Carg16124 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16124
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr20: 3399356 .. 3400797 (+)
RNA-Seq Expression	Carg16124
Synteny	Carg16124

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAGTGTGGCAGAAGAGACAGAAAGCTAACCAGGGTTAGAGAACGTGGGTTAGTCTTCTTCAGAGCCGGAGAGGTAGGTTTGGGGTTTGTCGAGCTCCAGAGCTTTACAGGAGGAAGAAGGAAGCTGCTGTGTGTATAAAACCACCCAGAAAGAAAACCCTCTTGTCATTAACAAAACATACAAAAACGGAAACAAACCTCTCAACGATTCTGTGAATCGCGATCATCTCGATCATCTCTTAGCTCTTCCTCTTGTTAATCTCTCTTCTTCTCATCCTTCGCCGGCGAGCCTCACAGATATTGGTATGGTTTCTATAGATTCGGATTATGATCTTGGTTTCATTTGCTGATTTGGGTTCTCGGGGTTTCTGGTTTTGGATTTGATCCGATAGGGGGGGTTCGAACTGGATTGATTTCCCTTTGCATAATCGGTGTTCTTCTGACGATCCCTTTGCATAATCGGTGTTCTTCTGACGATATTTTGATCATTCTTTCAATCTTTTTGGATCGTTTCTGGGTAAATCGAAACTCTGTGTTCTAGAATGTTGGGTTTCTCTCTGTTTCCTTAGCTGTTCTTTCTTCTCTTTCTCCTGCAATGAAATTGTCAGTGGAAGGTTATCTTGATGATTCTATTCCATGTTCTTGGCTCTTGCATCTAAACAGATCTTCTCTCAAGGAAGTTTCTATTACCAGTATATACTTTTCTGCCTTCCTTAATCTTCGTTTTCCTGTTGGGAGCTAGGATCATAGAAGGAAGGAGTACACTCGATTCATTAATCTTTCAGCAGATTTGGCTCTATTTCTATACGATGTTTGATAAGTTGTTTGTGCATTTTCAGATCAGATATCAACAAAAGGATCAATCTACAGAACCGTTTGAACTCAGAACTCAGAAGTGACTTCTTATACATTCTACTGATCTTCTTTGATTCATCTGAGCTGATCCCAGTTCTTCATATTTCAAACAACCTTATGGGTCTGTCCAGCCTTCCTGCTCCTTCTGAAGGAGTACTGGGTGTGATCCTTGTAAATACGGCCCTATCGATATCTATCTTCAAAGGCATAGTTCGGTCGATTCTCCATGTCGTTGGCATCCATCTCTCGTCGCCTACATTACCATCCTCACCAGATTCCATGGAGAATGCTCCCGAATCCATAGAATTCCATCTTAACCCGAATGGAAGTTACATTGAAGAGTTCCGCAGCCGAATCCCAGCAATTCTGTTTAACAAAGTTCACAGCTGCAAATTGCTCGAACATGACTGCTCGGTCTGCCTAACCCAGTTTGCACCTGATTCAGAGATAAACCATTTATCTTGTGGTCATCTTTTCCATAGAGTGTGCTTGGAGAAGTGGCTGGACTACTGGAACCTTACGTGTCCTCTGTGTCGAACTCCGTTCATGCCCGAAGAAGACACAGCTTCCTGCTTCTGGTGA

mRNA sequence

ATGGCAGAGTGTGGCAGAAGAGACAGAAAGCTAACCAGGGTTAGAGAACGTGGGTTAGTCTTCTTCAGAGCCGGAGAGAATGTTGGGTTTCTCTCTGTTTCCTTAGCTGTTCTTTCTTCTCTTTCTCCTGCAATGAAATTATCAGATATCAACAAAAGGATCAATCTACAGAACCGTTTGAACTCAGAACTCAGAAGTGACTTCTTATACATTCTACTGATCTTCTTTGATTCATCTGAGCTGATCCCAGTTCTTCATATTTCAAACAACCTTATGGGTCTGTCCAGCCTTCCTGCTCCTTCTGAAGGAGTACTGGGTGTGATCCTTGTAAATACGGCCCTATCGATATCTATCTTCAAAGGCATAGTTCGGTCGATTCTCCATGTCGTTGGCATCCATCTCTCGTCGCCTACATTACCATCCTCACCAGATTCCATGGAGAATGCTCCCGAATCCATAGAATTCCATCTTAACCCGAATGGAAGTTACATTGAAGAGTTCCGCAGCCGAATCCCAGCAATTCTGTTTAACAAAGTTCACAGCTGCAAATTGCTCGAACATGACTGCTCGGTCTGCCTAACCCAGTTTGCACCTGATTCAGAGATAAACCATTTATCTTGTGGTCATCTTTTCCATAGAGTGTGCTTGGAGAAGTGGCTGGACTACTGGAACCTTACGTGTCCTCTGTGTCGAACTCCGTTCATGCCCGAAGAAGACACAGCTTCCTGCTTCTGGTGA

