Carg16093 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16093
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Thymidine kinase
Location	Carg_Chr20: 3221020 .. 3222150 (+)
RNA-Seq Expression	Carg16093
Synteny	Carg16093

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATCTCAGTCTCCTTCAAAATTGCGCCTCCGATTTCGATTCAAATTCTGTCCTATTTACTTTTTGTCTCCAATCTGCTCCATGGAGTCCTTGAGGAATTCATTTTCTTTGACCTCCACCGATGGTGGCGCCGTCAGTGCAGGATCTCCACACCGCGTCGCAGGCGAAGTCCATGTGATCATCGGTCCTATGTTTGCCGGAAAAACCACCGCTCTACTCCGTCGGATCAAAGCTGAGAGCAGCAGTGGCAGGTGAAGATAAAGTCTATTCTTACGGCTTGCAAACAGATCGAAAACCTGCATTAAAATCTCAACTTGGAATTTCTTTGACTCTCGTATCTGCTCTGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACAAGGTACGCTATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGACAGAAATTTGGAGAAGATGCTTATAACAAGGTATTCCTCTTTGAAGAACATTCTTTGGCATTACTATTTGTTTTAGTTATCTAAACCCCCAATAATACCGGCAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTCGATGATCTCTATGATTTTTGCTGCGATGTTGCTGATAAAGATGACAAAATCATTATCGTTGCTGGCTTAGATGGAGATTACCTCAGGTCCCACTCAGATCTCTCTCTGTCTTTCTCTGTATATATGACAATTCTGCATTCACATAATTTCTTGCATCTGAGCTGTTGGGTAACTTGAAAATTGTGCCACCCAGATGCGAAATCATGGATCTTTCCAATACTAAAGTGGTTATTCTTCCATGGTTTTGGCAGGAGGAGATTTGGTTCAGTGCTTGATGTAGTTCCTCTGGCTGACACAGTAACTAAGTTGACAGCTAGATGTGAACTTTGTGGTAAAAGAGCCTTCTTTACCTTGAGAAAGACAGAGGAGACAAGGACCGAGCTTATCGCTGGGGCTGATGTCTATATGCCGGTGTGCCGCCACCACTATGCCAATGGACAAGAAGTCATACAATCTTCCAAGAATATTCTGGGATCTCGTGCTCTTAGAACAGGCTCTAATCTAGAGGCACTTCCTTCACTTATCTAA

mRNA sequence

GATCTCAGTCTCCTTCAAAATTGCGCCTCCGATTTCGATTCAAATTCTGTCCTATTTACTTTTTGTCTCCAATCTGCTCCATGGAGTCCTTGAGGAATTCATTTTCTTTGACCTCCACCGATGGTGGCGCCGTCAGTGCAGGATCTCCACACCGCGTCGCAGGCGAAGTCCATGTGATCATCGGTCCTATGTTTGCCGGAAAAACCACCGCTCTACTCCGTCGGATCAAAGCTGAGAGCAGCAGTGGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACAAGGTACGCTATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGACAGAAATTTGGAGAAGATGCTTATAACAAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTCGATGATCTCTATGATTTTTGCTGCGATGTTGCTGATAAAGATGACAAAATCATTATCGTTGCTGGCTTAGATGGAGATTACCTCAGGAGGAGATTTGGTTCAGTGCTTGATGTAGTTCCTCTGGCTGACACAGTAACTAAGTTGACAGCTAGATGTGAACTTTGTGGTAAAAGAGCCTTCTTTACCTTGAGAAAGACAGAGGAGACAAGGACCGAGCTTATCGCTGGGGCTGATGTCTATATGCCGGTGTGCCGCCACCACTATGCCAATGGACAAGAAGTCATACAATCTTCCAAGAATATTCTGGGATCTCGTGCTCTTAGAACAGGCTCTAATCTAGAGGCACTTCCTTCACTTATCTAA

Coding sequence (CDS)

ATGGAGTCCTTGAGGAATTCATTTTCTTTGACCTCCACCGATGGTGGCGCCGTCAGTGCAGGATCTCCACACCGCGTCGCAGGCGAAGTCCATGTGATCATCGGTCCTATGTTTGCCGGAAAAACCACCGCTCTACTCCGTCGGATCAAAGCTGAGAGCAGCAGTGGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACAAGGTACGCTATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGACAGAAATTTGGAGAAGATGCTTATAACAAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTCGATGATCTCTATGATTTTTGCTGCGATGTTGCTGATAAAGATGACAAAATCATTATCGTTGCTGGCTTAGATGGAGATTACCTCAGGAGGAGATTTGGTTCAGTGCTTGATGTAGTTCCTCTGGCTGACACAGTAACTAAGTTGACAGCTAGATGTGAACTTTGTGGTAAAAGAGCCTTCTTTACCTTGAGAAAGACAGAGGAGACAAGGACCGAGCTTATCGCTGGGGCTGATGTCTATATGCCGGTGTGCCGCCACCACTATGCCAATGGACAAGAAGTCATACAATCTTCCAAGAATATTCTGGGATCTCGTGCTCTTAGAACAGGCTCTAATCTAGAGGCACTTCCTTCACTTATCTAA

