Carg16069 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg16069 vs. NCBI nr
Match: KAG7026770.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.4 bits (2284), Expect = 4.1e-253
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60
           MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI
Sbjct: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60

Query: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120
           ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI
Sbjct: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120

Query: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180
           TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV
Sbjct: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180

Query: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240
           QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK
Sbjct: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240

Query: 241 LLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 300
           LLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG
Sbjct: 241 LLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 300

Query: 301 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 360
           EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE
Sbjct: 301 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 360

Query: 361 TLRMANIINGVWRKAQKDGIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTY 420
           TLRMANIINGVWRKAQKDGIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTY
Sbjct: 361 TLRMANIINGVWRKAQKDGIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTY 420

Query: 421 KWGAERDEIIHFPTVRMRRKLPIAIAKH 449
           KWGAERDEIIHFPTVRMRRKLPIAIAKH
Sbjct: 421 KWGAERDEIIHFPTVRMRRKLPIAIAKH 448

BLAST of Carg16069 vs. NCBI nr
Match: KAG6594802.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 858.2 bits (2216), Expect = 3.1e-245
Identity = 447/489 (91.41%), Postives = 447/489 (91.41%), Query Frame = 0

Query: 1   MEWVLGFWVWFLSVVALACLLFK-NNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAF 60
           MEWVLGFWVWFLSVVALACLLFK NNNNNT NNNKIHIQNKPGIPKGSLGWPFLGETLAF
Sbjct: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNNTNNNNKIHIQNKPGIPKGSLGWPFLGETLAF 60

Query: 61  IASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKS 120
           IASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKS
Sbjct: 61  IASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKS 120

Query: 121 ITELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVY 180
           ITELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVY
Sbjct: 121 ITELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVY 180

Query: 181 VQTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKARE 240
           VQTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKARE
Sbjct: 181 VQTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKARE 240

Query: 241 KLLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIP 300
           KLLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIP
Sbjct: 241 KLLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIP 300

Query: 301 GEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVIS 360
           GEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVIS
Sbjct: 301 GEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVIS 360

Query: 361 ETLRMANIINGVWRKAQKD----------------------------------------G 420
           ETLRMANIINGVWRKAQKD                                        G
Sbjct: 361 ETLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDENNYTNSHQFDPWRWEG 420

Query: 421 IGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRR 449
           IGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRR
Sbjct: 421 IGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRR 480

BLAST of Carg16069 vs. NCBI nr
Match: XP_022963240.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita moschata])

HSP 1 Score: 847.0 bits (2187), Expect = 7.2e-242
Identity = 444/491 (90.43%), Postives = 445/491 (90.63%), Query Frame = 0

Query: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60
           MEWVLGFWVWFLSVVALACLLFKNNNNNT   NKIHIQNKPGIPKGSLGWPFLGETLAFI
Sbjct: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNT---NKIHIQNKPGIPKGSLGWPFLGETLAFI 60

Query: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120
           ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI
Sbjct: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120

Query: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180
           TELLGSFSILQMNGNFQKRIHS+IGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV
Sbjct: 121 TELLGSFSILQMNGNFQKRIHSLIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180

Query: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240
           QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK
Sbjct: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240

Query: 241 LLKIVSKIVEERRSTAAAAAA---EEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELM 300
           LLKIVSKIVEERRSTAAAAAA   EEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELM
Sbjct: 241 LLKIVSKIVEERRSTAAAAAAAEEEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELM 300

Query: 301 IPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNV 360
           IPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNV
Sbjct: 301 IPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNV 360

Query: 361 ISETLRMANIINGVWRKAQKD--------------------------------------- 420
           ISETLRMANIINGVWRKAQKD                                       
Sbjct: 361 ISETLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDENNYTNSHQFDPWRW 420

Query: 421 -GIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRM 449
            GIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRM
Sbjct: 421 EGIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRM 480

BLAST of Carg16069 vs. NCBI nr
Match: XP_023518560.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842.4 bits (2175), Expect = 1.8e-240
Identity = 441/489 (90.18%), Postives = 443/489 (90.59%), Query Frame = 0

Query: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60
           MEWVLGFWVWFLSVVALACLLFKNNNN   NN+KIHIQNKPGIPKGSLGWPFLGETLAFI
Sbjct: 1   MEWVLGFWVWFLSVVALACLLFKNNNN---NNDKIHIQNKPGIPKGSLGWPFLGETLAFI 60

Query: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120
           ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI
Sbjct: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120

Query: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180
           TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV
Sbjct: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180

Query: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240
           QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK
Sbjct: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240

Query: 241 LLKIVSKIVEERRSTAAAA-AAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIP 300
           LLKIVSKIVEERRSTAAAA   EEK+PPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIP
Sbjct: 241 LLKIVSKIVEERRSTAAAAEEEEEKVPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIP 300