Coding sequence (CDS)

ATGGCAGAGTGTGGCAGAAGAGACAGAAAGCTAACCAGGGTTAGAGAACGTGGGTTAGTCTTCTTCAGAGCCGGAGAGAATGTTGGGTTTCTCTCTGTTTCCTTAGCTGTTCTTTCTTCTCTTTCTCCTGCAATGAAATTATCAGATATCAACAAAAGGATCAATCTACAGAACCGTTTGAACTCAGAACTCAGAAGTGACTTCTTATACATTCTACTGATCTTCTTTGATTCATCTGAGCTGATCCCAGTTCTTCATATTTCAAACAACCTTATGGGTCTGTCCAGCCTTCCTGCTCCTTCTGAAGGAGTACTGGGTGTGATCCTTGTAAATACGGCCCTATCGATATCTATCTTCAAAGGCATAGTTCGGTCGATTCTCCATGTCGTTGGCATCCATCTCTCGTCGCCTACATTACCATCCTCACCAGATTCCATGGAGAATGCTCCCGAATCCATAGAATTCCATCTTAACCCGAATGGAAGTTACATTGAAGAGTTCCGCAGCCGAATCCCAGCAATTCTGTTTAACAAAGTTCACAGCTGCAAATTGCTCGAACATGACTGCTCGGTCTGCCTAACCCAGTTTGCACCTGATTCAGAGATAAACCATTTATCTTGTGGTCATCTTTTCCATAGAGTGTGCTTGGAGAAGTGGCTGGACTACTGGAACCTTACGTGTCCTCTGTGTCGAACTCCGTTCATGCCCGAAGAAGACACAGCTTCCTGCTTCTGGTGA

Protein sequence

MAECGRRDRKLTRVRERGLVFFRAGENVGFLSVSLAVLSSLSPAMKLSDINKRINLQNRLNSELRSDFLYILLIFFDSSELIPVLHISNNLMGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPESIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLFHRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW

Homology

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BLAST of Carg16124 vs. NCBI nr
Match: KAG7010803.1 (putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 494.2 bits (1271), Expect = 6.5e-136
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MAECGRRDRKLTRVRERGLVFFRAGENVGFLSVSLAVLSSLSPAMKLSDINKRINLQNRL 60
           MAECGRRDRKLTRVRERGLVFFRAGENVGFLSVSLAVLSSLSPAMKLSDINKRINLQNRL
Sbjct: 1   MAECGRRDRKLTRVRERGLVFFRAGENVGFLSVSLAVLSSLSPAMKLSDINKRINLQNRL 60

Query: 61  NSELRSDFLYILLIFFDSSELIPVLHISNNLMGLSSLPAPSEGVLGVILVNTALSISIFK 120
           NSELRSDFLYILLIFFDSSELIPVLHISNNLMGLSSLPAPSEGVLGVILVNTALSISIFK
Sbjct: 61  NSELRSDFLYILLIFFDSSELIPVLHISNNLMGLSSLPAPSEGVLGVILVNTALSISIFK 120

Query: 121 GIVRSILHVVGIHLSSPTLPSSPDSMENAPESIEFHLNPNGSYIEEFRSRIPAILFNKVH 180
           GIVRSILHVVGIHLSSPTLPSSPDSMENAPESIEFHLNPNGSYIEEFRSRIPAILFNKVH
Sbjct: 121 GIVRSILHVVGIHLSSPTLPSSPDSMENAPESIEFHLNPNGSYIEEFRSRIPAILFNKVH 180