Protein sequence

MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI

Homology

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BLAST of Carg16093 vs. NCBI nr
Match: KAG7010772.1 (Thymidine kinase a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 473.4 bits (1217), Expect = 1.1e-129
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 237

[Close]

BLAST of Carg16093 vs. NCBI nr
Match: XP_023513380.1 (thymidine kinase a [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.7 bits (1210), Expect = 7.4e-129
Identity = 236/237 (99.58%), Postives = 236/237 (99.58%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 237

[Close]

BLAST of Carg16093 vs. NCBI nr
Match: XP_022943895.1 (thymidine kinase a [Cucurbita moschata])

HSP 1 Score: 468.8 bits (1205), Expect = 2.8e-128
Identity = 235/237 (99.16%), Postives = 235/237 (99.16%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSFSL STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSFSLISTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDSKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 237

[Close]

BLAST of Carg16093 vs. NCBI nr
Match: KAG6570931.1 (Thymidine kinase a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 465.3 bits (1196), Expect = 3.1e-127
Identity = 233/237 (98.31%), Postives = 233/237 (98.31%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSFS TSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSFSFTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCW LPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWTLPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRT SNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTSSNLEALPSLI 237

[Close]

BLAST of Carg16093 vs. NCBI nr
Match: XP_022985744.1 (thymidine kinase-like [Cucurbita maxima])

HSP 1 Score: 459.9 bits (1182), Expect = 1.3e-125
Identity = 232/237 (97.89%), Postives = 233/237 (98.31%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSF   STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSF---STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA+DSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAVDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 234

[Close]

BLAST of Carg16093 vs. ExPASy Swiss-Prot
Match: O81263 (Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2)

HSP 1 Score: 309.3 bits (791), Expect = 3.8e-83
Identity = 143/209 (68.42%), Postives = 175/209 (83.73%), Query Frame = 0


Query: 12  STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSG-RNVVMIKSSKDTRY 71
           S  GG  +  +     GE+HVI+GPMFAGKTTALLRR++ E+ +G RNV +IKS KD RY
Sbjct: 51  SRGGGGSAMEAQPSYPGEIHVIVGPMFAGKTTALLRRVQVEAGTGSRNVALIKSDKDNRY 110

Query: 72  AIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADK 131
            +DSVVTHDG K PCWALP+LSSF+ K G +AY+K+DVIG+DEAQFFDDL+DFCC  AD+
Sbjct: 111 GLDSVVTHDGTKMPCWALPELSSFQDKLGTEAYDKVDVIGIDEAQFFDDLHDFCCKAADR 170

Query: 132 DDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTEL 191
           D KI++VAGLDGDY R +FGSVLD++PLAD+VTKLTARCELCG+RAFFTLRKT ET+TEL
Sbjct: 171 DGKIVVVAGLDGDYKRNKFGSVLDIIPLADSVTKLTARCELCGRRAFFTLRKTRETKTEL 230

Query: 192 IAGADVYMPVCRHHYANGQEVIQSSKNIL 220
           I GADVYMPVCR HY +GQ VI++++ +L
Sbjct: 231 IGGADVYMPVCRQHYLDGQIVIEATRIVL 259

[Close]

BLAST of Carg16093 vs. ExPASy Swiss-Prot
Match: Q9S750 (Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 4.9e-83
Identity = 151/222 (68.02%), Postives = 179/222 (80.63%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDG---GAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGR 60
           M +L+ SF + + D    G   +    R +G VHVI+GPMF+GK+T+LLRRIK+E S GR
Sbjct: 1   MATLKASFLIKTLDSDVTGDFLSDLERRGSGAVHVIMGPMFSGKSTSLLRRIKSEISDGR 60

Query: 61  NVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFF 120
           +V M+KSSKDTRYA DSVVTHDG  FPCWALPDL SF +KFG DAYNKLDVIG+DEAQFF
Sbjct: 61  SVAMLKSSKDTRYAKDSVVTHDGIGFPCWALPDLMSFPEKFGLDAYNKLDVIGIDEAQFF 120

