Carg16161 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16161
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein of unknown function (DUF630 and DUF632)
LocationCarg_Chr20: 3611140 .. 3613893 (-)
RNA-Seq ExpressionCarg16161
SyntenyCarg16161
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGTGTTGCCTCAAAGTTGGAGGAAGAAGAAGAAGTAGTCTCCATCTGTAGAGAAAGAAAACGCCACATAAAGTTAGCTGTTGAGAGAAGGTATGCACTTGCAGAGGCACATAGTAAATATTGTCAAGCTTTATATGCTGTTTCAGCAGCCATTAAGCTCTTTGTAGCTCGCCATTCTTCTCCTTCCCCTTTCCTCATCACATTCCCTCCTACATGTTCTTCTTCTTCTCCTCCCCCTTCAGAAAAAGTGATATCCAATCCAATGTTTCTTCAGCAAAGGCCTTCAGATTCCACCCATGAAGCCATACCCGCCTGCCCTTCATGTGCTTGTTCTTCTTCTTCAACCTCCACAGAGTCCTCCATGGAAGAAAGAGAAGAAGAACCAGTAGAGGAGGAGAGGATTGAACAGGTCCCTGCATATTTCTATATGCAAATGCCACCCCCAATGCCATCCCCTCAGAGGGAGTTTAGTTGGGATTTTTTCAATCCATTTGATACCATGAGGACCGATGTCGTCTCCGGGTATCACCAGAGCTCGGAGGAGGAAGAAGGCATGCCAGAGCTGGAGGAAGAGACAGTAGAGAAAAACCAGAGAGTTGAGGATATTGGAGAGAAGGAAGAAGAGAGTCAGTTGAAGAAGAAGGAGCTAAGAGTCATTGATACACCTGTGGAAGGAAGGGAACTTTTGGAAGCATTGCAGGATGTTGAGGATTATTTCATTAGGGCTTATGATTCTGGAATTGATGTTTCTAGGATGTTAGAGGCCAACAAAATTCAGCTTCAATCTGGTTTAGAAGAAATAAAAGGTGTGCCATTCATATAGCTTCATTCTTTAAGCTCTCTAGTTTGTTTCTTGCAAGCTATATTGTCATTGAAGTAACAAGCTCATCATACTGCAGAGAACTCGACAAAACTCATCCAAGCGATAACTTGGCACCGGTCTGTTTCGGGCAAGACTTCATCATGTAAGAGTCTAGTAGCATCCAGTTCGAAAGGTTCTTCGCCATGGACGGAGTTTAGTAATGAGCTGTTTGATGATTATGACATAATGGACTCAGGAAGCCATTCGTCGACTCTTGGACGGTTATATGCTTGGGAGAAGAAACTCTACGAAGAGGTCAAGGTGAAGTAGGATCCTCAAGCTGTTTCTGTTTCTTTTGTTGAAAGATCAGTATAATAAGGAAAAACATCATAGTTTGATAGGGCTAGTCTATCAAACACGTTATAAGATATCATTTGATAACAGAGCCTGATTAGGTTCTCGTGTCGGTTGCTGCCACTCATTATGTTCTTTAGGGATGTAAACAGAATAAAGCTTTTATCCTTCTTGACTAGTGTTGGTAAATTGTGACTTACAACTTTCTGATTCTTGCACCCAGGCTGGGGACAGCATGCACAAACTGTATGAGAAAAGGTGTTCACGACTAAGAAACCAGGACGTTAAAGGGAGCAATGGAATGACAACTGATAAAACTCGGGTTATGGTTAAAGATTTATATGCCAGGATCTTGGTCGCCATTCGAAGTGCTGAATCAATCTCGACAAGAATTGTGAATCTAAGAGATGACGAGCTACAACCTCAGATCATAGAGCTATTAAAAGGGTAAACAGTGGAAGATACCTGTATTTCTGAAGTAAATAATGTATCCATTGTCAGTTTGTCACTTGTTTTTCTTCCCAAATCGTTTTGCAGCTTAACAAGAACTTGGAAAATCATGTTGGAAGTTCATGAAACCCAGAAAAAGATTATCCTTGAAGTGAAGACTTATTCCTGCCATTCCTATCTGAAGTTCTGTAACGAATCTCACCGTCTTGCAACTCTTCAGCTTGGAGCTGAGCTTCAGAATTGGCGTTCGTGTTTTTCAAAATATGTTGAATCACAGAAGGCTTATGTTGAAGCTCTCCATGGATGGCTGACCAAGTTTGTAGTGCCTGAGGTTGAGTTCTGTTCTCAGGGTCGGACTTCCGCTGTGCCTTATGGGCTGCACGGACCTCCATTGCTTTCAATCTGCCGTGATTGGCTATCATCCATGGAAAAATTACCAGATAAACCAGTAGCCTTTGCATTGAAAAGCTTTACAAAGGATATGAAGGCTCTATCAGATAAACAGATGGAGGAGCTGCAGCAAAAGAGAAGAGTTGAAAGCTTGGCAAAGGAACTCGACCGACGGATACTGTCATTCCAGAAAACAGAGAACAAGTTCCTCGAGTTCAACTTTACCGAGACGAAATCCGAGTTGGAAGTGGAAAATCAGAATGAGTACTTGACAGAAAAGAAAGATCAGCTGGACATGTTCAGGAAGAAGCTTGATTTGGAGAAGGAAAAACACAATAACTGCATTCAAGAAGCACAGAGGATTACATTAAATGGAATACAAACAGGGTTTTCCTCAGTTTTTGATTCCTTATCAGAGTTCTCCAAGGCTTCACAAAAAATGTACGACCACCTAGTGAATTACAGTGAGAATGCCAATAAATCTAAGAACATTAACTATATCGAGGGTTCCTCCCAAACTGAAGAAAATGTAAGCAGATAATGAAAGAAAAACCAGAAAATGGTTGGTTTTTCTTGGAGTTCTGTAAATTATATCATGTCGAAGCAGCTCGCTAAGACATTGGTTTGTAGTTTTATTCCAGCATCTCCTCCTAGAAAATTGTTAAGATTTCTGGTAGAGGCTTTTCATGCTCACAATTTTGTAGCAAAGGTCAATTTATTGCCATTTCTACATTATTGGCAAGAGGCCCAAGA