Query: 301 GEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVIS 360
           GEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVIS
Sbjct: 301 GEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVIS 360

Query: 361 ETLRMANIINGVWRKAQKD----------------------------------------G 420
           ETLRMANIINGVWRKAQKD                                        G
Sbjct: 361 ETLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDENNYTNSHQFDPWRWEG 420

Query: 421 IGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRR 449
           IGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRR
Sbjct: 421 IGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRR 480

BLAST of Carg16069 vs. NCBI nr
Match: XP_023002922.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita maxima])

HSP 1 Score: 828.2 bits (2138), Expect = 3.5e-236
Identity = 434/488 (88.93%), Postives = 437/488 (89.55%), Query Frame = 0

Query: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60
           MEWVLGFWVWFLSVVALACLLFKN+++N  NNNKIHIQNKPGIPKGSLGWPFLGETLAFI
Sbjct: 1   MEWVLGFWVWFLSVVALACLLFKNHHHN-NNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60

Query: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120
           ASGYSSRPVTFMN RKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI
Sbjct: 61  ASGYSSRPVTFMNNRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120

Query: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180
           TELLGSFSILQMNGNFQKRIHSIIGAFLKSP LKARITTDIQNTVKLRLSNWNTSKLVYV
Sbjct: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPGLKARITTDIQNTVKLRLSNWNTSKLVYV 180

Query: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240
           QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK
Sbjct: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240

Query: 241 LLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 300
           LLKIVSKIVEERRST   AA EEK  PRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG
Sbjct: 241 LLKIVSKIVEERRST---AAEEEKASPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 300

Query: 301 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 360
           EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE
Sbjct: 301 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 360

Query: 361 TLRMANIINGVWRKAQKD----------------------------------------GI 420
           TLRMANIINGVWRKAQKD                                        GI
Sbjct: 361 TLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDENNYTNAHQFDPWRWEGI 420

Query: 421 GSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK 449
           GSGS NQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK
Sbjct: 421 GSGSKNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK 480

BLAST of Carg16069 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 540.4 bits (1391), Expect = 1.9e-152
Identity = 289/483 (59.83%), Postives = 348/483 (72.05%), Query Frame = 0

Query: 8   WVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPG-IPKGSLGWPFLGETLAFIASGYSS 67
           W+W         L  +N+     +  + + + K G IP GSLGWP +GETL FIA GYSS
Sbjct: 42  WLW---------LRLRNSKTKDGDEEEDNEEKKKGMIPNGSLGWPVIGETLNFIACGYSS 101

Query: 68  RPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITELLGS 127
           RPVTFM+KRKSLYG VFKTNI+GTPII+STD++VNKVVLQNHGNTFVPAYPKSITELLG 
Sbjct: 102 RPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKVVLQNHGNTFVPAYPKSITELLGE 161

Query: 128 FSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYVQTETKQ 187
            SIL +NG  QKR+H++IGAFL+SP LK RIT DI+ +V L L++W    LV+VQ E K+
Sbjct: 162 NSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIEASVVLTLASWAQLPLVHVQDEIKK 221

Query: 188 ITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLKIVS 247
           +TFE+LVKVLMS  PGED+ ILK EFEEFIK LICIP+K PGTRLYKSLKA+E+L+K+V 
Sbjct: 222 MTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICIPIKFPGTRLYKSLKAKERLIKMVK 281

Query: 248 KIVEERRSTAAAAAAEEKIPPRDAVDVLLQD--NDVVQGLPLDFISSHIIELMIPGEETV 307
           K+VEER+      A     P  D VDVLL+D  +   Q  P DF+S  I+E+MIPGEET+
Sbjct: 282 KVVEERQ-----VAMTTTSPANDVVDVLLRDGGDSEKQSQPSDFVSGKIVEMMIPGEETM 341

Query: 308 PMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISETLRM 367
           P AMTLAVKFLSD PVALA+++EENME+K++K + GEEY WTDYMSL FTQNVI+ETLRM
Sbjct: 342 PTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRM 401

Query: 368 ANIINGVWRKAQKDG-----------------------------------------IGSG 427
           ANIINGVWRKA KD                                           GS 
Sbjct: 402 ANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSA 461

Query: 428 SNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRKLPI 447
           +++  FTPFGGGQRLCPG+EL++LEISIFLHHLVT Y W AE DEI+ FPTV+M+R+LPI
Sbjct: 462 NSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTAEEDEIVSFPTVKMKRRLPI 510

BLAST of Carg16069 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 3.3e-112
Identity = 205/442 (46.38%), Postives = 305/442 (69.00%), Query Frame = 0

Query: 43  IPKGSLGWPFLGETLAFIASGYSSRPVTFMNKRKSLYGS-VFKTNILGTPIIVSTDSDVN 102
           +P GS GWP +GETL F++  YS RP  F++KR+ L+GS VF++++ G+  +V+ D++V+
Sbjct: 49  LPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEVS 108

