSDGSSLDENKNSVSGSGVPQGSVEVPSINSNLRSSVS 720

Query: 721  ACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDD 780
            +CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLC KLNAGID QKSKSKGK KASGS +DD
Sbjct: 721  SCANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCFKLNAGIDDQKSKSKGKLKASGSRLDD 780

Query: 781  IITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELP 840
            IITNK++YLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLL+YFSCGYFSKGRIL+ ELP
Sbjct: 781  IITNKEEYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLNYFSCGYFSKGRILDVELP 840

Query: 841  KIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG 900
            K+RQQVLKRFKS +SVAL   INEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG
Sbjct: 841  KLRQQVLKRFKSFISVALPARINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG 900

Query: 901  SARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSE- 960
            SARLSSGLSVLSQPFKLRLCRA GEK LRDYSSNIVLIDPLASLAAVEEFLWPRVQ+SE 
Sbjct: 901  SARLSSGLSVLSQPFKLRLCRAQGEKSLRDYSSNIVLIDPLASLAAVEEFLWPRVQKSES 960

Query: 961  GQKLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKS 1020
            GQK S SGANSDSGTTP G VAPSGL STPSST  RYSTRSRSSMTIGERAGKE SQ+KS
Sbjct: 961  GQKPSASGANSDSGTTPSGNVAPSGLNSTPSSTARRYSTRSRSSMTIGERAGKESSQEKS 1020

Query: 1021 TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQID 1080
            TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQID
Sbjct: 1021 TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQID 1080

Query: 1081 DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHS 1140
            DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKL DT EDGDAGPATSD QIHS
Sbjct: 1081 DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLSDTVEDGDAGPATSDGQIHS 1140

Query: 1141 TFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPL 1200
            TFGSSSR AT+RGS+SPDHR GNSFS+RG M+FAAAAMAGLGPANGRGFRGGRDPQG PL
Sbjct: 1141 TFGSSSRAATVRGSSSPDHRSGNSFSSRGGMSFAAAAMAGLGPANGRGFRGGRDPQGRPL 1200

Query: 1201 FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWG 1260
            FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAG+DFLSNDGSSLWG
Sbjct: 1201 FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGSDFLSNDGSSLWG 1260

Query: 1261 DIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLP 1320
            DIYTITYQRADNQSERAALAGESSSSKSKSTKCVS SNSN   +FHQMSLLDSILQGKLP
Sbjct: 1261 DIYTITYQRADNQSERAALAGESSSSKSKSTKCVSASNSNSESQFHQMSLLDSILQGKLP 1320

Query: 1321 CDLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEE 1380
            CD DKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKIT LDELGG+GGKVPHEE
Sbjct: 1321 CDFDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITALDELGGVGGKVPHEE 1380

Query: 1381 FVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL 1440
            F+N+KLTPKL RQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL
Sbjct: 1381 FINNKLTPKLARQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL 1440

Query: 1441 YRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYF 1500
            YRLHQQ GADG+GTVNEREGRVGRLQRQKVRVSRNRILDSA KVMEMYS+QKAVLEVEYF
Sbjct: 1441 YRLHQQQGADGIGTVNEREGRVGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYF 1500

Query: 1501 GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAG 1560
            GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQES DSGEDGQ RK K GS+L SDA 
Sbjct: 1501 GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESTDSGEDGQARKLKGGSRLTSDAA 1560

Query: 1561 NIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF 1620
            NIDIIQSPLGLFPRPWPPNADSSDGSQ SKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF
Sbjct: 1561 NIDIIQSPLGLFPRPWPPNADSSDGSQLSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF 1620

Query: 1621 YKLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVE 1680
            YKLVLGQDLDL+DILSFD ELGKTLQELQALVCRKQYL  ++GDNQNTISNL+FRGIPVE
Sbjct: 1621 YKLVLGQDLDLHDILSFDAELGKTLQELQALVCRKQYLESINGDNQNTISNLTFRGIPVE 1680

Query: 1681 DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFD 1740
            DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFT GFNQVFD
Sbjct: 1681 DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTAGFNQVFD 1740

Query: 1741 ITALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQ 1800
            ITAL IFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQ
Sbjct: 1741 ITALHIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQ 1800

Query: 1801 RGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCA 1860
            R FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATN ANSATG SESADDDLPSVMTCA
Sbjct: 1801 RAFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNTANSATGASESADDDLPSVMTCA 1860

Query: 1861 NYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            NYLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1861 NYLKLPPYSTKEIMYKKLIYAINEGQGSFDLS 1892

BLAST of Carg16175 vs. ExPASy Swiss-Prot
Match: Q6WWW4 (E3 ubiquitin-protein ligase UPL3 OS=Arabidopsis thaliana OX=3702 GN=UPL3 PE=1 SV=1)

HSP 1 Score: 2421.4 bits (6274), Expect = 0.0e+00
Identity = 1343/1917 (70.06%), Postives = 1564/1917 (81.59%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSS---------SGPTTRSSKRARLSGTFSSNLAASTLSISTR 60
            METRSRKRAEA+SAAPSSSS         SGPTTR SKRARLS + SS+LA +  S ST 
Sbjct: 1    METRSRKRAEATSAAPSSSSSSPPPPPSASGPTTR-SKRARLSSSSSSSLAPTPPSSSTT 60

Query: 61   SRSTRNQEPSATSTPMDSTNESSGSRRRG---KNSDKENSDKGKEKEHEVRIGDRERNAE 120
            +RS R+   +A + PMD++ +SSG RR G   + ++ +NSDKGKEKEH+VRI +RER  +
Sbjct: 61   TRS-RSSRSAAAAAPMDTSTDSSGFRRGGRGNRGNNNDNSDKGKEKEHDVRIRERERERD 120

Query: 121  QS-FGLNIEGSGGG-----DDDDNDSE-GGMGVLQQNLSTASSALQGLLRKLGAGLDDLL 180
            ++   LN++ +        +DDDNDSE G  G +  N+S+ASSALQGLLRKLGAGLDDLL
Sbjct: 121  RAREQLNMDAAAAAARSADEDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLL 180

Query: 181  PSSAIASASSSQQRGRLKKILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSF 240
            PSS I SASSS   GR+KKILSGLRA+GEEG+QVEALTQLCEMLSIGTE+SLSTFSVDSF
Sbjct: 181  PSSGIGSASSSHLNGRMKKILSGLRAEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSF 240

Query: 241  VPVLVGLLNHESNPDIMLLAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLA 300
            VPVLVGLLNHESNPDIMLLAARALTHLCD LPSSCAAVVHYGAVSC  ARLLTIEYMDLA
Sbjct: 241  VPVLVGLLNHESNPDIMLLAARALTHLCDVLPSSCAAVVHYGAVSCLVARLLTIEYMDLA 300

Query: 301  EQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADF 360
            EQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDA+D+
Sbjct: 301  EQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDY 360

Query: 361  VMEAVPLLTNLLQYHDAKVLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTS 420
            VMEAVPLLTNLLQYHD+KVLE+AS+CLTRIAEAFA  P+KLDELCNHGLVTQAASLISTS
Sbjct: 361  VMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTS 420

Query: 421  STGGGQSTLSSATYTGLIRLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVS 480
            ++GGGQ++LS +TYTGLIRLLSTCASGS LG +TLLLLGIS ILKDIL GSGVS N+SVS
Sbjct: 421  NSGGGQASLSVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDILLGSGVSANASVS 480

Query: 481  PALNRPQEQIFEIVNLANELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTD 540
            PAL+RP +QI+EIVNLANELLPPLP+G IS P + N  VKG   KK S S S K+ED   
Sbjct: 481  PALSRPADQIYEIVNLANELLPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQED--- 540

Query: 541  SAPEMSAREKLLKDQPELLHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPE 600
               ++S REKLL DQPELL QFG+DLLP+L+QIYGSSVNG +RHKCLS IGKLMYFS+ E
Sbjct: 541  -ILKISPREKLLGDQPELLQQFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSE 600

Query: 601  MIQSLLNVTNIASFLAGVLAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQ 660
            MIQSL+  TNI+SFLAGVLAWKDP +L+P+LQ+AEILMEKLPETFSK+F+REGVV+AVDQ
Sbjct: 601  MIQSLIGDTNISSFLAGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQ 660

Query: 661  LILANNQNTATQVASVEKDNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVS-GGSV 720
            L+L    + A   +  +KDN+   G S+R+RRYRRRS N  S+G+  +E KNP S     
Sbjct: 661  LVLVGKPSHA---SPTDKDNDCVPG-SARSRRYRRRSSNANSDGNQSEEPKNPASLTIGA 720

Query: 721  PQGSVEVPSINSNLRTSVSACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGID 780
               S++ P+ +  LR +VS+CA AFK KYFP D GDV+VGVTDDLL LKNLC KL AGID
Sbjct: 721  NHNSLDTPTASFMLRETVSSCAKAFKDKYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGID 780

Query: 781  VQKSKSKGKSKASGSCIDDIITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLL 840
              K K KGKSKASG  + D   +K++YL GVISE+L E+ K DGVSTFEFIGSGVV  LL
Sbjct: 781  DHKVKGKGKSKASGPFLGDFSASKEEYLIGVISEILGEISKGDGVSTFEFIGSGVVAALL 840

Query: 841  DYFSCGYFSKGRILEAELPKIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSAL 900
            +YFSCGYFSK +I E  LPK+RQ+ L+RFK+ + VAL    NEG V PMTVL+QKLQ+AL
Sbjct: 841  NYFSCGYFSKEKISELNLPKLRQEGLRRFKAFLEVALPFDGNEGKVPPMTVLIQKLQNAL 900

Query: 901  SSLERFPVVLSHSSRSSSGSARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPL 960
            SSLERFPVVLSH SRS SGSARLSSGLS L+ P KLRLCRA GEK LRDYSSNIVLIDPL
Sbjct: 901  SSLERFPVVLSHPSRSLSGSARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPL 960

Query: 961  ASLAAVEEFLWPRVQRSEGQ-KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRS 1020
            ASLAAVEEFLWPRVQRSE   K +    N++ GT P G    S   STP+STT R+S+RS
Sbjct: 961  ASLAAVEEFLWPRVQRSESALKPAAPIGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRS 1020

Query: 1021 RSSMTIGERAGKELSQDK--STSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMK 1080
            RS++ IG+ + K+   +K  S+SKGKGK ++KPA  +K G QTRS+ ++RA +DKD QMK
Sbjct: 1021 RSAINIGDTSKKDPVHEKGTSSSKGKGKGVMKPAQADK-GPQTRSNAQKRAVLDKDTQMK 1080

Query: 1081 PVNGETTSEDEELDLSSIQIDDSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVK 1140
            P +G+++SEDEEL++S + IDD+LVIE+DDISDDEDDD++DVL DDSLP+C P+KVHDVK
Sbjct: 1081 PASGDSSSEDEELEISPVDIDDALVIEEDDISDDEDDDNEDVL-DDSLPMCTPDKVHDVK 1140

Query: 1141 LGDTAEDGDAGPATSDSQIHSTFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMA 1200
            L D+ +D   G ATS  Q++   G +S  A  R S+S D   GNS+ +RG+++FAAAAMA
Sbjct: 1141 LADSVDDD--GLATSGRQMNPASGGTSGAAAARASDSIDTGIGNSYGSRGALSFAAAAMA 1200

Query: 1201 GLGPANGRGFRGGRDPQGHPLFGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDD 1260
            GLG A+GRG RG RD  G  L   S++  KL+F++  KQL RHLTIYQAVQRQL+L+EDD
Sbjct: 1201 GLGAASGRGIRGSRDLHGRTLNRSSDEPSKLIFTAAGKQLSRHLTIYQAVQRQLMLDEDD 1260

Query: 1261 DERFAGAGNDFLSNDGSSLWGDIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNS 1320
            D+RF   G+D +S+DGS  + DIYTI YQR D+Q  R ++ G SS++ SKSTK  +T++S
Sbjct: 1261 DDRF--GGSDLVSSDGSR-FNDIYTIMYQRPDSQVNRLSVGGASSTTPSKSTKSATTNSS 1320

Query: 1321 -NFRFHQMSLLDSILQGKLPCDLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAE 1380
               + H+ SLLDSILQG+LPCDL+KSN TY++L+LLRVLEGLNQL PRLRAQ +SD+FAE
Sbjct: 1321 VESQSHRASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLCPRLRAQTLSDRFAE 1380

Query: 1381 GKITTLDELGGIGGKVPHEEFVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLF 1440
            GKIT+LD+L     KVP +EFVNSKLTPKL RQIQDALALCSGSLPSWCYQLT+ACPFLF
Sbjct: 1381 GKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQIQDALALCSGSLPSWCYQLTRACPFLF 1440

Query: 1441 PFETRRQYFYSTAFGLSRALYRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDS 1500
            PF+TRRQYFYSTAFGLSRAL RL QQ GADG G+ NERE R+GRLQRQKVRVSRNRILDS
Sbjct: 1441 PFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDS 1500

Query: 1501 AVKVMEMYSNQKAVLEVEYFGEVGTGLGPTLEFYTLLSHELQRAGLGMWRSN-----SLQ 1560
            A KVMEMYS+QKAVLEVEYFGEVGTGLGPTLEFYTLLSH+LQ+A LGMWRS+     S+Q
Sbjct: 1501 AAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKASLGMWRSSSGDKVSMQ 1560

Query: 1561 ESIDSGEDGQLRKPKDGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYF 1620
               D  EDG   KP         A N DI+ +PLGLFPRPWP  AD S+G QF KVIEYF
Sbjct: 1561 IGRDEIEDG---KP--------SAANRDIVLAPLGLFPRPWPSTADISEGGQFHKVIEYF 1620

Query: 1621 RLVGRVMAKALQDGRLLDLPLSTAFYKLVLGQDLDLYDILSFDPELGKTLQELQALVCRK 1680
            RL+GRVMAKALQDGRLLD+PLSTAFYKL+LGQ+LDL+DI+ FD ELGKTLQEL+ +V RK
Sbjct: 1621 RLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTLQELRVVVARK 1680

Query: 1681 QYLGLVDGDNQNTISNLSFRGIPVEDLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLV 1740
             YL  V GDN +TIS+L  RG  +EDL L+FT+PGYP+Y+LR GDE V+I NLEEYISLV
Sbjct: 1681 HYLEGVGGDNSSTISDLCLRGCRIEDLSLEFTLPGYPEYILRSGDEIVDITNLEEYISLV 1740