mRNA sequence

ATGGGTTGTGTTGCCTCAAAGTTGGAGGAAGAAGAAGAAGTAGTCTCCATCTGTAGAGAAAGAAAACGCCACATAAAGTTAGCTGTTGAGAGAAGGTATGCACTTGCAGAGGCACATAGTAAATATTGTCAAGCTTTATATGCTGTTTCAGCAGCCATTAAGCTCTTTGTAGCTCGCCATTCTTCTCCTTCCCCTTTCCTCATCACATTCCCTCCTACATGTTCTTCTTCTTCTCCTCCCCCTTCAGAAAAAGTGATATCCAATCCAATGTTTCTTCAGCAAAGGCCTTCAGATTCCACCCATGAAGCCATACCCGCCTGCCCTTCATGTGCTTGTTCTTCTTCTTCAACCTCCACAGAGTCCTCCATGGAAGAAAGAGAAGAAGAACCAGTAGAGGAGGAGAGGATTGAACAGGTCCCTGCATATTTCTATATGCAAATGCCACCCCCAATGCCATCCCCTCAGAGGGAGTTTAGTTGGGATTTTTTCAATCCATTTGATACCATGAGGACCGATGTCGTCTCCGGGTATCACCAGAGCTCGGAGGAGGAAGAAGGCATGCCAGAGCTGGAGGAAGAGACAGTAGAGAAAAACCAGAGAGTTGAGGATATTGGAGAGAAGGAAGAAGAGAGTCAGTTGAAGAAGAAGGAGCTAAGAGTCATTGATACACCTGTGGAAGGAAGGGAACTTTTGGAAGCATTGCAGGATGTTGAGGATTATTTCATTAGGGCTTATGATTCTGGAATTGATGTTTCTAGGATGTTAGAGGCCAACAAAATTCAGCTTCAATCTGGTTTAGAAGAAATAAAAGAGAACTCGACAAAACTCATCCAAGCGATAACTTGGCACCGGTCTGTTTCGGGCAAGACTTCATCATGTAAGAGTCTAGTAGCATCCAGTTCGAAAGGTTCTTCGCCATGGACGGAGTTTAGTAATGAGCTGTTTGATGATTATGACATAATGGACTCAGGAAGCCATTCGTCGACTCTTGGACGGTTATATGCTTGGGAGAAGAAACTCTACGAAGAGGTCAAGGCTGGGGACAGCATGCACAAACTGTATGAGAAAAGGTGTTCACGACTAAGAAACCAGGACGTTAAAGGGAGCAATGGAATGACAACTGATAAAACTCGGGTTATGGTTAAAGATTTATATGCCAGGATCTTGGTCGCCATTCGAAGTGCTGAATCAATCTCGACAAGAATTGTGAATCTAAGAGATGACGAGCTACAACCTCAGATCATAGAGCTATTAAAAGGCTTAACAAGAACTTGGAAAATCATGTTGGAAGTTCATGAAACCCAGAAAAAGATTATCCTTGAAGTGAAGACTTATTCCTGCCATTCCTATCTGAAGTTCTGTAACGAATCTCACCGTCTTGCAACTCTTCAGCTTGGAGCTGAGCTTCAGAATTGGCGTTCGTGTTTTTCAAAATATGTTGAATCACAGAAGGCTTATGTTGAAGCTCTCCATGGATGGCTGACCAAGTTTGTAGTGCCTGAGGTTGAGTTCTGTTCTCAGGGTCGGACTTCCGCTGTGCCTTATGGGCTGCACGGACCTCCATTGCTTTCAATCTGCCGTGATTGGCTATCATCCATGGAAAAATTACCAGATAAACCAGTAGCCTTTGCATTGAAAAGCTTTACAAAGGATATGAAGGCTCTATCAGATAAACAGATGGAGGAGCTGCAGCAAAAGAGAAGAGTTGAAAGCTTGGCAAAGGAACTCGACCGACGGATACTGTCATTCCAGAAAACAGAGAACAAGTTCCTCGAGTTCAACTTTACCGAGACGAAATCCGAGTTGGAAGTGGAAAATCAGAATGAGTACTTGACAGAAAAGAAAGATCAGCTGGACATGTTCAGGAAGAAGCTTGATTTGGAGAAGGAAAAACACAATAACTGCATTCAAGAAGCACAGAGGATTACATTAAATGGAATACAAACAGGGTTTTCCTCAGTTTTTGATTCCTTATCAGAGTTCTCCAAGGCTTCACAAAAAATGTACGACCACCTAGTGAATTACAGTGAGAATGCCAATAAATCTAAGAACATTAACTATATCGAGGGTTCCTCCCAAACTGAAGAAAATGTAAGCAGATAATGAAAGAAAAACCAGAAAATGGTTGGTTTTTCTTGGAGTTCTGTAAATTATATCATGTCGAAGCAGCTCGCTAAGACATTGGTTTGTAGTTTTATTCCAGCATCTCCTCCTAGAAAATTGTTAAGATTTCTGGTAGAGGCTTTTCATGCTCACAATTTTGTAGCAAAGGTCAATTTATTGCCATTTCTACATTATTGGCAAGAGGCCCAAGA

Coding sequence (CDS)

ATGGGTTGTGTTGCCTCAAAGTTGGAGGAAGAAGAAGAAGTAGTCTCCATCTGTAGAGAAAGAAAACGCCACATAAAGTTAGCTGTTGAGAGAAGGTATGCACTTGCAGAGGCACATAGTAAATATTGTCAAGCTTTATATGCTGTTTCAGCAGCCATTAAGCTCTTTGTAGCTCGCCATTCTTCTCCTTCCCCTTTCCTCATCACATTCCCTCCTACATGTTCTTCTTCTTCTCCTCCCCCTTCAGAAAAAGTGATATCCAATCCAATGTTTCTTCAGCAAAGGCCTTCAGATTCCACCCATGAAGCCATACCCGCCTGCCCTTCATGTGCTTGTTCTTCTTCTTCAACCTCCACAGAGTCCTCCATGGAAGAAAGAGAAGAAGAACCAGTAGAGGAGGAGAGGATTGAACAGGTCCCTGCATATTTCTATATGCAAATGCCACCCCCAATGCCATCCCCTCAGAGGGAGTTTAGTTGGGATTTTTTCAATCCATTTGATACCATGAGGACCGATGTCGTCTCCGGGTATCACCAGAGCTCGGAGGAGGAAGAAGGCATGCCAGAGCTGGAGGAAGAGACAGTAGAGAAAAACCAGAGAGTTGAGGATATTGGAGAGAAGGAAGAAGAGAGTCAGTTGAAGAAGAAGGAGCTAAGAGTCATTGATACACCTGTGGAAGGAAGGGAACTTTTGGAAGCATTGCAGGATGTTGAGGATTATTTCATTAGGGCTTATGATTCTGGAATTGATGTTTCTAGGATGTTAGAGGCCAACAAAATTCAGCTTCAATCTGGTTTAGAAGAAATAAAAGAGAACTCGACAAAACTCATCCAAGCGATAACTTGGCACCGGTCTGTTTCGGGCAAGACTTCATCATGTAAGAGTCTAGTAGCATCCAGTTCGAAAGGTTCTTCGCCATGGACGGAGTTTAGTAATGAGCTGTTTGATGATTATGACATAATGGACTCAGGAAGCCATTCGTCGACTCTTGGACGGTTATATGCTTGGGAGAAGAAACTCTACGAAGAGGTCAAGGCTGGGGACAGCATGCACAAACTGTATGAGAAAAGGTGTTCACGACTAAGAAACCAGGACGTTAAAGGGAGCAATGGAATGACAACTGATAAAACTCGGGTTATGGTTAAAGATTTATATGCCAGGATCTTGGTCGCCATTCGAAGTGCTGAATCAATCTCGACAAGAATTGTGAATCTAAGAGATGACGAGCTACAACCTCAGATCATAGAGCTATTAAAAGGCTTAACAAGAACTTGGAAAATCATGTTGGAAGTTCATGAAACCCAGAAAAAGATTATCCTTGAAGTGAAGACTTATTCCTGCCATTCCTATCTGAAGTTCTGTAACGAATCTCACCGTCTTGCAACTCTTCAGCTTGGAGCTGAGCTTCAGAATTGGCGTTCGTGTTTTTCAAAATATGTTGAATCACAGAAGGCTTATGTTGAAGCTCTCCATGGATGGCTGACCAAGTTTGTAGTGCCTGAGGTTGAGTTCTGTTCTCAGGGTCGGACTTCCGCTGTGCCTTATGGGCTGCACGGACCTCCATTGCTTTCAATCTGCCGTGATTGGCTATCATCCATGGAAAAATTACCAGATAAACCAGTAGCCTTTGCATTGAAAAGCTTTACAAAGGATATGAAGGCTCTATCAGATAAACAGATGGAGGAGCTGCAGCAAAAGAGAAGAGTTGAAAGCTTGGCAAAGGAACTCGACCGACGGATACTGTCATTCCAGAAAACAGAGAACAAGTTCCTCGAGTTCAACTTTACCGAGACGAAATCCGAGTTGGAAGTGGAAAATCAGAATGAGTACTTGACAGAAAAGAAAGATCAGCTGGACATGTTCAGGAAGAAGCTTGATTTGGAGAAGGAAAAACACAATAACTGCATTCAAGAAGCACAGAGGATTACATTAAATGGAATACAAACAGGGTTTTCCTCAGTTTTTGATTCCTTATCAGAGTTCTCCAAGGCTTCACAAAAAATGTACGACCACCTAGTGAATTACAGTGAGAATGCCAATAAATCTAAGAACATTAACTATATCGAGGGTTCCTCCCAAACTGAAGAAAATGTAAGCAGATAA