Query: 121 DDLYDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAF 180
            DLY+FCC VAD D KI+IVAGLDGDYLRR FG+VLD++P+AD+VTKLTARCE+CG +AF
Sbjct: 121 GDLYEFCCKVADDDGKIVIVAGLDGDYLRRSFGAVLDIIPIADSVTKLTARCEVCGHKAF 180

Query: 181 FTLRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNIL 220
           FTLRK  +TRTELI GADVYMPVCR HY     VI++SK +L
Sbjct: 181 FTLRKNCDTRTELIGGADVYMPVCRKHYITNHIVIKASKKVL 222

[Close]

BLAST of Carg16093 vs. ExPASy Swiss-Prot
Match: F4KBF5 (Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-78
Identity = 135/212 (63.68%), Postives = 176/212 (83.02%), Query Frame = 0


Query: 11  TSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRY 70
           +S+    +S+ SP    GE+HV++GPMF+GKTT LLRRI AE  +G+ + +IKS+KDTRY
Sbjct: 64  SSSSSQPLSSSSP----GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDTRY 123

Query: 71  AIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY-NKLDVIGVDEAQFFDDLYDFCCDVAD 130
             +S+VTHDG K+PCW+LPDLSSF+++FG D Y N+LDVIG+DEAQFF DLY+FC + AD
Sbjct: 124 CTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAAD 183

Query: 131 KDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTE 190
           K+ K +IVAGLDGD++RRRFGSVLD++P+ADTVTKLT+RCE+CGKRA FT+RKTEE  TE
Sbjct: 184 KEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETE 243

Query: 191 LIAGADVYMPVCRHHYANGQEVIQSSKNILGS 222
           LI GA+VYMPVCR HY  GQ V+++++ +L S
Sbjct: 244 LIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

[Close]

BLAST of Carg16093 vs. ExPASy Swiss-Prot
Match: P23335 (Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.2e-34
Identity = 74/178 (41.57%), Postives = 112/178 (62.92%), Query Frame = 0


Query: 28  GEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRYAIDSVVTHDGAKFPCWA 87
           G +H+I+GPMF+GK+T L+R +     +  N  +IK SKD RY  D+V THD       +
Sbjct: 4   GFIHLILGPMFSGKSTELIRLVNRYQIATYNCRVIKYSKDNRYGNDAVYTHDKCYISAVS 63

Query: 88  LPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDKIIIVAGLDGDYLRR 147
              L   +     D  + +D++G+DE QFF+D+ +FC  +A+K  KI+IVA LDG Y R+
Sbjct: 64  TDSLFDIK-----DTLDDVDIVGIDEGQFFNDIVEFCEYIANK-GKIVIVAALDGTYERK 123

Query: 148 RFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRHHY 206
            FG++L+++PL++ VTKL A C +C + A F+ R ++E   ELI G + Y+ VCR  Y
Sbjct: 124 PFGNILNLIPLSEKVTKLNAICMICHRDASFSKRLSDEKEIELIGGKEKYLSVCRSCY 175

[Close]

BLAST of Carg16093 vs. ExPASy Swiss-Prot
Match: Q27564 (Thymidine kinase 1 OS=Dictyostelium discoideum OX=44689 GN=thyB PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 9.4e-34
Identity = 87/199 (43.72%), Postives = 119/199 (59.80%), Query Frame = 0


Query: 25  RVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRY--AIDS--VVTHDG 84
           ++AG++ VI GPMF+GKTT L+RRIK  + + +  ++IK SKDTRY   ID   +VTHD 
Sbjct: 5   QIAGKIQVIFGPMFSGKTTELIRRIKRFNFANKKCLLIKYSKDTRYNDNIDKSFLVTHDK 64

Query: 85  AK---FPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDLYDFCCDVADKDDKIIIV 144
                FPC  L D+    Q +        DVIG+DE QFF D+  F  D+A++  K +I+
Sbjct: 65  QNYQAFPCSILEDVKEQAQNY--------DVIGIDEGQFFPDVVQFSEDLANQ-GKTVII 124

Query: 145 AGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVY 204
           A LDG + R+ F SV+D+V  A+ +TKLTA C +C   A F+ R  E    ELI G D Y
Sbjct: 125 AALDGTFQRKPFQSVIDLVSKAEYITKLTAVCMVCYNEAAFSKRIVESDDIELIGGIDKY 184

Query: 205 MPVCRHHYANGQEVIQSSK 217
           + VCR  Y + Q    S+K
Sbjct: 185 ISVCRGCYNSDQNEGNSTK 194

[Close]