Query: 103 KVVLQNHGNTFVPAYPKSITELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDI 162
           + VLQ+    FVP YP+S+TEL+G  SIL +NG  Q+R+H ++GAF KS  LK+++T D+
Sbjct: 109 RFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTADM 168

Query: 163 QNTVKLRLSNWNTSKLVYVQTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALIC 222
           +  +   LS++  S L++VQ   K + FE+LV+ L+ +  GE+++ LK++F+EFI  L+ 
Sbjct: 169 RRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLMS 228

Query: 223 IPVKLPGTRLYKSLKAREKLLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVV 282
           +P+KLPGTRLY+SL+A++K+ +++ +I+ E+R+  AAA+     PPRDA+DVL+ D    
Sbjct: 229 LPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAAS-----PPRDAIDVLIGDGS-- 288

Query: 283 QGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGE 342
             L  + IS ++I+LMIP E++VP+ +TLAVKFLS+CP+AL Q+ EEN++LK++K D GE
Sbjct: 289 DELTDELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGE 348

Query: 343 EYTWTDYMSLLFTQNVISETLRMANIINGVWRKAQKDGIGSG------------------ 402
              WTDYMSL FTQ+VI+ETLR+ NII G+ RKA +D    G                  
Sbjct: 349 TLQWTDYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHL 408

Query: 403 --------------------SNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWG 446
                                +N +FTPFGGGQRLCPG++LARLE SIFLHHLVT+++W 
Sbjct: 409 DDTLYDEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWV 468

BLAST of Carg16069 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.9e-108
Identity = 212/464 (45.69%), Postives = 301/464 (64.87%), Query Frame = 0

Query: 23  KNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFIASGYSSRPVTFMNKRKSLYGSV 82
           K N+++ T+ +     + P  P GSLGWP +GET+ F++S YS RP +FM+KR+ +YG V
Sbjct: 36  KLNDHHVTSQS-----HGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRV 95

Query: 83  FKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITELLGSFSILQMNGNFQKRIHS 142
           FK++I GT  IVSTD++VN+ VLQ+    FVP YPK++ EL+G  SIL +NG+  +R H 
Sbjct: 96  FKSHIFGTATIVSTDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHG 155

Query: 143 IIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYVQTETKQITFEVLVKVLMSVGPG 202
           ++G+FLKSP LKA+I  D+   +   +  W+  + V +Q  +K + F+VL K L+SV  G
Sbjct: 156 LVGSFLKSPLLKAQIVRDMHKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKG 215

Query: 203 EDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLKIVSKIVEER-RSTAAAAAA 262
           EDL+ LKREFE FI  L+ +P+  PGT+L++SL+A++ ++K V +I+E + R T      
Sbjct: 216 EDLEELKREFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKT--KNKE 275

Query: 263 EEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLSDCPVA 322
           E+ +  +D VDVLL+D+   + L  + I++++I++MIPG ++VP+ +TLAVKFLSD P A
Sbjct: 276 EDDVIAKDVVDVLLKDSS--EHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAA 335

Query: 323 LAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISETLRMANIINGVWRKAQKDGIG 382
           L  + EENM+LK  K+ +GE   W DY+SL FTQ VI+ETLRM N+I GV RKA KD   
Sbjct: 336 LNLLTEENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEI 395

Query: 383 SG--------------------------------------SNNQNFTPFGGGQRLCPGIE 442
            G                                       N  +F+PFGGGQRLCPG++
Sbjct: 396 KGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLD 455

Query: 443 LARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRKLPIAIAK 448
           LARLE S+FLHHLVT ++W AE D II+FPTV M+ KLPI I +
Sbjct: 456 LARLETSVFLHHLVTRFRWIAEEDTIINFPTVHMKNKLPIWIKR 490

BLAST of Carg16069 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 3.2e-75
Identity = 170/449 (37.86%), Postives = 251/449 (55.90%), Query Frame = 0

Query: 42  GIPKGSLGWPFLGETLAFIASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVN 101
           G+P GSLG P +GET   I +  +  P  F+++R + YGSVF T++ G P I S D + N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 102 KVVLQNHGNTFVPAYPKSITELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDI 161
           + VLQN G  F  +YP SI  LLG  S+L M G+  KR+HS+  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 162 QNTVKLRLSNWNTSKLVYVQTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALIC 221
              V+  L +W  S  V +  E K+ITFE+ VK LMS  PGE  + L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 222 IPVKLPGTRLYKSLKAREKLLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVV 281
           +P+ L  T   K+++AR K+ + ++ +V +RR      A  +K    D +  LL  +D  
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKK----DMLAALLAADD-- 269

Query: 282 QGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGE 341
            G   + I   ++ L++ G ET    MTLAVKFL++ P+ALAQ+ EE+ +++  K DS  
Sbjct: 270 -GFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-Y 329