Protein sequence

MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARHSSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQSSEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR
Homology
BLAST of Carg16161 vs. NCBI nr
Match: KAG7010840.1 (hypothetical protein SDJN02_27636, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1343.2 bits (3475), Expect = 0.0e+00
Identity = 700/700 (100.00%), Postives = 700/700 (100.00%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180

Query: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240
           SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420
           LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG
Sbjct: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 701
           FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 700

BLAST of Carg16161 vs. NCBI nr
Match: KAG6571010.1 (Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 697/699 (99.71%), Postives = 698/699 (99.86%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180

Query: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240
           SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420
           LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG
Sbjct: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKEL+RRILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELERRILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVS 700
           FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEEN S
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENTS 699

BLAST of Carg16161 vs. NCBI nr
Match: XP_022944061.1 (nitrate regulatory gene2 protein-like [Cucurbita moschata])

HSP 1 Score: 1326.6 bits (3432), Expect = 0.0e+00
Identity = 691/700 (98.71%), Postives = 697/700 (99.57%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVV+GYHQS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVAGYHQS 180

Query: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240
           SEEEEG+PELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGIPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGIDVSRML ANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLGANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAG+SMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGNSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420
           LRNQDVKGSNGMTT KTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQI+ELLKG
Sbjct: 361 LRNQDVKGSNGMTTYKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWR CFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRLCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCS+GRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDR+ILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRQILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 701
           FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 700

BLAST of Carg16161 vs. NCBI nr
Match: XP_038902520.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1-like [Benincasa hispida] >XP_038902521.1 protein ALTERED PHOSPHATE STARVATION RESPONSE 1-like [Benincasa hispida])

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 633/728 (86.95%), Postives = 655/728 (89.97%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP CS S  PPSEKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTSTE
Sbjct: 61  SSPSPFLITFPPPCSPS--PPSEKVITNPMFLQQMPSDSTHETIATCPSCV-SSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SS+EEREEE VEEERIEQVP Y YMQMPPPMPSPQREF WDFFNPFDTMRTDVV  Y + 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYLYMQMPPPMPSPQREFGWDFFNPFDTMRTDVVGEYREE 180

Query: 181 S----EEEEGMPELEEETVEK---NQRV------ED---------------IGEKEEESQ 240
                 EEEG+PELEE  VEK   NQRV      ED               +GEKE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEEQNQRVVAVVEEEDVGAFKEQRNGVEMVKVGEKEDEGQ 240

Query: 241 LKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN 300
           +K+K L VIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN
Sbjct: 241 MKQKGLNVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGR 360
           STKLIQAITWHRS SGK SSCKSLVASSSKGSS WTEF NELFDDYD+MDSGSHSSTLGR
Sbjct: 301 STKLIQAITWHRSASGKPSSCKSLVASSSKGSSAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQD+KG NG+TTDKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDIKGENGVTTDKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQI+ELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF S+GRTS 
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSRGRTST 540

Query: 541 VPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           +PYGLHGPPLLSIC DWLSSMEKLPDKPVAFALKSF KDMKALSDKQMEE  QKRRVESL
Sbjct: 541 IPYGLHGPPLLSICHDWLSSMEKLPDKPVAFALKSFAKDMKALSDKQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILSFQKTENKF EFNFTETKSELEVENQNEYLTEKKDQLDMFRKKL+LEKEKH
Sbjct: 601 GKELDRRILSFQKTENKFFEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLELEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIEGSS 701
           NNCIQEAQRITLNGIQ+GFS+VF+SLSEFSKASQKMYDHLVN+SEN +KS+NINYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQSGFSAVFESLSEFSKASQKMYDHLVNFSENTDKSENINYIEGSS 720

BLAST of Carg16161 vs. NCBI nr
Match: XP_011649305.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Cucumis sativus] >KGN61947.1 hypothetical protein Csa_006313 [Cucumis sativus])

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 623/728 (85.58%), Postives = 649/728 (89.15%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP       PPSEKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS++
Sbjct: 61  SSPSPFLITFPP-----PSPPSEKVITNPMFLQQMPSDSTHETIATCPSC-ISSSSTSSQ 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SS+EEREEE VEEERIEQVP YFYMQMPPPMPSPQREF WDFFNPF++MRTDVV  Y + 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGMPELEEETVEK---NQRVE---------------------DIGEKEEESQ 240
                 EEEG+PELEE  VEK   NQRV                       +G+KE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVEVIKVGDKEDEGQ 240

Query: 241 LKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN 300
            K+K L VIDTPVEGRELLEALQDVEDYFIRAYDSG+D+SRMLEANKIQLQSGLEEIKEN
Sbjct: 241 FKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDISRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MDSGSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++TDKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSTDKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQIIELLKGLTR+WKIMLEVHETQKKIILEVKTYSCHSYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCHSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF S+GR SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSRGRASA 540

Query: 541 VPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VPYGLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSD QMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDIQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSELEVENQNEYLTEKKDQLD+F+KKLDLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSELEVENQNEYLTEKKDQLDLFKKKLDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIEGSS 701
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENANKS+NINYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENINYIEGSS 720