Query: 1741 LDATVKTGIMRQMEAFTTGFNQVFDITALRIFIPHELDHLLCGRRELWKADTLVDHIKFD 1800
            +DATVK G+ RQ+EAF +GFNQVFDIT+L+IF P ELD+LLCGRRELW+ +TL +HIKFD
Sbjct: 1741 VDATVKRGVTRQIEAFRSGFNQVFDITSLQIFTPSELDYLLCGRRELWEVETLAEHIKFD 1800

Query: 1801 HGYTAKSPAIVNFLEIMGELTPEQQRGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTA 1860
            HGY AKSPAI+N LEIMGELT +QQR FCQFVTGAPRLPPGGLAVLNP+LTIVRKHSST+
Sbjct: 1801 HGYNAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTS 1860

Query: 1861 TNAANSATGVSESADDDLPSVMTCANYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            + AAN A G SE+ADDDLPSVMTCANYLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1861 SAAANGA-GASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888

BLAST of Carg16175 vs. ExPASy Swiss-Prot
Match: Q9LYZ7 (E3 ubiquitin-protein ligase UPL4 OS=Arabidopsis thaliana OX=3702 GN=UPL4 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.6e-253
Identity = 627/1726 (36.33%), Postives = 877/1726 (50.81%), Query Frame = 0

Query: 171  SSQQRGRLKKILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLN 230
            SS   G+LK +L  L  + +   Q+  LT+LCE+LS  TEESLS+   +   PVLV L  
Sbjct: 104  SSGDHGKLKSLLLNLTGETDPSGQLSRLTELCEVLSFSTEESLSSVMANMLSPVLVKLAK 163

Query: 231  HESNPDIMLLAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKK 290
            HE+N DIMLLA RA+T+LCD  P S   +V +  +   C RLLTIEY+D+AEQ LQAL+K
Sbjct: 164  HENNADIMLLAIRAITYLCDVYPPSVEFLVRHDTIPALCQRLLTIEYLDVAEQCLQALEK 223

Query: 291  ISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLT 350
            IS++ P ACL AGA+MAVLS++DFFST +QRVA+ST  N+CK+L S++    M+AVP+L 
Sbjct: 224  ISRDEPVACLNAGAIMAVLSFIDFFSTSIQRVAISTVVNICKQLSSESPSPFMDAVPILC 283

Query: 351  NLLQYHDAKVLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTL 410
             LLQY D +++E+ ++CLT+IA+  + SP  LD+LC HGL+ ++  L++ +S    ++TL
Sbjct: 284  TLLQYEDRQLVENVAICLTKIADQASESPAMLDQLCRHGLINESTHLLNLNS----RTTL 343

Query: 411  SSATYTGLIRLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQ 470
            S   Y G+I +L   +SGSAL  +TL  L I   LK+I+S   +S + S +  +N    Q
Sbjct: 344  SQPVYNGVIGMLRKLSSGSALAFRTLYELNIGYSLKEIMSTYDISHSVSSTHPINACSNQ 403

Query: 471  IFEIVNLANELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSARE 530
            + E++ L  ELLP                                   P +     S +E
Sbjct: 404  VHEVLKLVIELLP---------------------------------ASPVEDNQLASEKE 463

Query: 531  KLLKDQPELLHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVT 590
              L +QP+LL QFG D+LP++IQ+  S  N  V + CLS I KL   S    I  LL  T
Sbjct: 464  SFLVNQPDLLQQFGRDMLPVMIQVLNSGANVYVSYGCLSAIHKLTCLSKSGDIVELLKNT 523

Query: 591  NIASFLAGVLAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNT 650
            N++S LAG+L+ KD H+++ +LQ+AE+L+EK  +TF   F++EGV +A++ L+ ++    
Sbjct: 524  NMSSVLAGILSRKDHHVIVVALQVAEVLLEKYRDTFLNSFIKEGVFFAIEALLSSDRGQQ 583

Query: 651  ATQVASVEKDNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSI 710
                A + +   +        + + R   +++S   +    K+                 
Sbjct: 584  NQGSADLSQKPVTKEIVKCLCQSFER---SLSSSSQTCKIEKD----------------- 643

Query: 711  NSNLRTSVSACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKS 770
                  SV   A   K  +F  +  + E G+TD L  LKNL   L+  + V         
Sbjct: 644  ------SVYVLATRIKEGFFGPEVFNSEKGLTDVLQNLKNLSVALSELMTVP-------- 703

Query: 771  KASGSCIDDIITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYF-- 830
                  ID  + + +++ + + ++++  L   + VSTFEFI SGVV  L  Y S G +  
Sbjct: 704  ------IDAHVLHDEKFFS-IWNQIMERLNGRESVSTFEFIESGVVKSLASYLSNGLYQR 763

Query: 831  --SKGRILEAELPKIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERF 890
              SKG      LP I     KRF+  V   L  S  E T    ++L+QKLQ++LSSLE F
Sbjct: 764  KLSKGGPECDSLPFIG----KRFE--VFTRLLWSDGEAT---SSLLIQKLQNSLSSLENF 823

Query: 891  PVVLSHSSRSSSGSARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAV 950
            P+VLS   +  +  A + +G        K+R  +A GE  LRDYS + V +DPL  L AV
Sbjct: 824  PIVLSQFLKQKNSFAAIPNGRCTSYPCLKVRFLKAEGETSLRDYSQDFVTVDPLCYLDAV 883

Query: 951  EEFLWPRVQRSEGQKLSVSGANSDSGTTPPGTVAP-SGLKSTPSSTTCRYSTRSRSSMTI 1010
            +++LWP+V                        + P   +++   +  C+ S    +S++ 
Sbjct: 884  DQYLWPKV-----------------------NIEPIDSVEAKDQAIECQSSQLQSTSISC 943

Query: 1011 GERAGKELSQDKSTSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTS 1070
               +   +  D  +S             + +G Q    T+         Q    + ET+S
Sbjct: 944  QAESSSPMEIDSESSDA----------SQLQGSQVEDQTQ------LPGQQNASSSETSS 1003

Query: 1071 EDEELDLSSIQIDDSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDG 1130
            E E+                                                        
Sbjct: 1004 EKEDA------------------------------------------------------- 1063

Query: 1131 DAGPATSDSQIHSTFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGR 1190
                                                                        
Sbjct: 1064 ------------------------------------------------------------ 1123

Query: 1191 GFRGGRDPQGHPLFGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAG 1250
                                P+LLF     +LDR LT+YQA+    + +E +      A 
Sbjct: 1124 -------------------VPRLLFRLEGLELDRSLTVYQAILLHKLKSESE------AT 1183

Query: 1251 NDFLSNDGSSLWGDIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSNFRFHQMS 1310
            ND      S L G  + ITY       ER+A  G+S  +        S  +  +R     
Sbjct: 1184 ND------SKLSGP-HNITY-------ERSAQLGDSRENLFPPG---SMEDDEYR----P 1243

Query: 1311 LLDSILQGKLPCDL-DKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDE 1370
             L  +   +L   L   S+P YDIL LL+ LEG+N+    L +    + F EG++  LD+
Sbjct: 1244 FLSYLFTHRLALRLKGSSHPPYDILFLLKSLEGMNRFLFHLISLERINAFGEGRLENLDD 1303

Query: 1371 LGGIGGKVPHEEFVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQY 1430
            L      VPH EFV+SKLT KL +Q++D+ A+ +  LP W   L  +CP LF FE + +Y
Sbjct: 1304 LRVQVRPVPHSEFVSSKLTEKLEQQLRDSFAVSTCGLPPWFNDLMDSCPCLFSFEAKSKY 1363

Query: 1431 FYSTAFGLSRALYRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMY 1490
            F   AFG S+ +    Q L +  +    E     G L R+K    R  IL+SA K+ME+Y
Sbjct: 1364 FRLAAFG-SQKIRHHPQHLSSSNVH--GEARPVTGSLPRKKFLACRENILESAAKMMELY 1423

Query: 1491 SNQKAVLEVEYFGEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRK 1550
             NQK V+EVEY  EVGTGLGPTLEFYTL+S   Q   LGMWR++    S   G      K
Sbjct: 1424 GNQKVVIEVEYSEEVGTGLGPTLEFYTLVSRAFQNPDLGMWRNDC---SFIVG------K 1483

Query: 1551 PKDGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQD 1610
            P + S          ++ S  GLFPRPW   + +SD      V++ F L+G V+AKALQD
Sbjct: 1484 PVEHS---------GVLASSSGLFPRPWSGTSTTSD------VLQKFVLLGTVVAKALQD 1502

Query: 1611 GRLLDLPLSTAFYKLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNT 1670
            GR+LDLPLS AFYKL+LGQ+L  +DI   DPEL KTL ELQALV RK+      GD+   
Sbjct: 1544 GRVLDLPLSKAFYKLILGQELSSFDIHFVDPELCKTLVELQALVRRKKLFAEAHGDSGAA 1502

Query: 1671 ISNLSFRGIPVEDLCLDFTVPGYPDYVLRP--GDETVNIHNLEEYISLVLDATVKTGIMR 1730
              +LSF G  +EDLCL+F +PGY DY L P   ++ VN+ NLEEYI  +++ATV  GI +
Sbjct: 1604 KCDLSFHGTKIEDLCLEFALPGYTDYDLAPYSANDMVNLDNLEEYIKGIVNATVCNGIQK 1502

Query: 1731 QMEAFTTGFNQVFDITALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIV 1790
            Q+EAF +GFNQVF I  LRIF   EL+ +LCG  +L+  + ++DHIKFDHGYT+ SP + 
Sbjct: 1664 QVEAFRSGFNQVFSIEHLRIFNEEELETMLCGECDLFSMNEVLDHIKFDHGYTSSSPPVE 1502

Query: 1791 NFLEIMGELTPEQQRGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVS 1850
              L+I+ E   EQQR F QFVTG+PRLP GGLA L+P+LTIVRKH              S
Sbjct: 1724 YLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRKHG-------------S 1502

Query: 1851 ESADDDLPSVMTCANYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            +S+D DLPSVMTCANYLKLPPYS+KE M +KL+YAI EGQGSF LS
Sbjct: 1784 DSSDTDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502

BLAST of Carg16175 vs. ExPASy Swiss-Prot
Match: E1B7Q7 (E3 ubiquitin-protein ligase TRIP12 OS=Bos taurus OX=9913 GN=TRIP12 PE=2 SV=2)

HSP 1 Score: 550.8 bits (1418), Expect = 5.9e-155
Identity = 565/2026 (27.89%), Postives = 867/2026 (42.79%), Query Frame = 0

Query: 13   SAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEPSATSTPMDSTNE 72
            S+   +S  GP+   +K A L  +      +    + +  RSTR +    T +   ++  
Sbjct: 282  SSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQK---TTGSCASASRR 341

Query: 73   SSGSRRRG----KNSDKENSDKGKEK---EHEVRIGDRERNAEQSFGLNIEGSGGGDDDD 132
             SG  +RG    +  +K    +G ++       R  +  + A  + G+   G    DD +
Sbjct: 342  GSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAGAVGMTTSGESESDDSE 401

Query: 133  NDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKKILSGL 192
                  MG LQ  L  A      L   LG  +  L   +  + ASS  Q     ++L GL
Sbjct: 402  ------MGRLQA-LLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQ-----QLLQGL 461

Query: 193  RADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLLAARAL 252
            +A  +E +Q++A+ ++C++L +G EE+L  F V S VP L+ LL  E N DIM  A RAL
Sbjct: 462  QA-SDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRAL 521

Query: 253  THLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGAL 312
            T++ + LP S A VV   A+  F  +L  I+ +D+AEQ+L AL+ +S+ H  A L+AG L
Sbjct: 522  TYMMEALPRSSAVVV--DAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGL 581

Query: 313  MAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKVLEHAS 372
               L YL+FFS   QR AL+ AAN C+ +  D   FV +++PLLT  L + D K +E   
Sbjct: 582  ADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTC 641

Query: 373  VCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIRLLSTC 432
            +C  R+ + F    + L ++ +  L+T    L+  +        LSS  +  ++R+ S  
Sbjct: 642  LCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVT-----PPILSSGMFIMVVRMFSLM 701

Query: 433  ASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANELLPPL 492
             S        L+   I+  L  +L G+   +       + R  ++++E+ +L  EL+P L
Sbjct: 702  CSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCL 761

Query: 493  PQGTISFPPNYNLFVKGPVIKK---PSTSGSV---------------------------- 552
            P+          +F    ++KK    +T G++                            
Sbjct: 762  PK--------EGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDT 821

Query: 553  -------KEEDP-----TDSAPEM---SAREKLLKDQPELLHQFGMDLLPILIQIYGSSV 612
                   ++ +P     T    E+    AR +L+K+ PEL   F   L  +L ++Y SS 
Sbjct: 822  GTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSA 881

Query: 613  NGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVLAWKDPHILIPSLQIAEILM 672
               VRHKCL  I +++YF+  E+++ +L    ++S +A +L+ +D  I++ +LQ+AEILM
Sbjct: 882  GPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILM 941

Query: 673  EKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKDNNSASGASSRTRRYRRRSG 732
            +KLP+ FS  F REGV++ V  L  + +  T+   A      +  S  S  +      + 
Sbjct: 942  QKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATN 1001

Query: 733  NMTSEGSSLDENKNPVSGGSVPQGSV-------EVPSINSNLRTSVSACANAFKTKYFPL 792
                 GS   ++    S    PQG +        +P   S  R   S   +  K      
Sbjct: 1002 ASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSR-RPKYSPPRDDDKVDNQAK 1061

Query: 793  DPGDVEVGVTDDLLRLK-----NLCAKLNAGIDVQKSKSKGKSKASGSCIDDIITNKDQY 852
             P   +   +  L  L       L A+ N+  +++ +++ G S  + +   D I+N  + 
Sbjct: 1062 SPTTTQSPKSSFLASLNPKTWGRLSAQSNSN-NIEPARTAGVSGLARAASKDTISNNREK 1121

Query: 853  LTGVI--------------------------------------------SEMLVELG--- 912
            + G I                                            +E LVE+    
Sbjct: 1122 IKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIV 1181

Query: 913  KDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPKIRQQVLKRFKSLVSVALSTS 972
             +  VS+FE   SG V  LL Y +          ++E   + +++  R K  + V  S+ 
Sbjct: 1182 SESDVSSFEIQHSGFVKQLLLYLTS---------KSEKDAVSREI--RLKRFLHVFFSSP 1241

Query: 973  I-NEGTV--------APMTVLVQKLQSALSSLERFPVVLSH--SSRSSSGSARLSSGLSV 1032
            +  E  +        AP+  LV K+ + LS +E+FPV +    S   + GS  L+ G   
Sbjct: 1242 LPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQA 1301