Query: 342 EYTWTDYMSLLFTQNVISETLRMANIINGVWRKA-------------------------- 401
              W+DY S+ FTQ V++ETLR+ANII GV+R+A                          
Sbjct: 330 SLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHL 389

Query: 402 ----------------QKDGIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTT 448
                           Q + + +G +N  FTPFGGG RLCPG ELAR+ +S+FLH LVT 
Sbjct: 390 DPNHFKDARTFNPWRWQSNSVTTGPSNV-FTPFGGGPRLCPGYELARVALSVFLHRLVTG 449

BLAST of Carg16069 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.2e-66
Identity = 162/483 (33.54%), Postives = 252/483 (52.17%), Query Frame = 0

Query: 9   VWFLSVVALACLLFK----NNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFIASGY 68
           V F + VAL  L+++          T+N K     +  +P GS GWP +GET ++  S  
Sbjct: 10  VVFTAAVALLHLIYRWWNAQRGQKRTSNEK---NQELHLPPGSTGWPLIGETYSYYRSMT 69

Query: 69  SSRPVTFMNKRKSLYGS-VFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITEL 128
           S+RP  F++ R+  Y S VF +++ G+  ++S+D   NK VLQN G  F   YPK++  L
Sbjct: 70  SNRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQFNKYVLQNEGRFFQAHYPKALKAL 129

Query: 129 LGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYVQTE 188
           +G + +L ++G+ Q+++H I    L+   LK     +IQN V   L  W   K + +Q E
Sbjct: 130 IGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEIQNLVHSTLDRWVDKKEIALQNE 189

Query: 189 TKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLK 248
             Q+   ++ K L+ + P ++   +   F ++  A+I IP+K+PG+   K LKARE L++
Sbjct: 190 CHQMVLNLMAKQLLDLSPSKETNEICELFVDYTNAVIAIPIKIPGSTYAKGLKARELLIR 249

Query: 249 IVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPGEET 308
            +S +++ERR           I  +D +  LL+++ +   +  DF    I+ L+  G ET
Sbjct: 250 KISNMIKERRD-------HPHIVHKDLLTKLLEEDSISDEIICDF----ILFLLFAGHET 309

Query: 309 VPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISETLR 368
              AMT A+KFL+ CP AL QM EE+  + K K    ++  W DY S+ FTQ VI+ETLR
Sbjct: 310 SSRAMTFAIKFLTTCPKALTQMKEEHDAILKAK-GGHKKLEWDDYKSMKFTQCVINETLR 369

Query: 369 MANIINGVWRKAQKDG------IGSG---------------------------------- 428
           + N   GV+R+ ++D       I  G                                  
Sbjct: 370 LGNFGPGVFRETKEDTKVKDCLIPKGWVVFAFLTATHLDEKFHNEALTFNPWRWELDQDV 429

Query: 429 SNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDE-IIHFPTVRMRRKLP 446
           SNN  F+PFGGG RLCPG  LARLE+++FLH  +T ++W A  DE   +FP   + +  P
Sbjct: 430 SNNHLFSPFGGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFP 477

BLAST of Carg16069 vs. ExPASy TrEMBL
Match: A0A6J1HHF1 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111463512 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.5e-242
Identity = 444/491 (90.43%), Postives = 445/491 (90.63%), Query Frame = 0

Query: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60
           MEWVLGFWVWFLSVVALACLLFKNNNNNT   NKIHIQNKPGIPKGSLGWPFLGETLAFI
Sbjct: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNT---NKIHIQNKPGIPKGSLGWPFLGETLAFI 60

Query: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120
           ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI
Sbjct: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120

Query: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180
           TELLGSFSILQMNGNFQKRIHS+IGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV
Sbjct: 121 TELLGSFSILQMNGNFQKRIHSLIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180

Query: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240
           QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK
Sbjct: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240

Query: 241 LLKIVSKIVEERRSTAAAAAA---EEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELM 300
           LLKIVSKIVEERRSTAAAAAA   EEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELM
Sbjct: 241 LLKIVSKIVEERRSTAAAAAAAEEEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELM 300

Query: 301 IPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNV 360
           IPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNV
Sbjct: 301 IPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNV 360

Query: 361 ISETLRMANIINGVWRKAQKD--------------------------------------- 420
           ISETLRMANIINGVWRKAQKD                                       
Sbjct: 361 ISETLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDENNYTNSHQFDPWRW 420

Query: 421 -GIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRM 449
            GIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRM
Sbjct: 421 EGIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRM 480

BLAST of Carg16069 vs. ExPASy TrEMBL
Match: A0A6J1KKY8 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111496682 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.7e-236
Identity = 434/488 (88.93%), Postives = 437/488 (89.55%), Query Frame = 0

Query: 1   MEWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60
           MEWVLGFWVWFLSVVALACLLFKN+++N  NNNKIHIQNKPGIPKGSLGWPFLGETLAFI
Sbjct: 1   MEWVLGFWVWFLSVVALACLLFKNHHHN-NNNNKIHIQNKPGIPKGSLGWPFLGETLAFI 60