BLAST of Carg16161 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 4.7e-57
Identity = 194/715 (27.13%), Postives = 344/715 (48.11%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGC  S++ + +E+VS C+ RKR++K  V+ R  L+ +H+ Y ++L AV +++  F    
Sbjct: 1   MGCCQSRI-DSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHF---S 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           S  +P  +   P   S  PPP             RP        P  P    ++ +T+T 
Sbjct: 61  SKETPLHLHHNPPSPSPPPPP-----------PPRPPPP-----PLSPGSETTTWTTTTT 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SS+            +   P       PPP P P    +WDF++PF       +     S
Sbjct: 121 SSV------------LPPPP-------PPPPPPPPPSSTWDFWDPF-------IPPPPSS 180

Query: 181 SEEEEGMPELEEETVEKNQRVEDIGE---------------KEEESQLKKKELRVIDTPV 240
           SEE     E EEET    +     G                    S   K  +    T  
Sbjct: 181 SEE-----EWEEETTTATRTATGTGSDAAVTTAPTTATPQASSVVSGFSKDTMTTTTTGS 240

Query: 241 E--------GRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLI 300
           E        G++L+E +++V++YF++A DSG  +S +LE     + + + +   +S    
Sbjct: 241 ELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLE-----ISTSITDFSGHSK--- 300

Query: 301 QAITWHRSVSGKTSSCKSLVASSSKGSSPWTE-FSNELFDDYDIMDS--------GSHSS 360
                    SGK  S  +   + +  +S WT  F+     +Y             GSHSS
Sbjct: 301 ---------SGKMYSSSNYECNLNP-TSFWTRGFAPSKLSEYRNAGGVIGGNCIVGSHSS 360

Query: 361 TLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARI 420
           T+ RLYAWEKKLY+EVK  +S+   +EK+  ++R  ++K +  + T+K +  V+ L +++
Sbjct: 361 TVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEKLESQL 420

Query: 421 LVAIRSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCH 480
            V+ ++ +S S  I+ LR+ EL PQ++EL+KGL   W+ M E H+ Q  I+ ++K  +  
Sbjct: 421 SVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLKYLNTI 480

Query: 481 SYLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQG 540
              +  +E HR +TLQL  E+Q W   F   V++Q+ Y+++L GWL   +        Q 
Sbjct: 481 PSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLRLSLF-------QF 540

Query: 541 RTSAVPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRR 600
             + +    +   + S C +W  +++++PDK  +  +KSF   +  +  +Q +E +QK+R
Sbjct: 541 SKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKKR 600

Query: 601 VESLAKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLE 660
            ES+ K+ +++  S +  E+K+  ++  E++ +  V        EK+ +++M + K + E
Sbjct: 601 TESMLKDFEKKSASLRALESKYSPYSVPESRKKNPV-------IEKRVKVEMLKGKAEEE 632

Query: 661 KEKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSK 684
           K KH   +   + +TLN +Q GF  VF ++  FS    + ++ + N +++  + +
Sbjct: 661 KSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQ 632

BLAST of Carg16161 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 7.0e-53
Identity = 213/777 (27.41%), Postives = 349/777 (44.92%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGC ASK+ E+E+ V  C+ER+RH+K AV  R  LA AH+ Y ++L   +AA+  F   H
Sbjct: 1   MGCTASKV-EQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGH 60

Query: 61  SS------PSPFLITF------------PP--TCSSSSPPPSEKVISNPMFLQQRPSDST 120
            S       +P L+T             PP  T SSS PPP+  +  +       P   +
Sbjct: 61  PSLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPTQS 120

Query: 121 HEAIPACPSCACSS-----------SSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPP 180
           H+  P     A              S +S  S       +PV         A+ +    P
Sbjct: 121 HQPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARSSFRKPVVGTP-SSSSAWDWENFYP 180

Query: 181 PMPSPQREFSWDFFNPFDTMRTDV--VSGYHQSSEEEEGMPELEEETVEKNQRVEDIGEK 240
           P P P  EF       FD  + D+   +   +  EEE+    L    +++   V+D  ++
Sbjct: 181 PSP-PDSEF-------FDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDDDE 240

Query: 241 EEE-------------------SQLKKKE------------------------------- 300
            EE                   S+ + +E                               
Sbjct: 241 REEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAAPLP 300

Query: 301 --LRVIDTPVEG-------------------RELLEALQDVEDYFIRAYDSGIDVSRMLE 360
             LR  D   E                    R L E +  +E+YF++A ++G  VS +LE
Sbjct: 301 LPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEYFVKAAEAGNGVSELLE 360

Query: 361 ANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFD 420
           A++ QL     ++K+          +H   +   SS  S   S    +  +   +N L  
Sbjct: 361 ASRAQLDRNFRQLKKT--------VYHS--NSLLSSLSSTWTSKPPLAVRYKLDTNAL-- 420

Query: 421 DYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDK 480
           + + M+  SH STL RL AWEKKLY+EVKA +S+   +EK+ S L++ + +G +    DK
Sbjct: 421 EMESMEGKSHGSTLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDK 480

Query: 481 TRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQK 540
           T+  +  L + I+V  ++A + S+ IV +RD+EL PQ++EL   L   W+ M   HE Q 
Sbjct: 481 TKASINKLQSLIIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQN 540

Query: 541 KIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGW--L 600
           +I+ +V+    +S  +  ++ HRLAT  L A +  W S F++ ++ Q+ Y+ AL+GW  L
Sbjct: 541 EIVQQVRGLVDNSMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLKL 600

Query: 601 TKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKA 660
           T F V +     +  TS +        L + C +W  ++++LPD   + A+KSF   +  
Sbjct: 601 TLFQV-DSNIPQEAYTSLI-----SRELTTFCDEWKQALDRLPDASASEAIKSFVNVVHV 660

Query: 661 LSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEF---------NFTETKSELEVE 663
           +  KQ EE++ K+R E+ +KEL+++  S +  E K+ +                 E    
Sbjct: 661 IYTKQAEEMKIKKRTETYSKELEKKTNSLRAIEKKYYQSYSMVGLGLPGSGRDGIESHSF 720

BLAST of Carg16161 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.9e-45
Identity = 211/805 (26.21%), Postives = 349/805 (43.35%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVA-- 60
           MGC ASKL + E+ V  C++R+R +K AV  R+ LA AH+ YC++L    +A+  F +  
Sbjct: 1   MGCAASKL-DNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGE 60

Query: 61  ----RHSSPSPFLITFPPTCSSSSP----PPSEKVISNPMFLQQRPSDSTHEAIPACPSC 120
                  +P+ FL T PP  S  SP    PP       P  +   PS S   A    PS 
Sbjct: 61  PLSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYP-PSTSPSVASSKQPSV 120

Query: 121 ACSSSSTSTESSMEER------EEEPVEEERIEQ-------VPAYF----YMQMP----- 180
             +SS+   +   + R      E  P    R E+        P+ +    Y   P     
Sbjct: 121 MSTSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASS 180

Query: 181 --------PPMPSPQREF--------------------------SWDFFN-------PFD 240
                   PP P P  EF                           +DFF+        F+
Sbjct: 181 VWNWENFYPPSP-PDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFE 240

Query: 241 TMRTDVVSGYHQSSEE---------------EEGMPELEEETVEKNQRVEDIGEKE---- 300
           +MR  V        EE                      EEE  +  + + ++G +     
Sbjct: 241 SMRNQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGS 300