Query: 1033 L----SQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQKLSVS 1092
            L    +   K +L R      ++ +    V IDPLA + A+E +L               
Sbjct: 1302 LKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYL--------------- 1361

Query: 1093 GANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTSKGKGK 1152
                                                                   +G G+
Sbjct: 1362 -----------------------------------------------------VVRGYGR 1421

Query: 1153 AILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDSLVIED 1212
                                                                    V ED
Sbjct: 1422 --------------------------------------------------------VRED 1481

Query: 1213 DDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTFGSSSR 1272
            D   +D DDD  D   D+SL                                        
Sbjct: 1482 D---EDSDDDGSDEEIDESL---------------------------------------- 1541

Query: 1273 TATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFGGSNDN 1332
             A    S +  HR                                               
Sbjct: 1542 AAQFLNSGNVRHR----------------------------------------------- 1601

Query: 1333 PKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDIYTITY 1392
              L F  GE  L  ++T+YQAV++  +  ED+ E      N       + +W   +TI Y
Sbjct: 1602 --LQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPL---GRAGIWTKTHTIWY 1661

Query: 1393 Q--RADNQSERAALAGESSSSKSKSTKCVSTSNSNFRFHQ-----------MSLLDSILQ 1452
            +  R D +S +  + G+   +++  TK   TS  N + H             + L+  L 
Sbjct: 1662 KPVREDEESNKDCVGGKRGRAQTAPTK---TSPRNAKKHDELWNDGVCPSVSNPLEVYLI 1721

Query: 1453 GKLPCDLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKV 1512
               P ++   +P+ D++ LLRVL  +++    L    +  +                  +
Sbjct: 1722 PTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEI-----------------I 1781

Query: 1513 PHEEFVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGL 1572
            P  EF+NSKLT K  RQ+QD L + +G++P+W  +L K CPF FPF+TR+  FY TAF  
Sbjct: 1782 PTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDR 1841

Query: 1573 SRALYRLHQQLGADGLGTVNEREGRVG-RLQRQKVRVSRNRILDSAVKVMEMYSNQKAVL 1632
             RA+ RL        +   + ++ RV  RL R+K  V+R  +L  A  VM+   + +A+L
Sbjct: 1842 DRAMQRLLDT--NPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAML 1901

Query: 1633 EVEYFGEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKL 1692
            E++Y  EVGTGLGPTLEFY L+S ELQRA LG+WR          GE+  L  PK   + 
Sbjct: 1902 EIQYENEVGTGLGPTLEFYALVSQELQRADLGLWR----------GEEVTLSNPKGSQE- 1961

Query: 1693 ISDAGNIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLP 1752
                     IQ+  GLF  P+   A     +  +KV   FR +G++MAKA+ D RL+DLP
Sbjct: 1962 -----GTKYIQNLQGLFALPFGRTAKP---AHIAKVKMKFRFLGKLMAKAIMDFRLVDLP 1992

Query: 1753 LSTAFYKLVLGQDLDL--YDILSFDPELGKTLQELQALVCRKQYL----GLVDGDNQNTI 1812
            L   FYK +L Q+  L  +D+   DP + +++  L+ +V +K+ L           Q  +
Sbjct: 2022 LGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYAL 1992

Query: 1813 SNLSFRGIPVEDLCLDFTVPGYPDYVLRPG--DETVNIHNLEEYISLVLDATVKTGIMRQ 1872
              L+  G  VEDL LDFT+PG+P+  L+ G  D  V IHNLEEY+ LV+   +  G+ RQ
Sbjct: 2082 ETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQ 1992

Query: 1873 MEAFTTGFNQVFDITALRIFIPHELDHLLCG-RRELWKADTLVDHIKFDHGYTAKSPAIV 1889
             ++F  GF  VF ++ L+ F P ELD LLCG + + W A TL++  + DHGYT  S A+ 
Sbjct: 2142 FDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVK 1992

BLAST of Carg16175 vs. ExPASy Swiss-Prot
Match: Q14669 (E3 ubiquitin-protein ligase TRIP12 OS=Homo sapiens OX=9606 GN=TRIP12 PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.7e-154
Identity = 564/2017 (27.96%), Postives = 860/2017 (42.64%), Query Frame = 0

Query: 13   SAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEPSATSTPMDSTNE 72
            S+   +S  GP+   +K A L  +      +    + +  RSTR +    T +   ++  
Sbjct: 282  SSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQK---TTGSCASTSRR 341

Query: 73   SSG--------SRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGGGDDD 132
             SG        +RR+ K +D E++ +        R  +  + A  + G+   G    DD 
Sbjct: 342  GSGLGKRGAAEARRQEKMADPESNQEAVNSS-AARTDEAPQGAAGAVGMTTSGESESDDS 401

Query: 133  DNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKKILSG 192
            +      MG LQ  L  A      L   LG  +  L   +  + ASS  Q     ++L G
Sbjct: 402  E------MGRLQA-LLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQ-----QLLQG 461

Query: 193  LRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLLAARA 252
            L+A  +E +Q++A+ ++C++L +G EE+L  F V S VP L+ LL  E N DIM  A RA
Sbjct: 462  LQA-SDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRA 521

Query: 253  LTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGA 312
            LT++ + LP S A VV   A+  F  +L  I+ +D+AEQ+L AL+ +S+ H  A L+AG 
Sbjct: 522  LTYMMEALPRSSAVVV--DAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGG 581

Query: 313  LMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKVLEHA 372
            L   L YL+FFS   QR AL+ AAN C+ +  D   FV +++PLLT  L + D K +E  
Sbjct: 582  LADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVEST 641

Query: 373  SVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIRLLST 432
             +C  R+ + F    + L ++ +  L+T    L+  +        LSS  +  ++R+ S 
Sbjct: 642  CLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVT-----PPILSSGMFIMVVRMFSL 701

Query: 433  CASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANELLPP 492
              S        L+   I+  L  +L G+   +       + R  ++++E+ +L  EL+P 
Sbjct: 702  MCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPC 761

Query: 493  LPQ---------------------------------------GTISFPPNYNLFVKGPVI 552
            LP+                                         I    N +      + 
Sbjct: 762  LPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQ 821

Query: 553  KKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELLHQFGMDLLPILIQIYGSSVNGPVRH 612
            +KP+   +      ++S  +  AR +L+K+ PEL   F   L  +L ++Y SS    VRH
Sbjct: 822  RKPNPLANSNTSGYSESKKD-DARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRH 881

Query: 613  KCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVLAWKDPHILIPSLQIAEILMEKLPET 672
            KCL  I +++YF+  E+++ +L    ++S +A +L+ +D  I++ +LQ+AEILM+KLP+ 
Sbjct: 882  KCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDI 941

Query: 673  FSKMFLREGVVYAVDQLILANNQNTATQVASVEKDNNSASGASSRTRRYRRRSGNMTSEG 732
            FS  F REGV++ V  L  + +  T+   A      +  S  S  +      +      G
Sbjct: 942  FSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLG 1001

Query: 733  SSLDENKNPVSGGSVPQGSVEVPSINSNL------RTSVSACANAFKTKYFPLDPGDVEV 792
            S   ++    S    PQG +        L      R   S   +  K       P   + 
Sbjct: 1002 SPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQS 1061

Query: 793  GVTDDLLRLK-----NLCAKLNAGIDVQKSKSKGKSKASGSCIDDIITNKDQYLTGVI-- 852
              +  L  L       L  + N+  +++ +++ G S  + +   D I+N  + + G I  
Sbjct: 1062 PKSSFLASLNPKTWGRLSTQSNSN-NIEPARTAGGSGLARAASKDTISNNREKIKGWIKE 1121

Query: 853  ------------------------------------------SEMLVELG---KDDGVST 912
                                                      +E LVE+     +  VS+
Sbjct: 1122 QAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSS 1181

Query: 913  FEFIGSGVVGVLLDYFSCGYFSKGRILEAELPKIRQQV-LKRFKSL-VSVALSTSINEGT 972
            FE   SG V  LL Y +          ++E   + +++ LKRF  +  S  L      G 
Sbjct: 1182 FEIQHSGFVKQLLLYLTS---------KSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGR 1241

Query: 973  V-----APMTVLVQKLQSALSSLERFPVVLSH--SSRSSSGSARLSSGLSVL----SQPF 1032
            V     AP+  LV K+ + LS +E+FPV +    S   + GS  L+ G   L    +   
Sbjct: 1242 VEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQL 1301

Query: 1033 KLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQKLSVSGANSDSGTT 1092
            K +L R      ++ +    V IDPLA + A+E +L                        
Sbjct: 1302 KCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYL------------------------ 1361

Query: 1093 PPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTSKGKGKAILKPAWEE 1152
                                                          +G G+         
Sbjct: 1362 --------------------------------------------VVRGYGR--------- 1421

Query: 1153 KRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDSLVIEDDDISDDEDD 1212
                                                           V EDD   +D DD
Sbjct: 1422 -----------------------------------------------VREDD---EDSDD 1481

Query: 1213 DHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTFGSSSRTATIRGSNS 1272
            D  D   D+SL                                         A    S +
Sbjct: 1482 DGSDEEIDESL----------------------------------------AAQFLNSGN 1541

Query: 1273 PDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFGGSNDNPKLLFSSGE 1332
              HR                                                 L F  GE
Sbjct: 1542 VRHR-------------------------------------------------LQFYIGE 1601

Query: 1333 KQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDIYTITYQ--RADNQS 1392
              L  ++T+YQAV++  +  ED+ E      N       + +W   +TI Y+  R D +S
Sbjct: 1602 HLLPYNMTVYQAVRQFSIQAEDERESTDDESNPL---GRAGIWTKTHTIWYKPVREDEES 1661

Query: 1393 ERAALAGESSSSKSKSTKCVSTSNSNFRFHQ-----------MSLLDSILQGKLPCDLDK 1452
             +  + G+   +++  TK   TS  N + H             + L+  L    P ++  
Sbjct: 1662 NKDCVGGKRGRAQTAPTK---TSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITF 1721

Query: 1453 SNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFVNSK 1512
             +P+ D++ LLRVL  +++    L    +  +                  +P  EF+NSK
Sbjct: 1722 EDPSLDVILLLRVLHAISRYWYYLYDNAMCKEI-----------------IPTSEFINSK 1781

Query: 1513 LTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYRLHQ 1572
            LT K  RQ+QD L + +G++P+W  +L K CPF FPF+TR+  FY TAF   RA+ RL  
Sbjct: 1782 LTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLD 1841

Query: 1573 QLGADGLGTVNEREGRVG-RLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGEVG 1632
                  +   + ++ RV  RL R+K  V+R  +L  A  VM+   + +A+LE++Y  EVG
Sbjct: 1842 T--NPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVG 1901

Query: 1633 TGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNIDI 1692
            TGLGPTLEFY L+S ELQRA LG+WR          GE+  L  PK   +          
Sbjct: 1902 TGLGPTLEFYALVSQELQRADLGLWR----------GEEVTLSNPKGSQE------GTKY 1961

Query: 1693 IQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYKLV 1752
            IQ+  GLF  P+   A     +  +KV   FR +G++MAKA+ D RL+DLPL   FYK +
Sbjct: 1962 IQNLQGLFALPFGRTAKP---AHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWM 1992

Query: 1753 LGQDLDL--YDILSFDPELGKTLQELQALVCRKQYL----GLVDGDNQNTISNLSFRGIP 1812
            L Q+  L  +D+   DP + +++  L+ +V +K+ L           Q  +  L+  G  
Sbjct: 2022 LRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCS 1992

Query: 1813 VEDLCLDFTVPGYPDYVLRPG--DETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFN 1872
            VEDL LDFT+PG+P+  L+ G  D  V IHNLEEY+ LV+   +  G+ RQ ++F  GF 
Sbjct: 2082 VEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFE 1992

Query: 1873 QVFDITALRIFIPHELDHLLCG-RRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEL 1889
             VF ++ L+ F P ELD LLCG + + W A TL++  + DHGYT  S A+    EI+   
Sbjct: 2142 SVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSF 1992

BLAST of Carg16175 vs. ExPASy Swiss-Prot
Match: F1LP64 (E3 ubiquitin-protein ligase TRIP12 OS=Rattus norvegicus OX=10116 GN=Trip12 PE=1 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 8.0e-152
Identity = 573/2054 (27.90%), Postives = 868/2054 (42.26%), Query Frame = 0

Query: 16   PSSSSSGPTTRSSKRARLSGT-FSSNLAASTLSISTRSRSTRNQEPS-ATSTPMDSTNES 75
            P +  S P +  S+ ++   +   + LA+   S   RS S   + PS   ST   +T   
Sbjct: 273  PKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSC 332

Query: 76   SGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAE---------------QSFGLNIEGS 135
            + + RRG    K  + + + +E   ++ D E N E                +   ++ G+
Sbjct: 333  ASTSRRGSGLGKRGAAEARRQE---KMADPEGNQETVNSSAARTDEAPQGAAASSSVAGA 392

Query: 136  GG----GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQ 195
             G    G+ + +DSE  MG LQ  L  A      L   LG  +  L   +  + ASS  Q
Sbjct: 393  VGMTTSGESESDDSE--MGRLQA-LLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQ 452

Query: 196  RGRLKKILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESN 255
                 ++L GL+A  +E +Q++A+ ++C++L +G EE+L  F V S VP L+ LL  E N
Sbjct: 453  -----QLLQGLQA-SDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHN 512

Query: 256  PDIMLLAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQE 315
             DIM  A RALT++ + LP S A VV   A+  F  +L  I+ +D+AEQ+L AL+ +S+ 
Sbjct: 513  FDIMNHACRALTYMMEALPRSSAVVV--DAIPVFLEKLQVIQCIDVAEQALTALEMLSRR 572

Query: 316  HPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQ 375
            H  A L+AG L   L YL+FFS   QR AL+ AAN C+ +  D   FV +++PLLT  L 
Sbjct: 573  HSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLT 632

Query: 376  YHDAKVLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSAT 435
            + D K +E   +C  R+ + F    + L ++ +  L+T    L+  +        LSS  
Sbjct: 633  HQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVT-----PPILSSGM 692

Query: 436  YTGLIRLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEI 495
            +  ++R+ S   S        L+   I+  L  +L G+   +       + R  ++++E+
Sbjct: 693  FIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYEL 752

Query: 496  VNLANELLPPLPQG---------------------------------------------- 555
             +L  EL+P LP+                                               
Sbjct: 753  TSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAH 812