Query: 61  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120
           ASGYSSRPVTFMN RKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI
Sbjct: 61  ASGYSSRPVTFMNNRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 120

Query: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 180
           TELLGSFSILQMNGNFQKRIHSIIGAFLKSP LKARITTDIQNTVKLRLSNWNTSKLVYV
Sbjct: 121 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPGLKARITTDIQNTVKLRLSNWNTSKLVYV 180

Query: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240
           QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK
Sbjct: 181 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 240

Query: 241 LLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 300
           LLKIVSKIVEERRST   AA EEK  PRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG
Sbjct: 241 LLKIVSKIVEERRST---AAEEEKASPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 300

Query: 301 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 360
           EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE
Sbjct: 301 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 360

Query: 361 TLRMANIINGVWRKAQKD----------------------------------------GI 420
           TLRMANIINGVWRKAQKD                                        GI
Sbjct: 361 TLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDENNYTNAHQFDPWRWEGI 420

Query: 421 GSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK 449
           GSGS NQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK
Sbjct: 421 GSGSKNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK 480

BLAST of Carg16069 vs. ExPASy TrEMBL
Match: A0A6J1BUA0 (3-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005423 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.6e-189
Identity = 360/485 (74.23%), Postives = 389/485 (80.21%), Query Frame = 0

Query: 2   EWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPG-IPKGSLGWPFLGETLAFI 61
           +WVLGFWV FLS V L    + N   N        I NK G IPKG+LGWPF+GETL FI
Sbjct: 4   DWVLGFWVCFLSAV-LGSWFWMNKMRN--------IDNKDGRIPKGNLGWPFIGETLQFI 63

Query: 62  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 121
           ASGYSSRPVTFM+KRKSLYG VFKTNILGTPIIVSTDS+VNKVVLQNHGN F+PAYPKSI
Sbjct: 64  ASGYSSRPVTFMDKRKSLYGEVFKTNILGTPIIVSTDSEVNKVVLQNHGNIFIPAYPKSI 123

Query: 122 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 181
           TELLG FSILQMNGN Q+++HSIIGAFL+SP LK+RIT DI NTV+L L+NWN S LVYV
Sbjct: 124 TELLGPFSILQMNGNVQRKVHSIIGAFLRSPLLKSRITKDIHNTVRLSLANWNPSHLVYV 183

Query: 182 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 241
           QT+TKQITFEVLVKVLMSVGPGEDLKILKREFEEFIK LICIP+KLPGTRLYKSLKAREK
Sbjct: 184 QTQTKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKGLICIPLKLPGTRLYKSLKAREK 243

Query: 242 LLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 301
           LLKIV KIVEE++     A AE+  P +DAV+VLL+D++  QGLPLDFISSH+IELMIPG
Sbjct: 244 LLKIVRKIVEEKK----WAMAEKAAPAKDAVEVLLEDHEGSQGLPLDFISSHVIELMIPG 303

Query: 302 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 361
           EETVPMAMTLAVKFLSD PVALAQ MEEN ELKKQKD SGEEYTWTD+MSL FTQNVISE
Sbjct: 304 EETVPMAMTLAVKFLSDSPVALAQTMEENKELKKQKDSSGEEYTWTDFMSLQFTQNVISE 363

Query: 362 TLRMANIINGVWRKAQKD----------------------------------------GI 421
           TLRMANIINGVWRKAQKD                                         I
Sbjct: 364 TLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDEENYANPHQFNPWRWEEI 423

Query: 422 GSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK 446
           GS +NN NFTPFGGGQRLCPGIELARLEISIFLH LVTTYKW AE+DEIIHFPTV+MRRK
Sbjct: 424 GSATNNHNFTPFGGGQRLCPGIELARLEISIFLHCLVTTYKWRAEKDEIIHFPTVKMRRK 475

BLAST of Carg16069 vs. ExPASy TrEMBL
Match: A0A1S3B2I5 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103485087 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.9e-184
Identity = 342/481 (71.10%), Postives = 382/481 (79.42%), Query Frame = 0

Query: 5   LGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFIASGY 64
           +GFWVWF+ V+ L   LFK          K  I+NK GIPKG+LGWPF GETL FI+SGY
Sbjct: 1   MGFWVWFVCVI-LGWFLFK--------KKKKIIENKDGIPKGNLGWPFFGETLQFISSGY 60

Query: 65  SSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITELL 124
           SSRPVTFM+KRKSLYG+VFKTNILGTPIIVSTDS+VNKV+LQNHGN F PAYPKSITELL
Sbjct: 61  SSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELL 120

Query: 125 GSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYVQTET 184
           G++SIL+M GN Q+R+H+IIGAFL+SP LKA+IT DIQNTV+LRL+NWN +  ++ QTE 
Sbjct: 121 GTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEA 180