Query: 301 ------------------------EESQLKKKELRVIDT--------------PVEGREL 360
                                   E+S+  K +   I +               V  R+L
Sbjct: 301 TVRSNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRHRDL 360

Query: 361 LEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKE---NSTKLIQAITWHRSVS 420
            E +  +++ F +A  SG  VS+MLE  + +L     ++K+   +S+ L+  ++      
Sbjct: 361 KEIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSSSLLSNLS------ 420

Query: 421 GKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAG 480
             T + K  +A   +  +   +  N          S S  STL RL AWEKKLYEE+KA 
Sbjct: 421 -STWTSKPPLAVKYRIDTTALDQPN---------SSKSLCSTLDRLLAWEKKLYEEIKAR 480

Query: 481 DSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRD 540
           +     +EK+ S+L++Q+ KG +    DKT+  +  L + I+V  ++  + ST I+ LRD
Sbjct: 481 EGFKIEHEKKLSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRD 540

Query: 541 DELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYL-KFCNESHRLATLQLG 600
            +L PQ++EL  G    WK M + HETQ  I+ +V+     S   +  +E HR AT  L 
Sbjct: 541 TDLVPQLVELCHGFMYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLE 600

Query: 601 AELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPL--LS 660
           + + +W S FS  ++ Q+ ++ ++H W    ++P    C +   +      H  PL   +
Sbjct: 601 SAVSSWHSSFSSLIKFQRDFIHSVHAWFKLTLLP---VCQEDAANH-----HKEPLDAYA 660

Query: 661 ICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQ 663
            C +W  +++++PD   + A+KSF   +  +S KQ +E + K+R ES +KEL+++  S +
Sbjct: 661 FCDEWKLALDRIPDTVASEAIKSFINVVHVISAKQADEHKIKKRTESASKELEKKASSVR 720

BLAST of Carg16161 vs. ExPASy TrEMBL
Match: A0A6J1FTE2 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448612 PE=4 SV=1)

HSP 1 Score: 1326.6 bits (3432), Expect = 0.0e+00
Identity = 691/700 (98.71%), Postives = 697/700 (99.57%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVV+GYHQS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVAGYHQS 180

Query: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240
           SEEEEG+PELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGIPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGIDVSRML ANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLGANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAG+SMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGNSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420
           LRNQDVKGSNGMTT KTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQI+ELLKG
Sbjct: 361 LRNQDVKGSNGMTTYKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWR CFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRLCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCS+GRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDR+ILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRQILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 701
           FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 700

BLAST of Carg16161 vs. ExPASy TrEMBL
Match: A0A0A0LME9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G271390 PE=4 SV=1)

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 623/728 (85.58%), Postives = 649/728 (89.15%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP       PPSEKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS++
Sbjct: 61  SSPSPFLITFPP-----PSPPSEKVITNPMFLQQMPSDSTHETIATCPSC-ISSSSTSSQ 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SS+EEREEE VEEERIEQVP YFYMQMPPPMPSPQREF WDFFNPF++MRTDVV  Y + 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGMPELEEETVEK---NQRVE---------------------DIGEKEEESQ 240
                 EEEG+PELEE  VEK   NQRV                       +G+KE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVEVIKVGDKEDEGQ 240

Query: 241 LKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN 300
            K+K L VIDTPVEGRELLEALQDVEDYFIRAYDSG+D+SRMLEANKIQLQSGLEEIKEN
Sbjct: 241 FKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDISRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MDSGSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++TDKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSTDKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQIIELLKGLTR+WKIMLEVHETQKKIILEVKTYSCHSYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCHSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF S+GR SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSRGRASA 540

Query: 541 VPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VPYGLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSD QMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDIQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSELEVENQNEYLTEKKDQLD+F+KKLDLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSELEVENQNEYLTEKKDQLDLFKKKLDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIEGSS 701
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENANKS+NINYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENINYIEGSS 720

BLAST of Carg16161 vs. ExPASy TrEMBL
Match: A0A5D3BI93 (DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00980 PE=4 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 624/728 (85.71%), Postives = 648/728 (89.01%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP      P P EKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS+E
Sbjct: 61  SSPSPFLITFPP------PSPPEKVITNPMFLQQMPSDSTHETIATCPSC-ISSSSTSSE 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SS+EEREEE VEEERIEQVP YFYMQMPPPMPSPQREF WDFFNPF++MRTDVV  Y + 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGMPELEEETVEK---NQRVE---------------------DIGEKEEESQ 240
                 EEEG+PELEE  VEK   NQRV                       +GEKE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVDVIKVGEKEDEGQ 240

Query: 241 LKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN 300
           LK+K L VIDTPVEGRELLEALQDVEDYFIRAYDSG+DVSRMLEANKIQLQSGLEEIKEN
Sbjct: 241 LKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDVSRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MDSGSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++ DKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSADKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQIIELLKGLTR+WKIMLEVHETQKKIILEVKTYSC+SYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCNSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF SQGR SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSQGRASA 540

Query: 541 VPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VPYGLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSDKQMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDKQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSE EVENQNEYLTEKKDQLD+FRKK+DLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSEFEVENQNEYLTEKKDQLDLFRKKVDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIEGSS 701
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENANKS+N+NYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENMNYIEGSS 720

BLAST of Carg16161 vs. ExPASy TrEMBL
Match: A0A1S3C7H0 (uncharacterized protein LOC103497347 OS=Cucumis melo OX=3656 GN=LOC103497347 PE=4 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 624/728 (85.71%), Postives = 648/728 (89.01%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEEEVVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP      P P EKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS+E
Sbjct: 61  SSPSPFLITFPP------PSPPEKVITNPMFLQQMPSDSTHETIATCPSC-ISSSSTSSE 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SS+EEREEE VEEERIEQVP YFYMQMPPPMPSPQREF WDFFNPF++MRTDVV  Y + 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGMPELEEETVEK---NQRVE---------------------DIGEKEEESQ 240
                 EEEG+PELEE  VEK   NQRV                       +GEKE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVDVIKVGEKEDEGQ 240

Query: 241 LKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN 300
           LK+K L VIDTPVEGRELLEALQDVEDYFIRAYDSG+DVSRMLEANKIQLQSGLEEIKEN
Sbjct: 241 LKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDVSRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MDSGSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++ DKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSADKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQIIELLKGLTR+WKIMLEVHETQKKIILEVKTYSC+SYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCNSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF SQGR SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSQGRASA 540

Query: 541 VPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VPYGLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSDKQMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDKQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSE EVENQNEYLTEKKDQLD+FRKK+DLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSEFEVENQNEYLTEKKDQLDLFRKKVDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIEGSS 701
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENANKS+N+NYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENMNYIEGSS 720

BLAST of Carg16161 vs. ExPASy TrEMBL
Match: A0A6J1JI88 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111484590 PE=4 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 593/611 (97.05%), Postives = 601/611 (98.36%), Query Frame = 0

Query: 90  MFLQQRPSDSTHEAIPACPSCACSSSSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPP 149
           MFLQQRPSDSTHEAIPACPSCAC SSSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPP
Sbjct: 1   MFLQQRPSDSTHEAIPACPSCACCSSSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPP 60