BLAST of Carg16093 vs. ExPASy TrEMBL
Match: A0A6J1FYC7 (Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111448484 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.4e-128
Identity = 235/237 (99.16%), Postives = 235/237 (99.16%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSFSL STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSFSLISTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDSKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 237

[Close]

BLAST of Carg16093 vs. ExPASy TrEMBL
Match: A0A6J1JE52 (Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111483705 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 6.4e-126
Identity = 232/237 (97.89%), Postives = 233/237 (98.31%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MESLRNSF   STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV
Sbjct: 1   MESLRNSF---STDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA+DSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAVDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCCDVADKD KIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI
Sbjct: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 234

[Close]

BLAST of Carg16093 vs. ExPASy TrEMBL
Match: A0A6J1CH59 (Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111011151 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.6e-116
Identity = 212/236 (89.83%), Postives = 224/236 (94.92%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           M+S R+S SLT+TDGG V AGS  RVAGEVHVIIGPMFAGKTTALLRRIK+ES+SGRNVV
Sbjct: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VADKD KI+IVAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSL 237
           RKTEETRTELIAGADVYMPVCR HYANGQ+VIQ+SKN+LGSR + TGSNLEAL SL
Sbjct: 181 RKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 236

[Close]

BLAST of Carg16093 vs. ExPASy TrEMBL
Match: A0A5D3CJB6 (Thymidine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G00640 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.5e-111
Identity = 203/237 (85.65%), Postives = 219/237 (92.41%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MES R+S SLTS++  A+   S HR+ GEVHVIIGPMFAGKTTALLRRIK+ S+SGRNVV
Sbjct: 1   MESCRSSLSLTSSNADAIRPESSHRLTGEVHVIIGPMFAGKTTALLRRIKSASNSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA+DSVVTHDGAKFPCWALP LSSFRQKFGEDAYN+LDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAVDSVVTHDGAKFPCWALPHLSSFRQKFGEDAYNELDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VAD+D KII+VAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCNVADRDGKIIVVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEET+TELIAGADVYMPVCRHHYA GQEVIQSSKN++GSR +   SNLEALPSLI
Sbjct: 181 RKTEETKTELIAGADVYMPVCRHHYATGQEVIQSSKNVMGSRTI--ASNLEALPSLI 235

[Close]

BLAST of Carg16093 vs. ExPASy TrEMBL
Match: A0A1S3CKW8 (Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103502126 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.5e-111
Identity = 203/237 (85.65%), Postives = 219/237 (92.41%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVV 60
           MES R+S SLTS++  A+   S HR+ GEVHVIIGPMFAGKTTALLRRIK+ S+SGRNVV
Sbjct: 1   MESCRSSLSLTSSNADAIRPESSHRLTGEVHVIIGPMFAGKTTALLRRIKSASNSGRNVV 60

Query: 61  MIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDDL 120
           MIKSSKDTRYA+DSVVTHDGAKFPCWALP LSSFRQKFGEDAYN+LDVIGVDEAQFFDDL
Sbjct: 61  MIKSSKDTRYAVDSVVTHDGAKFPCWALPHLSSFRQKFGEDAYNELDVIGVDEAQFFDDL 120

Query: 121 YDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180
           YDFCC+VAD+D KII+VAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFTL
Sbjct: 121 YDFCCNVADRDGKIIVVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTL 180

Query: 181 RKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSLI 238
           RKTEET+TELIAGADVYMPVCRHHYA GQEVIQSSKN++GSR +   SNLEALPSLI
Sbjct: 181 RKTEETKTELIAGADVYMPVCRHHYATGQEVIQSSKNVMGSRTI--ASNLEALPSLI 235

[Close]

BLAST of Carg16093 vs. TAIR 10
Match: AT3G07800.1 (Thymidine kinase )

HSP 1 Score: 308.9 bits (790), Expect = 3.5e-84
Identity = 151/222 (68.02%), Postives = 179/222 (80.63%), Query Frame = 0


Query: 1   MESLRNSFSLTSTDG---GAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGR 60
           M +L+ SF + + D    G   +    R +G VHVI+GPMF+GK+T+LLRRIK+E S GR
Sbjct: 1   MATLKASFLIKTLDSDVTGDFLSDLERRGSGAVHVIMGPMFSGKSTSLLRRIKSEISDGR 60

Query: 61  NVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFF 120
           +V M+KSSKDTRYA DSVVTHDG  FPCWALPDL SF +KFG DAYNKLDVIG+DEAQFF
Sbjct: 61  SVAMLKSSKDTRYAKDSVVTHDGIGFPCWALPDLMSFPEKFGLDAYNKLDVIGIDEAQFF 120