Query: 556  ----------------TISFPP----NYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSA 615
                            TI F      N +      + +KP+   +      +D   +  A
Sbjct: 813  QVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSDLKKD-DA 872

Query: 616  REKLLKDQPELLHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLN 675
            R +L+K+ PEL   F   L  +L ++Y SS    VRHKCL  I +++YF+  E+++ +L 
Sbjct: 873  RAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLK 932

Query: 676  VTNIASFLAGVLAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQ 735
               ++S +A +L+ +D  I++ +LQ+AEILM+KLP+ FS  F REGV++ V  L  + + 
Sbjct: 933  NHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESL 992

Query: 736  NTATQVASVEKDNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVP 795
             T+   A      +  S   + +      +      GS   ++    S    PQG +   
Sbjct: 993  LTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDV 1052

Query: 796  SINSNL------RTSVSACANAFKTKYFPLDPGDVEVGVTDDLLRLK-----NLCAKLNA 855
                 L      R   S   +  K       P   +   +  L  L       L A+ N+
Sbjct: 1053 LKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNS 1112

Query: 856  GIDVQKSKSKGKSKASGSCIDDIITNKDQYLTGVI------------------------- 915
              +++ +++ G S  + +   D I+N  + + G I                         
Sbjct: 1113 N-NIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALN 1172

Query: 916  -------------------SEMLVELG---KDDGVSTFEFIGSGVVGVLLDYFSCGYFSK 975
                               +E LVE+     +  VS+FE   SG V  LL Y +      
Sbjct: 1173 VLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTS----- 1232

Query: 976  GRILEAELPKIRQQV-LKRFKSL-VSVALSTSINEGTV-----APMTVLVQKLQSALSSL 1035
                ++E   + +++ LKRF  +  S  L      G V     AP+  LV K+ + LS +
Sbjct: 1233 ----KSEKDAVSREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQM 1292

Query: 1036 ERFPVVLSH--SSRSSSGSARLSSGLSVL----SQPFKLRLCRAHGEKLLRDYSSNIVLI 1095
            E+FPV +    S   S GS  L+ G   L    +   K +L R      ++ +    V I
Sbjct: 1293 EQFPVKVHDFPSGNGSGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKI 1352

Query: 1096 DPLASLAAVEEFLWPRVQRSEGQKLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYST 1155
            DPLA + A+E +L                                               
Sbjct: 1353 DPLALVQAIERYL----------------------------------------------- 1412

Query: 1156 RSRSSMTIGERAGKELSQDKSTSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMK 1215
                                   +G G+                                
Sbjct: 1413 ---------------------VVRGYGR-------------------------------- 1472

Query: 1216 PVNGETTSEDEELDLSSIQIDDSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVK 1275
                                    V EDD   +D DDD  D   D+SL            
Sbjct: 1473 ------------------------VREDD---EDSDDDGSDEEIDESL------------ 1532

Query: 1276 LGDTAEDGDAGPATSDSQIHSTFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMA 1335
                                         A    S +  HR                   
Sbjct: 1533 ----------------------------AAQFLNSGNVRHR------------------- 1592

Query: 1336 GLGPANGRGFRGGRDPQGHPLFGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDD 1395
                                          L F  GE  L  ++T+YQAV++  V  ED+
Sbjct: 1593 ------------------------------LQFYIGEHLLPYNMTVYQAVRQFSVQAEDE 1652

Query: 1396 DERFAGAGNDFLSNDGSSLWGDIYTITYQ--RADNQSERAALAGESSSSKSKSTKCVSTS 1455
             E      N       + +W   +TI Y+  R D +S +  + G+   +++  TK   TS
Sbjct: 1653 RESTDDESNPL---GRAGIWTKTHTIWYKPVREDEESSKDCVGGKRGRAQTAPTK---TS 1712

Query: 1456 NSNFRFHQ-----------MSLLDSILQGKLPCDLDKSNPTYDILSLLRVLEGLNQLAPR 1515
              N + H             + L+  L    P ++   +P+ D++ LLRVL  +++    
Sbjct: 1713 PRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYY 1772

Query: 1516 LRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFVNSKLTPKLTRQIQDALALCSGSLPSW 1575
            L    +  +                  +P  EF+NSKLT K  RQ+QD L + +G++P+W
Sbjct: 1773 LYDNAMCKEI-----------------IPTSEFINSKLTAKANRQLQDPLVIMTGNIPTW 1832

Query: 1576 CYQLTKACPFLFPFETRRQYFYSTAFGLSRALYRLHQQLGADGLGTVNEREGRVG-RLQR 1635
              +L K CPF FPF+TR+  FY TAF   RA+ RL        +   + ++ RV  RL R
Sbjct: 1833 LTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDT--NPEINQSDSQDSRVAPRLDR 1892

Query: 1636 QKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGEVGTGLGPTLEFYTLLSHELQRAGLG 1695
            +K  V+R  +L  A  VM+   + +A+LE++Y  EVGTGLGPTLEFY L+S ELQRA LG
Sbjct: 1893 KKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLG 1952

Query: 1696 MWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDGSQ 1755
            +WR          GE+  L  PK   +          IQ+  GLF  P+   A     + 
Sbjct: 1953 LWR----------GEEVTLSNPKGSQE------GTKYIQNLQGLFALPFGRTAKP---AH 2012

Query: 1756 FSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYKLVLGQDLDL--YDILSFDPELGKTL 1815
             +KV   FR +G++MAKA+ D RL+DLPL   FYK +L Q+  L  +D+   DP + +++
Sbjct: 2013 IAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSV 2025

Query: 1816 QELQALVCRKQYL----GLVDGDNQNTISNLSFRGIPVEDLCLDFTVPGYPDYVLRPG-- 1875
              L+ +V +K+ L           Q  +  L+  G  VEDL LDFT+PG+P+  L+ G  
Sbjct: 2073 YHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGK 2025

Query: 1876 DETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDITALRIFIPHELDHLLCG- 1889
            D  V IHNLEEY+ LV+   +  G+ RQ ++F  GF  VF ++ L+ F P ELD LLCG 
Sbjct: 2133 DIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGS 2025

BLAST of Carg16175 vs. ExPASy TrEMBL
Match: A0A6J1FT18 (E3 ubiquitin-protein ligase UPL3-like OS=Cucurbita moschata OX=3662 GN=LOC111448208 PE=4 SV=1)

HSP 1 Score: 3577.7 bits (9276), Expect = 0.0e+00
Identity = 1884/1890 (99.68%), Postives = 1886/1890 (99.79%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60
            METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60

Query: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120
            SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG
Sbjct: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120

Query: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180
            GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK
Sbjct: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180

Query: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240
            ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL
Sbjct: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240

Query: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300
            AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL
Sbjct: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300

Query: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360
            RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV
Sbjct: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360

Query: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420
            LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR
Sbjct: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420

Query: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480
            LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE
Sbjct: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480

Query: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540
            LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL
Sbjct: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540

Query: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600
            HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL
Sbjct: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600

Query: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660
            AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD
Sbjct: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660

Query: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720
            NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA
Sbjct: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720

Query: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780
            CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI
Sbjct: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780

Query: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840
            ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK
Sbjct: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840

Query: 841  IRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900
            IRQQVLKRFKS VSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS
Sbjct: 841  IRQQVLKRFKSFVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900

Query: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960
            ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ
Sbjct: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960

Query: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020
            KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS
Sbjct: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020

Query: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080
            KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS
Sbjct: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080

Query: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF 1140
            LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF
Sbjct: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF 1140

Query: 1141 GSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200
            GSSSRTAT+RGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG
Sbjct: 1141 GSSSRTATVRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200

Query: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260
            GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI
Sbjct: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260

Query: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLPCD 1320
            YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN   +FHQMSLLDSILQGKLPCD
Sbjct: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSNSESQFHQMSLLDSILQGKLPCD 1320

Query: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380
            LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV
Sbjct: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380

Query: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440
            NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR
Sbjct: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440

Query: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500
            LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE
Sbjct: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500

Query: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560
            VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI
Sbjct: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560

Query: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620
            DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK
Sbjct: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620

Query: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680
            LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL
Sbjct: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680

Query: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740
            CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT
Sbjct: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740

Query: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG 1800
            ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG
Sbjct: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG 1800

Query: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY 1860
            FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY
Sbjct: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY 1860

Query: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            LKLPPYSTKEIMYKKLMYAINEGQGSFDLS
Sbjct: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1890

BLAST of Carg16175 vs. ExPASy TrEMBL
Match: A0A6J1JIB3 (E3 ubiquitin-protein ligase UPL3-like OS=Cucurbita maxima OX=3661 GN=LOC111484604 PE=4 SV=1)

HSP 1 Score: 3539.6 bits (9177), Expect = 0.0e+00
Identity = 1867/1890 (98.78%), Postives = 1874/1890 (99.15%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60
            METRSRKRAEASSAAPSSSSSGPTTRS+KRARLSGTFSSNLAASTLSISTRSRSTRNQEP
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPTTRSTKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60

Query: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120
            SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG
Sbjct: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120

Query: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180
            GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK
Sbjct: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180

Query: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240
            ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL
Sbjct: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240

Query: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300
            AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL
Sbjct: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300

Query: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360
            RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV
Sbjct: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360

Query: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420
            LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR
Sbjct: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420

Query: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480
            LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE
Sbjct: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480

Query: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540
            LLPPLPQGTISFP NYNLFVKGPVIKKPSTSGSVKEEDPTDS PE+SAREKLLKDQPELL
Sbjct: 481  LLPPLPQGTISFPSNYNLFVKGPVIKKPSTSGSVKEEDPTDSVPEVSAREKLLKDQPELL 540

Query: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600
            HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL
Sbjct: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600

Query: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660
            AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNT+TQVASVEKD
Sbjct: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTSTQVASVEKD 660

Query: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720
            NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA
Sbjct: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720

Query: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780
            CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGS IDDI
Sbjct: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSRIDDI 780

Query: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840
            ITNK+QYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK
Sbjct: 781  ITNKEQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840

Query: 841  IRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900
            IRQQVLKRFKS VSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS
Sbjct: 841  IRQQVLKRFKSFVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900

Query: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960
            ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ
Sbjct: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSEGQ 960

Query: 961  KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020
            KLSVSGANSDSGT PPGTVAPSGLKSTPS TTCRYSTRSRSSMTIGERAGKELSQDKSTS
Sbjct: 961  KLSVSGANSDSGTMPPGTVAPSGLKSTPSPTTCRYSTRSRSSMTIGERAGKELSQDKSTS 1020

Query: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080
            KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS
Sbjct: 1021 KGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDDS 1080

Query: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHSTF 1140
            LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQI STF
Sbjct: 1081 LVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIPSTF 1140

Query: 1141 GSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200
            GSSSRTAT RGSNSPDHRGGNSFSARG MTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG
Sbjct: 1141 GSSSRTATARGSNSPDHRGGNSFSARGGMTFAAAAMAGLGPANGRGFRGGRDPQGHPLFG 1200

Query: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260
            GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI
Sbjct: 1201 GSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGDI 1260

Query: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLPCD 1320
            YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN   +FHQMSLLDSILQGKLPCD
Sbjct: 1261 YTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSNSESQFHQMSLLDSILQGKLPCD 1320

Query: 1321 LDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380
            LDKSN TYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV
Sbjct: 1321 LDKSNSTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEFV 1380

Query: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440
            NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR
Sbjct: 1381 NSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALYR 1440

Query: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500
            LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE
Sbjct: 1441 LHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFGE 1500

Query: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560
            VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI
Sbjct: 1501 VGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNI 1560

Query: 1561 DIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620
            DIIQSPLGLFP+PW PNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK
Sbjct: 1561 DIIQSPLGLFPQPWQPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYK 1620

Query: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680
            LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL
Sbjct: 1621 LVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDL 1680

Query: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740
            CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT
Sbjct: 1681 CLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDIT 1740

Query: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRG 1800
            ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQRG
Sbjct: 1741 ALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQRG 1800

Query: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANY 1860
            FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTA+NAANSATG SESADDDLPSVMTCANY
Sbjct: 1801 FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTASNAANSATGASESADDDLPSVMTCANY 1860

Query: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            LKLPPYSTKEIMYKKLMYAINEGQGSFDLS
Sbjct: 1861 LKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1890

BLAST of Carg16175 vs. ExPASy TrEMBL
Match: A0A1S3C6R5 (E3 ubiquitin-protein ligase UPL3 OS=Cucumis melo OX=3656 GN=LOC103497327 PE=4 SV=1)

HSP 1 Score: 3369.3 bits (8735), Expect = 0.0e+00
Identity = 1779/1892 (94.03%), Postives = 1821/1892 (96.25%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNL-AASTLSISTRSRSTRNQE 60
            METRSRKRAEASSAAPSSSSSGPTTRS+KRARLS T SSNL AASTLSISTRSRSTR QE
Sbjct: 1    METRSRKRAEASSAAPSSSSSGPTTRSTKRARLSATSSSNLAAASTLSISTRSRSTRTQE 60

Query: 61   PSATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSG 120
            PSAT+TPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNA+QSFGLNIEGSG
Sbjct: 61   PSATTTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNADQSFGLNIEGSG 120

Query: 121  GGDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK 180
            GG+DDDNDSEGGMGVLQ NLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK
Sbjct: 121  GGEDDDNDSEGGMGVLQHNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLK 180

Query: 181  KILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML 240
            KILSGLRADGEEG+QVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML
Sbjct: 181  KILSGLRADGEEGKQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIML 240

Query: 241  LAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTAC 300
            LAARALTHLCD LPSSCAAVVHYGAV CFCARLLTIEYMDLAEQSLQALKKISQEHPTAC
Sbjct: 241  LAARALTHLCDVLPSSCAAVVHYGAVPCFCARLLTIEYMDLAEQSLQALKKISQEHPTAC 300

Query: 301  LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK 360
            LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK
Sbjct: 301  LRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAK 360

Query: 361  VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLI 420
            VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTL SATYTGLI
Sbjct: 361  VLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLGSATYTGLI 420

Query: 421  RLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLAN 480
            RLLSTCASGSALG KTLLLLGISGILKDILSGSGVSTN+SVSPALNRPQEQIFEIVNLAN
Sbjct: 421  RLLSTCASGSALGAKTLLLLGISGILKDILSGSGVSTNASVSPALNRPQEQIFEIVNLAN 480

Query: 481  ELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPEL 540
            ELLPPLPQGTISFPPN+N+FVKGPVIKKPSTSGSVKEEDPTDSAPE+SAREKLLKDQPEL
Sbjct: 481  ELLPPLPQGTISFPPNFNMFVKGPVIKKPSTSGSVKEEDPTDSAPEVSAREKLLKDQPEL 540