Query: 150 PMPSPQREFSWDFFNPFDTMRTDVVSGYHQSSEEEEGMPELEEETVEKNQRVEDIGEKEE 209
           PMPSPQREFSWDFFNPFDTMRTDVV+GY QSSEEEEG+PELEEETVEKNQRVED+GEKEE
Sbjct: 61  PMPSPQREFSWDFFNPFDTMRTDVVAGYRQSSEEEEGIPELEEETVEKNQRVEDVGEKEE 120

Query: 210 ESQLKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEI 269
           E+QL KK L+VIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEI
Sbjct: 121 ENQL-KKGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEI 180

Query: 270 KENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSST 329
           KENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSST
Sbjct: 181 KENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSST 240

Query: 330 LGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARIL 389
           LGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLR+QDVKGSNGMTTDKTRVMVKDLYARIL
Sbjct: 241 LGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRSQDVKGSNGMTTDKTRVMVKDLYARIL 300

Query: 390 VAIRSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHS 449
           VAIRSAESISTRIVNLRDDELQPQI+ELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHS
Sbjct: 301 VAIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHS 360

Query: 450 YLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQGR 509
           YLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPE EFCS+GR
Sbjct: 361 YLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEDEFCSRGR 420

Query: 510 TSAVPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRV 569
           TS VPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRV
Sbjct: 421 TSTVPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRV 480

Query: 570 ESLAKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEK 629
           ESLAKELDRRILSFQKTENKF EFN TE KSE EVENQNEYLTEKKDQLDMFRKKLDLEK
Sbjct: 481 ESLAKELDRRILSFQKTENKFFEFNSTELKSESEVENQNEYLTEKKDQLDMFRKKLDLEK 540

Query: 630 EKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIE 689
           EKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIE
Sbjct: 541 EKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIE 600

Query: 690 GSSQTEENVSR 701
           GSSQTEENVSR
Sbjct: 601 GSSQTEENVSR 610

BLAST of Carg16161 vs. TAIR 10
Match: AT4G39790.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 236.1 bits (601), Expect = 8.5e-62
Identity = 204/657 (31.05%), Postives = 317/657 (48.25%), Query Frame = 0

Query: 1   MGCVASK--LEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLF-- 60
           MGC  SK  + ++ E + +C+ERKR +K A++ R ALA AH  Y ++L  + A ++ +  
Sbjct: 9   MGCSNSKASMNKKNEPLHLCKERKRFVKQAMDSRCALAAAHVSYIRSLRNIGACLRQYAE 68

Query: 61  --VARHSSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSS 120
              A  SSPS    T P    S +    +  + +P+     P+ +        P    S 
Sbjct: 69  AETAHESSPS-LTATEPEKSPSHNSSYPDDSVDSPLSHNSNPNPNPK------PLFNLSY 128

Query: 121 SSTSTESSMEEREEEPV---EEERIEQVPAYFYMQMPPPMPSPQR--EFSWDFF---NPF 180
             T T SS       P+   +++    +PA+     PPP P P+R    SWD+F   + F
Sbjct: 129 MKTETASSTVTFTINPLSDGDDDLEVTMPAF----SPPPPPRPRRPETSSWDYFDTCDDF 188

Query: 181 DTMR---------------------TDVVSGYHQSSEEEEGMPELEEETVEKNQRVEDIG 240
           D+ R                       + S  + +    E + +   +T ++ Q  ED  
Sbjct: 189 DSFRFVGLSEQTEIDSECDAAVIGLEKITSQGNVAKSGSETLQDSSFKTKQRKQSCEDND 248

Query: 241 EKEEESQLKKKELRVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSG 300
           E+E+ S+               ++ + +++D+E  F RA +SG +VSRMLE NKI++   
Sbjct: 249 EREDPSEF---------ITHRAKDFVSSMKDIEHKFFRASESGREVSRMLEVNKIRVGFA 308

Query: 301 LEEIKENSTKLIQA--------------------------ITWHRSVSGKTSSCKSLVAS 360
               K NS   + A                          I W R+ S ++S+ ++ +  
Sbjct: 309 DMTGKGNSIAFLAALKRACCRGKSYSPVSQEPLSHQVTKVIVWKRTSSSRSSTSRNPLIQ 368

Query: 361 SSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCS 420
           +SK      E  ++  +++  M SGSHSS+L RLYAWE+KLY+EVKA + + K Y+++C 
Sbjct: 369 TSKEDHD-DESGSDFIEEF-CMISGSHSSSLDRLYAWERKLYDEVKASEMIRKEYDRKCE 428

Query: 421 RLRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLK 480
           +LRNQ  K  +  + DKTR   KDL++RI VAI+S ESIS RI  +RDDEL PQ++E L+
Sbjct: 429 QLRNQFAKDHSAKSMDKTRAAAKDLHSRIRVAIQSVESISKRIERIRDDELHPQLLEFLQ 488

Query: 481 GLTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNES--HRLATLQLGAELQNWRSCFS 540
           GL R WK MLE H TQ   I     Y C    K  +ES   R    +L  E + +   F 
Sbjct: 489 GLIRMWKAMLECHHTQ--YITISLAYHCRHSSKTAHESVLKRRILAELLEETECFGLSFV 548

Query: 541 KYVESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLP 593
             V S  +YVEAL+GWL   V+   E  ++ R    P  +  PP+  +CRDW + ++ LP
Sbjct: 549 DLVHSMASYVEALNGWLHNCVLLPQERSTRNRRPWSPRRVLAPPIFVLCRDWSAGIKTLP 608

BLAST of Carg16161 vs. TAIR 10
Match: AT4G30130.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 229.2 bits (583), Expect = 1.0e-59
Identity = 213/662 (32.18%), Postives = 317/662 (47.89%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGC  SK  +++E V IC++RKR IK AVE R   A  H  Y Q+L  VS A++ ++   
Sbjct: 1   MGCSHSKF-DDDEAVQICKDRKRFIKQAVEHRTGFASGHIAYIQSLRKVSDALREYI-EG 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDS--THEAIPACPSCACSSSSTS 120
             P  F++    TC +    P +++ S+  F++  P       EA       +   +S S
Sbjct: 61  DEPHEFML---DTCVT----PVKRMSSSGGFIEISPPSKMVQSEAESKLNVNSYLMASGS 120

Query: 121 TESSMEEREEEPVEEERIEQVPA--YFYMQMPP----------PMPSPQREFSWDFF-NP 180
               +EE+     E  ++E   A  +F M M            P PSPQ    WDFF NP
Sbjct: 121 RPVRVEEKPPRSPETFQVETYGADSFFGMNMNMNSPGLGSHNIPPPSPQNS-QWDFFWNP 180

Query: 181 FDTMRTDVVSGYHQSS-----------EEEEGMPELEEETVEK----------------- 240
           F  +     S  +Q+             EEEG+P+LEE+   K                 
Sbjct: 181 FSALDQYGYSYDNQNGMDDDMRRLRRVREEEGIPDLEEDEYVKFEDHHNMKATEDFNGGK 240