Query: 181 SCKLLEHDCSVCLTQFAPDSEINHLSCGHLFHRVCLEKWLDYWNLTCPLCRTPFMPEEDT 240
           SCKLLEHDCSVCLTQFAPDSEINHLSCGHLFHRVCLEKWLDYWNLTCPLCRTPFMPEEDT
Sbjct: 181 SCKLLEHDCSVCLTQFAPDSEINHLSCGHLFHRVCLEKWLDYWNLTCPLCRTPFMPEEDT 240

Query: 241 ASCFW 246
           ASCFW
Sbjct: 241 ASCFW 245

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BLAST of Carg16124 vs. NCBI nr
Match: KAG6570965.1 (putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 326.2 bits (835), Expect = 2.3e-85
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 60

Query: 152 SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 211
           SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF
Sbjct: 61  SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 120

Query: 212 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW
Sbjct: 121 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 154

[Close]

BLAST of Carg16124 vs. NCBI nr
Match: XP_022944133.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata] >XP_022944135.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata])

HSP 1 Score: 326.2 bits (835), Expect = 2.3e-85
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 60

Query: 152 SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 211
           SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF
Sbjct: 61  SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 120

Query: 212 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW
Sbjct: 121 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 154

[Close]

BLAST of Carg16124 vs. NCBI nr
Match: XP_022985804.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima] >XP_022985805.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima] >XP_022985806.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima])

HSP 1 Score: 325.1 bits (832), Expect = 5.2e-85
Identity = 153/154 (99.35%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 60

Query: 152 SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 211
           SIEFHLNPNG+YIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF
Sbjct: 61  SIEFHLNPNGNYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 120

Query: 212 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW
Sbjct: 121 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 154

[Close]

BLAST of Carg16124 vs. NCBI nr
Match: XP_023512749.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo] >XP_023512750.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo] >XP_023512751.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321.6 bits (823), Expect = 5.8e-84
Identity = 152/154 (98.70%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDS+ENA E
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSIENASE 60

Query: 152 SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 211
           SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF
Sbjct: 61  SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 120

Query: 212 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW
Sbjct: 121 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 154

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BLAST of Carg16124 vs. ExPASy Swiss-Prot
Match: Q9SI09 (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=XERICO PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 6.7e-43
Identity = 95/158 (60.13%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLS-SPTLPSSPD-SMENA 151
           MGLSSLP PSEG+L VILVNTALSISI KGIVRS L +VGI LS S + PSS   S EN+
Sbjct: 1   MGLSSLPGPSEGMLCVILVNTALSISIVKGIVRSFLGIVGISLSPSSSSPSSVTVSSENS 60

Query: 152 --PESIEFHLNPNGSYIEEFRSRIPAILFNKVHSC-KLLEHDCSVCLTQFAPDSEINHLS 211
              ES +F +    SY+EEFR+R P + F  +  C K  +++CSVCL++F  DSEIN L 
Sbjct: 61  STSESFDFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLK 120

Query: 212 CGHLFHRVCLEKWLDYWNLTCPLCRTPF--MPEEDTAS 243
           CGHLFH+ CLEKW+DYWN+TCPLCRTP   +PE+   S
Sbjct: 121 CGHLFHKTCLEKWIDYWNITCPLCRTPLVVVPEDHQLS 158

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BLAST of Carg16124 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.2e-13
Identity = 48/140 (34.29%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 101 SEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPESIEFHLNPN 160
           +E  L  + V T   +   + IV SI   +G+        + PD   + P  I    +P 
Sbjct: 8   TEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPD-YTSYPTRIPETRSPF 67

Query: 161 GSYIEEFRSRIPAILFNKV-HSCKLLEHDCSVCLTQFAPDSEINHL-SCGHLFHRVCLEK 220
            + +   R  +P I F ++ +S + L  +C+VCL +F  + EI  L +C H+FHR CL++
Sbjct: 68  SALL--IREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDR 127

Query: 221 WLDYWNLTCPLCRTPFMPEE 239
           W+D+   TCPLCRTPF+P+E
Sbjct: 128 WMDHDQKTCPLCRTPFVPDE 144

[Close]

BLAST of Carg16124 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.0e-10
Identity = 43/140 (30.71%), Postives = 70/140 (50.00%), Query Frame = 0