Query: 541  LHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV 600
            L QFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV
Sbjct: 541  LLQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGV 600

Query: 601  LAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEK 660
            LAWKDPHILIP+LQIAEILMEKL ETFSKMFLREGVVYAVDQLILANNQNT++Q ASVEK
Sbjct: 601  LAWKDPHILIPALQIAEILMEKLHETFSKMFLREGVVYAVDQLILANNQNTSSQAASVEK 660

Query: 661  DNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVS 720
            D+ SASG SSRTRRYRRRSG M S+GSSLDENKN VSG  VPQGSVEVPSINSNLR+SVS
Sbjct: 661  DSTSASGTSSRTRRYRRRSGTMNSDGSSLDENKNSVSGSGVPQGSVEVPSINSNLRSSVS 720

Query: 721  ACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDD 780
            +CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLC KLNAGID QKSKSKGK KASGS +DD
Sbjct: 721  SCANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCFKLNAGIDDQKSKSKGKLKASGSRLDD 780

Query: 781  IITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELP 840
            IITNK++YLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLL+YFSCGYFSKGRIL+ ELP
Sbjct: 781  IITNKEEYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLNYFSCGYFSKGRILDVELP 840

Query: 841  KIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG 900
            K+RQQVLKRFKS +SVAL   INEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG
Sbjct: 841  KLRQQVLKRFKSFISVALPARINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSG 900

Query: 901  SARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSE- 960
            SARLSSGLSVLSQPFKLRLCRA GEK LRDYSSNIVLIDPLASLAAVEEFLWPRVQ+SE 
Sbjct: 901  SARLSSGLSVLSQPFKLRLCRAQGEKSLRDYSSNIVLIDPLASLAAVEEFLWPRVQKSES 960

Query: 961  GQKLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKS 1020
            GQK S SGANSDSGTTP G VAPSGL STPSST  RYSTRSRSSMTIGERAGKE SQ+KS
Sbjct: 961  GQKPSASGANSDSGTTPSGNVAPSGLNSTPSSTARRYSTRSRSSMTIGERAGKESSQEKS 1020

Query: 1021 TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQID 1080
            TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQID
Sbjct: 1021 TSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQID 1080

Query: 1081 DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHS 1140
            DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKL DT EDGDAGPATSD QIHS
Sbjct: 1081 DSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLSDTVEDGDAGPATSDGQIHS 1140

Query: 1141 TFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPL 1200
            TFGSSSR AT+RGS+SPDHR GNSFS+RG M+FAAAAMAGLGPANGRGFRGGRDPQG PL
Sbjct: 1141 TFGSSSRAATVRGSSSPDHRSGNSFSSRGGMSFAAAAMAGLGPANGRGFRGGRDPQGRPL 1200

Query: 1201 FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWG 1260
            FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAG+DFLSNDGSSLWG
Sbjct: 1201 FGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGSDFLSNDGSSLWG 1260

Query: 1261 DIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSN--FRFHQMSLLDSILQGKLP 1320
            DIYTITYQRADNQSERAALAGESSSSKSKSTKCVS SNSN   +FHQMSLLDSILQGKLP
Sbjct: 1261 DIYTITYQRADNQSERAALAGESSSSKSKSTKCVSASNSNSESQFHQMSLLDSILQGKLP 1320

Query: 1321 CDLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEE 1380
            CD DKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKIT LDELGG+GGKVPHEE
Sbjct: 1321 CDFDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITALDELGGVGGKVPHEE 1380

Query: 1381 FVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL 1440
            F+N+KLTPKL RQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL
Sbjct: 1381 FINNKLTPKLARQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRAL 1440

Query: 1441 YRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYF 1500
            YRLHQQ GADG+GTVNEREGRVGRLQRQKVRVSRNRILDSA KVMEMYS+QKAVLEVEYF
Sbjct: 1441 YRLHQQQGADGIGTVNEREGRVGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYF 1500

Query: 1501 GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAG 1560
            GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQES DSGEDGQ RK K GS+L SDA 
Sbjct: 1501 GEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESTDSGEDGQARKLKGGSRLTSDAA 1560

Query: 1561 NIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF 1620
            NIDIIQSPLGLFPRPWPPNADSSDGSQ SKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF
Sbjct: 1561 NIDIIQSPLGLFPRPWPPNADSSDGSQLSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAF 1620

Query: 1621 YKLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVE 1680
            YKLVLGQDLDL+DILSFD ELGKTLQELQALVCRKQYL  ++GDNQNTISNL+FRGIPVE
Sbjct: 1621 YKLVLGQDLDLHDILSFDAELGKTLQELQALVCRKQYLESINGDNQNTISNLTFRGIPVE 1680

Query: 1681 DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFD 1740
            DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFT GFNQVFD
Sbjct: 1681 DLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTAGFNQVFD 1740

Query: 1741 ITALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQ 1800
            ITAL IFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQ
Sbjct: 1741 ITALHIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQ 1800

Query: 1801 RGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCA 1860
            R FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATN ANSATG SESADDDLPSVMTCA
Sbjct: 1801 RAFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNTANSATGASESADDDLPSVMTCA 1860

Query: 1861 NYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            NYLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1861 NYLKLPPYSTKEIMYKKLIYAINEGQGSFDLS 1892

BLAST of Carg16175 vs. ExPASy TrEMBL
Match: A0A6J1CXA0 (E3 ubiquitin-protein ligase UPL3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015428 PE=4 SV=1)

HSP 1 Score: 3367.4 bits (8730), Expect = 0.0e+00
Identity = 1780/1891 (94.13%), Postives = 1830/1891 (96.77%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60
            METRSRKRAEASSAAP SSSSGPTTRS+KRARLS T S NLAASTL+ISTRSRSTR QEP
Sbjct: 1    METRSRKRAEASSAAP-SSSSGPTTRSNKRARLSATSSPNLAASTLAISTRSRSTRTQEP 60

Query: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120
            SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG
Sbjct: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120

Query: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180
            G+DDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK
Sbjct: 121  GEDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180

Query: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240
            ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL
Sbjct: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240

Query: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300
            AARALTHLCD LPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL
Sbjct: 241  AARALTHLCDVLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300

Query: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360
            RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV
Sbjct: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360

Query: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420
            LEHASVCLTRI+EAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQS+LSSATYTGLIR
Sbjct: 361  LEHASVCLTRISEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSSLSSATYTGLIR 420

Query: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480
            LLSTCASGSALG KTLLLLGISGILKDILSGSG+STN+SVSPALNRPQEQIFEIVNLANE
Sbjct: 421  LLSTCASGSALGAKTLLLLGISGILKDILSGSGMSTNASVSPALNRPQEQIFEIVNLANE 480

Query: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540
            LLPPLPQGTISFPPN+N+FVKGPVIKKPSTSGSVKEEDPTD+ PE+SAREKLLKDQPELL
Sbjct: 481  LLPPLPQGTISFPPNFNMFVKGPVIKKPSTSGSVKEEDPTDNVPEVSAREKLLKDQPELL 540

Query: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600
            HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL
Sbjct: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600

Query: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660
            AWKDPHILIP+LQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTA+Q ASVEKD
Sbjct: 601  AWKDPHILIPALQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTASQAASVEKD 660

Query: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720
            NNSASG SSRTRRYRRRSGNM S+GSSLDENKNPV   SVPQGSVEVPSINSNLRT+VSA
Sbjct: 661  NNSASGTSSRTRRYRRRSGNMNSDGSSLDENKNPV---SVPQGSVEVPSINSNLRTAVSA 720

Query: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780
            CANAFKTKYFPLD GDVEVGVTDDLLRLKNLC KLNAGID QK+KSKGK KASGS +DDI
Sbjct: 721  CANAFKTKYFPLDQGDVEVGVTDDLLRLKNLCVKLNAGIDDQKTKSKGKLKASGSRLDDI 780

Query: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840
            ITNK++YLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLL+YFSCGYFSKGRI EAELPK
Sbjct: 781  ITNKEEYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLNYFSCGYFSKGRISEAELPK 840

Query: 841  IRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900
            +RQQVLKRFKS VSVAL TSINEGTVA M VLVQKLQSALSSLERFPVVLSHSSRSS+GS
Sbjct: 841  LRQQVLKRFKSFVSVALPTSINEGTVAHMRVLVQKLQSALSSLERFPVVLSHSSRSSNGS 900

Query: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSE-G 960
            ARLSSGLSVLSQPFKLRLCRA G+K LRDYSSNIVLIDPLASLAAVEEFLWPRVQ+SE G
Sbjct: 901  ARLSSGLSVLSQPFKLRLCRAQGDKSLRDYSSNIVLIDPLASLAAVEEFLWPRVQKSESG 960

Query: 961  QKLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKST 1020
            QK S SGANSDSGTTPPGTVAPSGL STPSSTT RYSTRSRSSMTIGER+GKE SQ+KST
Sbjct: 961  QKPSASGANSDSGTTPPGTVAPSGLNSTPSSTTRRYSTRSRSSMTIGERSGKESSQEKST 1020

Query: 1021 SKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDD 1080
            SKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDD
Sbjct: 1021 SKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDD 1080

Query: 1081 SLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHST 1140
            SLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKL D  EDGDAGPATSDSQIHST
Sbjct: 1081 SLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLSDGVEDGDAGPATSDSQIHST 1140

Query: 1141 FGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLF 1200
            FGSSSR AT+RGSNSPDHR GNS+S+RG M+FAAAAMAGLGPANG+ FRGGRDPQG PLF
Sbjct: 1141 FGSSSRAATVRGSNSPDHRSGNSYSSRGGMSFAAAAMAGLGPANGKAFRGGRDPQGRPLF 1200

Query: 1201 GGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGD 1260
            G SNDNPKL+FSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAG+DFLSNDGSSLWGD
Sbjct: 1201 GSSNDNPKLVFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGSDFLSNDGSSLWGD 1260

Query: 1261 IYTITYQRADNQSERAALAGESSSSKSKSTKCVST--SNSNFRFHQMSLLDSILQGKLPC 1320
            IYTITYQRADNQSER ALAGESSSSKSKSTKCVST  SNS  +FHQMSLLDSILQGKLPC
Sbjct: 1261 IYTITYQRADNQSER-ALAGESSSSKSKSTKCVSTSGSNSESQFHQMSLLDSILQGKLPC 1320

Query: 1321 DLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEF 1380
            DLDK+NPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEF
Sbjct: 1321 DLDKTNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEF 1380

Query: 1381 VNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALY 1440
            +NSKLTPKL RQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALY
Sbjct: 1381 INSKLTPKLARQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALY 1440

Query: 1441 RLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFG 1500
            RLHQQ GADGLGTVNEREGRVGRLQRQKVRVSRNRILDSA KVMEMYS+QKAVLEVEYFG
Sbjct: 1441 RLHQQQGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFG 1500

Query: 1501 EVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGN 1560
            EVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESI+S EDGQLRK K GS+LISDAGN
Sbjct: 1501 EVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIESDEDGQLRKAKGGSRLISDAGN 1560

Query: 1561 IDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFY 1620
            IDIIQSPLGLFPRPW P+ADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFY
Sbjct: 1561 IDIIQSPLGLFPRPWSPDADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFY 1620

Query: 1621 KLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVED 1680
            KLVLGQDLDL+DILSFD ELGKTLQELQALVCRKQYL L++GDNQNT+SNLSFRGIPVED
Sbjct: 1621 KLVLGQDLDLHDILSFDAELGKTLQELQALVCRKQYLVLINGDNQNTVSNLSFRGIPVED 1680

Query: 1681 LCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDI 1740
            LCLDFT+PGYP+YVL+PGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFT GFNQVFDI
Sbjct: 1681 LCLDFTLPGYPNYVLKPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTAGFNQVFDI 1740

Query: 1741 TALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQR 1800
            TALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQR
Sbjct: 1741 TALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQR 1800

Query: 1801 GFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCAN 1860
             FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSS+A+NAA S TG SESADDDLPSVMTCAN
Sbjct: 1801 AFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSSASNAATSGTGASESADDDLPSVMTCAN 1860

Query: 1861 YLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            YLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1861 YLKLPPYSTKEIMYKKLIYAINEGQGSFDLS 1886

BLAST of Carg16175 vs. ExPASy TrEMBL
Match: A0A6J1CWG0 (E3 ubiquitin-protein ligase UPL3 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015428 PE=4 SV=1)

HSP 1 Score: 3356.6 bits (8702), Expect = 0.0e+00
Identity = 1777/1891 (93.97%), Postives = 1827/1891 (96.62%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSSSGPTTRSSKRARLSGTFSSNLAASTLSISTRSRSTRNQEP 60
            METRSRKRAEASSAAP SSSSGPTTRS+KRARLS T S NLAASTL+ISTRSRSTR QEP
Sbjct: 1    METRSRKRAEASSAAP-SSSSGPTTRSNKRARLSATSSPNLAASTLAISTRSRSTRTQEP 60

Query: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120
            SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG
Sbjct: 61   SATSTPMDSTNESSGSRRRGKNSDKENSDKGKEKEHEVRIGDRERNAEQSFGLNIEGSGG 120

Query: 121  GDDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180
            G+DDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK
Sbjct: 121  GEDDDNDSEGGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRGRLKK 180

Query: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240
            ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL
Sbjct: 181  ILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPDIMLL 240

Query: 241  AARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300
            AARALTHLCD LPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL
Sbjct: 241  AARALTHLCDVLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHPTACL 300

Query: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360
            RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV
Sbjct: 301  RAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYHDAKV 360

Query: 361  LEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYTGLIR 420
            LEHASVCLTRI+EAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQS+LSSATYTGLIR
Sbjct: 361  LEHASVCLTRISEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSSLSSATYTGLIR 420

Query: 421  LLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVNLANE 480
            LLSTCASGSALG KTLLLLGISGILKDILSGSG+STN+SVSPALNRPQEQIFEIVNLANE
Sbjct: 421  LLSTCASGSALGAKTLLLLGISGILKDILSGSGMSTNASVSPALNRPQEQIFEIVNLANE 480

Query: 481  LLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQPELL 540
            LLPPLPQGTISFPPN+N+FVKGPVIKKPSTSGSVKEEDPTD+ PE+SAREKLLKDQPELL
Sbjct: 481  LLPPLPQGTISFPPNFNMFVKGPVIKKPSTSGSVKEEDPTDNVPEVSAREKLLKDQPELL 540

Query: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600
            HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL
Sbjct: 541  HQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFLAGVL 600