Query: 121 DDLYDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAF 180
            DLY+FCC VAD D KI+IVAGLDGDYLRR FG+VLD++P+AD+VTKLTARCE+CG +AF
Sbjct: 121 GDLYEFCCKVADDDGKIVIVAGLDGDYLRRSFGAVLDIIPIADSVTKLTARCEVCGHKAF 180

Query: 181 FTLRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNIL 220
           FTLRK  +TRTELI GADVYMPVCR HY     VI++SK +L
Sbjct: 181 FTLRKNCDTRTELIGGADVYMPVCRKHYITNHIVIKASKKVL 222

[Close]

BLAST of Carg16093 vs. TAIR 10
Match: AT5G23070.1 (Thymidine kinase )

HSP 1 Score: 293.9 bits (751), Expect = 1.2e-79
Identity = 135/212 (63.68%), Postives = 176/212 (83.02%), Query Frame = 0


Query: 11  TSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSGRNVVMIKSSKDTRY 70
           +S+    +S+ SP    GE+HV++GPMF+GKTT LLRRI AE  +G+ + +IKS+KDTRY
Sbjct: 64  SSSSSQPLSSSSP----GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDTRY 123

Query: 71  AIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY-NKLDVIGVDEAQFFDDLYDFCCDVAD 130
             +S+VTHDG K+PCW+LPDLSSF+++FG D Y N+LDVIG+DEAQFF DLY+FC + AD
Sbjct: 124 CTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAAD 183

Query: 131 KDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTE 190
           K+ K +IVAGLDGD++RRRFGSVLD++P+ADTVTKLT+RCE+CGKRA FT+RKTEE  TE
Sbjct: 184 KEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETE 243

Query: 191 LIAGADVYMPVCRHHYANGQEVIQSSKNILGS 222
           LI GA+VYMPVCR HY  GQ V+++++ +L S
Sbjct: 244 LIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

The following BLAST results are available for this feature:

BLAST of Carg16093 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010772.1	1.1e-129	100.00	Thymidine kinase a [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023513380.1	7.4e-129	99.58	thymidine kinase a [Cucurbita pepo subsp. pepo]	[more]
XP_022943895.1	2.8e-128	99.16	thymidine kinase a [Cucurbita moschata]	[more]
KAG6570931.1	3.1e-127	98.31	Thymidine kinase a, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022985744.1	1.3e-125	97.89	thymidine kinase-like [Cucurbita maxima]	[more]

BLAST of Carg16093 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81263	3.8e-83	68.42	Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2	[more]
Q9S750	4.9e-83	68.02	Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1	[more]
F4KBF5	1.6e-78	63.68	Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1	[more]
P23335	4.2e-34	41.57	Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1	[more]
Q27564	9.4e-34	43.72	Thymidine kinase 1 OS=Dictyostelium discoideum OX=44689 GN=thyB PE=1 SV=1	[more]

BLAST of Carg16093 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FYC7	1.4e-128	99.16	Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111448484 PE=3 SV=1	[more]
A0A6J1JE52	6.4e-126	97.89	Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111483705 PE=3 SV=1	[more]
A0A6J1CH59	4.6e-116	89.83	Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111011151 PE=3 SV=1	[more]
A0A5D3CJB6	7.5e-111	85.65	Thymidine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G006...	[more]
A0A1S3CKW8	7.5e-111	85.65	Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103502126 PE=3 SV=1	[more]

BLAST of Carg16093 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07800.1	3.5e-84	68.02	Thymidine kinase	[more]
AT5G23070.1	1.2e-79	63.68	Thymidine kinase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001267	Thymidine kinase	PFAM	PF00265	TK	coord: 28..205 e-value: 1.1E-52 score: 178.5
IPR001267	Thymidine kinase	PANTHER	PTHR11441	THYMIDINE KINASE	coord: 18..226
None	No IPR available	GENE3D	3.30.60.20		coord: 166..207 e-value: 1.3E-15 score: 59.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..18
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR11441:SF8	THYMIDINE KINASE B	coord: 18..226
None	No IPR available	SUPERFAMILY	57716	Glucocorticoid receptor-like (DNA-binding domain)	coord: 166..208
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 23..165 e-value: 2.7E-45 score: 155.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 28..166
IPR020633	Thymidine kinase, conserved site	PROSITE	PS00603	TK_CELLULAR_TYPE	coord: 192..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16093-RA	Carg16093-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009157	deoxyribonucleoside monophosphate biosynthetic process
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0046104	thymidine metabolic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004797	thymidine kinase activity