Query: 241 ---NQRVEDIGEKEEESQLK-----------KKELRVID-------------TPVEGR-- 300
                +VE + E+  +S  K            +E R ++             T  +G+  
Sbjct: 241 MYQEDKVEHVNEEFTDSGCKIENESDKNCNGTQERRSLEVSRGGTTGHVVGVTSDDGKGE 300

Query: 301 -------------ELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTK 360
                         + E ++D+ED F     +G +VS +LEA+++Q  S  E    ++  
Sbjct: 301 TPGFTVYLNRRPTSMAEVIKDLEDQFAIICTAGKEVSGLLEASRVQYTSSNE---LSAMT 360

Query: 361 LIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEF-SNELFDDYDIMDSGSHSSTLGRLY 420
           ++  +   RS     SS  S    SS G S  +EF S+  F +   M SGSH STL RLY
Sbjct: 361 MLNPVALFRSGGSSRSSSSSRFLISSSGGSRASEFESSSEFSEESCMLSGSHQSTLDRLY 420

Query: 421 AWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRS 480
           AWEKKLY+EVK+GD +   YEK+C  LRNQDVKG++    DKTR  ++DL+ +I V+I S
Sbjct: 421 AWEKKLYDEVKSGDRIRIAYEKKCLVLRNQDVKGADSSAVDKTRATIRDLHTQIKVSIHS 480

Query: 481 AESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVK----TYSCHSY 540
            ESIS RI  LRD EL PQ++EL++GL + WK+M E H+ QK+ + E K    T   + +
Sbjct: 481 IESISERIETLRDQELLPQLLELVQGLAQMWKVMAECHQIQKRTLDEAKLLLATTPSNRH 540

Query: 541 LKFCN------ESHRLA--TLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKF--VVP 563
            K          S RLA   L L  +L+NWR+CF  ++ SQ++Y+ +L GWL +     P
Sbjct: 541 KKQQQTSLPEINSQRLARSALHLVVQLRNWRACFQAWITSQRSYILSLTGWLLRCFRCDP 600

BLAST of Carg16161 vs. TAIR 10
Match: AT3G51290.2 (Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) )

HSP 1 Score: 226.9 bits (577), Expect = 5.1e-59
Identity = 195/720 (27.08%), Postives = 347/720 (48.19%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGC  S++ + +E+VS C+ RKR++K  V+ R  L+ +H+ Y ++L AV +++  F    
Sbjct: 1   MGCCQSRI-DSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHF---S 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           S  +P  +   P   S  PPP             RP        P  P    ++ +T+T 
Sbjct: 61  SKETPLHLHHNPPSPSPPPPP-----------PPRPPPP-----PLSPGSETTTWTTTTT 120

Query: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180
           SS+            +   P       PPP P P    +WDF++PF       +     S
Sbjct: 121 SSV------------LPPPP-------PPPPPPPPPSSTWDFWDPF-------IPPPPSS 180

Query: 181 SEEEEGMPELEEETVEKNQRVEDIGE---------------KEEESQLKKKELRVIDTPV 240
           SEE     E EEET    +     G                    S   K  +    T  
Sbjct: 181 SEE-----EWEEETTTATRTATGTGSDAAVTTAPTTATPQASSVVSGFSKDTMTTTTTGS 240

Query: 241 E--------GRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLI 300
           E        G++L+E +++V++YF++A DSG  +S +LE     + + + +   +S    
Sbjct: 241 ELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLE-----ISTSITDFSGHSK--- 300

Query: 301 QAITWHRSVSGKTSSCKSLVASSSKGSSPWTE-FSNELFDDYDIMDS--------GSHSS 360
                    SGK  S  +   + +  +S WT  F+     +Y             GSHSS
Sbjct: 301 ---------SGKMYSSSNYECNLNP-TSFWTRGFAPSKLSEYRNAGGVIGGNCIVGSHSS 360

Query: 361 TLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMVKDLYARI 420
           T+ RLYAWEKKLY+EVK  +S+   +EK+  ++R  ++K +  + T+K +  V+ L +++
Sbjct: 361 TVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEKLESQL 420

Query: 421 LVAIRSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILEVKTYSCH 480
            V+ ++ +S S  I+ LR+ EL PQ++EL+KGL   W+ M E H+ Q  I+ ++K  +  
Sbjct: 421 SVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLKYLNTI 480

Query: 481 SYLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSQG 540
              +  +E HR +TLQL  E+Q W   F   V++Q+ Y+++L GWL   +        Q 
Sbjct: 481 PSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLRLSLF-------QF 540

Query: 541 RTSAVPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRR 600
             + +    +   + S C +W  +++++PDK  +  +KSF   +  +  +Q +E +QK+R
Sbjct: 541 SKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKKR 600

Query: 601 VESLAKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLE 660
            ES+ K+ +++  S +  E+K+  ++  E++ +  V        EK+ +++M + K + E
Sbjct: 601 TESMLKDFEKKSASLRALESKYSPYSVPESRKKNPV-------IEKRVKVEMLKGKAEEE 637

Query: 661 KEKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYI 689
           K KH   +   + +TLN +Q GF  VF ++  FS    + ++ + N +++  + +  +Y+
Sbjct: 661 KSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQEEDYV 637

BLAST of Carg16161 vs. TAIR 10
Match: AT2G27090.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 216.5 bits (550), Expect = 7.0e-56
Identity = 207/731 (28.32%), Postives = 335/731 (45.83%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIK------ 60
           MG   S++ +E++ + +CRERK+ ++ A++ R  LA AH  Y Q+L +   A++      
Sbjct: 1   MGASTSRI-DEDKALQLCRERKKFVQQALDGRCLLAAAHVSYVQSLKSTGTALRKFSETE 60

Query: 61  ------LFVARHSSPSPFLITF----------PPTCSSS-----SPPPSEKVIS----NP 120
                 L+ +  ++P   L             PP  S S     SPPPS    S    N 
Sbjct: 61  VPVESSLYTSTSATPEQPLALIEKSVSHLSYSPPPASHSHHDTYSPPPSPPSTSPFQVNH 120

Query: 121 MFLQQRPSDSTHEAIPACPSCACSSSSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPP 180
           M  +   S    E  P       +SSS     S+E+ E  P EE             MPP
Sbjct: 121 MKFKGFSSKKVEEKPPVSIIATVTSSSIPPSRSIEKMESTPFEES----------SSMPP 180

Query: 181 PMPSPQREFSWDFF---NPFDTM--RTDVVSGY-HQSSEEEEGMPELEEE---------- 240
             P       WD+F   +P D     + V +G+  +S +EE+G PE E++          
Sbjct: 181 EAP-------WDYFGLSHPIDNQFSSSHVGNGHVSRSVKEEDGTPEEEDDGEDFSFQERE 240

Query: 241 ---------------------------------TVEKNQRVE---DIGEKEEESQLKKKE 300
                                            T+ + + VE      EK +  +L    
Sbjct: 241 ESRDSDDDEFDEPTSDTLVRSFENFNRVRRDHSTLPQREGVESDSSDAEKSKTPELSPPV 300

Query: 301 LRVIDTPVE--------------GRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQ 360
             ++ TPV                R+ L +++++E  F++A ++G +V RMLEANK+  +
Sbjct: 301 TPLVATPVNKTPNKGDHTENKLPPRDFLSSMKEIELLFVKASETGKEVPRMLEANKLHFR 360