Query: 601  AWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVASVEKD 660
            AWKDPHILIP+LQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTA+Q ASVEKD
Sbjct: 601  AWKDPHILIPALQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTASQAASVEKD 660

Query: 661  NNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSINSNLRTSVSA 720
            NNSASG SSRTRRYRRRSGNM S+GSSLDENKNPV   SVPQGSVEVPSINSNLRT+VSA
Sbjct: 661  NNSASGTSSRTRRYRRRSGNMNSDGSSLDENKNPV---SVPQGSVEVPSINSNLRTAVSA 720

Query: 721  CANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGSCIDDI 780
            CANAFKTKYFPLD GDVEVGVTDDLLRLKNLC KLNAGID QK+KSKGK KASGS +DDI
Sbjct: 721  CANAFKTKYFPLDQGDVEVGVTDDLLRLKNLCVKLNAGIDDQKTKSKGKLKASGSRLDDI 780

Query: 781  ITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILEAELPK 840
            ITNK++YLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLL+YFSCGYFSKGRI EAELPK
Sbjct: 781  ITNKEEYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLNYFSCGYFSKGRISEAELPK 840

Query: 841  IRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSRSSSGS 900
            +RQQVLKRFKS VSVAL TSINEGTVA M VLVQKLQSALSSLERFPVVLSHSSRSS+GS
Sbjct: 841  LRQQVLKRFKSFVSVALPTSINEGTVAHMRVLVQKLQSALSSLERFPVVLSHSSRSSNGS 900

Query: 901  ARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSE-G 960
            ARLSSGLSVLSQPFKLRLCRA G+K LRDYSSNIVLIDPLASLAAVEEFLWPRVQ+SE G
Sbjct: 901  ARLSSGLSVLSQPFKLRLCRAQGDKSLRDYSSNIVLIDPLASLAAVEEFLWPRVQKSESG 960

Query: 961  QKLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELSQDKST 1020
            QK S SGANSDSGTTPPGTVAPSGL STPSSTT RYSTRSRSSMTIGER+GKE SQ+KST
Sbjct: 961  QKPSASGANSDSGTTPPGTVAPSGLNSTPSSTTRRYSTRSRSSMTIGERSGKESSQEKST 1020

Query: 1021 SKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDD 1080
            SKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDD
Sbjct: 1021 SKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDLSSIQIDD 1080

Query: 1081 SLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATSDSQIHST 1140
            SLVIEDDDISDDEDDDHDD   DDSLPLCMPEKVHDVKL D  EDGDAGPATSDSQIHST
Sbjct: 1081 SLVIEDDDISDDEDDDHDD---DDSLPLCMPEKVHDVKLSDGVEDGDAGPATSDSQIHST 1140

Query: 1141 FGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRDPQGHPLF 1200
            FGSSSR AT+RGSNSPDHR GNS+S+RG M+FAAAAMAGLGPANG+ FRGGRDPQG PLF
Sbjct: 1141 FGSSSRAATVRGSNSPDHRSGNSYSSRGGMSFAAAAMAGLGPANGKAFRGGRDPQGRPLF 1200

Query: 1201 GGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSNDGSSLWGD 1260
            G SNDNPKL+FSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAG+DFLSNDGSSLWGD
Sbjct: 1201 GSSNDNPKLVFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGSDFLSNDGSSLWGD 1260

Query: 1261 IYTITYQRADNQSERAALAGESSSSKSKSTKCVST--SNSNFRFHQMSLLDSILQGKLPC 1320
            IYTITYQRADNQSER ALAGESSSSKSKSTKCVST  SNS  +FHQMSLLDSILQGKLPC
Sbjct: 1261 IYTITYQRADNQSER-ALAGESSSSKSKSTKCVSTSGSNSESQFHQMSLLDSILQGKLPC 1320

Query: 1321 DLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEF 1380
            DLDK+NPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEF
Sbjct: 1321 DLDKTNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGKVPHEEF 1380

Query: 1381 VNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALY 1440
            +NSKLTPKL RQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALY
Sbjct: 1381 INSKLTPKLARQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFGLSRALY 1440

Query: 1441 RLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVLEVEYFG 1500
            RLHQQ GADGLGTVNEREGRVGRLQRQKVRVSRNRILDSA KVMEMYS+QKAVLEVEYFG
Sbjct: 1441 RLHQQQGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFG 1500

Query: 1501 EVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGN 1560
            EVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESI+S EDGQLRK K GS+LISDAGN
Sbjct: 1501 EVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIESDEDGQLRKAKGGSRLISDAGN 1560

Query: 1561 IDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFY 1620
            IDIIQSPLGLFPRPW P+ADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFY
Sbjct: 1561 IDIIQSPLGLFPRPWSPDADSSDGSQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFY 1620

Query: 1621 KLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVED 1680
            KLVLGQDLDL+DILSFD ELGKTLQELQALVCRKQYL L++GDNQNT+SNLSFRGIPVED
Sbjct: 1621 KLVLGQDLDLHDILSFDAELGKTLQELQALVCRKQYLVLINGDNQNTVSNLSFRGIPVED 1680

Query: 1681 LCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDI 1740
            LCLDFT+PGYP+YVL+PGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFT GFNQVFDI
Sbjct: 1681 LCLDFTLPGYPNYVLKPGDETVNIHNLEEYISLVLDATVKTGIMRQMEAFTAGFNQVFDI 1740

Query: 1741 TALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQR 1800
            TALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGE TPEQQR
Sbjct: 1741 TALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLEIMGEFTPEQQR 1800

Query: 1801 GFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCAN 1860
             FCQFVTGAPRLPPGGLAVLNPRLTIVRKHSS+A+NAA S TG SESADDDLPSVMTCAN
Sbjct: 1801 AFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSSASNAATSGTGASESADDDLPSVMTCAN 1860

Query: 1861 YLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            YLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1861 YLKLPPYSTKEIMYKKLIYAINEGQGSFDLS 1883

BLAST of Carg16175 vs. TAIR 10
Match: AT4G38600.1 (HEAT repeat ;HECT-domain (ubiquitin-transferase) )

HSP 1 Score: 2421.4 bits (6274), Expect = 0.0e+00
Identity = 1343/1917 (70.06%), Postives = 1564/1917 (81.59%), Query Frame = 0

Query: 1    METRSRKRAEASSAAPSSSS---------SGPTTRSSKRARLSGTFSSNLAASTLSISTR 60
            METRSRKRAEA+SAAPSSSS         SGPTTR SKRARLS + SS+LA +  S ST 
Sbjct: 1    METRSRKRAEATSAAPSSSSSSPPPPPSASGPTTR-SKRARLSSSSSSSLAPTPPSSSTT 60

Query: 61   SRSTRNQEPSATSTPMDSTNESSGSRRRG---KNSDKENSDKGKEKEHEVRIGDRERNAE 120
            +RS R+   +A + PMD++ +SSG RR G   + ++ +NSDKGKEKEH+VRI +RER  +
Sbjct: 61   TRS-RSSRSAAAAAPMDTSTDSSGFRRGGRGNRGNNNDNSDKGKEKEHDVRIRERERERD 120

Query: 121  QS-FGLNIEGSGGG-----DDDDNDSE-GGMGVLQQNLSTASSALQGLLRKLGAGLDDLL 180
            ++   LN++ +        +DDDNDSE G  G +  N+S+ASSALQGLLRKLGAGLDDLL
Sbjct: 121  RAREQLNMDAAAAAARSADEDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLL 180

Query: 181  PSSAIASASSSQQRGRLKKILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSF 240
            PSS I SASSS   GR+KKILSGLRA+GEEG+QVEALTQLCEMLSIGTE+SLSTFSVDSF
Sbjct: 181  PSSGIGSASSSHLNGRMKKILSGLRAEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSF 240

Query: 241  VPVLVGLLNHESNPDIMLLAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLA 300
            VPVLVGLLNHESNPDIMLLAARALTHLCD LPSSCAAVVHYGAVSC  ARLLTIEYMDLA
Sbjct: 241  VPVLVGLLNHESNPDIMLLAARALTHLCDVLPSSCAAVVHYGAVSCLVARLLTIEYMDLA 300

Query: 301  EQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADF 360
            EQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDA+D+
Sbjct: 301  EQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDY 360

Query: 361  VMEAVPLLTNLLQYHDAKVLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTS 420
            VMEAVPLLTNLLQYHD+KVLE+AS+CLTRIAEAFA  P+KLDELCNHGLVTQAASLISTS
Sbjct: 361  VMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTS 420

Query: 421  STGGGQSTLSSATYTGLIRLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVS 480
            ++GGGQ++LS +TYTGLIRLLSTCASGS LG +TLLLLGIS ILKDIL GSGVS N+SVS
Sbjct: 421  NSGGGQASLSVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDILLGSGVSANASVS 480

Query: 481  PALNRPQEQIFEIVNLANELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTD 540
            PAL+RP +QI+EIVNLANELLPPLP+G IS P + N  VKG   KK S S S K+ED   
Sbjct: 481  PALSRPADQIYEIVNLANELLPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQED--- 540

Query: 541  SAPEMSAREKLLKDQPELLHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPE 600
               ++S REKLL DQPELL QFG+DLLP+L+QIYGSSVNG +RHKCLS IGKLMYFS+ E
Sbjct: 541  -ILKISPREKLLGDQPELLQQFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSE 600

Query: 601  MIQSLLNVTNIASFLAGVLAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQ 660
            MIQSL+  TNI+SFLAGVLAWKDP +L+P+LQ+AEILMEKLPETFSK+F+REGVV+AVDQ
Sbjct: 601  MIQSLIGDTNISSFLAGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQ 660

Query: 661  LILANNQNTATQVASVEKDNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVS-GGSV 720
            L+L    + A   +  +KDN+   G S+R+RRYRRRS N  S+G+  +E KNP S     
Sbjct: 661  LVLVGKPSHA---SPTDKDNDCVPG-SARSRRYRRRSSNANSDGNQSEEPKNPASLTIGA 720

Query: 721  PQGSVEVPSINSNLRTSVSACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGID 780
               S++ P+ +  LR +VS+CA AFK KYFP D GDV+VGVTDDLL LKNLC KL AGID
Sbjct: 721  NHNSLDTPTASFMLRETVSSCAKAFKDKYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGID 780

Query: 781  VQKSKSKGKSKASGSCIDDIITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLL 840
              K K KGKSKASG  + D   +K++YL GVISE+L E+ K DGVSTFEFIGSGVV  LL
Sbjct: 781  DHKVKGKGKSKASGPFLGDFSASKEEYLIGVISEILGEISKGDGVSTFEFIGSGVVAALL 840

Query: 841  DYFSCGYFSKGRILEAELPKIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSAL 900
            +YFSCGYFSK +I E  LPK+RQ+ L+RFK+ + VAL    NEG V PMTVL+QKLQ+AL
Sbjct: 841  NYFSCGYFSKEKISELNLPKLRQEGLRRFKAFLEVALPFDGNEGKVPPMTVLIQKLQNAL 900

Query: 901  SSLERFPVVLSHSSRSSSGSARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPL 960
            SSLERFPVVLSH SRS SGSARLSSGLS L+ P KLRLCRA GEK LRDYSSNIVLIDPL
Sbjct: 901  SSLERFPVVLSHPSRSLSGSARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPL 960

Query: 961  ASLAAVEEFLWPRVQRSEGQ-KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRS 1020
            ASLAAVEEFLWPRVQRSE   K +    N++ GT P G    S   STP+STT R+S+RS
Sbjct: 961  ASLAAVEEFLWPRVQRSESALKPAAPIGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRS 1020

Query: 1021 RSSMTIGERAGKELSQDK--STSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMK 1080
            RS++ IG+ + K+   +K  S+SKGKGK ++KPA  +K G QTRS+ ++RA +DKD QMK
Sbjct: 1021 RSAINIGDTSKKDPVHEKGTSSSKGKGKGVMKPAQADK-GPQTRSNAQKRAVLDKDTQMK 1080

Query: 1081 PVNGETTSEDEELDLSSIQIDDSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVK 1140
            P +G+++SEDEEL++S + IDD+LVIE+DDISDDEDDD++DVL DDSLP+C P+KVHDVK
Sbjct: 1081 PASGDSSSEDEELEISPVDIDDALVIEEDDISDDEDDDNEDVL-DDSLPMCTPDKVHDVK 1140

Query: 1141 LGDTAEDGDAGPATSDSQIHSTFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMA 1200
            L D+ +D   G ATS  Q++   G +S  A  R S+S D   GNS+ +RG+++FAAAAMA
Sbjct: 1141 LADSVDDD--GLATSGRQMNPASGGTSGAAAARASDSIDTGIGNSYGSRGALSFAAAAMA 1200

Query: 1201 GLGPANGRGFRGGRDPQGHPLFGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDD 1260
            GLG A+GRG RG RD  G  L   S++  KL+F++  KQL RHLTIYQAVQRQL+L+EDD
Sbjct: 1201 GLGAASGRGIRGSRDLHGRTLNRSSDEPSKLIFTAAGKQLSRHLTIYQAVQRQLMLDEDD 1260

Query: 1261 DERFAGAGNDFLSNDGSSLWGDIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNS 1320
            D+RF   G+D +S+DGS  + DIYTI YQR D+Q  R ++ G SS++ SKSTK  +T++S
Sbjct: 1261 DDRF--GGSDLVSSDGSR-FNDIYTIMYQRPDSQVNRLSVGGASSTTPSKSTKSATTNSS 1320

Query: 1321 -NFRFHQMSLLDSILQGKLPCDLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAE 1380
               + H+ SLLDSILQG+LPCDL+KSN TY++L+LLRVLEGLNQL PRLRAQ +SD+FAE
Sbjct: 1321 VESQSHRASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLCPRLRAQTLSDRFAE 1380

Query: 1381 GKITTLDELGGIGGKVPHEEFVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLF 1440
            GKIT+LD+L     KVP +EFVNSKLTPKL RQIQDALALCSGSLPSWCYQLT+ACPFLF
Sbjct: 1381 GKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQIQDALALCSGSLPSWCYQLTRACPFLF 1440

Query: 1441 PFETRRQYFYSTAFGLSRALYRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDS 1500
            PF+TRRQYFYSTAFGLSRAL RL QQ GADG G+ NERE R+GRLQRQKVRVSRNRILDS
Sbjct: 1441 PFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDS 1500