Query: 185 KQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLKI 244
           KQITFEVLVKVLMSVGPG++L+ LKREFEEFIKALICIP+KLPGTRLYKSLKAREKLLKI
Sbjct: 181 KQITFEVLVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKI 240

Query: 245 VSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPGEETV 304
           V KIVEER+S        EK  PRDAVDVLLQD+   QGLPLDFISSHIIELMIPGEETV
Sbjct: 241 VGKIVEERKSRVG-----EKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETV 300

Query: 305 PMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISETLRM 364
           P AMTLAVKFL DCP ALAQ+MEENM+LKK+KD SGEEYTWTDYMSL FTQNVISETLRM
Sbjct: 301 PTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRM 360

Query: 365 ANIINGVWRKAQKD----------------------------------------GIGSGS 424
           ANI+NGVWRKAQKD                                           S +
Sbjct: 361 ANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEENLSAT 420

Query: 425 NNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRKLPIA 446
           NN NFTPFGGGQRLCPG+EL RLEISIFLHHLVTTY+W AE+DEI+HFPTV+MRRKLPI 
Sbjct: 421 NNHNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYRWEAEKDEIVHFPTVKMRRKLPIT 467

BLAST of Carg16069 vs. ExPASy TrEMBL
Match: A0A6J1BWQ0 (3-epi-6-deoxocathasterone 23-monooxygenase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005423 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.8e-183
Identity = 352/485 (72.58%), Postives = 381/485 (78.56%), Query Frame = 0

Query: 2   EWVLGFWVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPG-IPKGSLGWPFLGETLAFI 61
           +WVLGFWV FLS V L    + N   N        I NK G IPKG+LGWPF+GETL FI
Sbjct: 4   DWVLGFWVCFLSAV-LGSWFWMNKMRN--------IDNKDGRIPKGNLGWPFIGETLQFI 63

Query: 62  ASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSI 121
           ASGYSSRPVTFM+KRKSLYG VFKTNILGTPIIVSTDS+VNKVVLQNHGN F+PAYPKSI
Sbjct: 64  ASGYSSRPVTFMDKRKSLYGEVFKTNILGTPIIVSTDSEVNKVVLQNHGNIFIPAYPKSI 123

Query: 122 TELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYV 181
           TELLG FSILQMNGN Q+++HSIIGAFL+SP LK+RIT DI NTV+L L+NWN S LVYV
Sbjct: 124 TELLGPFSILQMNGNVQRKVHSIIGAFLRSPLLKSRITKDIHNTVRLSLANWNPSHLVYV 183

Query: 182 QTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREK 241
           QT+TKQ        VLMSVGPGEDLKILKREFEEFIK LICIP+KLPGTRLYKSLKAREK
Sbjct: 184 QTQTKQ--------VLMSVGPGEDLKILKREFEEFIKGLICIPLKLPGTRLYKSLKAREK 243

Query: 242 LLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPG 301
           LLKIV KIVEE++     A AE+  P +DAV+VLL+D++  QGLPLDFISSH+IELMIPG
Sbjct: 244 LLKIVRKIVEEKK----WAMAEKAAPAKDAVEVLLEDHEGSQGLPLDFISSHVIELMIPG 303

Query: 302 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISE 361
           EETVPMAMTLAVKFLSD PVALAQ MEEN ELKKQKD SGEEYTWTD+MSL FTQNVISE
Sbjct: 304 EETVPMAMTLAVKFLSDSPVALAQTMEENKELKKQKDSSGEEYTWTDFMSLQFTQNVISE 363

Query: 362 TLRMANIINGVWRKAQKD----------------------------------------GI 421
           TLRMANIINGVWRKAQKD                                         I
Sbjct: 364 TLRMANIINGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDEENYANPHQFNPWRWEEI 423

Query: 422 GSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRK 446
           GS +NN NFTPFGGGQRLCPGIELARLEISIFLH LVTTYKW AE+DEIIHFPTV+MRRK
Sbjct: 424 GSATNNHNFTPFGGGQRLCPGIELARLEISIFLHCLVTTYKWRAEKDEIIHFPTVKMRRK 467

BLAST of Carg16069 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.3e-153
Identity = 289/483 (59.83%), Postives = 348/483 (72.05%), Query Frame = 0

Query: 8   WVWFLSVVALACLLFKNNNNNTTNNNKIHIQNKPG-IPKGSLGWPFLGETLAFIASGYSS 67
           W+W         L  +N+     +  + + + K G IP GSLGWP +GETL FIA GYSS
Sbjct: 42  WLW---------LRLRNSKTKDGDEEEDNEEKKKGMIPNGSLGWPVIGETLNFIACGYSS 101

Query: 68  RPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITELLGS 127
           RPVTFM+KRKSLYG VFKTNI+GTPII+STD++VNKVVLQNHGNTFVPAYPKSITELLG 
Sbjct: 102 RPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKVVLQNHGNTFVPAYPKSITELLGE 161