Query: 361 ---------SGLEEI---------------KENSTKLIQAITWHRSVSGKTSSCKSLVAS 420
                    SG   +               +E +   ++ +TWHR+ S ++SS ++ +  
Sbjct: 361 PIVPSKESGSGASSLFKTCLSCGEDPKDVPEEPAQNSVKYLTWHRTESSRSSSSRNPLGG 420

Query: 421 SSKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCS 480
            +  S    E ++ LF++   M +GSH+STL RLYAWE+KLY+EVK   ++ + Y+++C 
Sbjct: 421 MN--SDDVEELNSNLFENI-CMIAGSHASTLDRLYAWERKLYDEVKGSQTVRREYDEKCR 480

Query: 481 RLRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLK 540
            LR  + +G      DKTR +VKDL++RI VAI   +SIS RI  LRD+ELQPQ+ EL++
Sbjct: 481 ILRELESEGKGSQRIDKTRAVVKDLHSRIRVAIHRIDSISRRIEELRDNELQPQLEELIE 540

Query: 541 GLTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKY 600
           GL+R W++MLE H+ Q ++I +      +  L   +E HR  T  L  EL    S F+K+
Sbjct: 541 GLSRMWEVMLECHKVQFQLI-KACYRGGNIKLNMQSELHRQVTSHLEDELCALASSFTKW 600

Query: 601 VESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDK 611
           +  QK+Y++A++ WL K V        + R        +GPP+ + C  WL  +E LP K
Sbjct: 601 ITGQKSYIQAINEWLVKCVALPQRSKRKRRAPQPSLRNYGPPIYATCGIWLEKLEVLPTK 660

BLAST of Carg16161 vs. TAIR 10
Match: AT2G19090.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 215.3 bits (547), Expect = 1.6e-55
Identity = 216/737 (29.31%), Postives = 325/737 (44.10%), Query Frame = 0

Query: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGC  SKL ++EE V IC++RKR IK A+E R   A  H  Y  +L  VS A+  F+ + 
Sbjct: 1   MGCSHSKL-DDEEAVQICKDRKRFIKQAIEHRTKFASGHIAYIHSLRKVSDALHDFILQG 60

Query: 61  SSPSPFLIT------------FPPTCSSSSPPPSEKVIS------NPMFLQQRP-SDSTH 120
            + + F+ T             P     SS   S + IS       P  +Q RP S+   
Sbjct: 61  DNNNEFVPTLCQDSFVTPVKRMPQRRRRSSRSNSGEFISISPSSIPPKMIQGRPRSNVKA 120

Query: 121 EAIPACPSCACSSSSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPP------------ 180
             + A  S        S E+   E    P    +  +   +F M M              
Sbjct: 121 NYLMANRSRPVRVEQRSPETFRVETFSPPSSSNQYGESDGFFGMNMNMNTSASTSSSFWN 180

Query: 181 --------------PMPSPQREFSWDFF-NPFDTMRTDVVSGYHQSS------------- 240
                         P PSPQ    WDFF NPF ++     + Y + S             
Sbjct: 181 PLSSPEQRLSSHNIPPPSPQNS-QWDFFWNPFSSLDYYGYNSYDRGSVDSRSGIDDEIRG 240

Query: 241 ----EEEEGMPELEEE----------------------TVEKNQ------------RVED 300
                EEEG+P+LEE+                        E+N+            +VED
Sbjct: 241 LRRVREEEGIPDLEEDDEPHKPEPVVNVRFQNHNPNPKATEENRGKVDKSCCNEEVKVED 300

Query: 301 IGEKEEE----------------------------SQLKKK------------------- 360
           + E E+E                            +Q ++K                   
Sbjct: 301 VDEDEDEDGDEDDDEFTDSGCETENEGDEKCVAPPTQEQRKAEVCRGGEATGNVVGVGKV 360

Query: 361 -ELRVI-----DTPVEG---------RELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQ 420
            E++ +     D    G           + E ++D+ED F    D+  +VS +LEA + Q
Sbjct: 361 QEMKNVVGVRDDAKTTGFTVYVNRRPTSMAEVIKDLEDQFTTICDAAKEVSGLLEAGRAQ 420

Query: 421 LQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIM 480
             S   +   ++ K++  +   RS S ++SS + L+ SS       +E  +++ D+   M
Sbjct: 421 YTSSFND--HSARKMLNPVALFRSGSSRSSSSRFLITSSGGSRESGSESRSDVSDE-SCM 480

Query: 481 DSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVMV 540
            SGSH +TL RL+AWEKKLY+EV++G+ + + YEK+C +LRNQDVKG + +  DKTR  +
Sbjct: 481 ISGSHQTTLDRLFAWEKKLYDEVRSGERVRRAYEKKCMQLRNQDVKGDDPLAVDKTRATI 540

Query: 541 KDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKGLTRTWKIMLEVHETQKKIILE 563
           +DL  +I V+I S ESIS RI  LRD EL PQ++EL++GLTR WK+M E H+ QK+ + E
Sbjct: 541 RDLDTQIKVSIHSIESISKRIETLRDQELLPQLLELVEGLTRMWKVMAESHQIQKRTLDE 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7010840.10.0e+00100.00hypothetical protein SDJN02_27636, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6571010.10.0e+0099.71Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022944061.10.0e+0098.71nitrate regulatory gene2 protein-like [Cucurbita moschata][more]
XP_038902520.10.0e+0086.95protein ALTERED PHOSPHATE STARVATION RESPONSE 1-like [Benincasa hispida] >XP_038... [more]
XP_011649305.10.0e+0085.58protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Cucumis sativus] >KGN61947.1 hy... [more]
Match NameE-valueIdentityDescription
A0A178VBJ04.7e-5727.13Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9AQW17.0e-5327.41Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
Q93YU81.9e-4526.21Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1FTE20.0e+0098.71nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448... [more]
A0A0A0LME90.0e+0085.58Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G271390 PE=4 SV=1[more]
A0A5D3BI930.0e+0085.71DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis mel... [more]
A0A1S3C7H00.0e+0085.71uncharacterized protein LOC103497347 OS=Cucumis melo OX=3656 GN=LOC103497347 PE=... [more]
A0A6J1JI880.0e+0097.05nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC11148459... [more]
Match NameE-valueIdentityDescription
AT4G39790.18.5e-6231.05Protein of unknown function (DUF630 and DUF632) [more]
AT4G30130.11.0e-5932.18Protein of unknown function (DUF630 and DUF632) [more]
AT3G51290.25.1e-5927.08Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) [more]
AT2G27090.17.0e-5628.32Protein of unknown function (DUF630 and DUF632) [more]
AT2G19090.11.6e-5529.31Protein of unknown function (DUF630 and DUF632) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 611..631
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..134
NoneNo IPR availablePANTHERPTHR21450:SF17OS09G0542500 PROTEINcoord: 1..684
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..684
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 230..553
e-value: 6.1E-109
score: 364.2
IPR006868Domain of unknown function DUF630PFAMPF04783DUF630coord: 1..60
e-value: 8.6E-22
score: 77.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16161-RACarg16161-RAmRNA