Query: 1501 AVKVMEMYSNQKAVLEVEYFGEVGTGLGPTLEFYTLLSHELQRAGLGMWRSN-----SLQ 1560
            A KVMEMYS+QKAVLEVEYFGEVGTGLGPTLEFYTLLSH+LQ+A LGMWRS+     S+Q
Sbjct: 1501 AAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKASLGMWRSSSGDKVSMQ 1560

Query: 1561 ESIDSGEDGQLRKPKDGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYF 1620
               D  EDG   KP         A N DI+ +PLGLFPRPWP  AD S+G QF KVIEYF
Sbjct: 1561 IGRDEIEDG---KP--------SAANRDIVLAPLGLFPRPWPSTADISEGGQFHKVIEYF 1620

Query: 1621 RLVGRVMAKALQDGRLLDLPLSTAFYKLVLGQDLDLYDILSFDPELGKTLQELQALVCRK 1680
            RL+GRVMAKALQDGRLLD+PLSTAFYKL+LGQ+LDL+DI+ FD ELGKTLQEL+ +V RK
Sbjct: 1621 RLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTLQELRVVVARK 1680

Query: 1681 QYLGLVDGDNQNTISNLSFRGIPVEDLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLV 1740
             YL  V GDN +TIS+L  RG  +EDL L+FT+PGYP+Y+LR GDE V+I NLEEYISLV
Sbjct: 1681 HYLEGVGGDNSSTISDLCLRGCRIEDLSLEFTLPGYPEYILRSGDEIVDITNLEEYISLV 1740

Query: 1741 LDATVKTGIMRQMEAFTTGFNQVFDITALRIFIPHELDHLLCGRRELWKADTLVDHIKFD 1800
            +DATVK G+ RQ+EAF +GFNQVFDIT+L+IF P ELD+LLCGRRELW+ +TL +HIKFD
Sbjct: 1741 VDATVKRGVTRQIEAFRSGFNQVFDITSLQIFTPSELDYLLCGRRELWEVETLAEHIKFD 1800

Query: 1801 HGYTAKSPAIVNFLEIMGELTPEQQRGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTA 1860
            HGY AKSPAI+N LEIMGELT +QQR FCQFVTGAPRLPPGGLAVLNP+LTIVRKHSST+
Sbjct: 1801 HGYNAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTS 1860

Query: 1861 TNAANSATGVSESADDDLPSVMTCANYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            + AAN A G SE+ADDDLPSVMTCANYLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1861 SAAANGA-GASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888

BLAST of Carg16175 vs. TAIR 10
Match: AT4G38600.2 (HEAT repeat ;HECT-domain (ubiquitin-transferase) )

HSP 1 Score: 2336.2 bits (6053), Expect = 0.0e+00
Identity = 1287/1842 (69.87%), Postives = 1495/1842 (81.16%), Query Frame = 0

Query: 67   MDSTNESSGSRRRG---KNSDKENSDKGKEKEHEVRIGDRERNAEQS-FGLNIEGSGGG- 126
            MD++ +SSG RR G   + ++ +NSDKGKEKEH+VRI +RER  +++   LN++ +    
Sbjct: 1    MDTSTDSSGFRRGGRGNRGNNNDNSDKGKEKEHDVRIRERERERDRAREQLNMDAAAAAA 60

Query: 127  ----DDDDNDSE-GGMGVLQQNLSTASSALQGLLRKLGAGLDDLLPSSAIASASSSQQRG 186
                +DDDNDSE G  G +  N+S+ASSALQGLLRKLGAGLDDLLPSS I SASSS   G
Sbjct: 61   RSADEDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNG 120

Query: 187  RLKKILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLNHESNPD 246
            R+KKILSGLRA+GEEG+QVEALTQLCEMLSIGTE+SLSTFSVDSFVPVLVGLLNHESNPD
Sbjct: 121  RMKKILSGLRAEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPD 180

Query: 247  IMLLAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKKISQEHP 306
            IMLLAARALTHLCD LPSSCAAVVHYGAVSC  ARLLTIEYMDLAEQSLQALKKISQEHP
Sbjct: 181  IMLLAARALTHLCDVLPSSCAAVVHYGAVSCLVARLLTIEYMDLAEQSLQALKKISQEHP 240

Query: 307  TACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLTNLLQYH 366
            TACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDA+D+VMEAVPLLTNLLQYH
Sbjct: 241  TACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYH 300

Query: 367  DAKVLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTLSSATYT 426
            D+KVLE+AS+CLTRIAEAFA  P+KLDELCNHGLVTQAASLISTS++GGGQ++LS +TYT
Sbjct: 301  DSKVLEYASICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVSTYT 360

Query: 427  GLIRLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQIFEIVN 486
            GLIRLLSTCASGS LG +TLLLLGIS ILKDIL GSGVS N+SVSPAL+RP +QI+EIVN
Sbjct: 361  GLIRLLSTCASGSPLGFRTLLLLGISSILKDILLGSGVSANASVSPALSRPADQIYEIVN 420

Query: 487  LANELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSAREKLLKDQ 546
            LANELLPPLP+G IS P + N  VKG   KK S S S K+ED      ++S REKLL DQ
Sbjct: 421  LANELLPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQED----ILKISPREKLLGDQ 480

Query: 547  PELLHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVTNIASFL 606
            PELL QFG+DLLP+L+QIYGSSVNG +RHKCLS IGKLMYFS+ EMIQSL+  TNI+SFL
Sbjct: 481  PELLQQFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSEMIQSLIGDTNISSFL 540

Query: 607  AGVLAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNTATQVAS 666
            AGVLAWKDP +L+P+LQ+AEILMEKLPETFSK+F+REGVV+AVDQL+L    + A   + 
Sbjct: 541  AGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPSHA---SP 600

Query: 667  VEKDNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVS-GGSVPQGSVEVPSINSNLR 726
             +KDN+   G S+R+RRYRRRS N  S+G+  +E KNP S        S++ P+ +  LR
Sbjct: 601  TDKDNDCVPG-SARSRRYRRRSSNANSDGNQSEEPKNPASLTIGANHNSLDTPTASFMLR 660

Query: 727  TSVSACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKSKASGS 786
             +VS+CA AFK KYFP D GDV+VGVTDDLL LKNLC KL AGID  K K KGKSKASG 
Sbjct: 661  ETVSSCAKAFKDKYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGIDDHKVKGKGKSKASGP 720

Query: 787  CIDDIITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYFSKGRILE 846
             + D   +K++YL GVISE+L E+ K DGVSTFEFIGSGVV  LL+YFSCGYFSK +I E
Sbjct: 721  FLGDFSASKEEYLIGVISEILGEISKGDGVSTFEFIGSGVVAALLNYFSCGYFSKEKISE 780

Query: 847  AELPKIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERFPVVLSHSSR 906
              LPK+RQ+ L+RFK+ + VAL    NEG V PMTVL+QKLQ+ALSSLERFPVVLSH SR
Sbjct: 781  LNLPKLRQEGLRRFKAFLEVALPFDGNEGKVPPMTVLIQKLQNALSSLERFPVVLSHPSR 840

Query: 907  SSSGSARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAVEEFLWPRVQ 966
            S SGSARLSSGLS L+ P KLRLCRA GEK LRDYSSNIVLIDPLASLAAVEEFLWPRVQ
Sbjct: 841  SLSGSARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQ 900

Query: 967  RSEGQ-KLSVSGANSDSGTTPPGTVAPSGLKSTPSSTTCRYSTRSRSSMTIGERAGKELS 1026
            RSE   K +    N++ GT P G    S   STP+STT R+S+RSRS++ IG+ + K+  
Sbjct: 901  RSESALKPAAPIGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRSRSAINIGDTSKKDPV 960

Query: 1027 QDK--STSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTSEDEELDL 1086
             +K  S+SKGKGK ++KPA  +K G QTRS+ ++RA                  DEEL++
Sbjct: 961  HEKGTSSSKGKGKGVMKPAQADK-GPQTRSNAQKRA------------------DEELEI 1020

Query: 1087 SSIQIDDSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDGDAGPATS 1146
            S + IDD+LVIE+DDISDDEDDD++DVL DDSLP+C P+KVHDVKL D+ +D   G ATS
Sbjct: 1021 SPVDIDDALVIEEDDISDDEDDDNEDVL-DDSLPMCTPDKVHDVKLADSVDDD--GLATS 1080

Query: 1147 DSQIHSTFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGRGFRGGRD 1206
              Q++   G +S  A  R S+S D   GNS+ +RG+++FAAAAMAGLG A+GRG RG RD
Sbjct: 1081 GRQMNPASGGTSGAAAARASDSIDTGIGNSYGSRGALSFAAAAMAGLGAASGRGIRGSRD 1140

Query: 1207 PQGHPLFGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAGNDFLSND 1266
              G  L   S++  KL+F++  KQL RHLTIYQAVQRQL+L+EDDD+RF   G+D +S+D
Sbjct: 1141 LHGRTLNRSSDEPSKLIFTAAGKQLSRHLTIYQAVQRQLMLDEDDDDRF--GGSDLVSSD 1200

Query: 1267 GSSLWGDIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNS-NFRFHQMSLLDSIL 1326
            GS  + DIYTI YQR D+Q  R ++ G SS++ SKSTK  +T++S   + H+ SLLDSIL
Sbjct: 1201 GSR-FNDIYTIMYQRPDSQVNRLSVGGASSTTPSKSTKSATTNSSVESQSHRASLLDSIL 1260

Query: 1327 QGKLPCDLDKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDELGGIGGK 1386
            QG+LPCDL+KSN TY++L+LLRVLEGLNQL PRLRAQ +SD+FAEGKIT+LD+L     K
Sbjct: 1261 QGELPCDLEKSNSTYNVLALLRVLEGLNQLCPRLRAQTLSDRFAEGKITSLDDLSTTAAK 1320

Query: 1387 VPHEEFVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQYFYSTAFG 1446
            VP +EFVNSKLTPKL RQIQDALALCSGSLPSWCYQLT+ACPFLFPF+TRRQYFYSTAFG
Sbjct: 1321 VPLDEFVNSKLTPKLARQIQDALALCSGSLPSWCYQLTRACPFLFPFQTRRQYFYSTAFG 1380

Query: 1447 LSRALYRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMYSNQKAVL 1506
            LSRAL RL QQ GADG G+ NERE R+GRLQRQKVRVSRNRILDSA KVMEMYS+QKAVL
Sbjct: 1381 LSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVL 1440

Query: 1507 EVEYFGEVGTGLGPTLEFYTLLSHELQRAGLGMWRSN-----SLQESIDSGEDGQLRKPK 1566
            EVEYFGEVGTGLGPTLEFYTLLSH+LQ+A LGMWRS+     S+Q   D  EDG   KP 
Sbjct: 1441 EVEYFGEVGTGLGPTLEFYTLLSHDLQKASLGMWRSSSGDKVSMQIGRDEIEDG---KP- 1500

Query: 1567 DGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQDGR 1626
                    A N DI+ +PLGLFPRPWP  AD S+G QF KVIEYFRL+GRVMAKALQDGR
Sbjct: 1501 -------SAANRDIVLAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGR 1560

Query: 1627 LLDLPLSTAFYKLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNTIS 1686
            LLD+PLSTAFYKL+LGQ+LDL+DI+ FD ELGKTLQEL+ +V RK YL  V GDN +TIS
Sbjct: 1561 LLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTLQELRVVVARKHYLEGVGGDNSSTIS 1620

Query: 1687 NLSFRGIPVEDLCLDFTVPGYPDYVLRPGDETVNIHNLEEYISLVLDATVKTGIMRQMEA 1746
            +L  RG  +EDL L+FT+PGYP+Y+LR GDE V+I NLEEYISLV+DATVK G+ RQ+EA
Sbjct: 1621 DLCLRGCRIEDLSLEFTLPGYPEYILRSGDEIVDITNLEEYISLVVDATVKRGVTRQIEA 1680

Query: 1747 FTTGFNQVFDITALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIVNFLE 1806
            F +GFNQVFDIT+L+IF P ELD+LLCGRRELW+ +TL +HIKFDHGY AKSPAI+N   
Sbjct: 1681 FRSGFNQVFDITSLQIFTPSELDYLLCGRRELWEVETLAEHIKFDHGYNAKSPAIIN--- 1740

Query: 1807 IMGELTPEQQRGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVSESAD 1866
            IMGELT +QQR FCQFVTGAPRLPPGGLAVLNP+LTIVRKHSST++ AAN A G SE+AD
Sbjct: 1741 IMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGA-GASETAD 1794

Query: 1867 DDLPSVMTCANYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            DDLPSVMTCANYLKLPPYSTKEIMYKKL+YAINEGQGSFDLS
Sbjct: 1801 DDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1794

BLAST of Carg16175 vs. TAIR 10
Match: AT5G02880.1 (ubiquitin-protein ligase 4 )

HSP 1 Score: 878.2 bits (2268), Expect = 1.1e-254
Identity = 627/1726 (36.33%), Postives = 877/1726 (50.81%), Query Frame = 0

Query: 171  SSQQRGRLKKILSGLRADGEEGRQVEALTQLCEMLSIGTEESLSTFSVDSFVPVLVGLLN 230
            SS   G+LK +L  L  + +   Q+  LT+LCE+LS  TEESLS+   +   PVLV L  
Sbjct: 104  SSGDHGKLKSLLLNLTGETDPSGQLSRLTELCEVLSFSTEESLSSVMANMLSPVLVKLAK 163

Query: 231  HESNPDIMLLAARALTHLCDFLPSSCAAVVHYGAVSCFCARLLTIEYMDLAEQSLQALKK 290
            HE+N DIMLLA RA+T+LCD  P S   +V +  +   C RLLTIEY+D+AEQ LQAL+K
Sbjct: 164  HENNADIMLLAIRAITYLCDVYPPSVEFLVRHDTIPALCQRLLTIEYLDVAEQCLQALEK 223

Query: 291  ISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDAADFVMEAVPLLT 350
            IS++ P ACL AGA+MAVLS++DFFST +QRVA+ST  N+CK+L S++    M+AVP+L 
Sbjct: 224  ISRDEPVACLNAGAIMAVLSFIDFFSTSIQRVAISTVVNICKQLSSESPSPFMDAVPILC 283

Query: 351  NLLQYHDAKVLEHASVCLTRIAEAFASSPDKLDELCNHGLVTQAASLISTSSTGGGQSTL 410
             LLQY D +++E+ ++CLT+IA+  + SP  LD+LC HGL+ ++  L++ +S    ++TL
Sbjct: 284  TLLQYEDRQLVENVAICLTKIADQASESPAMLDQLCRHGLINESTHLLNLNS----RTTL 343