Query: 128 FSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYVQTETKQ 187
            SIL +NG  QKR+H++IGAFL+SP LK RIT DI+ +V L L++W    LV+VQ E K+
Sbjct: 162 NSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIEASVVLTLASWAQLPLVHVQDEIKK 221

Query: 188 ITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLKIVS 247
           +TFE+LVKVLMS  PGED+ ILK EFEEFIK LICIP+K PGTRLYKSLKA+E+L+K+V 
Sbjct: 222 MTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICIPIKFPGTRLYKSLKAKERLIKMVK 281

Query: 248 KIVEERRSTAAAAAAEEKIPPRDAVDVLLQD--NDVVQGLPLDFISSHIIELMIPGEETV 307
           K+VEER+      A     P  D VDVLL+D  +   Q  P DF+S  I+E+MIPGEET+
Sbjct: 282 KVVEERQ-----VAMTTTSPANDVVDVLLRDGGDSEKQSQPSDFVSGKIVEMMIPGEETM 341

Query: 308 PMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISETLRM 367
           P AMTLAVKFLSD PVALA+++EENME+K++K + GEEY WTDYMSL FTQNVI+ETLRM
Sbjct: 342 PTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRM 401

Query: 368 ANIINGVWRKAQKDG-----------------------------------------IGSG 427
           ANIINGVWRKA KD                                           GS 
Sbjct: 402 ANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSA 461

Query: 428 SNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRKLPI 447
           +++  FTPFGGGQRLCPG+EL++LEISIFLHHLVT Y W AE DEI+ FPTV+M+R+LPI
Sbjct: 462 NSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTAEEDEIVSFPTVKMKRRLPI 510

BLAST of Carg16069 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 392.9 bits (1008), Expect = 3.5e-109
Identity = 212/464 (45.69%), Postives = 301/464 (64.87%), Query Frame = 0

Query: 23  KNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFIASGYSSRPVTFMNKRKSLYGSV 82
           K N+++ T+ +     + P  P GSLGWP +GET+ F++S YS RP +FM+KR+ +YG V
Sbjct: 36  KLNDHHVTSQS-----HGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRV 95

Query: 83  FKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITELLGSFSILQMNGNFQKRIHS 142
           FK++I GT  IVSTD++VN+ VLQ+    FVP YPK++ EL+G  SIL +NG+  +R H 
Sbjct: 96  FKSHIFGTATIVSTDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHG 155

Query: 143 IIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYVQTETKQITFEVLVKVLMSVGPG 202
           ++G+FLKSP LKA+I  D+   +   +  W+  + V +Q  +K + F+VL K L+SV  G
Sbjct: 156 LVGSFLKSPLLKAQIVRDMHKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKG 215

Query: 203 EDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLKIVSKIVEER-RSTAAAAAA 262
           EDL+ LKREFE FI  L+ +P+  PGT+L++SL+A++ ++K V +I+E + R T      
Sbjct: 216 EDLEELKREFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKT--KNKE 275

Query: 263 EEKIPPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLSDCPVA 322
           E+ +  +D VDVLL+D+   + L  + I++++I++MIPG ++VP+ +TLAVKFLSD P A
Sbjct: 276 EDDVIAKDVVDVLLKDSS--EHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAA 335

Query: 323 LAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISETLRMANIINGVWRKAQKDGIG 382
           L  + EENM+LK  K+ +GE   W DY+SL FTQ VI+ETLRM N+I GV RKA KD   
Sbjct: 336 LNLLTEENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEI 395

Query: 383 SG--------------------------------------SNNQNFTPFGGGQRLCPGIE 442
            G                                       N  +F+PFGGGQRLCPG++
Sbjct: 396 KGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLD 455

Query: 443 LARLEISIFLHHLVTTYKWGAERDEIIHFPTVRMRRKLPIAIAK 448
           LARLE S+FLHHLVT ++W AE D II+FPTV M+ KLPI I +
Sbjct: 456 LARLETSVFLHHLVTRFRWIAEEDTIINFPTVHMKNKLPIWIKR 490

BLAST of Carg16069 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 2.3e-76
Identity = 170/449 (37.86%), Postives = 251/449 (55.90%), Query Frame = 0

Query: 42  GIPKGSLGWPFLGETLAFIASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVN 101
           G+P GSLG P +GET   I +  +  P  F+++R + YGSVF T++ G P I S D + N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 102 KVVLQNHGNTFVPAYPKSITELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDI 161
           + VLQN G  F  +YP SI  LLG  S+L M G+  KR+HS+  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 162 QNTVKLRLSNWNTSKLVYVQTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALIC 221
              V+  L +W  S  V +  E K+ITFE+ VK LMS  PGE  + L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 222 IPVKLPGTRLYKSLKAREKLLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVV 281
           +P+ L  T   K+++AR K+ + ++ +V +RR      A  +K    D +  LL  +D  
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKK----DMLAALLAADD-- 269