Query: 109 LVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENA-PESIEFHLNPNGSYIEEF 168
           ++ T   I  F+ +V ++   +G       LPS  D  E +  +     L+ + +   E 
Sbjct: 16  VLKTLYVIGFFRDMVDALCPYIG-------LPSFLDHNETSRSDPTRLALSTSATLANEL 75

Query: 169 RSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHL-SCGHLFHRVCLEKWL-DYWNL 228
              IP + F+ + +    E  C+VCL+ F  D +I  L  CGH+FH  CL++W+ D   +
Sbjct: 76  ---IPVVRFSDLLTDP--EDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDCNKI 135

Query: 229 TCPLCRTPFMPEEDTASCFW 246
           TCP+CR  F+PEE +    W
Sbjct: 136 TCPICRNRFLPEEKSTPFDW 143

[Close]

BLAST of Carg16124 vs. ExPASy Swiss-Prot
Match: Q9ZU51 (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 3.4e-10
Identity = 24/49 (48.98%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 188 DCSVCLTQFAPDSEINHLSCGHLFHRVCLEKWLDYWNLTCPLCRTPFMP 237
           DC VCL++     E+  L C H+FH+ CLE WL + N  CPLCR+P +P
Sbjct: 73  DCIVCLSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of Carg16124 vs. ExPASy Swiss-Prot
Match: Q9ZT50 (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-09
Identity = 27/68 (39.71%), Postives = 37/68 (54.41%), Query Frame = 0


Query: 188 DCSVCLTQFAPDSEINHLSCGHLFHRVCLEKWLDYWNLTCPLCRTPFMPEE--------- 245
           DC VCL++     E+  L C H+FH+ CLE WL  +N TCPLCR+  + ++         
Sbjct: 85  DCVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQRSV 144

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BLAST of Carg16124 vs. ExPASy TrEMBL
Match: A0A6J1FXQ9 (probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita moschata OX=3662 GN=LOC111448681 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.1e-85
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 60

Query: 152 SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 211
           SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF
Sbjct: 61  SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 120

Query: 212 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW
Sbjct: 121 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 154

[Close]

BLAST of Carg16124 vs. ExPASy TrEMBL
Match: A0A6J1JEA7 (probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita maxima OX=3661 GN=LOC111483745 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.5e-85
Identity = 153/154 (99.35%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 60

Query: 152 SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 211
           SIEFHLNPNG+YIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF
Sbjct: 61  SIEFHLNPNGNYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHLF 120

Query: 212 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW
Sbjct: 121 HRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 154

[Close]

BLAST of Carg16124 vs. ExPASy TrEMBL
Match: A0A0A0KAU4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G113550 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.4e-80
Identity = 148/155 (95.48%), Postives = 150/155 (96.77%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHL-SSPTLPSSPDSMENAP 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHL SSPTLPSSPDSMENAP
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSPTLPSSPDSMENAP 60

Query: 152 ESIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGHL 211
           ESIEFHLNPNGSYIEEFRSRIPAILF+KV SCK LEHDCSVCLTQF P+SEINHLSCGHL
Sbjct: 61  ESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHL 120

Query: 212 FHRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           FHRVCLEKWLDYWNLTCPLCRTP MPEEDTASCFW
Sbjct: 121 FHRVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 155

[Close]

BLAST of Carg16124 vs. ExPASy TrEMBL
Match: A0A5D3DTM0 (Putative E3 ubiquitin-protein ligase XERICO OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2527G00040 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.3e-78
Identity = 146/156 (93.59%), Postives = 148/156 (94.87%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHL--SSPTLPSSPDSMENA 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHL  SS TLPSSPDSMENA
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSSATLPSSPDSMENA 60

Query: 152 PESIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGH 211
           PESIEFHLNPNGSYIEEFRSRIPAILF+KV SCK LEHDCSVCLTQF P+SEINHLSCGH
Sbjct: 61  PESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120

Query: 212 LFHRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           LFH VCLEKWLDYWNLTCPLCRTP MPEEDTASCFW
Sbjct: 121 LFHSVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 156

[Close]