Query: 411  SSATYTGLIRLLSTCASGSALGGKTLLLLGISGILKDILSGSGVSTNSSVSPALNRPQEQ 470
            S   Y G+I +L   +SGSAL  +TL  L I   LK+I+S   +S + S +  +N    Q
Sbjct: 344  SQPVYNGVIGMLRKLSSGSALAFRTLYELNIGYSLKEIMSTYDISHSVSSTHPINACSNQ 403

Query: 471  IFEIVNLANELLPPLPQGTISFPPNYNLFVKGPVIKKPSTSGSVKEEDPTDSAPEMSARE 530
            + E++ L  ELLP                                   P +     S +E
Sbjct: 404  VHEVLKLVIELLP---------------------------------ASPVEDNQLASEKE 463

Query: 531  KLLKDQPELLHQFGMDLLPILIQIYGSSVNGPVRHKCLSGIGKLMYFSTPEMIQSLLNVT 590
              L +QP+LL QFG D+LP++IQ+  S  N  V + CLS I KL   S    I  LL  T
Sbjct: 464  SFLVNQPDLLQQFGRDMLPVMIQVLNSGANVYVSYGCLSAIHKLTCLSKSGDIVELLKNT 523

Query: 591  NIASFLAGVLAWKDPHILIPSLQIAEILMEKLPETFSKMFLREGVVYAVDQLILANNQNT 650
            N++S LAG+L+ KD H+++ +LQ+AE+L+EK  +TF   F++EGV +A++ L+ ++    
Sbjct: 524  NMSSVLAGILSRKDHHVIVVALQVAEVLLEKYRDTFLNSFIKEGVFFAIEALLSSDRGQQ 583

Query: 651  ATQVASVEKDNNSASGASSRTRRYRRRSGNMTSEGSSLDENKNPVSGGSVPQGSVEVPSI 710
                A + +   +        + + R   +++S   +    K+                 
Sbjct: 584  NQGSADLSQKPVTKEIVKCLCQSFER---SLSSSSQTCKIEKD----------------- 643

Query: 711  NSNLRTSVSACANAFKTKYFPLDPGDVEVGVTDDLLRLKNLCAKLNAGIDVQKSKSKGKS 770
                  SV   A   K  +F  +  + E G+TD L  LKNL   L+  + V         
Sbjct: 644  ------SVYVLATRIKEGFFGPEVFNSEKGLTDVLQNLKNLSVALSELMTVP-------- 703

Query: 771  KASGSCIDDIITNKDQYLTGVISEMLVELGKDDGVSTFEFIGSGVVGVLLDYFSCGYF-- 830
                  ID  + + +++ + + ++++  L   + VSTFEFI SGVV  L  Y S G +  
Sbjct: 704  ------IDAHVLHDEKFFS-IWNQIMERLNGRESVSTFEFIESGVVKSLASYLSNGLYQR 763

Query: 831  --SKGRILEAELPKIRQQVLKRFKSLVSVALSTSINEGTVAPMTVLVQKLQSALSSLERF 890
              SKG      LP I     KRF+  V   L  S  E T    ++L+QKLQ++LSSLE F
Sbjct: 764  KLSKGGPECDSLPFIG----KRFE--VFTRLLWSDGEAT---SSLLIQKLQNSLSSLENF 823

Query: 891  PVVLSHSSRSSSGSARLSSGLSVLSQPFKLRLCRAHGEKLLRDYSSNIVLIDPLASLAAV 950
            P+VLS   +  +  A + +G        K+R  +A GE  LRDYS + V +DPL  L AV
Sbjct: 824  PIVLSQFLKQKNSFAAIPNGRCTSYPCLKVRFLKAEGETSLRDYSQDFVTVDPLCYLDAV 883

Query: 951  EEFLWPRVQRSEGQKLSVSGANSDSGTTPPGTVAP-SGLKSTPSSTTCRYSTRSRSSMTI 1010
            +++LWP+V                        + P   +++   +  C+ S    +S++ 
Sbjct: 884  DQYLWPKV-----------------------NIEPIDSVEAKDQAIECQSSQLQSTSISC 943

Query: 1011 GERAGKELSQDKSTSKGKGKAILKPAWEEKRGLQTRSSTRRRAAVDKDAQMKPVNGETTS 1070
               +   +  D  +S             + +G Q    T+         Q    + ET+S
Sbjct: 944  QAESSSPMEIDSESSDA----------SQLQGSQVEDQTQ------LPGQQNASSSETSS 1003

Query: 1071 EDEELDLSSIQIDDSLVIEDDDISDDEDDDHDDVLQDDSLPLCMPEKVHDVKLGDTAEDG 1130
            E E+                                                        
Sbjct: 1004 EKEDA------------------------------------------------------- 1063

Query: 1131 DAGPATSDSQIHSTFGSSSRTATIRGSNSPDHRGGNSFSARGSMTFAAAAMAGLGPANGR 1190
                                                                        
Sbjct: 1064 ------------------------------------------------------------ 1123

Query: 1191 GFRGGRDPQGHPLFGGSNDNPKLLFSSGEKQLDRHLTIYQAVQRQLVLNEDDDERFAGAG 1250
                                P+LLF     +LDR LT+YQA+    + +E +      A 
Sbjct: 1124 -------------------VPRLLFRLEGLELDRSLTVYQAILLHKLKSESE------AT 1183

Query: 1251 NDFLSNDGSSLWGDIYTITYQRADNQSERAALAGESSSSKSKSTKCVSTSNSNFRFHQMS 1310
            ND      S L G  + ITY       ER+A  G+S  +        S  +  +R     
Sbjct: 1184 ND------SKLSGP-HNITY-------ERSAQLGDSRENLFPPG---SMEDDEYR----P 1243

Query: 1311 LLDSILQGKLPCDL-DKSNPTYDILSLLRVLEGLNQLAPRLRAQIVSDQFAEGKITTLDE 1370
             L  +   +L   L   S+P YDIL LL+ LEG+N+    L +    + F EG++  LD+
Sbjct: 1244 FLSYLFTHRLALRLKGSSHPPYDILFLLKSLEGMNRFLFHLISLERINAFGEGRLENLDD 1303

Query: 1371 LGGIGGKVPHEEFVNSKLTPKLTRQIQDALALCSGSLPSWCYQLTKACPFLFPFETRRQY 1430
            L      VPH EFV+SKLT KL +Q++D+ A+ +  LP W   L  +CP LF FE + +Y
Sbjct: 1304 LRVQVRPVPHSEFVSSKLTEKLEQQLRDSFAVSTCGLPPWFNDLMDSCPCLFSFEAKSKY 1363

Query: 1431 FYSTAFGLSRALYRLHQQLGADGLGTVNEREGRVGRLQRQKVRVSRNRILDSAVKVMEMY 1490
            F   AFG S+ +    Q L +  +    E     G L R+K    R  IL+SA K+ME+Y
Sbjct: 1364 FRLAAFG-SQKIRHHPQHLSSSNVH--GEARPVTGSLPRKKFLACRENILESAAKMMELY 1423

Query: 1491 SNQKAVLEVEYFGEVGTGLGPTLEFYTLLSHELQRAGLGMWRSNSLQESIDSGEDGQLRK 1550
             NQK V+EVEY  EVGTGLGPTLEFYTL+S   Q   LGMWR++    S   G      K
Sbjct: 1424 GNQKVVIEVEYSEEVGTGLGPTLEFYTLVSRAFQNPDLGMWRNDC---SFIVG------K 1483

Query: 1551 PKDGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDGSQFSKVIEYFRLVGRVMAKALQD 1610
            P + S          ++ S  GLFPRPW   + +SD      V++ F L+G V+AKALQD
Sbjct: 1484 PVEHS---------GVLASSSGLFPRPWSGTSTTSD------VLQKFVLLGTVVAKALQD 1502

Query: 1611 GRLLDLPLSTAFYKLVLGQDLDLYDILSFDPELGKTLQELQALVCRKQYLGLVDGDNQNT 1670
            GR+LDLPLS AFYKL+LGQ+L  +DI   DPEL KTL ELQALV RK+      GD+   
Sbjct: 1544 GRVLDLPLSKAFYKLILGQELSSFDIHFVDPELCKTLVELQALVRRKKLFAEAHGDSGAA 1502

Query: 1671 ISNLSFRGIPVEDLCLDFTVPGYPDYVLRP--GDETVNIHNLEEYISLVLDATVKTGIMR 1730
              +LSF G  +EDLCL+F +PGY DY L P   ++ VN+ NLEEYI  +++ATV  GI +
Sbjct: 1604 KCDLSFHGTKIEDLCLEFALPGYTDYDLAPYSANDMVNLDNLEEYIKGIVNATVCNGIQK 1502

Query: 1731 QMEAFTTGFNQVFDITALRIFIPHELDHLLCGRRELWKADTLVDHIKFDHGYTAKSPAIV 1790
            Q+EAF +GFNQVF I  LRIF   EL+ +LCG  +L+  + ++DHIKFDHGYT+ SP + 
Sbjct: 1664 QVEAFRSGFNQVFSIEHLRIFNEEELETMLCGECDLFSMNEVLDHIKFDHGYTSSSPPVE 1502

Query: 1791 NFLEIMGELTPEQQRGFCQFVTGAPRLPPGGLAVLNPRLTIVRKHSSTATNAANSATGVS 1850
              L+I+ E   EQQR F QFVTG+PRLP GGLA L+P+LTIVRKH              S
Sbjct: 1724 YLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRKHG-------------S 1502

Query: 1851 ESADDDLPSVMTCANYLKLPPYSTKEIMYKKLMYAINEGQGSFDLS 1889
            +S+D DLPSVMTCANYLKLPPYS+KE M +KL+YAI EGQGSF LS
Sbjct: 1784 DSSDTDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502

BLAST of Carg16175 vs. TAIR 10
Match: AT1G55860.1 (ubiquitin-protein ligase 1 )

HSP 1 Score: 136.0 bits (341), Expect = 3.2e-31
Identity = 120/430 (27.91%), Postives = 187/430 (43.49%), Query Frame = 0

Query: 1465 KVRVSRNRILDSAVKVMEMYSNQ--KAVLEVEYFGEVGTGLGP-TLEFYTLLSHELQRAG 1524
            ++ V R  +L+ +   + M S Q  K  L V++ GE G   G  T E+Y LLS  +    
Sbjct: 3569 RISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIF--- 3628

Query: 1525 LGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDG 1584
                                      G+ L +  GN    Q           PN +S   
Sbjct: 3629 ------------------------DKGALLFTTVGNDATFQ-----------PNPNSVYQ 3688

Query: 1585 SQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYKLVLGQDLDLYDILSFDPELGKTL 1644
            ++    + YF+ VGR++AKAL DG+LLD+  + +FYK +LG  +  +DI + DP+  K L
Sbjct: 3689 TEH---LSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNL 3748

Query: 1645 QELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDLCLDFTVPGYPDYVLRPGDETVNI 1704
            + L               ++ + I +L+F     E+  + +      DY L+PG   + +
Sbjct: 3749 KWLLE-------------NDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRV 3808

Query: 1705 --HNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDITALRIFIPHELDHLLCGRRELW 1764
                  EY+ LV    +   I  Q+ AF  GFN++     + IF   EL+ L+ G  E+ 
Sbjct: 3809 TEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELIPRELVSIFNDKELELLISGLPEI- 3868

Query: 1765 KADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRGFCQFVTGAPRLPPGGLAVL-- 1824
              D L  + ++   YTA SP I  F E++   + E    F QFVTG  ++P  G   L  
Sbjct: 3869 DFDDLKANTEYT-SYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQG 3927

Query: 1825 --NPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANYLKLPPYSTKEIMYKKLM 1884
               P+   + K               +  A + LPS  TC N L LP Y +KE + ++L+
Sbjct: 3929 ISGPQRLQIHK---------------AYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLL 3927

Query: 1885 YAINEGQGSF 1886
             AI+E    F
Sbjct: 3989 LAIHEASEGF 3927

BLAST of Carg16175 vs. TAIR 10
Match: AT1G55860.2 (ubiquitin-protein ligase 1 )

HSP 1 Score: 136.0 bits (341), Expect = 3.2e-31
Identity = 120/430 (27.91%), Postives = 187/430 (43.49%), Query Frame = 0

Query: 1465 KVRVSRNRILDSAVKVMEMYSNQ--KAVLEVEYFGEVGTGLGP-TLEFYTLLSHELQRAG 1524
            ++ V R  +L+ +   + M S Q  K  L V++ GE G   G  T E+Y LLS  +    
Sbjct: 3320 RISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIF--- 3379

Query: 1525 LGMWRSNSLQESIDSGEDGQLRKPKDGSKLISDAGNIDIIQSPLGLFPRPWPPNADSSDG 1584
                                      G+ L +  GN    Q           PN +S   
Sbjct: 3380 ------------------------DKGALLFTTVGNDATFQ-----------PNPNSVYQ 3439

Query: 1585 SQFSKVIEYFRLVGRVMAKALQDGRLLDLPLSTAFYKLVLGQDLDLYDILSFDPELGKTL 1644
            ++    + YF+ VGR++AKAL DG+LLD+  + +FYK +LG  +  +DI + DP+  K L
Sbjct: 3440 TEH---LSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNL 3499

Query: 1645 QELQALVCRKQYLGLVDGDNQNTISNLSFRGIPVEDLCLDFTVPGYPDYVLRPGDETVNI 1704
            + L               ++ + I +L+F     E+  + +      DY L+PG   + +
Sbjct: 3500 KWLLE-------------NDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRV 3559

Query: 1705 --HNLEEYISLVLDATVKTGIMRQMEAFTTGFNQVFDITALRIFIPHELDHLLCGRRELW 1764
                  EY+ LV    +   I  Q+ AF  GFN++     + IF   EL+ L+ G  E+ 
Sbjct: 3560 TEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELIPRELVSIFNDKELELLISGLPEI- 3619

Query: 1765 KADTLVDHIKFDHGYTAKSPAIVNFLEIMGELTPEQQRGFCQFVTGAPRLPPGGLAVL-- 1824
              D L  + ++   YTA SP I  F E++   + E    F QFVTG  ++P  G   L  
Sbjct: 3620 DFDDLKANTEYT-SYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQG 3678

Query: 1825 --NPRLTIVRKHSSTATNAANSATGVSESADDDLPSVMTCANYLKLPPYSTKEIMYKKLM 1884
               P+   + K               +  A + LPS  TC N L LP Y +KE + ++L+
Sbjct: 3680 ISGPQRLQIHK---------------AYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLL 3678

Query: 1885 YAINEGQGSF 1886
             AI+E    F
Sbjct: 3740 LAIHEASEGF 3678