Query: 282 QGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGE 341
            G   + I   ++ L++ G ET    MTLAVKFL++ P+ALAQ+ EE+ +++  K DS  
Sbjct: 270 -GFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-Y 329

Query: 342 EYTWTDYMSLLFTQNVISETLRMANIINGVWRKA-------------------------- 401
              W+DY S+ FTQ V++ETLR+ANII GV+R+A                          
Sbjct: 330 SLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHL 389

Query: 402 ----------------QKDGIGSGSNNQNFTPFGGGQRLCPGIELARLEISIFLHHLVTT 448
                           Q + + +G +N  FTPFGGG RLCPG ELAR+ +S+FLH LVT 
Sbjct: 390 DPNHFKDARTFNPWRWQSNSVTTGPSNV-FTPFGGGPRLCPGYELARVALSVFLHRLVTG 449

BLAST of Carg16069 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-66
Identity = 160/499 (32.06%), Postives = 252/499 (50.50%), Query Frame = 0

Query: 17  LACLLFKNNNNNTTNNNKIHIQNKPGIPKGSLGWPFLGETLAFIASGYSSRPVTFMNKRK 76
           L  +L K  N  T  N          +P G  GWPFLGET+ ++    ++    FM +  
Sbjct: 23  LFLILLKRRNRKTRFN----------LPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHV 82

Query: 77  SLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITELLGSFSILQMNGNF 136
           S YG ++++N+ G P IVS D+ +N+ +LQN G  F  +YP+SI  +LG +S+L + G+ 
Sbjct: 83  SKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDM 142

Query: 137 QKRIHSIIGAFLKSPSLKARITTDIQNTVKLRLSNWNTSKLVYVQTETKQITFEVLVKVL 196
            + + SI   FL    L+  +  D++      L +W  + +   Q E K+ TF ++ K +
Sbjct: 143 HRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHI 202

Query: 197 MSVGPG-EDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLKIVSKIVEERRST 256
           MS+ PG E+ + LK+E+  F+K ++  P+ LPGT  +K+L++R  +LK + + +EER+  
Sbjct: 203 MSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLD 262

Query: 257 AAAAAAEE---------KIPPRDAVDVLLQDNDVV------QGLPLDFISSHIIELMIPG 316
                 EE         ++   D V     D+D++        L  + I   I+ L+  G
Sbjct: 263 IKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAG 322

Query: 317 EETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGE-EYTWTDYMSLLFTQNVIS 376
            ET  +A+ LA+ FL  CP A+ ++ EE++E+ + K + GE E  W DY  + FTQ VI+
Sbjct: 323 HETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVIN 382

Query: 377 ETLRMANIINGVWRKAQKD----------------------------------------- 436
           ETLR+ N++  + RKA KD                                         
Sbjct: 383 ETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQ 442

Query: 437 ------GIGSGSNN---QNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWG-AERDE 448
                   GSGS +    N+ PFGGG RLC G ELA+LE+++F+HHLV  + W  AE D+
Sbjct: 443 QNNGASSSGSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDK 502

BLAST of Carg16069 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 3.0e-60
Identity = 135/337 (40.06%), Postives = 199/337 (59.05%), Query Frame = 0

Query: 42  GIPKGSLGWPFLGETLAFIASGYSSRPVTFMNKRKSLYGSVFKTNILGTPIIVSTDSDVN 101
           G+P GSLG P +GET   I +  +  P  F+++R + YGSVF T++ G P I S D + N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 102 KVVLQNHGNTFVPAYPKSITELLGSFSILQMNGNFQKRIHSIIGAFLKSPSLKARITTDI 161
           + VLQN G  F  +YP SI  LLG  S+L M G+  KR+HS+  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 162 QNTVKLRLSNWNTSKLVYVQTETKQITFEVLVKVLMSVGPGEDLKILKREFEEFIKALIC 221
              V+  L +W  S  V +  E K+ITFE+ VK LMS  PGE  + L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 222 IPVKLPGTRLYKSLKAREKLLKIVSKIVEERRSTAAAAAAEEKIPPRDAVDVLLQDNDVV 281
           +P+ L  T   K+++AR K+ + ++ +V +RR      A  +K    D +  LL  +D  
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKK----DMLAALLAADD-- 269

Query: 282 QGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLSDCPVALAQMMEENMELKKQKDDSGE 341
            G   + I   ++ L++ G ET    MTLAVKFL++ P+ALAQ+ EE+ +++  K DS  
Sbjct: 270 -GFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-Y 329

Query: 342 EYTWTDYMSLLFTQNVISETLRMANIINGVWRKAQKD 379
              W+DY S+ FTQ V++ETLR+ANII GV+R+A  D
Sbjct: 330 SLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTD 356