BLAST of Carg16124 vs. ExPASy TrEMBL
Match: A0A1S3BIY9 (probable E3 ubiquitin-protein ligase XERICO OS=Cucumis melo OX=3656 GN=LOC103490418 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.3e-78
Identity = 146/156 (93.59%), Postives = 148/156 (94.87%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHL--SSPTLPSSPDSMENA 151
           MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHL  SS TLPSSPDSMENA
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSSATLPSSPDSMENA 60

Query: 152 PESIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHLSCGH 211
           PESIEFHLNPNGSYIEEFRSRIPAILF+KV SCK LEHDCSVCLTQF P+SEINHLSCGH
Sbjct: 61  PESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120

Query: 212 LFHRVCLEKWLDYWNLTCPLCRTPFMPEEDTASCFW 246
           LFH VCLEKWLDYWNLTCPLCRTP MPEEDTASCFW
Sbjct: 121 LFHSVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 156

[Close]

BLAST of Carg16124 vs. TAIR 10
Match: AT2G04240.1 (RING/U-box superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 4.8e-44
Identity = 95/158 (60.13%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLS-SPTLPSSPD-SMENA 151
           MGLSSLP PSEG+L VILVNTALSISI KGIVRS L +VGI LS S + PSS   S EN+
Sbjct: 1   MGLSSLPGPSEGMLCVILVNTALSISIVKGIVRSFLGIVGISLSPSSSSPSSVTVSSENS 60

Query: 152 --PESIEFHLNPNGSYIEEFRSRIPAILFNKVHSC-KLLEHDCSVCLTQFAPDSEINHLS 211
              ES +F +    SY+EEFR+R P + F  +  C K  +++CSVCL++F  DSEIN L 
Sbjct: 61  STSESFDFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLK 120

Query: 212 CGHLFHRVCLEKWLDYWNLTCPLCRTPF--MPEEDTAS 243
           CGHLFH+ CLEKW+DYWN+TCPLCRTP   +PE+   S
Sbjct: 121 CGHLFHKTCLEKWIDYWNITCPLCRTPLVVVPEDHQLS 158

[Close]

BLAST of Carg16124 vs. TAIR 10
Match: AT2G04240.2 (RING/U-box superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 4.8e-44
Identity = 95/158 (60.13%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLS-SPTLPSSPD-SMENA 151
           MGLSSLP PSEG+L VILVNTALSISI KGIVRS L +VGI LS S + PSS   S EN+
Sbjct: 1   MGLSSLPGPSEGMLCVILVNTALSISIVKGIVRSFLGIVGISLSPSSSSPSSVTVSSENS 60

Query: 152 --PESIEFHLNPNGSYIEEFRSRIPAILFNKVHSC-KLLEHDCSVCLTQFAPDSEINHLS 211
              ES +F +    SY+EEFR+R P + F  +  C K  +++CSVCL++F  DSEIN L 
Sbjct: 61  STSESFDFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLK 120

Query: 212 CGHLFHRVCLEKWLDYWNLTCPLCRTPF--MPEEDTAS 243
           CGHLFH+ CLEKW+DYWN+TCPLCRTP   +PE+   S
Sbjct: 121 CGHLFHKTCLEKWIDYWNITCPLCRTPLVVVPEDHQLS 158

[Close]

BLAST of Carg16124 vs. TAIR 10
Match: AT1G67856.1 (RING/U-box superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.0e-14
Identity = 46/142 (32.39%), Postives = 72/142 (50.70%), Query Frame = 0


Query: 92  MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPE 151
           MG+S  P  SEGV+ ++++NT +S+S+ K +VRS++++V    +           E++  
Sbjct: 1   MGISHYPTASEGVMPMLVMNTVVSVSLVKNMVRSVVNMVSSETNEARNKEDDQDHEDSKR 60

Query: 152 SIEFHLNPNGSYIEEFRSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHL-SCGHL 211
                +    S  E   SR      N+  +      DC VCL  F  + E++ L SC H 
Sbjct: 61  RRRISITHFESLCENRGSR------NEREAM-----DCCVCLCGFKEEEEVSELVSCKHY 120

Query: 212 FHRVCLEKWLDYWNLTCPLCRT 233
           FH  CL+KW    + TCPLCR+
Sbjct: 121 FHSACLDKWFGNNHTTCPLCRS 131

[Close]

BLAST of Carg16124 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-14
Identity = 48/140 (34.29%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 101 SEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENAPESIEFHLNPN 160
           +E  L  + V T   +   + IV SI   +G+        + PD   + P  I    +P 
Sbjct: 8   TEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPD-YTSYPTRIPETRSPF 67

Query: 161 GSYIEEFRSRIPAILFNKV-HSCKLLEHDCSVCLTQFAPDSEINHL-SCGHLFHRVCLEK 220
            + +   R  +P I F ++ +S + L  +C+VCL +F  + EI  L +C H+FHR CL++
Sbjct: 68  SALL--IREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDR 127

Query: 221 WLDYWNLTCPLCRTPFMPEE 239
           W+D+   TCPLCRTPF+P+E
Sbjct: 128 WMDHDQKTCPLCRTPFVPDE 144

[Close]

BLAST of Carg16124 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-11
Identity = 43/140 (30.71%), Postives = 70/140 (50.00%), Query Frame = 0


Query: 109 LVNTALSISIFKGIVRSILHVVGIHLSSPTLPSSPDSMENA-PESIEFHLNPNGSYIEEF 168
           ++ T   I  F+ +V ++   +G       LPS  D  E +  +     L+ + +   E 
Sbjct: 16  VLKTLYVIGFFRDMVDALCPYIG-------LPSFLDHNETSRSDPTRLALSTSATLANEL 75

Query: 169 RSRIPAILFNKVHSCKLLEHDCSVCLTQFAPDSEINHL-SCGHLFHRVCLEKWL-DYWNL 228
              IP + F+ + +    E  C+VCL+ F  D +I  L  CGH+FH  CL++W+ D   +
Sbjct: 76  ---IPVVRFSDLLTDP--EDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDCNKI 135

Query: 229 TCPLCRTPFMPEEDTASCFW 246
           TCP+CR  F+PEE +    W
Sbjct: 136 TCPICRNRFLPEEKSTPFDW 143

The following BLAST results are available for this feature:

BLAST of Carg16124 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010803.1	6.5e-136	100.00	putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma sub...	[more]
KAG6570965.1	2.3e-85	100.00	putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma sub...	[more]
XP_022944133.1	2.3e-85	100.00	probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata] >XP_022944135.1...	[more]
XP_022985804.1	5.2e-85	99.35	probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima] >XP_022985805.1 p...	[more]
XP_023512749.1	5.8e-84	98.70	probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo] >XP_023...	[more]

BLAST of Carg16124 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SI09	6.7e-43	60.13	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=X...	[more]
Q9XF92	3.2e-13	34.29	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS5	2.0e-10	30.71	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q9ZU51	3.4e-10	48.98	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 ...	[more]
Q9ZT50	1.7e-09	39.71	E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 ...	[more]

BLAST of Carg16124 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FXQ9	1.1e-85	100.00	probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JEA7	2.5e-85	99.35	probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0KAU4	2.4e-80	95.48	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G113550 P...	[more]
A0A5D3DTM0	1.3e-78	93.59	Putative E3 ubiquitin-protein ligase XERICO OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BIY9	1.3e-78	93.59	probable E3 ubiquitin-protein ligase XERICO OS=Cucumis melo OX=3656 GN=LOC103490...	[more]

BLAST of Carg16124 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04240.1	4.8e-44	60.13	RING/U-box superfamily protein	[more]
AT2G04240.2	4.8e-44	60.13	RING/U-box superfamily protein	[more]
AT1G67856.1	1.0e-14	32.39	RING/U-box superfamily protein	[more]
AT3G61460.1	2.3e-14	34.29	brassinosteroid-responsive RING-H2	[more]
AT4G11360.1	1.4e-11	30.71	RING-H2 finger A1B	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 189..230 e-value: 4.5E-6 score: 36.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 188..231 e-value: 3.8E-10 score: 39.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 189..231 score: 12.636808
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 156..236 e-value: 6.1E-16 score: 59.9
None	No IPR available	PANTHER	PTHR47258:SF1	E3 UBIQUITIN-PROTEIN LIGASE XERICO-RELATED	coord: 92..245
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 180..237
IPR044249	Probable E3 ubiquitin-protein ligase XERICO-like	PANTHER	PTHR47258	FAMILY NOT NAMED	coord: 92..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16124-RA	Carg16124-RA	mRNA